Method for artificial propagation of longfin dace

Abstract

The present application relates to the field of aquaculture technology, and more particularly to a long filament schizothorax artificial breeding method, which comprises the following steps: (1) temporarily raising the captured wild long filament schizothorax in an ecological simulation pool, and then transferring the long filament schizothorax into a special breeding pool for intensive cultivation; (2) selecting parent fish and injecting oxytocin; (3) artificially inseminating the female fish about to give birth, and then putting the parent fish into a postpartum cultivation pool for postpartum cultivation, hatching long filament schizothorax fertilized eggs, and obtaining long filament schizothorax fry; and (4) primary cultivation of the long filament schizothorax fry until the body length is greater than 2 cm. The present application includes the processes of oxytocin injection, spawning, insemination, hatching and cultivation of fry of long filament schizothorax, has a high hatching rate of fertilized eggs, and fast growth rate of fry, and provides a technical basis for artificial breeding of long filament schizothorax.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of aquaculture, in particular to a method for artificial propagation of Schizothorax dolichonema. BACKGROUND

[0002] Schizothorax dolichonema belongs to Cypriniformes, Cyprinidae, Schizothoracinae and Schizothorax, and likes to live in flowing water environment. It is one of the main fishing objects in the upper reaches of the Yangtze River, mainly distributed in the Lancang River, Jinsha River and Yalong River. The body of Schizothorax dolichonema is slightly long and slightly flat. The snout is blunt and round, the mouth is inferior and slightly arc-shaped, the lower jaw has sharp horny edges, there are two pairs of barbels with almost equal length, both of which are larger than the eye diameter, the dorsal fin spine is strong, the body is covered with fine scales, the chest has obvious scales, and the snout of male fish has a pearl star in the breeding season. The back of the body is blue-gray or gray-brown, the abdomen is white, and the tail fin is slightly red. It usually lives in wide valley rivers with slow water flow in mountainous areas, and the river bottom is mostly gravel with clear water. It feeds on diatoms, blue-green algae, green algae and some benthic invertebrates. It grows slowly, and female fish generally reach sexual maturity at the age of 4. They often gather and make short-distance migration during the breeding season, and the breeding period is mostly in March to April.

[0003] Schizothorax dolichonema is also an important economic fish. In recent years, with the disturbance of human factors such as overfishing, environmental pollution and tributary small hydropower development, the natural resource status of Schizothorax dolichonema has been seriously affected. It is imperative to realize its artificial large-scale breeding and strengthen the protection of Schizothorax dolichonema germplasm resources as soon as possible. SUMMARY

[0004] The present application aims to provide a method for artificial propagation of Schizothorax dolichonema.

[0005] In order to achieve the above-mentioned purpose of the application, the present application provides the following technical scheme:

[0006] The present application provides a method for artificial propagation of Schizothorax dolichonema, comprising the following steps:

[0007] (1) The captured wild Schizothorax dolichonema is first temporarily raised in an ecological simulation pool, and then transferred to a specialized culture pool for intensive culture;

[0008] (2) Selecting parent fish and injecting oxytocin;

[0009] (3) Artificial insemination is performed on the female fish about to give birth, and then the parent fish is respectively put into a postpartum culture pool for postpartum culture, so as to hatch the Schizothorax dolichonema fertilized eggs and obtain Schizothorax dolichonema fry;

[0010] (4) The Schizothorax dolichonema fry is cultured in the initial stage until the body length is greater than 2 cm.

[0011] Preferably, the water flow rate in the ecological simulation tank of step (1) is 2.5-3.5 m / s; and the ecological simulation tank contains 35-45% (v / v) of the water from the natural habitat.

[0012] Preferably, the water flow rate in the ecological simulation tank of step (1) is 2.5-3.5 m / s; and the ecological simulation tank contains 35-45% (v / v) of the water from the natural habitat.

[0013] Preferably, the water flow rate in the ecological simulation tank of step (1) is 2.5-3.5 m / s; and the ecological simulation tank contains 35-45% (v / v) of the water from the natural habitat.

[0014] Preferably, the water flow rate in the ecological simulation tank of step (1) is 2.5-3.5 m / s; and the ecological simulation tank contains 35-45% (v / v) of the water from the natural habitat.

[0015] Preferably, the water flow rate in the ecological simulation tank of step (1) is 2.5-3.5 m / s; and the ecological simulation tank contains 35-45% (v / v) of the water from the natural habitat.

[0016] Preferably, the water flow rate in the ecological simulation tank of step (1) is 2.5-3.5 m / s; and the ecological simulation tank contains 35-45% (v / v) of the water from the natural habitat.

[0017] Preferably, the water flow rate in the ecological simulation tank of step (1) is 2.5-3.5 m / s; and the ecological simulation tank contains 35-45% (v / v) of the water from the natural habitat.

[0018] Preferably, the water flow rate in the ecological simulation tank of step (1) is 2.5-3.5 m / s; and the ecological simulation tank contains 35-45% (v / v) of the water from the natural habitat.

[0019] Preferably, the water flow rate in the ecological simulation tank of step (1) is 2.5-3.5 m / s; and the ecological simulation tank contains 35-45% (v / v) of the water from the natural habitat.

[0020] Compared with the prior art, the application has the following beneficial effects:

[0021] 1、The wild long filament schizothoracn fish is first temporarily raised in the ecological simulation pool which contains more biont water, the purpose is to simulate the outdoor natural environment, avoid the long term stress state of the long filament schizothoracn fish caused by the too large change of growth environment, and affect the normal growth of the long filament schizothoracn fish, then the long filament schizothoracn fish is transferred into the special raising pool for intensive cultivation, the special raising pool contains a small amount of biont water, so that the long filament schizothoracn fish can gradually adapt to the artificial cultivation environment. The special environment of the biont water is more suitable for the hatching of the fertilized eggs, so the biont water is directly used for the hatching process in the fertilized egg hatching process.

[0022] 2、Under natural conditions, the parent fish of the long filament schizothoracn fish will gather in the river section with rapid water flow when the breeding season comes, and the water level of the river will suddenly rise and the flow rate will accelerate to form bubble whirlpool water and other natural conditions. These external ecological conditions act on the central nervous system of the long filament schizothoracn fish through the external receptors (vision, touch, lateral line) of the parent fish, stimulate the hypothalamus to release GnRH (gonadotropin releasing hormone), and then trigger the pituitary of the male and female long filament schizothoracn fish to secrete a large amount of GtH (gonadotropin), which reaches the gonads through blood circulation. After the gonads are affected by GtH (gonadotropin), they develop and mature rapidly and start to ovulate. Under artificial breeding conditions, the parent fish lack this external stimulation, and the present application uses artificial oxytocin for induction, which has better induction effect than traditional oxytocin.

[0023] 3、The method of the present application includes the processes of induction, spawning, fertilization, hatching and cultivation of fry of the long filament schizothoracn fish, the hatching rate of the fertilized eggs is high, and the growth rate of the fry is fast, which provides a technical basis for artificial propagation of the long filament schizothoracn fish. BRIEF DESCRIPTION OF DRAWINGS

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the following embodiment or prior art description will be briefly introduced. Obviously, the drawings in the following description are only embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of the provided drawings.

[0025] Figure 1 The process of injecting oxytocin into the parent fish of the long filament schizothoracn fish;

[0026] Figure 2 The fry of the long filament schizothoracn fish ready to be put into the special raising pool (put into the pond). DETAILED DESCRIPTION

[0027] The present application provides an artificial propagation method of the long filament schizothoracn fish, which comprises the following steps:

[0028] (1) The wild long filament schizothoracn fish is first temporarily raised in the ecological simulation pool which contains more biont water, the purpose is to simulate the outdoor natural environment, avoid the long term stress state of the long filament schizothoracn fish caused by the too large change of growth environment, and affect the normal growth of the long filament schizothoracn fish, then the long filament schizothoracn fish is transferred into the special raising pool for intensive cultivation;

[0029] (2) selecting the parent fish and injecting oxytocin;

[0030] (3) artificially inseminating the parturient female fish, and then putting the parent fish into postpartum cultivation ponds for postpartum cultivation, so as to hatch the long shihuchiai fertilized eggs and obtain long shihuchiai fry;

[0031] (4) primary cultivation of the long shihuchiai fry until the body length is greater than 2 cm.

[0032] In the present application, the water flow rate in the ecological simulation pool in step (1) is 2.5-3.5 m / s; preferably 3 m / s.

[0033] In the present application, the ecological simulation pool in step (1) contains 35-45% (v / v) of the biogenic water; preferably 37-43% (v / v); further preferably 39-41% (v / v); and more preferably 40% (v / v).

[0034] In the present application, the water flow rate in the specialized pool in step (1) is 1-2 m / s; preferably 1.5 m / s.

[0035] In the present application, the specialized pool in step (1) contains 15-20% (v / v) of the biogenic water; preferably 16-19% (v / v); further preferably 17-18% (v / v); and more preferably 18% (v / v).

[0036] In the present application, the temporary cultivation time in step (1) is 8-12 h; preferably 9-11 h; and further preferably 10 h.

[0037] In the present application, the feed for the intensive cultivation in step (1) is: breadworms, fish meal, chicken liver powder, fly larva powder, earthworm powder and maifanshi powder, with a mass ratio of 20-26:16-20:6-10:5-9:3-5:2-4; preferably 21-25:17-19:7-9:6-8:4:3; further preferably 22-24:18:8:7:4:3; and more preferably 23:18:8:7:4:3.

[0038] In the present application, the oxytocin injection per female fish in step (2) is: luteinizing hormone releasing hormone analogue 8-12 μg + chorionic gonadotropin 250-350 IU + domperidone 5-7 μg, and the injection per male fish is halved; preferably, the injection per female fish is: luteinizing hormone releasing hormone analogue 9-11 μg + chorionic gonadotropin 270-330 IU + domperidone 6 μg, and the injection per male fish is halved; further preferably, the injection per female fish is: luteinizing hormone releasing hormone analogue 10 μg + chorionic gonadotropin 290-310 IU + domperidone 6 μg, and the injection per male fish is halved; more preferably, the injection per female fish is: luteinizing hormone releasing hormone analogue 10 μg + chorionic gonadotropin 300 IU + domperidone 6 μg.

[0039] In the present application, the water flow rate in the postpartum incubation tank in step (3) is 1-2 m / s; preferably, 1.5 m / s.

[0040] In the present application, the postpartum incubation tank in step (3) contains 35-45% (v / v) of the biogenic water; preferably, 37-43% (v / v); further preferably, 39-41% (v / v); more preferably, 40% (v / v).

[0041] In the present application, the feed for the postpartum incubation in step (3) is: fish meal, shrimp shell powder, yellow mealworm, black soldier fly larvae, fish premix, fructus amomi roxburghii extract, radix astragali extract, stemona extract, and sophora flavescens extract, with a mass ratio of 18-24:6-10:14-16:5-9:3-5:2-4:4-8:3-5:2-4; preferably, 19-23:7-9:15:6-8:4:3:5-7:4:3; further preferably, 20-22:8:15:7:4:3:6:4:3; more preferably, 21:8:15:7:4:3:6:4:3.

[0042] In the present application, the temperature for the incubation in step (3) is 18.3-21.1℃; preferably, 18.5-20.0℃; further preferably, 19.0-19.5℃; more preferably, 19.5℃.

[0043] In the present application, the time for the incubation in step (3) is 15-19 d; preferably, 16-18 d; further preferably, 17 d.

[0044] In the present application, the biogenic water is used for the incubation in step (3).

[0045] In the present application, the feed for the initial incubation in step (4) is: rotifer and nauplii of copepod, with a mass ratio of 1-3:3-1; preferably, 1:1.

[0046] In the present application, the fish premix is purchased from Zhuhai Winnow Aquaculture Co., Ltd.

[0047] The technical solutions provided by the present application will be described in detail below in combination with examples, but they should not be understood as limiting the scope of protection of the present application.

[0048] Example 1

[0049] A filament schizothoracn fish artificial breeding method, the steps are as follows:

[0050] (1) From March 2022 to the Longkai opening reservoir area of Jinshajiang River, wild filament schizothoracn fish was captured, mainly using three-layer gill nets and traps, and the captured wild filament schizothoracn fish was disinfected with povidone-iodine, and the severely injured ones were treated with intramuscular injection of penicillin (10,000 IU / kg) 2 ml and erythromycin ointment for wound dressing; and Jinshajiang River water was collected as the source water;

[0051] (2) The captured wild filament schizothoracn fish was temporarily raised in an ecological simulation pool (containing 35% (v / v) source water; water flow rate is 2.5 m / s) for 8 hours to gradually adapt to the environment and improve the survival rate; during the temporary raising, breadworms were fed, and the feeding amount was 5% of the total weight of the fish each time, 3 times a day;

[0052] (3) The filament schizothoracn fish after temporary raising recovered feeding and was transferred to a specialized raising pool (containing 15% (v / v) source water, water flow rate is 1 m / s) for intensive cultivation; the feed was breadworms, fish meal, chicken liver powder, fly larva powder, earthworm powder and maifanshi powder, and the mass ratio was 20:16:6:5:3:2; after mixing, the feed was fed, and the feeding amount was 7% of the total weight of the fish each time, 3 times a day;

[0053] (4) Fish disease prevention and control:

[0054] 1) Prevention and control of anchor head

[0055] When filament schizothoracn fish is found to have anchor head, 90% crystal M. c. is used to spray the whole pool, the drug dosage is 0.5 g / m 3 , and spraying is performed once every 7 days for 3 times, and 90% crystal M. c. is used for prophylactic spraying of the whole pool, the drug dosage is 0.5 g / m 3 , and spraying is performed once every 15 days;

[0056] 2) Prevention and control of fulminant septicemia

[0057] When filament schizothoracn fish is found to have fulminant septicemia, bleaching powder with a concentration of 20 g / m 3 is used for pond disinfection;

[0058] (5) Selection of parent fish, select parent fish with good physical condition and development, the selection standard is: after pressing the back of male fish, milky white semen flows out, which disperses in water, the female fish has red and swollen genital aperture, and the abdomen slightly shows the outline of ovary;

[0059] Select 3 female fish, 3 male fish, water temperature at 14.3℃, by intraperitoneal injection of oxytocin; each female fish injection: luteinizing hormone releasing hormone analog 8 μg + chorionic gonadotropin 250 IU + domperidone 5 μg, male fish half.

[0060] (6) After injection of oxytocin drugs, check the long filament split fish estrus and ovulation every 6 hours, long filament split fish parent fish injection oxytocin 86h after obvious estrus signs, timely artificial insemination, according to the ratio of ♀:♂ 1:1, using artificial dry insemination, a total of 2 female fish spawning, get eggs 13770;

[0061] (7) for postpartum parent fish after ovum or sperm collection, immediately enter the nursing state, the parent fish is respectively put into postpartum cultivation pool (containing 35% (v / v) of the source of water; water flow rate is 1 m / s) for postpartum cultivation, the bait for feeding is: fish meal, shrimp shell powder, yellow mealworm, black soldier fly larvae, fish premix, Amomum tsao-ko extract, radix astragali extract, radix stachys extract and sophora flavescens extract, mass ratio is 18:6:14:5:3:2:4:3:2, after mixing each component, feeding, feeding amount is: 6% of the total weight of fish each time, 3 times a day;

[0062] (8) for parent fish with body surface injury caused by artificial ovum or sperm collection, use erythromycin and yunnan white powder mixed repeatedly to smear the wound, for serious injury, especially due to extrusion injury and suspended in the water surface, can't swim normally, after its physical slightly recovered, each injection of 50,000 international units of penicillin;

[0063] For postpartum cultivation pool, every other day with 0.3g / m 3 Dibromine and 0.5g / m 3 of dimethoate (90%) alternately disinfect and insecticide; postpartum cultivation for 5d, then transfer to special breeding pool for further cultivation;

[0064] (9) using source of water, put the fertilized eggs into Yuxenko incubator for hatching, use air compressor to increase oxygen during hatching, the temperature of hatching is 18.3℃, the time is 15d, get long filament split fish fry;

[0065] (10) carry out initial cultivation for long filament split fish fry, the bait for feeding is: rotifer and nauplius of copepods, mass ratio is 1:3, feeding amount is 5% of the total weight of fish each time, 3 times a day, cultivate to body length >2cm, transfer to special breeding pool for further cultivation.

[0066] Example 2

[0067] A long filament split fish artificial breeding method, the steps are as follows:

[0068] (1) In March 2022, wild Schizothorax grahami were collected from the Longkai opening area of the Jinshajiang River, mainly using three-layer gill nets and traps. The collected wild Schizothorax grahami were disinfected with potassium permanganate, and those with severe injuries were treated with intramuscular injection of penicillin (10,000 IU / kg) 2 ml and erythromycin ointment. Jinshajiang River water was collected as the source water;

[0069] (2) The collected wild Schizothorax grahami were temporarily raised in an ecological simulation pool (containing 45% (v / v) source water; water flow rate 3.5 m / s) for 12 hours to gradually adapt to the environment and improve survival rate. Breadworms were fed during the temporary raising, with a feeding amount of 5% of the total weight of the fish each time, 3 times a day;

[0070] (3) After the temporary raising, the Schizothorax grahami resumed feeding and were transferred to a specialized raising pool (containing 20% (v / v) source water, water flow rate 2 m / s) for intensive cultivation. The feed was a mixture of breadworms, fish meal, chicken liver powder, fly larva powder, earthworm powder, and maifanite powder, with a mass ratio of 26:20:10:9:5:4. The mixture was fed, with a feeding amount of 7% of the total weight of the fish each time, 3 times a day;

[0071] (4) Fish disease prevention and control:

[0072] 1) Prevention and control of anchor head leeches

[0073] When anchor head leeches were found on Schizothorax grahami, 90% crystalline trichlorphon was used for whole pool spraying, with a dosage of 0.5 g / m 3 , spraying once every 7 days for 3 times. For prevention, 90% crystalline trichlorphon was used for whole pool spraying, with a dosage of 0.5 g / m 3 , spraying once every 15 days;

[0074] 2) Prevention and control of fulminant septicemia

[0075] When fulminant septicemia was found on Schizothorax grahami, chlorine dioxide disinfectant with a concentration of 10 g / m 3 was used for pond disinfection;

[0076] (5) Selection of parent fish, healthy and well-developed parent fish were selected, with the following selection criteria: after pressing the abdomen of male fish, white semen flows out, which disperses in water; the genital pore of female fish is red and swollen, and the abdomen slightly shows the outline of the ovary;

[0077] Three female fish and three male fish were selected, and the water temperature was 15.4°C. Oxytocin was injected into the abdominal cavity. Each female fish was injected with 12 μg of luteinizing hormone releasing hormone analogue, 350 IU of chorionic gonadotropin, and 7 μg of domperidone. The male fish were injected with half the amount.

[0078] (6) After injection of the spawning drug, the spawning and ovulation of the long filament brook loach are checked every 6 hours. After 90 hours of injection of the spawning drug, the parent loach shows obvious signs of spawning, and artificial insemination is performed in time. The ratio of ♀:♂ is 1:1, and artificial dry insemination is used. A total of 2 female loaches lay eggs, and 11057 eggs are obtained;

[0079] (7) The postpartum parent loach is immediately put into a postpartum cultivation pool after the completion of egg or sperm collection, and the parent loach is put into the postpartum cultivation pool (containing 45% (v / v) of the source water; the water flow rate is 2 m / s) for postpartum cultivation. The feed for feeding is fish meal, shrimp shell powder, yellow mealworm, black soldier fly larvae, fish premix, Amomum kravanh extract, Astragalus extract, Stemona extract, and Sophora flavescens extract, with a mass ratio of 24:10:16:9:5:4:8:5:4. After mixing the components, feeding is performed. The feeding amount is 6% of the total weight of the fish per feeding, and feeding is performed 3 times a day;

[0080] (8) For parent loaches with surface wounds caused by artificial egg or sperm collection, erythromycin and Yunnan white powder are repeatedly applied to the wounds. For seriously injured loaches, especially those suspended in the water surface and unable to swim normally due to squeezing, 50,000 international units of penicillin are injected per tail after their strength is slightly recovered;

[0081] For the postpartum cultivation pool, 0.3 g / m 3 of dichlorisohydline and 0.5 g / m 3 of trichlorphon (90%) are alternately disinfected and insecticidal; postpartum cultivation lasts for 9 days, and then the loaches are transferred to a special cultivation pool for further cultivation;

[0082] (9) The fertilized eggs are placed in a Yuxenko incubator and hatched using source water. Oxygen is increased using an air compressor during hatching. The hatching temperature is 21.1°C, and the hatching time is 19 days. The loach fry is obtained;

[0083] (10) The loach fry is initially cultivated by feeding rotifers and nauplii of copepods at a mass ratio of 3:1, with a feeding amount of 5% of the total weight of the fish per feeding, 3 times a day. The cultivation is continued until the body length is greater than 2 cm, and then the loach fry is transferred to a special cultivation pool for further cultivation.

[0084] Example 3

[0085] An artificial breeding method of the long filament brook loach, comprising the following steps:

[0086] (1) From March 2022 to the Longkai reservoir area of the Jinsha River, wild long filament brook loaches are captured using three-layer gill nets and traps. The captured wild long filament brook loaches are disinfected with povidone-iodine, and those with severe injuries are treated by intramuscular injection of penicillin (10,000 IU / kg) 2 ml and application of erythromycin ointment to the wounds. Jinsha River water is collected as source water;

[0087] (2) The captured wild gill rips fish in the ecological simulation pool (containing 40% (v / v) of the source water; water flow rate is 3 m / s) for 10 h, let them gradually adapt to the environment, improve the survival rate; feeding breadworms, feeding amount: 5% of the total weight of fish each time, 3 times a day;

[0088] (3) The gill rips fish after temporary nursing restore feeding, transfer to the specialized breeding pool (containing 18% (v / v) of the source water, water flow rate is 1.5 m / s) for intensive cultivation; feeding bait: breadworms, fish meal, chicken liver powder, fly larvae powder, earthworm powder and macrolite powder, mass ratio: 23:18:8:7:4:3, after mixing each component, feeding, feeding amount: 7% of the total weight of fish each time, 3 times a day;

[0089] (4) Fish disease prevention and control:

[0090] 1) Anchor head fly prevention

[0091] Found that gill rips fish have anchor head fly, use 90% crystal M. c. whole pool, use 0.5 g / m 3 , spray once every 7 days, spray for 3 times, prevent use 90% crystal M. c. whole pool, use 0.5 g / m 3 , spray once every 15 days;

[0092] 2) Prevention and control of fulminant septicemia

[0093] Found that gill rips fish have fulminant septicemia, use bleaching powder with a concentration of 20 g / m 3 to disinfect the pond;

[0094] (5) Selecting parent fish, selecting parent fish with good development and good physical condition, the selection standard is: after pressing the back of male fish, there is milky white semen flowing out, which disperses immediately when meeting water, the female fish has red and swollen genital aperture, and the abdomen slightly shows the outline of ovary;

[0095] Select 3 female fish and 3 male fish, the water temperature is 14.8℃, inject oxytocin through abdominal cavity; each female fish is injected with: luteinizing hormone releasing hormone analogue 10 μg + chorionic gonadotropin 300 IU + domperidone 6 μg, and the male fish is halved.

[0096] (6) After injecting the oxytocin drug, check the gill rips fish estrus and ovulation every 6 hours, the gill rips fish parent fish has obvious estrus signs 80 h after injecting the oxytocin drug, and artificial insemination is carried out in time, according to the ratio of ♀:♂ 1:1, using artificial dry insemination method, a total of 3 female fish spawn, and 21357 eggs are obtained;

[0097] (7) The post-spawning parents immediately enter the nursing state after the completion of ovum or sperm collection, and the parents are respectively put into the post-spawning cultivation pool (containing 40% (v / v) of the natural water source; the water flow speed is 1.5 m / s) for post-spawning cultivation, and the feed for feeding is: fish meal, shrimp shell powder, yellow mealworm, black soldier fly larvae, fish premix, Amomum kravanh extract, Astragalus extract, Stemona extract and Sophora flavescens extract, and the mass ratio is 21:8:15:7:4:3:6:4:3, and after mixing, the mixed components are fed, and the feeding amount is: 6% of the total weight of the fish each time, 3 times a day;

[0098] (8) For the parents with body surface injury caused by artificial ovum or sperm collection, erythromycin and Yunnan white powder are repeatedly applied to the wound, and for the seriously injured, especially those suspended in the water surface and cannot normally swim due to injury caused by extrusion, after their physical strength is slightly recovered, 50,000 international units of penicillin is injected into each tail;

[0099] For the post-spawning cultivation pool, 0.3 g / m 3 of dichlorisohydantoin and 0.5 g / m 3 of trichlorphon (90%) are alternately disinfected and insecticided every other day; the post-spawning cultivation lasts for 7 days, and then the parents are transferred into the special cultivation pool for further cultivation;

[0100] (9) The fertilized eggs are placed into the Yuxianke incubator for incubation by using the natural water source, and the air compressor is used for oxygenation during the incubation, the incubation temperature is 19.5℃, and the incubation time is 17 days, and the long filament schistura fish fry is obtained;

[0101] (10) The long filament schistura fish fry is subjected to initial cultivation, and the feed for feeding is: rotifer and nauplii of copepod, and the mass ratio is 1:1, and the feeding amount is: 5% of the total weight of the fish each time, 3 times a day, and the cultivation is carried out until the body length is greater than 2 cm, and then the fish fry is transferred into the special cultivation pool for further cultivation.

[0102] Comparative Example 1

[0103] The other methods are the same as those in Example 3, and the only difference is that in step (5), the oxytocin is replaced by: injection of the female fish: 2 mg of carp pituitary + 14 μg of chorionic gonadotropin + 1200 IU of luteinizing hormone-releasing hormone analogue; and the male fish is halved. Result: only 1 out of 3 female fish laid eggs, and 4835 eggs were obtained.

[0104] Comparative Example 2

[0105] The other methods are the same as those in Example 3, and the only difference is that in step (3), the feed is replaced by: breadworms and brine shrimp nauplii, and the mass ratio is 1:1. Result: 2 out of 3 female fish laid eggs, and 9685 eggs were obtained.

[0106] Comparative Example 3

[0107] Other methods are the same as example 3, the difference is only in the ecological simulation pool and the special pool do not contain the water of the biogenic source. Results: 3 female fish only 1 spawning, get 4643 eggs.

[0108] Experimental example 1

[0109] The number of fish eggs and fertilized eggs obtained in example 1 to example 3, comparative example 1 to comparative example 3 is calculated, the fertilization rate is calculated, after the fertilized eggs are hatched, the number of long filament schizothorax fry is counted, and the down pond rate is calculated, and the investigation results are shown in table 1.

[0110] Table 1 long filament schizothorax hatching results

[0111]

[0112] As shown in table 1, under the same conditions, the number of spawning parent fish in example 3 is the largest, the number of obtained eggs and the number of fertilized eggs are the largest, and the down pond rate is the highest, which shows that the artificial breeding environment created by example 3 method is more suitable for the growth of long filament schizothorax, and is more conducive to the hatching of long filament schizothorax.

[0113] From the data of comparative example 1 to comparative example 3, it can be seen that the selection of oxytocin is very important, which has a greater impact on the spawning of parent fish. The selection of bait will directly affect the growth state of parent fish, so it also has a greater impact on the spawning of parent fish. Whether to add the water of the biogenic source will lead to long filament schizothorax can not adapt to the new growth environment for a long time, and also lead to the poor growth state of fish, which finally affects the fecundity of parent fish.

[0114] Experimental example 2

[0115] The average weight and average total length of the down pond fry in example 1 to example 3, comparative example 1 to comparative example 3 are counted, and after 6 months of feeding, the average weight and average total length of the fry are counted again, and the results are shown in table 2.

[0116] Table 2 feed result investigation table

[0117]

[0118] From table 2, it can be seen that after 6 months of cultivation, the long filament schizothorax in example 3 group grows the fastest, which is significantly higher than that in other experimental groups, followed by example 1 group and example 2 group.

[0119] From the contents of table 1 and table 2, it can be seen that the growth environment and bait of fish have the greatest impact on the growth of long filament schizothorax. Only by finding the suitable breeding environment and bait can long filament schizothorax gradually adapt to the process of artificial breeding. From the data of comparative example 2 and comparative example 3, it can be seen that the improper setting of breeding environment and bait will significantly affect the growth of long filament schizothorax.

[0120] The above merely preferred embodiments of the present application, it should be noted that for those of ordinary skill in the art, without departing from the principles of the present application, can also make a number of improvements and refinements, these improvements and refinements should also be considered within the scope of the present application.

Claims

1. A method for artificial propagation of a longfin dace, characterized by, It comprises the following steps: (1) the wild caught long fin gill fish is first temporarily raised in an ecological simulation pool, and then transferred to a special breeding pool for intensive cultivation; (2) selecting parent fish and injecting oxytocin; (3) artificially inseminating the female fish in labor, and then putting the parent fish into postpartum cultivation pool for postpartum cultivation, hatching long fin gill fish fertilized eggs, and obtaining long fin gill fish fry; (4) primary cultivation of long fin gill fish fry, to body length > 2cm; The water flow speed in the ecological simulation pool of step (1) is 2.5~3.5m / s; The ecological simulation pool contains 35~45% of v / v: biogenic water; The water flow speed in the special breeding pool of step (1) is 1~2m / s; The special breeding pool contains 15~20% of v / v: biogenic water; The temporary cultivation time of step (1) is 8~12h; The feed for intensive cultivation is: breadworm, fish meal, chicken liver powder, fly larva powder, earthworm powder and maifanite powder, mass ratio: 20~26: 16~20: 6~10: 5~9: 3~5: 2~4; The oxytocin injection for each female fish of step (2) is: luteinizing hormone releasing hormone analogue 8~12μg + chorionic gonadotropin 250~350IU + domperidone 5~7μg, and the male fish is halved; The water flow speed in the postpartum cultivation pool of step (3) is 1~2m / s; The postpartum cultivation pool of step (3) contains 35~45% of v / v: biogenic water; The feed for postpartum cultivation of step (3) is: fish meal, shrimp shell powder, yellow mealworm, black soldier fly larvae, fish premix, Amomum kravanh extract, Astragalus extract, Stemona extract and Sophora flavescens extract, mass ratio 18~24: 6~10: 14~16: 5~9: 3~5: 2~4: 4~8: 3~5: 2~4; The hatching of step (3) uses biogenic water.

2. The method for artificial propagation of a longfin dace according to claim 1, characterized by, The hatching temperature of step (3) is 18.3~21.1℃, and the time is 15~19d.

3. The method for artificial propagation of a longfin dace according to claim 1, characterized by, The feed for primary cultivation of step (4) is: rotifer and copepod nauplii, mass ratio 1~3: 3~1.

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