Method for indoor seedling raising of quercus acutissima

Abstract

The application discloses a method for indoor seedling of Quercus acutissima, which comprises the following steps: (1) seed treatment; (2) germination; (3) sowing; (4) light environment setting; (5) dormancy induction; (6) dormancy release; and (7) light environment adjustment. Compared with the traditional seedling method of Quercus acutissima, the method shortens the dormancy period of Quercus acutissima and improves the seedling efficiency of Quercus acutissima.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of plant propagation technology, in particular to a method for indoor seedling of Quercus acutissima Carr. BACKGROUND

[0002] The dormant period is a period of temporary stop of growth and metabolism in the development process of plant body or its organs, is a beneficial biological characteristic, and is a biological adaptability to environmental conditions and seasonal changes obtained by plants through long-term evolution. If the plant cannot complete the normal natural dormancy, the next stage of growth and development will be hindered, and even if the conditions are suitable, it cannot germinate in time or germinate unevenly.

[0003] The photoperiod is an important signal for dormancy induction, and under the influence of this signal, many genes and proteins in the plant body undergo transformation from an active state to a dormant state. In dicotyledonous plants, the photoperiod is a key environmental signal for regulating bud dormancy. For example, poplar can be induced into dormancy by short day length; for perennial plants such as chestnut, styrax, and locust, the length of day is shorter than the critical day length, and the plants can be dormant. In addition, the change of hormones inside the bud is an internal factor affecting bud dormancy. Exogenous hormones are an effective way to affect the change of hormones inside the bud. In the last stage of endodormancy, the bud body is sensitive to chemical dormancy-breaking agents, and at this time, if suitable conditions are provided, the bud body can be released from dormancy and resume growth.

[0004] In the process of indoor seedling of Quercus acutissima Carr, the plant cannot enter the dormant period by itself due to the stable and unchanged environment, resulting in poor growth and development after the growth stagnation period. In the study of artificial dormancy induction, it is found that the application of exogenous ABA can promote the initiation of dormancy or delay the release of bud dormancy, and exogenous GA can induce the expression of β-1, 3-glucanase gene, promote the hydrolysis of the intercellular filament around the bud endoplasmic reticulum, and promote the release of bud dormancy. However, ABA and GA both play a role downstream of the photoperiod-mediated dormancy initiation pathway, and inhibition of GA biosynthesis under long-day conditions cannot induce plant dormancy. In view of this, the present application is proposed. SUMMARY

[0005] To solve the above-mentioned defects in the existing indoor seedling of Quercus acutissima Carr, the present application proposes a method for indoor seedling of Quercus acutissima Carr. By changing the photoperiod and exogenous hormone stimulation, the plant is induced into the dormant period, and the dormant period is broken in a short time, and the plant grows and develops normally.

[0006] The technical scheme of the present application is as follows: a method for indoor seedling of Quercus acutissima Carr, comprising seed treatment, germination, sowing, light environment setting, dormancy induction, dormancy release, and light environment adjustment.

[0007] (1) Seed treatment: remove impurities from the seeds of Quercus acutissima, remove diseased seeds, residual seeds, inferior seeds, and retain full and mature seeds;

[0008] (2) Germination: 7-15 days before sowing, the selected seeds are broken and soaked in a mixed solution of 250 mg / L gibberellin and 20 mg / L auxin for 2-3 days. When the amount of cracked seeds accounts for 30% of the total seeds, they are taken out for standby;

[0009] (3) Sowing and seedling raising: sow the seeds in fully moistened soil, with the seeds 2-3 cm from the soil surface, and place them in a seedling raising space for seedling raising;

[0010] (4) Light environment setting: during the seedling raising period, the light quality, intensity and photoperiod of red, blue and far-red LED beads in the plant growth lamp above the soil are adjusted by the light control system;

[0011] (5) Dormancy induction: when Quercus acutissima enters the growth stagnation period, short-day regulation and exogenous abscisic acid treatment are used to artificially induce Quercus acutissima into dormancy;

[0012] (6) Dormancy release: when Quercus acutissima enters the deep dormancy stage, long-day regulation and exogenous gibberellin treatment are used to artificially induce Quercus acutissima to release dormancy;

[0013] (7) Adjusting the light environment: after Quercus acutissima releases dormancy and new shoots germinate, the light quality, intensity and photoperiod of red, blue and far-red LED beads in the plant growth lamp are controlled again by the light control system to maintain a day / night light duration of 12h / 12h.

[0014] Further in step (3), the sowing method is horizontal sowing.

[0015] Further in step (4), the light control system controls the red, blue and far-red LED beads, which are opened in different modes at different times, and the specific steps are as follows:

[0016] 1) Turn on the red and blue LED beads, the red LED bead selects a wavelength of 620 nm and a light intensity of 70.8 μmol / s / m 2 , the blue LED bead selects a wavelength of 420 nm and a light intensity of 27.2 μmol / s / m 2 , and irradiates for 60 min;

[0017] 2) Adjust the red light intensity to 141.6 μmol / s / m 2 , and the blue light intensity to 54.4 μmol / s / m 2 , and irradiate for 10h;

[0018] 3) Red light intensity is adjusted to 70.8 μmol / s / m 2 , blue light intensity is adjusted to 27.2 μmol / s / m 2 , irradiation for 50 min;

[0019] 4) Turn on the far-red LED lamp bead, select the wavelength of 730 nm, the far-red light intensity is 17.8 μmol / s / m 2 , red light intensity is 57.9 μmol / s / m 2 , blue light intensity is 22.3 μmol / s / m 2 , irradiation for 10 min, and enter the dark period after the end.

[0020] Further, in the step (5), when there is no new bud germination in Quercus sessilifolia for 5 consecutive days, it is the growth stagnation period.

[0021] Further, in the step (5), the short-day treatment keeps the day / night light duration at 8h / 16h, and the specific regulation method is:

[0022] 1) Turn on the red and blue LED lamp beads, select the wavelength of the red LED lamp bead to be 620 nm, and the light intensity to be 70.8 μmol / s / m 2 , select the wavelength of the blue LED lamp bead to be 420 nm, and the light intensity to be 27.2 μmol / s / m 2 , irradiation for 60 min;

[0023] 2) Adjust the red light intensity to 141.6 μmol / s / m 2 , and the blue light intensity to 54.4 μmol / s / m 2 , irradiation for 6h;

[0024] 3) Adjust the red light intensity to 70.8 μmol / s / m 2 , and the blue light intensity to 27.2 μmol / s / m 2 , irradiation for 60 min, and enter the dark period after the end.

[0025] Further, in the step (5), the exogenous abscisic acid concentration is 100 μmol / L, which is uniformly sprayed on the branches and leaves of Quercus sessilifolia every 24h, and the spraying is stopped when Quercus sessilifolia enters the deep dormancy stage.

[0026] Further, in the step (6), when the sucrose content of Quercus sessilifolia remains at a stable level, Quercus sessilifolia enters deep dormancy, and artificial dormancy release is induced after 5 days.

[0027] Further, in the step (6), the long-day treatment keeps the day / night light duration at 16h / 8h, and the specific regulation method is:

[0028] 1) Turn on red and blue LED beads, red LED beads select wavelength of 620 nm, light intensity of 70.8 μmol / s / m 2 , blue LED beads select wavelength of 420 nm, light intensity of 27.2 μmol / s / m 2 , irradiation for 60 min;

[0029] 2) Adjust the red light intensity to 141.6 μmol / s / m 2 , blue light intensity to 54.4 μmol / s / m 2 , irradiation for 14 h;

[0030] 3) Adjust the red light intensity to 70.8 μmol / s / m 2 , blue light intensity to 27.2 μmol / s / m 2 , irradiation for 50 min;

[0031] 4) Turn on far-red LED beads, select wavelength of 730 nm, far-red light intensity of 17.8 μmol / s / m 2 , red light intensity of 57.9 μmol / s / m 2 , blue light intensity of 22.3 μmol / s / m 2 , irradiation for 10 min, and then enter the dark period.

[0032] Further, in the step (7), the specific steps of adjusting the light environment are:

[0033] 1) Turn on red and blue LED beads, red LED beads select wavelength of 620 nm, light intensity of 70.8 μmol / s / m 2 , blue LED beads select wavelength of 420 nm, light intensity of 27.2 μmol / s / m 2 , irradiation for 60 min;

[0034] 2) Adjust the red light intensity to 141.6 μmol / s / m 2 , blue light intensity to 54.4 μmol / s / m 2 , irradiation for 10 h;

[0035] 3) Adjust the red light intensity to 70.8 μmol / s / m 2 , blue light intensity to 27.2 μmol / s / m 2 , irradiation for 50 min;

[0036] 4) Turn on far-red LED beads, select wavelength of 730 nm, far-red light intensity of 17.8 μmol / s / m 2 , red light intensity of 57.9 μmol / s / m2 Blue light intensity: 22.3 μmol / s / m 2 Irradiation time: 10 min, and then enter the dark period.

[0037] The present application has the advantages of:

[0038] The indoor seedling raising method of Quercus acutissima provided by the present application aims at the problems in the existing indoor seedling raising process of Quercus acutissima, such as the inability of Quercus acutissima to enter dormancy and end dormancy autonomously, growth stagnation, and poor growth and development, etc. By artificially controlling the light environment, creating natural light cycle changes, and combining with exogenous abscisic acid and exogenous gibberellin spraying, the oak plant is induced to enter and end dormancy. On the basis of the existing technology, the growth stagnation period of Quercus acutissima is broken, the dormancy time of Quercus acutissima is shortened while meeting the dormancy demand of Quercus acutissima, and the problem of the inability of Quercus acutissima to enter and end dormancy autonomously caused by indoor seedling raising is overcome, so as to enable Quercus acutissima to grow normally, and provide technical support for indoor seedling raising of Quercus acutissima. DETAILED DESCRIPTION

[0039] The technical solutions of the present application are further explained and described below through specific examples. EMBODIMENT

[0040] First, remove impurities from Quercus acutissima plant seeds, remove diseased seeds, residual seeds, and inferior seeds, and retain high-quality mature seeds with full grains. About 7-15 days before sowing, the selected seeds are broken and soaked in a mixed solution of 250 mg / L gibberellin and 20 mg / L auxin for 2-3 days. When the seeds have 30% split mouths, the seeds are sown horizontally in fully moistened soil matrix and buried at a distance of 2-3 cm from the soil surface. After sowing, the Quercus acutissima seedlings are placed in the seedling raising space, and the plant growth lamp is set above the soil. The light quality, light intensity, and light cycle of the red light, blue light, and far-red light LED beads in the plant growth lamp are controlled by the light control system to irradiate the Quercus acutissima seedlings for 12 hours per day. The specific steps are as follows:

[0041] 1) Turn on the red light and blue light LED beads. The red light LED beads have a wavelength of 620 nm and a light intensity of 70.8 μmol / s / m 2 The blue light LED beads have a wavelength of 420 nm and a light intensity of 27.2 μmol / s / m 2 Irradiation time: 60 min;

[0042] 2) Adjust the red light intensity to 141.6 μmol / s / m 2 Adjust the blue light intensity to 54.4 μmol / s / m 2 Irradiation time: 10 h;

[0043] 3) Adjust the red light intensity to 70.8 μmol / s / m 2, blue light intensity was adjusted to 27.2 μmol / s / m 2 , irradiation for 50 min;

[0044] 4) Turn on the far-red LED lamp bead, select the wavelength of 730 nm, the far-red light intensity is 17.8 μmol / s / m 2 , red light intensity is 57.9 μmol / s / m 2 , blue light intensity is 22.3 μmol / s / m 2 , irradiation for 10 min, and then enter the dark period.

[0045] In addition, proper water and fertilizer management is needed during seedling raising. When the oak has no new bud germination for 5 consecutive days, it enters the growth stagnation period. At this time, adjust the light period, keep the day / night light length as 8h / 16h, and the specific steps are as follows:

[0046] 1) Turn on the red and blue LED lamp beads, the red LED lamp bead selects the wavelength of 620 nm, and the light intensity is 70.8 μmol / s / m 2 , the blue LED lamp bead selects the wavelength of 420 nm, and the light intensity is 27.2 μmol / s / m 2 , irradiation for 60 min;

[0047] 2) Adjust the red light intensity to 141.6 μmol / s / m 2 , blue light intensity is adjusted to 54.4 μmol / s / m 2 , irradiation for 6h;

[0048] 3) Adjust the red light intensity to 70.8 μmol / s / m 2 , blue light intensity is adjusted to 27.2 μmol / s / m 2 , irradiation for 60 min, and then enter the dark period.

[0049] At the same time, 100 μmol / L abscisic acid is sprayed on the branches and leaves of oak every day. The sucrose content is detected every 5 days. With the advancement of oak plant dormancy, the sucrose content increases continuously. When the oak enters the deep dormancy stage, the sucrose content remains at a stable level. At this time, immediately stop spraying exogenous abscisic acid. After determining that the oak has entered the deep dormancy, monitor the abscisic acid content in the oak plant. When the content stops rising, adjust the day / night light length to 16h / 8h, and the specific steps are as follows:

[0050] 1) Turn on the red and blue LED lamp beads, the red LED lamp bead selects the wavelength of 620 nm, and the light intensity is 70.8 μmol / s / m 2The blue LED beads are selected with a wavelength of 420nm and a light intensity of 27.2 μmol / s / m. 2 Irradiate for 60 minutes;

[0051] 2) Adjust the red light intensity to 141.6 μmol / s / m 2 The blue light intensity was adjusted to 54.4 μmol / s / m 2 Irradiate for 14 hours;

[0052] 3) Adjust the red light intensity to 70.8 μmol / s / m 2 The blue light intensity was adjusted to 27.2 μmol / s / m 2 Irradiate for 50 minutes;

[0053] 4) Turn on the far-infrared LED beads, select a wavelength of 730nm, and the far-infrared light intensity is 17.8μmol / s / m. 2 The intensity of the red light is 57.9 μmol / s / m. 2 The blue light intensity is 22.3 μmol / s / m. 2 Irradiate for 10 minutes, then enter the dark period.

[0054] Simultaneously, a 100 mol / L gibberellin solution is sprayed evenly onto the branches and leaves of the Quercus acutissima daily. After the new shoots of the Quercus acutissima sprout, the spraying of exogenous gibberellin is stopped, and the light quality, light intensity, and photoperiod of the plant growth lamp are controlled through a light control system to maintain a day / night light duration of 12h / 12h. The specific steps are as follows:

[0055] 1) Turn on the red and blue LED beads. The red LED bead should have a wavelength of 620 nm and a light intensity of 70.8 μmol / s / m. 2 The blue LED beads have a wavelength of 420 nm and a light intensity of 27.2 μmol / s / m. 2 , irradiate for 60 minutes;

[0056] 2) Adjust the red light intensity to 141.6 μmol / s / m 2 The blue light intensity was adjusted to 54.4 μmol / s / m 2 Irradiate for 10 hours;

[0057] 3) Adjust the red light intensity to 70.8 μmol / s / m 2 The blue light intensity was adjusted to 27.2 μmol / s / m 2 Irradiate for 50 minutes;

[0058] 4) Turn on the far-infrared LED beads, select a wavelength of 730 nm, and the far-infrared light intensity is 17.8 μmol / s / m. 2, the red light intensity is 57.9 μmol / s / m 2 , the blue light intensity is 22.3 μmol / s / m 2 , irradiation for 10 min, and then entering the dark period.

[0059] Comparative Example 1

[0060] The same parts of the comparative example and the example are not repeated, and the difference is that the oak in the comparative example is not sprayed with exogenous hormones abscisic acid and gibberellin. Only the length of daily light is adjusted at different periods.

[0061] Comparative Example 2

[0062] The same parts of the comparative example and the example are not repeated, and the difference is that the oak in the comparative example is not adjusted by photoperiod, and after sowing, the length of daily light is always kept at 12h. Only the spraying of exogenous hormones abscisic acid and gibberellin is performed at different periods.

[0063] Comparative Example 3

[0064] The same parts of the comparative example and the example are not repeated, and the difference is that the oak in the comparative example is not adjusted by photoperiod, and after sowing, the length of daily light is always kept at 12h. No spraying of exogenous abscisic acid and gibberellin.

[0065] The oak plants in the examples and comparative examples 1-3 are tested for dormancy induction, and the test results are shown in Table 1, where T1 is the time required for the oak to grow from germination to the growth stagnation period; T2 is the time required for the oak to enter deep dormancy; and T3 is the time required for the oak to remove dormancy:

[0066]

[0067] The sucrose and abscisic acid contents of the oak are monitored every 5 days after the oak enters the stagnation period, and the specific experimental results are shown in Table 2, where "-" indicates that no monitoring is performed:

[0068]

[0069] The dry weight and leaf area of the oak are measured every 3 days after the oak removes the dormancy period, and the specific experimental results are shown in Table 3, where "-" indicates that no monitoring is performed:

[0070]

[0071] As can be seen from the above, the technical scheme of the present application adopts photoperiod regulation and exogenous hormone treatment for oak, which can induce the oak to enter and remove the dormancy period, effectively shortens the dormancy period, and makes the oak grow healthily.

[0072] The above merely illustrates the specific embodiments of the present application, but the protection scope of the present application is not limited thereto, any person skilled in the art can easily think of the changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A method for raising Quercus acutissima seedlings in a greenhouse, characterized in that: The method comprises the following steps: (1) Seed treatment: remove impurities from the seed of Quercus acutissima, remove diseased seeds, residual seeds, and inferior seeds, and retain full and mature seeds; (2) Germination: 7-15 days before sowing, the selected seeds are broken and soaked in a mixed solution of 250 mg / L gibberellin and 20 mg / L auxin for 2-3 days, and when the amount of seeds with cracked mouths accounts for 30% of the total amount of seeds, the seeds are taken out for standby; (3) Sowing and seedling raising: the seeds are sown in fully moistened soil, the seeds are 2-3 cm away from the soil surface, and the seeds are placed in a seedling raising space for seedling raising; (4) Light environment setting: during the seedling raising period, the light quality, light intensity and photoperiod of the red light, blue light and far-red light LED beads in the plant growth lamp above the soil are adjusted through a light control system; The light control system controls the red light, blue light and far-red light LED beads, and different modes are opened in time and in sections, and the steps are as follows: 1) Turn on red and blue light LED lamp beads, red light LED lamp beads select wavelength of 620 nm, light intensity of 70.8 μmol / s / m 2 , blue light LED lamp beads select wavelength of 420 nm, light intensity of 27.2 μmol / s / m 2 , irradiation for 60 min; 2) Red light intensity adjusted to 141.6 μmol / s / m 2 , blue light intensity adjusted to 54.4 μmol / s / m 2 , for 10 h; 3) Red light intensity adjusted to 70.8 pmol / s / m 2 , blue light intensity adjusted to 27.2 pmol / s / m 2 , for 50 min; 4) Turn on the far-red LED lamp bead, select the wavelength of 730 nm, the far-red light intensity is 17.8 μmol / s / m 2 , the red light intensity is 57.9 μmol / s / m 2 , the blue light intensity is 22.3 μmol / s / m 2 , irradiate for 10 min, and enter the dark period after the end; (5) Dormancy induction: after Quercus acutissima enters the growth stagnation period, short-day regulation and exogenous abscisic acid treatment are adopted, the exogenous abscisic acid concentration is 100 μmol / L, and the exogenous abscisic acid is uniformly sprayed on the branches and leaves of Quercus acutissima every 24 h, and the spraying is stopped when Quercus acutissima enters the deep dormancy stage; Quercus acutissima is artificially induced into the dormancy period; when no new buds germinate in Quercus acutissima for 5 consecutive days, it is the growth stagnation period; The specific regulation method is that the short-day treatment keeps the day / night light duration at 8h / 16h; 1) Turn on red and blue light LED beads, red light LED beads select wavelength of 620 nm, light intensity of 70.8 μmol / s / m 2 , blue light LED beads select wavelength of 420 nm, light intensity of 27.2 μmol / s / m 2 , irradiation for 60 min; 2) Red light intensity adjusted to 141.6 μmol / s / m 2 , blue light intensity adjusted to 54.4 μmol / s / m 2 , for 6h; 3) Red light intensity adjusted to 70.8 pmol / s / m 2 , blue light intensity adjusted to 27.2 pmol / s / m 2 , irradiation for 60 min, after which a dark period was entered; (6) Dormancy release: after Quercus acutissima enters the deep dormancy stage, long-day regulation and exogenous gibberellin treatment are adopted, and Quercus acutissima is artificially induced to release dormancy; when the sucrose content of Quercus acutissima remains stable, Quercus acutissima enters deep dormancy, and Quercus acutissima is artificially induced to release dormancy after 5 days; The specific regulation method is that the long-day treatment keeps the day / night light duration at 16h / 8h; 1) Turn on red and blue light LED beads, red light LED beads select wavelength of 620 nm, light intensity of 70.8 μmol / s / m 2 , blue light LED beads select wavelength of 420 nm, light intensity of 27.2 μmol / s / m 2 , irradiation for 60 min; 2) Red light intensity adjusted to 141.6 μmol / s / m 2 , blue light intensity adjusted to 54.4 μmol / s / m 2 , for 14 h; 3) Red light intensity adjusted to 70.8 pmol / s / m 2 , blue light intensity adjusted to 27.2 pmol / s / m 2 , for 50 min; 4) Turn on the far-red LED lamp bead, select the wavelength of 730 nm, the far-red light intensity is 17.8 μmol / s / m 2 , the red light intensity is 57.9 μmol / s / m 2 , the blue light intensity is 22.3 μmol / s / m 2 , irradiate for 10 min, and enter the dark period after the end; (7) Adjustment of light environment: after Quercus acutissima releases dormancy and new buds germinate, the light quality, light intensity and photoperiod of the red light, blue light and far-red light LED beads in the plant growth lamp are controlled again through the light control system, the day / night light duration is kept at 12h / 12h, and the light control system controls the red light, blue light and far-red light LED beads, and different modes are opened in time and in sections, which is the same as step (4).

2. The method of growing Quercus infectoria in a room according to claim 1, characterized by: In step (3), the seed sowing method is horizontal sowing.

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