快速鉴定番茄“冬韵”杂交种子纯度的分子标记及方法
By employing KASP technology and high-throughput DNA extraction methods, and utilizing polymorphic primers between parents, the purity of tomato hybrid seeds was identified. This solved the problems of time-consuming and complex operations in traditional methods, and enabled rapid and accurate seed purity detection.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHANDONG ACADEMY OF AGRICULTURAL SCIENCES
- Filing Date
- 2024-02-04
- Publication Date
- 2026-07-17
AI Technical Summary
In existing technologies, methods for identifying the purity of tomato hybrid seeds are time-consuming, susceptible to environmental influences, and complex to operate. Traditional DNA extraction has a long cycle and uses toxic reagents, leading to delayed seed sales and inaccurate identification.
A molecular marker method based on KASP technology was used, with PCR amplification performed using inter-parent polymorphic primers F1 and F2 and universal primer R. Combined with a high-throughput DNA extraction method, DNA was extracted at the cotyledon stage of tomato, and the purity of seeds was rapidly and efficiently identified by fluorescence detection.
It shortens the testing cycle, improves identification efficiency and accuracy, simplifies operation, reduces costs, is suitable for large-scale testing, and enables rapid and accurate identification of seed purity.
Smart Images
Figure CN117737294B_ABST