A method for promoting production of phenoxazine-1-carboxylic acid by limiting a competing pathway for phenoxazine synthesis

By using genetic engineering to restrict the siderophore and salicylic acid synthesis pathways of *Pseudomonas aeruginosa*, engineered strains QPCA-8 and QPCA-9 were constructed. This solved the problem of low efficiency in the production of phenazine-1-carboxylic acid by *Pseudomonas aeruginosa*, significantly increasing the yield of phenazine-1-carboxylic acid and reducing production costs.

CN117925493BActive Publication Date: 2026-06-26QILU UNIVERSITY OF TECHNOLOGY (SHANDONG ACADEMY OF SCIENCES)
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Patent Information

Application Number
CN202410174661.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-02-07
Publication Date
2026-06-26
Estimated Expiration
2044-02-07

AI Technical Summary

Technical Problem

The current efficiency of Pseudomonas aeruginosa in producing phenazine-1-carboxylic acid is low, resulting in high production costs and limiting its widespread application in biopesticides.

Method used

By restricting the siderophore and salicylic acid synthesis pathway of *Pseudomonas aeruginosa*, and by using genetic engineering to knock out the ycaC and/or pabC genes, engineered strains QPCA-8 and QPCA-9 were constructed. This guided more carbon flux into the phenazine synthesis pathway, thereby improving the production efficiency of phenazine-1-carboxylic acid.

Benefits of technology

The production efficiency of phenazine-1-carboxylic acid was significantly improved, with the yield of strain QPCA-8 increasing to 8914 mg/L and the yield of QPCA-9 further increasing to 9948 mg/L, while reducing production costs.

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Abstract

The application belongs to the technical field of genetic engineering, and provides a method for promoting production of phenoxazine-1-carboxylic acid by limiting competition pathways of phenoxazine synthesis. The application takes high-yield strain QPCA-7 as a starting strain, limits the synthesis pathways of salicylic acid, siderophore and the like of phenoxazine, and realizes the improvement of the yield of phenoxazine-1-carboxylic acid of the strain. ycaC The application obtains an engineering strain by non-scarring knockout of the gene for limiting the synthesis of siderophore in Pseudomonas, and the strain is named QPCA-8, and the yield of phenoxazine-1-carboxylic acid of the strain is improved. pabC The application knocks out the gene for limiting the production of salicylic acid in Pseudomonas on the basis of QPCA-8, and the yield of phenoxazine-1-carboxylic acid of the strain is improved.
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