Preparation method and application of a cyanobacterial cell wall broken organic material loaded on biomass charcoal activated by Fe2(SO4)3 and KOH together

Abstract

The present application relates to a kind of Fe2 (SO4) 3 with KOH together activated biomass charcoal load cyanobacteria broken shell organic material preparation method and application, the biomass charcoal material of the present application is prepared by Fe2 (SO4) 3 and KOH together activation, both are pore former, increase the porosity of biomass charcoal surface, together improve the adsorption capacity of biomass charcoal.Fe2 (SO4) 3 provides iron element for biomass charcoal, Fe (OH) 3 is reacted with carbon material to generate iron particle at high temperature, and amorphous carbon is catalytically converted into graphitized carbon.Algae liquid is broken, and after reducing algal toxin, its phosphorus element is complexed with biomass charcoal by iron ion, and at the same time, the C=O of biomass charcoal and the-NH2 in algal liquid form nitrogen organic layer, to prepare new slow-release fertilizer.Under the action of ultrasound, modified biomass charcoal is used as sonosensitizer, and after ultrasonic treatment, the C=O of biomass charcoal has obvious increase, and the adsorption capacity of ammonia nitrogen is strengthened.The material not only simple process, low cost, but also absorption and release effect to N, P is good, has higher research value and application advantage.

Description

Technical Field

[0001] This invention relates to a method for preparing and applying a biochar-supported cyanobacteria-based organic material with cell wall disruption function, which is co-activated by Fe2(SO4)3 and KOH, and belongs to the field of water treatment technology. Background Technology

[0002] Modern agriculture relies heavily on the widespread use of chemical fertilizers to improve soil fertility and crop yields. However, the use of chemical fertilizers inevitably leads to nutrient leaching into nearby water bodies, particularly nitrogen and phosphorus, causing nutrient pollution and threatening the health of aquatic organisms and humans. A significant reason for these problems is the low utilization efficiency of chemical fertilizers. In recent years, researchers both domestically and internationally have increasingly focused on developing slow-release fertilizers to improve nutrient utilization efficiency. Compared to conventional chemical fertilizers, slow-release fertilizers deliver nutrients more slowly and for a longer duration, aiming to better meet the needs of crops. To date, biochar is widely considered one of the most promising fertilizer carriers for delaying nutrient release due to its dense porous structure and abundant functional groups. Furthermore, biochar is widely regarded as a promising soil conditioner because numerous studies have found that adding biochar can improve soil physical structure, increase soil cation exchange capacity, and enhance soil organic carbon content. Therefore, biochar-based slow-release fertilizers have great potential to improve fertilizer utilization efficiency and remediate soil conditions. Summary of the Invention

[0003] The purpose of this invention is to address the aforementioned problems by providing a method for preparing and applying a biochar-supported cyanobacterial cell-wall-breaking organic material co-activated by Fe2(SO4)3 and KOH.

[0004] To achieve the above-mentioned objectives, the technical solution adopted by this invention is as follows:

[0005] A method for preparing a biochar-supported cyanobacterial cell-wall-broken organic material co-activated by Fe2(SO4)3 and KOH, characterized by comprising the following steps:

[0006] (1) Wash the bamboo charcoal powder with deionized water 3 to 5 times and then dry it in an oven at 70°C.

[0007] (2) Prepare Fe2(SO4)3 solution with deionized water;

[0008] (3) Take the bamboo charcoal powder washed in step (1) and put it into the Fe2(SO4)3 solution prepared in step (2). Stir and heat it in a water bath at 80°C for 2 hours, then take it out and put it into an oven at 70°C to dry.

[0009] (4) The material obtained in step (3) is mixed and ground with KOH and then placed in a tube furnace for calcination for 2 hours.

[0010] (5) Grind the material obtained in step (4) through a 0.15mm sieve;

[0011] (6) Centrifuge the cyanobacteria solution (centrifugation speed is 3600 rpm / min), then discard the supernatant, collect the algal cells, add deionized water, and centrifuge and wash once more. Let the washed algal solution stand for 15-20 minutes to allow the algal cells to stabilize in the deionized water.

[0012] (7) Use an optical microscope to count the algal cells in the cleaned algal solution in step (6) to determine the algal cell concentration;

[0013] (8) Take 20 ml of the algal solution from step (7) and mix it with the material obtained in step (5), and sonicate it in a 40 kHz ultrasonic machine for 10 min.

[0014] (9) The material obtained in step (8) is separated and dried in an oven at 70°C to obtain Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacteria cell wall broken organic material.

[0015] In step (2), the concentration of Fe2(SO4)3 solution is 1-6 mol / L.

[0016] In step (4), the mass ratio of the material to KOH is 1:1 to 6.

[0017] In step (7), the concentration of the cyanobacteria solution is 1×10⁻⁶. 6 ~1×10 7 per ml.

[0018] In step (8), the material is 2 to 10 mg.

[0019] Application of a biochar-supported cyanobacterial cell wall-breaking organic material co-activated by Fe2(SO4)3 and KOH in crop cultivation.

[0020] This invention employs an advanced and scientific method. The objectives of this invention are twofold: firstly, to provide a method for preparing a biochar-supported cyanobacteria-based organic material with cell-wall-breaking properties activated by Fe2(SO4)3 and KOH; and secondly, to apply this Fe2(SO4)3 and KOH-activated biochar-supported cyanobacteria-based organic material with cell-wall-breaking properties. The biochar material of this invention is prepared by co-activation with Fe2(SO4)3 and KOH, both of which act as pore-forming agents, increasing the porosity of the biochar surface and jointly enhancing its adsorption capacity. Simultaneously, Fe2(SO4)3 provides iron to the biochar, and Fe(OH)3 reacts with the carbon material at high temperatures to generate iron microparticles, catalytically converting amorphous carbon into graphitized carbon. After cell-wall breaking of the algal solution and reduction of algal toxins, the phosphorus in the algal solution is used to complex with the biochar through iron ions. Simultaneously, the C=O of the biochar forms a nitrogen-containing organic layer with the -NH2 in the algal solution, thus preparing a novel slow-release fertilizer. Under the action of ultrasound, the modified biochar, as a sound-sensitive agent, showed a significant increase in C=O after ultrasound, which enhanced its adsorption capacity for ammonia nitrogen.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0022] 1. The raw material of this invention, bamboo charcoal, is widely available from blue-green algae, and the preparation method is simple, economical, and quick.

[0023] 2. The biochar material co-activated by Fe2(SO4)3 and KOH provided by the present invention not only has excellent porosity, but also acts as a sound-sensitive agent to enhance the adsorption capacity of nitrogen, phosphorus and potassium.

[0024] 3. The Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacteria cell wall-breaking organic material prepared by the present invention can not only effectively utilize cyanobacteria, but also effectively utilize the excellent adsorption capacity of biochar.

[0025] 4. The material preparation method of this invention is simple, has good processing effect, wide range of applications, and low cost, and has high research value and unique application advantages.

[0026] This invention utilizes biochar activation to enhance its adsorption capacity. By co-ultrasonication with cyanobacteria, the algal solution is disrupted, reducing algal toxins. The phosphorus in the biochar complexes with iron ions, while the C=O atoms of the biochar form a nitrogen-containing organic layer with the -NH2 atoms in the algal solution, thus preparing a novel slow-release fertilizer. This material is not only simple to process and low in cost, but also exhibits excellent absorption and release of N and P, demonstrating high research value and application advantages. Attached Figure Description

[0027] Figure 1 The results of granulation of biochar-supported cyanobacterial cell-wall-broken organic materials co-activated by Fe2(SO4)3 and KOH;

[0028] Figure 2 Image showing the precipitation effect after co-ultrasonic mixing of biochar and cyanobacteria;

[0029] Figure 3 The graph shows the biochar removal rate of cyanobacteria after being activated with 4 mol / L Fe2(SO4)3 and KOH in Examples 1-6.

[0030] Figure 4 Here is a SEM image of the material in Example 1;

[0031] Figure 5 This is the BET diagram of the materials in Example 6. Detailed Implementation

[0032] The present invention will be further described in detail below with reference to examples and accompanying drawings. Unless otherwise specified, the experimental methods described in the following embodiments are conventional methods; the reagents described, unless otherwise specified, are commercially available.

[0033] Example 1

[0034] The specific steps for preparing Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacterial cell-wall-broken organic material in this embodiment are as follows:

[0035] (1) Wash the bamboo charcoal powder with deionized water 3 to 5 times and then dry it in an oven at 70°C.

[0036] (2) Prepare a 1 mol / L Fe2(SO4)3 solution using deionized water.

[0037] (3) Take the bamboo charcoal powder washed in step (1) and put it into the solution prepared in step (2). Stir and heat it in a water bath at 80°C for 2 hours, then take it out and put it into an oven at 70°C to dry.

[0038] (4) The material obtained in step (3) is mixed and ground with KOH at a mass ratio of 1:1, and then placed in a tube furnace and fired at 400°C for 2 hours.

[0039] (5) Grind the material obtained in step (4) through a 0.15mm sieve.

[0040] (6) Centrifuge the cyanobacteria solution (3600 rpm / min), then discard the supernatant, collect the algal cells, add deionized water, and centrifuge and wash once more. Let the washed algal solution stand for 15-20 minutes to allow the algal cells to stabilize in the deionized water.

[0041] (7) Count the algal cells in the cleaned algal solution from step (6) using an optical microscope to determine the algal cell concentration as 2 × 10⁻⁶. 6 per L.

[0042] (8) Take 20 ml of the algal solution from step (7) and mix it with the material obtained in step (5), and sonicate it in a 40 kHz ultrasonic machine for 10 min.

[0043] (9) The material obtained in step (8) is separated and dried in an oven at 70°C to obtain Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacteria cell wall broken organic material.

[0044] The algal strain selected was *Microcystis aeruginosa*, purchased from a freshwater algae database, catalog number FACHB-912. It was cultured in BG11 medium at 25°C. Biomass was determined by measuring the density of *Microcystis aeruginosa* cells using hemocytometrics. The removal rate formula used in the experiment was:

[0045] Removal rate = (C0-C) / C0×100%, where C0 and C are the number of algal cells before and after the reaction, respectively.

[0046] The experiment was conducted in a 40kHz ultrasonic bath. 20ml of diluted algal solution was taken and acoustic sensitizers (200, 300, 400mg / L) were added. The ultrasonic time was set to 10min. Samples were taken and measured separately. The control group was without acoustic sensitizers.

[0047] Example 2

[0048] The specific steps for preparing Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacterial cell-wall-broken organic material in this embodiment are as follows:

[0049] (1) Wash the bamboo charcoal powder with deionized water 3 to 5 times and then dry it in an oven at 70°C.

[0050] (2) Prepare a 2 mol / L Fe2(SO4)3 solution using deionized water.

[0051] (3) Take the bamboo charcoal powder washed in step (1) and put it into the solution prepared in step (2). Stir and heat it in a water bath at 80°C for 2 hours, then take it out and put it into an oven at 70°C to dry.

[0052] (4) The material obtained in step (3) is mixed and ground with KOH at a mass ratio of 1:1, and then placed in a tube furnace and fired at 400°C for 2 hours.

[0053] (5) Grind the material obtained in step (4) through a 0.15mm sieve.

[0054] (6) Centrifuge the cyanobacteria solution (3600 rpm / min), then discard the supernatant, collect the algal cells, add deionized water, and centrifuge and wash once more. Let the washed algal solution stand for 15-20 minutes to allow the algal cells to stabilize in the deionized water.

[0055] (7) Count the algal cells in the cleaned algal solution from step (6) using an optical microscope to determine the algal cell concentration as 2 × 10⁻⁶. 6 per L.

[0056] (8) Take 20 ml of the algal solution from step (7) and mix it with the material obtained in step (5), and sonicate it in a 40 kHz ultrasonic machine for 10 min.

[0057] (9) The material obtained in step (8) is separated and dried in an oven at 70°C to obtain Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacteria cell wall broken organic material.

[0058] The algal strain selected was *Microcystis aeruginosa*, purchased from a freshwater algae database, catalog number FACHB-912. It was cultured in BG11 medium at 25°C. Biomass was determined by measuring the density of *Microcystis aeruginosa* cells using hemocytometrics. The removal rate formula used in the experiment was:

[0059] Removal rate = (C0-C) / C0×100%, where C0 and C are the number of algal cells before and after the reaction, respectively.

[0060] The experiment was conducted in a 40kHz ultrasonic bath. 20ml of diluted algal solution was taken and acoustic sensitizers (200, 300, 400mg / L) were added. The ultrasonic time was set to 10min. Samples were taken and measured separately. The control group was without acoustic sensitizers.

[0061] Example 3

[0062] The specific steps for preparing Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacterial cell-wall-broken organic material in this embodiment are as follows:

[0063] (1) Wash the bamboo charcoal powder with deionized water 3 to 5 times and then dry it in an oven at 70°C.

[0064] (2) Prepare a 4 mol / L Fe2(SO4)3 solution using deionized water.

[0065] (3) Take the bamboo charcoal powder washed in step (1) and put it into the solution prepared in step (2). Stir and heat it in a water bath at 80°C for 2 hours, then take it out and put it into an oven at 70°C to dry.

[0066] (4) Mix and grind the material obtained in step (3) with KOH at a mass ratio of 1:1, and then place it in a tube furnace and calcine at 400°C for 2 hours.

[0067] (5) Grind the material obtained in step (4) through a 0.15mm sieve.

[0068] (6) Centrifuge the cyanobacteria solution (3600 rpm / min), then discard the supernatant, collect the algal cells, add deionized water, and centrifuge and wash once more. Let the washed algal solution stand for 15-20 minutes to allow the algal cells to stabilize in the deionized water.

[0069] (7) Count the algal cells in the cleaned algal solution from step (6) using an optical microscope to determine the algal cell concentration as 2 × 10⁻⁶. 6 per L.

[0070] (8) Take 20 ml of the algal solution from step (7) and mix it with the material obtained in step (5), and sonicate it in a 40 kHz ultrasonic machine for 10 min.

[0071] (9) The material obtained in step (8) is separated and dried in an oven at 70°C to obtain Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacteria cell wall broken organic material.

[0072] The algal strain selected was *Microcystis aeruginosa*, purchased from a freshwater algae database, catalog number FACHB-912. It was cultured in BG11 medium at 25°C. Biomass was determined by measuring the density of *Microcystis aeruginosa* cells using hemocytometrics. The removal rate formula used in the experiment was:

[0073] Removal rate = (C0-C) / C0×100%, where C0 and C are the number of algal cells before and after the reaction, respectively.

[0074] The experiment was conducted in a 40kHz ultrasonic bath. 20ml of diluted algal solution was taken and acoustic sensitizers (200, 300, 400mg / L) were added. The ultrasonic time was set to 10min. Samples were taken and measured separately. The control group was without acoustic sensitizers.

[0075] Example 4

[0076] The specific steps for preparing Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacterial cell-wall-broken organic material in this embodiment are as follows:

[0077] (1) Wash the bamboo charcoal powder with deionized water 3 to 5 times and then dry it in an oven at 70°C.

[0078] (2) Prepare a 1 mol / L Fe2(SO4)3 solution using deionized water.

[0079] (3) Take the bamboo charcoal powder washed in step (1) and put it into the solution prepared in step (2). Stir and heat it in a water bath at 80°C for 2 hours, then take it out and put it into an oven at 70°C to dry.

[0080] (4) The material obtained in step (3) is mixed and ground with KOH at a mass ratio of 1:1, and then placed in a tube furnace and fired at 900°C for 2 hours.

[0081] (5) Grind the material obtained in step (4) through a 0.15mm sieve.

[0082] (6) Centrifuge the cyanobacteria solution (3600 rpm / min), then discard the supernatant, collect the algal cells, add deionized water, and centrifuge and wash once more. Let the washed algal solution stand for 15-20 minutes to allow the algal cells to stabilize in the deionized water.

[0083] (7) Count the algal cells in the cleaned algal solution from step (6) using an optical microscope to determine the algal cell concentration as 2 × 10⁻⁶. 6 per L.

[0084] (8) Take 20 ml of the algal solution from step (7) and mix it with the material obtained in step (5), and sonicate it in a 40 kHz ultrasonic machine for 10 min.

[0085] (9) The material obtained in step (8) is separated and dried in an oven at 70°C to obtain Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacteria cell wall broken organic material.

[0086] The algal strain selected was *Microcystis aeruginosa*, purchased from a freshwater algae database, catalog number FACHB-912. It was cultured in BG11 medium at 25°C. Biomass was determined by measuring the density of *Microcystis aeruginosa* cells using hemocytometrics. The removal rate formula used in the experiment was:

[0087] Removal rate = (C0-C) / C0×100%, where C0 and C are the number of algal cells before and after the reaction, respectively.

[0088] The experiment was conducted in a 40kHz ultrasonic bath. 20ml of diluted algal solution was taken and acoustic sensitizers (200, 300, 400mg / L) were added. The ultrasonic time was set to 10min. Samples were taken and measured separately. The control group was without acoustic sensitizers.

[0089] Example 5

[0090] The specific steps for preparing Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacterial cell-wall-broken organic material in this embodiment are as follows:

[0091] (1) Wash the bamboo charcoal powder with deionized water 3 to 5 times and then dry it in an oven at 70°C.

[0092] (2) Prepare a 2 mol / L Fe2(SO4)3 solution using deionized water.

[0093] (3) Take the bamboo charcoal powder washed in step (1) and put it into the solution prepared in step (2). Stir and heat it in a water bath at 80°C for 2 hours, then take it out and put it into an oven at 70°C to dry.

[0094] (4) The material obtained in step (3) is mixed and ground with KOH at a mass ratio of 1:1, and then placed in a tube furnace and fired at 900°C for 2 hours.

[0095] (5) Grind the material obtained in step (4) through a 0.15mm sieve.

[0096] (6) Centrifuge the cyanobacteria solution (3600 rpm / min), then discard the supernatant, collect the algal cells, add deionized water, and centrifuge and wash once more. Let the washed algal solution stand for 15-20 minutes to allow the algal cells to stabilize in the deionized water.

[0097] (7) Count the algal cells in the cleaned algal solution from step (6) using an optical microscope to determine the algal cell concentration as 2 × 10⁻⁶. 6 per L.

[0098] (8) Take 20 ml of the algal solution from step (7) and mix it with the material obtained in step (5), and sonicate it in a 40 kHz ultrasonic machine for 10 min.

[0099] (9) The material obtained in step (8) is separated and dried in an oven at 70°C to obtain Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacteria cell wall broken organic material.

[0100] The algal strain selected was *Microcystis aeruginosa*, purchased from a freshwater algae database, catalog number FACHB-912. It was cultured in BG11 medium at 25°C. Biomass was determined by measuring the density of *Microcystis aeruginosa* cells using hemocytometrics. The removal rate formula used in the experiment was:

[0101] Removal rate = (C0-C) / C0×100%, where C0 and C are the number of algal cells before and after the reaction, respectively.

[0102] The experiment was conducted in a 40kHz ultrasonic bath. 20ml of diluted algal solution was taken and acoustic sensitizers (200, 300, 400mg / L) were added. The ultrasonic time was set to 10min. Samples were taken and measured separately. The control group was without acoustic sensitizers.

[0103] Sound-sensitive agent concentration 0 200 300 400 Removal rate 56.76％ 56.29％ 66.96％ 87.91％

[0104] Example 6

[0105] The specific steps for preparing Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacterial cell-wall-broken organic material in this embodiment are as follows:

[0106] (1) Wash the bamboo charcoal powder with deionized water 3 to 5 times and then dry it in an oven at 70°C.

[0107] (2) Prepare a 4 mol / L Fe2(SO4)3 solution using deionized water.

[0108] (3) Take the bamboo charcoal powder washed in step (1) and put it into the solution prepared in step (2). Stir and heat it in a water bath at 80°C for 2 hours, then take it out and put it into an oven at 70°C to dry.

[0109] (4) The material obtained in step (3) is mixed and ground with KOH at a mass ratio of 1:1, and then placed in a tube furnace and fired at 900°C for 2 hours.

[0110] (5) Grind the material obtained in step (4) through a 0.15mm sieve.

[0111] (6) Centrifuge the cyanobacteria solution (3600 rpm / min), then discard the supernatant, collect the algal cells, add deionized water, and centrifuge and wash once more. Let the washed algal solution stand for 15-20 minutes to allow the algal cells to stabilize in the deionized water.

[0112] (7) Count the algal cells in the cleaned algal solution from step (6) using an optical microscope to determine the algal cell concentration as 2 × 10⁻⁶. 6 per L.

[0113] (8) Take 20 ml of the algal solution from step (7) and mix it with the material obtained in step (5), and sonicate it in a 40 kHz ultrasonic machine for 10 min.

[0114] (9) The material obtained in step (8) is separated and dried in an oven at 70°C to obtain Fe2(SO4)3 and KOH co-activated biochar-supported cyanobacteria cell wall broken organic material.

[0115] The algal strain selected was *Microcystis aeruginosa*, purchased from a freshwater algae database, catalog number FACHB-912. It was cultured in BG11 medium at 25°C. Biomass was determined by measuring the density of *Microcystis aeruginosa* cells using hemocytometrics. The removal rate formula used in the experiment was:

[0116] Removal rate = (C0-C) / C0×100%, where C0 and C are the number of algal cells before and after the reaction, respectively.

[0117] The experiment was conducted in a 40kHz ultrasonic bath. 20ml of diluted algal solution was taken and acoustic sensitizers (200, 300, 400mg / L) were added. The ultrasonic time was set to 10min. Samples were taken and measured separately. The control group was without acoustic sensitizers.

[0118] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A preparation method of a cyanobacterial cell wall-breaking organic material loaded on biomass charcoal co-activated by Fe2(SO4)3 and KOH, characterized in that, It comprises the following steps: (1) wash the bamboo charcoal powder with deionized water for 3-5 times, and then put it into an oven at 70°C for drying; (2) prepare a Fe2(SO4)3 solution with deionized water; (3) take the washed bamboo charcoal powder in step (1) and put it into the Fe2(SO4)3 solution prepared in step (2), and then stir and heat it in a water bath at 80°C for 2h, and then take it out and put it into an oven at 70°C for drying; (4) mix and grind the material obtained in step (3) with KOH, and then put it into a tube furnace for calcination for 2h; (5) grind the material obtained in step (4) through a 0.15mm sieve; (6) centrifuge the blue-green algae solution at a speed of 3600rpm, then discard the supernatant and collect the algae cells, and then add deionized water and centrifuge again for cleaning, and then let the cleaned algae solution stand for 15-20min to stabilize the algae cells in the deionized water; (7) count the algae cells in the cleaned algae solution in step (6) with an optical microscope to determine the concentration of the algae cells; (8) take 20ml of the algae solution in step (7) and mix it with the material obtained in step (5), and then ultrasonicate it in an ultrasonic machine at 40kHz for 10min; (9) separate the material obtained in step (8) and put it into an oven at 70°C for drying to obtain a Fe2(SO4)3 and KOH co-activated biomass charcoal loaded blue-green algae broken cell organic material.

2. The preparation method of the biomass charcoal loaded cyanobacterial cell wall- broken organic material co-activated by Fe2(SO4)3 and KOH according to claim 1, characterized in that, In step (2), the concentration of the Fe2(SO4)3 solution is 1-6mol / L.

3. The preparation method of the biomass charcoal loaded cyanobacterial cell wall- broken organic material co-activated by Fe2(SO4)3 and KOH according to claim 1, characterized in that, In step (4), the mass ratio of the material to KOH is 1:1-6.

4. The preparation method of the biomass charcoal loaded cyanobacterial cell wall- broken organic material co-activated by Fe2(SO4)3 and KOH according to claim 1, characterized in that, In step (7), the concentration of the blue-green algae liquid was 1 x 10 6 ~1 x 10 7 ~1 x 10 5. The preparation method of the biomass charcoal loaded cyanobacterial cell wall- broken organic material co-activated by Fe2(SO4)3 and KOH according to claim 1, characterized in that, In step (8), the material is 2-10mg.

6. A Fe2(SO4)3 and KOH co-activated biomass charcoal loaded blue-green algae broken cell organic material prepared by the method of any one of claims 1-5 for use in crop planting.

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