Traditional Chinese medicine composition, medicinal solution, preparation method and application for treating ulcerative colitis

Abstract

The invention discloses a Chinese medicine composition for treating ulcerative colitis, a medicine liquid, a preparation method and an application thereof, and belongs to the technical field of Chinese medicine prescriptions. The invention solves the problem that the existing treatment of UC is mainly based on aminosalicylic acid drugs, glucocorticoids and immunosuppressants. The advantages of these drugs are short-term effectiveness and rapid symptom relief, but there are problems of recurrence of the disease after drug withdrawal and large side effects of long-term medication. The Chinese medicine composition is composed of the following raw materials: 8-12 parts of indigo naturalis; 25-32 parts of red peony root; 18-23 parts of phellodendron amurense; 15-22 parts of alum; 28-31 parts of ebony root; 8-10 parts of bletilla striata; 8-10 parts of eclipta prostrata; and 12-15 parts of poria cocos, which can regulate water and blood and greatly facilitate the recovery of ulcerative colitis. Both bletilla striata and poria cocos have dispersed colloid, which can cover the ulcer during enema to form a medicine film and improve the cure rate.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine composition, a medicinal liquid, a preparation method and an application thereof for treating ulcerative colitis. Background Art

[0002] Ulcerative colitis (UC) is a chronic, immune-mediated inflammatory disease that often affects the rectum and sigmoid colon. Abdominal pain, diarrhea, bloody stools, and weight loss are its main symptoms. UC is characterized by a long course of disease, repeated attacks, and difficulty in healing. The current treatment of UC in clinical medicine is mainly aminosalicylic acid drugs, glucocorticoids, and immunosuppressants. The advantages of these drugs are that they take effect in a short time and relieve symptoms quickly, but they have disadvantages such as recurrence of the disease after discontinuation of the drug and large side effects of long-term medication. Traditional Chinese medicine does not have a disease name for UC, but according to the clinical manifestations of the disease, it should be classified as "intestinal diarrhea" and "diarrhea" in traditional Chinese medicine. In terms of etiology, it is believed that the disease is mostly caused by the six exogenous evil spirits, which cause damp-heat and blood stasis to invade the large intestine, and involve many internal organs, such as the spleen, kidney, liver, and lung. Summary of the invention

[0003] The technical solution adopted by the present invention is as follows:

[0004] The Chinese medicine composition for treating ulcerative colitis is composed of the following raw materials by weight:

[0005] 8-12 parts of Indigo; 25-32 parts of Red Peony Root; 18-23 parts of Phellodendron Amurense; 15-22 parts of Alum; 28-31 parts of Prunus Mume; 8-10 parts of Bletilla striata; 8-10 parts of Eclipta prostrata; 12-15 parts of Poria.

[0006] Preferably, the drug is composed of the following raw materials by weight: 12 parts of Indigo Naturalis; 32 parts of Red Peony Root; 23 parts of Phellodendron Amurense; 22 parts of Alum; 31 parts of Prunus Mume; 10 parts of Bletilla striata; 10 parts of Eclipta Prostrata; and 15 parts of Poria.

[0007] The Chinese medicinal liquid for treating ulcerative colitis comprises the above-mentioned Chinese medicinal composition for treating ulcerative colitis.

[0008] A method for preparing a Chinese medicinal liquid for treating ulcerative colitis comprises the following steps:

[0009] Step 1: Wash and crush the Chinese medicine composition for treating ulcerative colitis, and add water to submerge the medicine;

[0010] Step 2: Soak for 30 to 40 minutes, heat to boiling, maintain boiling for 60 to 80 minutes, filter, and obtain the first filter residue and the first filtrate;

[0011] Step 3: Add water to submerge the first filter residue, heat to boiling, maintain boiling for 40 to 60 minutes, filter, and obtain the second filter residue and the second filtrate;

[0012] Step 4: Evenly mix the first filtrate and the second filtrate, and concentrate by boiling to 1 / 3 to 1 / 3.5 of the original volume to obtain a Chinese medicine solution for treating ulcerative colitis.

[0013] The application of the Chinese medicine composition for treating ulcerative colitis is to administer the medicine orally or by enema to treat the disease.

[0014] Preferably, it is used to treat ulcerative colitis by oral administration or enema.

[0015] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0016] Although ulcerative colitis is located in the intestines, it is related to all five internal organs. The lungs and large intestine are exterior and interior to each other, and the lung qi is responsible for descending, regulating the water channels, distributing body fluids, and helping the large intestine to conduct downward. The spleen is responsible for muscles and is the foundation of acquired constitution. The intestines are also part of the muscles of the whole body, and their physiological functions naturally rely on the healthy function of the spleen and stomach. The kidneys are the water organs, the large intestine is responsible for body fluids, and the small intestine is responsible for fluids. If the kidney yang is weak, water and moisture will not be transformed and will move downward. If the kidney is weak and the opening and closing are not functioning properly, the stomach will not be solid, which is the so-called "the heat in the cauldron is in the firewood on the stove, and the root of spleen yang is in the gate of life". On the contrary, ulcerative colitis has a long course and often recurs. If it is prolonged, it will inevitably damage kidney yang, which will inevitably lead to the decline of fire in the gate of life. The liver is responsible for the release of qi, Regulate the whole body's Qi. Anger, depression, and emotional frustration will cause the liver to lose its ability to vent, which will hinder the large intestine from descending, and easily cause the dregs of Qi to combine with dampness, heat, and stasis. The heart and small intestine are exterior and interior to each other, and the small intestine has a direct function of separating the clear from the turbid, which affects the large intestine. Therefore, the five internal organs can all affect UC, just as "the five internal organs all cause diarrhea, not just the intestines." Therefore, intestinal diseases are treated by the five internal organs (that is, the five internal organs treat the intestines), so the five colors corresponding to the five internal organs are selected: blue, red, yellow, white, and black, which correspond to the liver, heart, spleen, lung, and kidney. Further, five drugs, indigo, red peony root, phellodendron, alum, and black plum, are selected to act on the five internal organs for comprehensive treatment.

[0017] Indigo is salty and cold in nature. It mainly enters the liver meridian. It mainly clears away heat and detoxifies, cools blood and eliminates spots, and relieves fire and calms nerves.

[0018] Red peony root, smoothes blood vessels, relieves pain, disperses bad blood, removes evil blood, removes water qi, and benefits the bladder and large and small intestines;

[0019] Huangbai, Huangbai is bitter and cold, dry and sinking, enters the kidney and bladder meridians, clears away real heat (fire) and relieves heat toxicity, clears kidney fire and relieves false heat, is an important medicine for treating damp-heat and fire toxicity, and is widely used in damp-heat and fire toxicity diseases;

[0020] Alum is made from the sulfate mineral alunite through processing and refining. It tastes sour and astringent, is cold in nature, and is suitable for the lungs, spleen, liver, and large intestine. It has the effects of detoxification and insecticide, drying dampness and relieving itching, stopping bleeding and diarrhea, and removing wind and phlegm.

[0021] Black plum is sour, warm and astringent, removes phlegm, treats malaria and miasma, astringes the lungs and astringes the intestines, relieves chronic cough and diarrhea;

[0022] Bletilla striata generally refers to Bletilla striata, which is slightly cold in nature and belongs to the lung meridian and stomach meridian. It has the effect of astringing and stopping bleeding, and can relieve symptoms of lung and stomach bleeding such as coughing up blood, vomiting blood, and hemoptysis.

[0023] Eclipta prostrata enters the liver and kidney meridians, can nourish the yin of the liver and kidneys, and treat liver and kidney yin deficiency.

[0024] Poria cocos has the effects of promoting diuresis and eliminating dampness, strengthening the spleen, and calming the mind;

[0025] Indigo, red peony root, phellodendron, alum and black plum correspond to the five internal organs, and the five internal organs are treated together, and they influence each other. Bletilla striata and Eclipta prostrata cooperate with the previous five medicines to have the effects of tonifying deficiency, benefiting the spleen, astringing and stopping bleeding, and can effectively treat intestinal ulcers.

[0026] Poria cocos is neutral in nature. When used with red peony root, it can alleviate the coldness of Indigo, red peony root, Phellodendron, and Alum. The combination of Poria cocos and red peony root can regulate water and blood, which is very beneficial to the recovery of ulcerative colitis.

[0027] Both Bletilla striata and Poria cocos contain dispersible colloids, which can cover the ulcer during enema to form a drug film and improve the cure rate. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

[0029] Example 1

[0030] The following steps are used to prepare a Chinese medicinal liquid for treating ulcerative colitis:

[0031] Step 1: Wash and crush the following Chinese medicine composition: Indigo naturalis 8g; Red peony root 25g; Phellodendron amurense 18g; Alum 15g; Prunus mume 28g; Bletilla striata 8g; Eclipta prostrata 8g; Poria 12g, add water to submerge the medicines;

[0032] Step 2: Soak for 35 minutes, heat to boiling, maintain boiling for 70 minutes, filter, and obtain the first filter residue and the first filtrate;

[0033] Step 3: Add water to submerge the first filter residue, heat to boiling, maintain boiling for 50 minutes, filter, and obtain the second filter residue and the second filtrate;

[0034] Step 4: Evenly mix the first filtrate and the second filtrate, boil and concentrate them to 1 / 3 of the original volume to obtain a Chinese medicine solution for treating ulcerative colitis.

[0035] Example 2

[0036] Different from Example 1, in this example, the Chinese medicine composition consists of 10g of Indigo Naturalis, 28g of Red Peony Root, 20g of Phellodendron Amurense, 17g of Alum, 30g of Prunus Mume, 9g of Bletilla striata, 9g of Eclipta Prostrata, and 13g of Poria.

[0037] Example 3

[0038] Different from Example 1, in this example, the Chinese medicine composition consists of 12g of Indigo Naturalis, 32g of Red Peony Root, 23g of Phellodendron Amurense, 22g of Alum, 31g of Prunus Mume, 10g of Bletilla striata, 10g of Eclipta Prostrata, and 15g of Poria.

[0039] Example 4

[0040] Different from Example 1, in this example, the Chinese medicine composition comprises 9g of Indigo Naturalis, 28g of Red Peony Root, 23g of Phellodendron Amurense,

[0041] Alum 20g; Prunus mume 31g; Bletilla striata 10g; Eclipta prostrata 10g; Poria cocos 15g.

[0042] Comparative Example 1

[0043] Different from Example 1, in this example, the Chinese medicine composition consists of 100g Sophora flavescens, 100g Sanguisorba officinalis charcoal, 67g Bletilla striata, 33g Indigo naturalis, 50g Licorice, 16.5g Panax notoginseng, 50g Agrimoniae, 100g Coix lachryma-jobi, 67g Phellodendron chinense, 67g Coptis chinensis, 67g Qinpi, and 100g Pulsatilla scutellariae.

[0044] Experimental procedures

[0045] (1) 70 mice of normal weight were divided into 7 groups, including a first control group (treated with mesalazine), a second control group (treated with normal saline), a third control group (treated with the drug of Comparative Example 1), and four experimental groups (treated with the compositions of Examples 1 to 4, respectively), with 10 mice in each group;

[0046] (2) The UC model was established by freely drinking 4% dextran sodium sulfate (DSS) solution;

[0047] (3) Each group of mice received the same dose of drugs from the first to third control groups and the four experimental groups for intervention treatment, with medication administered daily for 15 days, after which medication was suspended;

[0048] (4) Hematoxylin-eosin (HE) staining to observe colonic lesions;

[0049] Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and Western blot were used to detect the expression of mucin MUC in colon tissue.

[0050] Mouse feces were analyzed by 16S r RNA high-throughput sequencing to examine the impact of intestinal microorganisms.

[0051] The specific results are shown in Table 1 below.

[0052] The degree of improvement of lesions in a single mouse = (the degree of lesions detected on the first day - the degree of lesions on the day of detection) / the degree of lesions detected on the first day; the degree of improvement of lesions = the sum of the degrees of improvement of lesions in single mice in the same group / 10.

[0053] Table 1 is a first control group (treated with mesalazine), a second control group (treated with normal saline), a third control group (treated with the drug in comparative example 1), and four experimental groups (treated with the compositions of Examples 1 to 4, respectively), and a detection chart of the improvement degree of colon disease observed by hematoxylin-eosin (HE) staining.

[0054]

[0055]

[0056] From the data in Table 1, we can see that

[0057] Although the therapeutic effect of mesalazine treatment is fast, reaching 98% on the 15th day, the condition will recur after the drug is stopped. Therefore, long-term medication is required for mesalazine treatment, and the side effects are large; the five drugs of Indigo, Red Peony, Phellodendron, Alum, and Plum correspond to the five internal organs, and the five internal organs are treated together, and the influence of each other is combined. Bletilla striata and Eclipta prostrata cooperate with the previous five drugs to have the effects of tonifying deficiency, benefiting the spleen, astringing and stopping bleeding, and can effectively treat intestinal ulcers; Poria cocos is flat in nature, and can be used with red peony to alleviate the coldness of Indigo, Red Peony, Phellodendron, and Alum. Poria cocos is used in combination with red peony, and the two drugs are used together to regulate water and blood, which is greatly beneficial to the recovery of ulcerative colitis; Bletilla striata and Poria cocos both have dispersible colloids, which can cover the ulcer during enema to form a drug film and improve the cure rate; and in the subsequent treatment process, it will not cause recurrence of the disease, and the speed of its treatment effect and the good degree of recovery after treatment are better than the effect of using the drug in Comparative Example 1 for treatment.

[0058] It should be noted that:

[0059] The above-mentioned embodiments only express the specific implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the protection scope of the present application. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the technical solution concept of the present application, and these all belong to the protection scope of the present application.

Claims

1. A Chinese medicine composition for treating ulcerative colitis, characterized in that: By weight, it is composed of the following raw materials: 8-12 parts of Indigo Naturalis; 25-32 parts of Red Peony Root; 18-23 parts of Phellodendron Amurense; 15-22 parts of Alum; 28-31 parts of Prunus Mume; 8-10 parts of Bletilla striata; 8-10 parts of Eclipta Prostrata; 12-15 parts of Poria.

2. A Chinese medicine composition for treating ulcerative colitis according to claim 1, characterized in that: The drug is composed of the following raw materials by weight: 12 parts of Indigo Naturalis, 32 parts of Red Peony Root, 23 parts of Phellodendron Amurense, 22 parts of Alum, 31 parts of Prunus Mume, 10 parts of Bletilla striata, 10 parts of Eclipta Prostrata and 15 parts of Poria.

3. A Chinese medicinal liquid for treating ulcerative colitis, characterized in that: The composition is prepared from the Chinese medicine composition for treating ulcerative colitis according to claim 1.

4. A method for preparing a Chinese medicinal liquid for treating ulcerative colitis, characterized in that: The following steps are involved: Step 1: Wash and crush the Chinese medicine composition for treating ulcerative colitis according to claim 1, and add water to immerse the medicine; Step 2: Soak for 30-40 minutes, heat to boiling, maintain boiling for 60-80 minutes, filter, and obtain the first filter residue and the first filtrate; Step 3: Add water to submerge the first filter residue, heat to boiling, maintain boiling for 40 to 60 minutes, filter, and obtain the second filter residue and the second filtrate; Step 4: Evenly mix the first filtrate and the second filtrate, and boil and concentrate them to 1 / 3 to 1 / 3.5 of the original volume to obtain a Chinese medicine solution for treating ulcerative colitis.

5. An application of a Chinese medicine composition for treating ulcerative colitis, characterized in that: The Chinese medicine composition for treating ulcerative colitis according to claim 1 is used for preparing a medicine for treating ulcerative colitis by oral administration or enema.

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