A method and apparatus for alkaline sterilization of fermentation sample broth

Abstract

This invention provides a method and apparatus for alkaline sterilization of fermentation sample liquid, applied to sterilization equipment. The sterilization equipment includes: a sterilization tank, a pH measuring device, a stirring device, and an alkaline input device. The stirring device and the pH measuring device are assembled in the sterilization tank. The method includes: controlling the alkaline input device to input alkaline solution into the sterilization tank in batches; controlling the stirring device to stir the liquid in the sterilization tank and measuring the pH of the liquid in the sterilization tank using the pH measuring device; when the pH measured by the pH measuring device exceeds a first preset threshold for a third preset time, controlling the alkaline input device to stop inputting alkaline solution, controlling the stirring device to stop stirring, and discharging the liquid from the sterilization tank.

Description

Technical Field

[0001] This invention relates to the field of sterilization, and more particularly to an alkaline sterilization method and apparatus for fermentation sample broth. Background Technology

[0002] Microbial liquid fermentation is a complex process involving microorganisms. Biochemical indicators, including the concentration of glucose (the fermentation feedstock) and the concentrations of various fermentation products and intermediates, are crucial parameters in the fermentation process. In large-scale industrial fermentation production, pilot-scale, and laboratory production, regular sampling is necessary after fermentation begins to monitor microbial growth, i.e., cell concentration (OD value), the consumption of various components in the culture medium, especially carbon and nitrogen sources, and the formation of metabolites during growth. Understanding these parameters allows for the assessment of various biochemical indicators during the culture process, thereby determining microbial growth, contamination status, and the need for feed replenishment. Sampling is a critical step in the fermentation process. To avoid adverse environmental impacts from microorganisms, live discharge from the fermentation workshop is strictly prohibited; the sampled liquid must be sterilized before discharge.

[0003] In related technologies, the process is generally carried out by moist heat sterilization. This method involves setting up a large sampling sterilization tank, inputting the sampling liquid into the sterilization tank, and introducing saturated steam. After reaching a certain temperature, the timer starts, and after a certain period of time, the sampling liquid in the sterilization tank is considered to have been sterilized and can be discharged.

[0004] However, this method requires operator intervention during sterilization. During steam sterilization, the operator must constantly monitor the temperature changes of the sterilization tank to prevent overheating and overpressure caused by leaving the steam valve open for extended periods, which could damage the sterilization equipment. Therefore, this method is relatively dangerous, carrying risks such as overheating and overpressure due to delayed operation during heating, equipment damage, and burns. Furthermore, to reduce heat loss and improve steam utilization, the sterilization tank needs to be insulated, which increases the equipment's investment cost. Summary of the Invention

[0005] In view of this, the present invention provides a method and apparatus for alkaline sterilization of fermentation sample liquid to overcome the deficiencies in related technologies.

[0006] Specifically, the present invention is achieved through the following technical solution:

[0007] According to a first aspect of the present invention, an alkaline sterilization method for fermentation sample broth is provided, applied to a sterilization device, the sterilization device comprising: a sterilization tank, a pH measuring device, a stirring device, and an alkaline input device, wherein the stirring device and the pH measuring device are assembled in the sterilization tank; the method comprises:

[0008] In response to the sampling liquid in the sterilization tank reaching a preset level, the alkaline solution input device is controlled to input alkaline solution into the sterilization tank in batches; wherein, the duration of each alkaline solution input by the alkaline solution input device is a first preset duration;

[0009] The stirring device is controlled to stir the liquid in the sterilization tank, and the pH of the liquid in the sterilization tank is measured by the pH measuring device; wherein, during the process of the alkali input device inputting alkali in batches, the condition for inputting alkali again is that the pH measured by the pH measuring device has not changed within a second preset time period;

[0010] If the pH value measured by the pH measuring device exceeds the first preset threshold for a period of time that reaches the third preset time, the alkaline solution input device is controlled to stop inputting alkaline solution, the stirring device is controlled to stop stirring, and the liquid in the sterilization tank is discharged.

[0011] According to a second aspect of the present invention, a sterilization apparatus is provided, the sterilization apparatus comprising:

[0012] Sterilization tank, used to hold the sampling liquid to be sterilized and the alkaline solution used for sterilization;

[0013] An alkali input device, connected to the sterilization tank, is used to input alkali solution into the sterilization tank in batches in response to the sampling liquid in the sterilization tank reaching a preset liquid level; when the duration for which the pH measured by the pH measuring device exceeds a first preset threshold reaches a third preset duration, the alkali input device stops inputting alkali solution; wherein, the duration for each alkali input by the alkali input device is the first preset duration;

[0014] A pH measuring device is installed in the sterilization tank to measure the pH of the liquid in the sterilization tank; wherein, during the process of batch input of alkali solution by the alkali solution input device, the condition for inputting alkali solution again is that the pH measured by the pH measuring device has not changed within a second preset time period;

[0015] A stirring device, assembled in the sterilization tank, is used to stir the liquid in the sterilization tank; when the pH value measured by the pH measuring device exceeds the first preset threshold for a period of time that reaches the third preset time, the stirring device stops stirring.

[0016] According to a third aspect of the present invention, an alkaline sterilization device for fermentation sample broth is provided, applied to sterilization equipment, the sterilization equipment comprising: a sterilization tank, a pH measuring device, a stirring device, and an alkaline input device, wherein the stirring device and the pH measuring device are assembled in the sterilization tank; the device comprises:

[0017] First control unit: In response to the sampling liquid in the sterilization tank reaching a preset liquid level, controls the alkali input device to input alkali solution into the sterilization tank in batches; wherein, the duration of each alkali input by the alkali input device is a first preset duration;

[0018] Second control unit: controls the stirring device to stir the liquid in the sterilization tank, and measures the pH of the liquid in the sterilization tank through the pH measuring device; wherein, during the process of the alkali input device inputting alkali in batches, the condition for inputting alkali again is that the pH measured by the pH measuring device has not changed within a second preset time period;

[0019] Drainage Unit: When the pH value measured by the pH measuring device exceeds the first preset threshold for a period of time exceeding the first preset threshold for a third preset period of time, the unit controls the alkali input device to stop inputting alkali, controls the stirring device to stop stirring, and drains the liquid from the sterilization tank.

[0020] According to a fourth aspect of the present invention, an electronic device is provided, comprising:

[0021] processor;

[0022] Memory used to store processor-executable instructions;

[0023] The processor implements the method as described in any one of the first aspects by executing the executable instructions.

[0024] According to a fifth aspect of the present invention, a computer-readable storage medium is provided having a computer program stored thereon, which, when executed by a processor, implements the steps of any of the methods described in the first aspect.

[0025] The technical solutions provided by the embodiments of the present invention may include the following beneficial effects:

[0026] In embodiments of the present invention, on the one hand, alkaline sterilization is less risky and less costly than steam sterilization, and the sterilization of the fermentation sample broth is completed without operator intervention throughout the process. On the other hand, during the alkaline sterilization process, the sample broth is stirred by a stirring device, ensuring complete inactivation and avoiding incomplete sterilization due to uneven mixing of the sample broth and alkaline solution. Furthermore, the alkaline solution is not added continuously to the sterilization equipment but in batches, and the pH of the sample broth is continuously measured by a pH measuring device. This not only allows for better control of the pH of the sample broth but also saves on the amount of alkaline solution input, thereby reducing costs. Attached Figure Description

[0027] To more clearly illustrate the technical solutions of the present invention, the accompanying drawings used in the description of the embodiments or prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention, and those skilled in the art can obtain other drawings based on these drawings without any creative effort.

[0028] Figure 1 This is a flowchart illustrating an alkaline sterilization method for fermentation sample liquid, as shown in an embodiment of the present invention.

[0029] Figure 2 This is a schematic diagram of a sterilization device shown in an embodiment of the present invention;

[0030] Figure 3 This is a schematic structural diagram of an electronic device shown in an embodiment of the present invention;

[0031] Figure 4 This is a block diagram of an alkaline sterilization device for fermentation sample liquid, as shown in an embodiment of the present invention. Detailed Implementation

[0032] Exemplary embodiments will now be described in detail, examples of which are illustrated in the accompanying drawings. When the following description relates to the drawings, unless otherwise indicated, the same numbers in different drawings denote the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present invention. Rather, they are merely examples of apparatuses and methods consistent with some aspects of the present invention.

[0033] The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular forms “a,” “the,” and “the” used in this invention and the appended claims are also intended to include the plural forms unless the context clearly indicates otherwise. It should also be understood that the term “and / or” as used herein refers to and includes any or all possible combinations of one or more of the associated listed items.

[0034] It should be understood that although the terms first, second, third, etc., may be used in this invention to describe various information, this information should not be limited to these terms. These terms are only used to distinguish information of the same type from one another. For example, first information may also be referred to as second information without departing from the scope of this invention, and similarly, second information may also be referred to as first information. Depending on the context, the word "if" as used herein may be interpreted as "when," "when," or "in response to a determination."

[0035] The following is a detailed description of an embodiment of the alkaline sterilization method for fermentation sample liquid according to the present invention, with reference to the accompanying drawings.

[0036] Microbial liquid fermentation is a complex process involving microorganisms. Biochemical indicators, including the concentration of glucose (the fermentation feedstock) and the concentrations of various fermentation products and intermediates, are crucial parameters in the fermentation process. In large-scale industrial fermentation production, pilot-scale, and laboratory production, regular sampling is necessary after fermentation begins to monitor microbial growth, i.e., cell concentration (OD value), the consumption of various components in the culture medium, especially carbon and nitrogen sources, and the formation of metabolites during growth. Understanding these parameters allows for the assessment of various biochemical indicators during the culture process, thereby determining microbial growth, contamination status, and the need for feed replenishment. Sampling is a critical step in the fermentation process. To avoid adverse environmental impacts from microorganisms, live discharge from the fermentation workshop is strictly prohibited; the sampled liquid must be sterilized before discharge.

[0037] In related technologies, the process is generally carried out by moist heat sterilization. This method involves setting up a large sampling sterilization tank, inputting the sampling liquid into the sterilization tank, and introducing saturated steam. After reaching a certain temperature, the timer starts, and after a certain period of time, the sampling liquid in the sterilization tank is considered to have been sterilized and can be discharged.

[0038] However, this method requires operator intervention during sterilization. During steam sterilization, the operator must constantly monitor the temperature changes of the sterilization tank to prevent overheating and overpressure caused by leaving the steam valve open for extended periods, which could damage the sterilization equipment. Therefore, this method is relatively dangerous, carrying risks such as overheating and overpressure due to delayed operation during heating, equipment damage, and burns. Furthermore, to reduce heat loss and improve steam utilization, the sterilization tank needs to be insulated, which increases the equipment's investment cost.

[0039] To address the shortcomings in related technologies, this specification proposes an alkaline sterilization method for fermentation sample broth.

[0040] Figure 1 This is a flowchart illustrating an exemplary embodiment of the present invention for an alkaline sterilization method of a fermentation sample. The method is applied to a sterilization device, which includes: a sterilization tank, a pH measuring device, a stirring device, and an alkaline input device. The stirring device and the pH measuring device are assembled in the sterilization tank. Specifically, the method may include the following steps:

[0041] Step 102: In response to the sampling liquid in the sterilization tank reaching a preset liquid level, the alkaline solution input device is controlled to input alkaline solution into the sterilization tank in batches; wherein, the duration of each alkaline solution input by the alkaline solution input device is a first preset duration.

[0042] Specifically, this method can be applied to the control module of sterilization equipment. This control module can be a central control chip installed on the sterilization equipment. This central control chip can be connected to various devices included in the sterilization equipment to receive signals from each device and send control signals to each device to control different devices to perform corresponding operations. For example, the control chip can send a start signal to the stirring device to start stirring the liquid in the sterilization tank; or, the control chip can receive signals from a pH measuring device and determine the pH of the liquid in the sterilization tank based on the received signals.

[0043] Sterilization equipment can be used to sterilize fermentation sample broth. For example... Figure 2 As shown, the sterilization equipment may include: a sterilization tank 201, a pH measuring device 202, a stirring device 203, and an alkali input device 204. The sterilization tank 201 serves as a container and can be used as the sterilization site for the sample solution. The sterilization tank 201 is connected to the alkali input device 204, allowing the sterilization equipment to input alkali solution into the sterilization tank 201 via the alkali input device 204, mixing the alkali solution with the sample solution in the sterilization tank 201 to achieve sterilization. The alkali input device 204 does not continuously input alkali solution into the sterilization tank 201, but rather inputs it in batches, with each input lasting for a first preset duration. This first preset duration can be set in advance by the operator of the sterilization equipment, for example, it can be set to 2 minutes.

[0044] The pH measuring device 202 can be installed inside the sterilization tank 201 to measure the pH of the liquid in the sterilization tank 201. This pH measuring device 202 can be of various types, and this manual does not limit its application.

[0045] The stirring device 203 can be mounted on the sterilization tank 201. The rotor 2031 can be mounted inside the sterilization tank 201 for stirring the liquid contained within it. Other parts of the stirring device 203 can be mounted outside the sterilization tank 201. These other parts may include a signal transceiver module for receiving and sending signals to the control chip; they may also include a power module for providing power to the signal transceiver module. The specific structure of the stirring device 203 is related to its drive mechanism. In one case, the stirring device 203 is a stirring motor driven by electricity, in which case the power module can also provide power to the rotor. In another case, the stirring device 203 is a magnetic stirrer driven by magnetism. Its basic principle is based on the repulsion and attraction of like poles in a magnetic field, using the magnetic field to drive a magnetic rotor placed in the container to rotate in a circular motion, thereby achieving the purpose of stirring the liquid. Compared to electric mixers, magnetic stirrers take up less space, are cheaper, are more suitable for mixing small containers, and are simple to operate, install, and maintain.

[0046] Step 104: Control the stirring device to stir the liquid in the sterilization tank, and measure the pH of the liquid in the sterilization tank through the pH measuring device; wherein, during the process of the alkali input device inputting alkali in batches, the condition for inputting alkali again is that the pH measured by the pH measuring device has not changed within a second preset time period.

[0047] During the process of inputting alkali solution into the sterilization tank 201 via the alkali solution input device 204, the input alkali solution cannot be instantly and uniformly mixed with the sampling solution. Only with continuous stirring by the stirring device 203 can the alkali solution gradually mix with the sampling solution. Therefore, the pH measured by the pH measuring device 202 is not constant, but gradually stabilizes as the alkali solution mixes with the sampling solution. The operator of the sterilization equipment can preset a second time interval, for example, 10 seconds. After the pH measured by the pH measuring device 202 remains constant for 10 seconds, the alkali solution input device 204 will continue to input alkali solution. Specifically, the pH measuring device 202 may include a timing device to time the duration of pH measurement; or, the pH measuring device 202 may send a signal containing pH information to the control chip at regular intervals, where this interval is less than the second preset time interval. The control chip can calculate the duration of pH measurement based on the time interval between the signals sent by the pH measuring device 202.

[0048] Step 106: If the pH value measured by the pH measuring device exceeds the first preset threshold for a period of time that reaches the third preset time, control the alkali input device to stop inputting alkali, control the stirring device to stop stirring, and discharge the liquid in the sterilization tank.

[0049] The first preset threshold can be preset by the operator of the sterilization equipment, for example, it can be set to pH=11. When the pH of the sample solution exceeds the first preset threshold for a period of time that reaches the third preset time (which can be set to 30 minutes), it indicates that the bacteria in the sample solution have been in a strongly alkaline environment for the third preset time, and this manual considers the sample solution to have been sterilized at this point.

[0050] In this embodiment, on the one hand, alkaline sterilization is less risky and less expensive than steam sterilization, and the sterilization of the fermentation sample broth is completed without operator intervention throughout the process. On the other hand, during the alkaline sterilization process, a stirring device is used to agitate the sample broth, ensuring complete inactivation and avoiding incomplete sterilization due to uneven mixing of the sample broth and alkaline solution. Furthermore, the alkaline solution is added to the sterilization equipment in batches, not continuously, and the pH of the sample broth is continuously measured using a pH measuring device. This not only allows for better control of the sample broth's pH but also reduces the amount of alkaline solution required, thereby lowering costs.

[0051] In one embodiment, the sterilization equipment further includes: a liquid level measuring device and a sampling liquid input device; the method further includes: inputting sampling liquid into the sterilization tank through the sampling liquid input device; measuring the liquid level of the sampling liquid input into the sterilization tank through the liquid level measuring device, and controlling the sampling liquid input device to stop inputting sampling liquid when the measured liquid level of the sampling liquid reaches the preset liquid level.

[0052] like Figure 2 As shown, the sterilization equipment also includes a liquid level measuring device 205 and a sampling liquid input device 206. The liquid level measuring device 205 measures the liquid level in the sterilization tank 202. When sampling liquid is input into the sampling liquid input device 206, the liquid level measuring device 205 continuously measures the liquid level. When the liquid level reaches a preset level (e.g., three-quarters of the sterilization tank), the liquid level measuring device 205 can send a signal to the control chip. At this time, the control chip can send a stop-infusion signal to the sampling liquid input device 206 to control the sampling liquid input device 206 to stop inputting sampling liquid. When alkali solution is input into the alkali solution input device 204, the liquid level measuring device 205 can also continue to measure the liquid level. When the liquid level of the mixture of alkali solution and sampling liquid reaches a certain level (e.g., 95% of the sterilization tank), the liquid level measuring device 205 can send a signal to the control chip. At this point, regardless of whether the sampling solution has been sterilized, the control chip needs to send a stop infusion signal to the alkali input device 204 to control the alkali input device 204 to stop inputting alkali, thereby avoiding liquid overflow.

[0053] The liquid level measuring device 205 can be a remote liquid level gauge (such as the BL-FO type remote liquid level gauge). The remote liquid level gauge is a device specifically designed for liquid level measurement, which is developed and manufactured by ingeniously combining the characteristics of mechanical transmission with the principles of magnetic field and Archimedes (the law of buoyancy).

[0054] In this embodiment, by installing a liquid level measuring device in the sterilization equipment, the liquid level measuring device can measure the liquid level in the sterilization tank, thereby reducing the risk of tank explosion due to liquid overflow.

[0055] Furthermore, the sampling liquid input device includes a flow control switch and a flow measurement device; the method further includes: measuring the flow rate of the sampling liquid input into the sampling liquid input device using the flow measurement device; if the measured flow rate exceeds a first preset flow rate value, controlling the flow control switch to reduce the flow rate of the sampling liquid input into the sampling liquid input device; if the measured flow rate is less than a second preset flow rate value, controlling the flow control switch to increase the flow rate of the sampling liquid input into the sampling liquid input device.

[0056] Both the first preset flow rate value and the second preset flow rate value can be set in advance by the operator of the sterilization equipment, and the first preset flow rate value is greater than or equal to the second preset flow rate value.

[0057] like Figure 2 As shown, the sampling liquid input device 206 includes a flow control switch 2061 and a flow measurement device 2062. The flow measurement device 2062 can measure the flow rate of the input sampling liquid and send a corresponding signal to the control chip according to the measured flow rate, so that the control chip controls the flow control switch 2061 to adjust the flow rate of the input sampling liquid according to the received signal.

[0058] Specifically, both the first and second preset flow rates can be 1 L / min. The flow measurement device 2062 can be a magnetic induction device installed in the sampling liquid input pipeline. This magnetic induction device includes a magnetic core, a magnetic source, and a signal transceiver. If the sampling liquid flow rate is greater than 1 L / min, the magnetic core can be displaced under the action of the liquid flow rate, which drives the magnetic source to generate a magnetic control effect, causing the signal transceiver to output a "1" switch signal, which is sent to the control chip. If the sampling liquid flow rate is less than 1 L / min, the magnetic core drives the magnetic source back to its original position under the push of the reset spring, causing the signal transceiver to output a "0" switch signal. When the control chip receives a "1" switch signal, it controls the flow control switch 2061 to decrease the flow rate; when it receives a "0" switch signal, it controls the flow control switch 2061 to increase the flow rate.

[0059] In this embodiment, by setting a flow measurement device and a flow control switch in the sampling liquid input device, the sterilization equipment can measure the flow rate of the input sampling liquid and control the flow rate, thereby avoiding the situation where the liquid level control is not timely due to the excessive flow rate of the input sampling liquid and exceeds the preset liquid level, or avoiding the situation where the sterilization efficiency is too low due to the insufficient flow rate of the input sampling liquid.

[0060] Of course, the alkali input device 204 may also include a flow control switch 2041 to control the flow rate of the alkali input, which will not be elaborated here.

[0061] In one embodiment, the method further includes: when the pH of the liquid reaches a second preset threshold, modifying the duration of each input of alkaline solution by the alkaline solution input device to a fourth preset duration; wherein the first preset threshold is greater than the second preset threshold, and the first preset duration is greater than the fourth preset duration.

[0062] With the first preset duration being 2 minutes, the fourth preset duration can be 30 seconds. Reducing the alkali input time means slowing down the alkali input rate. The second preset threshold can be set close to the first preset threshold; for example, when the first preset threshold is pH=11, the second preset threshold can be set to pH=10. When the pH is close to the first preset threshold, the control chip can slow down the alkali input rate. This helps the sterilization equipment to accurately control the pH of the liquid in the sterilization tank and reduces alkali waste, thereby lowering costs.

[0063] In one embodiment, the sterilization equipment further includes a feeding pump, which is connected to the drain port of the sterilization tank via a drain pipe, and the drain pipe is equipped with a pneumatic switch ball valve; the step of discharging the liquid in the sterilization tank includes: opening the pneumatic switch ball valve and starting the feeding pump so that the feeding pump draws out the liquid in the sterilization tank.

[0064] like Figure 2 As shown, the sterilization equipment also includes a feed pump 207, which is connected to the drain port of the sterilization tank 201 via a drain pipe. A pneumatic ball valve 208 is installed on the drain pipe. After opening the pneumatic ball valve 208, the drain pipe can drain normally, and the feed pump 207 can draw liquid from the sterilization tank 201 from the drain pipe, thereby achieving the discharge of the sterilized sample liquid.

[0065] At this time, the liquid level measuring device 205 can measure the liquid level in the sterilization tank 201. If the liquid level drops to 0, it indicates that the liquid has been completely discharged. The sampling liquid input device 206 can then be controlled to input the sampling liquid to continue processing the next batch of sampling liquid.

[0066] This manual also proposes a sterilization device, such as... Figure 2 As shown, the sterilization equipment includes:

[0067] Sterilization tank 201 is used to hold the sampling liquid to be sterilized and the alkaline solution used for sterilization;

[0068] Alkali input device 204, connected to the sterilization tank 201, is used to input alkali solution into the sterilization tank 201 in batches in response to the sampling liquid in the sterilization tank 201 reaching a preset liquid level; when the pH measured by the pH measuring device 202 exceeds the first preset threshold for a period of time that reaches a third preset time, the alkali input device 204 stops inputting alkali solution; wherein, the duration of each alkali input by the alkali input device 204 is the first preset time.

[0069] A pH measuring device 202 is installed in the sterilization tank 201 and is used to measure the pH of the liquid in the sterilization tank 201; wherein, during the process of batch input of alkali solution by the alkali solution input device 204, the condition for inputting alkali solution again is that the pH measured by the pH measuring device 202 has not changed within a second preset time period.

[0070] A stirring device 203 is installed in the sterilization tank 201 and is used to stir the liquid in the sterilization tank 201; when the pH value measured by the pH measuring device 202 exceeds the first preset threshold for a period of time that reaches the third preset time, the stirring device 203 stops stirring.

[0071] The sterilization equipment also includes a tail gas exhaust port 209 and a manhole 210 located on the top of the sterilization tank 201. During the mixing of the alkali solution and the sampling solution, unpleasant or harmful gases may be generated; these gases can be extracted from the tail gas exhaust port 209 for unified treatment. The manhole 210 is opened for maintenance personnel to inspect the equipment inside the sterilization tank 201.

[0072] In this embodiment, on the one hand, alkaline sterilization is less risky and less expensive than steam sterilization, and the sterilization of the fermentation sample broth is completed without operator intervention throughout the process. On the other hand, during the alkaline sterilization process, a stirring device is used to agitate the sample broth, ensuring complete inactivation and avoiding incomplete sterilization due to uneven mixing of the sample broth and alkaline solution. Furthermore, the alkaline solution is added to the sterilization equipment in batches, not continuously, and the pH of the sample broth is continuously measured using a pH measuring device. This not only allows for better control of the sample broth's pH but also reduces the amount of alkaline solution required, thereby lowering costs.

[0073] Corresponding to the embodiments of the foregoing methods, the present invention also provides embodiments of electronic devices and apparatuses.

[0074] Figure 3 This is a schematic structural diagram of an electronic device according to an embodiment of the present invention. Please refer to it. Figure 3 At the hardware level, the device includes a processor 301, a network interface 302, memory 303, non-volatile memory 304, and an internal bus 305, and may also include other hardware required for business operations. One or more embodiments of the present invention can be implemented in software, for example, the processor 301 reads the corresponding computer program from the non-volatile memory 304 into memory 303 and then runs it. Of course, in addition to software implementation, one or more embodiments of the present invention do not exclude other implementation methods, such as logic devices or a combination of hardware and software, etc. That is to say, the execution subject of the following processing flow is not limited to each logic unit, but can also be hardware or logic devices.

[0075] Figure 4 This invention provides a block diagram of an alkaline sterilization device for fermentation sample broth. Please refer to the following: Figure 4 This device can be applied to, for example Figure 4 The device shown is used in sterilization equipment, which includes: a sterilization tank, a pH measuring device, a stirring device, and an alkali input device. The stirring device and the pH measuring device are assembled in the sterilization tank to achieve the technical solution described in this invention. The device includes:

[0076] The first control unit 402 is configured to control the alkali input device to input alkali solution into the sterilization tank in batches in response to the sampling liquid in the sterilization tank reaching a preset liquid level; wherein the duration of each alkali input by the alkali input device is a first preset duration.

[0077] The second control unit 404 is used to control the stirring device to stir the liquid in the sterilization tank, and to measure the pH of the liquid in the sterilization tank by the pH measuring device; wherein, during the process of the alkali input device inputting alkali in batches, the condition for inputting alkali again is that the pH measured by the pH measuring device has not changed within a second preset time period.

[0078] The draining unit 406 is used to control the alkalinity input device to stop inputting alkalinity, control the stirring device to stop stirring, and drain the liquid in the sterilization tank when the duration for which the pH measured by the pH measuring device exceeds the first preset threshold reaches the third preset duration.

[0079] Optionally, the sterilization equipment further includes: a liquid level measuring device and a sampling liquid input device; the method further includes:

[0080] Input unit 408 is used to input sampling liquid into the sterilization tank through the sampling liquid input device;

[0081] The first measuring unit 410 is used to measure the liquid level of the sampling liquid input into the sterilization tank through the liquid level measuring device, and to control the sampling liquid input device to stop inputting the sampling liquid when the liquid level of the sampling liquid reaches the preset liquid level.

[0082] Optionally, the sampling liquid input device includes a flow control switch and a flow measurement device; the method further includes:

[0083] The second measuring unit 412 is used to measure the flow rate of the sample liquid input into the sample liquid input device through the flow measuring device;

[0084] The third control unit 414 is used to control the flow control switch to reduce the flow rate of the sampling liquid input device when the measured flow rate exceeds the first preset flow rate value.

[0085] The fourth control unit 416 is used to control the flow control switch to increase the flow rate of the sampling liquid input device when the measured flow rate is less than the second preset flow rate value.

[0086] Optionally, the method further includes:

[0087] Modification unit 418 is used to modify the duration of each input of alkaline solution by the alkaline solution input device to a fourth preset duration when the pH of the liquid reaches a second preset threshold; wherein the first preset threshold is greater than the second preset threshold, and the first preset duration is greater than the fourth preset duration.

[0088] Optionally, the sterilization equipment further includes a feeding pump, which is connected to the drain port of the sterilization tank via a drain pipe, and the drain pipe is equipped with a pneumatic ball valve; the drain unit 406 is specifically used for:

[0089] Open the pneumatic ball valve and start the feeding pump to draw liquid out of the sterilization tank.

[0090] Optionally, the stirring device is a magnetic stirrer.

[0091] While this invention contains numerous specific details, these should not be construed as limiting the scope of any invention or the scope of the claims, but rather are primarily intended to describe features of specific embodiments of particular inventions. Certain features described in the multiple embodiments of this invention may also be implemented in combination in a single embodiment. On the other hand, various features described in a single embodiment may also be implemented separately in multiple embodiments or in any suitable sub-combination. Furthermore, while features may function in certain combinations as described above and even initially claimed in this way, one or more features from a claimed combination may be removed from that combination in some cases, and a claimed combination may refer to a sub-combination or a variation of a sub-combination.

[0092] Similarly, although the operations are depicted in a specific order in the accompanying drawings, this should not be construed as requiring these operations to be performed in the specific order shown or sequentially, or requiring all illustrated operations to be performed to achieve the desired result. In some cases, multitasking and parallel processing may be advantageous. Furthermore, the separation of various system modules and components in the above embodiments should not be construed as requiring such separation in all embodiments, and it should be understood that the described program components and systems can generally be integrated together in a single software product or packaged into multiple software products.

[0093] Thus, specific embodiments of the subject matter have been described. Other embodiments are within the scope of the appended claims. In some cases, the actions recited in the claims may be performed in a different order and still achieve the desired result. Furthermore, the processes depicted in the drawings are not necessarily shown in a specific order or sequence to achieve the desired result. In some implementations, multitasking and parallel processing may be advantageous.

[0094] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for alkaline sterilization of fermentation sample broth, characterized in that, The method is applied to sterilization equipment, which includes: a sterilization tank, a pH measuring device, a stirring device, and an alkali input device, wherein the stirring device and the pH measuring device are assembled in the sterilization tank; the method includes: In response to the sampling liquid in the sterilization tank reaching a preset level, the alkaline solution input device is controlled to input alkaline solution into the sterilization tank in batches; wherein, the duration of each alkaline solution input by the alkaline solution input device is a first preset duration; The stirring device is controlled to stir the liquid in the sterilization tank, and the pH of the liquid in the sterilization tank is measured by the pH measuring device; wherein, during the process of the alkali input device inputting alkali in batches, the condition for inputting alkali again is that the pH measured by the pH measuring device has not changed within a second preset time period; If the pH value measured by the pH measuring device exceeds the first preset threshold for a period of time that reaches the third preset time, the alkaline solution input device is controlled to stop inputting alkaline solution, the stirring device is controlled to stop stirring, and the liquid in the sterilization tank is discharged.

2. The method according to claim 1, characterized in that, The sterilization equipment further includes: a liquid level measuring device and a sampling liquid input device; the method further includes: The sampling solution is input into the sterilization tank through the sampling solution input device; The liquid level measurement device measures the liquid level of the sampled liquid input into the sterilization tank, and when the liquid level of the sampled liquid reaches the preset liquid level, the sampling liquid input device is controlled to stop inputting the sampled liquid.

3. The method according to claim 2, characterized in that, The sampling liquid input device includes a flow control switch and a flow measurement device; the method further includes: The flow rate of the sample liquid input into the sample liquid input device is measured by the flow measurement device; If the measured flow rate exceeds the first preset flow rate value, the flow control switch is controlled to reduce the flow rate of the sampling liquid input device. If the measured flow rate is less than the second preset flow rate value, the flow control switch is controlled to increase the flow rate of the sampling liquid input device.

4. The method according to claim 1, characterized in that, The method further includes: When the pH of the liquid reaches a second preset threshold, the duration of each input of alkaline solution by the alkaline solution input device is modified to a fourth preset duration; wherein, the first preset threshold is greater than the second preset threshold, and the first preset duration is greater than the fourth preset duration.

5. The method according to claim 1, characterized in that, The sterilization equipment further includes a feeding pump, which is connected to the drain port of the sterilization tank via a drain pipe, and the drain pipe is equipped with a pneumatic ball valve; the step of discharging the liquid from the sterilization tank includes: Open the pneumatic ball valve and start the feeding pump to draw liquid out of the sterilization tank.

6. The method according to claim 1, characterized in that, The stirring device is a magnetic stirrer.

7. A sterilization device, characterized in that, The sterilization equipment includes: Sterilization tank, used to hold the sampling liquid to be sterilized and the alkaline solution used for sterilization; An alkali input device, connected to the sterilization tank, is used to input alkali solution into the sterilization tank in batches in response to the sampling liquid in the sterilization tank reaching a preset liquid level; when the pH value measured by the pH measuring device exceeds a first preset threshold for a period of time that reaches a third preset time, the alkali input device stops inputting alkali solution; wherein, the duration of each alkali input by the alkali input device is the first preset time. A pH measuring device is installed in the sterilization tank to measure the pH of the liquid in the sterilization tank; wherein, during the process of batch input of alkali solution by the alkali solution input device, the condition for inputting alkali solution again is that the pH measured by the pH measuring device has not changed within a second preset time period; A stirring device, assembled in the sterilization tank, is used to stir the liquid in the sterilization tank; when the pH value measured by the pH measuring device exceeds the first preset threshold for a period of time that reaches the third preset time, the stirring device stops stirring.

8. An alkaline sterilization device for fermentation sample broth, characterized in that, This device is used in sterilization equipment, which includes: a sterilization tank, a pH measuring device, a stirring device, and an alkali input device, wherein the stirring device and the pH measuring device are assembled in the sterilization tank; the device includes: First control unit: In response to the sampling liquid in the sterilization tank reaching a preset liquid level, controls the alkali input device to input alkali solution into the sterilization tank in batches; wherein, the duration of each alkali input by the alkali input device is a first preset duration; Second control unit: controls the stirring device to stir the liquid in the sterilization tank, and measures the pH of the liquid in the sterilization tank through the pH measuring device; wherein, during the process of the alkali input device inputting alkali in batches, the condition for inputting alkali again is that the pH measured by the pH measuring device has not changed within a second preset time period; Drainage unit: When the pH value measured by the pH measuring device exceeds the first preset threshold for a period of time that reaches the third preset time, the unit controls the alkali input device to stop inputting alkali, controls the stirring device to stop stirring, and drains the liquid from the sterilization tank.

9. An electronic device, characterized in that, include: processor; Memory used to store processor-executable instructions; The processor implements the method as described in any one of claims 1 to 6 by executing the executable instructions.

10. A computer-readable storage medium having a computer program stored thereon, characterized in that, When the program is executed by a processor, it implements the steps of the method as described in any one of claims 1 to 6.

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