A digital microfluidic sequencing chip structure and a preparation method thereof

Abstract

The embodiment of the application provides a digital microfluidic sequencing chip structure and a preparation method thereof. The digital microfluidic sequencing chip structure comprises a first substrate and a second substrate arranged oppositely, the first substrate and the second substrate enclose a containing cavity, the containing cavity comprises an outlet flow channel layer close to the first substrate and a liquid inlet flow channel layer close to the second substrate, and a sequencing structure layer for performing single-cell sequencing is located between the liquid inlet flow channel layer and the outlet flow channel layer. The sequencing structure layer is provided with one or more than one channel communicating with the liquid inlet flow channel layer and the outlet flow channel layer, one or more than one capture element is arranged in each channel, the capture element is used for capturing DNA or RNA of a single cell, and the inner wall of the channel is provided with a library construction reagent layer. The library construction reagent layer is used for performing targeted library construction on the DNA or the RNA, the automation capability of single-cell sample processing is effectively improved, the operation efficiency of sample processing is improved, and an effective tool is provided for sample processing of molecular diagnosis.

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