A composition having an anti-melanogenic activity and a method for preparing the same
A solid dispersion of psoralen coumarin was prepared by combining freeze centrifugation with Euryale ferox extract, which solved the problems of low extraction efficiency and poor solubility in existing technologies and achieved a more efficient anti-melanin production inhibition effect.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- CHANGZHOU UNIV
- Filing Date
- 2023-11-23
- Publication Date
- 2026-05-19
AI Technical Summary
Existing methods for extracting psoralen and coumarin suffer from problems such as high equipment costs, solvent residue, low extraction efficiency, and high volatility. Furthermore, when used alone, they are not effective in inhibiting melanin production.
Psoralen coumarin was extracted using freeze centrifugation, and a solid dispersion of the psoralen coumarin was prepared using Euryale ferox extract as the dispersion medium. Combined with the purification process of Euryale ferox extract, the extraction efficiency and solubility were improved.
It improved the extraction efficiency and solubility of psoralen coumarin components, enhanced the inhibitory effect on melanin production, solved the problems of inconvenient storage and use, and achieved better anti-melanin production activity.
Smart Images
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Abstract
Description
Technical Field
[0001] This invention relates to the field of traditional Chinese medicine, specifically to a composition with anti-melanin production activity and its preparation method. Background Technology
[0002] Psoralea corylifolia is the dried, mature seed of the plant. Its main medicinal component is volatile oil, which is primarily composed of coumarin compounds such as psoralen and psoralen. In recent years, the cosmetic and skincare effects of Psoralea corylifolia have received widespread attention.
[0003] Tyrosinase is a key rate-limiting enzyme in melanin synthesis, and most skin-whitening cosmetics are developed using tyrosinase inhibitors as whitening agents. Studies have shown that the coumarin component of psoralea corylifolia has inhibitory effects on both tyrosine monophenolase and diphenolase, and can treat hyperpigmentation after skin inflammation. Furthermore, some coumarin components in psoralea corylifolia have a potential inhibitory effect on melanin formation in B16 mouse melanoma cells induced by α-melanocyte-stimulating hormone (MSH). Therefore, the coumarin components of psoralea corylifolia may be a potential effective skin whitening agent. Thus, the coumarin components of psoralea corylifolia have significant application value in the cosmetic industry.
[0004] The coumarins in Psoralea corylifolia are mostly volatile oils. Commonly used extraction methods for volatile oils include supercritical CO2 extraction, solvent extraction, and pressing. Supercritical fluid extraction generally uses CO2 as the extraction solvent. Although this method yields complete components, the equipment is expensive, resulting in high costs. Solvent extraction has disadvantages such as solvent residue and difficult recovery. Pressing yields relatively complex components, and the purity of the volatile oil is relatively low. In addition, volatile oils are highly susceptible to volatilization, oxidation, and deterioration during later storage. Summary of the Invention
[0005] To overcome the shortcomings of existing technologies, this invention provides a composition with anti-melanin production activity, using Euryale ferox extract as a dispersion medium to prepare a solid dispersion of psoralen coumarin components. To achieve the above objective, the technical solution adopted by this invention is as follows:
[0006] The extraction method of psoralen coumarin components includes the following steps:
[0007] (1) Pretreatment: Take naturally dried Psoralea corylifolia medicinal material, crush it, pass it through a 40-mesh sieve, add 8 to 12 times the amount (V / W) of distilled water, and sonicate it at 20℃ for 10 minutes. Then steam distill for 2 to 4 hours, collect the upper oily liquid in the volatile oil extractor, and weigh the obtained Psoralea corylifolia volatile oil.
[0008] (2) Alkali dissolution: Add a 2% to 4% NaOH solution to the volatile oil obtained in step (1), with a volume-to-mass ratio of 15:1 to 30:1 (V / W) to the volatile oil of Psoralea corylifolia, and shake to dissolve it completely.
[0009] (3) Acid precipitation: Add 5% HCl to the solution obtained in step (2), adjust the pH to 5-6, and let it stand for 12 hours.
[0010] (4) Refrigerated centrifugation: Transfer the solution obtained in step (3) into a centrifuge and centrifuge at a temperature of 2-4°C, a speed of 2000-4000 rpm, and a centrifugation time of 6-8 min. Collect the precipitate and wash it with distilled water until neutral. The psoralen-coumarin component is obtained.
[0011] The extraction method of Euryale ferox extract includes the following steps:
[0012] (1) Take the seeds of Euryale ferox and dry them naturally. Crush them and add 1:6 to 1:10 (W / V) of distilled water to extract them by reflux for 1 hour. Filter the solution and let the filtrate cool.
[0013] (2) Slowly add ethanol to the extract in step (1) until the final concentration of ethanol in the extract reaches 70% to 80%. Let it stand for 12 hours, centrifuge, and take the precipitate.
[0014] (3) Dissolve the precipitate from step (3) in an appropriate amount of distilled water to prepare a solution with a mass concentration of 30 mg / mL, and then purify it using HP20 macroporous resin. The sample-to-macroporous resin loading ratio is V. 样品 / W 大孔树脂 =4:1, the sample solution was loaded twice repeatedly. After loading, the sample was allowed to stand for 0.5 hours, and then eluted with distilled water. First, eluted with distilled water for 1 column volume and discarded; then eluted with distilled water for 4 column volumes, collected the eluent, recovered the solvent, and freeze-dried in a freeze dryer to obtain freeze-dried powder of Euryale ferox extract.
[0015] Preparation of solid dispersions of psoralen coumarin components:
[0016] (1) Weigh the freeze-dried powder of Euryale ferox extract according to the mass ratio, add a small amount of distilled water, stir to dissolve it, and obtain the solid dispersion medium part;
[0017] (2) Take the psoralen coumarin component according to the mass ratio, dissolve it in a small amount of ethanol, and slowly add it dropwise to the dispersion medium in step (1). After it dissolves, place it in a rotary evaporator to recover the solvent under reduced pressure, and dry it to obtain a uniform solid dispersion of psoralen coumarin component, which is the composition of the present invention.
[0018] Beneficial effects
[0019] (1) The extraction method of psoralen coumarin component of the present invention adopts freeze centrifugation technology, which reduces the loss of volatile oil and thus improves the extraction efficiency.
[0020] (2) This invention uses Euryale ferox extract as a dispersion medium to prepare a solid dispersion of psoralen coumarin components. This not only makes good use of the bioactivity of the two medicinal materials, enabling them to synergistically enhance each other, but also improves the solubility of the psoralen coumarin components (dissolution test, see...). Figure 2 This solved the practical problems of its poor preservation and inconvenience in use. Attached image description:
[0021] Figure 1 For Example 1 and Comparative Examples 1-4, the melanin content of different dosage groups in the MTT experiment.
[0022] Figure 2 The in vitro dissolution curves are for Example 1 and Comparative Example 1 and Comparative Example 3.
[0023] Figure 3 The following are thin-layer chromatograms of Examples 1, 3, 1, and 2. Detailed Implementation
[0024] The present invention will be further described below with reference to embodiments, but is not limited thereto.
[0025] Example 1
[0026] (1) Take naturally dried Psoralea corylifolia, crush it, pass it through a 40-mesh sieve, add 12 times the amount (V / W) of distilled water, pre-treat it with ultrasound at 20℃ for 10 minutes, then extract it by steam distillation for 4 hours, collect the upper oily liquid in the volatile oil extractor, and weigh the obtained Psoralea corylifolia volatile oil.
[0027] (2) Add 30 times (V / W) of 2% NaOH solution to the volatile oil obtained above, shake, and let it dissolve completely.
[0028] (3) Add 5% HCl to the solution obtained in step (2) to adjust the pH to 6 and let it stand for 12 hours.
[0029] (4) Transfer the solution obtained in step (3) to a centrifuge and centrifuge at 2°C, 4000 rpm for 8 min. Collect the precipitate and wash it with distilled water until neutral. The psoralen-coumarin component is obtained.
[0030] (5) Take the seeds of Euryale ferox and dry them naturally, crush them, pass them through a 20-mesh sieve, add 10 times the amount (W / V) of distilled water and reflux for 1 hour, filter them, and let the filtrate cool.
[0031] (6) Slowly add ethanol to the above extract to make the final concentration of ethanol in the extract reach 75%, let stand for 12 hours, centrifuge, and separate the precipitate.
[0032] (7) Dissolve the above precipitate in an appropriate amount of distilled water to prepare a solution with a mass concentration of 30 mg / mL, and purify it using HP20 macroporous resin. The sample-to-macroporous resin loading ratio is V. 样品 / W 大孔树脂 =4:1, the sample solution was loaded twice repeatedly. After loading, the sample was allowed to stand for 0.5 hours, and then eluted with distilled water. First, eluted with distilled water for 1 column volume and discarded; then eluted with distilled water for 4 column volumes, collected the eluent, recovered the solvent, and freeze-dried in a freeze dryer to obtain freeze-dried powder of Euryale ferox extract.
[0033] (8) Weigh the freeze-dried powder of Euryale ferox extract according to a mass ratio of 1:4 (psoralen coumarin component: Euryale ferox extract), add distilled water, stir to dissolve, and obtain the solid dispersion medium part; separately take the psoralen coumarin component, dissolve it in ethanol, slowly add it dropwise to the above dispersion medium, and after it dissolves, place it in a rotary evaporator to recover the solvent under reduced pressure, dry it, and obtain a uniform solid dispersion of psoralen coumarin component, which is the traditional Chinese medicine composition of the present invention.
[0034] Anti-melanin production experiment
[0035] Anti-melanogenesis activity was investigated using the MTT assay. Logarithmic growth phase B16 melanoma cells were digested and added to six-well plates at a seeding concentration of 5 × 10⁶ cells / well. 5 Cells / mL, incubated at constant temperature for 24 hours.
[0036] Accurately weigh the samples to be tested, add PBS, sonicate to dissolve, and filter to prepare test solutions of a certain concentration. Add 2 mL to each well of a six-well plate according to the dilution method to make the final concentrations of the test samples 50 μg / mL and 100 μg / mL, respectively. Use PBS as a blank control and continue to incubate at 37℃ and 5% CO2 for 48 h.
[0037] Wash twice with PBS, lyse at 4°C for 20 min, scrape off the bottom layer of cells, centrifuge for 10 min, discard the supernatant, and add 100 μL of 1% NaOH lysis buffer to the total melanin in the cells. Lyse for 1-2 h, and vortex to mix after lysis. Set up three replicates for each group, and measure the absorbance at 490 nm using a microplate reader. Calculate the inhibition rate of melanin production by the test sample according to the following formula. The melanin content and inhibition rate of melanin production in the cells of Example 1 and Comparative Examples 1-4 are shown in Table 1.
[0038]
[0039] Comparative Example 1
[0040] The preparation method is the same as in Example 1, except that the psoralen-coumarin component is not subjected to freeze centrifugation; the precipitate after the alkaline extraction and acid precipitation steps is directly separated as the psoralen-coumarin component. The anti-melanin production experiment is the same as in Example 1.
[0041] Comparative Example 2
[0042] The preparation method is the same as in Example 1, except that the Psoralea corylifolia herb was not pretreated with ultrasound before steam distillation extraction. The anti-melanin production experiment is the same as in Example 1.
[0043] Comparative Example 3
[0044] The preparation method is the same as that for the psoralen coumarin component in Example 1, except that this extract contains only the effective fraction of psoralen. The anti-melanin production experiment is the same as in Example 1.
[0045] Comparative Example 4
[0046] The preparation method is the same as that for the preparation of the Euryale ferox extract in Example 1, except that this extract contains only Euryale ferox extract. The anti-melanin production experiment is the same as in Example 1.
[0047] Table 1. Melanin content and inhibition rate of melanin production in samples from Example 1 and Comparative Examples 1-4.
[0048]
[0049] As shown in Table 1, the high-dose group (100 μg / mL) in Example 1 significantly inhibited melanin production, with an inhibition rate of 65.56%, which was highly significant compared to the blank group (**P < 0.01). In contrast, the low-dose groups in Comparative Examples 1-2 showed no significant inhibition, while the high-dose groups had inhibition rates of 52.00% and 55.31%, respectively, both lower than those in Example 1. This indicates that the ultrasonic pretreatment of the medicinal material before steam distillation is essential in the extraction process of psoralen coumarin components. Furthermore, the freeze-centrifugation step of the obtained volatile oil is also crucial, directly affecting the efficacy of the composition.
[0050] Comparative studies 3-4 showed that both the low-dose and high-dose groups exhibited relatively low inhibition rates on melanin production, with no significant inhibitory effect. This indicates that the combination of the two extracts did indeed enhance the inhibitory effect on melanin production, demonstrating a synergistic effect.
[0051] Determination of psoralen content
[0052] The determination was performed according to the High Performance Liquid Chromatography (HPLC) method (General Rule 0512) of the Pharmacopoeia of the People's Republic of China (2020 Edition, Part I). Chromatographic conditions: C18 column (4.6 mm × 180 mm, 4 μm); mobile phase: acetonitrile:water (66:34); flow rate: 0.6 mL / min. -1 Column temperature: 25℃; Detection wavelength: 254nm. Accurately weigh an appropriate amount of psoralen reference standard and dissolve it in methanol to prepare a solution containing 20 μg per mL. Separately, accurately weigh approximately 0.5 g of the test sample powder, add an appropriate amount of methanol, and extract by sonication (250W, frequency: 55kHz, 25℃) for 1 hour. Transfer the extract to a 100 mL volumetric flask, add methanol to the mark, shake well, filter, and collect the filtrate to obtain the test solution. Accurately inject 10 μL each of the reference solution and the test solution into the high-performance liquid chromatograph (HPLC) and determine the psoralen content in each sample using the external standard method.
[0053] Table 2 shows the psoralen content in the samples of Examples 1, 3, and Comparative Examples 1-2.
[0054] Table 2
[0055] Test sample psoralen content Example 1 141.551 mg / g Example 3 139.226 mg / g Comparative Example 1 106.437 mg / g Comparative Example 2 127.038 mg / g
[0056] As shown in Table 2, the psoralen content of each sample in Examples 1, 3 and Comparative Examples 1-2 is different. The psoralen content in Example 1 is the highest, indicating that in the extraction process of psoralen coumarin components, the freezing centrifugation and ultrasonic pretreatment operations are helpful for the extraction and separation of effective components.
[0057] In vitro dissolution test
[0058] The dissolution test was performed according to Method II of the Pharmacopoeia of the People's Republic of China (2020 Edition, Part IV). The dissolution apparatus rotation speed was set to 100 r / min. -1 The water bath temperature of the dissolution apparatus was adjusted to a constant 37±0.2℃. 0.3% sodium dodecyl sulfate was used as the dissolution medium, with a volume of 900 mL per group. The test sample was added to the dissolution apparatus, and 4 mL samples were taken at 10 min, 20 min, 40 min, 60 min, 80 min, 100 min, 120 min, and 180 min. Immediately after sampling, 4 mL of 0.3% sodium dodecyl sulfate was added. The samples were centrifuged at 12000 r·min. -1 Centrifuge for 5 minutes. Aspirate the supernatant and filter through a microporous membrane for later use. Determine the psoralen content in the sample according to the method for psoralen content determination, convert it to cumulative dissolution rate, and plot the in vitro dissolution curve. The in vitro dissolution curves of psoralen in samples from Example 1, Comparative Example 1, and Comparative Example 3 are shown below. Figure 2 .
[0059] Depend on Figure 2As can be seen, the in vitro dissolution rate of psoralen in Samples 1 and 1 (Comparative Example 1) is relatively good, with a cumulative dissolution rate of over 90% after about 100 minutes. In contrast, the in vitro dissolution rate of psoralen in Sample 3 is poor, with a cumulative dissolution rate of only about 30% after 180 minutes. This indicates that the in vitro dissolution rate of psoralen-coumarin components is not ideal when used alone. However, by combining psoralen-coumarin components with Euryale ferox extract to prepare a solid dispersion, the in vitro dissolution rate is greatly improved, thus enhancing solubility.
[0060] Thin-layer chromatography detection of psoralen coumarin components
[0061] (1) Preparation of reference solution
[0062] Accurately weigh 10 mg of psoralen reference standard and dilute to 1 mL with methanol to prepare a reference standard solution with a concentration of 0.0100 g / mL.
[0063] (2) Preparation of the test solution
[0064] Weigh 20 mg of each of the samples from Example 1 (labeled "3" on the thin-layer plate), Example 3 (labeled "6" on the thin-layer plate), Comparative Example 1 (labeled "sample" on the thin-layer plate), and Comparative Example 2 (labeled "9" on the thin-layer plate), add 1 mL of methanol, shake to dissolve, and filter to obtain the final product.
[0065] (3) Preparation of developing solvent
[0066] Measure 10 mL of petroleum ether and ethyl acetate in a 5:1 ratio and saturate them in a chromatography tank for 10 min.
[0067] (4) Sampling, unfolding, and identification
[0068] Apply an appropriate amount of each sample to a capillary tube and spot it. After development, observe the fluorescent spots under ultraviolet light (254nm).
[0069] From the appendix Figure 2 It can be seen that the samples tested, including Example 1, Example 3 and Comparative Examples 1-2, all contained psoralen, the main component of psoralen, but the psoralen content varied among the samples.
[0070] Example 2
[0071] The preparation method of this embodiment is the same as that of Example 1. The difference is that the mass ratio of the two extracts (lyophilized powder and euryale seed extract) is different when preparing the solid dispersion composition. The specific combination ratio, melanin content and inhibition rate of melanin production are shown in Table 3. The anti-melanin production experiment is the same as that of Example 1.
[0072] Table 3. Melanin content and inhibition rate of melanin production in each group of samples from Examples 1-2.
[0073]
[0074] As shown in Table 3, when the ratio of psoralen coumarin to Euryale ferox extract (W:W) is less than 1:3, the inhibition rate of melanocyte production is not significant (below 50%) in either the high-dose or low-dose group. When the ratio is 1:3, 1:4, or 1:5, the inhibition rate of the high-dose group is improved, especially when the ratio is 1:4, the inhibition rate of melanocyte production is more significant. Therefore, the final mass ratio of psoralen coumarin to Euryale ferox extract in this invention is set at 1:4.
[0075] Example 3
[0076] (1) Take naturally dried Psoralea corylifolia, crush it, pass it through a 40-mesh sieve, add 10 times the amount (V / W) of distilled water, pre-treat it with ultrasound at 20℃ for 10 minutes, then extract it by steam distillation for 3 hours, collect the upper oily liquid in the volatile oil extractor, and weigh the obtained Psoralea corylifolia volatile oil.
[0077] (2) Add 30 times (V / W) of 2% NaOH solution to the volatile oil obtained above, shake, and let it dissolve completely.
[0078] (3) Add 5% HCl to the solution obtained in step 2, adjust the pH to 6, and let it stand for 12 hours.
[0079] (4) Transfer the solution obtained in step 3 to a centrifuge and centrifuge at 2°C, 4000 rpm for 8 min. Collect the precipitate and wash it with distilled water until neutral. The psoralen-coumarin fraction is obtained.
[0080] (5) Take the seeds of Euryale ferox and dry them naturally, crush them, pass them through a 20-mesh sieve, add 10 times the amount (W / V) of distilled water and reflux for 1 hour, filter them, and let the filtrate cool.
[0081] (6) Slowly add ethanol to the above extract to make the final concentration of ethanol in the extract reach 75%, let stand for 12 hours, centrifuge, and separate the precipitate.
[0082] (7) Dissolve the above precipitate in an appropriate amount of distilled water to prepare a solution with a mass concentration of 30 mg / mL, and purify it using HP20 macroporous resin. The sample-to-macroporous resin loading ratio is V. 样品 / W 大孔树脂 =4:1, the sample solution was loaded twice repeatedly. After loading, the sample was allowed to stand for 0.5 hours, and then eluted with distilled water. First, eluted with distilled water for 1 column volume and discarded; then eluted with distilled water for 4 column volumes, collected the eluent, recovered the solvent, and freeze-dried in a freeze dryer to obtain freeze-dried powder of Euryale ferox extract.
[0083] (8) Weigh the freeze-dried powder of Euryale ferox extract at a mass ratio of 1:4, add a small amount of distilled water, stir to dissolve, and obtain the solid dispersion medium part; separately take the psoralen coumarin component, add a small amount of ethanol to dissolve, slowly add it dropwise to the above dispersion medium, and after it dissolves, place it in a rotary evaporator to recover the solvent under reduced pressure, dry, and obtain a uniform solid dispersion of psoralen coumarin component, which is the traditional Chinese medicine composition of the present invention.
[0084] The melanin content and inhibition rate of melanin production in the sample of Example 3 are shown in Table 3. The anti-melanin production experiment is the same as that in Example 1.
[0085] Example 4
[0086] The preparation method of this embodiment is the same as that of Example 3. The difference is that the amount of alkali and the pH of acidification are different when preparing the psoralen coumarin component. The melanin content and inhibition rate of melanin production of the compositions prepared under different conditions are shown in Table 4. The anti-melanin production experiment is the same as that of Example 1.
[0087] Table 4. Melanin content and inhibition rate of melanin production in each group of samples from Examples 3-4.
[0088]
[0089] As shown in Table 4, during the alkaline extraction and acid precipitation treatment of Psoralea corylifolia volatile oil, adding 30 times (v / w) of 2% NaOH solution to the volatile oil and shaking it until completely dissolved, followed by adjusting the pH to 6 with 5% HCl and allowing it to stand, resulted in the composition of Psoralea coumarin fraction prepared with the best melanin production inhibition effect. Therefore, the optimal process parameters for this step were determined to be adding 30 times the amount of 2% NaOH and acidifying with hydrochloric acid to pH 6.
[0090] Example 5
[0091] The preparation method in this embodiment is the same as that in Example 1, except that the sample loading concentration, loading ratio, and elution method are different when purifying the Euryale ferox extract with macroporous resin. The melanin content and inhibition rate of melanin production of the compositions prepared with different parameters are shown in Table 5. The anti-melanin production experiment is the same as in Example 1.
[0092] Table 5. Melanin content and inhibition rate of melanin production in each group of samples in Example 5.
[0093]
[0094] As shown in Table 5, different parameters resulted in varying inhibition rates of melanin production in the final composition obtained after purification of the Euryale ferox extract using macroporous resin. Considering both the inhibition rate and the economics and convenience of the operation, the optimal purification parameters were determined as follows: Euryale ferox precipitate was dissolved in distilled water to prepare a solution with a mass concentration of 30 mg / mL, and then purified using HP20 macroporous resin. The sample-to-macroporous resin loading ratio was V0. 样品 / W 大孔树脂 =4:1, the sample solution was loaded twice repeatedly. After loading, the sample was allowed to stand for 0.5 hours, and then eluted with distilled water. First, eluted with distilled water for 1 column volume and discarded; then eluted with distilled water for 4 column volumes, collected the eluent, recovered the solvent, and freeze-dried in a freeze dryer to obtain freeze-dried powder of Euryale ferox extract.
Claims
1. A composition having anti-melanin production activity, characterized in that, The composition uses Euryale ferox extract as a dispersion medium and psoralen coumarin component as an active ingredient to prepare a solid dispersion composition of psoralen coumarin component. The mass ratio of psoralen coumarin component to Euryale ferox extract is 1:4; The extraction steps of the psoralen coumarin component are as follows: ① Pretreatment: Take naturally dried Psoralea corylifolia, crush it, sieve it, add distilled water, sonicate it for 10 minutes for pretreatment, and then extract it by steam distillation. Collect the upper oily liquid in the volatile oil extractor to obtain Psoralea corylifolia volatile oil. ② Alkali dissolution: Add a 2%~4% NaOH solution to the volatile oil obtained in step ① and shake until completely dissolved; The volume-to-mass ratio of psoralea corylifolia volatile oil in sodium hydroxide solution is 15:1 to 30:
1. ③ Acid precipitation: Add 5% HCl to the solution obtained in step ②, adjust the pH to 5-6, and let it stand for 12 hours to obtain the solution; ④ Freeze centrifugation: Transfer the solution obtained in step ③ into a centrifuge, freeze centrifuge, collect the precipitate, wash with distilled water until neutral, and obtain the psoralen coumarin component; The extraction steps of foxnut extract are as follows: ① Take the seeds of Euryale ferox and dry them naturally, then crush them. Add distilled water at a mass-to-volume ratio of 1:6 to 1:10, reflux and extract for 1 hour, filter, and let the filtrate cool. ② Add ethanol to the filtrate from step ① to make the ethanol concentration in the extract reach 70%~80%, let stand for 12 hours, centrifuge, and take the precipitate; ③ After dissolving the precipitate from step ② in distilled water, purify it using HP20 macroporous resin. Elute with distilled water for 1 column volume and discard the eluent. Continue eluting with distilled water for 4 column volumes, collect the eluent, recover the solvent, and freeze-dry it in a freeze dryer to obtain freeze-dried powder of Euryale ferox extract. When purifying with HP20 macroporous resin, the sample loading concentration was 30 mg / mL, and the loading ratio was V. 样品 / W 大孔树脂 =4:1, the sample solution was loaded twice, and after loading, it was allowed to stand for 0.5 hours, and then eluted with distilled water.
2. The composition according to claim 1, characterized in that, In step ①, the mass-to-volume ratio of psoralea corylifolia to distilled water is 1:8 to 1:12, the ultrasonic pretreatment temperature is 20℃, and the steam distillation extraction time is 2 to 4 hours.
3. The composition according to claim 1, characterized in that, In step ④, the centrifuge temperature is 2~4℃, the centrifuge speed is 2000~4000 rpm, and the centrifugation time is 6~8 min.
4. A method for preparing the solid dispersion composition as described in claim 1, characterized in that, The preparation method steps are as follows: (1) Weigh the freeze-dried powder of Euryale ferox extract according to the mass ratio, add distilled water, stir to dissolve it, and obtain the solid dispersion medium part; (2) Take the psoralen coumarin component according to the mass ratio, dissolve it in ethanol, and then add it dropwise to the dispersion medium in step (1). After it dissolves, place it in a rotary evaporator to recover the solvent under reduced pressure, and dry it to obtain the product.