Preparation method of electrospun core-shell nanofiber membrane for early diagnosis marker detection of prostate cancer

Graphene oxide composite nanofiber membranes were prepared by electrospinning and electric field induction, and combined with a targeted chain release strategy, which solved the sensitivity and efficiency problems of existing electrochemical biosensors in the detection of prostate cancer biomarkers, and achieved rapid and accurate PSA detection, which is suitable for early diagnosis and clinical applications.

CN117705907BActive Publication Date: 2025-10-17NANJING TECH UNIV
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Patent Information

Application Number
CN202311696916.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-12-12
Publication Date
2025-10-17
Estimated Expiration
2043-12-12

AI Technical Summary

Technical Problem

Existing electrochemical biosensors suffer from low sensitivity, high cost, cumbersome operation, and long detection time in the detection of prostate cancer biomarkers. Furthermore, the conductivity and catalytic activity of nanofiber membrane materials prepared by electrospinning technology are not ideal, which affects the detection efficiency.

Method used

Graphene oxide composite nanofiber membranes were prepared using electrospinning and electric field induction. Carboxylated electrospun core-shell nanofiber membranes were prepared by thermal reduction and plasma bombardment. Combined with a targeted chain release strategy, a high-performance aptamer biosensor was designed.

Benefits of technology

It achieves rapid and selective identification of the prostate cancer marker PSA, with a detection limit as low as 0.3 pg/mL, a wide linear range, and can complete the detection within 4 minutes. It has good accuracy and stability and is suitable for the detection of complex serum samples.

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Abstract

The application belongs to the technical field of clinical diagnosis and relates to a preparation method of an electrospun core-shell nanofiber membrane for rapid early diagnosis of prostate cancer. The preparation process comprises preparation of an electrospun core-shell nanofiber membrane, preparation of a carboxylated electrospun core-shell nanofiber membrane and preparation of a PSA aptamer biosensor based on PAN@RGO-COOH. The detection limit of the constructed electrochemical PSA aptamer sensor is 0.3 pg / mL, the linear range is 1 pg / mL-295 ng / mL, and the sensor has good repeatability, stability and anti-interference property. The aptamer sensor shows great potential in early warning of prostate cancer and clinical screening of cancer biomarkers, and can be expanded into an accurate analysis platform for various target analytes.
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