Method for extracting quercetin from artemisia scoparia, product and application thereof

Abstract

The present application relates to a kind of method for extracting quercetin in green sage and its product and application, the method comprises: green sage is mixed with the solution containing deep eutectic solvent, ultrasonic, and the extraction solution is obtained;The extraction solution is centrifuged, and the supernatant is filtered, concentrated, and obtained.The deep eutectic solvent can accelerate the destruction of the cell wall of green sage plant cell, increase the permeability of cell membrane, promote the high-efficiency outflow of quercetin in green sage, and the deep eutectic solvent itself has certain anti-inflammatory, bacteriostatic effect, without further separation, realize the efficient extraction of active ingredient, while exerting excellent antioxidant, anti-glycation, anti-inflammatory and bacteriostatic effect.

Description

Technical Field

[0001] This invention belongs to the field of material extraction technology, and relates to a method for extracting quercetin from Meconopsis, its products, and applications. Background Technology

[0002] *Meconopsis* is a genus of perennial herbs belonging to the Papaveraceae family. It includes several species such as *Meconopsis patula*, *Meconopsis yunnanensis*, *Meconopsis racemosa*, and *Meconopsis integrifolia*. *Meconopsis* plants have persistent leaf bases densely covered with pale brown stiff hairs. The leaves are basal, forming a rosette, narrowly obovate to narrowly oblanceolate, with an acute or obtuse apex and a gradually narrowed base forming a petiole, which is entire including the petiole. The scape is unbranched and covered with pale brown stiff hairs; the flowers are solitary at the apex of the scape, with sepals deciduous early; the corolla is predominantly yellow, pale blue, or purple, nearly bell-shaped, and petal-like. The capsule is elliptical or oblong-elliptical, densely covered with appressed stiff hairs. Currently, it typically grows in shady slope thickets or alpine meadows at altitudes of 2300-4600 m.

[0003] Meconopsis is a well-known alpine flower with medicinal properties. The whole plant can be used medicinally; it is cool in nature and has a sweet and astringent taste. It possesses properties of clearing heat and detoxifying, promoting diuresis, reducing inflammation, and relieving pain. It is listed in my country's Ministry of Health's Drug Standards. Currently, more than thirty traditional Chinese medicine preparations use Meconopsis. In various traditional Chinese medicine formulas, it can be used to treat cough, hepatitis, cholecystitis, pneumonia, tuberculosis, gastric ulcers, headaches, and edema.

[0004] The components reported in *Meconopsis* are mainly alkaloids, flavonoids, and volatile oils. Flavonoids are the main bioactive components that exert the medicinal and health-care effects of many medicinal plants. They exhibit strong absorption capacity for both ultraviolet and visible light, thus possessing antioxidant properties. The flavonoids isolated and identified from *Meconopsis* mainly include quercetin, dihydroquercetin, and cucurbitacin. Flavonoids possess excellent antibacterial, anti-inflammatory, soothing, repairing, and antioxidant effects, enabling *Meconopsis* extract to be used in the cosmetics industry.

[0005] Meconopsis extract has anti-inflammatory effects. Results show that total flavonoids from Meconopsis can scavenge hydroxyl free radicals and superoxide anions, inhibit the production of MDA in liver tissue, and resist oxidative hemolysis of erythrocytes, thus exhibiting free radical scavenging and anti-lipid peroxidation effects.

[0006] Quercetin possesses various biological activities, primarily antioxidant, antibacterial, anti-inflammatory, and anti-allergic effects. In vivo, quercetin exhibits strong free radical scavenging activity. It inhibits the activity of lipid oxidases in cell membranes, thereby suppressing lipid peroxidation and protecting cells from oxidative damage. Its molecule contains numerous phenolic hydroxyl groups, exhibiting the general properties of phenolic antioxidants. Its anti-inflammatory and anti-allergic mechanism involves inhibiting cellular lipoxygenase and cyclooxygenase, thus suppressing the synthesis of pro-inflammatory factors and achieving its anti-inflammatory and anti-allergic effects.

[0007] Currently, there are many extraction solvents used for quercetin, such as water extraction, acid extraction, alkali extraction, and organic solvent extraction. However, extraction using these traditional solvents has many problems, such as poor selectivity, many impurities, and low content of active ingredients. The resulting natural extracts have poor stability, insufficient activity, or weak targeting. Therefore, there is an urgent need to develop new, green, and efficient extraction solvents. Summary of the Invention

[0008] In view of the shortcomings of the prior art, the purpose of this invention is to provide a method for extracting quercetin from Meconopsis, as well as its products and applications.

[0009] To achieve this objective, the present invention employs the following technical solution:

[0010] In a first aspect, the present invention provides a method for extracting quercetin from Meconopsis, the method comprising:

[0011] (1) Mix Meconopsis with a solution containing a deep eutectic solvent and sonicate to obtain an extract;

[0012] (2) Centrifuge the extract, filter the supernatant, and concentrate it to obtain the final product.

[0013] The deep eutectic solvent described in this invention can accelerate the destruction of the cell walls of Meconopsis plant cells, increase the permeability of the cell membrane, and promote the efficient outflow of quercetin from Meconopsis. Moreover, the deep eutectic solvent itself has certain anti-inflammatory and antibacterial effects, eliminating the need for further separation. This achieves efficient extraction of active ingredients while exerting excellent antioxidant, anti-glycation, anti-inflammatory, and antibacterial effects.

[0014] Preferably, the deep eutectic solvent is obtained by reacting a hydrogen bond donor and a hydrogen bond acceptor.

[0015] Preferably, the hydrogen bond donor comprises propylene glycol.

[0016] Preferably, the hydrogen bond acceptor includes matrine.

[0017] This invention selects matrine as the hydrogen bond acceptor for the deep eutectic solvent (NADES). Matrine is prepared by isolating and processing the total alkaloids extracted from the mature seeds of *Sophora alopecuroides*, a plant belonging to the genus *Sophora* in the legume family. Traditional Chinese medicine considers matrine to be cold in nature and bitter in taste, possessing the functions of clearing heat and dampness, relieving jaundice and detoxifying, and promoting diuresis. In the field of skincare, it has antibacterial and anti-inflammatory effects, and can be used to treat eczema and dermatitis, effectively conditioning the skin and hair, making them moisturized and shiny.

[0018] This invention selects propylene glycol as the hydrogen bond donor for eutectic solvents (NADES). Propylene glycol is a slightly sweet, colorless, odorless, viscous, and hygroscopic straight-chain fatty alcohol liquid. Industrially, propylene glycol is commonly used as a wetting agent and solvent, and is also a major component of advanced antifreeze. In the food processing industry, propylene glycol fatty acid esters, formed by the reaction of propylene glycol with fatty acids, are mainly used as food emulsifiers. In pharmaceutical engineering, it can be used as a solvent to increase drug stability and is commonly used in the manufacture of various ointments and creams as a solvent and emulsifier. In the cosmetics industry, it is commonly used in face masks, toners, facial cleansers, serums, makeup removers, and moisturizing lotions.

[0019] This invention creatively discovers that the deep eutectic solvent obtained by reacting matrine and propylene glycol has a better extraction effect on quercetin and a better antioxidant effect.

[0020] Preferably, the mass percentage of the eutectic solvent in the solution containing the eutectic solvent is 10-90%, such as 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 7%, 80%, 85%, 90%, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0021] Preferably, the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:(0.2-5), where the specific values ​​in (0.2-5) can be selected from 0.2, 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, 4.8, 5, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0022] Preferably, the reaction temperature is 30-60℃ and the time is 1-8h.

[0023] The temperature can be selected from 30℃, 32℃, 35℃, 38℃, 40℃, 42℃, 45℃, 48℃, 50℃, 52℃, 55℃, 58℃, 60℃, etc., and the time can be selected from 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, 4.5h, 5h, 5.5h, 6h, 6.5h, 7h, 7.5h, 8h, etc. Other specific point values ​​within the above range can be selected, which will not be elaborated here.

[0024] Preferably, the Meconopsis is further dried before being mixed with the solution containing a deep eutectic solvent.

[0025] Preferably, the drying temperature is 40-60℃ and the time is 2-5 hours.

[0026] The temperature can be selected from 40℃, 42℃, 44℃, 46℃, 48℃, 50℃, 52℃, 54℃, 56℃, 58℃, 60℃, etc., and the time can be selected from 2h, 2.2h, 2.5h, 2.8h, 3h, 3.2h, 3.5h, 3.8h, 4h, 4.2h, 4.5h, 4.8h, 5h, etc. Other specific point values ​​within the above range can be selected, which will not be elaborated here.

[0027] Preferably, the ratio of Meconopsis to the solution containing the deep eutectic solvent is 1g:(10-50)mL, where the specific values ​​in (10-50) can be selected from 10, 12, 15, 18, 20, 22, 25, 28, 30, 32, 35, 38, 40, 42, 45, 48, 50, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0028] Preferably, the mixing temperature is 30-70℃, such as 30℃, 35℃, 40℃, 45℃, 50℃, 55℃, 60℃, 65℃, 70℃, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0029] Preferably, the power of the ultrasound is 200-500W and the duration is 0.5-3h.

[0030] The power can be selected from 200W, 250W, 300W, 350W, 400W, 450W, 500W, etc., and the time can be selected from 0.5h, 1h, 1.5h, 2h, 2.5h, 3h, etc. Other specific values ​​within the above range can be selected, which will not be elaborated here.

[0031] Preferably, the centrifugation speed is 5000-10000 r / min and the time is 5-20 min.

[0032] The rotational speed can be selected from 5000 r / min, 5500 r / min, 6000 r / min, 6500 r / min, 7000 r / min, 7500 r / min, 8000 r / min, 8500 r / min, 9000 r / min, 9500 r / min, 10000 r / min, etc., and the time can be 5 min, 6 min, 7 min, 8 min, 9 min, 10 min, 11 min, 12 min, 13 min, 14 min, 15 min, 16 min, 17 min, 18 min, 19 min, 20 min, etc. Other specific values ​​within the above range can be selected, which will not be elaborated here.

[0033] In a second aspect, the present invention provides a quercetin extract prepared by the method for extracting quercetin from Meconopsis as described in the first aspect.

[0034] Thirdly, the present invention provides the application of the quercetin extract according to the second aspect in cosmetics.

[0035] Preferably, the amount of quercetin extract added to the cosmetic is 0.01%-1%, such as 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.15%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] Matrine propylene glycol (NaDES) solvent boasts 100% atom economy, is environmentally friendly, and exhibits good biocompatibility, making it suitable as an extraction solvent for quercetin from *Meconopsis*. By coupling with ultrasound enhancement, the quercetin content in the extract can be effectively increased. Furthermore, as a solvent, matrine propylene glycol (NaDES) eliminates the need for separation and can be used as an active ingredient in skincare products, synergistically enhancing quercetin's antibacterial, anti-inflammatory, and anti-wrinkle effects. Attached Figure Description

[0038] Figure 1 The results show the extraction rates of quercetin from Meconopsis using different solvents.

[0039] Figure 2 The images show SEM analyses of *Meconopsis* before and after extraction with different solvents. Figure A shows untreated *Meconopsis*, Figure B shows *Meconopsis* treated with water extraction, Figure C shows *Meconopsis* treated with 70 wt% methanol extraction, Figure D shows *Meconopsis* treated with 70 wt% ethanol extraction, and Figure E shows *Meconopsis* treated with the eutectic solvent described in Preparation Example 1.

[0040] Figure 3 The DPPH scavenging rate of Meconopsis extract treated with different solvents is shown. Detailed Implementation

[0041] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention in any way.

[0042] Preparation Example 1

[0043] This preparation example provides a deep eutectic solvent, which is obtained by reacting matrine and propylene glycol in a molar ratio of 1:3.

[0044] The preparation method is as follows: matrine and propylene glycol are stirred and heated at 50°C for 3 hours to obtain the product.

[0045] Preparation Example 2

[0046] This preparation example provides a deep eutectic solvent, which is obtained by reacting matrine and propylene glycol in a molar ratio of 1:2.

[0047] The preparation method is as follows: matrine and propylene glycol are stirred and heated at 30°C for 8 hours to obtain the product.

[0048] Preparation Example 3

[0049] This preparation example provides a deep eutectic solvent, which is obtained by reacting matrine and propylene glycol in a molar ratio of 1:1.

[0050] The preparation method is as follows: matrine and propylene glycol are stirred and heated at 60°C for 1 hour to obtain the product.

[0051] Preparation Example 4

[0052] This preparation example provides a deep eutectic solvent, which is obtained by reacting betaine and propylene glycol in a molar ratio of 1:1.

[0053] The preparation method is the same as in Preparation Example 1.

[0054] Preparation Example 5

[0055] This preparation example provides a deep eutectic solvent, which is obtained by reacting sorbitol and betaine in a molar ratio of 1:1.

[0056] The preparation method is the same as in Preparation Example 1.

[0057] Preparation Example 6

[0058] This preparation example provides a deep eutectic solvent, which is obtained by reacting betaine and citric acid in a molar ratio of 1:1.

[0059] The preparation method is the same as in Preparation Example 1.

[0060] Preparation Example 7

[0061] This preparation example provides a deep eutectic solvent, which is obtained by reacting L-carnitine and citric acid in a molar ratio of 1:1.

[0062] The preparation method is the same as in Preparation Example 1.

[0063] Example 1

[0064] This embodiment provides a method for extracting quercetin from Meconopsis, the method comprising:

[0065] (1) Wash the Meconopsis with clean water, dry it at a low temperature of 45℃ for 8 hours, crush it, and sieve it through a 60-mesh screen.

[0066] (2) The eutectic solvent described in Preparation Example 1 was mixed with ultrapure water until the mass percentage of the eutectic solvent was 70%, and then mixed with Meconopsis. The weight-volume ratio of Meconopsis to the solution containing the eutectic solvent was 1 g: 25 mL. The mixture was then subjected to enhanced treatment at 70 °C and ultrasonic power of 400 W for 1 h to obtain the extract.

[0067] (3) Centrifuge the extract to obtain the supernatant. The centrifugation parameters are 10000 r / min and 15 min. Filter the supernatant to obtain the extract. Use a 0.45 μm organic phase filter membrane and concentrate to obtain the final extract.

[0068] Example 2

[0069] This embodiment provides a method for extracting quercetin from Meconopsis, the method comprising:

[0070] (1) Wash the Meconopsis with clean water, dry it at a low temperature of 40℃ for 6 hours, crush it, and sieve it through a 60-mesh screen.

[0071] (2) The eutectic solvent described in Preparation Example 1 was mixed with ultrapure water until the mass percentage of the eutectic solvent was 20%, and then mixed with Meconopsis. The weight-volume ratio of Meconopsis to the solution containing the eutectic solvent was 1 g: 50 mL. The mixture was then subjected to enhanced treatment at 70 °C and ultrasonic power of 500 W for 3 h to obtain the extract.

[0072] (3) Centrifuge the extract to obtain the supernatant. The centrifugation parameters are 8000 r / min and 10 min. Filter the supernatant to obtain the extract. Use a 0.45 μm organic phase filter membrane and concentrate to obtain the final extract.

[0073] Example 3

[0074] This embodiment provides a method for extracting quercetin from Meconopsis, the method comprising:

[0075] (1) Wash the Meconopsis with clean water, dry it at 60℃ for 2 hours, crush it, and sieve it through a 60-mesh screen.

[0076] (2) The eutectic solvent described in Preparation Example 1 was mixed with ultrapure water until the mass percentage of the eutectic solvent was 80%, and then mixed with Meconopsis. The weight-volume ratio of Meconopsis to the solution containing the eutectic solvent was 1 g: 10 mL. The mixture was then subjected to enhanced treatment at 30 °C and ultrasonic power of 200 W for 0.5 h to obtain the extract.

[0077] (3) Centrifuge the extract to obtain the supernatant. The centrifugation parameters are 10000 r / min and 5 min. Filter the supernatant to obtain the extract. Use a 0.45 μm organic phase filter membrane and concentrate to obtain the final extract.

[0078] Example 4

[0079] This embodiment provides a method for extracting quercetin from Meconopsis, which differs from Example 1 only in that step (2) is: "The deep eutectic solvent described in Preparation Example 2 is mixed with ultrapure water until the mass percentage of the deep eutectic solvent is 70%, and then mixed with Meconopsis. The weight-volume ratio of Meconopsis to the solution containing the deep eutectic solvent is 1g:25mL. The mixture is then subjected to enhanced treatment at 70°C and ultrasonic power of 400W for 1 hour to obtain the extract."

[0080] Other operations remain unchanged.

[0081] Example 5

[0082] This embodiment provides a method for extracting quercetin from Meconopsis, which differs from Example 1 only in that step (2) is: "The eutectic solvent described in Preparation Example 3 is mixed with ultrapure water until the mass percentage of the eutectic solvent is 70%, and then mixed with Meconopsis. The weight-volume ratio of Meconopsis to the solution containing the eutectic solvent is 1g:25mL. The mixture is then subjected to enhanced treatment at 70°C and 400W ultrasonic power for 1h to obtain the extract."

[0083] Other operations remain unchanged.

[0084] Example 6

[0085] This embodiment provides a method for extracting quercetin from Meconopsis, which differs from Example 1 only in that step (2) is: "The eutectic solvent described in Preparation Example 4 is mixed with ultrapure water until the mass percentage of the eutectic solvent is 70%, and then mixed with Meconopsis. The weight-volume ratio of Meconopsis to the solution containing the eutectic solvent is 1g:25mL. The mixture is then subjected to enhanced treatment at 70°C and ultrasonic power of 400W for 1 hour to obtain the extract."

[0086] Other operations remain unchanged.

[0087] Example 7

[0088] This embodiment provides a method for extracting quercetin from Meconopsis, which differs from Example 1 only in that step (2) is: "The deep eutectic solvent described in Preparation Example 5 is mixed with ultrapure water until the mass percentage of the deep eutectic solvent is 70%, and then mixed with Meconopsis. The weight-volume ratio of Meconopsis to the solution containing the deep eutectic solvent is 1g:25mL. The mixture is then subjected to enhanced treatment at 70°C and ultrasonic power of 400W for 1h to obtain the extract."

[0089] Other operations remain unchanged.

[0090] Example 8

[0091] This embodiment provides a method for extracting quercetin from Meconopsis, which differs from Example 1 only in that step (2) is: "The deep eutectic solvent described in Preparation Example 6 is mixed with ultrapure water until the mass percentage of the deep eutectic solvent is 70%, and then mixed with Meconopsis. The weight-volume ratio of Meconopsis to the solution containing the deep eutectic solvent is 1g:25mL. The mixture is then subjected to enhanced treatment at 70°C and ultrasonic power of 400W for 1h to obtain the extract."

[0092] Other operations remain unchanged.

[0093] Example 9

[0094] This embodiment provides a method for extracting quercetin from Meconopsis, which differs from Example 1 only in that step (2) is: "The deep eutectic solvent described in Preparation Example 7 is mixed with ultrapure water until the mass percentage of the deep eutectic solvent is 70%, and then mixed with Meconopsis. The weight-volume ratio of Meconopsis to the solution containing the deep eutectic solvent is 1g:25mL. The mixture is then subjected to enhanced treatment at 70°C and ultrasonic power of 400W for 1h to obtain the extract."

[0095] Other operations remain unchanged.

[0096] Comparative Example 1

[0097] This comparative example provides a method for extracting quercetin from Meconopsis. The only difference between this method and Example 1 is that step (2) is to "mix ultrapure water with Meconopsis, with a ratio of 1g:25mL between Meconopsis and ultrapure water, and perform enhanced treatment at 70°C and 400W ultrasonic power for 1h to obtain the extract".

[0098] Other operations remain unchanged.

[0099] Comparative Example 2

[0100] This comparative example provides a method for extracting quercetin from Meconopsis. The only difference between this method and Example 1 is that step (2) is: “mixing 70wt% methanol aqueous solution with Meconopsis, the ratio of Meconopsis to 70wt% methanol aqueous solution is 1g:25mL, and strengthening the treatment at 70℃ and ultrasonic power 400W for 1h to obtain the extract.”

[0101] Other operations remain unchanged.

[0102] Comparative Example 3

[0103] This comparative example provides a method for extracting quercetin from Meconopsis. The only difference between this method and Example 1 is that step (2) is: “mix 70wt% ethanol aqueous solution with Meconopsis, the ratio of Meconopsis to 70wt% ethanol aqueous solution is 1g:25mL, and strengthen the treatment at 70℃ and ultrasonic power 400W for 1h to obtain the extract.”

[0104] Other operations remain unchanged.

[0105] Test Example 1

[0106] Quercetin content determination

[0107] Test method:

[0108] Extracts of *Meconopsis* were prepared by deep eutectic solvent extraction using different preparation methods. The extracts were then analyzed by HPLC (high performance liquid chromatography). The content of quercetin in the *Meconopsis* extracts was calculated by referring to the standard curve of the quercetin standard.

[0109] The results are as follows Figure 1 As shown, by Figure 1 It can be seen that, compared with the 70wt% matrine propylene glycol (1:3) NaDES solvent (Example 1), the extraction yield of quercetin from Meconopsis was lower with traditional solvents such as water (Comparative Example 1), 70wt% methanol-water (Comparative Example 2), and 70wt% ethanol-water (Comparative Example 3). Specifically, the extraction rate of quercetin from Meconopsis using the 70wt% matrine propylene glycol (1:3) NaDES solvent (Example 1) was 0.541 mg / g, which is 6.47 times that of water (Comparative Example 1), 1.71 times that of 70wt% methanol-water (Comparative Example 2), and 2.14 times that of 70wt% ethanol-water (Comparative Example 3). In addition, the effects of different molar ratios and different types of NaDES solutions on the extraction of quercetin from Meconopsis were tested. The results showed that, within the test range, 70% matrine propylene glycol (1:3) NaDES (Example 1) had the highest extraction rate of quercetin from Meconopsis, indicating that among different NaDES solutions, 70% matrine propylene glycol (1:3) NaDES (Example 1) has a specific advantage in the extraction of quercetin from Meconopsis.

[0110] Test Example 2

[0111] SEM analysis of Meconopsis raw material before and after treatment with different solvents

[0112] The microstructure of *Meconopsis* raw material treated with the optimal 70wt% matrine-propylene glycol (1:3) NaDES (Example 1) after conventional solvent extraction and screening was observed using scanning electron microscopy. The results showed that the untreated *Meconopsis* raw material was in relatively large and intact flakes. The *Meconopsis* raw material treated with water, methanol-water, and ethanol-water (Comparative Examples 1-3) underwent some slight changes in microstructure, and the flake structure was destroyed, resulting in the stacking of small flake structures. The surface tissue of the *Meconopsis* raw material treated with 70% matrine-propylene glycol (1:3) NaDES (Example 1) was significantly destroyed, presenting a multi-layered stack of smooth flakes. The surface morphology changed the most compared to the untreated raw material. This indicates that the 70% matrine-propylene glycol (1:3) NaDES (Example 1) extraction process can effectively destroy the surface structure of plant raw materials and promote the dissolution of active ingredients. This demonstrates its mechanism of promoting the extraction of quercetin from *Meconopsis*, resulting in higher extraction efficiency of quercetin and thus higher yield of active ingredients.

[0113] Test Example 3

[0114] DPPH free radical scavenging test

[0115] Test method:

[0116] Prepare DPPH solutions with a concentration of 0.12 mg / mL using anhydrous ethanol. Dilute the sample solution to be tested with ethanol to a concentration of 1%. Take 1 mL of the diluted sample and mix it thoroughly with 2 mL of anhydrous ethanol and 1 mL of DPPH solution. Let it stand in the dark for 5 min, and then measure the absorbance value at a wavelength of 517 nm.

[0117]

[0118] A0 is the absorbance value of anhydrous ethanol replacing the sample solution; As is the absorbance value of the sample solution after reacting with DPPH free radicals; Ab is the absorbance of anhydrous ethanol replacing the DPPH solution.

[0119] Results and Figure 3 As shown, the extract of *Meconopsis* treated with 70% matrine propylene glycol (1:3) NaDES (Example 1) exhibited the highest DPPH radical scavenging rate, reaching 80.76%. The results indicate that the DPPH radical scavenging rates of *Meconopsis* extracts obtained with different molar ratios of the same type of NaDES (70wt% matrine propylene glycol NaDES) were similar, while the extracts obtained with different types of NaDES showed some differences. This further suggests that 70% matrine propylene glycol NaDES has an advantage in extracting quercetin from *Meconopsis*, thus the extract possesses higher antioxidant properties.

[0120] Test Example 4

[0121] Anti-wrinkle efficacy test

[0122] This test consists of two parts. The first part is based on fibroblasts, which involves cytotoxicity assays to determine the drug concentration of the sample on the fibroblasts. The second part involves detecting changes in HES1 gene expression levels after UVA irradiation of fibroblasts.

[0123] First, the sample concentration was determined to be 0.1563% through cytotoxicity testing. Then, blank group (BC), negative control group (NC), positive control group (PC, 15.6 ppm quercetin) and sample group (0.1563% Meconopsis extract) were set up. Fibroblasts incubated at 37℃ / 5% CO2 were administered the drug. According to the grouping, except for BC, the groups were irradiated with UVA. After culturing for 24 h, q-PCR was performed to analyze the HES1 gene expression level.

[0124] Table 1

[0125] Sample Name Relative expression level average SD P-value Upward adjustment rate BC 1.00 0.12 / / NC 0.75 0.03 0.027# / PC 2.14 0.21 0.000** 185.33％ Example 1 1.18 0.06 0.000** 57.33％

[0126] Note: Significance compared to group BC is indicated by *, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by **.

[0127] Based on fibroblasts, compared with the control group, the HES1 gene expression level of the sample Meconopsis extract was significantly upregulated at a concentration of 0.1563% (v / v), with an upregulation rate of 57.33%, indicating that the sample can exert anti-wrinkle effects by upregulating the HES1 gene expression level at the corresponding concentration.

[0128] The applicant declares that this invention illustrates a method for extracting quercetin from Meconopsis, its products, and applications through the above embodiments. However, this invention is not limited to the above embodiments, meaning that this invention does not necessarily rely on the above embodiments for implementation. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, additions of auxiliary components, and selection of specific methods all fall within the protection and disclosure scope of this invention.

[0129] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0130] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A method for extracting quercetin from Meconopsis, characterized in that, The method includes: (1) Mix Meconopsis with a solution containing a deep eutectic solvent and sonicate to obtain an extract; (2) Centrifuge the extract, filter the supernatant, and concentrate it to obtain the final product; The deep eutectic solvent is obtained by reacting a hydrogen bond donor and a hydrogen bond acceptor; The hydrogen bond donor is propylene glycol; the hydrogen bond acceptor is matrine. The molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:(1-3); The mass percentage of the eutectic solvent in the solution containing the eutectic solvent is 20-80%. The reaction is carried out at a temperature of 30-60℃ for 1-8 hours. The ratio of Meconopsis to a solution containing a deep eutectic solvent is 1 g:(10-50) mL.

2. The method according to claim 1, characterized in that, The Meconopsis is further dried before being mixed with a solution containing a deep eutectic solvent.

3. The method according to claim 2, characterized in that, The drying temperature is 40-60℃, and the time is 2-5 hours.

4. The method according to claim 1, characterized in that, The mixing temperature is 30-70℃.

5. The method according to claim 1, characterized in that, The ultrasound power is 200-500W, and the duration is 0.5-3h.

6. The method according to claim 1, characterized in that, The centrifugation speed is 5000-10000 r / min, and the time is 5-20 min.

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