Immunochromatographic device and detection method for detecting carbapenemase in bacteria

Abstract

The present invention relates to the field of microbial detection and discloses an immunochromatographic device and a detection method for detecting carbapenemase in bacteria. The immunochromatographic device includes a sample processing bag and an immunochromatographic box. The processing bag is divided into a lysis processing area, an adsorption processing area and a release processing area. The lysis processing area, the adsorption processing area and the release processing area are connected in sequence through a rupturable adhesive portion. The lysis processing area is filled with a lysis reagent for dissolving bacterial cell walls, the adsorption processing area is installed with resin particles for adsorbing carbapenemase, and the release processing area is arranged with a regulating reagent for regulating the release of carbapenemase from the resin. The immunochromatographic device and the detection method for detecting carbapenemase in bacteria proposed by the present invention can realize lysis, adsorption purification and release processing of bacteria in the sample solution, effectively improve the accuracy of the detection results, and are simple and convenient to operate.

Description

Technical Field

[0001] The present invention relates to the field of microbial detection, and more particularly to an immunochromatographic device and a detection method for detecting carbapenemase in bacteria. Background Art

[0002] Carbapenemases are enzymes that hydrolyze carbapenem antibiotics, which are important drugs used to treat a wide range of bacterial infections. Intrabacterial carbapenemases, which are present within bacterial cells, can reduce the efficacy of carbapenem antibiotics, leading to increased bacterial resistance to these drugs. This makes treating infections more difficult and can lead to antibiotic failure and continued disease spread. Therefore, research on intrabacterial carbapenemases and the development of new antibiotics are crucial.

[0003] Immunochromatographic devices are a common rapid test method used to detect the presence of a target substance. They are often used in in vitro diagnostics, such as for the detection of pathogens, drugs, hormones, and proteins.

[0004] The current immunochromatographic method for detecting carbapenemases generally involves lysing the bacteria in the sample, exposing the carbapenemases inside, and then detecting them using an immunochromatographic device. However, this method currently produces inaccurate test results. The main reasons are: the sample contains a small amount of specific bacteria, and after bacterial lysis, the amount of carbapenemases is too small, resulting in poor detection results; in addition, the sample contains other contaminating bacteria, which contain interfering proteins, resulting in poor detection results. These problems need to be solved. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention provides an immunochromatographic device and a detection method for detecting carbapenemases in bacteria.

[0006] According to one aspect of the present invention, an immunochromatographic device for detecting carbapenemases in bacteria is provided, comprising a sample processing bag and an immunochromatographic cartridge. The processing bag is divided into a lysis processing zone, an adsorption processing zone, and a release processing zone, wherein the lysis processing zone, the adsorption processing zone, and the release processing zone are sequentially connected via a rupturable adhesive portion. The lysis processing zone is filled with a lysis reagent for dissolving bacterial cell walls, the adsorption processing zone is installed with resin particles for adsorbing carbapenemases, and the release processing zone is arranged with a regulating reagent for regulating the release of carbapenemases from the resin.

[0007] Preferably: the release treatment zone and the cracking treatment zone are located in the upper half of the treatment bag, distributed left and right, a dropper is installed at the upper end of the release treatment zone, a sealing cover is installed at the upper end of the cracking treatment zone, and the adsorption treatment zone is located in the lower half of the treatment bag.

[0008] Preferably: a filter is installed in the dropper.

[0009] Preferably, a layer strip is arranged in the middle of the adsorption treatment area along the length direction of the treatment bag, and the layer strip separates the upper half of the adsorption treatment area.

[0010] Preferably, the immunochromatographic box comprises a reagent kit, a test strip is arranged in the reagent kit, a dripping area and an observation area are opened on the upper side of the reagent kit, and a control line is drawn on the side of the observation area.

[0011] A method for detecting carbapenemases in bacteria using an immunochromatographic device, comprising the following steps:

[0012] S1: First, the sample is discharged into the lysis treatment area and sealed with a lid. The lysis reagent in the lysis treatment area lyses the cell walls of the bacteria in the sample, exposing the carbapenemase in the bacteria.

[0013] S2: Then, the cleavage treatment zone is squeezed, squeezing the bonding portion between the cleavage treatment zone and the adsorption treatment zone, causing the solution to flow from the cleavage treatment zone into the adsorption treatment zone and contact the adsorption resin, which adsorbs the carbapenemase and part of the solution;

[0014] S3: Then, manually squeeze the adsorption treatment area to squeeze the resin particles in the adsorption treatment area toward the release treatment area, so that the resin particles are separated from the solution in the adsorption treatment area, and the bonding part between the adsorption treatment area and the release treatment area is broken, and the resin particles are squeezed into the release treatment area;

[0015] S4: Then, the resin particles come into contact with the regulating reagent in the release treatment area to release the carbapenemase, and then the release treatment area is squeezed to squeeze the resin particles toward the dropper, and the released carbapenemase and solution are squeezed out of the dropper and dripped into the dripping area of ​​the reagent kit;

[0016] S5: Observe whether the test strip is colored at the control line of the test kit to detect whether the sample contains carbapenemase.

[0017] Preferably, in step S2, manual operation is adopted, with one hand holding the sealing cover upwards to arrange the processing bag in an upright position, and the other hand continuously kneading and releasing the adsorption processing area to drive the resin particles inside to continuously flow, thereby improving the adsorption efficiency of the resin.

[0018] Preferably: in step S3, manual operation is adopted to keep the processing bag in an upright state, and the resin particles are squeezed to the position corresponding to the side of the release processing area, and then squeezed upward to make the resin particles separate from the solution on the lower side. Then, the resin particles are squeezed upward again to break the bonding part between the adsorption processing area and the release processing area, and the resin particles are squeezed toward the release processing area.

[0019] Preferably, the regulating agent is a powdered agent.

[0020] Preferably: in step S4, first keep the processing bag in an upright position, then pinch the bonding part between the adsorption processing area and the release processing area with one hand, and repeatedly knead and release the resin particles with the other hand to make the resin particles fully contact with the adjustment reagent, then, bend the processing bag so that the dropper is facing downward, and then squeeze the resin particles downward to squeeze the solution released in the resin particles out of the dropper.

[0021] The beneficial effects of the present invention are as follows: the immunochromatographic device and detection method for detecting carbapenemases in bacteria proposed in the present invention can realize the lysis, adsorption purification and release treatment of bacteria in the sample solution, effectively improving the accuracy of the detection results, and the operation is simple and convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Schematic diagram of a processing bag in an immunochromatographic device for detecting carbapenemases in bacteria proposed by the present invention;

[0023] Figure 2 Schematic diagram of the interior of a processing bag in an immunochromatographic device for detecting carbapenemases in bacteria proposed by the present invention;

[0024] Figure 3 Schematic diagram of a dropper in a processing bag in an immunochromatographic device for detecting carbapenemases in bacteria proposed by the present invention;

[0025] Figure 4 The present invention is a schematic diagram of an immunochromatographic cassette in an immunochromatographic device for detecting carbapenemases in bacteria.

[0026] In the figure: 100, processing bag; 110, sealing cap; 120, dropper; 121, filter; 130, cracking treatment area; 140, adsorption treatment area; 141, resin particles; 142, pressure strip; 150, release treatment area; 160, bonding part; 200, reagent box; 210, dripping area; 220, test strip; 230, observation area. DETAILED DESCRIPTION

[0027] The subject matter described herein will now be discussed with reference to example embodiments. It should be understood that these embodiments are discussed to enable those skilled in the art to better understand and implement the subject matter described herein. The functions and arrangements of the elements discussed may be varied without departing from the scope of protection of this specification. Various examples may omit, substitute, or add various processes or components as needed. In addition, features described with respect to some examples may also be combined in other examples.

[0028] Example 1

[0029] Reference Attachment Figures 1-4 In this embodiment, an immunochromatographic device for detecting carbapenemases in bacteria is proposed, including a processing bag 100 and an immunochromatographic cartridge. The processing bag 100 is divided into a lysis treatment zone 130, an adsorption treatment zone 140, and a release treatment zone 150. The lysis treatment zone 130, the adsorption treatment zone 140, and the release treatment zone 150 are sequentially connected through a rupturable adhesive portion 160. The lysis treatment zone 130 is filled with a lysis reagent for dissolving bacterial cell walls, the adsorption treatment zone 140 is installed with resin particles 141 for adsorbing carbapenemases, and the release treatment zone 150 is arranged with a regulating reagent for regulating the release of carbapenemases from the resin particles 141.

[0030] The release treatment area 150 and the cracking treatment area 130 are located in the upper half of the treatment bag 100, and are distributed on the left and right. A dropper 120 is installed at the upper end of the release treatment area 150, and a sealing cover 110 is installed at the upper end of the cracking treatment area 130. The adsorption treatment area 140 is located in the lower half of the treatment bag.

[0031] A filter screen 121 is installed in the dropper 120 .

[0032] A pressure strip 142 is arranged in the middle of the adsorption processing area 140 along the length direction of the processing bag 100 , and the pressure strip 142 separates the upper half of the adsorption processing area 140 .

[0033] The immunochromatographic box includes a test kit 200 , in which a test strip 220 is arranged. A dripping area 210 and an observation area 230 are opened on the upper side of the test kit 200 , and a control line is drawn on the side of the observation area 230 .

[0034] Example 2

[0035] This embodiment proposes a method for detecting carbapenemases in bacteria using the immunochromatographic device of Example 1, comprising the following steps:

[0036] S1: First, the sample is discharged into the lysis treatment area 130 and the sealing cover 110 is closed. The lysis reagent in the lysis treatment area 130 lyses the cell walls of the bacteria in the sample, exposing the carbapenemase in the bacteria.

[0037] S2: Then, the cracking treatment zone 130 is squeezed, and the bonding portion 160 between the cracking treatment zone 130 and the adsorption treatment zone 140 is squeezed, and the solution flows from the cracking treatment zone 130 into the adsorption treatment zone 140 and contacts the resin particles. The resin particles adsorb the carbapenemase and part of the solution.

[0038] S3: Then, the adsorption treatment area 140 is manually squeezed to push the resin particles 141 in the adsorption treatment area 140 toward the release treatment area 150, and the resin particles 141 are separated from the solution in the adsorption treatment area 140, and the bonding portion 160 between the adsorption treatment area 140 and the release treatment area 150 is broken, and the resin particles 141 are squeezed into the release treatment area 150;

[0039] S4: Then, the resin particles 141 contact the regulating reagent in the release treatment area 150 to release the carbapenemase, and then the release treatment area 150 is squeezed to squeeze the resin particles 141 toward the dropper 120. By squeezing, the released carbapenemase and solution are squeezed out of the dropper 120 and dripped into the dripping area 210 of the reagent kit 200;

[0040] S5: Observe whether the test strip 220 at the control line of the reagent kit 200 is colored, thereby detecting whether the sample contains carbapenemase.

[0041] In step S2, manual operation is adopted. One hand holds the sealing cover 110 upwards and arranges the processing bag 100 upright. The other hand continuously squeezes and releases the adsorption processing area 140 to drive the resin particles 141 therein to flow continuously, thereby improving the adsorption efficiency of the resin.

[0042] In step S3, manual operation is used to keep the processing bag 100 in an upright position, and the resin particles 141 are squeezed to the position corresponding to the side of the release processing area 150, and then squeezed upward to make the resin particles 141 separate from the solution on the lower side. Then, the resin particles 141 are squeezed upward again to break the bonding part 160 between the adsorption processing area 140 and the release processing area 150, and squeeze the resin particles 141 toward the release processing area 150.

[0043] The conditioning reagent is a powdered reagent.

[0044] In step S4, the processing bag 100 is first kept in an upright position, and then one hand pinches the bonding portion 160 between the adsorption processing area 140 and the release processing area 150, and the other hand repeatedly squeezes and releases the resin particles 141 so that the resin particles 141 are in full contact with the adjustment reagent. Then, the processing bag 100 is bent so that the dropper 120 is facing downward, and then the resin particles 141 are squeezed downward to squeeze the solution released in the resin particles 141 out of the dropper 120.

[0045] The immunochromatographic device and detection method for detecting carbapenemases in bacteria proposed in the present invention can achieve lysis, adsorption purification and release treatment of bacteria in the sample solution, effectively improving the accuracy of the detection results and being simple and convenient to operate.

[0046] The embodiment of the present embodiment is described above in conjunction with the accompanying drawings, but the present embodiment is not limited to the above-mentioned specific implementation methods. The above-mentioned specific implementation methods are merely illustrative and not restrictive. Under the guidance of this embodiment, ordinary technicians in this field can also make many forms without departing from the purpose of this embodiment and the scope of protection of the claims, all of which are protected by this embodiment.

Claims

1. An immunochromatographic device for detecting carbapenemases in bacteria, characterized in that: The invention comprises a processing bag (100) and an immunochromatographic box, wherein the processing bag (100) is divided into a lysis processing area (130), an adsorption processing area (140) and a release processing area (150), wherein the lysis processing area (130), the adsorption processing area (140) and the release processing area (150) are sequentially connected through a rupturable adhesive portion (160), wherein the lysis processing area (130) is filled with a lysis reagent for dissolving bacterial cell walls, wherein the adsorption processing area (140) is provided with resin particles (141) for adsorbing carbapenemase, and wherein the release processing area (150) is provided with a regulating reagent for regulating the release of carbapenemase from the resin particles (141); The release treatment zone (150) and the cracking treatment zone (130) are located in the upper half of the treatment bag (100), and are distributed on the left and right. A dropper (120) is installed at the upper end of the release treatment zone (150), and a sealing cover (110) is installed at the upper end of the cracking treatment zone (130). The adsorption treatment zone (140) is located in the lower half of the treatment bag. A filter screen (121) is installed in the dropper (120); A layering strip (142) is arranged in the middle of the adsorption processing area (140) along the length direction of the processing bag (100), and the layering strip (142) separates the upper half of the adsorption processing area (140); The immunochromatographic box comprises a test kit (200), wherein a test strip (220) is arranged in the test kit (200), a dripping area (210) and an observation area (230) are opened on the upper side of the test kit (200), and a control line is drawn on the side of the observation area (230); The method for detecting carbapenemases in bacteria using an immunochromatographic device comprises the following steps: S1: First, the sample is discharged into the lysis treatment area (130), and the sealing cover (110) is closed. The lysis reagent in the lysis treatment area (130) lyses the cell walls of the bacteria in the sample, thereby exposing the carbapenemase in the bacteria; S2: Then, the cracking treatment zone (130) is squeezed, and the bonding portion (160) between the cracking treatment zone (130) and the adsorption treatment zone (140) is squeezed, and the solution flows from the cracking treatment zone (130) into the adsorption treatment zone (140) and contacts the resin particles, and the resin particles adsorb the carbapenemase and part of the solution; S3: Then, manually squeeze the adsorption treatment area (140) to squeeze the resin particles (141) in the adsorption treatment area (140) toward the release treatment area (150), and make the resin particles (141) separate from the solution in the adsorption treatment area (140), squeeze the bonding part (160) between the adsorption treatment area (140) and the release treatment area (150), and squeeze the resin particles (141) into the release treatment area (150); S4: Then, the resin particles (141) contact the regulating reagent in the release processing area (150) to release the carbapenemase, and then the release processing area (150) is squeezed to squeeze the resin particles (141) toward the dropper (120), and the released carbapenemase solution is squeezed out of the dropper (120) and dripped into the dripping area (210) of the reagent kit (200); S5: Observe whether the test strip (220) at the control line of the reagent kit (200) is colored, thereby detecting whether the sample contains carbapenemase; In step S2, manual operation is adopted, with one hand holding the sealing cover (110) upwards, arranging the processing bag (100) in a vertical position, and the other hand continuously kneading and releasing the adsorption processing area (140) to drive the resin particles (141) therein to continuously flow, thereby improving the adsorption efficiency of the resin; In step S3, manually operating the processing bag (100) while keeping it in an upright position, the resin particles (141) are squeezed to a position corresponding to one side of the release processing area (150), and then squeezed upwards so that the resin particles (141) are separated from the solution located on the lower side. Then, the resin particles (141) are squeezed upwards again to break the bonding portion (160) between the adsorption processing area (140) and the release processing area (150), and the resin particles (141) are squeezed toward the release processing area (150); The regulating reagent is a powdered reagent; In step S4, the processing bag (100) is first kept in an upright position, and then one hand pinches the bonding portion (160) between the adsorption processing area (140) and the release processing area (150), and the other hand repeatedly squeezes and releases the resin particles (141) so that the resin particles (141) are fully in contact with the adjustment reagent. Then, the processing bag (100) is bent so that the dropper (120) is facing downward, and then the resin particles (141) are squeezed downward to squeeze the solution released in the resin particles (141) out of the dropper (120).

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