Biological reaction system

Abstract

The present application discloses a biological reaction system, which includes a microfluidic chip, a fixture, and an infusion device. The fixture includes a fixture body and a fixing component. A receiving groove is formed on the first surface of the fixture body. The second surface of the fixture body is opposite to the first surface. The receiving groove is used for placing the microfluidic chip. The fixture body is further provided with a liquid injection flow channel and a liquid extraction flow channel. The first end of the liquid injection flow channel is used to convey liquid to the microfluidic chip. The liquid discharged from the microfluidic chip flows into the receiving groove, and the receiving groove is communicated with the first end of the liquid extraction flow channel. The fixing component is arranged on the fixture body and is used to fix the microfluidic chip in the receiving groove. The infusion device is respectively connected to the second end of the liquid injection flow channel and the second end of the liquid extraction flow channel, and is used to perform liquid replacement on the microfluidic chip.

Description

Technical Field

[0001] This application relates to the field of bioculture technology, and particularly to a bioreaction system. Background Art

[0002] Currently, bioreactions are carried out through the culture environment of a bioreaction system. For example, organ cell culture, tumor cell culture, and cell drug tolerance experiments. Generally, a bioreaction system includes: a microfluidic chip, a fixture, and an infusion device. A capillary and a cavity are provided in the microfluidic chip. The cavity is used for bioreaction, and the capillary is used to transport the liquid required for bioreaction to the cavity. The microfluidic chip is fixed by the fixture. The fixture is configured with a flow channel that connects the capillary of the microfluidic chip and the infusion device. The infusion device replaces the liquid in the capillary through this flow channel, so as to continuously transport the liquid required for bioreaction to the cavity. In the above liquid replacement method, due to the pump pressure of the infusion device, it is difficult for the liquid in the cavity to flow back to the capillary. As a result, during the liquid replacement process, only the liquid in the capillary is replaced, and the waste liquid generated during the bioreaction process is not discharged, making it difficult to create a good environment required for bioreaction. Summary of the Invention

[0003] The main purpose of the embodiments of this application is to propose a bioreaction system for discharging the waste liquid left by the microfluidic chip during the biocell culture process, so as to create a better culture environment for biocells.

[0004] In a first aspect, the embodiments of this application provide a bioreaction system, which includes: a microfluidic chip, a fixture, and an infusion device;

[0005] The fixture includes: a fixture body and a fixing component. A receiving groove is formed on the first surface of the fixture body. The second surface of the fixture body is opposite to the first surface. The receiving groove is used to place the microfluidic chip. The fixture body is also provided with a liquid injection flow channel and a liquid extraction flow channel. The first end of the liquid injection flow channel is used to transport liquid to the microfluidic chip. The liquid discharged from the microfluidic chip flows into the receiving groove, and the receiving groove is communicated with the first end of the liquid extraction flow channel; The fixing component is arranged on the fixture body and is used to fix the microfluidic chip in the receiving groove;

[0006] An infusion device, which is respectively connected to the second end of the liquid injection flow channel and the second end of the liquid extraction flow channel, and is used to replace the liquid of the microfluidic chip.

[0007] An embodiment of the present application provides a biological reaction system, which includes a microfluidic chip, a fixture, and an infusion device. The fixture includes a fixture body and a fixing component. A receiving groove is formed on the first surface of the fixture body. The second surface of the fixture body is opposite to the first surface. The receiving groove is used to place the microfluidic chip. The fixture body is further provided with a liquid injection channel and a liquid extraction channel. The first end of the liquid injection channel is used to deliver liquid to the microfluidic chip. The liquid discharged from the microfluidic chip flows into the receiving groove. The receiving groove is communicated with the first end of the liquid extraction channel. The fixing component is arranged on the fixture body and is used to fix the microfluidic chip in the receiving groove. The infusion device is respectively connected to the second end of the liquid injection channel and the second end of the liquid extraction channel and is used to replace the liquid in the microfluidic chip. In the biological reaction system provided by the embodiment of the present application, the perfusion device delivers liquid to the microfluidic chip through the liquid injection channel of the fixture. The liquid flowing out of the microfluidic chip flows into the receiving groove of the fixture. The liquid in the receiving groove is pumped away by the perfusion device from the liquid extraction channel. Thus, the waste liquid generated and remaining in the microfluidic chip during the biological cell culture process is discharged. Compared with the microfluidic chip that only discharges liquid from its own liquid outlet, the waste liquid discharge rate is higher, and a better culture environment for biological cells can be created. Description of the Drawings

[0008] Figure 1 is a schematic structural diagram of a biological reaction system provided by an embodiment of the present application;

[0009] Figure 2 is a schematic structural diagram of a microfluidic chip provided by an embodiment of the present application;

[0010] Figure 3 is a schematic structural diagram of a fixture for a microfluidic chip provided by an embodiment of the present application;

[0011] Figure 4 is a schematic structural diagram of a fixture for a microfluidic chip provided by an embodiment of the present application;

[0012] Figure 5 is a schematic cross-sectional structural diagram of a fixture provided by an embodiment of the present application;

[0013] Figure 6 is a schematic semi-cross-sectional structural diagram of a fixture body provided by an embodiment of the present application;

[0014] Figure 7 is a schematic structural diagram of a fixture provided by an embodiment of the present application;

[0015] Figure 8 is a schematic semi-cross-sectional structural diagram of a fixture body provided by an embodiment of the present application;

[0016] Figure 9 is a schematic bottom view of a fixture provided by an embodiment of the present application;

[0017] Figure 10 It is a schematic diagram of a fixture provided by an embodiment of the present application;

[0018] Figure 11 It is a schematic diagram of a fixture for a microfluidic chip provided by an embodiment of the present application.

[0019] Figure 12 It is a half-sectional view of a fixture provided by an embodiment of the present application along the center of two guiding holes;

[0020] Figure 13 It is an exploded view of a fixture provided by an embodiment of the present application;

[0021] Figure 14 It is an exploded view of a fixture provided by an embodiment of the present application;

[0022] Figure 15 It is a schematic structural diagram of a perfusion device provided by an embodiment of the present application;

[0023] Figure 16 It is a partial structural schematic diagram of a perfusion device provided by an embodiment of the present application;

[0024] Figure 17 It is an exploded view of a perfusion device provided by an embodiment of the present application;

[0025] Figure 18 It is a schematic structural diagram of a first clamping component provided by an embodiment of the present application;

[0026] Figure 19 It is a schematic structural diagram of a first clamping component provided by an embodiment of the present application;

[0027] Figure 20 It is a schematic structural diagram of a second clamping component provided by an embodiment of the present application;

[0028] Figure 21 It is a schematic structural diagram of a second clamping component provided by an embodiment of the present application;

[0029] Figure 22 It is a schematic structural diagram of a combination of a first clamping component and a second clamping component provided by an embodiment of the present application;

[0030] Figure 23 It is a schematic structural diagram of a first fixing member provided by an embodiment of the present application;

[0031] Figure 24 It is a schematic structural diagram of a second fixing member provided by an embodiment of the present application;

[0032] Figure 25It is a schematic structural diagram of a fixture provided by an embodiment of the present application;

[0033] Figure 26 It is a schematic structural diagram of a biological reaction system provided by an embodiment of the present application;

[0034] Figure 27 It is a schematic structural diagram of a microfluidic chip provided by an embodiment of the present application;

[0035] Figure 28 It is a schematic cross-sectional view of a capillary tube provided by an embodiment of the present application.

[0036] Description of reference numerals:

[0037] 300, Microfluidic chip; 31, Chip body; 311, Liquid inlet; 312, Liquid outlet; 313, Second anti-fooling part; 32, Cavity; 33, Capillary; 331, Micropore; 3311, First opening; 3312, Second opening; 100, Fixture; 11, Fixture body; 111, Accommodating groove; 1111, First recess; 1112, Second recess; 1113, First anti-fooling part; 1114, Liquid outlet groove; 112, Connecting groove; 113, Observation through hole; 114, Liquid injection channel; 1141, First liquid injection channel segment; 1142, Second liquid injection channel segment; 115, Liquid extraction channel; 1151, First liquid extraction channel segment; 1152, Second liquid extraction channel segment; 116, Anti-overflow groove; 117, First connection component; 1171, First protruding part; 1172, Second protruding part; 1181, Sliding groove; 1182, Spring hole; 119, Fixed groove; 12, Fixing component; 121, Sliding piece; 122, Spring; 123, Elastic piece; 1231, Fixing piece; 1232, Pushing piece; 13, Fixture cover; 131, Second connection component; 1311, First groove; 1312, Second groove; 132, Guiding hole; 1321, First guiding hole segment; 1322, Second guiding hole segment; 14, Tray; 15, Tray handle; 16, Support plate; M1, First surface of the microfluidic chip; M2, Second surface of the microfluidic chip; M3, Third surface of the microfluidic chip; F1, First surface of the fixture body; F2, Second surface of the fixture body; S1, First surface of the fixture cover; S2, Second surface of the fixture cover; 300, Perfusion device; 31, First perfusion syringe; 311, Accommodating part of the first perfusion syringe 31; 312, Piston part of the first perfusion syringe 31; 32, Second perfusion syringe; 321, Accommodating part of the second perfusion syringe 32; 322, Piston part of the second perfusion syringe 32; 33, Base; 331, Second fixing piece; 3311, Second fixing groove; 3312, Second limiting piece; 332, Main seat; 333, First support; 334, Second support; 335, Clamping component mounting plate; 34, Driving component; 341, Guide rod; 342, Driving lead screw; 35, Slide block component; 351, Slide block part; 352, Connecting piece; 353, Slide base part; 354, First fixing piece; 3541, First fixing groove; 3542, First limiting piece; 355, Fastening piece; 36, First clamping component; 361, First supporting part; 362, First cover plate part; 363, First fixing stud; 364, Handheld part; 365, Hanging hook part; 366, First positioning piece; 367, First clamping plate part; 368, First locking piece; 37, Second clamping component; 371, Second supporting part; 372, Second cover plate part; 373, Second fixing stud; 374, Third fixing stud; 375, Support frame part; 376, Index pin; 377, Second clamping plate part; 378, Second locking piece; 38, Carry-over pin; 39, Perfusion pump;; 400, Biological reaction system; 41, Connecting pipe;42. First storage cabinet; 421. Cable tie; 43. Second storage cabinet; 431. Compartment groove; 432. Carrier plate. Detailed implementation mode

[0038] It should be understood that the specific embodiments described herein are only used to explain this application and are not used to limit this application.

[0039] In the subsequent description, suffixes such as "module", "component" or "unit" used to represent components are only for the convenience of explaining this application, and they have no specific meaning by themselves. Therefore, "module", "component" or "unit" can be used interchangeably.

[0040] An embodiment of this application provides a biological reaction system, which includes: a microfluidic chip, a fixture and an infusion device. The fixture includes: a fixture body and a fixing component. A receiving groove is formed on the first surface of the fixture body. The second surface of the fixture body is opposite to the first surface. The receiving groove is used to place the microfluidic chip. The fixture body is also provided with a liquid injection flow channel and a liquid extraction flow channel. The first end of the liquid injection flow channel is used to deliver liquid to the microfluidic chip. The liquid discharged from the microfluidic chip flows into the receiving groove, and the receiving groove is communicated with the first end of the liquid extraction flow channel. The infusion device is respectively connected to the second end of the liquid injection flow channel and the second end of the liquid extraction flow channel, and is used to replace the liquid in the microfluidic chip.

[0041] Please refer to Figure 1 , Figure 1 shows a schematic structural diagram of a biological reaction system provided by an embodiment of this application. As Figure 1 shown, the biological reaction system 400 includes: a fixture 100 and an infusion device 300. The filling device 300 includes: a first injector 31, a second injector 32 and an infusion pump 39. The infusion pump 39 drives one of the first injector 31 and the second injector 32 to achieve the liquid extraction function, and the other to achieve the liquid injection function. The first injector 31 and the second injector 32 are connected to the fixture 100 through a connecting pipe 41. The fixture 100 includes a fixture body 11. In this embodiment, both connecting pipes 41 include an infusion pipe and a needle. One end of one infusion pipe is connected to an injector, and the other end is communicated with the liquid injection flow channel on the fixture 100 through a needle. One end of the other infusion pipe is connected to the other injector, and the other end is communicated with the liquid extraction flow channel on the fixture 100 through a needle. Exemplarily, the first injector 31 is used for liquid extraction, and the second injector 32 is used for liquid injection.

[0042] Please refer to Figure 2 , Figure 2 shows a schematic structural diagram of a microfluidic chip provided by an embodiment of this application. As Figure 2As shown, the microfluidic chip 300 includes: a chip body 31, a cavity 32, and a capillary 33. The opening of the cavity 32 is located on the second surface M2 of the chip body 31, and the capillary 33 is located in the cavity 32. A liquid inlet 311 and a liquid outlet 312 are further provided on the third surface F3 of the chip body 31, and the capillary 33 can exchange liquid through the liquid inlet 311 and the liquid outlet 312. The second surface M2 of the chip body 31 is opposite to the first surface M1, and the third surface M3 of the chip body 31 is perpendicular to the first surface M1 and the second surface M2 of the chip body 31.

[0043] Please refer to Figure 3 , Figure 3 which shows a schematic structural diagram of a fixture for a microfluidic chip provided by an embodiment of the present application. As Figure 3 shown, the fixture 100 includes: a fixture body 11 and a fixing component 12. The fixing component 12 is used to fix the microfluidic chip 300 in the fixture body 11.

[0044] Exemplarily, the fixture 100 is placed horizontally during use. The first surface F1 of the fixture body 11 is a horizontal plane facing downwards and towards the bottom of the accommodation cavity, that is, the first surface F1 of the fixture body 11 is the bottom surface of the fixture body 11, and the second surface F2 of the fixture body 11 is a horizontal plane facing upwards, that is, the second surface F2 of the fixture body 11 is the top surface. When the microfluidic chip 300 is fixed to the fixture 100, the first surface M1 of the chip body 31 is placed facing downwards, that is, the first surface M1 of the chip body 31 is the bottom surface of the microfluidic chip 300, and when the first surface M1 of the chip body 31 is placed facing upwards, that is, the first surface M1 of the chip body 31 is the top surface. It should be noted that the chip body 31 is the main part of the microfluidic chip 300, and the first surface M1, the second surface M2, and the third surface M3 of the chip body 31 can also be used as the first surface M1, the second surface M2, and the third surface M3 of the microfluidic chip 300.

[0045] Please refer to Figure 2 and Figure 4 , Figure 4 which shows a schematic structural diagram of a fixture for a microfluidic chip provided by an embodiment of the present application. As Figure 4 shown, the fixing component 12 is provided on the fixture body 11 and is used to fix the microfluidic chip 300 in the accommodation groove 111. The accommodation groove 111 is provided on the first surface F1 of the fixture body 11. The accommodation groove 111 is used to place the microfluidic chip 300 as Figure 2 shown. The cavity 32 of the microfluidic chip 300 communicates with the accommodation groove 111, and the liquid flowing out from the opening of the cavity 32 flows into the accommodation groove 111. When the liquid in the cavity 32 of the microfluidic chip 300 overflows from the cavity 32, it flows into the accommodation groove 111 through the communication groove 112.

[0046] Both ends of the receiving groove 111 are respectively used to limit both ends of the microfluidic chip 300, and one end of the receiving groove 111 is also used to install the fixing component 12. When the fixing component 12 is in the extended state, it is convenient for the microfluidic chip 300 to be placed into the receiving groove 111; when the fixing component 12 is in the reset state, it can fix the microfluidic chip 300 placed in the receiving groove 111.

[0047] Please refer to Figure 5 , Figure 5 which shows a schematic cross-sectional structure diagram of a fixture provided by an embodiment of the present application. As Figure 5 shown, the fixture body 11 is provided with a liquid injection flow channel 114. The first end of the liquid injection flow channel 114 is used to connect with the second injector 32 as Figure 1 shown, and the second end of the liquid injection flow channel 114 is used to connect with the liquid inlet 311 of the microfluidic chip 300. The first end of the liquid injection flow channel 114 is arranged on the second surface F2 of the fixture body 11, and the second end of the liquid injection flow channel 114 is opened on the side wall of the receiving groove 111, and this side wall corresponds to the third surface M3 of the microfluidic chip 300. When the microfluidic chip 300 is fixed in the receiving groove 111, the second end of the liquid injection flow channel 114 is connected to the liquid inlet 311 of the microfluidic chip 300. A sealing member is also provided at the second end of the liquid injection flow channel 114. For example, the sealing member is one of a rubber ring or a silica gel ring to ensure the sealing between the second end of the liquid injection flow channel 114 and the liquid inlet 311 of the microfluidic chip 300.

[0048] Please refer to Figure Figure 6 , Figure 6 which shows a schematic semi-cross-sectional structure diagram of a fixture body provided by an embodiment of the present application. As Figure 6 shown, the fixture body 11 is provided with a liquid extraction flow channel 115. The first end of the liquid extraction flow channel 115 is opened on the second surface F2 of the fixture body 11. The second end of the liquid extraction flow channel 115 is communicated with the receiving groove 111 (shown as the area where the receiving groove 111 is located in the figure).

[0049] The first end of the liquid extraction flow channel 115 is arranged on the second surface F2 of the fixture body 11 and is used to connect with the first injector 31 as Figure 1 shown. The second end of the liquid extraction flow channel 115 is connected to the receiving groove 111 and is used to extract the liquid in the receiving groove 111 through the first injector 31 when the liquid level height in the receiving groove 111 reaches a preset height, so as to reduce the liquid level height in the receiving groove 111.

[0050] In the biological reaction system provided by the embodiments of the present application, the perfusion device conveys liquid to the microfluidic chip through the liquid injection flow channel of the clamp. The liquid flowing out of the microfluidic chip flows into the accommodating groove of the clamp, and the liquid in the accommodating groove is pumped away by the perfusion device from the liquid extraction flow channel. Thus, the waste liquid generated and remaining in the microfluidic chip during the biological cell culture process is discharged. Compared with the microfluidic chip that only discharges liquid from its own liquid outlet, the waste liquid discharge rate is higher, and a better culture environment for biological cells can be created.

[0051] To more clearly introduce the technical solution of the present application, the technical solution of the present application will also be introduced through specific embodiments below. It should be noted that this specific embodiment is used to expand and explain the technical solution of the present application, rather than limiting the present application.

[0052] Please refer to Figure 7 , Figure 7 which shows a schematic structural diagram of a clamp provided by the embodiments of the present application. As Figure 7 shown, the clamp 100 further includes a tray 14. The tray 14 is used to place the clamp body 11. The clamp 11 is placed in the accommodating cavity provided by the tray 14. Among them, multiple clamp bodies 11 can be placed in one tray 14. The tray 14 is used to provide a separate accommodating cavity for each clamp body 11. The clamp body 11 is used to place the microfluidic chip 300. After the liquid in the microfluidic chip 300 flows out, it flows into the accommodating cavity provided by the tray 14. Since the first surface F1 of the clamp body 11 faces the bottom of the accommodating cavity, that is, the opening of the accommodating groove 111 faces the accommodating cavity, the liquid gathers in the accommodating cavity, which is equivalent to gathering in the accommodating groove 111. After gathering to a certain height, it is pumped away by the first perfusion device 31 as Figure 1 shown.

[0053] In some embodiments, as Figure 4 shown, a communication groove 112 is further formed on the first surface F1 of the clamp body 11. The communication groove 112 is connected to the accommodating groove 111, and the depth of the communication groove 112 is greater than the depth of the accommodating groove 111. It should be noted that in the embodiments of the present application, the reference plane of the depth is the first surface F1 of the clamp body 11.

[0054] As Figure 5 shown, the liquid in the microfluidic chip 300 overflows from the opening of the cavity 32 and is discharged through the communication groove 112.

[0055] In the embodiments of the present application, the accommodating groove 111 and the cavity 32 of the microfluidic chip 300 are communicated through the communication groove 112 to ensure that after the liquid overflows from the cavity 32, it can quickly flow into the accommodating groove 111.

[0056] In some embodiments, as Figure 4As shown, an observation through-hole 113 is further provided between the first surface F1 and the second surface F2 of the fixture body 11, and the observation through-hole 113 communicates with the accommodation groove 111.

[0057] The observation through-hole 113 is used to provide light illumination to the microfluidic chip 300 from the second surface F2 of the fixture body 11, and the observation through-hole 113 also facilitates the user to take out the biological sample in the microfluidic chip 300 from the microfluidic chip 300.

[0058] In some embodiments, as Figure 4 shown, a liquid outlet groove 1114 is further provided on the first surface F1 of the fixture body 11. The liquid outlet groove 1114 communicates with the accommodation groove 111 at the side wall of the accommodation groove 111, and this side wall corresponds to the third surface M3 of the microfluidic chip 300. As Figure 2 shown, when the microfluidic chip 300 as shown is placed in the accommodation groove 111, the liquid outlet 312 of the microfluidic chip 300 is located in the accommodation groove 111, and the liquid outlet 312 of the microfluidic chip 300 corresponds to the liquid outlet groove 1114. The liquid flowing out from the liquid outlet 312 can be discharged into the accommodation groove 111 more quickly through the liquid outlet groove 1114.

[0059] In some embodiments, as Figure 4 shown, a spill prevention groove 116 is further provided on the first surface F1 of the fixture body 11. The spill prevention groove 116 communicates with the accommodation groove 111.

[0060] Please refer to Figure 8 , Figure 8 which shows a schematic semi-sectional structure diagram of a fixture body provided by an embodiment of the present application. As Figure 8 shown, the area within the dashed box in the figure shows the accommodation groove 111, the spill prevention groove 116, and the observation through-hole 113. The spill prevention groove 116 and the accommodation groove 111 are in a stepped shape. The depth of the spill prevention groove 116 is greater than the depth of the accommodation groove 111, and the reference plane for the depth is the first surface F1 of the fixture body 11. The depth difference between the spill prevention groove 116 and the accommodation groove 111 is: 0.5 mm - 3 mm. For example, the depth difference between the spill prevention groove 116 and the accommodation groove 111 is 1.7 mm.

[0061] Due to the capillary phenomenon of the liquid, the liquid in the accommodation groove 111 converges along the side wall of the accommodation groove 111 towards the second end of the liquid extraction channel 115. When the liquid level height in the accommodation groove 111 has not reached the preset height, the second end of the liquid extraction channel 115 can also extract the liquid from the accommodation groove 111. This abnormal liquid extraction phenomenon results in an insufficient liquid level height in the accommodation groove 111, thereby leading to an insufficient liquid storage in the microfluidic chip 300, and further leading to the failure to guarantee the biological reaction conditions in the microfluidic chip 300.

[0062] In the embodiments of the present application, by providing an anti-overflow groove 116 at the second end of the liquid extraction channel 115, and there is a height difference between the anti-overflow groove 116 and the accommodation groove 111. Therefore, when the liquid in the accommodation groove 111 reaches the anti-overflow groove 116 due to capillary action, liquid beads are first generated on the side wall of the anti-overflow groove 116. Under the action of gravity, most of the liquid beads fall off, thereby reducing the number of liquid beads reaching the second end of the liquid extraction channel 115, and further avoiding excessive extraction of the liquid in the accommodation groove 111, and improving the balance degree of the liquid replacement process.

[0063] In addition, it is also necessary to ensure that the distance between the second end of the liquid extraction channel 115 and the side wall of the anti-overflow groove 116 is: 2 mm - 5 mm, so as to obtain a better anti-capillary effect.

[0064] Please refer to Figure 9 , Figure 9 which shows a schematic bottom view of a fixture provided by the embodiments of the present application. As Figure 9 shown, the side wall of the anti-overflow groove 116 includes an arc-shaped side wall section with the second end of the liquid extraction channel 115 as the center of the circle and a radius of r. The value range of r is: 2 mm - 5 mm. For example, r can be any one of 2.4 mm, 3.5 mm or 4.5 mm. By defining the distance between the second end of the liquid extraction channel 115 and the side wall of the anti-overflow groove 116, the liquid beads generated by capillary action can drip before converging to the second end of the liquid extraction channel 115, thereby reducing the number of liquid beads reaching the second end of the liquid extraction channel 115, and further avoiding excessive extraction of the liquid in the accommodation groove 111.

[0065] In some embodiments, as Figure 5 shown, the first end to the second end of the liquid injection channel 114 at least includes a first liquid injection channel section 1141 and a second liquid injection channel section 1142. The diameter of the first liquid injection channel section 1141 is greater than the diameter of the second liquid injection channel section 1142, and the connection between the first liquid injection channel section 1141 and the second liquid injection channel section 1142 is in an inclined shape.

[0066] In some embodiments, as Figure 6 shown, the first end to the second end of the liquid extraction channel 115 at least includes a first liquid extraction channel section 1151 and a second liquid extraction channel section 1152. The diameter of the first liquid extraction channel section 1151 is greater than the diameter of the second liquid extraction channel section 1152, and the connection between the first liquid extraction channel section 1151 and the second liquid extraction channel section 1152 is in an inclined shape.

[0067] Generally, the diameters of the liquid injection channel 114 and the liquid extraction channel 115 are the same as the diameter of the perfusion part. However, such a setting is not conducive to the insertion of the perfusion part, which not only reduces the operation efficiency but also may bend the needle during the insertion of the perfusion part. Therefore, in the embodiments of the present application, the first ends of the liquid injection channel 114 and the liquid extraction channel 115 are used for inserting the perfusion part. The diameter of the first liquid injection channel section 1141 at the first end of the liquid injection channel 114 is larger than the diameter of the second liquid injection channel section 1142, and the diameter of the first liquid extraction channel section 1151 at the first end of the liquid extraction channel 115 is larger than the diameter of the second liquid extraction channel section 1152, so as to facilitate the insertion of the perfusion part from the first end of the liquid injection channel 114. At the same time, in the embodiments of the present application, the joints between the first liquid injection channel section 1141 and the second liquid injection channel section 1142, and between the first liquid extraction channel section 1151 and the second liquid extraction channel section 1152 are processed into an inclined surface shape, so that the joint achieves a transition from large to small, improving the passing performance of the perfusion part at the joint, thereby improving the operation efficiency of the user.

[0068] In some embodiments, the inclined surface shape includes a conical shape and also includes other shapes that gradually decrease from large to small, for example, an arc shape. It should be noted that the inclined surface shape provided in the embodiments of the present application is only used for a specific introduction of the embodiments and does not limit the present application.

[0069] It should be noted that in the above embodiments, a sealing member is also provided at the first liquid injection channel section 1141 and the first liquid extraction channel section 1151. For example, the sealing member is one of a rubber ring or a silica gel ring to ensure the sealing performance between the first liquid injection channel section 1141, the first liquid extraction channel section 1151 and the perfusion part.

[0070] In some embodiments, please refer to Figure 10 , Figure 10 which shows a schematic diagram of a fixture provided in the embodiments of the present application. As Figure 10 shown, the accommodating groove 111 includes a first recessed portion 1111 and a second recessed portion 1112 opened along the side direction of the microfluidic chip 300. The first recessed portion 1111 is communicated with the anti-overflow groove 116. The cross-sectional shape of the microfluidic chip 300 on the first surface M1 is a rectangle, and the long side of the rectangle is the side of the microfluidic chip 300. In this way, both the liquid capacity in the accommodating groove 111 is enlarged and it is convenient for the user to take and place the microfluidic chip 300 in the accommodating groove 111.

[0071] In some embodiments, as Figure 10As shown, one end of the accommodation groove 111 is further provided with a first anti-fooling portion 1113. Specifically, the first anti-fooling portion 1113 is disposed at two corners at one end of the accommodation groove 111, one is in the shape of a groove and the other is in the shape of an arc. The microfluidic chip 300 includes a second anti-fooling portion 213. Specifically, the second anti-fooling portion 213 is disposed at two corners at one end of the microfluidic chip 300, one is a right angle and the other is subjected to an inscribing process. When the first anti-fooling portion 1113 and the second anti-fooling portion 213 cooperate, the corner of the microfluidic chip 300 subjected to the inscribing process can only be placed at the corner of the accommodation groove 111 in the shape of an arc, and the right-angle corner of the microfluidic chip 300 can only be placed at the accommodation groove 111 in the shape of a groove, thereby preventing the microfluidic chip 300 from being placed in the reverse direction.

[0072] In some embodiments, please refer to Figure 11 , Figure 11 which shows a schematic diagram of a fixture for a microfluidic chip provided by an embodiment of the present application. As Figure 11 shown, a first connection component 117 is provided on the second surface F2 of the fixture body 11. The fixture 100 further includes a fixture cover 13. The fixture cover 13 includes: a second connection component 131 and two guiding holes 132. The second connection component 131 is used to connect with the first connection component 117 of the fixture body 11, and the fixture body 11 and the fixture cover 13 can be connected through the first connection component 117 and the second connection component 131 to form an integral body. The two guiding holes 132 communicate the second surface S2 and the first surface S1 of the fixture cover 13, and the second surface S2 and the first surface S1 of the fixture cover 13 are opposite to each other.

[0073] The two guiding holes 132 respectively correspond to the first end of the liquid injection flow channel 114 and the first end of the liquid extraction flow channel 115.

[0074] Please refer to Figure 12 , Figure 12 which shows a half-sectional view of a fixture provided by an embodiment of the present application along the centers of the two guiding holes. As Figure 12 shown, the port of the guiding hole 132 on the second surface S2 of the fixture cover 13 is the first end, and the port of the guiding hole 132 on the first surface S1 of the fixture cover 13 is the second end. The first end of the guiding hole 132 is used to insert a perfusion member. After the fixture body 11 and the fixture cover 13 form an integral body, the second ends of the two guiding holes 132 respectively correspond to the first end of the liquid injection flow channel 114 and the first end of the liquid extraction flow channel 115.

[0075] The fixture cover 13 is used to reduce the number of impurities falling into the microfluidic chip 300 from the observation through hole 113. The guiding holes 132 on the fixture cover 13 can also assist the user to insert the perfusion member into the liquid injection flow channel 114 and the liquid extraction flow channel 115, so as to improve the operation efficiency of the user.

[0076] In some embodiments, asFigure 11 As shown, the first connection component 117 includes: a first protrusion 1171 and a second protrusion 1172, and the second connection component 131 includes: a first groove 1311 and a second groove 1312. The first protrusion 1171 and the second protrusion 1172 are respectively used to connect with the first groove 1311 and the second groove 1312.

[0077] Exemplarily, the sizes or shapes of the first groove 1311 and the second groove 1312 are different. The size and shape of the first groove 1311 are the same as those of the first protrusion 1171, and the size and shape of the second groove 1312 are the same as those of the second protrusion 1172. In this way, it is ensured that the second protrusion 1172 and the first protrusion 1171 cannot be reversely placed in the first groove 1311 and the second groove 1312 at the same time, thereby preventing the reverse mating of the fixture body 11 and the fixture cover 13.

[0078] It should be noted that the first connection component 117 can also be arranged on the fixture cover 13, and the second connection component 131 can be arranged on the fixture body 11. The number and shape of the components that form a fit in the first connection component 117 and the second connection component 131 are not fixed. The embodiments of the present application are only for specific illustration and do not limit the present application.

[0079] In some embodiments, the first connection component 117 and the second connection component 131 cooperate to prevent the reverse connection of the fixture cover 13 and the fixture body 11.

[0080] Exemplarily, the first connection component 117 and the second connection component 131 can only form a fit at one angle. Specifically, the first connection component 117 includes: a first protrusion 1171 and a second protrusion 1172, and the second connection component 131 includes: a first groove 1311 and a second groove 1312. The sizes or shapes of the first groove 1311 and the second groove 1312 are different. The size and shape of the first groove 1311 are the same as those of the first protrusion 1171, and the size and shape of the second groove 1312 are the same as those of the second protrusion 1172. In this way, it is ensured that the second protrusion 1172 and the first protrusion 1171 cannot be reversely placed in the first groove 1311 and the second groove 1312 at the same time.

[0081] In some other embodiments, the first connection component 117 and the second connection component 131 can also be processed into irregular shapes to achieve mating only at one angle. In addition, the number and form of the components that form the mating between the first connection component 117 and the second connection component can also be set to achieve that the first connection component 117 and the second connection component 131 can mate only at one angle. The first connection component 117 and the second connection component 131 can also be mated through other existing anti-fooling mechanisms, which will not be elaborated in detail here.

[0082] In some embodiments, as Figure 12 shown, from the second surface S2 to the first surface S1 of the fixture cover 13, the guiding hole 132 at least includes a first guiding hole section 1321 and a second guiding hole section 1322. The diameter of the first guiding hole section 1321 is larger than that of the second guiding hole section 1322, and the connection between the first guiding hole section 1321 and the second guiding hole section 1322 is beveled.

[0083] It should be noted that, in the above embodiments, sealing members, such as rubber rings, are also provided at the first guiding hole section 1321 and the second guiding hole section 1322 to ensure the sealing between the first guiding hole section 1321, the second guiding hole section 1322 and the perfusion member.

[0084] The beveled shape includes a conical shape and also includes other shapes that gradually decrease from large to small, such as an arc shape. It should be noted that the beveled shape provided in the embodiments of the present application is only used for a specific introduction of the embodiments and does not limit the present application.

[0085] In some embodiments, please refer to Figure 13 , Figure 13 which shows an exploded schematic view of a fixture provided by an embodiment of the present application. As Figure 13 shown, the fixing component 12 includes: a sliding piece 121 and a spring 122. A sliding groove 1181 is formed on the second surface F2 of the fixture 100 body. The sliding groove 1181 is opposite to the accommodating groove 111, and a spring hole 1182 is formed between the sliding groove 1181 and the accommodating groove 111 for installing the spring 122. The sliding piece 121 is U-shaped. A part of the sliding piece 121 is placed in the sliding groove 1181, and a part is placed in the accommodating groove 111. One end of the spring 122 is connected to the bottom of the U-shape of the sliding piece 121, and the other end of the spring 122 is connected to the fixture 100 body. When an external force is applied to the sliding piece 121, the sliding piece 121 moves in the sliding groove 1181 to facilitate placing the microfluidic chip 300 in the accommodating groove 111. After placing the microfluidic chip 300 and stopping applying the external force, the spring 122 resets the sliding piece 121 to clamp the microfluidic chip 300.

[0086] In some embodiments, please refer toFigure 14 , Figure 14 An exploded schematic diagram of a clamp provided in an embodiment of the present application is shown. Figure 14 As shown, the fixing assembly 12 includes: a spring piece 123. The spring piece 123 includes: a fixing member 1231 and a paddle 1232. A fixing groove 119 is provided on the second surface F2 of the clamp 100 body, and the sliding groove 1181 is opposite to the fixing groove 119. The fixing groove 119 is used to install the fixing member 1231, and the fixing member 1231 is fixed to the fixing groove 119 by screws. When an external force is applied to the paddle 1232, the paddle 1232 is deformed to facilitate the placement of the microfluidic chip 300 in the receiving groove 111. After the microfluidic chip 300 is placed and the external force is stopped, the paddle 1232 recovers by itself to clamp the microfluidic chip 300. The spring piece 123 can be made of SUS304, SUS316, beryllium copper or carbon steel with Teflon coating on the surface.

[0087] It should be noted that the embodiment of the fixing component 12 in the embodiment of the present application is only used to introduce the fixture 100 of the microfluidic chip 300 in detail, and other existing forms of fixing components are all within the scope of the embodiment of the present application.

[0088] An embodiment of the present application provides a perfusion device, which is used to install a syringe, and the syringe includes a container and a piston member. Two groups of syringes are installed in the same direction on the perfusion device, one of the two groups of syringes is a first syringe, and the other group is a second syringe, that is, when the two groups of syringes are installed on the perfusion device, the relative positions of the container and the piston member of each group of syringes are the same.

[0089] See also Figure 15 , Figure 15 The schematic diagram of the structure of a perfusion device provided in the embodiment of the present application is shown. Figure 15As shown, the perfusion device 100 includes: a base 33, a driving assembly 34, a slider assembly 35, a first clamping assembly 36 and a second clamping assembly 37. The driving assembly 34 is arranged on the base 33. The slider assembly 35 is slidably connected with the driving assembly 34, and the driving assembly 34 is used to drive the slider assembly 35 to move. The first clamping assembly 36 is installed on the slider assembly 35, and the first clamping assembly 36 is used to fix the receiving member 311 of at least one first perfusion device 31. The second clamping assembly 37 is installed on the base 33, and the second clamping assembly 37 is used to fix the receiving member 321 of at least one second perfusion device 32. Among them, the slider assembly 35 includes a first fixing member 354, and the first fixing member 354 is used to fix the piston member 322 of the second perfusion device 32, and / or the base 33 is provided with a second fixing member 331, and the second fixing member 331 is used to fix the piston member 312 of the first perfusion device 31. The driving assembly 34 drives the slider assembly 35 to move, driving one of the first syringe 31 and the second syringe 32 to achieve the function of pumping liquid and the other to achieve the function of injecting liquid. The bottom of the second fixing member 331 is provided with a waist-shaped hole, which can be adjusted forward and backward to adapt to syringes of different brands and models.

[0090] In some embodiments, the slider assembly 35 is provided with a first fixing member 354, and the first fixing member 354 is used to fix the piston member 322 of the second syringe 32, so that when the slider assembly 35 moves in the first direction, the piston member 322 of the second syringe 32 is pulled, and the receiving member 321 of the second syringe 32 is fixed on the base 33. When the slider assembly 35 moves in the first direction, the second syringe 32 realizes the function of pumping liquid. The receiving member 311 of the first syringe 31 is fixed on the slider assembly 35, and the piston member 312 of the first syringe 31 is supported by the second fixing member 331 on the base 33. When the slider assembly 35 moves in the first direction, it pushes the receiving member 311 of the first syringe 31, and the second syringe 32 realizes the function of injecting liquid.

[0091] In some embodiments, Figure 15 As shown, the first fixing member 354 is installed on the side of the slider assembly 35 facing the second direction, the piston member 322 of the second syringe 32 includes a protruding piece, and the first fixing member 354 is used to fix the protruding piece of the piston member 322 of the second syringe 32. In this way, when the slider assembly 35 pushes the piston member 322 of the second syringe 32 to move in the first direction, the relative positions of the slider assembly 35 and the piston member 322 of the second syringe 32 can be kept unchanged, thereby ensuring the accuracy of the movement of the piston member 322 of the second syringe 32 in the receiving member 321 of the second syringe 32, thereby improving the injection accuracy.

[0092] In some other embodiments, the piston member 312 of the first injector 31 is fixed to the second fixing member 331. When the slider assembly 35 pulls the receiving member 311 of the first injector 31 to move in the second direction, the first injector 31 realizes the liquid suction function. The receiving member 321 of the second injector 32 is fixed to the base 33. When the slider assembly 35 pushes the piston member 322 of the second injector 32 to move in the second direction, the liquid injection function is realized.

[0093] It should be noted that when the slider assembly 35 moves in the first direction, only the second fixing member 331 is provided and the first fixing member 354 is not provided, which can drive one of the first injector 31 and the second injector 32 to realize the liquid suction function and the other to realize the liquid injection function at the same time. When the slider assembly 35 moves in the second direction, only the first fixing member 354 is provided and the second fixing member 331 is not provided, which can also drive one of the first injector 31 and the second injector 32 to realize the liquid suction function and the other to realize the liquid injection function at the same time.

[0094] In the perfusion device provided by the embodiment of the present application, the first clamping assembly is connected to the slider assembly. The receiving member of the first injector is fixed to the first clamping assembly, the piston member of the first injector is fixed to the second fixing member of the base, the receiving member of the first injector is fixed to the second clamping assembly, the second clamping assembly is fixed to the base, and the piston member of the second injector is fixed to the second fixing member of the slider assembly. Therefore, when the slider assembly moves, it will drive the receiving member of the first injector to move and drive the piston member of the second injector to move, so that the two groups of injectors respectively realize the liquid injection function and the liquid suction function at the same time, reducing the number of perfusion devices required to realize the liquid injection function and the liquid suction function at the same time, saving space and equipment costs. At the same time, it also realizes equal or proportional liquid suction and liquid injection.

[0095] Since the first injector and the second injector in the embodiment of the present application are installed in the same direction, the two groups of injectors share the longitudinal space and reduce the required lateral space for installation. Therefore, the lateral space required for the perfusion device is reduced, thereby reducing the occupied area of the perfusion device. The needle heads face the same direction, which can make the liquid suction / liquid injection pipelines arranged more neatly.

[0096] In order to more clearly introduce the technical solution of the present application, the technical solution of the present application will be further introduced through specific embodiments below. It should be noted that the specific embodiment is used to expand the description of the technical solution of the present application, rather than limiting the present application.

[0097] In some embodiments, please refer to Figure 16 , Figure 16 which shows a partial structural schematic diagram of a perfusion device provided by the embodiment of the present application. As Figure 16As shown, the driving assembly 34 includes: a motor (not shown in the figure), a guide rod 341, and a driving lead screw 342. The base 33 includes: a main seat 332, a first support 333, and a second support 334. The first support 333 and the second support 334 are oppositely arranged on the main seat 332, and both ends of the guide rod 341 and the driving lead screw 342 are respectively installed on the first support 333 and the second support 334.

[0098] The motor is arranged on any one of the main seat 332, the first support 333, and the second support 334. The motor is used to drive the driving lead screw 342 to rotate. The driving lead screw 342 is also connected to the slider assembly 35, and is further used to drive the slider assembly 35 to move. A transmission assembly and a control circuit are also arranged in the base 33. The transmission assembly is used to connect the motor and the driving lead screw 342, and the control circuit is used to control the rotation of the motor.

[0099] In some embodiments, as Figure 16 shown, the slider assembly 35 further includes a slider member 351, a connecting member 352, and a sliding seat member 353. The sliding seat member 353 is slidably connected to the guide rod 341 and the driving lead screw 342. The sliding seat member 353 is also connected to the connecting member 352, and the slider member 351 is installed on the connecting member 352.

[0100] In some embodiments, please refer to Figure 17 , Figure 17 which shows an exploded schematic view of a perfusion device provided by an embodiment of the present application. As Figure 17 shown, the connecting member 352 is provided with a cavity. The sliding seat member 353 is installed in the cavity of the connecting member 352. Both the sliding seat member 353 and the connecting member 352 are provided with through holes corresponding to the guide rod 341 and the driving lead screw 342. The guide rod 341 and the driving lead screw 342 pass through the through holes of the sliding seat member 353 and the through holes of the connecting member 352, thereby allowing the slider assembly 35 to linearly move along a first direction or a second direction on the driving assembly 34.

[0101] In some embodiments, please refer to Figure 18 , Figure 18 which shows a structural schematic view of a first clamping assembly provided by an embodiment of the present application. As Figure 18 shown, the first clamping assembly 36 includes: a first supporting member 361, a first cover member 362, and a first fixing stud 363. The first supporting member 361 is connected to the first cover member 362. The receiving member 311 of the first perfusion device 31 is installed between the first supporting member 361 and the first cover member 362. The first fixing stud 363 is used to keep the first supporting member 361 and the first cover member 362 fixed.

[0102] The first support member 361 is provided with a plurality of V-shaped mounting grooves, and each mounting groove is used to mount a receiving member 311 of a first injector 31. After the receiving member 311 of the first injector 31 is placed into the mounting groove, the first cover member 362 is covered. In this way, the stability among the three can be improved to reduce shaking.

[0103] Before mounting the first injector 31 on the first clamping assembly 36, first separate the first cover member 362 and the first support member 361, then mount the receiving member 311 of the first injector 31 on the first support member 361, and then mount the first cover member 362 on the first support member 361 and fasten the three with the first fixing stud 363. In this way, the injector can be stably clamped on the first clamping assembly 36.

[0104] The diameter of the upper section of the first fixing stud 363 is larger than that of the lower section. The first cover member 362 is provided with a through hole to allow the lower section of the first fixing stud 363 to pass through and clamp the upper section of the first threaded stud. The lower section of the first fixing stud 363 and the first support member 361 are also provided with mutually matching threads and threaded grooves to fix the first fixing stud 363 on the first support member 361.

[0105] In some embodiments, such as Figure 18 shown, the first clamping assembly 36 further includes a handle member 364, and the handle member 364 is connected to the first cover member 362. The user can lift the first clamping assembly 36 through the handle member 364.

[0106] Such as Figure 17 shown, the first support member 361 is connected to the slider member 351 by screws. The first support member 361 and the slider member 351 are provided with a carry pin 38, and a certain space is reserved between the two through the carry pin 38. The first cover member 362 is provided with screw through holes, and the screw through holes of the first cover member 362 facilitate the user to loosen and tighten the screws for connecting the first support member 361 and the slider member 351.

[0107] In some embodiments, such as Figure 18 shown, the first clamping group further includes a hook member 365 and a first positioning member 366. The slider assembly 35 further includes a buckling member 355, and the buckling member 355 is movably connected to the hook member 365. The upper part of the slider member 351 is a protrusion, and the width of the protrusion in the third direction is the same as the distance between the two first positioning members 366 of the first clamping assembly 36. The two first positioning members 366 can cooperate with the protrusion, and then buckle the buckling member 355 to the hook member 365 to ensure the stability between the first clamping assembly 36 and the slider assembly 35.

[0108] In some embodiments, please refer to Figure 19 , Figure 19Shows a schematic structural diagram of a first clamping assembly provided by an embodiment of the present application. As Figure 19 shown, the first clamping assembly 36 further includes a first clamping plate member 367 and a first locking member 368. The first locking member 368 is connected to the first supporting member 361, and the first clamping plate member 367 is connected to the first locking member 368. The first clamping plate member 367 is used to fix the accommodating member 311 of the first injector 31 to the first supporting member 361. One end of the accommodating member 311 of the first injector 31 has a raised piece, and through the first clamping plate member 367 and the first supporting member 361, the raised piece is clamped between the two to further improve the stability between the first injector 31 and the first clamping assembly 36.

[0109] Exemplarily, the first locking member 368 is connected to the first supporting member 361, the first locking member 368 is rotatable, and the first locking member 368 and the first clamping plate member 367 are provided with mutually cooperating threads and thread grooves. The first locking member 368 adjusts the tightness between it and the first clamping plate member 367 by rotation, thereby adjusting the tightness between the first clamping plate member 367 and the first supporting member 361 to facilitate the taking and placing of the first injector 31.

[0110] In some embodiments, please refer to Figure 20 , Figure 20 Shows a schematic structural diagram of a second clamping assembly provided by an embodiment of the present application. As Figure 20 shown, the second clamping assembly 37 includes: a second supporting member 371, a second cover plate member 372, and a second fixing stud 373. The second supporting member 371 is connected to the second cover plate member 372, and the accommodating member 321 of the second injector 32 is installed between the second supporting member 371 and the second cover plate member 372.

[0111] The second supporting member 371 is connected to the second cover plate member 372, the accommodating member 321 of the second injector 32 is installed between the second supporting member 371 and the second cover plate member 372, and the second fixing stud 373 is used to keep the second supporting member 371 and the second cover plate member 372 fixed.

[0112] The second supporting member 371 is provided with a plurality of V-shaped installation grooves, and each installation groove is used to install two accommodating members 321 of the second injector 32. After the accommodating member 321 of the second injector 32 is placed into the installation groove, the second cover plate member 372 is covered. In this way, the stability among the three can be improved to reduce shaking.

[0113] Before installing the second infuser 32 on the second clamping assembly 37, first separate the second cover plate member 372 and the second supporting member 371, then install the accommodating member 321 of the second infuser 32 on the second supporting member 371, and then install the second cover plate member 372 on the second supporting member 371, and fasten the three together through the second fixing stud 373. In this way, the infuser can be stably clamped on the second clamping assembly 37.

[0114] The diameter of the upper section of the second fixing stud 373 is larger than that of the lower section. The second cover plate member 372 is provided with a through hole to allow the lower section of the second fixing stud 373 to pass through and clamp the upper section of the second threaded stud. A mutually matching thread and thread groove are also provided between the lower section of the second fixing stud 373 and the second supporting member 371 to fix the second fixing stud 373 to the second supporting member 371.

[0115] In some embodiments, the second clamping assembly 37 further includes a second fixing stud 374. The base 33 includes a clamping assembly mounting plate 335. The second fixing stud 374 is used to fix the second supporting member 371 on the clamping assembly mounting plate 335. As Figure 17 shown, a carry pin 38 is provided between the second supporting member 371 and the clamping assembly mounting plate 335 to space the second supporting member 371 and the clamping assembly mounting plate 335 apart.

[0116] In some embodiments, the second cover plate member 372 is provided with a through hole to facilitate the user to operate the second fixing stud 374 through the through hole.

[0117] In some embodiments, the second clamping assembly 37 further includes a support frame member 375 and a indexing pin 376 member.

[0118] In some embodiments, please refer to Figure 21 , Figure 21 which shows a schematic structural diagram of a second clamping assembly provided by an embodiment of the present application. As Figure 21 shown, the second clamping assembly 37 further includes: a second clamping plate member 377 and a second locking member 378. The second locking member 378 is connected to the second supporting member 371, the second clamping plate member 377 is connected to the second locking member 378, and the second clamping plate member 377 is used to fix the accommodating member 321 of the second infuser 32 to the second supporting member 371.

[0119] Exemplarily, the second locking member 378 is connected to the second supporting member 371. The second locking member 378 is rotatable. The second locking member 378 and the second clamping plate member 377 are provided with mutually cooperating threads and thread grooves. The second locking member 378 adjusts the tightness between it and the second clamping plate member 377 by rotation, thereby adjusting the tightness between the second clamping plate member 377 and the second supporting member 371 to facilitate the taking and placing of the second injector 32. One end of the accommodating member 321 of the second injector 32 has a raised piece, and the raised piece is clamped between the second clamping plate member 377 and the second supporting member 371 to further improve the stability between the second injector 32 and the second clamping assembly 37.

[0120] In some embodiments, refer to Figure 22 , Figure 22 which shows a schematic structural diagram of a combination of a first clamping assembly and a second clamping assembly provided by an embodiment of the present application. As Figure 22 shown, the second clamping assembly 37 further includes: a support frame member 375 and a indexing pin 376. The support frame member 375 is connected to the second cover plate member 372, and the indexing pin 376 is connected to the second cover plate member 372.

[0121] In some embodiments, as Figure 22 shown, the first clamping assembly 36 includes: a first positioning member 366. The first positioning member 366 is provided with a positioning hole. The second clamping assembly 37 further includes an indexing pin 376. The indexing pin 376 forms a fit with the positioning hole. The indexing pin 376 is installed on the support frame. When the first clamping assembly 36 and the second clamping assembly 37 are combined, the support frame member 375 is used to support the first clamping assembly 36.

[0122] Exemplarily, Figure 22As shown, the first clamping assembly 36 and the second clamping assembly 37 can be stacked together. After the first perfusion device 31 and the second perfusion device 32 are respectively installed on the first clamping assembly 36 and the second clamping assembly 37, they are first stacked into an integral body and then installed on the perfusion device 100. Among them, when stacking, the first clamping assembly 36 is on the top and the second clamping assembly 37 is at the bottom. The support member 375 of the second clamping assembly 37 separates the first clamping assembly 36 and the second clamping assembly 37 and provides support for the first clamping assembly 36. Index pins 376 are installed at both ends of the support member 375. The index pins 376 have retractable pin posts, and the pin posts cooperate with the positioning holes of the first positioning member 366; when the first clamping assembly 36 and the second clamping assembly 37 are stacked together, the pin posts extend and snap into the positioning holes to maintain the stability between the first clamping assembly 36 and the second clamping assembly 37; when the first clamping assembly 36 and the second clamping assembly 37 need to be separated, the pin posts contract and withdraw from the positioning holes, so that the first clamping assembly 36 can be taken away from the second clamping assembly 37. After the first clamping assembly 36 and the second clamping assembly 37 are stacked, the second clamping assembly 37 is first fixed on the base 33, then the one installed on the slider assembly 35 is taken away from the first clamping assembly 36, and then the first clamping assembly 36 and the slider assembly 35 are fixed by the fastening member 355 and the hook member 365.

[0123] In some embodiments, as Figure 22 shown, the first clamping assembly 36 further includes: a handle 364, and the handle 364 is connected to the first cover plate 362 to facilitate the user to lift and place the first clamping assembly 36.

[0124] Through the stacking mechanism of the first clamping assembly 36 and the second clamping assembly 37, it is convenient for the user to operate two groups of perfusion devices respectively used to realize the liquid injection function and the liquid extraction function at the same time, thus avoiding the situation of mixing and misusing the perfusion devices in a biological reaction.

[0125] In some embodiments, the second clamping assembly 37 further includes a second positioning member. When multiple second clamping assemblies 37 are stacked together, the second positioning member is used to cooperate with the index pin 376.

[0126] In some embodiments, please refer to Figure 23 , Figure 23 which shows a schematic structural diagram of a first fixing member provided by an embodiment of the present application. As Figure 23 shown, the first fixing member 354 includes: a first fixing groove 3541 and a first limiting piece 3542. Combining Figure 17, the first fixing groove 3541 is formed on one side of the first fixing member 354 close to the slider member 351, and the first limiting piece 3542 is arranged on the other side of the first fixing member 354, and these two sides are opposite to each other. The first fixing groove 3541 is used to place the protruding piece of the piston member 322 of the second syringe 32. The first limiting piece 3542 is provided with a channel, for example, the channel is V-shaped, and the area of the channel of the first limiting piece 3542 is smaller than that of the protruding piece, so as to clamp the protruding piece of the piston member 322 of the second syringe 32, and then pull the accommodating member 321 of the second syringe 32 to move in the first direction. It should be noted that the first limiting piece 3542 and the first fixing member 354 can be integrally formed or separately formed and then combined.

[0127] In some embodiments, please refer to Figure 24 , Figure 24 which shows a schematic structural diagram of a second fixing member provided by an embodiment of the present application. As Figure 24 shown, the second fixing member 331 includes: a second fixing groove 3311 and a second limiting piece 3312. The second fixing groove 3311 is formed on the side of the second fixing member 331 facing the second direction, and the second limiting piece 3312 is also arranged on the side of the second fixing member 331 facing the second direction. The second fixing groove 3311 is used to place the protruding piece of the piston member 312 of the first syringe 31. The second limiting piece 3312 is provided with a channel, for example, the channel is V-shaped, and the area of the channel of the second limiting piece 3312 is smaller than that of the protruding piece, so as to clamp the protruding piece of the piston member 312 of the first syringe 31, and then prevent the piston member 312 of the first syringe 31 from moving in the second direction along with the accommodating member 311 of the first syringe 31. It should be noted that the second limiting piece 3312 and the second fixing member 331 can be integrally formed or separately formed and then combined.

[0128] The first fixing member 354 and the second fixing member 331 are respectively used to fix the piston part of the second perfusion member and the piston part of the first perfusion member. In this way, it can be ensured that during the movement of the accommodating member 311 of the first syringe 31 and the piston part 322 of the second syringe 32, the piston part of the first perfusion member and the piston part of the second perfusion member are always fixed, so as to ensure the movement accuracy of the piston member 312 of the first syringe 31 in the accommodating member 311 of the first syringe 31 and the movement accuracy of the piston member 322 of the second syringe 32 in the accommodating member 321 of the second syringe 32, and then improve the liquid change accuracy.

[0129] In some embodiments, please refer to Figure 25 , Figure 25 which shows a schematic structural diagram of a fixture provided by an embodiment of the present application. As Figure 25As shown, the fixture 100 further includes a tray handle 15 and a support plate 16. The tray handle 15 and the support plate 16 are both installed on the tray 14. Multiple card slots are provided on the support plate 16, and each card slot is used to stabilize a connecting pipe 41.

[0130] In some embodiments, please refer to Figure 26 , Figure 26 which shows a schematic structural diagram of a biological reaction system provided by an embodiment of the present application. As Figure 26 shown, the biological reaction system 400 further includes a first accommodation cabinet 42 and a second accommodation cabinet 43. The first accommodation cabinet is used to place the perfusion device 300, and the second accommodation cabinet 43 is used to place the fixture 100. Among them, the fixture 100 holds a microfluidic chip 200 as Figure 2 shown. The first accommodation cabinet 42 and the second accommodation cabinet 43 have functions such as temperature control, humidity control, dust prevention, and light supply, so as to provide a good reaction environment for the biological reaction.

[0131] In some embodiments, as Figure 26 shown, the first accommodation cabinet 42 further includes a wire bundling buckle 421, and the wire bundling buckle 421 is used to bundle the connecting pipes 41 to prevent the connecting pipes 41 from being scattered disorderly.

[0132] In some embodiments, as Figure 26 shown, the second accommodation cabinet 43 further includes: a partition slot 431 and a bearing plate 432. The partition slots 431 are respectively arranged on two opposite side walls of the cavity of the second accommodation cabinet 43, and every two corresponding partition slots 431 are used to place the bearing plate 432. The bearing plate 432 is used to place the fixture 100.

[0133] In some embodiments, a light source can be placed on the bearing plate 432 above the fixture 100, and the light source is used to provide necessary lighting conditions for the microfluidic chip 200 in the fixture 100 to complete the biological reaction.

[0134] The bearing plate 432 can be made of a transparent material. For example, glass and acrylic plates. In this way, an observation device can be placed on the bearing plate 432 below the fixture 100 to observe the microfluidic chip 200 in the fixture 100 through the bearing plate 432 on which the fixture 100 is placed.

[0135] In some embodiments, please refer to Figure 27 , Figure 27 which shows a schematic structural diagram of a microfluidic chip provided by an embodiment of the present application. As Figure 27 shown, a bottom opening 34 is provided at the bottom of the microfluidic chip 300, and the bottom opening 34 is connected to a cavity 32 as Figure 2 shown. The bottom opening 34 is used to observe the process of the biological reaction in the cavity 32.

[0136] In some embodiments, such as Figure 7 the tray 14 shown, the bottom of the receiving groove of the tray 14 and the area corresponding to the bottom opening 34 shown as Figure 27 such are made of a transparent material, so as to observe the microfluidic chip 200 in the fixture 100 through this area.

[0137] In some embodiments, please refer to Figure 28 , Figure 28 which shows a schematic cross-sectional view of a capillary tube provided by an embodiment of the present application. As shown in Figure 28 , the first opening 3311 of the micropore 331 of the capillary 33 is larger than the second opening 3312 of the micropore 331. The first opening 3311 is the opening corresponding to the outside of the capillary 33 of the micropore 331, and the second opening 3312 is the opening corresponding to the inside of the capillary of the micropore. The first opening 3311 gradually narrows towards the second opening 3312. When processing the pore wall of the micropore 331, due to process deviation, the opening size of the micropore 331 is insufficient. Through the above opening mechanism, when the micropore 331 is formed on the capillary 33, it gradually narrows from the outside to the inside, ensuring the accuracy of the second opening 3312.

[0138] In some embodiments, the minimum width range of the second opening 3312 is: 1 μm - 20 μm. Specifically, the minimum width range of the second opening 3312 is 3 μm. The minimum width range of the second opening 3312 is the width at the narrowest part of the second opening 3312. For example, if the second opening 3312 is rectangular, the wide side of the rectangle is the narrowest part of the second opening 3312, and the length of the wide side of the rectangle is the minimum width range of the second opening 3312. The second opening 3312 is for the nutrients in the liquid in the cavity 32 shown as Figure 2 such to slowly flow out, creating a capillary-like culture environment for better culturing of biological cells.

[0139] In some embodiments, in order to improve production efficiency and accuracy, the microfluidic chip is integrally formed by photocuring 3D printing.

[0140] In a hardware implementation, the division between the functional modules / units mentioned in the above description does not necessarily correspond to the division of physical modules; for example, a single physical module may have multiple functions, or a function or step may be executed by the cooperation of several physical modules. Some or all of the physical modules may be implemented as software executed by a processor, such as a central processing unit, a digital signal characteristic electrical signal processor, or a microprocessor, or implemented as hardware, or implemented as an integrated circuit, such as an application specific integrated circuit. Such software may be distributed on a computer-readable medium, which may include a computer storage medium (or non-transitory medium) and a communication medium (or transitory medium). As is well known to those of ordinary skill in the art, the term computer storage medium includes volatile and non-volatile, removable and non-removable media implemented in any method or technology for storing information such as computer-readable instructions, data structures, program modules, or other data. Computer storage media includes, but is not limited to, RAM, ROM, EEPROM, flash memory or other memory technologies, CD-ROM, digital versatile disks (DVDs) or other optical disk storage, magnetic cassettes, tapes, magnetic disk storage or other magnetic storage devices, or any other medium that can be used to store the desired information and can be accessed by a computer. In addition, it is well known to those of ordinary skill in the art that communication media typically contains computer-readable instructions, data structures, program modules, or other data in a modulated data signal such as a carrier wave or other transmission mechanism, and may include any information delivery media.

[0141] The preferred embodiments of the present application have been described above with reference to the accompanying drawings, and thus do not limit the scope of the rights of the present application. Any modifications, equivalent replacements, and improvements made by those skilled in the art without departing from the scope and essence of the present application shall be within the scope of the rights of the present application.

Claims

1. A biological reaction system, characterized in that, the biological reaction system includes: a microfluidic chip, the microfluidic chip includes a chip body, a cavity and a capillary, the capillary is located in the cavity, and a liquid inlet and a liquid outlet are provided on the third surface of the chip body; a fixture, the fixture includes: a fixture body and a fixing component, a receiving groove is provided on the first surface of the fixture body, the second surface of the fixture body is opposite to the first surface, the receiving groove is used for placing the microfluidic chip, the fixture body is further provided with a liquid injection flow channel and a liquid extraction flow channel, the first end of the liquid injection flow channel is used for conveying liquid to the liquid inlet, the cavity is located at the opening of the chip body and the liquid discharged from the liquid outlet flows into the receiving groove, and the receiving groove is communicated with the first end of the liquid extraction flow channel; the fixing component is arranged on the fixture body and is used for fixing the microfluidic chip in the receiving groove; a perfusion device, the perfusion device is respectively connected to the second end of the liquid injection flow channel and the second end of the liquid extraction flow channel, and is used for replacing the liquid in the microfluidic chip.

2. The biological reaction system according to claim 1, characterized in that, the opening of the cavity is located on the second surface of the chip body, and the second surface of the chip body is opposite to the first surface; wherein, the liquid inlet and the liquid outlet are further provided on the third surface of the chip body, the capillary can replace the liquid through the liquid inlet and the liquid outlet, and the third surface of the chip body is perpendicular to the first surface and the second surface of the chip body.

3. The biological reaction system according to claim 2, characterized in that, at least one micropore is provided in the capillary, the first opening of the micropore is larger than the second opening of the micropore, the first opening is the opening corresponding to the outside of the capillary of the micropore, the second opening is the opening corresponding to the inside of the capillary of the micropore, and the first opening gradually shrinks towards the second opening.

4. The biological reaction system according to claim 1, characterized in that, a communication groove is further provided on the first surface of the fixture body, and the liquid flowing out of the microfluidic chip flows into the receiving groove through the communication groove.

5. The biological reaction system according to claim 1, characterized in that, an observation through hole is further provided between the first surface and the second surface of the fixture body, and the observation through hole is communicated with the receiving groove.

6. The biological reaction system according to claim 1, characterized in that, an anti-overflow groove is further provided on the first surface of the fixture body, the anti-overflow groove is connected to the second end of the liquid extraction flow channel, and the anti-overflow groove and the receiving groove are in a stepped shape.

7. The biological reaction system according to claim 6, characterized in that, the height difference between the anti-overflow groove and the receiving groove is: 0.5 mm - 3 mm; and / or, the distance between the side wall of the anti-overflow groove and the second end of the liquid extraction flow channel is: 2 mm - 5 mm.

8. The biological reaction system according to claim 1, characterized in that, a first connection component is provided on the second surface of the fixture body, the fixture further includes a fixture cover, and the fixture cover includes: A second connecting component, which is used to connect with the first connecting piece of the fixture body; Two guiding holes, which communicate the second surface and the first surface of the fixture cover. The second surface and the first surface of the fixture cover are opposite to each other, and the two guiding holes respectively correspond to the first end of the liquid injection flow channel and the first end of the liquid extraction flow channel.

9. The biological reaction system according to any one of claims 1-8, characterized in that the perfusion device includes: a base; a driving component, which is arranged on the base; a slider component, which is slidably connected with the driving component, and the driving component is used to drive the slider component to move; a first clamping component, which is installed on the slider component, and the first clamping component is used to fix the accommodating parts of at least one first perfusion device; a second clamping component, which is installed on the base, and the second clamping component is used to fix the accommodating parts of at least one second perfusion device; wherein, the slider component includes a first fixing piece, which is used to fix the piston part of the second perfusion device, and / or, the base is provided with a second fixing piece, which is used to fix the piston part of the first perfusion device; the driving component drives the slider component to move, driving one of the first perfusion device and the second perfusion device to realize the liquid extraction function, and the other to realize the liquid injection function.

10. The biological reaction system according to claim 9, characterized in that the driving component includes: a motor, a guiding rod and a driving lead screw. The base includes a main seat, a first support and a second support. The first support and the second support are oppositely arranged on the main seat. The two ends of the guiding rod and the driving lead screw are respectively installed on the first support and the second support. The motor is arranged on any one of the main seat, the first support and the second support. The motor is used to drive the driving lead screw to rotate, and the driving lead screw is also connected with the slider component, and is further used to drive the slider component to move; the slider component further includes a slider piece, a connecting piece and a sliding seat piece. The sliding seat piece is slidably connected with the guiding rod and the driving lead screw. The sliding seat piece is also connected with the connecting piece, and the slider piece is installed on the connecting piece.

11. The biological reaction system according to claim 9, characterized in that the first clamping component includes: a first supporting piece and a first cover plate piece. The first supporting piece is connected with the first cover plate piece, and the accommodating part of the first perfusion device is installed between the first supporting piece and the first cover plate piece; the second clamping component includes: a second supporting piece and a second cover plate piece. The second supporting piece is connected with the second cover plate piece, and the accommodating part of the second perfusion device is installed between the second supporting piece and the second cover plate piece.

12. The biological reaction system according to any one of claims 1-8, characterized in that the fixture further includes a tray for placing the fixture body.

13. The biological reaction system according to any one of claims 1-8, characterized in that the microfluidic chip is integrally formed by photocuring 3D printing.

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