A multi-target detection device suitable for microfluidic chip and detection method thereof

Abstract

The present invention belongs to the field of reagent detection technology, and specifically relates to a multi-target detection device and a detection method suitable for a microfluidic chip. The multi-target detection device includes: a heating component that controls the temperature inside the microfluidic chip component, and amplifies the test agent in the microfluidic chip component; a reaction mixing component that intermittently attracts magnetic beads in the microfluidic chip component to move through magnetic force, and mixes the test agent in the microfluidic chip component with a fluorescent labeled reagent; a fluorescent detection component that emits detection light to the microfluidic chip component, and outputs corresponding detection results through an observation component; the present invention can quickly centrifuge and heat amplify the test agent through the non-contact arrangement of the heating component and the microfluidic chip component, and the reaction mixing component can evenly mix the test agent and the fluorescent labeled reagent, and at the same time cooperate with the fluorescent detection component and the observation component to realize rapid multi-target detection and automation of the detection process. It has a small size, low cost, simple structure, convenient operation and is easy to carry.

Description

Technical Field

[0001] The present invention belongs to the technical field of reagent detection, and in particular relates to a multi-target detection device suitable for a microfluidic chip and a detection method thereof. Background Art

[0002] The microfluidic chip uses centrifugal force as the fluid driving force to achieve detection and analysis of multiple indicators of the same sample to be tested.

[0003] Currently, the microfluidic chips in multi-target detection devices need to use contact heating to achieve amplification, and the test agents in the microfluidic chip need to enter the reaction chamber under the action of centrifugation. The various functional modules are relatively scattered, resulting in a long entire detection cycle. In addition, after the test agents in the microfluidic chip are amplified, they need to be taken out and placed in a test tube to mix with the fluorescent labeling reagent. Insufficient mixing will affect the detection accuracy, and the entire detection process is relatively complicated.

[0004] Therefore, there is an urgent need to develop a new multi-target detection device and detection method suitable for microfluidic chips to solve the above problems. Summary of the Invention

[0005] The purpose of the present invention is to provide a multi-target detection device and a detection method suitable for a microfluidic chip.

[0006] In order to solve the above technical problems, the present invention provides a multi-target detection device suitable for a microfluidic chip, which includes: a shell component, a heating component, a centrifugal component, a fluorescence detection component, a microfluidic chip component, a reaction mixing component and an observation component; wherein the heating component, the centrifugal component, the fluorescence detection component, the microfluidic chip component, and the reaction mixing component are installed in the shell component, the heating component, the centrifugal component, and the fluorescence detection component are located below the microfluidic chip component, the centrifugal component is connected to the microfluidic chip component, and the reaction mixing component is located above the microfluidic chip component; the observation component is installed in the shell The outside of the component, and the observation component is located in the optical path of the fluorescence detection component; the centrifugal component drives the microfluidic chip component to rotate to centrifuge the test agent in the microfluidic chip component to the corresponding position; the heating component controls the temperature in the microfluidic chip component to amplify the test agent in the microfluidic chip component; the reaction mixing component intermittently attracts the magnetic beads in the microfluidic chip component to move through magnetic force to mix the test agent in the microfluidic chip component with the fluorescent labeling reagent; and the fluorescence detection component emits detection light to the microfluidic chip component, and outputs the corresponding detection result through the observation component.

[0007] Specifically, the shell assembly includes: a main shell and a shell cover; the shell cover is hinged to the top of the main shell; a groove 1 is provided on the top of the main shell for loading the heating component, centrifugal component, fluorescence detection component, and microfluidic chip component; a groove 2 is provided on the shell cover for loading the reaction mixing component; the observation component is movably mounted on the shell cover, and a window 1 connecting to the groove 2 is provided on the shell cover.

[0008] Specifically, the microfluidic chip assembly includes: a microfluidic chip cover plate, a microfluidic chip middle layer, and a microfluidic chip base plate; the microfluidic chip cover plate, the microfluidic chip middle layer, and the microfluidic chip base plate are stacked in sequence from top to bottom; a sample addition hole is provided on the microfluidic chip cover plate, and a detection hole is provided on the microfluidic chip middle layer; the corresponding detection holes on the microfluidic chip cover plate and the microfluidic chip base plate are solidly arranged, and the sample addition hole is connected to the detection hole.

[0009] Specifically, the microfluidic chip cover is provided with air holes, which are connected to the detection holes.

[0010] Specifically, the detection hole is divided into several sub-holes; the sub-holes include a quantitative hole, a first connecting hole, a buffer hole, a second connecting hole and a reaction hole; the quantitative hole, the first connecting hole, the buffer hole, the second connecting hole and the reaction hole respectively form a quantitative cavity, a first channel, a buffer cavity, a second channel and a reaction cavity with the microfluidic chip cover plate and the microfluidic chip bottom plate; any of the quantitative cavities is connected to the adjacent quantitative cavity through the second channel, and the quantitative cavity is connected to the reaction cavity through the first channel and the buffer cavity.

[0011] Specifically, the reaction mixing assembly includes: a mixing drive, a fixing frame, a plurality of mixing magnets and a plurality of magnetic beads; the mixing drive is located in slot two, and the mixing drive is connected to the shell cover; the fixing frame is connected to the mixing drive, and each of the mixing magnets is installed on the fixing frame; each of the magnetic beads is respectively located in a corresponding reaction chamber.

[0012] Specifically, the fluorescence detection component includes: an LED light output substrate, a light homogenizing plate, a filter 1, and a filter 2; the LED light output substrate, the light homogenizing plate, and the filter 1 are arranged in sequence from bottom to top below the microfluidic chip component, the filter 2 is installed in window 1, and the microfluidic chip component is located between filter 1 and filter 2.

[0013] Specifically, the observation assembly includes: a magnifying glass; the magnifying glass is installed on the housing assembly, and the observation direction of the magnifying glass is set toward the second filter.

[0014] Specifically, the magnifying glass is movably connected to the housing assembly via a bracket and a damping shaft.

[0015] On the other hand, the present invention provides a detection method using the multi-target detection device suitable for a microfluidic chip as described above, which includes: driving the microfluidic chip assembly to rotate by a centrifugal assembly to centrifuge the test agent in the microfluidic chip assembly to a corresponding position; controlling the temperature in the microfluidic chip assembly by a heating assembly to amplify the test agent in the microfluidic chip assembly; using a reaction mixing assembly to intermittently attract magnetic beads in the microfluidic chip assembly by magnetic force to move, so that the test agent in the microfluidic chip assembly is mixed with a fluorescent labeled reagent; emitting detection light to the microfluidic chip assembly by a fluorescent detection assembly, and outputting corresponding detection results through an observation assembly.

[0016] The beneficial effect of the present invention is that the present invention can quickly centrifuge and heat-amplify the test agent through the non-contact setting of the heating component and the microfluidic chip component, and the reaction mixing component can evenly mix the test agent and the fluorescent labeling reagent. At the same time, it cooperates with the fluorescent detection component and the observation component to realize rapid multi-target detection and automation of the detection process. It is small in size, low in cost, simple in structure, and easy to operate and carry.

[0017] Other features and advantages of the present invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the present invention.

[0018] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0020] Figure 1 It is a perspective view of the right front side of the multi-target detection device of the present invention; Figure 2 It is a rear view of the multi-target detection device of the present invention; Figure 3 is a structural stereogram of the microfluidic chip assembly of the present invention; Figure 4 A top view of the microfluidic chip cover of the present invention; Figure 5 A top view of the intermediate layer of the microfluidic chip of the present invention; Figure 6 A top view of the microfluidic chip base plate of the present invention; Figure 7 A perspective view of the left front side of the heating assembly of the present invention; Figure 8 A perspective view of the left front side of the fluorescence detection assembly of the present invention; Figure 9is a front view of the microfluidic chip assembly of the present invention; Figure 10 A perspective view of the right front side of the housing cover of the present invention; Figure 11 A perspective view of the right front side of the interior of the main housing of the present invention; Figure 12 A front view of the interior of the main housing of the present invention; Figure 13 A top view of the microfluidic chip assembly of the present invention; Figure 14 A perspective view of the interior of the housing cover and the right front side of the hybrid drive unit of the present invention; Figure 15 A perspective view of the right front side of the housing cover of the present invention; Figure 16 A front view of the interior of the housing cover of the present invention; Figure 17 A top view of the main housing of the present invention; Figure 18 A front view of the interior of the main housing of the present invention; Figure 19 A top view of the main control board of the present invention; Figure 20 It is a front view of the main control board of the present invention; Figure 21 is a front view of the bottom mounting plate of the present invention; Figure 22 A right front perspective view of the battery compartment housing and its interior of the present invention; Figure 23 A perspective view of the right front side of the heating assembly of the present invention; Figure 24 It is a top view of the heating area of ​​the present invention.

[0021] In the picture:

[0022] 1. Sample loading hole; 2. Bonding positioning hole; 3. Air hole; 4. Insulation groove; 5. Second channel; 6. Fixing hole 1; 7. Buffer chamber; 8. Quantitative chamber; 9. First channel; 10. Reaction chamber; 11. Microfluidic chip cover; 12. Microfluidic chip middle layer; 13. Microfluidic chip bottom plate; 14. Positioning hole 1; 15. Shell cover; 16. Main shell; 17. Bottom mounting plate; 18. Battery compartment shell; 19. Battery compartment bottom plate; 20. Power switch 21. Type-C power interface; 22. Transparent PMMA protective shell; 23. Magnifying glass; 24. Bracket; 25. Rotating hinge; 26. Fixed hinge; 27. Filter 1; 28. Damping shaft; 29. ​​Filter 2; 30. Fixing frame; 31. Hybrid magnet; 32. Hybrid drive unit; 33. Hall sensor; 34. PCB heating circuit board; 35. Core seat body; 36. Core seat bottom plate; 37. Heating expansion Increase button; 38, button indicator circuit board; 39, main control board; 40, light uniformity board; 41, centrifugal motor; 42, harness hole 1; 43, LED light output substrate; 44, shaft sleeve; 45, detection position magnet; 46, harness hole 2; 47, mounting hole 1; 48, heating position magnet; 49, closing magnet 1; 50, fixing hole 2; 51, closing magnet 2; 52, lithium battery module; 53, mounting hole 2; 54, fixing hole 3; 55, installation Mounting slot; 56, wiring harness hole three; 57, support column; 58, fixing hole four; 59, shaft hole; 60, wiring harness hole four; 61, bottom plate magnet; 62, two-color LED indicator light; 63, single-color LED indicator light; 64, button cap; 65, fluorescence detection button; 66, battery compartment magnet; 67, microfluidic chip magnet; 68, microfluidic chip assembly; 69, heating resistor; 70, temperature measuring chip; 71, terminal; 72, fixing hole five. DETAILED DESCRIPTION

[0023] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of them. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0024] Example 1, in this embodiment, as Figures 1 to 24As shown, this embodiment provides a multi-target detection device suitable for a microfluidic chip, which includes: a housing assembly, a heating assembly, a centrifugal assembly, a fluorescence detection assembly, a microfluidic chip assembly 68, a reaction mixing assembly and an observation assembly; wherein the heating assembly, the centrifugal assembly, the fluorescence detection assembly, the microfluidic chip assembly 68, and the reaction mixing assembly are installed in the housing assembly, the heating assembly, the centrifugal assembly, and the fluorescence detection assembly are located below the microfluidic chip assembly 68, the centrifugal assembly is connected to the microfluidic chip assembly 68, and the reaction mixing assembly is located above the microfluidic chip assembly 68; the observation assembly is installed on the outside of the housing assembly , and the observation component is located in the optical path of the fluorescence detection component; the centrifugal component drives the microfluidic chip component 68 to rotate to centrifuge the test agent in the microfluidic chip component 68 to the corresponding position; the heating component controls the temperature in the microfluidic chip component 68 to amplify the test agent in the microfluidic chip component 68; the reaction mixing component intermittently attracts the magnetic beads in the microfluidic chip component 68 through magnetic force to move, so that the test agent in the microfluidic chip component 68 is mixed with the fluorescent labeling reagent; and the fluorescence detection component emits detection light to the microfluidic chip component 68, and outputs the corresponding detection result through the observation component.

[0025] In this embodiment, the shell assembly includes: a main shell 16 and a shell cover 15; the shell cover 15 is hinged to the top of the main shell 16; a groove 1 is provided on the top of the main shell 16 for loading the heating component, centrifugal component, fluorescence detection component, and microfluidic chip component 68; a groove 2 is provided on the shell cover 15 for loading the reaction mixing component; the observation component is movably mounted on the shell cover 15, and a window 1 connecting to the groove 2 is provided on the shell cover 15.

[0026] In this embodiment, the main shell 16 and the shell cover 15 are both made of black PLA engineering plastic and processed by 3D printing, which makes the device lightweight, easy to carry, and has certain heat preservation properties. At the same time, the black material can reduce the interference of the environment on the visual fluorescence detection.

[0027] In this embodiment, the centrifugal assembly includes a centrifugal motor 41, which is fixed to the core seat body 35 through the fixing hole 58. Its rotating parts and the shaft sleeve 44 extend out of the surface of the core seat body 35 through the shaft hole 59. The main control board 39 controls the output power of the centrifugal motor 41. At the same time, the centrifugal and position control functions are realized by detecting the cooperation between the position magnet 45 and the Hall sensor 33.

[0028] In this embodiment, the microfluidic chip assembly 68 includes: a microfluidic chip cover plate 11, a microfluidic chip middle layer 12, and a microfluidic chip base plate 13; the microfluidic chip cover plate 11, the microfluidic chip middle layer 12, and the microfluidic chip base plate 13 are stacked in sequence from top to bottom; a sample addition hole 1 is provided on the microfluidic chip cover plate 11, and a detection hole is provided on the microfluidic chip middle layer 12; the corresponding detection holes on the microfluidic chip cover plate 11 and the microfluidic chip base plate 13 are solidly arranged, and the sample addition hole 1 is connected to the detection hole.

[0029] In this embodiment, the microfluidic chip cover 11, the microfluidic chip middle layer 12, and the microfluidic chip base plate 13 are arranged axially symmetrically as a whole. At the same time, the microfluidic chip cover 11 and the microfluidic chip base plate 13 clamp the microfluidic chip middle layer 12 to form multiple groups of detection units with the same structure, which can realize rapid multi-index joint detection for the same sample to be tested.

[0030] In this embodiment, the microfluidic chip cover plate 11, the microfluidic chip middle layer 12, and the microfluidic chip base plate 13 are all provided with bonding positioning holes 2. The microfluidic chip cover plate 11, the microfluidic chip middle layer 12, and the microfluidic chip base plate 13 are bonded and fixed by double-sided adhesive, which can ensure the accuracy of the microfluidic chip assembly 68.

[0031] In this embodiment, an air hole 3 is provided on the microfluidic chip cover 11, and the air hole 3 is connected to the detection hole. The air hole 3 connects the detection hole and the external atmospheric pressure, so that the sample addition is smooth. For multiple detection units, the microfluidic chip only needs to be injected once, and at the same time, only the sample addition hole 1 and the air hole 3 need to be closed. The operation process is simple and easy to use.

[0032] In this embodiment, the detection hole is divided into several sub-holes; the sub-holes include a quantitative hole, a first connecting hole, a buffer hole, a second connecting hole and a reaction hole; the quantitative hole, the first connecting hole, the buffer hole, the second connecting hole and the reaction hole respectively form a quantitative cavity 8, a first channel 9, a buffer cavity 7, a second channel 5 and a reaction cavity 10 with the microfluidic chip cover plate 11 and the microfluidic chip bottom plate 13; any of the quantitative cavities 8 is connected to the adjacent quantitative cavity 8 through the second channel 5, and the quantitative cavity 8 is connected to the reaction cavity 10 through the first channel 9 and the buffer cavity 7.

[0033] In this embodiment, the centrifugal motor 41, the Hall sensor 33, the main control board 39, the heating position magnet 48, the detection position magnet 45 and the microfluidic chip magnet 67 cooperate with each other to realize position control. The specific process is as follows: the Hall sensor 33 can detect the microfluidic chip magnet 67. After detecting the signal, the main control board 39 will adjust the speed of the centrifugal motor 41 according to this signal. The control process is as follows: through the output of the PWM duty cycle signal of the main control board 39, the power supply voltage of the centrifugal motor 41 is adjusted, thereby realizing the adjustment of the motor speed, so that the microfluidic chip assembly 68 rotates slowly until the microfluidic chip magnet 67 is adsorbed with the heating position magnet 48. At this time, the reaction chamber 10 of each detection unit in the microfluidic chip assembly 68 is located directly above each heating area of ​​the PCB heating circuit board 34, and heating amplification can be started at this time; similarly, the detection position magnet 45 is responsible for rotating the reaction chamber 10 of each detection unit to a fixed angle to the detection position for fluorescence detection.

[0034] Specifically, the microfluidic chip cover plate 11, the microfluidic chip middle layer 12, and the microfluidic chip bottom plate 13 are disk-shaped structures as a whole, preferably with a radius of 26 mm and a height of 4 mm; each detection unit is located in the radial direction of the circular structure.

[0035] In this embodiment, support columns 57 on the surface of the core base body 35 secure the PCB heating circuit board 34 and simultaneously elevate it, removing it from contact with the core base body 35 surface, thereby reducing heat transfer. Furthermore, each of the three-layer detection unit reaction chambers 10 in the microfluidic chip assembly 68 is provided with thermal insulation grooves 4 on both sides, effectively isolating the surrounding heat dissipation and improving heating efficiency. The PCB heating circuit board 34 is annular in shape with a notch (approximately 47 degrees) designed to allow for visual fluorescence detection, allowing the different reaction chambers 10 in the microfluidic chip assembly 68 to rotate into the detection position and initiate visual fluorescence detection.

[0036] In this embodiment, the reaction mixing assembly includes: a mixing driver 32, a fixing frame 30, a plurality of mixing magnets 31 and a plurality of magnetic beads; the mixing driver 32 is located in slot 2 and is connected to the housing cover 15; the fixing frame 30 is connected to the mixing driver 32, and each of the mixing magnets 31 is mounted on the fixing frame 30; and each of the magnetic beads is located in a corresponding reaction chamber 10.

[0037] In this embodiment, the hybrid drive element 32 adopts a small DC hollow cup motor.

[0038] Specifically, the rotation speed of the mixing driver 32 is adjusted by setting the PWM output duty cycle of the main control board 39 , and a more appropriate rotation speed is determined to achieve mixing of magnetic beads in the reaction chamber 10 in the microfluidic chip assembly 68 .

[0039] In this embodiment, after the shell cover 15 is closed, the reaction mixing assembly is parallel to the microfluidic chip assembly 68, the mixing magnet 31 is located directly above the reaction chamber 10 and is non-contacting, and the spacing between the mixing magnet 31 and the microfluidic chip cover 11 is 0.5 mm. When the mixing drive 32 rotates, the magnetic adsorption effect can mix the magnetic beads inside the reaction chamber 10.

[0040] In this embodiment, the fluorescence detection component includes: an LED light output substrate 43, a light homogenizing plate 40, a filter 27, and a filter 29; the LED light output substrate 43, the light homogenizing plate 40, and the filter 27 are arranged from bottom to top below the microfluidic chip component 68, the filter 2 29 is installed in window 1, and the microfluidic chip component 68 is located between the filter 1 27 and the filter 2 29.

[0041] In this embodiment, the observation assembly includes: a magnifying glass 23 ; the magnifying glass 23 is mounted on the housing assembly, and the observation direction of the magnifying glass 23 is set toward the second filter 29 .

[0042] In this embodiment, the magnifying glass 23 is movably connected to the housing assembly via a bracket 24 and a damping shaft 28 .

[0043] In this embodiment, a transparent PMMA protective shell 22 is also installed in window 1. The LED light-emitting substrate 43, the light-dispersing plate 40, the filter 1 27, the filter 2 29, the transparent PMMA protective shell 22, and the magnifying glass 23 form a vertical light path. The specific light path is as follows: the LED light-emitting substrate 43 is the excitation light with a central wavelength of 452 nm; then it enters the light-dispersing plate 40 vertically, turning the LED point light source into a surface light source, and the wavelength (type) of the light does not change; then it enters the filter 1 27 vertically, exciting the FAM fluorescent group (5-Carboxyfluorescein , which is a commonly used fluorescent labeling reagent), the FAM fluorescent group emits green fluorescence when excited by blue light; then the light enters the filter 29; finally enters the transparent PMMA protective shell 22 and the magnifying glass 23. The transparent PMMA protective shell 22 plays the role of protecting the filter 29, and the magnifying glass 23 plays the role of magnifying the observation area. Both of them have no effect on the wavelength (type) of the light; the relative positions of the light path structure are: the LED light-emitting substrate 43 is at the bottom of the light path structure, the light-dispersing plate 40 is located about 12 mm above the LED light-emitting substrate 43, and the filter 1 is at the bottom of the light path structure. 27 is directly above the light homogenizing plate 40, and the two are in close contact without any gap. The reaction chamber 10 is located 4 mm directly above the filter 1 27, the filter 2 29 is located 11.5 mm directly above the reaction chamber 10, and the transparent PMMA protective shell 22 is directly above the filter 2 29, and the two are in close contact without any gap. The magnifying glass 23 is located approximately 9 mm directly above the transparent PMMA protective shell 22; the core seat body 35, the core seat bottom plate 36, the bracket 24 and the damping shaft 28 provide fixing assistance for the optical structure. When performing visual fluorescence detection, all optical structure components are parallel to each other.

[0044] In this embodiment, the LED light-emitting substrate 43 is fixed to the core base plate 36 with screws. The light diffuser 40 and filter 1 27 are vertically stacked in the mounting slot 55. Filter 2 29 is located inside the housing cover 15. A 1mm thick transparent PMMA protective shell 22 is located outside the housing cover 15 to physically protect filter 2 29. Both filter 29 and the transparent PMMA protective shell 22 are isosceles trapezoidal in shape, maximizing the viewing area.

[0045] In this embodiment, the power supply and control module includes a power supply module and a main control circuit board to realize stable power supply as well as signal acquisition and control functions. The button indication module includes two buttons, one two-color indicator light and one monochrome light to realize button control and signal display.

[0046] See also Figure 1 、 Figure 2 、 Figure 9 、 Figure 10The bottom of the main housing 16 is equipped with a bottom mounting plate 17, a battery compartment housing 18, and a battery compartment base 19. The housing cover 15 is connected to the main housing 16 via a rotating hinge 25, a fixed hinge 26, and screws. The rotating hinge 25 is screwed to the housing cover 15, and the fixed hinge 26 is screwed to the housing cover 15, ensuring that the housing cover 15 can be opened and closed. The main housing 16 and the bottom mounting plate 17 are connected by screws to ensure the housing assembly is secure. The battery compartment housing 18 and the battery compartment base 19 are also screwed to ensure the battery compartment is secure. The main housing 16 has a button indicator circuit board 38, a power switch 20, and a Type-C power port 21. The Type-C power port 21 is used with a power adapter with a power rating of 10W (5V / 2A). It can also be powered by an 18650 lithium battery (3.7V / 3000mAh), enabling dual power switching and lithium battery charging, providing stable power output and ensuring normal and stable operation. The lithium battery module 52 is housed within the battery compartment housing 18 and the battery compartment base plate 19. The bottom mounting plate 17 and battery compartment housing 18 are secured by a base plate magnet 61 located on the outside of the bottom mounting plate 17 and a battery compartment magnet 66 located on the outside of the battery compartment housing 18. This allows for secure attachment when in use, while allowing for easy removal when not in use. The overall dimensions of the device are 72*65*95mm (the removable battery compartment measures 72*65*27mm). The housing cover 15 is equipped with a cylindrical closing magnet 2 51, and the main housing 16 is equipped with a cylindrical closing magnet 1 49. When the housing cover 15 is closed, magnetic attraction ensures a stable closure. The transparent PMMA protective shell 22 is located on the surface of the shell cover 15 and is used to protect the second filter 29. The magnifying glass 23 is fixed by a bracket 24, and the bracket 24 is fixed to the shell cover 15 by a damping shaft 28. The bracket 24 can rotate around the damping shaft 28, so that you can choose whether to use the magnifying glass 23 during observation (selection basis: when using the magnifying glass 23 for observation, the observation area will be magnified to optimize the observation effect. However, since the magnifying glass 23 needs to adjust the distance between the naked eye and the magnifying glass 23 during observation to achieve focal length matching, you can also choose not to use the magnifying glass 23 and observe directly with the naked eye). When using the magnifying glass 23 for observation, rotate the bracket 24 to directly above the second filter 29. At this time, the observation area will be magnified to optimize the visual observation effect. The size of the damping shaft 28 is 4mm in diameter, 19mm in height, and the torque is 0.1N•m. It can be fixed at any angle with one hand. The magnifying glass 23 adopts an 18x magnifying glass 23 with the following dimensions: diameter 25mm, thickness 4mm. Please refer to Figure 3The microfluidic chip magnets 67 (2mm diameter, 4mm height) are symmetrically distributed on both sides of the microfluidic chip assembly 68. The purpose of designing two microfluidic chip magnets 67 is to attract the heating position magnet 48 (3mm diameter, 3mm height) and the detection position magnet 45 (3mm diameter, 3mm height). At the same time, the symmetrical design is conducive to maintaining balance when the microfluidic chip assembly 68 rotates at high speed and ensuring torque balance during magnet attraction. Figure 4 、 Figure 5 、 Figure 6, the microfluidic chip cover plate 11 and the microfluidic chip bottom plate 13 are transparent PMMA with a thickness of 1mm, and the microfluidic chip middle layer 12 is a black single-sided frosted PMMA with a thickness of 2mm. Compared with the mirror material PMMA, the frosted material can effectively reduce the scattering of incident light and reduce interference. The mirror surface of the microfluidic chip middle layer 12 is combined with the microfluidic chip cover plate 11, and the frosted surface of the microfluidic chip middle layer 12 is combined with the microfluidic chip bottom plate 13. Specifically, when the test agent is injected, it enters from the sample injection hole 1 and passes through the second channel 5 of each detection unit in turn, filling the quantitative cavity 8 of each detection unit. The depth of the first channel 9 of the microfluidic chip middle layer 12 and the buffer cavity 7 is 0.4mm, and the width of the first channel 9 is 0.24mm. The radius of the buffer cavity 7 is 0.3mm, which prevents the magnetic beads and dry powder pre-placed in the reaction chamber 10 from entering other chambers. Compared to the second channel 5 and quantification chamber 8, the first channel 9 and buffer chamber 7 are tiny channels (the second channel 5 is 0.45 mm wide and 2 mm deep; the quantification chamber 8 is 2 mm deep; the volume of the second channel 5 and quantification chamber 8 for each detection unit is approximately 25 μl). These channels offer greater resistance to fluid flow, ensuring that reagents or samples do not break through and enter the reaction chamber 10 during injection. The quantification chamber 8 and second channel 5 are filled first, achieving quantification of reagents for each detection unit. Furthermore, the tiny size of the first channel 9 and buffer chamber 7 prevents loss of the magnetic beads and dry powder placed in the reaction chamber 10 (the reaction chamber 10 is 2 mm deep and has a volume of approximately 25.8 μl; the volume of the magnetic beads is approximately 0.5 μl; the volume of the dry powder pellets is approximately 0.3 μl). After reagent injection is completed, the sample injection port 1 and air hole 3 are sealed. The microfluidic chip assembly 68 is placed in the tight-fitting shaft sleeve 44 above the centrifugal motor 41. The centrifugal motor 41 begins centrifugation, and the microfluidic chip assembly 68 performs high-speed circular motion around the motor shaft. At this point, the reagents in the quantitative chamber 8 and second channel 5 of each detection unit undergo gas-liquid exchange through the first channel 9 and buffer chamber 7, entering the reaction chamber 10. At this point, the reaction chamber 10 is completely filled with reagents, including magnetic beads, and the dry powder dissolves. After the reagents enter the reaction chamber 10, the quantitative chamber 8 and second channel 5 of the microfluidic chip become empty, providing air isolation between the reaction chambers 10 during heat amplification. The centrifugal motor 41 is a small DC coreless cup motor with a diameter of 15 mm and a height of 17 mm (including the motor shaft). It is supplied with a 3.3V voltage. The supply voltage to the centrifugal motor 41 can be controlled by outputting a PWM duty cycle signal from the main control board 39, thereby adjusting the motor speed. When the device is operating in centrifugal mode, the PWM duty cycle is adjusted to 100%. At this point, the voltage across centrifugal motor 41 is 3.3V, and the motor 41 is rotating at its maximum speed, thus achieving high-speed centrifugation. Reagent injection, quantitative reagent distribution across the four detection channels, and centrifugation require only a single injection and sealing of pore 3; subsequent operations are automated.The fixing hole 16 and the shaft sleeve 44 in the microfluidic chip assembly 68 are both circular segments. The angles of the fixing hole 16 and the shaft sleeve 44 match, ensuring that the microfluidic chip assembly 68 does not move relative to each other during high-speed rotation. The microfluidic chip base plate 13 is a simple load-bearing structure, featuring a thermal insulation groove 4, a positioning hole 14, a bonding positioning hole 2, and a fixing hole 16. The PCB heater circuit board 34, centrifugal motor 41, and filter 1 27 are secured by support columns 57 on the surface of the core base body 35. The front surface of the PCB heater circuit board 34 (with the component layer) faces the surface of the core base body 35, while the back surface of the PCB heater circuit board 34 (the flat surface with the window opening) is used for heating and faces the microfluidic chip assembly 68. The support columns 57 elevate the PCB heater circuit board 34, eliminating contact with the surface of the core base body 35, reducing heat transfer and improving heating efficiency. The wiring harness for the PCB heater circuit board 34 is connected to the main control board 39 through the device wiring harness hole 3 56. After reagent injection, quantitative reagent dispensing, and centrifugation, the microfluidic chip assembly 68 is controlled to the heating position through the interaction of the centrifuge motor 41, the Hall sensor 33, the heating position magnets 48, and the microfluidic chip magnets 67. When any one of the microfluidic chip magnets 67 is attracted to the corresponding heating position magnet 48, the microfluidic chip assembly 68 is in the heating position. Directly below the reaction chambers 10 of its four detection units are the four heating zones of the PCB heating circuit board 34. The specific execution process of the centrifugal motor 41 is as follows: the main control board 39 outputs a PWM duty cycle of 10% at this time, reducing the speed of the centrifugal motor 41. At this time, the microfluidic chip assembly 68 starts to rotate at a low speed. When the microfluidic chip magnet 67 rotates through the Hall sensor 33, the main control board 39 will detect the signal and then adjust the PWM duty cycle to 0%. At this time, the centrifugal motor 41 stops rotating. At the same time, due to the certain inertia of the microfluidic chip assembly 68 and the heating position magnet 48 fixed to the main shell 16, this will attract the microfluidic chip magnet 67, and finally realize the rotation of the microfluidic chip assembly 68 to a fixed angle, so that it stops at the heating position. At this time, the reaction chamber 10 of each detection unit in the microfluidic chip assembly 68 is located directly above the rectangular heating area of ​​the PCB heating circuit board 34, and non-contact heating amplification can be started at this time. Please refer to. Figure 9 、 Figure 10 、 Figure 13, after the microfluidic chip assembly 68 is placed, the relative positions of the core seat body 35, the hybrid drive component 32, the magnetic fixing frame 30 and the Hall sensor 33. The microfluidic chip assembly 68 is located above the PCB heating circuit board 34, with a spacing of 0.8mm. Non-contact heating is adopted so that the heating and rotation of the microfluidic chip assembly 68 do not interfere with each other, simplifying the structural design. (Non-contact heating mainly transfers energy to the target object through electromagnetic radiation, transfers it to the surface of the object and converts it into heat, causing it to heat up). The hybrid drive component 32 is fixed to the shell cover 15 by screws, and a fixing frame 30 is fixed on the hybrid drive component 32. Mixing magnets 31 (with a diameter of 3mm and a height of 3mm) are fixed at both ends of the fixing frame 30. The purpose of fixing the mixing magnets 31 at both ends is to improve the mixing efficiency of the magnetic beads and to keep the fixing frame 30 balanced during rotation. After closing the housing cover 15, the mixing drive 32 is positioned directly above the microfluidic chip assembly 68. Rotating the mixing drive 32 drives the fixed element and the mixing magnets 31 at both ends to perform circular motion around the motor shaft. The motion trajectory of the mixing magnets 31 covers the reaction chambers 10 of each detection unit in the microfluidic chip assembly 68, thereby mixing the magnetic beads pre-embedded in the reaction chambers 10 and improving the efficiency of the amplification reaction. During heat amplification, the magnetic bead mixing process can be set to occur every two minutes. The mixing drive 32 is a small DC coreless motor with a diameter of 6 mm and a height of 16.5 mm (including the motor shaft). The supply voltage is controlled by controlling the PWM duty cycle signal output by the main control board 3939, thereby adjusting the motor speed. Because the motor's primary function is to mix magnetic beads, its speed is not too high. Testing has shown that when the PWM duty cycle is adjusted to 20%, the mixing magnets 31 achieve optimal adsorption of magnetic beads in the microfluidic chip assembly 68. Therefore, the PWM duty cycle output by the main control board 39 is set to 20%, and the speed of the mixing drive component 32 is relatively appropriate at this time. At the same time, there is non-contact between the mixing magnet 31 and the microfluidic chip assembly 68. While realizing the function of mixing the magnetic beads in the microfluidic chip assembly 68, it does not affect the circular motion of the microfluidic chip assembly 68, and also reduces the heat loss of the microfluidic chip assembly 68 to the outside during amplification. The spacing between the mixing magnet 31 and the microfluidic chip assembly 68 is 0.5mm. The centrifugal motor 41 and the LED light-emitting substrate 43 are located between the core seat body 35 and the core seat bottom plate 36, wherein the centrifugal motor 41 is fixed to the core seat body 35 with screws through the fixing hole 458, and the LED light-emitting substrate 43 is fixed to the core seat bottom plate 36 with screws. The rotating parts of the centrifugal motor 41 and the shaft sleeve 44 extend out of the surface of the core seat body 35 through the shaft hole 59. The core seat body 35 and the core seat bottom plate 36 realize multi-part fixation, and the non-contact heating component, the centrifugal motor 41 and the excitation light part of the fluorescence detection component are fixedly placed in a small space through appropriate structural design.The wiring harness for the PCB heating circuit board 34, centrifugal motor 41, and LED light-emitting substrate 43 is connected to the main control board 39 through wiring harness hole 1 42, completing the wiring harness management and facilitating modular installation. The wiring harness for the PCB heating circuit board 34 is connected to the main control board 39 through wiring harness hole 3 56. See. Figure 14 、 Figure 15 Filter 2 29 is located on the inner side of the shell cover 15, and the transparent PMMA protective shell 22 with a thickness of 1mm is located on the outer side of the shell cover 15, which plays the role of protecting filter 2 29. Filter 2 29 uses a long-wave pass filter with a cut-off wavelength of 506nm and a cut-off depth of OD3. Its main function is to retain the excitation light of the FAM fluorescent group and filter out stray light in the blue band, which is conducive to the direct observation of the MIRA amplification results using the FAM fluorescent group. Two filters are superimposed to optimize the cut-off effect. The size of a single filter is: an isosceles trapezoid with an upper base of 7mm, a lower base of 28mm, a height of 23mm, and a thickness of 1.1mm. The shapes of filter 2 29 and the transparent PMMA protective shell 22 are both isosceles trapezoids, which maximize the observation area and are conducive to visual observation. Please refer to Figure 16 、 Figure 17, fixing hole two 50 is responsible for fixing the damping shaft 28, and a magnifying glass 23 can be provided to observe the MIRA amplification results. Mounting hole one 47 is responsible for fixing the Hall sensor 33; mounting hole two 53 is responsible for fixing the hybrid drive component 32 on the shell cover 15 and managing the wiring harness. The wiring harness of the hybrid drive component 32 is connected to the main control board 39 through the wiring harness hole two 46. Any one of the microfluidic chip magnets 67 is adsorbed correspondingly to the detection position magnet 45. At this time, the microfluidic chip assembly 68 is in the detection position, and the reaction chamber 10 of one of the four detection units is directly below the filter one 27. The device has a total of four detection position magnets 45, which are symmetrically distributed and the angle between them is 90 degrees. When the microfluidic chip assembly 68 is in the detection position, it needs to be rotated 90 degrees to allow the reaction chamber 10 of the next detection unit to enter the detection position, and the above steps are repeated until all the detection unit reaction chambers 10 are detected. When fluorescence detection begins, the LED light-emitting substrate 43 is turned on, and the speed of the centrifugal motor 41 is adjusted at the same time. The microfluidic chip magnet 67 and the detection position magnet 45 can be used to rotate the microfluidic chip assembly 68 to a fixed angle, so each reaction chamber 10 can be rotated to directly above the filter 1 27. At this time, the LED light-emitting substrate 43, the light-homogenizing plate 40, the filter 1 27, the reaction chamber 101, the filter 2 29, and the magnifying glass 23 form a vertical optical path structure, and visual observation can begin. The microfluidic chip assembly 68 has a total of four groups of detection units, and the angle between two adjacent reaction chambers 10 is 90 degrees. Therefore, the centrifugal motor 41 controls the microfluidic chip assembly 68 to rotate a fixed angle to complete the detection of all detection units. The specific operation of the centrifugal motor 41 is as follows: Starting from the heating position, the microfluidic chip assembly 68 starts with the main control board 39 outputting a 74% PWM duty cycle for 30ms, providing sufficient torque to disengage the heating position magnet 48. The PWM duty cycle is then reduced to 4% for 100ms, causing the centrifugal motor 41 to rotate slowly, performing a deceleration process. The PWM duty cycle is then reduced to 0%, allowing inertial attraction to the detection position magnet 45. This is a rotation detection process. The microfluidic chip assembly 68 has four detection units, which can be rotated according to specific needs or automatically rotated. For convenient observation, each detection unit rotates at a 10s interval. The four symmetrical holes in the fixing holes 54 mate with the core base plate 36 and are secured with screws. The Hall effect sensor 33 is mounted inside the main housing 16 and embedded in the mounting hole 1 47. The main control board 39 is secured to the bottom mounting plate 17 with screws, while the power switch 20 and the key indicator circuit board 38 are also secured to the main housing 16 with screws.The power module and the dual power switching module are responsible for the overall power supply of the device. The device can be powered by a Type-c power adapter (5V / 2A) or an 18650 lithium battery (3.7V / 3000mAh). The device can realize dual power switching between the power adapter and the lithium battery and battery charging. The power adapter and the lithium battery can both be powered separately. When both are connected at the same time, the lithium battery can be charged. The Bluetooth communication module is responsible for data communication with the host computer software; the button indication circuit board 38 is responsible for the human-computer interaction of the device hardware, realizing button detection and related indication LED display. There are two LED indicators, one is red There is a single-color LED indicator 63, and a red and blue dual-color LED indicator 62. The single-color light serves as the device's power indicator, the red color of the dual-color light serves as the device's heating and amplification indicator, and the blue color serves as the fluorescence detection process indicator. There are two button caps 64, namely the heating and amplification button 37 and the fluorescence detection button 65, one for starting and stopping the heating and amplification, and the other for starting and stopping the fluorescence detection process. The position detection module is responsible for acquiring the signal detected by the Hall sensor 33. The buzzer alarm module is responsible for sounding an alarm to alert the device user after the heating and amplification is completed. The power switch 20 is responsible for turning the device's main power on and off. The wiring harness hole 4 60 is responsible for the wiring harness connection between the main control board 39 and the lithium battery module 52. The bottom mounting plate 17 is equipped with a bottom plate magnet 61 on the outside. The relative positions of the battery compartment shell 18, the battery compartment bottom plate 19, and the lithium battery module 52 are shown. The lithium battery module 52 is placed inside the battery compartment shell 18 and the battery compartment bottom plate 19. A battery compartment magnet 66 is mounted on the outside of the battery compartment housing 18, which attracts the bottom plate magnet 61 of the bottom mounting plate 17. When using a lithium battery, it is securely attached and can be removed without any complicated operations when not in use. The PCB heating circuit board 34 integrates a temperature measurement chip 70 and a heating resistor 69, providing four heating zones. The supply voltage is 5V, the total heating power is 6.1W, and the resistance of each heating resistor 69 is 33 ohms, the component package is 2010, and the rated power is 1.5W. The PCB heating circuit board 34 has four heating zones, located in four rectangular areas on the back of the PCB heating circuit board 34. Each heating zone has a 90-degree angle and measures approximately 10 x 9 mm, corresponding to the reaction chambers 10 of the four detection units of the microfluidic chip assembly 68. When installed, the PCB heating circuit board 34 faces downward (the side with the components) and upward (the side facing the microfluidic chip base plate 13), achieving non-contact heating. The microfluidic chip assembly 68 is located above the PCB heating circuit board 34, with a spacing of 0.8 mm.On the front of the PCB heating circuit board 34, each heating zone consists of two heating resistors 69 and a temperature measuring chip 70. The heating resistors 69 are symmetrically and evenly distributed throughout the heating zone, with the temperature measuring chip 70 positioned between them. This symmetrical layout ensures uniformity across the heating zones, while the temperature measuring chip 70 is positioned at the center of each zone, ensuring accurate temperature measurement. Each heating zone in the PCB heating circuit is larger than the microfluidic chip's reaction chamber 10 (each measuring approximately 5.1 x 2.8 mm), effectively covering the entire area of ​​the reaction chamber 10 and ensuring efficient and stable heating amplification. The remaining dimensions of the PCB heating circuit board 34 (including the connections between the different heating zones, the terminal blocks 71, and the fixing holes 72) are minimized to reduce heat loss and improve heating efficiency within each zone. The PCB heating circuit board 34 has a ring structure, but is designed with a notch (at an angle of approximately 47 degrees) to leave an angle for the visual fluorescence detection light path. There will be a visual fluorescence detection optical module in the vertical direction of the notch. This notch can cooperate with the different reaction chambers 10 in the microfluidic chip assembly 68 to rotate to the fluorescence detection position to start visual fluorescence detection.

[0047] Example 2. Based on Example 1, this example provides a detection method using a multi-target detection device suitable for a microfluidic chip as provided in Example 1, which includes: driving the microfluidic chip component 68 to rotate through a centrifugal component to centrifuge the test agent in the microfluidic chip component 68 to a corresponding position; controlling the temperature in the microfluidic chip component 68 through a heating component to amplify the test agent in the microfluidic chip component 68; using a reaction mixing component to intermittently attract magnetic beads in the microfluidic chip component 68 through magnetic force to move, so that the test agent in the microfluidic chip component 68 is mixed with a fluorescent labeled reagent; emitting detection light to the microfluidic chip component 68 through a fluorescent detection component, and outputting corresponding detection results through an observation component.

[0048] In summary, the present invention can quickly centrifuge and heat-amplify the test agent through the non-contact setting of the heating component and the microfluidic chip component, and the reaction mixing component can evenly mix the test agent and the fluorescent labeling reagent. At the same time, it cooperates with the fluorescence detection component and the observation component to realize rapid multi-target detection and automation of the detection process. It has a small size, low cost, simple structure, and is easy to operate and carry. It uses a visual fluorescence detection method to complete the detection of multiple detection units in the centrifugal microfluidic chip and complete multi-target detection. At the same time, through the optomechanical integration of the instrument device, the device operation is automated to be suitable for POCT.

[0049] The various devices selected in this application (components whose specific structures are not described) are all universal standard parts or components known to those skilled in the art, and their structures and principles can be known to those skilled in the art through technical manuals or conventional experimental methods.

[0050] In the description of the embodiments of the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed, detachable, or integral connections; mechanical or electrical connections; direct or indirect connections through an intermediate medium; and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0051] In the description of the present invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate and simplify the description of the present invention. They are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation, and therefore should not be construed as limitations on the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0052] The units described as separate components may or may not be physically separate, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed across multiple network units. Some or all of these units may be selected to achieve the purpose of this embodiment according to actual needs.

[0053] With the above-described preferred embodiments of the present invention as a guide, and with reference to the above description, relevant personnel are fully capable of making various changes and modifications without departing from the technical scope of this invention. The technical scope of this invention is not limited to the contents of the specification and must be determined according to the scope of the claims.

Claims

1. A multi-target detection device suitable for a microfluidic chip, characterized in that: include: Housing assembly, heating assembly, centrifugal assembly, fluorescence detection assembly, microfluidic chip assembly, reaction mixing assembly and observation assembly; in The heating component, centrifugal component, fluorescence detection component, microfluidic chip component, and reaction mixing component are installed in the housing component, the heating component, centrifugal component, and fluorescence detection component are located below the microfluidic chip component, the centrifugal component is connected to the microfluidic chip component, and the reaction mixing component is located above the microfluidic chip component; The observation assembly is installed on the outside of the housing assembly, and the observation assembly is located on the optical path of the fluorescence detection assembly; The centrifugal assembly drives the microfluidic chip assembly to rotate, so as to centrifuge the test agent in the microfluidic chip assembly to a corresponding position; The heating component controls the temperature in the microfluidic chip component so as to amplify the test agent in the microfluidic chip component; The reaction mixing component intermittently attracts the magnetic beads in the microfluidic chip component through magnetic force to move, so that the test agent in the microfluidic chip component is mixed with the fluorescent labeling reagent; as well as The fluorescence detection component emits detection light to the microfluidic chip component, and outputs corresponding detection results through the observation component; The heating assembly includes: a PCB heating circuit board; The PCB heating circuit board is an annular structure, and a notch is provided on the PCB heating circuit board, and a fluorescence detection component is provided in a vertical direction corresponding to the notch; The centrifugal assembly includes: a centrifugal motor, a Hall sensor, a main control board, a heating position magnet, a plurality of detection position magnets and two microfluidic chip magnets; The two microfluidic chip magnets are symmetrically distributed along the diameter of the microfluidic chip assembly; The reaction mixing assembly includes: a mixing drive, a fixing frame, a plurality of mixing magnets and a plurality of magnetic beads; The hybrid drive member is located in the second groove on the housing cover of the housing assembly, and the hybrid drive member is connected to the housing cover; The fixing frame is connected to the hybrid drive element, and each of the hybrid magnets is mounted on the fixing frame; Each of the magnetic beads is located in a corresponding reaction chamber in the microfluidic chip assembly; The main control board is configured to sense the microfluidic chip magnet through a Hall sensor, so that after sensing the microfluidic chip magnet, the main control board is further configured to output a PWM duty cycle signal to adjust the speed of the centrifugal motor, so that the microfluidic chip assembly rotates slowly until any of the microfluidic chip magnets is attracted to the heating position magnet, that is, the microfluidic chip assembly stops at the heating position; When the microfluidic chip assembly is in the heating position, the reaction chambers of the detection units in the microfluidic chip assembly are respectively located directly above the corresponding heating areas on the PCB heating circuit board; The main control board is further configured to output a PWM duty cycle signal to adjust the rotation speed of the centrifugal motor, so that the microfluidic chip assembly rotates slowly until any of the microfluidic chip magnets is attracted to the detection position magnet, that is, the microfluidic chip assembly stops at the detection position; When the microfluidic chip assembly is located at the detection position, the reaction chamber of a detection unit in the microfluidic chip assembly is located at the notch on the PCB heating circuit board, so that the fluorescence detection assembly performs visual fluorescence detection at the notch.

2. The multi-target detection device suitable for a microfluidic chip according to claim 1, characterized in that: The housing assembly comprises: a main housing and a housing cover; The shell cover is hinged to the top of the main shell; A slot 1 is formed on the top of the main shell for loading the heating component, centrifugal component, fluorescence detection component, and microfluidic chip component; The shell cover is provided with a second groove for receiving the reaction mixing assembly; The observation component is movably mounted on the shell cover, and a window 1 communicating with the groove 2 is provided on the shell cover.

3. The multi-target detection device suitable for a microfluidic chip according to claim 2, characterized in that: The microfluidic chip assembly includes: a microfluidic chip cover plate, a microfluidic chip middle layer, and a microfluidic chip bottom plate; The microfluidic chip cover plate, the microfluidic chip middle layer, and the microfluidic chip bottom plate are stacked in sequence from top to bottom; The microfluidic chip cover is provided with a sample addition hole, and the middle layer of the microfluidic chip is provided with a detection hole; The microfluidic chip cover plate and the microfluidic chip bottom plate are solidly arranged at positions corresponding to the detection holes, and the sample addition holes are communicated with the detection holes.

4. The multi-target detection device suitable for a microfluidic chip according to claim 3, characterized in that: The microfluidic chip cover is provided with air holes, which are communicated with the detection holes.

5. The multi-target detection device suitable for a microfluidic chip according to claim 3, characterized in that: The detection hole is divided into several sub-holes; The sub-wells include a quantitative well, a first connecting well, a buffer well, a second connecting well and a reaction well; The quantitative hole, the first connecting hole, the buffer hole, the second connecting hole, and the reaction hole respectively form a quantitative cavity, a first channel, a buffer cavity, a second channel, and a reaction cavity with the microfluidic chip cover plate and the microfluidic chip bottom plate; Any of the quantitative chambers is connected to the adjacent quantitative chambers through the second channel, and the quantitative chambers are connected to the reaction chamber through the first channel and the buffer chamber.

6. The multi-target detection device suitable for a microfluidic chip according to claim 2, wherein: The fluorescence detection assembly includes: an LED light output substrate, a light homogenizing plate, a first filter, and a second filter; The LED light output substrate, the light homogenizing plate, and the first filter are sequentially arranged below the microfluidic chip assembly from bottom to top, the second filter is installed in the first window, and the microfluidic chip assembly is located between the first filter and the second filter.

7. The multi-target detection device suitable for a microfluidic chip according to claim 6, characterized in that: The observation assembly includes: a magnifying glass; The magnifying glass is mounted on the housing assembly, and the viewing direction of the magnifying glass is arranged toward the second filter.

8. The multi-target detection device suitable for a microfluidic chip according to claim 7, characterized in that: The magnifying glass is movably connected to the housing assembly through a bracket and a damping shaft.

9. A detection method using the multi-target detection device suitable for a microfluidic chip according to any one of claims 1 to 8, characterized in that: include: The microfluidic chip assembly is driven to rotate by the centrifugal assembly to centrifuge the test agent in the microfluidic chip assembly to the corresponding position; Controlling the temperature in the microfluidic chip assembly by a heating assembly so as to amplify the test agent in the microfluidic chip assembly; The reaction mixing component intermittently attracts the magnetic beads in the microfluidic chip component by magnetic force to move, so that the test agent in the microfluidic chip component is mixed with the fluorescent labeling reagent; The fluorescence detection component sends detection light to the microfluidic chip component, and the observation component outputs the corresponding detection result.

Images