Morus alba and phellinus igniarius black tea for enhancing immunity and preparation method and application thereof

Abstract

The application discloses a preparation method of mulberry leaf and phellinus igniarius black tea which is helpful for enhancing immunity, and comprises the following steps: step one, preparing the following raw materials by weight: green tea 100-150 parts, mulberry leaf green tea 45-65 parts, sea buckthorn 40-60 parts, momordica grosvenori 40-60 parts, mulberry phellinus powder 3-8 parts, lotus leaf 5-10 parts, polygonatum 5-8 parts, and gardenia 2-5 parts; step two, mixing with water to obtain mixed tea materials; step three, pile fermentation for 15-20 days, and turning the pile when the pile temperature reaches 50 DEG C; step four, airing; step five, steaming by using steam; step six, forming by using a mold; and step seven, cooling and aging. The preparation method has the technical effect of significantly shortening the black tea production time. The prepared mulberry leaf and phellinus igniarius black tea is helpful for enhancing immunity.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of tea processing. More particularly, the present application relates to a mulberry leaf and phellinus igniarius black tea that helps to enhance immunity, and a preparation method and application thereof. BACKGROUND

[0002] Tea originated in China, and there are six major tea categories, including green tea, black tea, dark tea, yellow tea, white tea, and oolong tea. Tea contains components such as catechin, cholestenone, caffeine, inositol, folic acid, and pantothenic acid, and can improve human health. Tea is known as one of the "three major beverages in the world". Dark tea is one of the six major tea categories in China, and its yield accounts for about one tenth of the total tea yield in China. Mulberry phellinus is a rare medicinal fungus. According to the Records of the Divine Farmer and Compendium of Materia Medica, mulberry phellinus has the medicinal effects of benefiting five internal organs, promoting intestinal and stomach qi, and expelling toxic qi, and is mainly used for treating symptoms such as blood clots, blood clots, prolapse of the anus, blood in the stool, leukorrhea, amenorrhea, and diarrhea due to spleen deficiency. Mulberry phellinus is rich in flavonoids, polysaccharides, terpenes and other active substances, and has the effects of regulating the immune system, delaying aging, resisting tumors, resisting inflammation, protecting the liver, and reducing blood sugar.

[0003] Mulberry leaves are rich in protein, amino acids, and various vitamins and minerals, carbohydrates, and plant fibers, and belong to "medicinal and edible homologous", which are good functional foods, and have the effects of reducing blood pressure, reducing blood lipids, resisting aging, increasing endurance, reducing cholesterol, inhibiting fat accumulation, inhibiting thrombus formation, inhibiting the proliferation of harmful bacteria in the intestine, and inhibiting the generation of harmful oxidants. The most prominent function is to prevent and treat diabetes. At the same time, it also has the functions of relieving cough, removing heat, treating dizziness and blurred vision, eliminating eye fatigue, reducing swelling, clearing blood, treating dysentery, edema, tonifying liver, removing freckles, nourishing skin, and resisting aging.

[0004] Based on the unique effects of mulberry phellinus and mulberry leaves, researchers are committed to research and development in order to develop a mulberry leaf and phellinus igniarius black tea that not only helps to enhance immunity, but also reduces high blood sugar and high blood lipids to meet the needs of different groups of people in the market. Due to the fact that mulberry leaves contain a lot of unique substrates, mulberry tea made only from mulberry leaves has a "green smell" characteristic of its variety. In addition, the soup color of mulberry tea is easy to change into greenish blue or dark blue. Although mulberry tea has many health functions, consumers not only do not feel the aroma of tea leaves, but also are affected by the unpleasant smell, taste and soup color, which affects the consumer's desire to consume, resulting in a lack of competitiveness of mulberry tea in the market.

[0005] The purpose of the present application is to mix mulberry phellinus powder, mulberry green tea, and green tea in a certain proportion, and to process a new type of tea health beverage by using a new processing technology of Guangxi Liupao tea. The product quality not only has the unique quality style of "red, thick, old, and mellow" of dark tea, but also has the superimposed effect of the health functions of mulberry phellinus powder, mulberry green tea, and green tea.

[0006] The raw material for making black tea at present mainly includes: a part of fresh tea leaves is used as raw material, and is first made into green tea for standby; and another part of green tea product is used as raw material. According to the traditional method of making black tea, i.e. green tea → fixation → piling → high-temperature steaming → pressing into shape → drying → standing and aging. The existing research results show that piling is the key process for forming the quality of black tea: through piling, the contents in the raw material tea change in series, such as the transformation of tea polyphenols under the conditions of moisture and heat, the enzymatic oxidation under the action of residual active enzymes, and the differentiation under the action of anaerobic microorganisms in the anaerobic environment. In the above series of reactions, the main internal components in the tea, tea polyphenols, are automatically oxidized to generate the effective components of black tea, theaflavins, thearubigins and theabrownines; i.e. tea polyphenols → theaflavins → thearubigins → theabrownines. The piling time of the existing technology is generally 45-70 days, and the finished black tea needs to be subjected to a relatively long standing and aging time, and at least needs to be subjected to a standing and aging time of more than half a year to form the unique black tea quality style of "red, thick, old and mellow". Therefore, the existing technology has the following defects: 1. The piling time of the existing technology is long, generally needs 45-70 days, and the cost of occupying the site and manual piling is high; 2. The black tea produced by the existing technology needs to be subjected to a standing and aging time of at least half a year to form the unique black tea quality style of "red, thick, old and mellow", and the cost of site and fund is high.

[0007] The present application adopts a new processing technology of Guangxi Liupao tea, which mixes Sanghuang powder, Sangye green tea and green tea according to a certain proportion, and piles and ferments in a constant temperature and humidity environment for 15-20 days to form the unique black tea quality style of "red, thick, old and mellow", and overcomes the problems of long piling time, site occupation and long fund turnover time in the traditional processing of black tea. SUMMARY

[0008] An object of the present application is to solve at least the above problems and to provide at least the advantages to be described later.

[0009] In order to achieve these objects and other advantages according to the present application, a preparation method of Sangye Sanghuang black tea for enhancing immunity is provided, which comprises the following steps:

[0010] Step one, prepare the following raw materials by weight: green tea 100-150 parts, Sangye green tea 45-65 parts, sea buckthorn 40-60 parts, monk fruit 40-60 parts, Sanghuang powder 3-8 parts, lotus leaf 5-10 parts, polygonatum 5-8 parts, and gardenia 2-5 parts;

[0011] Step two, mix the raw materials of step one with water to obtain mixed tea material;

[0012] Step three, pile and ferment the mixed tea material for 15-20 days, and turn over when the piling temperature reaches 50℃;

[0013] Step four, dry;

[0014] Step five, steaming with steam;

[0015] Step six, forming by pressing with a mold;

[0016] Step seven, aging for 40-50 days, and the mulberry leaf and mulberry black tea is obtained.

[0017] Preferably, the water content of the mixed tea materials in step two is 26-30%.

[0018] Preferably, the ambient temperature during the heap fermentation in step three is 40-50℃, and the ambient humidity is 85-95%.

[0019] Preferably, the drying in step four is until the water content is no more than 13%.

[0020] Preferably, the steam temperature during the steaming in step five is no less than 100℃, and the steaming time is 60-120 s.

[0021] The application provides a mulberry leaf and mulberry black tea which is helpful for enhancing immunity, and is prepared based on the above preparation method.

[0022] The application provides an application of the mulberry leaf and mulberry black tea in preparing a product for enhancing immunity.

[0023] The application at least has the following beneficial effects:

[0024] First, the preparation method of the application can significantly shorten the black tea production time.

[0025] Second, the mulberry leaf and mulberry black tea has the unique black tea quality style of "red, thick, aged and mellow" of Liupao tea, and also has the fresh and mellow taste of mulberry leaves, and the "green smell" of mulberry leaves is removed.

[0026] Third, the mulberry leaf and mulberry black tea has the effect of enhancing immunity.

[0027] Other advantages, objects and features of the application will be partly embodied in the following description, and will also be understood by those skilled in the art through research and practice of the application. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 The flow chart of one of the preparation methods of the application;

[0029] Figure 2 The fermentation state schematic diagram of CK, 1-5 groups of the application;

[0030] Figure 3 The tea water state schematic diagram of the black tea of CK, 1-5 groups of the application. DETAILED DESCRIPTION

[0031] The present invention will now be described in further detail with reference to the accompanying drawings, so that those skilled in the art can implement it based on the description.

[0032] It should be noted that, unless otherwise specified, the experimental methods described in the following implementation plan are all conventional methods, and the reagents and materials described are all commercially available unless otherwise specified.

[0033] <Dark Tea Formula>

[0034] The body's immunity refers to its ability to resist external invasions and maintain the stability of its internal environment. It is the physiological response of the body to recognize and eliminate "foreign invaders," and the immune system, composed of immune organs, immune cells, and immune-active components, is the foundation of immunity. Existing research shows that the occurrence of many diseases is closely related to immune system disorders, such as aging, cancer, and depression. Therefore, regulating the body's immunity plays an important role in preventing disease. Based on the research objective of enhancing immunity, this study aims to lay the foundation for the development and market launch of functional dark tea. Existing research has confirmed that liver stagnation syndrome, spleen deficiency syndrome, liver stagnation and spleen deficiency syndrome, and kidney yang deficiency syndrome are related to immunodeficiency in modern medicine, all of which inhibit the body's immunity to varying degrees. This study correlates immunodeficiency models with traditional Chinese medicine syndrome models, mainly including kidney deficiency syndrome, liver stagnation and spleen deficiency syndrome, and blood deficiency syndrome.

[0035] Green tea is made from suitable tea tree shoots. It is unfermented, first subjected to high-temperature fixation, then rolled and dried. It has various health benefits, including clearing heat and relieving summer heat, aiding digestion and resolving phlegm, detoxifying and sobering up, promoting saliva production and quenching thirst, reducing internal heat and improving eyesight, and stopping diarrhea and removing dampness.

[0036] Mulberry leaf green tea is made primarily from the leaves of the mulberry tree (Moraceae family), through a series of processing steps including rolling and fixation. It is believed to have benefits such as lowering blood sugar, dispelling wind and clearing heat, reducing inflammation and killing bacteria, and clearing the liver and improving eyesight.

[0037] Sea buckthorn is a traditional Chinese medicine used by the Mongolian and Tibetan people. It is the dried, mature fruit of the sea buckthorn plant (Hippophae rhamnoides). The fruit is harvested in autumn and winter when it is ripe or frozen solid. Impurities are removed, and the fruit is dried or steamed and then dried. It is sour, astringent, and warm in nature. It enters the spleen, stomach, lung, and heart meridians. It strengthens the spleen and aids digestion, relieves cough and phlegm, and promotes blood circulation and removes blood stasis. It is used for spleen deficiency with poor appetite, abdominal pain due to food stagnation, cough with excessive phlegm, chest pain, amenorrhea due to blood stasis, and traumatic injuries with bruises.

[0038] Monk fruit (Luo Han Guo) is the dried fruit of the *Siraitia grosvenorii* plant, belonging to the Cucurbitaceae family. It is harvested in autumn when the fruit turns from light green to dark green, and after drying for several days, it is dried at a low temperature. It is sweet and cool in nature. It enters the lung and large intestine meridians. It clears heat and moistens the lungs, benefits the throat and voice, and lubricates the intestines to relieve constipation. It is used for dry cough due to lung heat, sore throat and loss of voice, and constipation due to intestinal dryness.

[0039] Sanghuang powder is the powder of the fruiting body of Sanghuang, which belongs to the kingdom Fungi, Basidiomycota, and Phanerochaete. Its main functions include immune regulation, anti-cancer, anti-fibrosis, antioxidant, blood sugar reduction, and liver protection.

[0040] Lotus leaf is the dried leaf of Nelumbo nucifera Gaertn. It is collected in summer and autumn, dried to 70-80%, removed from petiole, folded into half circle or fan shape, and dried. It is bitter and cold, and belongs to the liver, spleen, and stomach meridians. It can clear heat and dampness, raise clear yang, and cool blood to stop bleeding. It is used for heat and thirst, dampness diarrhea, spleen deficiency diarrhea, blood heat and hemoptysis, and metrorrhagia. Lotus leaf charcoal can astringe and stop bleeding. It is used for hemorrhagic diseases and postpartum blood dizziness.

[0041] Yuzhu is the dried rhizome of Polygonatum sibiricum. It is harvested in autumn, removed from the root, washed, dried until soft, repeatedly kneaded and dried until there is no hard core, or steamed until transparent, and dried. It is sweet and cold, and belongs to the lung and stomach meridians. It can nourish yin, moisten dryness, and relieve thirst. It is used for lung and stomach yin deficiency, dry heat cough, dry throat and mouth, and internal heat and thirst.

[0042] Zi Zi is the dried mature fruit of Gardenia jasminoides. It is harvested in September-November when the fruit is mature and red-yellow, removed from the peduncle and impurities, steamed until the gas is released or slightly boiled in boiling water, removed, and dried. It is bitter and cold, and belongs to the heart, lung, and Sanjiao meridians. It can clear heat, remove heat, clear heat and dampness, cool blood and detoxify; externally used for swelling and pain. It is used for heat and thirst, dampness diarrhea, spleen deficiency diarrhea, blood heat and hemoptysis, and metrorrhagia. It is used for swelling and pain.

[0043] With kidney deficiency, liver stagnation and spleen deficiency, and blood deficiency as the treatment target, 8 kinds of raw materials such as green tea, mulberry leaf green tea, and Sanghuang powder are matched based on the principle of monarch, minister, and assistant. Green tea, mulberry leaf green tea, sea buckthorn, and monk fruit are used as the monarch, and the function is to regulate the spleen and blood; Sanghuang powder, lotus leaf, yuzhu, and gardenia are used as the minister, and the function is to benefit qi, move qi, and activate blood. The mulberry leaf and Sanghuang black tea formula is obtained: green tea 100-150 parts, mulberry leaf green tea 45-65 parts, sea buckthorn 40-60 parts, monk fruit 40-60 parts, Sanghuang powder 3-8 parts, lotus leaf 5-10 parts, yuzhu 5-8 parts, and gardenia 2-5 parts.

[0044] <Black tea preparation>

[0045] 1. The five groups of mulberry leaf and Sanghuang black tea formula and the control group are shown in Table 1:

[0046] Table 1 Mulberry leaf and Sanghuang black tea formula

[0047]

[0048] 2. The control parameters in the preparation and processing process of the five groups of mulberry leaf and Sanghuang black tea formula are shown in Table 2:

[0049] Table 2 Processing control parameters of mulberry leaf and Sanghuang black tea formula

[0050]

[0051] 3、As Figure 1 shown, the preparation method of mulberry leaf and phellinus igniarius black tea, comprising the following steps:

[0052] Step one, according to table 1, respectively weighing the preparation;

[0053] Step two, add water and mix to get mixed tea material;

[0054] Step three, pile fermentation of mixed tea material, when the pile temperature reaches 50℃;

[0055] Step four, spread the mixed tea material after pile fermentation on the wooden board surface to dry;

[0056] Step five, steam steaming, the steam temperature during steaming is not less than 100℃;

[0057] Step six, molding by using mold;

[0058] Step seven, cool and age, and mulberry leaf and phellinus igniarius black tea is obtained.

[0059] <Experimental results>

[0060] 1、Fermentation process:

[0061] As Figure 2 shown, the fermentation process of mulberry leaf and phellinus igniarius black tea, the first row of each picture from left to right corresponds to CK group, group 1, group 2, the second row from left to right corresponds to group 3, group 4, group 5, and the fermentation time of the three pictures from left to right is 5d, 15d, 30d. During the fermentation process, the mulberry leaf and phellinus igniarius black tea is easy to soften, and the degree of decomposition is fast. With the increase of fermentation time and temperature, the tea leaves change from green to yellow-brown, and finally to transparent black. It smells faintly of fragrance and mushroom flavor.

[0062] 2、Sensory evaluation:

[0063] As Figure 3 shown, from left to right are CK group, group 1~5, and the mulberry leaf and phellinus igniarius black tea prepared by the above five groups is subjected to sensory evaluation. It has the unique black tea quality style of "red, thick, old, and mellow" of Liupao tea, and also has the fresh and mellow taste of mulberry leaf, and the "green smell" of mulberry leaf is removed.

[0064] Table 3 Sensory evaluation results

[0065]

[0066] 3、Physicochemical evaluation

[0067] After the fermentation of the black tea was finished, the product was detected for physical and chemical indexes, as shown in Table 4. The results showed that the contents of the ingredients in the black tea changed as the black tea aged to a certain time. The contents of the amino acids, tea polyphenols, theaflavins, thearubigins and theabrownins in the mulberry leaf and the mulberry mushroom black tea were higher than those in the control. It was indicated that the mulberry leaf and the mulberry mushroom black tea fermented after the addition of the mulberry leaf and the mulberry mushroom powder had a more mellow and sweet taste, a more transparent and bright color, and more rich ingredients. The product had the unique quality style of the black tea, such as red, thick, aged and mellow, and the superimposed effects of the health care functions of the mulberry mushroom powder, the mulberry leaf green tea and the green tea.

[0068] Table 4: Results of the detection of the physical and chemical indexes

[0069]

[0070] Evaluation of the immunity effect

[0071] 1. Experimental animals: SPF Kunming female mice, 6-8 weeks old, weighing 18-22 g.

[0072] 2. Reagents: cyclophosphamide (CTX), hydrochloric acid levamisole (Levamisole, LMS), mulberry leaf and mulberry mushroom black tea (SHC), decoction (STW), black tea (HC), mouse cytokine TNF-α detection kit, mouse cytokine IFN-γ detection kit, mouse cytokine IL-10 detection kit.

[0073] 3. Animal model:

[0074] The 60 mice were randomly divided into 6 groups (10 mice in each group), namely, the negative group (NC), the model group (MC), the positive drug group (PC), the mulberry leaf and mulberry mushroom black tea group (SHC), the decoction group (STW), the black tea (HC), and were bred in a sterile animal room (temperature: 23℃±2℃, humidity: 50%±5%, 12-hour light and dark cycle). All the mice could freely take the SPF grade drinking water and feed. The mulberry leaf and mulberry mushroom black tea group was the mulberry leaf and mulberry mushroom black tea powder obtained by soaking the mulberry leaf and mulberry mushroom black tea prepared according to the group formula 4 in 100℃ water for 1 h and then low-temperature concentration. The decoction group was the decoction powder obtained by low-temperature concentration after the raw materials were added with water and heated at 100℃ for 1 h. The black tea was prepared by reducing one of the monarch drugs, i.e. the momordica grosvenori, and one of the ministerial drugs, i.e. the polygonatum, according to the group formula 4, and using the same preparation method as the group formula 4. The black tea group (HC) was the black tea powder obtained by soaking the black tea prepared by the above method in 100℃ water for 1 h and then low-temperature concentration.

[0075] Adaptive feeding for 1 week, from the 8th day, the negative group of mice and the model group of mice were respectively given physiological saline by gavage at 0.1 mL / 10 g BW (body weight) per day; the positive drug group of mice was given LMS by gavage at 0.25 mg / kg BW per day; the mulberry leaf and black tea group of mice was given mulberry leaf and black tea powder (which needs to be prepared into a solution first) by gavage at 280 mg / kg BW per day; the decocting group of mice was given decocting powder (which needs to be prepared into a solution first) by gavage at 280 mg / kg BW per day; the black tea group of mice was given black tea powder (which needs to be prepared into a solution first) by gavage at 280 mg / kg BW per day; a total of 6 weeks of feeding, that is, 42 d, the body weight of the mice was recorded once every 3 d until the end. On the 27th, 28th, 29th, 30th, 31st, 33rd and 35th day of gavage treatment, the model group of mice, the positive drug group of mice, the mulberry leaf and black tea group of mice, the decocting group of mice and the black tea group of mice were respectively injected with 15 mg / kg BW of cyclophosphamide (CTX) by intraperitoneal injection, and the negative group of mice was injected with 0.1 mL / 10 g BW of physiological saline by intraperitoneal injection.

[0076] Feeding (after the last administration), the mice were fasted for 12 h without water, and the mice were sacrificed by cervical dislocation, the thymus and spleen were collected in a sterile centrifuge tube and stored at -80℃ for use. The detection of various immune indicators was carried out.

[0077] 4. Data recording and analysis:

[0078] After weighing, the mice were sacrificed, the thymus and spleen were dissected out and weighed, and the thymus coefficient and spleen coefficient were calculated.

[0079] Thymus coefficient = [thymus (mg) / body weight (g)] x 10;

[0080] Spleen coefficient = [spleen (mg) / body weight (g)] x 10.

[0081] Blood was collected from the medial canthus vein, placed at 4℃ overnight, and serum was separated. The levels of IFN-γ, TNF-α and IL-10 in the serum were detected by ELISA kit.

[0082] After the mice were killed, they were soaked in 75% alcohol for 5 min, and the spleen was isolated under sterile conditions. The spleen was cut and ground, and rinsed with sterilized PBS during the grinding process. The filtrate was filtered with a 200-mesh nylon screen, and centrifuged at 1200 r / min for 5 min. The supernatant was discarded, and 2 mL of red blood cell lysis solution was added to resuspend the cells. The cells were lysed at room temperature for 3 min, and centrifuged at 1200 r / min for 5 min. The supernatant was discarded, and the cells were washed twice with RPMI-1640 incomplete medium. The cells were resuspended in RPMI-1640 complete medium containing 10% FBS and 1% penicillin / streptomycin to obtain a spleen cell suspension. The concentration of the spleen cell suspension was adjusted to 10 7 The spleen cell suspension was inoculated into a 96-well plate at 100 μL per well, and 10 μL of ConA was added to each well to make the final concentration 5 μg / mL. Each group had 3 replicate wells, and the cells were induced to culture at 37°C in a 5% CO2 incubator for 16 h. Then, 20 μL of MTT was added to each well, and the incubation was continued for 4 h. The supernatant was discarded, 150 μL of DMSO was added, and the mixture was shaken at 37°C for 10 min. The OD value was determined at 490 nm using an enzyme-labeled instrument.

[0083] The cell proliferation rate (%) = (stimulated well OD value - control well OD value) x 100 / control well OD value.

[0084] Table 5: Mouse body weight data

[0085]

[0086] Table 6: Mouse organ coefficient data

[0087]

[0088] Table 7: IFN-γ, TNF-α and IL-10 content in mouse serum

[0089]

[0090] Table 8: Mouse spleen lymphocyte proliferation rate data

[0091]

[0092] Based on Table 5, it can be analyzed that the negative group mice drink and eat normally, and the body weight increases normally; the model group mice drink and eat relatively less, and the body weight increases slowly; and the positive drug group, mulberry leaf and sanghuang black tea group, decoction group mice drink and eat to a certain extent, and the body weight increases to a certain extent. The body weight of the negative group mice showed a stable growth trend; the model group mice were low in immunity due to the effect of cyclophosphamide, and the body weight increased slowly, and even showed a decrease in body weight; and compared with the model group, the body weight of the positive drug group, mulberry leaf and sanghuang black tea group, decoction group and black tea group increased significantly. It shows that mulberry leaf and sanghuang black tea, water extract and black tea can resist the slow growth of body weight caused by low immunity caused by cyclophosphamide to a certain extent, that is, it shows that mulberry leaf and sanghuang black tea, water extract and black tea may have a certain immunoregulatory effect on low immunity. The immunoregulatory effect of mulberry leaf and sanghuang black tea is better than that of the decoction group, which shows that the preparation process of mulberry leaf and sanghuang black tea has a positive effect on the compatibility of raw materials, and the immunoregulatory effect of mulberry leaf and sanghuang black tea is better than that of black tea, which shows that the compatibility of mulberry leaf and sanghuang black tea is better than that of black tea.

[0093] Based on Table 6, it can be analyzed that the immune system composed of immune organs, immune cells and immune active ingredients is the basis of the body's immunity. Thymus is the most important central immune organ of the body, and is the place where immune cells occur, differentiate and mature; the spleen is an important peripheral immune organ of the body, and is the place where mature T cells and B cells settle. Therefore, the increase of immune organs often represents the proliferation of lymphocytes, so as to directly reflect the enhancement of immune response. The strength of immune response of mice in each group was evaluated by determining the spleen coefficient and thymus coefficient of mice. Compared with the negative group mice, the spleen coefficient and thymus coefficient of the model group mice were significantly reduced; compared with the model group, the spleen coefficient and thymus coefficient of the positive drug group, mulberry leaf and sanghuang black tea group, decoction group and black tea group were significantly increased, which showed that mulberry leaf and sanghuang black tea and water extract could inhibit the atrophy of immune organs caused by low immunity to a certain extent. The inhibition of mulberry leaf and sanghuang black tea on the atrophy of immune organs caused by low immunity is better than that of the decoction group, which shows that the preparation process of mulberry leaf and sanghuang black tea has a positive effect on the compatibility of raw materials, and the inhibition of mulberry leaf and sanghuang black tea on the atrophy of immune organs caused by low immunity is better than that of black tea, which shows that the compatibility of mulberry leaf and sanghuang black tea is better than that of black tea.

[0094] Based on Table 7, it can be analyzed that immune cells and some non-immune cells can secrete some polypeptides, small molecule proteins or glycoproteins and other substances with extensive biological activities when stimulated, which are called cytokines. Cytokines include interleukins (IL), tumor necrosis factors (TNF), interferons (INF), growth factors (GF) and the like, which play a role in the body through autocrine and paracrine and the like, can specifically bind to the surface receptors of target cells, cause cell proliferation and differentiation, inflammatory response and immune response and the like, and enhance the resistance and clearance capacity of the body to external pathogens. The present application reflects the humoral and cellular immune states of the body by determining the contents of IFN-γ, TNF-α and IL-10 in serum. Compared with the negative group, the contents of IFN-γ, TNF-α and IL-10 in the serum of the model group mice are significantly reduced; compared with the model group, the content change levels of IFN-γ, TNF-α and IL-10 of the mice in the positive drug group, the mulberry leaf and mulberry mushroom black tea group, the decoction group and the black tea group are significantly improved; it shows that the mulberry leaf and mulberry mushroom black tea, the water extract and the black tea can effectively improve the immune capacity of immune cells, promote the secretion level of IFN-γ, TNF-α and IL-10 cytokines of immune damaged mice, and enhance the immune level of cells and humoral of mice. And the mulberry leaf and mulberry mushroom black tea group is better than the decoction group and the black tea group, which shows that the preparation process of the mulberry leaf and mulberry mushroom black tea has a positive effect on the compatibility effect of raw materials, and the compatibility effect of the mulberry leaf and mulberry mushroom black tea is better than that of the black tea.

[0095] Based on Table 8, it can be analyzed that the spleen is the largest peripheral lymphatic organ in the human and mammalian body, and the spleen is densely covered with lymphocytes, which is the main place for specific immunity of the body. Lymphocytes are an important component of the immune system, and the number often affects the strength of the body's immunity. Compared with the negative, the spleen cell proliferation rate of the model group mice is significantly reduced, which is due to the immunosuppressive effect of cyclophosphamide, leading to damage to the spleen of mice, growth and development being hindered, and cell viability being reduced. Compared with the model group, the spleen lymphocyte proliferation rates of the mice in the positive drug group, the mulberry leaf and mulberry mushroom black tea group, the decoction group and the black tea group are significantly improved, and the spleen lymphocyte proliferation rate of the mice in the mulberry leaf and mulberry mushroom black tea group reaches a level higher than that of the negative group, although there is no statistically significant difference, but it can be shown that the mulberry leaf and mulberry mushroom black tea, the water extract and the black tea can effectively promote the proliferation of spleen lymphocytes of immunosuppressed mice. The ability of the mulberry leaf and mulberry mushroom black tea to promote the proliferation of spleen lymphocytes of immunosuppressed mice is better than that of the decoction group and the black tea group, which shows that the preparation process of the mulberry leaf and mulberry mushroom black tea has a positive effect on the compatibility effect of raw materials, and the compatibility effect of the mulberry leaf and mulberry mushroom black tea is better than that of the black tea.

[0096] While embodiments of the application have been disclosed in connection with the above specification and drawings this description is not intended to limit the scope of the application and many modifications, enhancements, alternatives, and variations will become apparent to those skilled in the art from this disclosure. Accordingly, it is intended that the application not be limited to the described embodiments, but that it include all variations falling within the scope of the claims, and their equivalents.

Claims

1. A method for preparing mulberry leaf and phellinus igniarius black tea for enhancing immunity, characterized in that, The method comprises the following steps: Step one, prepare the following raw materials by weight: 100-150 parts of green tea, 45-65 parts of mulberry leaf green tea, 40-60 parts of sea buckthorn, 40-60 parts of momordica grosvenori, 3-8 parts of phellopteris griffithiana powder, 5-10 parts of lotus leaf, 5-8 parts of polygonatum, and 2-5 parts of gardenia; Step two, mix the raw materials of step one with water to obtain mixed tea materials; Step three, pile up the mixed tea materials for fermentation for 15-20 days, and turn the pile when the temperature of the pile reaches 50℃; Step four, dry; Step five, steam; Step six, press into shape using a mold; Step seven, cool and age for 40-50 days to obtain the mulberry and phellopteris black tea; The water content of the mixed tea materials in step two is 26-30%; The environmental temperature during the pile fermentation in step three is 40-50℃, and the environmental humidity is 85-95%.

2. The preparation method of mulberry leaf and mulberry sanghuang black tea as described in claim 1, characterized in that, The water content after drying in step four is not more than 13%.

3. The preparation method of mulberry leaf and mulberry sanghuang black tea as described in claim 1, characterized in that, The steam temperature during the steaming in step five is not less than 100℃, and the steaming time is 60-120 s.

4. A mulberry leaf and phellinus leaf black tea which is helpful for enhancing immunity, characterized in that, The mulberry and phellopteris black tea prepared by the preparation method of any one of claims 1-3.

5. The mulberry and phellopteris black tea of claim 4 for use in the preparation of a product for improving immunity.

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