A hericium erinaceus composition against helicobacter pylori and a preparation method thereof

Abstract

The present application relates to the technical field of health products, in particular to a monkey head mushroom composition for resisting helicobacter pylori and a preparation method thereof, the monkey head mushroom composition is prepared from the following components in parts by weight: 20-35 parts of monkey head mushroom, 10-20 parts of dandelion, 20-30 parts of grifola frondosa, 20-30 parts of tender gossypium hirsutum pods, 10-15 parts of gardenia, 10-15 parts of gordon euryale seed, 5-10 parts of cumin, and 25.6-51.3 parts of a blending agent; wherein the blending agent is compounded from soybean oligopeptide, L-isoleucine, glutamine and erythritol. In the present application, dandelion, gardenia and cumin are combined, and monkey head mushroom, grifola frondosa, gordon euryale seed, soybean oligopeptide, L-isoleucine, glutamine and erythritol are combined, so that the stomach and intestinal mucosa damage can be quickly repaired and the stomach function can be strengthened.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of health products, in particular to a Hericium erinaceum composition for resisting Helicobacter pylori and a preparation method thereof. BACKGROUND

[0002] Helicobacter pylori is a Gram-negative bacterium that colonizes the stomach, and about 10% of infected people will develop chronic atrophic gastritis, functional dyspepsia and digestive tract ulcers, which are closely related to diseases such as gastric cancer and gastric mucosa-associated lymphoid tissue lymphoma, and is listed as the first class of biological carcinogens by the World Health Organization.

[0003] Currently, oral combined antibiotic preparations are basically used for treatment in the clinic, which has a large side effect and is extremely easy to cause patients to have symptoms such as nausea, vomiting, abdominal distension and constipation, and the application range of antibiotics is limited, such as pregnant women, children, the elderly, antibiotic allergy patients and patients with liver and kidney dysfunction, who are not suitable for antibiotic therapy.

[0004] Hericium erinaceum is a precious edible fungus, which has the functions of promoting qi and digestion, invigorating the spleen and appetite, and soothing the nerves and intelligence. Studies have shown that the active ingredients such as polypeptides, polysaccharides, fats and proteins contained in Hericium erinaceum have certain effects on digestive tract tumors, gastric and duodenal ulcers, gastritis and abdominal distension.

[0005] At present, there is a lack of health food that can not only kill Helicobacter pylori but also protect the gastric mucosa from damage in the market. In view of this, we propose a Hericium erinaceum composition for resisting Helicobacter pylori and a preparation process. SUMMARY

[0006] To solve the above problems, the present application provides a Hericium erinaceum composition for resisting Helicobacter pylori and a preparation method thereof.

[0007] To achieve the above-mentioned purposes, the technical scheme adopted by the present application is as follows:

[0008] A Hericium erinaceum composition for resisting Helicobacter pylori is prepared from the following components in parts by weight:

[0009] Hericium erinaceum 20-35 parts, dandelion 10-20 parts, Grifola frondosa 20-30 parts, tender gourd 20-30 parts, gardenia 10-15 parts, Euryale 10-15 parts, cumin 5-10 parts, and a blending agent 25.6-51.3 parts; wherein the blending agent is compounded from soybean oligopeptide, L-isoleucine, glutamine and erythritol.

[0010] Further, the blending agent includes, in parts by weight:

[0011] soybean oligopeptide 5-10 parts, L-isoleucine 0.1-0.3 parts, glutamine 0.5-1 part, and erythritol 20-40 parts.

[0012] Preferably, the obtained product is prepared from the following components by weight ratio:

[0013] Hou tou gu 20 parts, Pugongying 10 parts, Hui shan gu 20 parts, Yellow okra tender pod 20 parts, Shan zi zi 10 parts, Gorgon 10 parts, Fennel 5 parts, Soybean oligopeptide 5 parts, L-isoleucine 0.1 parts, Glutamine 0.5 parts, Erythritol 20 parts.

[0014] Preferably, the obtained product is prepared from the following components by weight ratio:

[0015] Hou tou gu 20 parts, Pugongying 10 parts, Hui shan gu 20 parts, Yellow okra tender pod 20 parts, Shan zi zi 10 parts, Gorgon 10 parts, Fennel 5 parts, Soybean oligopeptide 5 parts, L-isoleucine 0.1 parts, Glutamine 0.5 parts, Erythritol 20 parts.

[0016] Preferably, the obtained product is prepared from the following components by weight ratio:

[0017] Hou tou gu 20 parts, Pugongying 10 parts, Hui shan gu 20 parts, Yellow okra tender pod 20 parts, Shan zi zi 10 parts, Gorgon 10 parts, Fennel 5 parts, Soybean oligopeptide 5 parts, L-isoleucine 0.1 parts, Glutamine 0.5 parts, Erythritol 20 parts.

[0018] The application also provides a method for preparing the above-mentioned Hou tou gu composition, comprising the following steps:

[0019] S1, take the formula amount of Hou tou gu (dry), Hui shan gu (dry) and Gorgon, respectively grind to 200 mesh, and mix and stir the Hou tou gu powder and Hui shan gu powder uniformly to obtain Hou tou gu-Hui shan gu powder and Gorgon powder;

[0020] S2, take the formula amount of Pugongying, Shan zi zi and Fennel, after crushing, place them in an ultrasonic extractor, add appropriate amount of purified water according to the solid-liquid ratio of 1:20, ultrasonic extraction under the condition of 60-70℃ and power of 350-400w for 30-40min, filter, reserve the filtrate, take the residue, place it in an enzymatic hydrolysis tank, add appropriate amount of purified water according to the solid-liquid ratio of 1:10, add appropriate amount of plant compound hydrolytic enzyme according to the proportion of 5%-6%, and enzymatically hydrolyze at 40-50℃ for 2-3h, filter, and combine the two filtrates to obtain the extraction liquid;

[0021] S3, take the formula amount of Yellow okra tender pod, cut it into pieces, and place it in a high-pressure tank, use saturated water vapor as medium, maintain the pressure at 0.6-1.0Mpa for 120-150s, quickly restore to normal pressure, dry, and obtain Yellow okra tender pod powder;

[0022] S4, taking the okra tender pod powder with acid water heat extraction, centrifugal separation, okra tender pod residue and supernatant were obtained, ethanol was added to the supernatant to precipitate, and the precipitate was obtained by centrifugal separation to obtain okra pectin;

[0023] S5, taking the okra tender pod residue, and placing it in an enzyme hydrolysis tank together with gordon euryale seed powder, adding appropriate amount of purified water and neutral protease, hydrolyzing under the conditions of pH 8.0, temperature 45 DEG C, substrate concentration 5%, enzyme amount 4000 U / g for 3h, then using alkaline protease under the conditions of pH 8.5, temperature 50 DEG C, substrate concentration 3%, enzyme amount 5000 U / g for 3h, and spray drying to obtain enzyme hydrolysis powder;

[0024] S6, the obtained Hericium erinaceus-Hypholoma capnoides powder, extract and enzyme hydrolysis powder were mixed and stirred uniformly, concentrated, granulated, dried, and once sieved to obtain pre-made microparticles (24-30 mesh);

[0025] S7, dissolving the formula amount of soybean oligopeptide, L-isoleucine, glutamine and erythritol in 3 times the amount of water, and then uniformly mixing with the obtained okra pectin to obtain a coating liquid;

[0026] S8, preheating the pre-made microparticles at 40 DEG C for 3 min, placing them in a fluidized bed coating machine, using bottom spraying, spraying liquid speed is 4.0-5.0 mL / min, air inlet frequency is 30-40 Hz, the temperature in the fluidized bed coating machine is always maintained at 30-40 DEG C, after coating, the coated microparticles continue to be dried in the fluidized bed to a water content of about 1%, and then taken out.

[0027] Further, in step S4, the okra tender pod powder is mixed with hydrochloric acid with pH 2-5 at a ratio of 1g:10-20mL, and stirred and extracted at 50-80 DEG C for 50-120 min; the centrifugal separation rate is 5000-7000 r / min, and the centrifugal time is 20-40 min; the solid is extracted by acid water heat extraction, then centrifuged and separated, and the supernatants of the two times are combined.

[0028] Further, in step S4, the ethanol is 75-95v% ethanol, and the volume ratio of ethanol to supernatant is 1-3:1; the centrifugal separation rate is 5000-7000 r / min, and the centrifugal time is 20-40 min; the precipitate after centrifugal separation is washed 2-3 times with 40-50v% ethanol.

[0029] In the present application, dandelion, gardenia and fennel are used as main sterilization preparations, wherein:

[0030] Herba Taraxaci: It can "tonify the spleen and stomach, and purge fire" according to the Record of the Medical School, and is used to treat stomach and abdominal diseases, one for clearing stomach fire and one for resisting bacteria and eliminating inflammation. Modern medicine has proved that some effective components contained in Herba Taraxaci can be used to treat stomach and abdominal pain and have a significant effect, and common symptoms such as stomach and abdominal pain, fullness, vomiting and acid regurgitation caused by food stagnation and heat, gastrointestinal dampness caused by overeating and drinking, and stomach caused by liver depression and fire can be treated by Herba Taraxaci. In addition, Herba Taraxaci leaves also contain effective components that can inhibit the growth of Salmonella typhi, Pseudomonas aeruginosa and Staphylococcus aureus, and the effect of killing Helicobacter pylori is also supported by clinical data.

[0031] Fructus Gardeniae: It has a broad-spectrum antibacterial effect, and can inhibit Pseudomonas aeruginosa, Staphylococcus aureus and various skin pathogenic fungi. It also has the functions of detoxification, anti-inflammatory and detumescence.

[0032] Foeniculum vulgare: Modern research shows that Foeniculum vulgare contains effective components such as fatty oil, volatile oil, sterol, glycoside and alkaloid, and has the effects of anti-inflammatory analgesia, antibiosis, increasing gastrointestinal peristalsis and the like.

[0033] In the present application, Hericium erinaceus can inhibit the growth of Helicobacter pylori and reduce its damage to gastric mucosa, Grifola frondosa can enhance the immunity of the body and promote the recovery and repair of the inflammation site, Euryale ferox has multiple pharmacological effects such as antioxidant, anti-fatigue and immunity improvement, it can promote intestinal peristalsis and improve gastric cells at the same time, soybean oligopeptide can reduce the expression of inflammatory factors and improve the immunity of the body, L-isoleucine can affect the activity and function of immune cells, promote the production of antibodies and cellular immune response, glutamine has the effects of protection and repair on gastric and intestinal mucosal damage, and is beneficial to the recovery of intestinal absorption function and immune function of the body, the five components can jointly and synergistically quickly repair gastric and intestinal mucosal damage and strengthen the function of the stomach.

[0034] In the present application, the rich pectin, soluble dietary fiber and vitamin C contained in the tender pods of Okra can well help gastrointestinal peristalsis, can be dissolved in a gastric acid environment to form a mucus that protects the gastric mucosa, and in turn protects the gastric wall, on the one hand, it can strengthen the repair effects of Hericium erinaceus and glutamine, and on the other hand, it can exist as an adhesive and a coating agent, and can well ensure the uniformity, delicacy and taste of the obtained microparticles. BRIEF DESCRIPTION OF DRAWINGS

[0035] Figure 1 A schematic diagram of the bactericidal effect of each composition in the embodiments of the present application on HP is shown. DETAILED DESCRIPTION

[0036] The application will be described in detail below with specific examples. The following examples will help those skilled in the art to further understand the application, but do not limit the application in any form. It should be pointed out that those skilled in the art can make several modifications and improvements without departing from the concept of the application. These are all within the scope of protection of the application.

[0037] Example 1

[0038] A Hericium erinaceus composition against Helicobacter pylori is prepared from the following components in parts by weight:

[0039] Hericium erinaceus 20 parts, Taraxacum mongolicum 10 parts, Grifola frondosa 20 parts, tender gourd of Okra 20 parts, Gardenia jasminoides 10 parts, Euryale ferox 10 parts, Foeniculum vulgare 5 parts, soybean oligopeptide 5 parts, L-isoleucine 0.1 part, glutamine 0.5 part, erythritol 20 parts.

[0040] In preparation, the following steps are included:

[0041] S1, take the prescription amount of Hericium erinaceus (dry), Grifola frondosa (dry), Euryale ferox, grind to 200 mesh respectively, and mix and stir the Hericium erinaceus powder and Grifola frondosa powder uniformly to obtain Hericium erinaceus-Grifola frondosa powder and Euryale ferox powder;

[0042] S2, take the prescription amount of Taraxacum mongolicum, Gardenia jasminoides, Foeniculum vulgare, crush and treat, then place in an ultrasonic extractor, add appropriate amount of purified water at a solid-liquid ratio of 1:20, ultrasonic extraction at 60℃ and a power of 350w for 40min, filter, reserve the filtrate, take the residue, place in an enzymatic hydrolysis tank, add appropriate amount of purified water at a solid-liquid ratio of 1:10, add appropriate amount of plant compound hydrolytic enzyme Viscozyme L at a proportion of 5%, enzymatic hydrolysis at 40℃ for 3h, filter, combine the two filtrates to obtain the extract;

[0043] S3, take the prescription amount of tender gourd of Okra, cut into pieces, place in a high-pressure tank, use saturated water vapor as medium, maintain pressure at 0.6Mpa for 150s, quickly restore to normal pressure, dry to obtain tender gourd of Okra powder;

[0044] S4, mix the tender gourd of Okra powder with hydrochloric acid at pH 2 at a solid-liquid ratio of 1g:10mL, stir and extract at 80℃ for 50min; centrifugal separation at a rate of 6000r / min for 30min; the solid is extracted by acid hydrothermal extraction method, then centrifugally separated, and the two supernatants are combined to obtain tender gourd of Okra residue and supernatant, add 75v% ethanol to the supernatant for precipitation, the volume ratio of ethanol to supernatant is 3:1; centrifugal separation at a rate of 6000r / min for 30min; the precipitate after centrifugal separation is washed twice with 45v% ethanol to obtain Okra pectin;

[0045] S5, the tender gourd residue is taken and placed in an enzymolysis tank together with the Euryale ferox powder, and then purified water and neutral protease are added, and hydrolysis is carried out under the conditions of pH 8.0, temperature 45℃, substrate concentration 5%, and enzyme amount 4000 U / g for 3 h, and then alkaline protease is used to hydrolyze under the conditions of pH 8.5, temperature 50℃, substrate concentration 3%, and enzyme amount 5000 U / g for 3 h, and then spray drying is performed to obtain the enzymolysis powder;

[0046] S6, the obtained Hericium erinaceum - Grifola frondosa powder, extract, and enzymolysis powder are mixed and stirred uniformly, concentrated, granulated, and dried to obtain pre-made microparticles (24-30 mesh) by primary screening;

[0047] S7, the formula amount of soybean oligopeptide, L-isoleucine, glutamine, and erythritol is dissolved in 3 times the amount of water, and then mixed uniformly with the obtained gourd pectin to obtain a coating liquid;

[0048] S8, the pre-made microparticles are preheated at 40℃ for 3 min, placed in a fluidized bed coating machine, and coated by using a bottom spraying type with a spraying speed of 4.5 mL / min and an air inlet frequency of 35 Hz, and the temperature in the fluidized bed coating machine is maintained at 35℃ at all times, and after the coating is completed, the coated microparticles continue to be dried in the fluidized bed until the water content is about 1% and then taken out.

[0049] Example 2

[0050] A Hericium erinaceum composition for resisting Helicobacter pylori is prepared from the following components in parts by weight:

[0051] Hericium erinaceum 35 parts, dandelion 20 parts, Grifola frondosa 30 parts, tender gourd 30 parts, Fructus gardeniae 15 parts, Euryale ferox 15 parts, cumin 10 parts, soybean oligopeptide 10 parts, L-isoleucine 0.3 parts, glutamine 1 part, and erythritol 40 parts.

[0052] In the preparation, the following steps are included:

[0053] S1, the formula amount of Hericium erinaceum (dry), Grifola frondosa (dry), and Euryale ferox is ground to 200 mesh, and the Hericium erinaceum powder and Grifola frondosa powder are mixed and stirred uniformly to obtain Hericium erinaceum - Grifola frondosa powder and Euryale ferox powder;

[0054] S2, the formula amount of dandelion, Fructus gardeniae, and cumin is crushed and treated, and then placed in an ultrasonic extractor, and then appropriate purified water is added according to a solid-liquid ratio of 1:20, and ultrasonic extraction is carried out under the conditions of 70℃ and power 400 w for 30 min, and then filtered, and the filtrate is reserved for use, and the filter residue is placed in an enzymolysis tank, and then appropriate purified water is added according to a solid-liquid ratio of 1:10, and then appropriate plant compound hydrolysis enzyme Viscozyme L is added according to a proportion of 6%, and then enzymolysis is carried out at 50℃ for 2 h, and then filtered, and the filtrates of the two times are combined to obtain an extract;

[0055] S3, cut the formula amount of okra tender pod into pieces, and then put into a high-pressure tank, and use saturated water vapor as medium, and maintain pressure at 1.0 Mpa for 120 seconds, and then quickly restore to normal pressure, and dry, and obtain okra tender pod powder;

[0056] S4, mix the okra tender pod powder with hydrochloric acid with pH of 5 according to the ratio of 1g:20mL, and stir and soak at 50℃ for 120 minutes; centrifugal separation rate is 6000r / min, and centrifugal time is 30 minutes; the solid is extracted by acid hydrothermal extraction method, and then centrifuged and separated, and the two supernatants are combined, and obtain okra tender pod residue and supernatant, and add 95v% ethanol to the supernatant for precipitation, and the volume ratio of ethanol to supernatant is 1:1; centrifugal separation rate is 6000r / min, and centrifugal time is 30 minutes; the precipitate after centrifugal separation is washed twice with 40v% ethanol, and obtain okra pectin;

[0057] S5, take the okra tender pod residue, and put it into an enzymatic hydrolysis tank together with euryale seed powder, and add appropriate amount of purified water and neutral protease, and hydrolyze under the conditions of pH 8.0, temperature 45℃, substrate concentration 5%, and enzyme amount 4000U / g for 3 hours, and then hydrolyze with alkaline protease under the conditions of pH 8.5, temperature 50℃, substrate concentration 3%, and enzyme amount 5000U / g for 3 hours, and spray dry to obtain enzymatic hydrolysis powder;

[0058] S6, mix the obtained Hericium erinaceus-grifola frondosa powder, extract, and enzymatic hydrolysis powder uniformly, and then concentrate, granulate, and dry, and once sieve to obtain pre-made microparticles (24-30 mesh);

[0059] S7, dissolve the formula amount of soybean oligopeptide, L-isoleucine, glutamine, and erythritol in 3 times amount of water, and then mix uniformly with the obtained okra pectin by ultrasonic to obtain coating liquid;

[0060] S8, preheat the pre-made microparticles at 40℃ for 3 minutes, and then put into a fluidized bed coating machine, and use bottom spraying type, and the spraying speed is 4.5mL / min, and the air inlet frequency is 35Hz, and the temperature in the fluidized bed coating machine is maintained at 35℃ at all times, and after coating, the coated microparticles continue to be dried in the fluidized bed until the water content is about 1%, and then take out, and obtain the product.

[0061] Example 3

[0062] A Hericium erinaceus composition for resisting Helicobacter pylori is prepared from the following components by weight fraction:

[0063] Hericium erinaceus 17.5 parts, dandelion 15 parts, grifola frondosa 25 parts, okra tender pod 25 parts, gardenia 12.5 parts, euryale seed 12.5 parts, cumin 7.5 parts, soybean oligopeptide 7.5 parts, L-isoleucine 0.2 parts, glutamine 0.75 parts, and erythritol 30 parts.

[0064] When preparing, the following steps are included:

[0065] S1, take the formula amount of Hericium erinaceus (dry), Grifola frondosa (dry), Euryale ferox, grind to 200 mesh respectively, and mix and stir the Hericium erinaceus powder and Grifola frondosa powder uniformly to obtain Hericium erinaceus-Grifola frondosa powder and Euryale ferox powder;

[0066] S2, take the formula amount of dandelion, gardenia, and cumin, crush and treat, then put them into an ultrasonic extractor, add appropriate amount of purified water according to the solid-liquid ratio of 1:20, ultrasonic extraction under the condition of 65℃ and power 375w for 35min, filter, reserve the filtrate, take the residue, put it into an enzymatic hydrolysis tank, add appropriate amount of purified water according to the solid-liquid ratio of 1:10, add appropriate amount of plant compound hydrolytic enzyme Viscozyme L according to the proportion of 5.5%, enzymatic hydrolysis at 45℃ for 2.5h, filter, and combine the two filtrates to obtain the extract;

[0067] S3, take the formula amount of yellow okra tender pod, cut it into pieces, put it into a high-pressure tank, use saturated water vapor as medium, maintain the pressure at 0.8Mpa for 130s, quickly restore to normal pressure, dry to obtain yellow okra tender pod powder;

[0068] S4, mix the yellow okra tender pod powder with hydrochloric acid with pH 4 according to the solid-liquid ratio of 1g:15mL, stir and extract at 65℃ for 90min; centrifugal separation at the speed of 6000r / min for 30min; the solid is extracted by acid hydrothermal method, then centrifugally separated, and the two supernatants are combined to obtain yellow okra tender pod residue and supernatant, add 95v% ethanol to the supernatant for precipitation, the volume ratio of ethanol to supernatant is 1:1; centrifugal separation at the speed of 6000r / min for 30min; the precipitate after centrifugal separation is washed twice with 40v% ethanol to obtain yellow okra pectin;

[0069] S5, take the yellow okra tender pod residue, put it into an enzymatic hydrolysis tank together with Euryale ferox powder, add appropriate amount of purified water and neutral protease, hydrolyze at pH 8.0, temperature 45℃, substrate concentration 5%, enzyme amount 4000U / g for 3h, then hydrolyze with alkaline protease at pH 8.5, temperature 50℃, substrate concentration 3%, enzyme amount 5000U / g for 3h, spray dry to obtain enzymatic hydrolysis powder;

[0070] S6, mix and stir the obtained Hericium erinaceus-Grifola frondosa powder, extract, and enzymatic hydrolysis powder uniformly, concentrate, granulate, and dry to obtain pre-made microparticles (24-30 mesh) by sieving once;

[0071] S7, dissolve the formula amount of soybean oligopeptide, L-isoleucine, glutamine, and erythritol in 3 times amount of water, then mix and uniformly with the obtained yellow okra pectin to obtain a coating liquid;

[0072] S8, preheat the pre-made microparticles at 40℃ for 3 min, and place them in a fluidized bed coater. Use bottom spraying, with a liquid spraying speed of 4.5 mL / min and an air inlet frequency of 35 Hz. The temperature in the fluidized bed coater is maintained at 35℃ throughout. After coating, continue to dry the coated microparticles in the fluidized bed until the water content is about 1%, and then take them out.

[0073] Comparative Example 1

[0074] Replace the Fructus Gardeniae in Example 1 with an equal amount of Herba Taraxaci, and follow the same design as in Example 1.

[0075] Comparative Example 2

[0076] Replace the Fructus Foeniculi in Example 2 with an equal amount of Fructus Gardeniae, and follow the same design as in Example 2.

[0077] Comparative Example 3

[0078] Replace both the Fructus Gardeniae and the Fructus Foeniculi in Example 3 with an equal amount of Herba Taraxaci, and follow the same design as in Example 3.

[0079] Comparative Example 4

[0080] Omit the L-isoleucine in Example 3, and follow the same design as in Example 3.

[0081] Comparative Example 5

[0082] Omit the glutamine in Example 3, and follow the same design as in Example 3.

[0083] Comparative Example 6

[0084] S3, crush the prescribed amount of Abelmoschus esculentus tender pod to 100 mesh, dry, and obtain Abelmoschus esculentus tender pod powder;

[0085] Follow the same design as in Example 3

[0086] Calculate the extraction rate of Abelmoschus esculentus pectin in Example 3 and Comparative Example 6, respectively. The Abelmoschus esculentus pectin yield in Example 3 is 7.73%, while the Abelmoschus esculentus pectin yield in Comparative Example 6 is 4.372%. It can be seen that the method of placing Abelmoschus esculentus tender pod in a high-pressure tank and performing steam explosion treatment with saturated water vapor as the medium can significantly improve the yield of Abelmoschus esculentus pectin.

[0087] Comparative Example 7

[0088] Follow the same design as in Example 3, and include the following steps in the preparation:

[0089] S1, grind the prescribed amount of Hericium erinaceus (dry), Grifola frondosa (dry), and Euryale ferox into 200 mesh, and mix and stir the Hericium erinaceus powder and Grifola frondosa powder uniformly to obtain Hericium erinaceus-Grifola frondosa powder and Euryale ferox powder;

[0090] S2, take the amount of dandelion, mountain gardenia, fennel, after crushing, placed in the ultrasonic extractor, adding appropriate amount of purified water, 65℃, power 375w conditions, ultrasonic extraction 35min, filtration, filter for use, take the filter residue, placed in the enzyme hydrolysis tank, according to the ratio of 1∶10, adding appropriate amount of purified water, according to the proportion of 5.5% to add appropriate amount of plant composite hydrolytic enzyme Viscozyme L, 45℃ enzyme hydrolysis 2.5h, filtration, combined with two filtrates, obtained the extract;

[0091] S3, take the amount of yellow squash tender pod cut into pieces, placed in the high pressure tank, with saturated steam as medium, under the condition of 0.8Mpa, pressure 130s, quickly restore to normal pressure, drying, get yellow squash tender pod powder;

[0092] S4, the yellow squash tender pod powder and hydrochloric acid with pH 4 were mixed in the ratio of 1g∶15mL, stirring at 65℃ for 90min; the centrifugal separation rate was 6000r / min, and the centrifugal time was 30min; the solid was extracted by acid hydrothermal extraction, then centrifuged and separated, and the supernatant of the two times was combined to obtain the yellow squash tender pod residue and the supernatant. 95v% ethanol was added to the supernatant to precipitate, and the volume ratio of ethanol to supernatant was 1∶1; the centrifugal separation rate was 6000r / min, and the centrifugal time was 30min; the precipitate was washed twice with 40v% ethanol after centrifugal separation to obtain the yellow squash pectin;

[0093] S5, the obtained Hericium erinaceus-Hericium erinaceus powder, extract, yellow squash tender pod residue, Euryale ferox powder were mixed and stirred uniformly, concentrated, granulated, dried, and once sieved to obtain pre-made microparticles (24-30 mesh);

[0094] S6, the formula amount of soybean oligopeptide, L-isoleucine, glutamine and erythritol were dissolved in 3 times the amount of water, and then mixed with the obtained yellow squash pectin to obtain a coating liquid;

[0095] S7, the pre-made microparticles were preheated at 40℃ for 3min, placed in a fluidized bed coating machine, and a bottom spraying type was adopted with a liquid spraying speed of 4.5mL / min and an air inlet frequency of 35Hz. The temperature in the fluidized bed coating machine was maintained at 35℃ at all times. After coating, the coated microparticles were dried in the fluidized bed until the water content was about 1% and then taken out.

[0096] Comparative example 8

[0097] The formula was the same as that of example 3, and the preparation included the following steps:

[0098] S1, take the formula amount of Hericium erinaceus (dry), Grifola frondosa (dry), Euryale ferox, respectively, grind to 200 mesh, and mix and stir the Hericium erinaceus powder and Grifola frondosa powder uniformly to obtain Hericium erinaceus-Grifola frondosa powder and Euryale ferox powder;

[0099] S2, take the formula amount of dandelion, gardenia, cumin, after crushing, place in the ultrasonic extractor, add appropriate amount of purified water according to the solid-liquid ratio of 1:20, ultrasonic extraction at 65℃ and power of 375w for 35min, filter, filter the filtrate for use, take the filter residue, place in the enzyme hydrolysis tank, add appropriate amount of purified water according to the solid-liquid ratio of 1:10, add appropriate amount of plant compound hydrolytic enzyme Viscozyme L according to the proportion of 5.5%, enzyme hydrolysis at 45℃ for 2.5h, filter, combine the two filtrates to obtain the extract;

[0100] S3, take the formula amount of yellow squash tender pod, cut into pieces, place in the high-pressure tank, use saturated water vapor as medium, maintain pressure at 0.8Mpa for 130s, quickly restore to normal pressure, dry to obtain yellow squash tender pod powder;

[0101] S4, mix the yellow squash tender pod powder with hydrochloric acid with pH of 4 according to the solid-liquid ratio of 1g:15mL, stir and extract at 65℃ for 90min; centrifugal separation rate is 6000r / min, centrifugal time is 30min; the solid is extracted by acid hydrothermal extraction method, then centrifugally separated, and the two supernatants are combined to obtain yellow squash tender pod residue and supernatant, add 95v% ethanol to the supernatant for precipitation, the volume ratio of ethanol to supernatant is 1:1; centrifugal separation rate is 6000r / min, centrifugal time is 30min; the precipitate after centrifugal separation is washed twice with 40v% ethanol to obtain yellow squash pectin;

[0102] S5, take the yellow squash tender pod residue, place in the enzyme hydrolysis tank together with Euryale ferox powder, add appropriate amount of purified water and neutral protease, hydrolyze at pH8.0, temperature 45℃, substrate concentration 5%, enzyme amount 4000U / g for 3h, then hydrolyze with alkaline protease at pH8.5, temperature 50℃, substrate concentration 3%, enzyme amount 5000U / g for 3h, spray dry to obtain enzyme hydrolysis powder;

[0103] S6, take the formula amount of soybean oligopeptide, L-isoleucine, glutamine, erythritol, dissolve in 3 times amount of water, mix uniformly with the obtained yellow squash pectin, mix and stir uniformly with the obtained Hericium erinaceus-Grifola frondosa powder, extract, granulate, dry to about 1% water content, take out, sieve once to obtain pre-made microparticles (24-30 mesh).

[0104] (1) In vitro anti-Helicobacter pylori test

[0105] The activated (Hp11639) strain was taken in 10 mL liquid medium and cultured for 12-18 h to prepare a bacterial suspension, and the concentration of the bacterial suspension was adjusted to 1 x 10 8 CFU / mL. A certain amount of the composition obtained in Examples 1-3 and Comparative Examples 1-5 was added to the liquid medium to prepare a test sample liquid with a concentration of 50 / mL. 1 mL of the bacterial suspension was added to different test sample liquids, respectively, and a control tube without the composition was set. The test tubes and the control tube were simultaneously placed in a 36°C microaerobic environment (CO210%, O25%) for 24 h. Samples were taken from each tube, diluted appropriately, and spread on a BHI agar medium plate, which was incubated at 36°C in a microaerobic environment for 72 h. The number of surviving bacteria on the plate was counted. The number of bacteria on the plate of the control tube was counted as the initial number of bacteria. The bactericidal rate was calculated according to the formula: bactericidal rate = (initial number of bacteria - number of surviving bacteria) / initial number of bacteria x 100%. The results are shown in Table 1 and Figure 1 .

[0106] Table 1 Comparison of bactericidal effects of various compositions on HP

[0107] Group Bactericidal rate / 100% Example 1 99.5 Example 2 99.6 Example 3 99.8 Comparative Example 1 91.6 Comparative Example 2 94.8 Comparative Example 3 87.5 Comparative Example 4 95.3 Comparative Example 5 98.3

[0108] As can be seen from Table 1 and Figure 1 , the bactericidal rates of the examples were significantly higher than those of the control group. In Comparative Example 1, the Fructus Gardeniae in Example 1 was replaced with an equal amount of Taraxacum mongolicum, and the bactericidal rate was significantly lower than that of Example 1. In Comparative Example 2, the Cuminum cyminum in Example 2 was replaced with an equal amount of Fructus Gardeniae, and the bactericidal rate was significantly lower than that of Example 2. In Comparative Example 3, both Fructus Gardeniae and Cuminum cyminum in Example 3 were replaced with an equal amount of Taraxacum mongolicum, and the bactericidal rate was significantly lower than that of Example 3. It can be seen that there is a synergistic relationship among Taraxacum mongolicum, Fructus Gardeniae and Cuminum cyminum in increasing bactericidal capacity. In Comparative Example 4, L-isoleucine was omitted in Example 3, and the bactericidal rate was lower than that of Example 3. It can be seen that L-isoleucine can enhance the bactericidal capacity of Taraxacum mongolicum, Fructus Gardeniae and Cuminum cyminum. In Comparative Example 5, glutamine was omitted in Example 3, and the bactericidal rate was lower than that of Example 3, but the difference was not very significant. It can be seen that although glutamine can enhance the bactericidal capacity of Taraxacum mongolicum, Fructus Gardeniae and Cuminum cyminum, the effect is general.

[0109] (2) Gastric mucosa repair capacity test

[0110] 110 clean male Kunming mice (half male and half female) were randomly divided into 11 groups: blank group, model group, experimental group 1, experimental group 2, experimental group 3, control group 1, control group 2, control group 3, control group 4, control group 5, control group 7, 10 in each group. Refer to the article (Huang Y, Hang X, Jiang X, Zeng L, Jia J, Xie Y, Li F, Bi H. In Vitro and In Vivo Activities of Zinc Linolenate, a Selective Antibacterial Agent against Helicobacter pylori. Antimicrob Agents Chemother [J]. 2019; 63 (6): e00004-19) to construct the in vivo model of Helicobacter pylori NSH57 strain infected mice, after the modeling is successful, the mice in each group are treated with the treatment drugs by gavage, and the blank group and the model group are treated with distilled water by gavage. After 14 days, all mice were fasted for 24 hours, then executed by cervical dislocation, then dissected the mice, cut open the stomach tissue along the greater curvature, cut 2 pieces of antral tissue and 2 pieces of body tissue, and observed the gastric mucosa pathology respectively.

[0111] Results suggest: the blank group of mice gastric mucosa epithelium is complete, a few of lymphocytes can be seen in the mucosa bottom of part of mice antrum, the inflammation degree of model group of mice antrum is heavier, more lymphocytes, eosinophilic granulocyte and neutrophil infiltration can be seen, the inflammation degree of each treatment group of mice antrum is improved compared with model group (P<0.05), among them, the gastric mucosa tissue of experimental group 1, experimental group 2, experimental group 3 is improved most obviously, and the inflammation almost disappears (P<0.01), the inflammation degree of control group 3 is the heaviest among control groups, a few of lymphocytes, eosinophilic granulocyte and neutrophil infiltration can be seen, the inflammation degree of control group 2 and control group 1 is lighter compared with control group 3, a few of lymphocytes, eosinophilic granulocyte and neutrophil infiltration can be seen, and the inflammation degree of control group 2 is relatively light; the inflammation degree of control group 5 is a little lighter than control group 2, and the inflammation degree of control group 4 and control group 7 is the lightest among control groups, a few of lymphocytes and eosinophilic granulocyte and neutrophil infiltration can be seen, in summary, Hericium erinaceus can inhibit the growth of Helicobacter pylori and reduce its damage to gastric mucosa, Grifola frondosa can enhance the immunity of the body, promote the recovery and repair of the inflammation site, Euryale ferox has various pharmacological effects such as antioxidant, anti-fatigue, improving immunity, etc., it can promote the peristalsis of the intestinal tract and improve the gastric cells at the same time, soybean oligopeptide can reduce the expression of inflammatory factors and improve the immunity of the body, L-isoleucine can affect the activity and function of immune cells, promote the production of antibodies and cellular immune response, glutamine has the protection and repair effect on gastric and intestinal mucosa damage, and is beneficial to the recovery of intestinal absorption function and immune function of the body, the five have a mutual potentiation relationship, which can quickly repair the gastric mucosa damage, and the gastric mucosa repair ability of Euryale ferox powder enzymatic powder is better than that of Euryale ferox powder itself.

[0112] (3) Taste test

[0113] The products obtained in Example 1-Example 3, Comparative Example 8 were subjected to sensory evaluation test, the evaluation group was composed of 20 evaluators meeting the screening standards and conditions of the evaluators in sensory analysis, scoring from sweetness (20 points), taste (30 points), smell (30 points), appearance (20), using percentage scoring standard, see Table 2, the sensory score was the average value.

[0114] Table 2 Score standard table of calcium supplement nutritional product

[0115]

[0116] Results:

[0117] Example 1: appearance is smooth, particle size is uniform, taste is delicate, entry is smooth, easy to swallow, no odor, sweet taste is moderate, no aftertaste, comprehensive score is 96.

[0118] Example 2: appearance is neat, particle size is uniform, mouthfeel is delicate, entry is smooth, easy to swallow, no peculiar smell, sweet taste is moderate, no after bitterness, comprehensive score is 95.

[0119] Example 3: appearance is neat, particle size is uniform, mouthfeel is delicate, entry is smooth, easy to swallow, no peculiar smell, sweet taste is moderate, no after bitterness, comprehensive score is 96.

[0120] Comparative Example 8: appearance is rough, particle size is not uniform, mouthfeel is rough, has dry powder, sticky mouth, no peculiar smell, has bitterness, comprehensive score is 47.

[0121] It can be seen that the preparation process of the present application can well ensure the uniformity and delicacy of the obtained microparticles and the mouthfeel.

[0122] The specific embodiments of the present application are described above. It should be understood that the present application is not limited to the specific embodiments described above, and various modifications or changes can be made by those skilled in the art within the scope of the claims, which does not affect the essential content of the present application.

Claims

1. A Hericium erinaceus composition for use against Helicobacter pylori, characterized in that: Prepared from the following components by weight parts: Hedgehog mushroom 20-35 parts, dandelion 10-20 parts, Grifola 20-30 parts, okra tender pod 20-30 parts, mountain gardenia 10-15 parts, Euryale 10-15 parts, cumin 5-10 parts, and a blending agent 25.6-51.3 parts; wherein the blending agent includes: soybean oligopeptide 5-10 parts, L-isoleucine 0.1-0.3 parts, glutamine 0.5-1 part, erythritol 20-40 parts; in preparation, the following steps are included: S1, take the formula amount of hedgehog mushroom, Grifola, Euryale, grind the powder respectively, and mix and stir the hedgehog mushroom powder and Grifola powder uniformly to obtain the hedgehog mushroom-Grifola powder and Euryale powder; S2, take the formula amount of dandelion, mountain gardenia, and cumin, crush and treat, then place in an ultrasonic extractor, add appropriate amount of purified water according to the solid-liquid ratio of 1:20, ultrasonic extraction under the conditions of 60-70℃ and power 350-400w for 30-40 min, filter, reserve the filtrate, take the residue, place in an enzymatic hydrolysis tank, add appropriate amount of purified water according to the solid-liquid ratio of 1:10, add appropriate amount of plant compound hydrolytic enzyme according to the proportion of 5%-6%, enzymatic hydrolysis at 40-50℃ for 2-3h, filter, combine the two filtrates to obtain the extraction liquid; S3, take the formula amount of okra tender pod, cut into pieces, place in a high-pressure tank, use saturated water vapor as medium, maintain pressure at 0.6-1.0Mpa for 120-150s, quickly restore to normal pressure, dry to obtain okra tender pod powder; S4, take the okra tender pod powder, extract by acid hydrothermal extraction method, centrifugal separation to obtain okra tender pod residue and supernatant, add ethanol to the supernatant to precipitate, centrifugal separation to obtain the precipitate to obtain okra pectin; S5, take the okra tender pod residue, place in an enzymatic hydrolysis tank together with the Euryale powder, add appropriate amount of purified water and neutral protease, hydrolyze at pH8.0, temperature 45℃, substrate concentration 5%, enzyme amount 4000 U / g for 3h, then hydrolyze with alkaline protease at pH8.5, temperature 50℃, substrate concentration 3%, enzyme amount 5000 U / g for 3h, spray dry to obtain the enzymatic hydrolysis powder; S6, mix and stir the obtained hedgehog mushroom-Grifola powder, extraction liquid, and enzymatic hydrolysis powder uniformly, concentrate, granulate, dry, and sieve once to obtain pre-made microparticles; S7, dissolve the formula amount of soybean oligopeptide, L-isoleucine, glutamine, and erythritol in 3 times amount of water, mix uniformly with the obtained okra pectin by ultrasonic to obtain a coating liquid; S8, preheat the pre-made microparticles at 40℃ for 3 min, place in a fluidized bed coating machine, use bottom spraying type, spraying speed is 4.0-5.0mL / min, air inlet frequency is 30-40 Hz, the temperature in the fluidized bed coating machine is maintained at 30-40℃ all the time, after coating, continue to dry the coated microparticles in the fluidized bed for a period of time and take out, and the product is obtained.

2. The Hericium himalaicum composition of claim 1, wherein: Prepared from the following components by weight parts: Hericium erinaceus 20 parts, dandelion 10 parts, Grifola frondosa 20 parts, okra tender pod 20 parts, mountain gardenia 10 parts, Euryale 10 parts, cumin 5 parts, soybean oligopeptide 5 parts, L-isoleucine 0.1 part, glutamine 0.5 part, erythritol 20 parts.

3. The Hericium mushroom composition of claim 1, wherein the composition is effective against H. pylori. The obtained product is prepared by the following components by weight fraction: Hericium erinaceus 35 parts, dandelion 20 parts, Grifola frondosa 30 parts, okra tender pod 30 parts, mountain gardenia 15 parts, Euryale 15 parts, cumin 10 parts, soybean oligopeptide 10 parts, L-isoleucine 0.3 part, glutamine 1 part, erythritol 40 parts.

4. The Hericium mushroom composition of claim 1, wherein the composition is effective against H. pylori. 5 The obtained product is prepared by the following components by weight fraction: Hericium erinaceus 17.5 parts, dandelion 15 parts, Grifola frondosa 25 parts, okra tender pod 25 parts, mountain gardenia 12.5 parts, Euryale 12.5 parts, cumin 7.5 parts, soybean oligopeptide 7.5 parts, L-isoleucine 0.2 part, glutamine 0.75 part, erythritol 30 parts.

5. The Hericium erinaceus composition for combating Helicobacter pylori as described in claim 1, characterized in that: In the step S4, the okra tender pod powder is mixed with hydrochloric acid with pH of 2-5 at the ratio of 1g:10-20mL of solid-liquid ratio, and stirred and extracted at 50-80℃ for 50-120min; the centrifugal separation rate is 5000-7000r / min, and the centrifugal time is 20-40min; the solid is extracted by acid hydrothermal extraction, then centrifuged and separated, and the two supernatants are combined.

6. The Hericium erinaceus composition for combating Helicobacter pylori as described in claim 1, characterized in that: In the step S4, the ethanol is 75-95v% ethanol in volume concentration, and the volume ratio of ethanol to supernatant is 1-3:1; the centrifugal separation rate is 5000-7000r / min, and the centrifugal time is 20-40min; the precipitate after centrifugal separation is washed 2-3 times with 40-50v% ethanol in volume concentration.

7. The Hericium mushroom composition of claim 1, wherein the composition is effective against H. pylori. 5 In the step S8, the coated pellets are dried to the water content of 1%. In the step S4, the okra tender pod powder is mixed with hydrochloric acid with pH of 2-5 at the ratio of 1g:10-20mL of solid-liquid ratio, and stirred and extracted at 50-80℃ for 50-120min; the centrifugal separation rate is 5000-7000r / min, and the centrifugal time is 20-40min; the solid is extracted by acid hydrothermal extraction, then centrifuged and separated, and the two supernatants are combined.

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