Anti-inflammatory efficacy of a honey-refined Chuanbei loquat syrup preparation

Abstract

The invention discloses a honey-refined fritillary and loquat syrup preparation with anti-inflammatory effects. The preparation comprises the following components in parts by mass: 10-20 parts of honey, 60-80 parts of fritillary bulbs, 200-300 parts of loquat leaves, 60-80 parts of platycodon grandiflorum, 0.4-1.5 parts of menthol, 40-60 parts of turmeric, 20-30 parts of green tea, and 30-40 parts of liquorice. The honey-refined fritillary and loquat syrup has significant anti-inflammatory effects, can effectively inhibit the production and release of inflammatory factors, alleviate inflammatory symptoms, has no toxic side effects, has a good taste, and is easy to eat.

Description

Technical Field

[0001] The invention relates to the technical field of health care and traditional Chinese medicine, and in particular to the anti-inflammatory effect of a honey-refined Chuanbei loquat syrup preparation. Background Art

[0002] Chuanbei Loquat Syrup, prepared according to the Chinese Pharmacopoeia (2020 Edition), is an oral syrup formulated with a traditional Chinese medicine process. It is used to treat coughs with yellow or unpleasant sputum, sore throats, chest pain, colds, coughs, and chronic bronchitis caused by wind-heat invading the lungs and internal fire. Years of clinical validation have proven its stable quality and proven efficacy, making it a commonly used medication for these conditions in both clinical and home settings. Other similar oral preparations include pills and tablets, which have similar therapeutic effects and efficacy, with no significant differences.

[0003] Inflammation is the body's immune system's response to external stimuli, but excessive inflammation can lead to tissue damage and disease. Currently, many anti-inflammatory drugs are available on the market, but most suffer from side effects and drug resistance. Chuanbei loquat syrup primarily relieves coughs, but its anti-inflammatory properties remain to be explored.

[0004] Therefore, it is necessary to provide a technical solution for the anti-inflammatory effect of a honey-refined Chuanbei loquat syrup. Summary of the Invention

[0005] In view of this, the present application provides an anti-inflammatory effect of honey-refined Chuanbei loquat syrup, which is used to solve the problem of proving how to improve the anti-inflammatory effect of honey-refined Chuanbei loquat syrup.

[0006] In order to achieve the above technical objectives, this application adopts the following technical solutions:

[0007] In a first aspect, the present application provides a honey-refined Fritillaria cirrhosa and loquat syrup, comprising the following components in parts by mass: 10-20 parts of honey, 60-80 parts of Fritillaria cirrhosa, 200-300 parts of loquat leaves, 60-80 parts of Platycodon grandiflorum, 0.4-1.5 parts of menthol, 40-60 parts of Curcuma aromatica, 20-30 parts of green tea, and 30-40 parts of licorice.

[0008] Preferably, the mass ratio of turmeric to green tea is 6:2-3.

[0009] Preferably, the mass ratio of turmeric to honey is 6:1-2.

[0010] Preferably, the mass ratio of licorice to loquat leaves is 4:20-25.

[0011] In a second aspect, a method is provided for using a honey-refined Chuanbei loquat syrup in anti-inflammatory foods and / or medicines.

[0012] Preferably, the honey-refined Fritillaria and Loquat syrup is used to reduce inflammatory leukocytes.

[0013] Preferably, the honey-refined Chuanbei loquat syrup is used to regulate the TNF-α content in serum.

[0014] Preferably, the honey-refined Fritillaria and Loquat syrup is used to regulate the 1L-1β content in serum.

[0015] The preparation method of the honey-refined Chuanbei loquat syrup of the present invention comprises the following steps:

[0016] S1. Grind Fritillaria cirrhosa into powder and soak in 30-50% ethanol solution to obtain a leaking solution;

[0017] S2. Decoction of other herbs in the prescription for 12-36 hours and then filtering to obtain a filtrate;

[0018] S3. The filtrate is concentrated under reduced pressure to obtain an extract of medicinal materials having a water content of 10-20%. Honey and other excipients are then added to the extract, heated again, and stirred to obtain a honey-refined Chuanbei loquat syrup.

[0019] The anti-inflammatory effects of the syrups described in the Examples and Comparative Examples were tested using animal experiments. The testing mechanism involved spraying 15% ammonia directly onto the rat pharynx, causing the rat pharyngeal tissue to appear dark red and dull, with increased mucous secretions, indicating a state of acute congestion and swelling, indicating successful establishment of an acute pharyngitis model. In acute pharyngitis, the body produces an acute inflammatory response, which leads to elevated levels of white blood cells and inflammatory mediators. Changes in white blood cell counts and inflammatory mediators TNF-α and IL-1β in the blood of the experimental and model groups after acute pharyngitis were compared to determine whether the composition had an inhibitory effect on acute pharyngitis.

[0020] The beneficial effects of the present application are as follows: the honey-refined Fritillaria and loquat syrup of the present invention has a significant anti-inflammatory effect, can effectively inhibit the production and release of inflammatory factors, alleviate inflammatory symptoms, has no toxic side effects, has a good taste, and is easy to use. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0022] The present application provides a honey-refined Fritillaria cirrhosa and loquat syrup, comprising the following components in parts by mass: 10-20 parts of honey, 60-80 parts of Fritillaria cirrhosa, 200-300 parts of loquat leaves, 60-80 parts of Platycodon grandiflorum, 0.4-1.5 parts of menthol, 40-60 parts of Curcuma aromatica, 20-30 parts of green tea, and 30-40 parts of licorice.

[0023] In this application, the functions of each component are as follows:

[0024] Honey: 1. Used to make refined honey. Honey is heated to remove impurities and moisture, destroy enzymes, kill microorganisms, and enhance adhesion, resulting in a honey-refined Chuanbei loquat syrup formulation. 2. Relieves inflammatory responses. First, honey is rich in antioxidants, such as phenolic compounds and enzymes, which can neutralize free radicals and reduce inflammatory responses caused by oxidative stress. Second, honey is rich in enzymes such as glucose oxidase and lactic acid kinase, which can enhance the activity of enzyme systems in the human body and promote anti-inflammatory responses. In addition, honey contains some special anti-inflammatory molecules that have the effect of directly inhibiting the activity of inflammatory cells. At the same time, honey can also enhance immunity and improve the body's ability to resist inflammation. 3. It synergizes with turmeric to enhance anti-inflammatory effects.

[0025] Fritillaria cirrhosa and loquat leaves: have the effects of clearing heat and moistening the lungs, resolving phlegm and relieving cough, and are used to treat symptoms such as lung heat and dry cough, dry cough with little phlegm, etc.

[0026] Licorice: 1. Inhibits the production and release of multiple inflammatory factors and reduces inflammatory responses; 2. Licorice works synergistically with loquat leaves, Fritillaria cirrhosa, etc. to enhance the overall anti-inflammatory effect.

[0027] Curcuma: 1. Promotes blood circulation and relieves pain, promotes qi and relieves depression, clears the heart and cools blood, promotes bile secretion and reduces jaundice; 2. Used in combination with honey, it synergistically enhances the anti-inflammatory effect; 3. Used in combination with green tea, it synergistically enhances the anti-inflammatory effect.

[0028] The aforementioned ingredients demonstrate two synergistic effects that enhance the anti-inflammatory effects of honey-refined Chuanbei and loquat syrup: turmeric and honey, and turmeric and green tea. This is because the anti-inflammatory substances in turmeric synergize with both honey and green tea to enhance their anti-inflammatory effects.

[0029] The synergistic mechanism of turmeric and honey is: turmeric contains a large amount of curcumin, which can inhibit the production of inflammatory mediators, reduce inflammatory responses, and regulate inflammation-related signal pathways. The fructose, glucose and minerals in honey can also play an antibacterial and anti-inflammatory role. When turmeric and honey are used together, their anti-inflammatory effects can enhance each other. On the one hand, the ingredients in honey can promote the absorption of curcumin and improve its anti-inflammatory effect. On the other hand, curcumin can also enhance the anti-inflammatory effect of honey and further reduce the damage of inflammation to the body.

[0030] The synergistic mechanism of turmeric and green tea is: turmeric contains a large amount of curcumin, while green tea contains a large amount of catechins. Both catechins and curcumin have antioxidant effects, which can scavenge free radicals and reduce the damage of oxidative stress to tissues. The antioxidant effect can further inhibit the inflammatory response and reduce the damage of inflammation to the body. Catechins and curcumin can inhibit the production of inflammatory factors, such as cytokines and chemokines, and promote the production of anti-inflammatory factors, such as interleukin-10 and transforming growth factor, so their combined use can synergistically enhance the anti-inflammatory effect.

[0031] In some embodiments, the mass ratio of turmeric to green tea is 6:2-3; the mass ratio of turmeric to honey is 6:1-2. Since turmeric, green tea, and honey all synergistically enhance anti-inflammatory effects, the required amount of turmeric is relatively large.

[0032] In some embodiments, the mass ratio of licorice to loquat leaves is 4:20-25. If too much licorice is used, the presence of honey will enhance the sweetness and the taste will be bad; if too little licorice is used, licorice and loquat leaves will not have a synergistic anti-inflammatory effect.

[0033] The present invention provides a method for preparing honey-refined Chuanbei loquat syrup, comprising the following steps:

[0034] S1. The Fritillaria cirrhosa, loquat leaves, Platycodon grandiflorum, menthol, turmeric, green tea, and licorice are crushed into powder, and the extractant is added and soaked to obtain a mixture;

[0035] S2. The mixture is boiled and filtered to obtain a filtrate;

[0036] S3. Heat the concentrated filtrate to obtain a concentrated solution, then add honey to the concentrated solution and stir to mix well to obtain the honey-refined Chuanbei loquat syrup.

[0037] In some embodiments, the extractant is a 30-50% ethanol aqueous solution, and ethanol can effectively extract effective ingredients such as curcumin and catechin.

[0038] In step S2, the decoction temperature is 80-90°C for 12-36 hours. In step S3, the heating temperature is 95-100°C. Because the decoction time in step S2 is long, the decoction temperature should not be too high to avoid the loss of active ingredients in the traditional Chinese medicine. Step S3, on the other hand, aims to concentrate the herb, requiring a higher temperature to evaporate water and ethanol.

[0039] In some embodiments, the water content of the concentrate is 10-20%. If the water content is too high, the syrup will be too thick and difficult to swallow. If the water content is too low, bacteria will easily grow, which is not conducive to storage and transportation.

[0040] In step S3, the purpose of heating again after adding honey is to remove impurities and moisture, destroy enzymes, kill microorganisms, and enhance adhesion.

[0041] The present application provides an application of honey-refined Chuanbei loquat syrup in anti-inflammatory food and / or medicine. The honey-refined Chuanbei loquat syrup can be used as an additive in food and medicine, or taken directly.

[0042] Preferably, the honey-refined Fritillaria and Loquat syrup is used to reduce inflammatory leukocytes.

[0043] Preferably, the honey-refined Chuanbei loquat syrup is used to regulate the TNF-α content in serum.

[0044] Preferably, the honey-refined Fritillaria and loquat syrup is used to regulate the IL-1β content in serum.

[0045] Preferably, the application of the honey-refined Chuanbei loquat syrup in regulating the IgE content in serum

[0046] Preferably, the application of the honey-refined Chuanbei loquat syrup in regulating the IL-4 content in serum

[0047] The present invention is further described below through specific examples.

[0048] Example 1

[0049] A honey-refined Fritillaria cirrhosa and loquat syrup comprises the following components in parts by weight: 20 parts of honey, 80 parts of Fritillaria cirrhosa, 200 parts of loquat leaves, 80 parts of Platycodon grandiflorum, 1.5 parts of menthol, 60 parts of Curcuma aromatica, 30 parts of green tea, and 40 parts of liquorice.

[0050] A method for preparing honey-refined Chuanbei loquat syrup comprises the following steps:

[0051] S1. Crush Fritillaria cirrhosa into powder, add 50% ethanol aqueous solution and soak for 24 hours to obtain a percolate;

[0052] S2. The mixture was boiled for 36 hours, and then filtered to obtain a filtrate;

[0053] S3. The filtrate is concentrated under reduced pressure until a concentrate having a water content of 10% is obtained, honey is added to the concentrate, and the mixture is heated at 100°C and stirred to obtain a honey-refined Chuanbei loquat syrup.

[0054] Example 2

[0055] A honey-refined Chuanbei loquat syrup, the other contents of which are the same as those of Example 1, except that the amount of Curcuma aromatica is 40 parts.

[0056] Example 3

[0057] A honey-refined Chuanbei loquat syrup, the other contents of which are the same as those of Example 1, except that the amount of liquorice used is 20 parts.

[0058] Example 4

[0059] A honey-refined Fritillaria cirrhosa and loquat syrup comprises the following components in parts by weight: 10 parts of honey, 60 parts of Fritillaria cirrhosa, 300 parts of loquat leaves, 60 parts of Platycodon grandiflorum, 1 part of menthol, 50 parts of Curcuma aromatica, 20 parts of green tea, and 30 parts of liquorice.

[0060] Comparative Example 1

[0061] A honey-refined Chuanbei loquat syrup is the same as Example 1 in other contents, except that Curcuma zedoaria is not included and the amount of honey used is 80 parts.

[0062] Comparative Example 2

[0063] A Sichuan fritillary loquat syrup, the other contents of which are the same as those of Example 1, except that honey is not included and the amount of turmeric is 80 parts.

[0064] Comparative Example 3

[0065] A honey-refined Chuanbei loquat syrup, the other contents of which are the same as those of Example 1, except that green tea is not included and the amount of turmeric is 90 parts.

[0066] Comparative Example 4

[0067] A honey-refined Chuanbei loquat syrup, the other contents of which are the same as those of Example 1, except that turmeric is not included and the amount of green tea used is 90 parts.

[0068] Comparative Example 5

[0069] A Sichuan fritillary loquat syrup, the other contents of which are the same as those of Example 1, except that neither turmeric nor honey is included.

[0070] Comparative Example 6

[0071] A Sichuan fritillary loquat syrup, the other contents of which are the same as those of Example 1, except that neither turmeric nor green tea is included.

[0072] Evaluation Test

[0073] The anti-inflammatory effects of the syrups described in the Examples and Comparative Examples were tested using animal experiments. The testing mechanism involved spraying 15% ammonia directly onto the rat pharynx, causing the rat pharyngeal tissue to appear dark red and dull, with increased mucous secretions, indicating a state of acute congestion and swelling, indicating successful establishment of an acute pharyngitis model. In acute pharyngitis, the body produces an acute inflammatory response, which leads to elevated levels of white blood cells and inflammatory mediators. Changes in white blood cell counts and inflammatory mediators TNF-α and IL-1β in the blood of the experimental and model groups after acute pharyngitis were compared to determine whether the composition had an inhibitory effect on acute pharyngitis.

[0074] Animal grouping: Clean-grade SD rats, weighing 180-220 g, half male and half female, were randomly divided into 6 groups, namely, blank group, model group (ammonia modeling), Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group, and Comparative Example 6 group, with 10 animals in each group.

[0075] The specific administration method is as follows: blank group, rats were given an equal volume of distilled water by gavage once a day for 5 consecutive days; model group, rats were given an equal volume of distilled water by gavage once a day for 5 consecutive days; Example and comparative example groups: rats were given 1.2 g / kg of the composition by gavage at 10 mL / kg once a day for 5 consecutive days.

[0076] Test: 0.5 hours after administration on the fifth day, whole blood was collected from the rats. A portion was used for routine white blood cell testing, and a portion was used to extract serum. The levels of TNF-α, IL-1β, IgE, and IL-4 in the rat serum were detected using commercially available ELISA kits.

[0077] The mean values ​​of each group were calculated, and the results of white blood cell count, serum TNF-α, IL-1β, IgE, and IL-4 are shown in Table 1.

[0078] Table 1 Anti-inflammatory test evaluation results

[0079]

[0080] As shown in Table 1, the syrups in the embodiments and comparative examples all have certain anti-inflammatory effects, wherein the anti-inflammatory effect of the honey-refined Chuanbei loquat syrup of the present application is better than the anti-inflammatory effect of the Chuanbei loquat syrup in the comparative example. Taking Comparative Example 5 as a reference value, Example 1 uses honey and turmeric at the same time, while Comparative Example 1 and Comparative Example 2 only use one of the components, but the sum of the decrease in white blood cells in Comparative Example 1 and Comparative Example 2 is less than the decrease in Example 1, indicating that honey and turmeric have a synergistic effect of reducing the number of white blood cells and achieving a synergistic effect of enhancing the anti-inflammatory effect. Similarly, taking Comparative Example 6 as a reference value, it can be seen from the data of Comparative Example 1, Comparative Example 3, and 4 that turmeric and green tea also have a synergistic effect of enhancing the anti-inflammatory effect.

[0081] The above are only preferred specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or replacements that can be easily thought of by any technician familiar with this technical field within the technical scope disclosed by the present invention should be covered by the scope of protection of the present invention.

Claims

1. A honey-refined Chuanbei loquat syrup, characterized in that: The preparation comprises the following components by mass: 20 parts of honey, 80 parts of Fritillaria cirrhosa, 200 parts of loquat leaves, 80 parts of Platycodon grandiflorum, 1.5 parts of menthol, 60 parts of Curcuma aromatica, 30 parts of green tea and 40 parts of liquorice.

2. Use of the honey-refined Chuanbei loquat syrup according to claim 1 in preparing a medicine for treating acute pharyngitis.

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