Immunoassay

Abstract

The present application relates to an immunoassay method comprising the following steps: a magnetic collection component carries an empty reactor and moves it to a liquid transfer station, and the reactor is moved out of the magnetic collection component by a gripper to mix the liquid in the reactor; the mixed reactor is incubated; the magnetic beads in the reactor are collected on the inner wall of the reactor, and waste liquid is absorbed by a sampling piece; the magnetic collection component carries the reactor after absorbing waste liquid and moves it to a liquid injection station, and a cleaning liquid is injected into the reactor, and the reactor is moved out of the magnetic collection component by a gripper to mix the liquid in the reactor; the reactor containing the cleaning liquid is moved into the magnetic collection component by a gripper and moved to the liquid transfer station, and waste liquid is absorbed by a sampling piece; the magnetic collection component carries the reactor and moves it to the liquid injection station, and a substrate liquid is injected into the reactor; the reactor containing the substrate liquid is transferred to a measuring mechanism by a gripper for measurement. In this way, the manufacturing cost and volume of the immunoassay analyzer can be reduced.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to an immunoassay method. Background Art

[0002] Immunoassay analyzers have been increasingly widely used in recent years due to their advantages such as high sensitivity, good specificity, wide linear range, high throughput, strong platform expandability, and rich menu of test items. However, for traditional immunoassay methods, despite their high throughput, the immunoassay analyzers that perform the immunoassay methods have the disadvantages of high price, large size, and high professional requirements for operation, making the immunoassay methods unsuitable for use in small and medium-sized laboratories or medical institutions, thus limiting the availability of immunoassay methods. Summary of the invention

[0003] A technical problem solved by the present application is how to reduce the manufacturing cost and volume of an immunoassay analyzer that performs an immunoassay method.

[0004] An immunoassay method comprising:

[0005] The first direction, the second direction and the third direction are arranged at an angle to each other, the sampling member and the gripper make linear motion along the first direction and the third direction, the carrying assembly for holding the sample and the reagent and the input member for carrying the reactor make linear motion along the second direction, and the magnetic collection assembly carries the reactor to make linear motion between the pipetting station and the injection station arranged at intervals along the second direction; the immunoassay method comprises the following steps:

[0006] The empty reactor on the input member is transferred to the magnetic collection assembly by a gripper, the magnetic collection assembly carries the empty reactor and moves to the liquid transfer station, the reaction liquid is sucked from the carrying assembly by the sampling member and injected into the reactor, and the reactor is moved out of the magnetic collection assembly by the gripper to mix the liquid in the reactor;

[0007] Incubate the mixed reactor;

[0008] The incubated reactor is moved into the magnetic collection assembly and moved to the liquid transfer station by the gripper, the magnetic beads in the reactor are collected on the inner wall of the reactor, and the waste liquid is sucked by the sampling piece;

[0009] The magnetic collection assembly carries the reactor after absorbing the waste liquid and moves it to the liquid injection station, the cleaning liquid is injected into the reactor through the first liquid injection assembly, and the reactor is moved out of the magnetic collection assembly by a gripper to mix the liquid in the reactor;

[0010] The reactor containing the cleaning liquid is moved into the magnetic collection assembly by the gripper and moved to the liquid transfer station, and the waste liquid is sucked by the sampling member;

[0011] The magnetic collection assembly carrying the reactor is moved to the liquid injection station, and the substrate liquid is injected into the reactor through the second liquid injection assembly;

[0012] Using a gripper, the reactor containing the substrate liquid is moved out of the magnetic collection assembly to mix the liquid in the reactor; and

[0013] The reactor containing the substrate liquid is transferred to the measuring mechanism for measurement by the gripper.

[0014] In one embodiment, the reactor is carried by an incubation mechanism capable of linear motion along the first direction for incubation.

[0015] In one embodiment, the liquid in the reactor is mixed by the same mixing mechanism.

[0016] In one embodiment, after the sampling component is injected with reaction liquid or absorbs waste liquid, the sampling component is cleaned.

[0017] In one embodiment, the step of injecting a cleaning solution into the reactor after the reaction solution has been completely absorbed and then absorbing the waste liquid is recorded as a first-stage cleaning and separation process, and the immunoassay method includes multiple stages of the cleaning and separation process.

[0018] In one embodiment, the number of the injection stations is two, and the two injection stations are respectively recorded as the first injection station and the second injection station, and the cleaning liquid is injected into the reactor at the first injection station; after the reactor absorbs the waste liquid for the last time, the substrate liquid is injected into the reactor at the second injection station.

[0019] In one embodiment, before the reactor containing the reaction liquid is used to absorb the waste liquid, the reactor is moved to the liquid injection station to inject the cleaning liquid.

[0020] In one embodiment, between injecting the substrate solution into the reactor and performing the measurement, the reactor is moved out of the magnetic collection assembly by a gripper for incubation.

[0021] In one embodiment, the magnetic collection component has a loading position and a collection position for supporting the reactor, the loading position and the collection position are arranged at intervals along the movement direction of the magnetic collection component, the loading position can move to the pipetting station, and the collection position can move to the pipetting station or the injection station.

[0022] In one embodiment, there are multiple collection positions. For two adjacent collection positions, the reactor is injected with cleaning liquid at one of the collection positions and then transferred to the other collection position to absorb waste liquid.

[0023] A technical effect of an embodiment of the present application is: considering that the sampling piece can make two-dimensional linear motion along the first direction and the third direction, and the carrying component makes linear motion along the second direction, the sampling piece makes three-dimensional linear motion relative to the carrying component, ensuring that the sampling piece can smoothly absorb liquid from the carrying component respectively. And the gripper can make two-dimensional linear motion along the first direction and the third direction, and the input piece makes linear motion along the second direction, so that the gripper makes three-dimensional linear motion relative to the input piece, ensuring that the gripper can smoothly grab the reactor from the input piece. At the same time, the magnetic collection component carries the reactor to make linear motion between the pipetting station and the injection station spaced apart along the second direction. The immunoassay method formed on this basis can simplify the structure of the immunoassay analyzer that performs the method, thereby reducing the manufacturing cost and volume of the immunoassay analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The figure is a schematic diagram of the three-dimensional structure of an immunoassay analyzer provided in one embodiment.

[0025] Figure 2 for Figure 1 The three-dimensional structure diagram of the immunoassay analyzer after removing the chassis is shown.

[0026] Figure 3 for Figure 1 The schematic diagram of the immunoassay analyzer from top view after some structures are removed.

[0027] Figure 4 for Figure 1 Schematic diagram of the partial three-dimensional structure of the immunoassay analyzer shown.

[0028] Figure 5 for Figure 1 Schematic diagram of the three-dimensional structure of the magnetic collection component in the immunoassay analyzer shown.

[0029] Figure 6 A process flow chart of an immunoassay method provided in one embodiment.

[0030] Reference numerals: immunoanalyzer 10, reactor 20, input mechanism 100, input member 110, consumables mechanism 200, sample tube 210, reagent kit 220, sliding member 230, belt conveyor assembly 240, sampling mechanism 300, sampling member 310, first sampling drive module 321, second sampling drive module 322, transport mechanism 400, gripper 410, first transport drive module 421, second transport drive module 422, incubation mechanism 500, magnetic collection mechanism 600, pipetting station 610, injection station 611. 20. The first liquid injection station 621, the second liquid injection station 622, the transfer station 630, the magnetic collection component 640, the carrier 641, the groove 6411, the magnetic component 642, the collection position 643, the first collection position 6431, the second collection position 6432, the third collection position 6433, the fourth collection position 6434, the sample adding position 644, the belt drive assembly 650, the liquid injection mechanism 700, the first liquid injection component 710, the second liquid injection component 720, the mixing mechanism 810, the measuring mechanism 820, and the chassis 830. DETAILED DESCRIPTION

[0031] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0032] In the description of the present application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0033] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, if the term "plurality" appears, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0034] In this application, unless otherwise clearly specified and limited, if the terms "installed", "connected", "connected", "fixed" and the like appear, these terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0035] In the present application, unless otherwise clearly specified and limited, if there is a description that a first feature is "above" or "below" a second feature, etc., or similar descriptions appear, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0036] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are for illustrative purposes only and do not represent the only implementation method.

[0037] See 1. Figure 2 and Figure 3 An immunoassay analyzer 10 provided in one embodiment of the present application includes an input mechanism 100, a consumable mechanism 200, a sampling mechanism 300, a transport mechanism 400, an incubation mechanism 500, a magnetic collection mechanism 600, a liquid injection mechanism 700, a mixing mechanism 810, a measuring mechanism 820, and a chassis 830. The chassis 830 can be used as a storage carrier, so that the input mechanism 100, the consumable mechanism 200, the sampling mechanism 300, the incubation mechanism 500, the transport mechanism 400, the magnetic collection mechanism 600, the liquid injection mechanism 700, the mixing mechanism 810, and the measuring mechanism 820 can all be accommodated in the chassis 830.

[0038] The first direction, the second direction and the third direction are three different directions, and the first direction, the second direction and the third direction can be arranged at an angle in pairs, for example, the first direction, the second direction and the third direction can be perpendicular to each other in pairs, so that the first direction, the second direction and the third direction can be the extension directions of the three coordinate axes in the spatial rectangular coordinate system, for example, the first direction can be the X-axis direction, the second direction can be the opposite direction of the Y-axis, and the third direction can be the Z-axis direction. The gravity direction of the immunoanalyzer 10 during operation is the third direction.

[0039] See 1. Figure 2 , Figure 3 and Figure 4 In some embodiments, the input mechanism 100 includes an input member 110, which is used to transport and carry an empty and clean reactor 20. The input member 110 is provided with a plurality of insertion holes, and the reactor 20 is inserted into the insertion holes, so that the input member 110 can achieve a buffering function for the reactor 20. The input member 110 can perform linear motion along the second direction, so that the input member 110 carries the reactor 20 to perform reciprocating linear motion in the second direction.

[0040] See 1. Figure 2 , Figure 3 and Figure 4 In some embodiments, the consumable mechanism 200 includes a sample rack and a reagent box 220. The sample rack can be slidably arranged along the second direction relative to the chassis 830, so that the sample rack can make reciprocating linear motion along the second direction. A plurality of sample tubes 210 are inserted on the sample rack, and the sample tubes 210 can contain samples to be tested. The reagent box 220 can also be slidably arranged along the second direction relative to the chassis 830, so that the reagent box 220 can make reciprocating linear motion along the second direction, and the reagent box 220 contains reagents. The sample tubes 210 and the reagent box 220 can be collectively referred to as a carrying assembly. In other words, the carrying assembly includes the sample tubes 210 and the reagent box 220.

[0041] The consumables mechanism 200 may further include a sliding member 230 and a belt conveyor assembly 240. The sliding member 230 is slidably disposed on the chassis 830 so that the sliding member 230 can perform reciprocating linear motion relative to the chassis 830 along the second direction. The sample tube 210 and the reagent box 220 may be fixed on the sliding member 230, so the sample tube 210 and the reagent box 220 can follow the sliding member 230 to perform reciprocating linear motion relative to the chassis 830 along the second direction. The belt conveyor assembly 240 is used to drive the sliding member 230 to perform linear motion along the second direction. The input member 110 may also be disposed on the sliding member 230 so that the bearing assembly formed by the sample tube 210 and the reagent box 220 is spaced apart from the input member 110 along the second direction on the sliding member 230. Therefore, by the sliding member 230 performing linear motion along the second direction, the input member 110, the sample tube 210 and the reagent box 220 can synchronously follow the sliding member 230 to perform linear motion along the second direction. Since the power for the linear motion of the input part 110, the sample tube 210 and the reagent box 220 comes from the same belt conveyor assembly 240, that is, the motion of the input part 110, the sample tube 210 and the reagent box 220 can share the same belt conveyor assembly 240, it is prevented that the input part 110, the sample tube 210 and the reagent box 220 are driven by different belt conveyor assemblies 240 respectively. In this way, the structure of the immunoassay analyzer 10 can be simplified and the manufacturing cost and volume of the immunoassay analyzer 10 can be reduced.

[0042] See 1. Figure 2 , Figure 3 and Figure 4 In some embodiments, the sampling mechanism 300 may include a sampling member 310, a first sampling drive module 321, and a second sampling drive module 322. The first sampling drive module 321 may extend along the first direction, and the second sampling drive module 322 may extend along the third direction. The second sampling drive module 322 may be slidably connected to the first sampling drive module, and the first sampling drive module 321 is used to drive the second sampling drive module 322 to slide back and forth along the first direction. The sampling member 310 is slidably disposed on the second sampling drive module 322, and the second sampling drive module 322 is used to drive the sampling member 310 to slide back and forth along the third direction. The sampling member 310 may be a needle-shaped structure, etc. Therefore, when the first sampling drive module 321 and the second sampling drive module 322 move, the sampling member 310 can be made to move linearly relative to the chassis 830 along the first direction and the third direction, so that the sampling member 310 has two degrees of freedom of linear motion, thereby realizing the two-dimensional motion of the sampling member 310.

[0043] By making the sampling member 310 perform linear motion with two degrees of freedom, the sampling member 310 can perform two-dimensional motion. Meanwhile, the sliding member 230 can drive the sample tube 210 and the reagent box 220 to perform linear motion along the second direction. Therefore, the sampling member 310 has three degrees of freedom relative to the sample tube 210 and the reagent box 220, that is, the sampling member 310 can perform three-dimensional motion relative to the sample tube 210 and the reagent box 220. In this way, the sampling member 310 can absorb samples in different sample tubes 210 and reagents in different reagent boxes 220. Since the power component for the sampling member 310 to perform linear motion relative to the sample tube 210 and the reagent box 220 along the second direction is arranged on the consumable mechanism 200, it is possible to avoid the power component for driving the sampling member 310 to perform linear motion relative to the sample tube 210 and the reagent box 220 along the second direction being arranged on the sampling mechanism 300, thereby reducing the weight of the sampling mechanism 300, thereby reducing the vibration and deflection generated by the sampling member 310 during the motion process, and improving the motion accuracy of the sampling member 310. Since the sampling member 310 can absorb the sample in the sample tube 210 and the reagent in the reagent box 220 , the sample and the reagent can be absorbed by the same sampling member 310 .

[0044] In some embodiments, the immunoassay analyzer 10 may further include a cleaning pool. Since the reagent and the sample are sucked through the same sampling member 310, in order to avoid cross contamination, after the sampling member 310 has finished sucking the sample, the sampling member 310 may be moved to the cleaning pool for cleaning. When the sampling member 310 sucks the reagent, the sample adhering to the sampling member 310 may be prevented from contaminating the reagent. Similarly, after the sampling member 310 has finished sucking the reagent, the sampling member 310 may be moved to the cleaning pool for cleaning. When the sampling member 310 sucks the sample, the reagent adhering to the sampling member 310 may be prevented from contaminating the sample.

[0045] See 1. Figure 2 , Figure 3 and Figure 4In some embodiments, the transfer mechanism 400 may include a gripper 410, a first transfer drive module 421, and a second transfer drive module 422. The first transfer drive module 421 may extend in a first direction, and the second transfer drive module 422 may extend in a third direction. The second transfer drive module 422 may be slidably connected to the first transfer drive module, and the first transfer drive module 421 is used to drive the second transfer drive module 422 to slide back and forth in the first direction. The gripper 410 is slidably arranged on the second transfer drive module 422, and the second transfer drive module 422 is used to drive the gripper 410 to slide back and forth in the third direction. Therefore, when the first transfer drive module 421 and the second transfer drive module 422 move, the gripper 410 can be made to move linearly relative to the chassis 830 in the first direction and the third direction, so that the gripper 410 has two degrees of freedom of linear motion, thereby realizing the two-dimensional motion of the gripper 410. In other embodiments, two second transfer drive modules 422 may be disposed on the first transfer drive module 421 . Obviously, one gripper 410 may be disposed on each second transfer drive module 422 .

[0046] By making the gripper 410 perform linear motion with two degrees of freedom, the gripper 410 can perform two-dimensional motion. Meanwhile, the sliding member 230 can drive the input member 110 to perform linear motion along the second direction. Therefore, the gripper 410 has three degrees of freedom relative to the input member 110, that is, the gripper 410 can perform three-dimensional motion relative to the input member 110, so that the gripper 410 can grasp the reactor 20 at different positions in the input member 110. Since the power component for the gripper 410 to perform linear motion relative to the input member 110 along the second direction is arranged on the consumable mechanism 200, it is possible to avoid setting the power component for driving the gripper 410 to perform linear motion relative to the input member 110 along the second direction on the transfer mechanism 400, thereby reducing the weight of the transfer mechanism 400, thereby reducing the vibration and deflection generated by the gripper 410 during the motion, and improving the motion accuracy of the gripper 410. Through the motion of the gripper 410, the reactor 20 can be transferred between different mechanisms.

[0047] See 1. Figure 2 , Figure 3 and Figure 4In some embodiments, the incubation mechanism 500 is also used to carry the reactor 20. When the sampling member 310 adds the sample and the reagent to the reactor 20, the liquid formed by the mixture of the sample and the reagent can be recorded as the reaction liquid. For the reactor 20 containing the reaction liquid, the incubation mechanism 500 can heat the reaction liquid in the reactor 20 at a constant temperature, so that the reaction liquid produces a sufficient chemical reaction under constant temperature conditions. In fact, there are solid magnetic beads in the reagent, so the reaction liquid is actually a suspension. After the sample and the reagent produce a sufficient chemical reaction, the useful substance (substance to be analyzed) will combine with the magnetic beads to form a conjugate, which can be used as a measurement object in the subsequent test and analysis process, so that the detection of different test items of the sample can be achieved.

[0048] The incubation mechanism 500 may carry a plurality of reactors 20, and the incubation mechanism 500 may also perform linear motion along the second direction. The incubation mechanism 500 may be driven by a separate power assembly, or the incubation mechanism 500 may be directly fixed on the sliding member 230, so that the sliding member 230 may simultaneously drive the incubation mechanism 500, the input member 110, the sample tube 210, and the reagent box 220 to perform linear motion along the second direction. By performing linear motion along the second direction of the incubation mechanism 500, the gripper 410 may perform linear motion with three degrees of freedom relative to the incubation mechanism 500, so as to ensure that the gripper 410 can grasp the reactors 20 at different positions in the incubation mechanism 500. In other embodiments, the incubation mechanism 500 may also be fixed on the chassis 830, so that the gripper 410 performs linear motion with two degrees of freedom relative to the incubation mechanism 500. The incubation mechanism 500 and the input member 110 may be spaced apart along the first direction, so as to reduce interference and ensure that the gripper 410 can smoothly grasp the reactor 20 on the incubation mechanism 500 and the input member 110 .

[0049] See also Figure 2 , Figure 3 and Figure 4 In some embodiments, the magnetic collection mechanism 600 has a liquid transfer station 610 and a liquid injection station 620, and the liquid transfer station 610 and the liquid injection station 620 are arranged at intervals along the second direction. The magnetic collection mechanism 600 includes a magnetic collection component 640 and a belt transmission component 650. The magnetic collection component 640 is used to carry the reactor 20. The belt transmission component 650 can drive the magnetic collection component 640 to perform linear motion through a synchronous belt, so that the magnetic collection component 640 carries the reactor 20 to perform reciprocating linear motion between the liquid transfer station 610 and the liquid injection station 620 along the second direction. When the reactor 20 is located at the liquid transfer station 610, the reaction liquid can be injected into the reactor 20.

[0050] See also Figure 2 , Figure 3 and Figure 4In some embodiments, the magnetic collection mechanism 600 further has a transfer station 630. The pipetting station 610, the injection station 620 and the transfer station 630 are arranged at intervals along the movement direction of the magnetic collection assembly 640, that is, the pipetting station 610, the injection station 620 and the transfer station 630 are arranged in a straight line along the second direction, and the injection station 620 can be located between the pipetting station 610 and the transfer station 630, and the reactor 20 can be moved into or out of the entire magnetic collection mechanism 600 at the transfer station 630. In other embodiments, the transfer station 630 can be cancelled, for example, so that the pipetting station 610 and the injection station 620 have the function of the transfer station 630.

[0051] See also Figure 3 , Figure 4 and Figure 5 In some embodiments, the magnetic collection assembly 640 includes a carrier 641 and a magnetic member 642. The carrier 641 is used to carry the reactor 20, and the magnetic member 642 is fixedly arranged on the carrier 641. The carrier 641 has a collection position 643 and a sample loading position 644. Both the collection position 643 and the sample loading position 644 may be provided with a plug hole, and the reactor 20 may be inserted into the plug hole, so as to realize the carrying function of the carrier 641 to the reactor 20. Of course, a clamping body may be arranged at the collection position 643 and the sample loading position 644, and the reactor 20 may be fixed by the clamping body, so that the carrying function of the carrier 641 to the reactor 20 may also be realized. The sample loading position 644 and the collection position 643 are arranged at intervals along the movement direction of the magnetic collection assembly 640, that is, the spacing direction of the sample loading position 644 and the collection position 643 is consistent with the movement direction of the carrier 641.

[0052] See also Figure 3 , Figure 4 and Figure 5 In some embodiments, the number of the sample loading positions 644 is not less than two, and the number of the collection positions 643 is greater than or equal to the number of the sample loading positions 644. For example, the number of the sample loading positions 644 can be two, and the number of the collection positions 643 can be four, etc. By making the number of the sample loading positions 644 and the collection positions 643 multiple, the carrier 641 can simultaneously carry multiple reactors 20 for cleaning and separation operations, thereby improving the working efficiency of the immunoassay analyzer 10. For example, in the case where there are four collection positions 643, the four collection positions 643 are respectively recorded as the first collection position 6431, the second collection position 6432, the third collection position 6433 and the fourth collection position 6434. According to the movement direction of the magnetic collection component 640, the first collection position 6431, the second collection position 6432, the third collection position 6433 and the fourth collection position 6434 are arranged in sequence, the fourth collection position 6434 is closest to the sample loading position 644, and the first collection position 6431 is farthest from the sample loading position 644.

[0053] See also Figure 3 , Figure 4 and Figure 5 , a groove 6411 may be provided on the carrier 641, and the number of the grooves 6411 may be multiple, and the multiple grooves 6411 may be arranged at intervals along the moving direction of the magnetic collection assembly 640, and the groove 6411 may be arranged between two adjacent collection positions 643, and the magnetic member 642 may be fixed in the groove 6411. When there are reactors 20 in two adjacent collection positions 643 located on both sides of the groove 6411, the magnetic member 642 may generate magnetic attraction to the substances in the reactors 20 in the two collection positions 643, so that the two collection positions 643 share one magnetic member 642, avoiding the need to set a magnetic member 642 for each collection position 643, thereby reducing the number of magnetic members 642 used, and further reducing the manufacturing cost of the immunoanalyzer 10. At the same time, by accommodating the magnetic member 642 in the groove 6411, the magnetic member 642 can make full use of the accommodation space of the groove 6411, thereby reducing the occupied space of the entire magnetic collection assembly 640, and further reducing the volume of the immunoanalyzer 10.

[0054] In some embodiments, the groove 6411 on the carrier 641 is arranged on the side of the corresponding collection position 643, and the magnetic member 642 is fixed in the groove 6411. When there is a reactor 20 in the collection position 643 corresponding to the groove 6411, the magnetic member 642 generates a magnetic attraction to the substance in the reactor 20 in the corresponding collection position 643. The magnetic member 642 is arranged on the side of the corresponding collection position 643, which can shorten the length of the magnetic collection assembly 640 along the movement direction, reduce the occupied space of the entire magnetic collection assembly 640, and thus reduce the volume of the immunoassay analyzer 10.

[0055] See also Figure 2 , Figure 3 and Figure 4 In some embodiments, the injection mechanism 700 may include a first injection assembly 710 and a second injection assembly 720. The number of injection stations 620 is two, and the two injection stations 620 are respectively recorded as a first injection station 621 and a second injection station 622. When the magnetic collection assembly 640 moves the reactor 20 to the first injection station 621, the first injection assembly 710 injects the cleaning liquid into the reactor 20 located at the first injection station 621. When the magnetic collection assembly 640 moves the reactor 20 to the second injection station 622, the second injection assembly 720 injects the substrate liquid into the reactor 20 located at the second injection station 622. In other embodiments, the first injection station 621 and the second injection station 622 may be the same station, and the first injection assembly 710 and the second injection assembly 720 may also be the same injection assembly.

[0056] See also Figure 2 , Figure 3 and Figure 4 In some embodiments, the mixing mechanism 810 can mix the liquid in the reactor 20. For example, the mixing mechanism 810 can make the reactor 20 oscillate by eccentric oscillation, thereby mixing the liquid in the reactor 20. The mixing mechanism 810 has at least two mixing positions, and a plug hole can be provided at the mixing position. The reactor 20 can be inserted into the plug hole, so that the mixing position can play a supporting role for the reactor 20. In view of the fact that the number of mixing positions can be multiple, the mixing mechanism 810 can mix the liquids in multiple reactors 20 at the same time, thereby improving the working efficiency of the mixing mechanism 810 and the entire immunoanalyzer 10. The number of mixing mechanisms 810 can be one, so that the number of mixing mechanisms 810 used can be reduced, thereby simplifying the structure of the immunoanalyzer 10, and ultimately reducing the manufacturing cost and volume of the immunoanalyzer 10.

[0057] See also Figure 2 , Figure 3 and Figure 4 In some embodiments, the measuring mechanism 820 is used to measure the reactor 20. In the second direction, the transport mechanism 400 and the sampling mechanism 300 are arranged at intervals along the second direction, the transport mechanism 400 is arranged near one end of the movement track of the magnetic collection component 640, and the sampling mechanism 300 is arranged near the other end of the movement track of the magnetic collection component 640. In the second direction, the measuring mechanism 820 is located between the sampling mechanism 300 and the transport mechanism 400; the input member 110, the sample tube 210 and the reagent kit 220 can also be located between the sampling mechanism 300 and the transport mechanism 400. In this way, each mechanism can be reasonably arranged to improve the utilization rate of the space of the immunoanalyzer 10, and ultimately reduce the occupied space and volume of the immunoanalyzer 10. In the first direction, the consumable mechanism 200, the input mechanism 100 and the incubation mechanism 500 are located on one side of the movement track of the magnetic collection component 640, and the measuring mechanism 820, the injection mechanism 700 and the mixing mechanism 810 are located on the other side of the movement track of the magnetic collection component 640. In this way, the space utilization of the immunoanalyzer 10 can be further improved, and the occupied space and volume of the immunoanalyzer 10 can be ultimately reduced.

[0058] See 2. Figure 3 , Figure 4 and Figure 5 The working principle of the immunoassay analyzer 10 is described below. The working principle can be understood as a method for performing immunoassay on the reactor 20. The working principle can be decomposed into the following working steps:

[0059] In the first step, the gripper 410 makes a two-dimensional linear motion, and the input member 110 makes a linear motion along the second direction. The gripper 410 clamps the empty reactor 20 from the input member 110, and moves the magnetic collection assembly 640, and then moves a sample loading position 644 on the magnetic collection assembly 640 to the transfer station 630. The gripper 410 puts the empty reactor 20 into the sample loading position 644 of the magnetic collection assembly 640 at the transfer station 630, and then the gripper 410 leaves the transfer station 630.

[0060] In the second step, the magnetic collection component 640 drives the empty reactor 20 to move to the pipetting station 610, and the sampling component 310 moves and adds the sample and reagent into the reactor 20 at the pipetting station 610. At this time, the reactor 20 is already filled with the reaction liquid formed by the sample and the reagent.

[0061] For example, the sampling member 310 may first draw a sample from the sample tube 210 and add the sample to the reactor 20, and then move the sampling member 310 to the cleaning tank to clean the inner and outer surfaces of the sampling member 310. After cleaning, the sampling member 310 draws a reagent from the reagent box 220 and adds the reagent to the reactor 20. For another example, the sampling member 310 may first draw a sample from the sample tube 210, and then move the sampling member 310 with the sample drawn to the cleaning tank to clean the outer surface of the sampling member 310, then draw the reagent from the reagent box 220, and then make the sampling member 310 add the sample and the reagent to the reactor 20 at the same time, and then move the sampling member 310 to the cleaning tank to clean the inner and outer surfaces. For another example, the sampling piece 310 can first absorb the reagent from the reagent box 220 and add the reagent to the reactor 20, and then move the sampling piece 310 to the cleaning tank to clean the inner and outer surfaces of the sampling piece 310. After cleaning, the sampling piece 310 absorbs the sample from the sample tube 210 and adds the sample to the reactor 20.

[0062] In the third step, the magnetic collection component 640 drives the reactor 20 containing the reaction liquid to move to the transfer station 630, the gripper 410 moves to the transfer station 630, and moves the reactor 20 from the transfer station 630 to the magnetic collection component 640, and then moves the reactor 20 into the mixing mechanism 810, so that the mixing mechanism 810 mixes the reaction liquid in the reactor 20 so that the reaction liquid can fully react.

[0063] In the fourth step, the gripper 410 moves and removes the reactor 20 after mixing from the mixing mechanism 810, and then moves the reactor 20 into the incubation mechanism 500, so that the incubation mechanism 500 can heat the reaction liquid in the reactor 20 at a constant temperature and react for a certain period of time, so that the useful substances in the reaction liquid can be fully bound to the magnetic beads to form binding substances.

[0064] In the fifth step, taking the magnetic collection component 640 having the first collection position 6431, the second collection position 6432, the third collection position 6433 and the fourth collection position 6434 as an example, the gripper 410 moves and removes the reactor 20 after incubation from the incubation mechanism 500, and the magnetic collection component 640 drives the first collection position 6431 to move to the transfer station 630, and then the gripper 410 places the reactor 20 from the transfer station 630 into the first collection position 6431.

[0065] In the sixth step, the magnetic collection component 640 drives the reactor 20 at the first collection position 6431 to move to the first liquid injection station 621, and the first liquid injection component 710 injects cleaning liquid into the reactor 20 at the first liquid injection station 621, so that the cleaning liquid contacts the binding substance on the magnetic beads, thereby cleaning the binding substance to a certain extent.

[0066] In the seventh step, the magnetic collection component 640 drives the first collection position 6431 to move to the transfer station 630, the gripper 410 moves and moves the reactor 20 out of the first collection position 6431 at the transfer station 630, and then the magnetic collection component 640 drives the second collection position 6432 to move to the transfer station 630, and then the gripper 410 moves and moves the reactor 20 at the transfer station 630 to the second collection position 6432.

[0067] In the eighth step, due to the effect of the magnetic field generated by the magnetic member 642, the magnetic beads will migrate in the reaction solution under the effect of the magnetic member 642 until they are adsorbed onto the inner wall surface of the reactor 20, so that the binding substances bound to the magnetic beads will also be adsorbed onto the inner wall surface of the reactor 20. Generally, all the magnetic beads can be adsorbed onto the inner wall surface of the reactor 20 after 10 to 90 seconds.

[0068] In other embodiments, the cleaning liquid may not be added to the reaction liquid, so that the sixth step may be omitted, so that the seventh step may be directly entered from the fifth step. Therefore, before the reactor 20 containing the reaction liquid is used to absorb the waste liquid, the reactor 20 may be moved to the liquid injection station 620 to inject the cleaning liquid, or the cleaning liquid may not be injected into the reactor 20.

[0069] In the ninth step, the magnetic collection component 640 drives the second collection position 6432 to move to the pipetting station 610, and the sampling piece 310 moves and absorbs the waste liquid in the reactor 20. Then the sampling piece 310 moves to the cleaning tank and discharges the waste liquid into the cleaning tank. The cleaning tank will also clean the inner and outer surfaces of the sampling piece 310 to prevent the residual waste liquid in the sampling piece 310 from causing cross contamination.

[0070] In the tenth step, the magnetic collection component 640 drives the second collection position 6432 to move to the first liquid injection station 621 , and the first liquid injection component 710 injects the cleaning liquid into the reactor 20 located at the first liquid injection station 621 .

[0071] In the eleventh step, the magnetic collection component 640 drives the second collection position 6432 to move to the transfer station 630, the gripper 410 moves and moves the reactor 20 from the transfer station 630 to the magnetic collection component 640, and then moves the reactor 20 into the mixing mechanism 810. The mixing mechanism 810 mixes the suspension formed by the cleaning liquid and the magnetic beads. During the mixing process, the cleaning liquid will clean the conjugate bound to the magnetic beads.

[0072] In the twelfth step, the gripper 410 moves and removes the reactor 20 after mixing is completed out of the mixing mechanism 810, so that the magnetic collection component 640 drives the third collection position 6433 to move to the transfer station 630, and the gripper 410 places the reactor 20 from the transfer station 630 to the third collection position 6433.

[0073] In step 13, due to the effect of the magnetic field generated by the magnetic member 642, the magnetic beads will migrate in the reaction solution under the effect of the magnetic member 6422 until they are adsorbed onto the inner wall surface of the reactor 20, so that the binding substances bound to the magnetic beads will also be adsorbed onto the inner wall surface of the reactor 20. Generally, all the magnetic beads can be adsorbed onto the inner wall surface of the reactor 20 after 10 to 90 seconds.

[0074] In the fourteenth step, the magnetic collection component 640 drives the third collection position 6433 to move to the pipetting station 610, and the sampling piece 310 moves and absorbs the waste liquid in the reactor 20. Then the sampling piece 310 moves to the cleaning tank and discharges the waste liquid into the cleaning tank. The cleaning tank will also clean the inner and outer surfaces of the sampling piece 310 to prevent the residual waste liquid in the sampling piece 310310 from causing cross contamination.

[0075] At this time, steps 10 to 14 can be regarded as a first-order cleaning and separation treatment, that is, the steps from the reactor 20 where the reaction liquid has been absorbed to the step of injecting the cleaning liquid to absorbing the waste liquid again can be recorded as a first-order cleaning and separation treatment. In the case where the sixth step exists, steps 6 to 9 can be regarded as pre-cleaning and separation treatment. The entire cleaning and separation process of a reactor 20 may include multiple-stage cleaning and separation treatments. When the cleaning and separation process includes three or more stages of cleaning and separation treatments, steps 10 to 14 can be cycled multiple times. For example, the entire cleaning and separation process includes a second-stage cleaning and separation treatment, and the above-mentioned steps 10 to 14 will become the first-stage cleaning and separation treatment. When the entire cleaning and separation process includes a second-stage cleaning and separation treatment, steps 10 to 14 can be cycled again. Specifically, the working principle of the immunoassay analyzer 1010 can also be decomposed into the following steps:

[0076] In the fifteenth step, the magnetic collection component 640 drives the third collection position 6433 to move to the first liquid injection station 621 , and the first liquid injection component 710 injects the cleaning liquid into the reactor 20 located at the first liquid injection station 621 .

[0077] In the sixteenth step, the magnetic collection component 640 drives the third collection position 6433 to move to the transfer station 630, the gripper 410 moves and moves the reactor 20 from the transfer station 630 to the magnetic collection component 640, and then moves the reactor 20 into the mixing mechanism 810. The mixing mechanism 810 mixes the suspension formed by the cleaning liquid and the magnetic beads. During the mixing process, the cleaning liquid will clean the conjugate bound to the magnetic beads.

[0078] In the seventeenth step, the gripper 410 moves and removes the reactor 20 after mixing is completed out of the mixing mechanism 810, so that the magnetic collection component 640 drives the fourth collection position 6434 to move to the transfer station 630, and the gripper 410 places the reactor 20 from the transfer station 630 to the fourth collection position 6434.

[0079] In step 18, due to the effect of the magnetic field generated by the magnetic member 642, the magnetic beads will migrate in the reaction solution under the effect of the magnetic member 642 until they are adsorbed onto the inner wall surface of the reactor 20, so that the binding substances bound to the magnetic beads will also be adsorbed onto the inner wall surface of the reactor 20. Generally, all the magnetic beads can be adsorbed onto the inner wall surface of the reactor 20 after 10 to 90 seconds.

[0080] In the nineteenth step, the magnetic collection component 640 drives the fourth collection position 6434 to move to the pipetting station 610, the sampling piece 310 moves and absorbs the waste liquid in the reactor 20, and then the sampling piece 310 moves to the cleaning tank and discharges the waste liquid into the cleaning tank. The cleaning tank will also clean the inner and outer surfaces of the sampling piece 310 to prevent the residual waste liquid in the sampling piece 310 from causing cross contamination.

[0081] After the completion of step 19, that is, after the reactor 20 has finished absorbing the waste liquid for the last time, the effective cleaning and separation process of the entire magnetic beads and the binding substance has actually been completed. At this time, the above steps 15 to 19 will become the second stage cleaning and separation process.

[0082] In the twentieth step, the magnetic collection component 640 drives the fourth collection position 6434 to move to the second liquid injection station 622 , and the second liquid injection component 720 injects the substrate liquid into the reactor 20 located at the second liquid injection station 622 .

[0083] In the twenty-first step, the magnetic collection component 640 drives the fourth collection position 6434 to move to the transfer station 630, the gripper 410 moves and moves the reactor 20 from the transfer station 630 out of the magnetic collection component 640, and then moves the reactor 20 into the mixing mechanism 810, and the mixing mechanism 810 mixes the suspension formed by the substrate liquid, magnetic beads and the conjugate.

[0084] In step 22, the gripper 410 moves and removes the reactor 20 after mixing from the mixing mechanism 810, and then moves the reactor 20 into the incubation mechanism 500, so that the incubation mechanism 500 heats the liquid in the reactor 20 at a constant temperature and reacts for a certain period of time.

[0085] In the twenty-third step, the gripper 410 moves and removes the reactor 20 after incubation from the incubation mechanism 500, and moves the reactor 20 into the measuring mechanism 820 for measurement.

[0086] Step 24: After the measurement of the reactor 20 is completed, the gripper 410 moves and discards the reactor 20 after the measurement is completed.

[0087] In other embodiments, the gripper 410 can directly move the reactor 20 injected with the substrate liquid to the measuring mechanism 820 for measurement after the mixing is completed, so that step 23 can be omitted, so that the process directly proceeds from step 22 to step 24. Therefore, before measuring the reactor 20 containing the substrate liquid, the reactor 20 can be transferred to the incubation mechanism 500 for incubation according to the actual needs, or the reactor 20 can not be transferred to the incubation mechanism 500 for incubation.

[0088] If different pipette needles are used for sample injection, reagent injection, and waste liquid aspiration, on the one hand, the number of pipette needles used will increase, and the number of driving sources used to drive the pipette needles to move will also increase, thereby increasing the manufacturing cost and volume of the immunoassay analyzer 10. On the other hand, in view of the movement of multiple pipette needles, in order to avoid reducing the interference caused by the pipette needles during the movement, the difficulty of controlling the movement of the pipette needles will increase.

[0089] As for the immunoassay analyzer 10 in the above embodiment, since the injection of the sample, the injection of the reagent and the absorption of the waste liquid are all operated by the same sampling member 310, the number of sampling members 310 and the number of driving sources for driving the sampling members 310 to move will be reduced, thereby reducing the manufacturing cost and volume of the immunoassay analyzer 10. At the same time, the difficulty of controlling the movement of a single sampling member 310 is also reduced.

[0090] If the magnetic collection assembly 640 adopts a turntable mode, the turntable drives the reactor 20 to move to different positions to complete the cleaning and separation by rotating, which will also increase the volume of the turntable, and will also increase the number of liquid aspiration needles and the driving source matched with the liquid aspiration needles, and will also increase the manufacturing cost and volume of the immunoassay analyzer 10. At the same time, the movement control difficulty of the turntable with a large weight and volume increases, which is easy to affect the movement accuracy of the turntable and increase the failure rate of the turntable movement.

[0091] As for the immunoassay analyzer 10 in the above embodiment, since the magnetic collection assembly 640 is moved linearly between the transfer station 630, the injection station 620 and the pipetting station 610, the control difficulty of the linear motion will be significantly reduced, so that the accuracy of the movement of the magnetic collection assembly 640 can be improved and the failure rate can be reduced, and the volume of the magnetic collection assembly 640 can be reduced, thereby realizing the miniaturization design of the immunoassay analyzer 10. At the same time, the arrangement direction of the sample adding position 644 and the collection position 643 on the magnetic collection assembly 640 is the same as the movement direction of the magnetic collection assembly 640, effectively avoiding the situation that the arrangement direction of the sample adding position 644 and the collection position 643 on the magnetic collection assembly 640 is perpendicular to the movement direction of the magnetic collection assembly 640, so that on the one hand, the magnetic collection assembly 640 is narrow and long, reducing the volume of the magnetic collection assembly 640 and the entire immunoassay analyzer 10, and on the other hand, the space covered by the magnetic collection assembly 640 during the movement is reduced, further reducing the volume of the immunoassay analyzer 10, and realizing the miniaturization design of the immunoassay analyzer 10.

[0092] If the mixing of the reaction solution formed by the reagent and the sample, the mixing of the cleaning solution and the magnetic beads, and the mixing of the substrate solution and the magnetic beads all adopt different mixing mechanisms 810 modes, the number of mixing mechanisms 810 used will increase, thereby increasing the manufacturing cost and volume of the immunoassay analyzer 10.

[0093] As for the immunoassay analyzer 10 in the above embodiment, since the mixing of the reaction solution, the mixing of the cleaning solution and the magnetic beads, and the mixing of the substrate solution and the magnetic beads all use the same mixing mechanism 810, the number of mixing mechanisms 810 used will be greatly reduced, thereby reducing the manufacturing cost of the immunoassay analyzer 10 and realizing a miniaturized design of the immunoassay analyzer 10.

[0094] Since the immunoassay analyzer 10 has low manufacturing cost, small size and low motion control difficulty, the failure rate of the immunoassay analyzer 10 can be reduced, and the requirements for professional operation of the immunoassay analyzer 10 can be lowered, so that the immunoassay analyzer 10 can be widely used in small and medium-sized laboratories or medical institutions.

[0095] In view of the fact that there are multiple collection positions 643, for two adjacent collection positions 643, the reactor 20 is injected with cleaning liquid at one of the collection positions 643 and then transferred to the other collection position 643 to absorb waste liquid. In this way, the magnetic collection component 640 can carry multiple reactors 20 for cleaning and separation at the same time, that is, the reactors 20 after cleaning and separation can be moved out of the magnetic collection component 640 one by one, and the reactors 20 that need to be cleaned and separated can be moved into the magnetic collection component 640 one by one, thus improving the working efficiency of the immunoassay analyzer 10 to a certain extent.

[0096] Since the sampling member 310 can perform two-dimensional linear motion, and the sample tube 210 and the reagent box 220 can perform linear motion along the second direction, the sampling member 310 can perform three-dimensional linear motion relative to the sample tube 210 and the reagent box 220, ensuring that the sampling member 310 can smoothly absorb the sample and reagent from the sample tube 210 and the reagent box 220, respectively, and at the same time reduce the weight of the sampling mechanism 300 to avoid vibration and deflection of the sampling member 310 during the movement, thereby improving the movement accuracy of the sampling member 310. Moreover, the gripper 410 can perform two-dimensional linear motion, and the input member 110 can perform linear motion along the second direction, so that the gripper 410 can perform three-dimensional linear motion relative to the input member 110, ensuring that the gripper 410 can smoothly grab the reactor 20 from the input member 110, and at the same time reduce the weight of the transport mechanism 400 to avoid vibration and deflection of the gripper 410 during the movement, thereby improving the movement accuracy of the gripper 410. Furthermore, the magnetic collecting assembly 640 carries the reactor 20 to perform linear motion along the second direction. Therefore, this arrangement can simplify the structure of the immunoanalyzer 10 , thereby reducing the manufacturing cost and volume of the immunoanalyzer 10 .

[0097] Referring to 6, by operating the above-mentioned immunoanalyzer 10, the following immunoanalyzer method can be formed, which can be understood as the immunoanalyzer method being performed by the immunoanalyzer 10. The sampling member 310 and the gripper 410 make linear motions along the first direction and the third direction, the carrying assembly for holding samples and reagents and the input member 110 for carrying the reactor 20 make linear motions along the second direction, and the magnetic collection assembly 640 carries the reactor 20 to make linear motions between the pipetting station 610 and the injection station 620 spaced apart along the second direction. On this basis, the immunoanalyzer method can mainly include the following steps:

[0098] S910, the empty reactor 20 on the input part 110 is transferred to the magnetic collection component 640 through the gripper 410, the magnetic collection component 640 carries the empty reactor 20 to the pipetting station 610, the reaction liquid is sucked in the carrying component through the sampling part 310 and injected into the reactor 20, and the reactor 20 is moved out of the magnetic collection component 640 through the gripper 410 to mix the liquid in the reactor 20.

[0099] S920, incubating the mixed reactor 20.

[0100] S930, the reactor 20 after incubation is moved into the magnetic collection assembly 640 through the gripper 410 and moved to the pipetting station 610, the magnetic beads in the reactor 20 are collected on the inner wall of the reactor 20, and the waste liquid is sucked through the sampling piece 310.

[0101] S940, move the magnetic collection component 640 carrying the reactor 20 after absorbing the waste liquid to the injection station 620, inject the cleaning liquid into the reactor 20 through the first injection component 710, and move the reactor 20 out of the magnetic collection component 640 through the gripper 410 to mix the liquid in the reactor 20.

[0102] S950, the reactor 20 containing the cleaning liquid is moved into the magnetic collection assembly 640 through the gripper 410 and moved to the liquid transfer station 610, and the waste liquid is sucked by the sampling piece 310.

[0103] S960 , moving the magnetic collection assembly 640 carrying the reactor 20 to the liquid injection station 620 , and injecting the substrate liquid into the reactor 20 through the second liquid injection assembly 720 .

[0104] S970 , the reactor 20 containing the substrate liquid is moved out of the magnetic collection assembly 640 by the gripper 410 to mix the liquid in the reactor 20 .

[0105] S980, the reactor 20 containing the substrate liquid is transferred to the measuring mechanism 820 for measurement by the gripper 410.

[0106] For other steps of the immunoassay method, reference may be made to the above description of the structure and working principle of the immunoassay analyzer 10 , which will not be described in detail here.

[0107] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0108] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be construed as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent application shall be subject to the attached claims.

Claims

1. An immunoassay method, characterized in that: The first direction, the second direction and the third direction are arranged at an angle to each other, the sampling member and the gripper make linear motion along the first direction and the third direction, the carrying assembly for holding the sample and the reagent and the input member for carrying the reactor make linear motion along the second direction, and the magnetic collection assembly carries the reactor to make linear motion between the pipetting station and the injection station arranged at intervals along the second direction; the immunoassay method comprises the following steps: The empty reactor on the input member is transferred to the magnetic collection assembly by a gripper, the magnetic collection assembly carries the empty reactor and moves to the liquid transfer station, the reaction liquid is sucked from the carrying assembly by the sampling member and injected into the reactor, and the reactor is moved out of the magnetic collection assembly by the gripper to mix the liquid in the reactor; Incubate the mixed reactor; The incubated reactor is moved into the magnetic collection assembly and moved to the liquid transfer station by the gripper, the magnetic beads in the reactor are collected on the inner wall of the reactor, and the waste liquid is sucked by the sampling piece; The magnetic collection assembly carries the reactor after absorbing the waste liquid and moves it to the liquid injection station, the cleaning liquid is injected into the reactor through the first liquid injection assembly, and the reactor is moved out of the magnetic collection assembly by a gripper to mix the liquid in the reactor; The reactor containing the cleaning liquid is moved into the magnetic collection assembly by the gripper and moved to the liquid transfer station, and the waste liquid is sucked by the sampling member; The magnetic collection assembly carrying the reactor is moved to the liquid injection station, and the substrate liquid is injected into the reactor through the second liquid injection assembly; Using a gripper, the reactor containing the substrate liquid is moved out of the magnetic collection assembly to mix the liquid in the reactor; and The reactor containing the substrate liquid is transferred to the measuring mechanism for measurement by the gripper; The magnetic collection assembly has a sample loading position and a collection position for carrying the reactor, the sample loading position and the collection position are arranged at intervals along the moving direction of the magnetic collection assembly, the sample loading position can be moved to the liquid transfer station, and the collection position can be moved to the liquid transfer station or the liquid injection station; There are multiple collection positions. For two adjacent collection positions, the reactor is injected with cleaning liquid at one of the collection positions and then transferred to the other collection position to absorb waste liquid.

2. The immunoassay method according to claim 1, characterized in that: The reactor is carried by an incubation mechanism capable of linear motion along the first direction for incubation.

3. The immunoassay method according to claim 1, characterized in that: The liquid in the reactor is mixed by the same mixing mechanism.

4. The immunoassay method according to claim 1, characterized in that: After the sampling component is injected with reaction liquid or absorbs waste liquid, the sampling component is cleaned.

5. The immunoassay method according to claim 1, characterized in that: The step of injecting cleaning liquid into the reactor into which the reaction liquid has been completely absorbed and then absorbing the waste liquid is recorded as a first-stage cleaning and separation process. The immunoassay method includes multiple stages of the cleaning and separation process.

6. The immunoassay method according to claim 1, characterized in that: The number of the injection stations is two, and the two injection stations are respectively recorded as the first injection station and the second injection station. The cleaning liquid is injected into the reactor at the first injection station; after the reactor absorbs the waste liquid for the last time, the substrate liquid is injected into the reactor at the second injection station.

7. The immunoassay method according to claim 1, characterized in that: Before the reactor containing the reaction liquid is used to absorb the waste liquid, the reactor is moved to the liquid injection station to inject the cleaning liquid.

8. The immunoassay method according to claim 1, characterized in that: Between the injection of substrate solution into the reactor and the measurement, the reactor is moved out of the magnetic collection assembly by a gripper for incubation.

9. The immunoassay method according to claim 1, characterized in that: The number of the sample loading positions is no less than two.

10. The immunoassay method according to claim 1, characterized in that: The number of the collection positions is greater than or equal to the number of the sample loading positions.

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