A nanozyme with antibacterial function, its preparation method and application

Abstract

This invention belongs to the field of antibacterial technology, specifically relating to a nanozyme with antibacterial function, its preparation method, and its application. The nanozyme comprises an organic-inorganic composite material Fe-MOF@TP-TA-COF formed by encapsulating Fe-MOF with TP-TA-COF. CuS is loaded on the surface of the Fe-MOF@TP-TA composite material, forming Fe-MOF@TP-TA-COF@CuS. X Nanozymes, where X represents the weight percentage of CuS, X = 0.04, 0.08, 0.2; the Fe-MOF@TP-TA-COF@CuS x It exhibits near-infrared enhanced peroxidase-like activity.

Description

Technical Field

[0001] This invention belongs to the field of antibacterial technology, specifically relating to a nanozyme with antibacterial function, its preparation method, and its application. Background Technology

[0002] Bacterial infections remain a major threat to human health. The high mortality and morbidity rates caused by bacterial infections have drawn widespread global attention. Over the past few decades, the advent and widespread use of antibiotics have transformed medical treatment; however, the long-term misuse or uncontrolled use of antibiotics has led to increasing bacterial resistance, threatening public health through the food chain.

[0003] Nanozymes are nanomaterials with enzymatic properties. Compared to natural enzymes, nanozymes offer advantages such as simple preparation, stable properties, and low cost, and are widely used in biomedicine, industry, and environmental fields. In recent years, antibacterial methods based on nanozymes have received widespread attention and are considered a promising alternative strategy. Nanozyme antibacterial therapy typically utilizes peroxidase-like activity to decompose hydrogen peroxide (H₂O₂) into reactive oxygen species to kill bacteria. However, most nanozymes currently exhibit low catalytic activity under neutral conditions, which severely hinders their antibacterial efficacy. To develop more efficient antibacterial nanozymes, leveraging synergistic effects to enhance their antibacterial efficacy is crucial. Summary of the Invention

[0004] To address the problems existing in the prior art, this invention provides a nanozyme with antibacterial function, its preparation method, and its applications. The nanozyme of this invention exhibits excellent peroxidase-like activity and photothermal properties, and demonstrates good antibacterial effects against *Escherichia coli* and *Staphylococcus aureus*.

[0005] To achieve the above objectives, the present invention is implemented using the following technical solution:

[0006] A nanozyme with antibacterial function, comprising an organic-inorganic composite material Fe-MOF@TP-TA-COF formed by encapsulating Fe-MOF with TP-TA-COF, wherein CuS is loaded on the surface of the Fe-MOF@TP-TA composite material to form Fe-MOF@TP-TA-COF@CuS X Nanozymes, where X represents the weight percentage of CuS, X = 0.04, 0.08, 0.2; the Fe-MOF@TP-TA-COF@CuS x It exhibits near-infrared enhanced peroxidase-like activity.

[0007] This invention also provides a method for preparing nanozymes with antibacterial function, comprising the following steps:

[0008] (1) Fe-MOF was prepared by hydrothermal method;

[0009] (2) Fe-MOF@TP-TA-COF organic-inorganic composite material was synthesized by further hydrothermal method;

[0010] (3) CuS was loaded onto the surface of the Fe-MOF@TP-TA organic-inorganic composite material by in-situ sulfidation to form Fe-MOF@TP-TA-COF@CuS X Nanozymes.

[0011] More preferably, the specific operation of step (1) is as follows:

[0012] Aminoterephthalic acid (BDC-NH2) was dissolved in DMF, and then a mixture of FeCl3⋅6H2O and DMF was added. The mixture was stirred at room temperature for 1 hour, and then 2M NaOH solution was added to the mixture. The resulting mixture was transferred to a polytetrafluoroethylene autoclave and kept at 90-110℃ for 10-15 hours. After cooling to room temperature, the product was centrifuged, washed with DMF and methanol, and vacuum dried for 20-24 hours to obtain Fe-MOF.

[0013] Further preferably, the mass-to-volume ratio of BDC-NH2 to DMF is 0.81:15 (g / mL), and the mass-to-volume ratio of FeCl3⋅6H2O to DMF in the mixture of FeCl3⋅6H2O and DMF is 0.543:15 (g / mL).

[0014] Further preferably, the specific operation of step (2) is as follows: 1,3,5-tricarboxymethyl phloroglucinol (TP), 2,4,6-tris(4-aminophenyl)-1,3,5-triazine (TAPT) and Fe-MOF are added to a flask containing DMSO in a certain proportion. The solid mixture is heated and refluxed at a temperature of 120°C for 6 hours. The resulting solution is transferred to a polytetrafluoroethylene-lined reactor and heated in an oven at a temperature of 150°C for 36 hours. The mixture is washed three times with ethanol and dried under vacuum overnight to obtain Fe-MOF@TP-TA-COF.

[0015] According to a preferred embodiment of the present invention, in step (2), the mass ratio of TP, TAPT and Fe-MOF is 1:1.2:4.

[0016] Further preferably, the specific operation of step (3) is as follows: Fe-MOF@TP-TA-COF is added to deionized water and stirred to dissolve. Cu(NO3)2⋅6H2O and C2H5NS are added to the dispersion while stirring. After stirring at room temperature for 1 hour, the mixture is transferred to a high-pressure reactor lined with polytetrafluoroethylene and placed in an oven at 160°C for 5 hours. After natural cooling, the precipitate is collected by centrifugation. The precipitate is then washed three times with DMF and anhydrous ethanol, respectively. Finally, the collected product is dried in a vacuum drying oven at 60°C for 12 hours. This product is named Fe-MOF@TP-TA-COF@CuS X (X=0.04, 0.08, 0.2), where X represents the weight percentage of CuS.

[0017] More preferably, in step (3), the mass ratio of Cu(NO3)2⋅6H2O and C2H5NS is 4:1.

[0018] More preferably, in step (3), the ratio of Fe-MOF@TP-TA-COF to deionized water is 0.1g:25mL.

[0019] The present invention also provides the application of the nanozyme in the elimination of Staphylococcus aureus and Escherichia coli. The nanozyme exhibits the best peroxidase-like performance at pH 4 and the strongest catalytic activity at a temperature of 50°C.

[0020] According to a preferred embodiment of the present invention, the specific antibacterial method of the nanozyme is as follows:

[0021] 1) Before the experiment, pipette tips, centrifuge tubes, and other equipment used in the experiment should be sterilized at 120°C in an autoclave. Dissolve 10-15g of trypsin, 5-10g of yeast powder, and 10-15g of NaCl in 1L of ultrapure water to obtain Luria-Bertani (LB) liquid culture medium. Add 18-20g of agar to the liquid culture medium to obtain a solid culture medium. After preparation, sterilize the medium in an autoclave. Add the bacterial culture to the LB culture medium and place it in a shaker at a constant temperature of 37°C and a shaking speed of 140rpm for 8-12 hours. Bacterial cells were collected by centrifugation at 5000 rpm for 3 minutes, followed by washing three times with PBS buffer (pH 7, 10 mM). The resulting bacteria were then dispersed in the buffer at a bacterial-to-buffer volume ratio of 1:250. The bacterial concentration was measured using a UV-Vis spectrophotometer at 600 nm. The absorbance at 600 nm was 0.1, indicating a bacterial concentration of ~10-10. 8 CFU / mL;

[0022] 2) To investigate the in vitro antibacterial function of Fe-MOF@TP-TA-COF@CuS, bacteria were divided into the following 8 groups and placed in well plates: (1) bacteria; (2) bacteria + near-infrared; (3) bacteria + H2O2; (4) bacteria + near-infrared + H2O2; (5) bacteria + Fe-MOF@TP-TA-COF@CuS; (6) bacteria + Fe-MOF@TP-TA-COF@CuS + H2O2; (7) bacteria + Fe-MOF@TP-TA-COF@CuS + near-infrared; (8) bacteria + Fe-MOF@TP-TA-COF@CuS + near-infrared + H2O2, and the concentration of the bacteria was 1×10⁻⁶. 6 / CFUmL -1 The concentration of H2O2 was 100 µM; the concentration of Fe-MOF@TP-TA-COF@CuS was 30 µg / mL; groups (2), (4), (7), and (8) were further exposed to near-infrared (NIR) laser light with a wavelength of 808 nm and a power of 1.5 W / cm². 2 The irradiation time was 5 minutes; the steps after NIR irradiation were the same as those for groups (1), (3), (5), and (6). The total volume of the solution in each well was 0.2 mL. After incubation for 4 hours, 100 µL of bacterial suspension from groups (1) to (8) was spread on an agar plate and incubated at 37°C for 12 hours, and then the colonies were counted. All experiments were repeated three times.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0024] 1. The Fe-MOF@TP-TA-COF@CuS composite nanomaterial prepared in this invention exhibits excellent peroxidase-like activity and photothermal properties, and demonstrates enhanced near-infrared peroxidase-like activity. Therefore, under near-infrared irradiation, it can release a large amount of reactive oxygen species through strong peroxidase-like activity, and generate heat through the material's excellent photothermal properties, thereby achieving bacterial elimination through a synergistic effect.

[0025] 2. The composite nanozyme has a significant disinfecting effect on Escherichia coli and Staphylococcus aureus.

[0026] 3. The composite nanozyme has good biocompatibility. Attached Figure Description

[0027] Figure 1 TEM image of the Fe-MOF nanozyme prepared in Example 1;

[0028] Figure 2 TEM image of the Fe-MOF@TP-TA-COF nanozyme prepared in Example 1;

[0029] Figure 3TEM image of the Fe-MOF@TP-TA-COF@CuS nanozyme prepared in Example 1;

[0030] Figure 4 The XRD patterns of Fe-MOF, Fe-MOF@TP-TA-COF, and Fe-MOF@TP-TA-COF@CuS prepared in Example 1 are shown below.

[0031] Figure 5 The photothermally enhanced peroxidase-like activity of Fe-MOF@TP-TA@CuS obtained in step (1) of Example 1.

[0032] Figure 6 Colony diagrams of Escherichia coli and Staphylococcus aureus under different conditions. Detailed Implementation

[0033] To better understand the above-mentioned objectives, features, and advantages of the present invention, the present invention will be further described below with reference to specific embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0034] Unless otherwise specified, the experimental methods described in the following examples are conventional methods.

[0035] The photothermal conversion efficiency of the composite nanozyme is calculated using the following formula:

[0036] η=[ℎS(T max -T surr )]−Q dis / [I(1-10 A 808 )](Formula 1)

[0037] Where h is the heat transfer coefficient, S is the container surface area, and T is the heat transfer coefficient. max T is the highest steady-state temperature of the sample solution. surr Q represents the ambient temperature. dis I represents the heat input based on the absorption of light by the solvent and container, and A represents the laser power. 808 The absorbance of the sample solution is 808 nm. Unless otherwise specified, all reagents and materials used in the following examples are commercially available.

[0038] Example 1

[0039] Synthesis of nanozyme Fe-MOF@TP-TA-COF@CuS:

[0040] 1) Weigh 2g of NaOH and add it to an appropriate amount of deionized water. Stir to dissolve it, transfer the solution to a 50ml volumetric flask and make up to volume to prepare a 2M NaOH solution. Shake well and set aside.

[0041] 2) Weigh 1.62g of FeCl3⋅6H2O and add it to 30mL of DMF. Dissolve the solution at room temperature and label this as solution 1. Add 1.086g of BDC-NH2 to 30mL of DMF and stir until dissolved at room temperature. Label this as solution 2. Add solution 2 to solution 1 to form a homogeneous solution. Add 2.4mL of 2M NaOH solution dropwise to the homogeneous solution and continue stirring at room temperature for one hour. Then transfer the above solution to a polytetrafluoroethylene-lined reactor. Place the polytetrafluoroethylene-lined reactor in an oven and solvothermal react at 100℃ for 12 hours. After cooling to room temperature, collect the brown solid by centrifugation. Wash twice with DMF, then twice with methanol, and wash with water until the supernatant is colorless. Then transfer the supernatant to 400mL of ultrapure water and stir overnight to remove excess DMF solvent molecules. Collect the solid by centrifugation, and wash the product twice with an appropriate amount of methanol. Then dry the product in a vacuum drying oven at 60℃ for 12 hours to obtain Fe-MOF.

[0042] 3) Fe-MOF@TP-TA was synthesized using a further hydrothermal method. 0.042 g TP, 0.0504 g TAPT, and 0.168 g Fe-MOF were added to a 25 mL flask containing 5 mL DMSO. The solid mixture was heated to 120 °C and refluxed for 6 h. The resulting solution was transferred to a polytetrafluoroethylene-lined reactor and heated in an oven at 150 °C for 36 h. The mixture was washed three times with ethanol and dried under vacuum overnight to obtain Fe-MOF@TP-TA-COF powder.

[0043] 4) Fe-MOF@TP-TA-COF@CuS was synthesized using the original sulfidation method. 0.1 g of Fe-MOF@TP-TA-COF was added to 25 mL of deionized water and stirred until dissolved. While stirring, different masses of Cu(NO3)2⋅6H2O and C2H5NS were added to the dispersion, with a mass ratio of Cu(NO3)2⋅6H2O to C2H5NS of 4:1. After stirring at room temperature for 1 h, the mixture was transferred to a polytetrafluoroethylene-lined high-pressure reactor and reacted in a 160℃ oven for 5 h. After natural cooling, the precipitate was collected by centrifugation. The precipitate was then washed three times with DMF and anhydrous ethanol, respectively. Finally, the collected product was dried in a vacuum drying oven at 60℃ for 12 h. This product was named Fe-MOF@TP-TA@CuS. X (X=0.04, 0.08, 0.2), where X represents the weight percentage of CuS.

[0044] The material synthesized in Example 1 was characterized. Figure 1 This is a transmission electron microscope image of the Fe-MOF nanozyme prepared in Example 1. Figure 2 This is a transmission electron microscope image of the Fe-MOF@TP-TA nanozyme prepared in Example 1; Figure 3 The image shows a transmission electron microscope (TEM) image of the Fe-MOF@TP-TA-COF@CuS nanozyme prepared in Example 1. Figure 4 The XRD patterns are of Fe-MOF, Fe-MOF@TP-TA, and Fe-MOF@TP-TA-COF@CuS prepared in Example 1.

[0045] Experiment Example 2

[0046] Fe-MOF@TP-TA-COF@CuS x Peroxidase-like performance evaluation

[0047] The Fe-MOF@TP-TA-COF@CuSx prepared in Example 1 was dispersed in water to prepare a dispersion. The dispersion (200 μL, 0.1 mg / mL) was added to a NaAc / HAc buffer (1.2 mL) with a pH of 2-11. Then, H2O2 (200 μL, 10 mM) and TMB (200 μL, 0.3 mM) were added. The mixture was reacted at room temperature for 5 min. The absorbance of the mixed solution was measured at 652 nm using a UV-Vis spectrophotometer to obtain the relative activities of peroxidases of different materials, as shown in Table 1.

[0048] Table 1. Relative activities of Fe-MOF@TP-TA@CuSx peroxidases loaded with different weight percentages of CuS.

[0049]

[0050] As shown in Table 1, the material exhibits the strongest peroxidase-like activity when the weight percentage of CuS loading is 0.08%. Therefore, Fe-MOF@TP-TA@CuS was selected. 0.08 For use in subsequent research.

[0051] Experimental Example 3

[0052] Fe-MOF@TP-TA@CuS at different pH levels 0.08 Peroxidase-like performance evaluation

[0053] The Fe-MOF@TP-TA@CuS prepared in Example 1 was used. 0.08 The mixture was dispersed in water to prepare a dispersion. The dispersion (200 µL, 0.1 mg / mL) was added to a NaAc / HAc buffer (1.2 mL) with a pH of 2–11. Then, H2O2 (200 µL, 10 mM) and TMB (200 µL, 0.3 mM) were added. The mixture was reacted at room temperature for 5 min. The absorbance of the mixed solution was measured at 652 nm using a UV-Vis spectrophotometer. The relative activities of the peroxidase-like enzymes at different pH values ​​were obtained, as shown in Table 1.

[0054] Table 2 Fe-MOF@TP-TA@CuS at different pH values 0.08 The relative activity of peroxidase-like enzymes.

[0055]

[0056] Most nanozymes exhibit optimal catalytic activity under acidic conditions, with almost no catalytic activity in neutral or alkaline environments. This composite material, however, still exhibits high catalytic activity under neutral conditions. As shown in Table 2, the peroxidase-like performance is optimal at pH 4, and it still maintains high catalytic activity in the bacterial survival environment (pH 7), providing favorable conditions for antibacterial activity.

[0057] Experiment Example 4

[0058] Fe-MOF@TP-TA-COF@CuS at different temperatures 0.08 Peroxidase-like performance evaluation

[0059] Fe-MOF@TP-TA-COF@CuS prepared in Example 1 0.08 The mixture was dispersed in water to prepare a dispersion. The dispersion (200 μL, 0.1 mg / mL) was added to a NaAc / HAc buffer (1.2 mL) at pH 4. Then, H2O2 (200 μL, 10 mM) and TMB (200 μL, 0.3 mM) were added. The mixture was reacted at different temperatures for 5 min. The absorbance of the mixed solution was measured at 652 nm using a UV-Vis spectrophotometer. The relative activities of the peroxidase-like enzymes at different temperatures were obtained, as shown in Table 3.

[0060] Table 3. Evaluation of peroxidase-like activity of Fe-MOF@TP-TA@CuS0.08 at different temperatures.

[0061]

[0062] The catalytic activity of nanozymes is greatly affected by temperature, and this nanozyme exhibits the strongest catalytic activity at 50℃.

[0063] Experimental Example 5

[0064] Fe-MOF@TP-TA@CuS 0.08 Photothermal properties

[0065] To study Fe-MOF@TP-TA@CuS 0.08 Photothermal properties of Fe-MOF@TP-TA-COF@CuS 0.08Dispersed in deionized water and diluted to different concentrations (0-100 µg / mL), and subjected to different power densities (0-2.0 W / cm²). 2 The photothermal performance of the sample was investigated by irradiating it with an 808nm laser for 10 minutes. The photothermal conversion efficiency was calculated based on Equation 1, and found to be 48.7%.

[0066] Experimental Example 6

[0067] Fe-MOF@TP-TA-COF@CuS 0.08 Near-infrared enhanced peroxidase-like activity

[0068] Evaluation of Fe-MOF@TP-TA-COF@CuS using TMB oxidation assay 0.08 Near-infrared light enhances the peroxidase-like activity, oxidizing TMB to oxidized TMB (oxTMB), which exhibits a unique absorption peak at 652 nm. Interestingly, when the colorimetric system is exposed to near-infrared light, its absorbance significantly increases compared to the control group. Fe-MOF@TP-TA-COF@CuS 0.08 Peroxidase-like activity was significantly increased. Figure 5 Therefore, the results show that Fe-MOF@TP-TA-COF@CuS 0.08 It exhibits near-infrared enhanced peroxidase-like activity.

[0069] Experimental Example 7

[0070] Fe-MOF@TP-TA@CuS 0.08 In vitro antibacterial test

[0071] The Fe-MOF@TP-TA-COF@CuS strain was investigated using Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. 0.08 To assess the in vitro antibacterial function, bacteria were divided into the following 8 groups and placed in well plates: (1) PBS + bacteria; (2) PBS + bacteria + NIR; (3) PBS + bacteria + H2O2; (4) PBS + bacteria + NIR + H2O2; (5) PBS + bacteria + Fe-MOF@TP-TA@CuS 0.08 (6) PBS + bacteria + Fe-MOF@TP-TA@CuS 0.08 + H2O2;(7)PBS+bacteria+Fe-MOF@TP-TA@CuS 0.08 +NIR;(8)PBS+bacteria+Fe-MOF@TP-TA@CuS 0.08 +NIR+ H2O2. Groups (2), (4), (7), and (8) were further exposed to NIR laser (808 nm, 1.5 W / cm²). 25 minutes. The procedure after NIR irradiation was the same as for groups (1), (3), (5), and (6). Fe-MOF@TP-TA@CuS 0.08 The final concentrations of H2O2 and bacteria were 30 µg / mL, 100 µM, and 1 × 10⁶ CFU / mL, respectively. The total volume of solution in each well was 0.2 mL. After incubation for 4 hours, 100 µL of bacterial suspension from groups (1)-(8) was spread onto an agar plate and incubated at 37 °C for 12 hours, after which colonies were counted. All experiments were performed in triplicate. Figure 6 It can be seen that, regardless of whether near-infrared light irradiation is provided, PBS buffer and H2O2, as well as Fe-MOF@TP-TA@CuS alone, 0.08 None of the groups showed antibacterial activity, and the corresponding LB agar plates were completely covered by bacterial colonies. PBS + bacteria + Fe-MOF@TP-TA@CuS 0.08 The bacterial colonies in the + H2O2 group decreased significantly after near-infrared light irradiation, but some colonies still grew even without near-infrared light irradiation. Under near-infrared light irradiation, Fe-MOF@TP-TA@CuS... 0.08 +H₂O₂ showed the highest antibacterial activity, with no colony formation observed on LB agar plates. Bacterial viability was determined using the plate count method. Fe-MOF@TP-TA@CuS 0.08 The +H2O2 group has considerable bacterial clearance ability, with a clearance rate of >99.999% for Staphylococcus aureus and Escherichia coli.

[0072] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A nanozyme with antibacterial function, characterized in that: The nanozyme comprises an organic-inorganic composite material Fe-MOF@TP-TA-COF formed by encapsulating Fe-MOF with TP-TA-COF. CuS is loaded on the surface of the Fe-MOF@TP-TA composite material to form Fe-MOF@TP-TA-COF@CuS. X Nanozymes, where X represents the weight percentage of CuS, X = 0.04, 0.08, or 0.2; The Fe-MOF@TP-TA-COF@CuS x It exhibits near-infrared enhanced peroxidase-like activity; The preparation method of the nanozyme with antibacterial function includes the following steps: (1) Preparation of Fe-MOF; (2) Synthesis of Fe-MOF@TP-TA-COF organic-inorganic composite material: 1,3,5-tricarboxymethyl phloroglucinol TP, 2,4,6-tris(4-aminophenyl)-1,3,5-triazine TAPT and Fe-MOF were added to a flask containing DMSO in a certain proportion. The solid mixture was heated and refluxed at 120°C for 6 hours. The resulting solution was transferred to a polytetrafluoroethylene-lined reactor and heated in an oven at 150°C for 36 hours. The mixture was washed three times with ethanol and dried under vacuum overnight to obtain Fe-MOF@TP-TA-COF. (3) In-situ sulfidation method is used to load CuS onto the surface of Fe-MOF@TP-TA organic-inorganic composite material to form Fe-MOF@TP-TA-COF@CuS X Nanozymes.

2. The nanoenzyme with antibacterial function according to claim 1, characterized in that: The nanozyme exhibits optimal peroxidase-like performance at pH 4 and strongest catalytic activity at 50°C.

3. A method for preparing a nanozyme with antibacterial function as described in any one of claims 1-2, characterized in that: The steps include the following: (1) Preparation of Fe-MOF; (2) Fe-MOF@TP-TA-COF organic-inorganic composite material was synthesized by further hydrothermal method; (3) In-situ sulfidation method is used to load CuS onto the surface of Fe-MOF@TP-TA organic-inorganic composite material to form Fe-MOF@TP-TA-COF@CuS X Nanozymes.

4. The method for preparing nanozymes with antibacterial function according to claim 3, characterized in that: The specific operation of step (1) is as follows: Aminoterephthalic acid BDC-NH2 was dissolved in DMF, and then a mixture of FeCl3⋅6H2O and DMF was added. The mixture was stirred at room temperature for 1 hour, and then 2M NaOH solution was added to the mixture. The resulting mixture was transferred to a polytetrafluoroethylene autoclave and kept at 90-110℃ for 10-15 hours. After cooling to room temperature, the product was centrifuged, washed with DMF and methanol, and vacuum dried for 20-24 hours to obtain Fe-MOF.

5. The method for preparing nanozymes with antibacterial function according to claim 4, characterized in that: The mass-to-volume ratio of BDC-NH2 to DMF is 0.81:15 (g / mL), and the mass-to-volume ratio of FeCl3⋅6H2O to DMF in the mixture of FeCl3⋅6H2O and DMF is 0.543:15 (g / mL).

6. The method for preparing nanozymes with antibacterial function according to claim 3, characterized in that: The specific operation of step (2) is as follows: 1,3,5-tricarboxymethyl phloroglucinol (TP), 2,4,6-tris(4-aminophenyl)-1,3,5-triazine (TAPT), and Fe-MOF were added to a flask containing DMSO in a certain proportion. The solid mixture was heated and refluxed at 120°C for 6 hours. The resulting solution was transferred to a polytetrafluoroethylene-lined reactor and heated in an oven at 150°C for 36 hours. The mixture was washed three times with ethanol and dried under vacuum overnight to obtain Fe-MOF@TP-TA-COF.

7. The method for preparing nanozymes with antibacterial function according to claim 6, characterized in that: The mass ratio of TP, TAPT and Fe-MOF is 1:1.2:

4.

8. The method for preparing nanozymes with antibacterial function according to claim 3, characterized in that: The specific operation of step (3) is as follows: Fe-MOF@TP-TA-COF is added to deionized water and stirred to dissolve. Cu(NO3)2⋅6H2O and C2H5NS are added to the dispersion while stirring. After stirring at room temperature for 1 hour, the mixture is transferred to a high-pressure reactor lined with polytetrafluoroethylene and placed in an oven at 160°C for 5 hours. After natural cooling, the precipitate is collected by centrifugation. The precipitate is then washed three times with DMF and anhydrous ethanol, respectively. Finally, the collected product is dried in a vacuum drying oven at 60°C for 12 hours. The product is named Fe-MOF@TP-TA-COF@CuS X X = 0.04, 0.08, or 0.2, where X represents the weight percentage of CuS.

9. The method for preparing nanozymes with antibacterial function according to claim 8, characterized in that: In step (3), the mass ratio of Cu(NO3)2⋅6H2O and C2H5NS is 4:1; The ratio of Fe-MOF@TP-TA-COF to deionized water is 0.1g:25mL.

10. The use of a nanozyme with antibacterial function as described in any one of claims 1-2, or a nanozyme prepared by any one of the preparation methods in claims 3-7, in the preparation of drugs for disinfecting Staphylococcus aureus and Escherichia coli.

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