Compound reed root lung-clearing composition, preparation and preparation method thereof

Abstract

The present invention discloses a compound reed root lung-clearing composition, a preparation, and a preparation method thereof. The compound reed root lung-clearing composition comprises, by weight, 20-45 parts reed root, 5-11 parts momordica grosvenori, 7-26 parts bamboo sap, 4-20 parts stir-fried bitter almonds, 4-20 parts cornus officinalis, 6-20 parts platycodon grandiflorum, 10-22 parts mulberry, 18-36 parts tremella, 5-18 parts polygonatum, and 5-18 parts wolfberry. The compound reed root lung-clearing composition and preparation are effective for treating chronic obstructive pulmonary disease, particularly for lung and kidney yin deficiency in the stable phase of chronic obstructive pulmonary disease.

Description

Technical Field

[0001] The present invention belongs to the technical field of drugs for treating chronic obstructive pulmonary disease, and particularly relates to a compound reed root lung-clearing composition, a preparation and a preparation method thereof for treating lung and kidney yin deficiency syndrome of chronic obstructive pulmonary disease. Background Art

[0002] Chronic obstructive pulmonary disease (COPD) is a chronic, progressive, destructive disease of the airways and lung tissue. It primarily includes chronic bronchitis and emphysema. Smoking is one of the main pathogenic factors of COPD. It has high morbidity, disability, and mortality rates, a heavy socioeconomic burden, and a serious threat to public health. The prevalence of COPD is 10% in people over 45 years old, rising to 50% in heavy smokers. Approximately 3 million people die from COPD each year worldwide, accounting for 6% of the total annual death toll. Its causes include smoking, air pollution, childhood cough, and genetics, while its mechanisms are related to inflammation, oxidative stress, and immune disorders.

[0003] COPD corresponds to the categories of "asthma" and "lung distension" in Traditional Chinese Medicine. The lungs govern qi, opening to the nose and connecting to the skin, and are responsible for protecting the exterior. Therefore, when external pathogens invade through the mouth, nose, and skin, they often first attack the lungs, causing lung qi to stagnate and rise, resulting in coughing. Impaired ascending and descending qi leads to asthma. Prolonged retention of pathogenic heat leads to lung deficiency and damage to the kidneys, the root of qi intake. Over time, this damage extends to the kidneys, resulting in lung and kidney yin deficiency. Furthermore, damage to yin leads to loss of lung qi, and insufficient lung qi affects the spleen. This theft of the mother's qi by the child causes the spleen to lose its healthy function, leading to lung and spleen qi deficiency. From the perspective of Traditional Chinese Medicine etiology and pathogenesis, the progression from phlegm-heat stagnation in the lungs to yin damage to lung and kidney yin deficiency, and finally to lung and spleen qi deficiency, is a process of progressive worsening, from yin damage to qi and yang damage. Summary of the Invention

[0004] The applicant, through extensive literature research and clinical investigations, previously discovered that lung and kidney yin deficiency is a common symptom of COPD in the stable phase. Based on this finding and in conjunction with long-term clinical practice, they developed a compound reed root lung-clearing composition for treating COPD, characterized by lung failure to clear and purge, and kidney failure to absorb. Clinically, this composition has been shown to effectively improve COPD patients' clinical symptoms, reduce the frequency of acute exacerbations, and enhance immunity. The present invention also provides a preparation using this compound reed root lung-clearing composition as a raw material and a scientifically feasible preparation method for the preparation. This approach further strengthens the research and development of traditional Chinese medicine preparations for lung and kidney yin deficiency in the stable phase of COPD, and is of great significance for improving clinical prevention and treatment capabilities.

[0005] In view of the above content, in order to solve the defects of the prior art, the purpose of the present invention is to provide a compound reed root lung-clearing composition, a preparation using the composition as raw material and a preparation method thereof. The compound reed root lung-clearing composition and preparation can effectively treat lung and kidney yin deficiency syndrome in the stable stage of COPD, and can effectively solve the problem of medication for the treatment of chronic obstructive pulmonary disease.

[0006] The present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a compound reed root lung-clearing composition, which is one of the following compound reed root lung-clearing compositions I-IV.

[0008] The compound rhizome of reed to clear lung composition I comprises, in parts by weight:

[0009] 20-45 parts of reed rhizome, 5-11 parts of momordica grosvenori, 7-26 parts of bamboo juice, 4-20 parts of stir-fried bitter almonds, 4-20 parts of cornus officinalis, 6-20 parts of platycodon grandiflorum, 10-22 parts of mulberry, 18-36 parts of white fungus, 5-18 parts of polygonatum, and 5-18 parts of wolfberry.

[0010] In some embodiments, the compound rhizome of reed to clear lung composition I comprises, in parts by weight:

[0011] 45 parts of reed rhizomes, 11 parts of momordica grosvenori, 26 parts of bamboo sap, 20 parts of stir-fried bitter almonds, 20 parts of cornus officinalis, 20 parts of platycodon grandiflorum, 22 parts of mulberries, 36 parts of white fungus, 18 parts of polygonatum, and 18 parts of wolfberry fruits.

[0012] In some embodiments, the compound reed rhizome lung-clearing composition I is composed of the above components.

[0013] In the formula of the compound reed rhizome lung-clearing composition I of the present invention, reed rhizome, momordica grosvenori and bamboo sap are monarch drugs, mulberry, cornus officinalis, polygonatum and wolfberry are ministerial drugs, tremella fuciformis is adjuvant drug, and platycodon grandiflorum and fried bitter almond are guiding drugs.

[0014] In the formula, reed root is sweet and cold in nature, entering the lung and stomach meridians. It moistens the lungs and relieves coughs, clears heat and promotes fluid production, relieves restlessness, and stops vomiting. It primarily treats heat in the lung and stomach meridians, qi reversal, vomiting, and coughing with sticky phlegm, as well as phlegm-heat congestion in the lungs. Huang Yuanyu's "Yuzhou Yaojie" states: "This medicine can reduce adverse qi and stop vomiting, clear heat and relieve restlessness, clear the lungs and stomach, dissipate depression and restlessness, promote fluid production and quench thirst, relieve vomiting and promote indigestion, and treat choking, hiccups, and irritability." Momordica grosvenori (Luo Han Guo) is sweet and cool in nature, entering the lung and large intestine meridians. It clears heat and benefits the lungs, relieves sore throat and improves voice, and moistens the intestines and promotes bowel movements. Its lung-clearing and intestinal-moistening properties are particularly effective in treating whooping cough, cough due to phlegm-heat, and constipation caused by dry blood. The combination of reed root and Momordica grosvenori clears heat, moistens the lungs, relieves coughs, and promotes bowel movements, allowing lung qi to descend and cough and asthma to subside. Bamboo juice is sweet and cold in nature, entering the heart, lung, and liver meridians to clear the lungs and resolve phlegm, calm nerves and improve the senses. Huang Yuanyu's "Yuzhou Yaojie" states: "Bamboo juice is sweet, cold, and dispersing, clearing the chest and diaphragm, relieving thirst, and dissolving sticky phlegm. It treats heart and lung heat stagnation and obstructed orifices caused by stroke. It is also effective in treating upper-burner deficiency fire that dries up phlegm, and helps reed root dissolve sticky phlegm in the lungs." These three herbs are collectively referred to as the "main ingredient" in the formula.

[0015] The lungs are the master of qi, and the kidneys are the root of qi. The lungs control exhalation, while the kidneys control inhalation. The harmony of yin and yang in exhalation and inhalation results in balanced breathing. The descending of lung qi aids the kidney's inhalation of qi. Sufficient kidney essence and robust inhalation allow lung qi to descend. This mutual reliance ensures normal respiratory function. The formula incorporates mulberry, cornus officinalis, polygonatum, and wolfberry as auxiliary herbs, which can aid the lung's descending of qi, inhalation of qi, and calm asthma. This allows lung qi to descend, providing a rooted inhalation, eliminating phlegm and alleviating cough. Mulberry, with its sweet and sour flavor and cold nature, enters the liver and kidney meridians, nourishing the liver and kidney's yin and blood, promoting fluid production and moistening dryness. Huang Yuanyu's "Yu Qiu Yao Jie" states: "Mulberry nourishes wood, promotes water excretion, and clears wind and moistens dryness." Cornus officinalis, with its sour and astringent flavor and slightly warm nature, enters the liver and kidney meridians, nourishing yin and supporting yang, nourishing blood and astringing essence. It enters the liver and gallbladder, nourishing blood, and is a nourishing herb, essential for tonifying the liver and warming the kidneys." Ye Tianshi's "Compendium of Materia Medica" records that "Cornus officinalis has a neutral energy, endowed with the metal energy of autumn, entering the hand Taiyin Lung Meridian. It has a sour and non-toxic flavor, and possesses the woody flavor of the east, entering the foot Jueyin Liver Meridian. Its energy descends, reflecting its yin nature. Therefore, it nourishes the liver and helps the lungs descend." The two herbs, taken together, replenish kidney essence and blood, yin and yang intertwined. Cornus officinalis can also nourish the kidney and support yang, achieving its function of absorbing qi and calming asthma. Polygonatum sibiricum combined with mulberry can replenish both acquired and acquired essence. Because Polygonatum sibiricum is sweet and neutral in nature, it enters the spleen, lung, and kidney meridians, strengthening the spleen, moistening the lungs, and benefiting the kidneys. Its combination with mulberry can both replenish acquired essence and strengthen it. Li Zhongzi's "Leigong Paoyi Yaoxing Jie" records that Polygonatum sibiricum nourishes the middle and replenishes qi, eliminates rheumatism, soothes the five internal organs, maintains complexion, and prolongs life. Its sweetness favors the spleen, while its moistness favors the lungs. Long-term use is crucial for achieving its benefits. Lycium barbarum enters the liver and kidney meridians, nourishing them. Its red color and bitter taste benefit the heart, making it a yang medicine. When taken with Cornus officinalis, it can nourish kidney essence and strengthen kidney yang. Ye Tianshi's "Commentary on the Classic of Materia Medica" states: "Long-term consumption of bitterness benefits the heart, while coldness benefits the kidneys. When the heart and kidneys interact, water and fire are calmed, and tendons and bones are strengthened."

[0016] Adding Tremella fuciformis, a medicinal and edible herb, prevents stomach upset from cold foods. Tremella fuciformis nourishes and promotes fluid production, moistening the lungs and nourishing the stomach, treating lung-heat coughs and dry coughs. The Chinese Materia Medica states: "It is primarily used to treat consumptive coughs, and thirst due to lack of fluid." It complements the main herb, moistening the lungs and relieving coughs.

[0017] Platycodon grandiflorum and stir-fried bitter almonds serve as guiding herbs. Platycodon grandiflorum is pungent, dispersing, bitter, and laxative, with a neutral nature and a tendency to ascend. It specifically enters the lung meridian, guiding the other herbs to the site of the disease, effectively promoting lung qi and dispersing phlegm and pus. Almonds, on the other hand, are bitter, warm, and moistening, descending, entering the lung and large intestine meridians, lowering lung qi and relieving cough and asthma. Platycodon grandiflorum and almonds, one ascending and one descending, conform to the lung's physiological function of dispersing, descending, and clearing phlegm and cough, clearing toxins and heat, and ensuring controlled exhalation and rooted inhalation, thus eliminating all symptoms.

[0018] In some embodiments, based on the compound reed root lung-clearing composition I, polygonatum is removed and raw rehmannia root with similar medicinal properties is added to obtain the compound reed root lung-clearing composition II, that is, it comprises, in parts by weight:

[0019] 20-45 parts of reed rhizome, 5-11 parts of momordica grosvenori, 7-26 parts of bamboo juice, 4-20 parts of stir-fried bitter almonds, 4-20 parts of cornus officinalis, 6-20 parts of platycodon grandiflorum, 10-22 parts of mulberry, 18-36 parts of white fungus, 5-18 parts of raw rehmannia root, and 5-18 parts of wolfberry.

[0020] In some embodiments, the compound reed root lung-clearing composition II comprises, by weight: 45 parts of reed root, 11 parts of momordica grosvenori, 26 parts of bamboo juice, 20 parts of fried bitter almonds, 20 parts of cornus officinalis, 20 parts of platycodon, 22 parts of mulberry, 36 parts of tremella, 18 parts of raw rehmannia, and 18 parts of wolfberry.

[0021] In some embodiments, the compound reed rhizome lung-clearing composition II is composed of the above components.

[0022] In some embodiments, based on the compound reed root lung-clearing composition I, bamboo juice is removed and raw rehmannia root is added to obtain the compound reed root lung-clearing composition III, that is, it comprises, in parts by weight:

[0023] 20-45 parts of reed rhizome, 5-11 parts of momordica grosvenori, 4-20 parts of stir-fried bitter almonds, 4-20 parts of cornus officinalis, 6-20 parts of platycodon grandiflorum, 10-22 parts of mulberry, 18-36 parts of white fungus, 5-18 parts of polygonatum, 5-18 parts of raw rehmannia root, and 5-18 parts of wolfberry.

[0024] In some embodiments, the compound reed root lung-clearing composition III comprises, in parts by weight: 45 parts of reed root, 11 parts of momordica grosvenori, 20 parts of fried bitter almonds, 20 parts of cornus officinalis, 20 parts of platycodon grandiflorum, 22 parts of mulberry, 36 parts of tremella, 18 parts of polygonatum, 18 parts of raw rehmannia, and 18 parts of wolfberry.

[0025] In some embodiments, the compound reed root lung-clearing composition III is composed of the above components.

[0026] In some embodiments, based on the compound reed root lung-clearing composition I, 5-18 parts of raw rehmannia root and 5-18 parts of thunbergia thunbergii are added to increase the yin-nourishing effect and the efficacy of expectorant, cough and asthma, to obtain the compound reed root lung-clearing composition IV, that is, it comprises, in parts by weight:

[0027] 20-45 parts of reed rhizome, 5-11 parts of momordica grosvenori, 7-26 parts of bamboo juice, 4-20 parts of stir-fried bitter almonds, 4-20 parts of cornus officinalis, 6-20 parts of platycodon grandiflorum, 10-22 parts of mulberry, 18-36 parts of white fungus, 5-18 parts of polygonatum, 5-18 parts of wolfberry, 5-18 parts of raw rehmannia root, and 5-18 parts of thunbergia bulb.

[0028] In some embodiments, the compound reed root lung-clearing composition IV comprises, by weight: 45 parts of reed root, 11 parts of momordica grosvenori, 26 parts of bamboo juice, 20 parts of fried bitter almonds, 20 parts of cornus officinalis, 20 parts of platycodon, 22 parts of mulberry, 36 parts of tremella, 18 parts of polygonatum, 18 parts of wolfberry, 18 parts of raw rehmannia, and 18 parts of thunbergia bulb.

[0029] In some embodiments, the compound reed root lung-clearing composition IV is composed of the above components.

[0030] The compound reed root lung-clearing composition of the present invention is mainly suitable for treating lung distension caused by lung failure to clear and purge, and kidney failure to absorb and nourish, lung and kidney yin deficiency, virtual fire inflammation, burning of body fluids, lung dryness and sticky phlegm, and difficulty in coughing and vomiting; in addition, lung and kidney yin deficiency, over time affecting yang, both yin and yang deficiency, kidney failure to absorb and nourish, shortness of breath, lack of breath, and severe wheezing when moving.

[0031] The above-mentioned compound reed root lung-clearing compositions I to IV of the present invention can be effectively used for the treatment of lung and kidney yin deficiency syndrome in the stable stage of chronic obstructive pulmonary disease. From the perspective of therapeutic effect, the compound reed root lung-clearing composition I is superior to the compound reed root lung-clearing compositions II to IV.

[0032] In a second aspect, the present invention provides a compound reed root lung-clearing preparation, which is prepared using the above-mentioned compound reed root lung-clearing compositions I to IV as raw materials and, optionally, conventional excipients used for dosage form preparation. The compound reed root lung-clearing preparation can be in the form of granules, tablets, capsules, pills, powders, syrups, oral solutions, oral suspensions, oral emulsions, mixtures, decoctions, lozenges, fluid extracts, or extracts.

[0033] The third aspect of the present invention provides a method for preparing a compound reed root lung-clearing preparation, which is one of the following methods.

[0034] Method I, which uses the above-mentioned compound reed root lung-clearing composition I as raw material, and the preparation method comprises the following steps:

[0035] (1) Taking the prescribed amount of reed root, white fungus, polygonatum, and wolfberry, adding deionized water for extraction, the extraction temperature is 80-120° C., the amount of water added is 10-15 times the total weight of the reed root, white fungus, polygonatum, and wolfberry, the extraction time is 1-4 hours, filtering, and concentrating the filtrate under reduced pressure at 50-60° C. to an extract with a specific gravity of 1.12-1.21, adding ethanol to an alcohol content of 70-90% (V / V), and standing at 4-7° C. for 6-18 hours, pouring out the supernatant, and drying the lower precipitate under reduced pressure to obtain extract A, recovering ethanol from the supernatant under reduced pressure, concentrating to an extract with a specific gravity of 1.11-1.21, and spray drying to obtain extract B;

[0036] (2) Take the prescribed amount of Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds, add deionized water for extraction, the extraction temperature is 80-120°C, the amount of water added is 10-15 times the weight of Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds, the extraction time is 1-4 hours, filter, and concentrate the filtrate under reduced pressure to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 30-50% (V / V), and let it stand at 4-7°C for 6-18 hours, pour out the supernatant, discard the lower precipitate, and recover ethanol from the supernatant under reduced pressure, concentrate it to an extract with a specific gravity of 1.11-1.21, and spray dry to obtain Extract C;

[0037] (3) Extract the prescribed amount of Momordica grosvenori and mulberry with deionized water at a temperature of 80-120° C. The amount of water added is 10-15 times the total weight of the Momordica grosvenori and mulberry, and the extraction time is 1-4 h. Filter and concentrate the filtrate to an extract with a specific gravity of 1.11-1.21, and spray dry to obtain extract D.

[0038] (4) Take extract A, add 8 to 12 times the weight of extract A in deionized water to dissolve it, and use it as solution a for later use; take extract B, extract C, and extract D, mix them evenly, and evenly spray solution a and the prescribed amount of bamboo juice to prepare granules.

[0039] Method II uses the above-mentioned compound reed root lung-clearing composition II as raw material, and its preparation method is the same as the above-mentioned preparation method I, except that the polygonatum in step (1) is replaced by the prescribed amount of raw rehmannia.

[0040] Method III uses the above-mentioned compound reed root lung-clearing composition III as raw material, and its preparation method is the same as the above-mentioned preparation method I, except that the prescribed amount of raw Rehmannia root is increased in step (1); and bamboo juice is not used in step (4).

[0041] Method IV uses the above-mentioned compound reed root lung-clearing composition IV as raw material, and its preparation method is the same as the above-mentioned preparation method I, except that the prescribed amount of raw Rehmannia root is increased in step (1); and the prescribed amount of Fritillaria thunbergii is increased in step (2).

[0042] In the above preparation methods I-IV, in step (1):

[0043] In some embodiments, the extraction temperature is 100° C.; the extraction time is 2 to 2.5 hours;

[0044] In some embodiments, deionized water extraction can be repeated, with a total of 2 extractions;

[0045] In some embodiments, ethanol is added to an alcohol content of 80% (V / V);

[0046] In some embodiments, the temperature is 4-7°C for 12 hours.

[0047] In the above preparation method, in step (2):

[0048] In some embodiments, the extraction temperature is 100° C. and the extraction time is 2 h;

[0049] In some embodiments, the extraction with deionized water can be repeated, with a total number of extractions of 2 times;

[0050] In some embodiments, ethanol is added to an alcohol content of 40% (V / V);

[0051] In some embodiments, the temperature is 4-7°C for 12 hours.

[0052] In the above preparation method, in step (3):

[0053] In some embodiments, the extraction temperature is 100° C.; the extraction time is 2 h;

[0054] In some embodiments, deionized water extraction can be repeated, with a total of 2 extractions;

[0055] In the above preparation method, in step (4):

[0056] In some embodiments, the amount of deionized water added to dissolve the extract A is 10 times the weight of the extract A.

[0057] The present invention also provides another preparation method V of the compound reed root lung-clearing preparation, which adopts a combined decoction method, and the preparation method comprises:

[0058] Take the above-mentioned compound reed root lung-clearing compositions I to IV, add deionized water for extraction, the extraction temperature is 80-120° C., the amount of water added is 10-15 times the total weight of the composition, the extraction time is 1-4 hours, filter, and concentrate the filtrate under reduced pressure at 50-60° C. to an extract with a specific gravity of 1.12-1.21, spray dry, and obtain an extract.

[0059] In some embodiments, the extraction temperature is 100° C. and the extraction time is 2 to 2.5 hours.

[0060] In some embodiments, the extraction with deionized water can be repeated, with a total of 2 extractions.

[0061] In a fourth aspect, the present invention provides the use of one or more of the above-mentioned compound reed root lung-clearing compositions I to IV, the above-mentioned compound reed root lung-clearing preparation, or the compound reed root lung-clearing preparation prepared by the above-mentioned preparation method in the preparation of a medicament for treating chronic obstructive pulmonary disease, particularly chronic obstructive pulmonary disease with lung and kidney yin deficiency syndrome.

[0062] Beneficial effects

[0063] The invention has abundant raw materials and a scientific and advanced preparation method. The compound reed root lung-clearing composition preparation is effectively used to treat chronic obstructive pulmonary disease, especially has a good therapeutic effect on lung and kidney yin deficiency syndrome in the stable stage of chronic obstructive pulmonary disease, and is an innovation in the medication of chronic obstructive pulmonary disease. BRIEF DESCRIPTION OF THE DRAWINGS

[0064] Figure 1 The HE staining images (100×) of the lung tissues of the rats in each group in Test Example 1 of the present invention are shown; wherein, A is the normal group, B is the model group, C is the doxofylline group, D is the Compound Reed Root Qingfei Granules group ①, E is the Compound Reed Root Qingfei Granules group ②, F is the Compound Reed Root Qingfei Granules group ③, G is the Compound Reed Root Qingfei Granules group ④, H is the Compound Reed Root Qingfei Combined Decoction Granules group ⑤, and I is the Compound Reed Root Qingfei Combined Decoction Granules group ⑥. DETAILED DESCRIPTION

[0065] The specific embodiments of the present invention are further described in detail below with reference to the examples.

[0066] Example 1

[0067] In this example, compound reed root lung-clearing granules were prepared using the compound reed root lung-clearing composition I-1 as a raw material.

[0068] The compound reed root lung-clearing composition I-1 is composed of 20g of reed root, 5g of momordica grosvenori, 7g of bamboo juice, 4g of stir-fried bitter almonds, 4g of cornus officinalis, 6g of platycodon, 10g of mulberry, 18g of tremella, 5g of polygonatum, and 5g of wolfberry.

[0069] The preparation method of the compound reed root lung-clearing granules is as follows:

[0070] (1) Take reed root, white fungus, polygonatum, and wolfberry, add deionized water and extract twice, the extraction temperature is 100° C., the extraction number is 2 times, the amount of water added each time is 10-15 times the total weight of reed root, white fungus, polygonatum, and wolfberry, the two extraction times are 2.5 hours and 2 hours respectively, filter, combine the filtrate, concentrate under reduced pressure at 50-60° C. to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 80% (V / V), let it stand at 4-7° C. for 12 hours, pour out the supernatant, and dry the lower precipitate under reduced pressure to obtain extract A, recover ethanol from the supernatant under reduced pressure, concentrate to an extract with a specific gravity of 1.11-1.21, spray dry, and obtain extract B;

[0071] (2) Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds were extracted twice with deionized water at 100°C for 2 extractions, with the amount of water added each time being 10-15 times the weight of Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds. The extraction time was 2 hours. The extracts were filtered, the filtrates were combined, and the extracts were concentrated under reduced pressure to a specific gravity of 1.12-1.21. Ethanol was added to a concentration of 40% (V / V) of alcohol. The extracts were allowed to stand at 4-7°C for 12 hours. The supernatant was poured out, and the lower precipitate was discarded. The supernatant was decompressed to recover ethanol and concentrated to a specific gravity of 1.11-1.21. The extracts were spray-dried to obtain Extract C.

[0072] (3) Extracting Momordica grosvenori and Morus alba with deionized water twice at 100°C for 2 times, with the amount of water added each time being 10-15 times the total weight of Momordica grosvenori and Morus alba for 2 hours. Filtering, combining the filtrates, concentrating to an extract with a specific gravity of 1.11-1.21, and spray drying to obtain Extract D.

[0073] (4) Take extract A, add deionized water 10 times the weight of extract A to dissolve it, and use it as solution a for later use; take extract B, extract C, and extract D, mix them evenly, and evenly spray solution a and bamboo juice to prepare granules, and add conventional excipients for dosage form preparation to prepare compound reed root lung-clearing granules.

[0074] Example 2

[0075] In this example, compound reed root lung-clearing granules ① were prepared using the compound reed root lung-clearing composition I-2 as a raw material.

[0076] The compound reed root lung-clearing composition I-2 is composed of 45g reed root, 11g momordica grosvenori, 26g bamboo juice, 20g fried bitter almond, 20g cornus officinalis, 20g platycodon, 22g mulberry, 36g white fungus, 18g polygonatum, and 18g wolfberry.

[0077] The preparation method of compound reed root lung-clearing granules ① is as follows:

[0078] (1) Take reed root, white fungus, polygonatum, and wolfberry, add deionized water and extract twice, the extraction temperature is 100° C., the extraction number is 2 times, the amount of water added each time is 10-15 times the total weight of reed root, white fungus, polygonatum, and wolfberry, the two extraction times are 2.5 hours and 2 hours respectively, filter, combine the filtrate, concentrate under reduced pressure at 50-60° C. to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 80% (V / V), let it stand at 4-7° C. for 12 hours, pour out the supernatant, and dry the lower precipitate under reduced pressure to obtain extract A, recover ethanol from the supernatant under reduced pressure, concentrate to an extract with a specific gravity of 1.11-1.21, spray dry, and obtain extract B;

[0079] (2) Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds were extracted twice with deionized water at 100°C for 2 extractions, with the amount of water added each time being 10-15 times the weight of Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds. The extraction time was 2 hours. The extracts were filtered, the filtrates were combined, and the extracts were concentrated under reduced pressure to a specific gravity of 1.12-1.21. Ethanol was added to a concentration of 40% (V / V) of alcohol. The extracts were allowed to stand at 4-7°C for 12 hours. The supernatant was poured out, and the lower precipitate was discarded. The supernatant was decompressed to recover ethanol and concentrated to a specific gravity of 1.11-1.21. The extracts were spray-dried to obtain Extract C.

[0080] (3) Extracting Momordica grosvenori and Morus alba with deionized water twice at 100°C for 2 times, with the amount of water added each time being 10-15 times the total weight of Momordica grosvenori and Morus alba for 2 hours. Filtering, combining the filtrates, concentrating to an extract with a specific gravity of 1.11-1.21, and spray drying to obtain Extract D.

[0081] (4) Take extract A, add deionized water 10 times the weight of extract A to dissolve it, and use it as solution a for later use; take extract B, extract C, and extract D, mix them evenly, and evenly spray solution a and bamboo juice to prepare granules, and add conventional excipients for dosage form preparation to prepare compound reed root lung-clearing granules①.

[0082] Example 3

[0083] In this example, compound reed root lung-clearing granules were prepared using the compound reed root lung-clearing composition I-3 as a raw material.

[0084] The compound reed root lung-clearing composition I-3 is composed of 32g of reed root, 8g of momordica grosvenori, 17g of bamboo juice, 12g of stir-fried bitter almonds, 12g of cornus officinalis, 13g of platycodon, 16g of mulberry, 27g of tremella, 11g of polygonatum, and 12g of wolfberry.

[0085] The preparation method of the compound reed root lung-clearing granules is as follows:

[0086] (1) Take reed root, white fungus, polygonatum, and wolfberry, add deionized water and extract twice, the extraction temperature is 100° C., the extraction number is 2 times, the amount of water added each time is 10-15 times the total weight of reed root, white fungus, polygonatum, and wolfberry, the two extraction times are 2.5 hours and 2 hours respectively, filter, combine the filtrate, concentrate under reduced pressure at 50-60° C. to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 80% (V / V), let it stand at 4-7° C. for 12 hours, pour out the supernatant, and dry the lower precipitate under reduced pressure to obtain extract A, recover ethanol from the supernatant under reduced pressure, concentrate to an extract with a specific gravity of 1.11-1.21, spray dry, and obtain extract B;

[0087] (2) Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds were extracted twice with deionized water at 100°C for 2 extractions, with the amount of water added each time being 10-15 times the weight of Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds. The extraction time was 2 hours. The extracts were filtered, the filtrates were combined, and the extracts were concentrated under reduced pressure to a specific gravity of 1.12-1.21. Ethanol was added to a concentration of 40% (V / V) of alcohol. The extracts were allowed to stand at 4-7°C for 12 hours. The supernatant was poured out, and the lower precipitate was discarded. The supernatant was decompressed to recover ethanol and concentrated to a specific gravity of 1.11-1.21. The extracts were spray-dried to obtain Extract C.

[0088] (3) Extracting Momordica grosvenori and Morus alba with deionized water twice at 100°C for 2 times, with the amount of water added each time being 10-15 times the total weight of Momordica grosvenori and Morus alba for 2 hours. Filtering, combining the filtrates, concentrating to an extract with a specific gravity of 1.11-1.21, and spray drying to obtain Extract D.

[0089] (4) Take extract A, add deionized water 10 times the weight of extract A to dissolve it, and use it as solution a for later use; take extract B, extract C, and extract D, mix them evenly, and evenly spray solution a and bamboo juice to prepare granules, and add conventional excipients for dosage form preparation to prepare compound reed root lung-clearing granules.

[0090] Example 4

[0091] In this example, compound reed root lung-clearing granules were prepared using the compound reed root lung-clearing composition I-4 as a raw material.

[0092] The compound reed root lung-clearing composition I-4 is composed of 20g of reed root, 11g of momordica grosvenori, 17g of bamboo juice, 12g of stir-fried bitter almonds, 20g of cornus officinalis, 6g of platycodon, 10g of mulberry, 36g of tremella, 18g of polygonatum, and 12g of wolfberry.

[0093] The preparation method of the compound reed root lung-clearing granules is as follows:

[0094] (1) Take reed root, white fungus, polygonatum, and wolfberry, add deionized water and extract twice, the extraction temperature is 100° C., the extraction number is 2 times, the amount of water added each time is 10-15 times the total weight of reed root, white fungus, polygonatum, and wolfberry, the two extraction times are 2.5 hours and 2 hours respectively, filter, combine the filtrate, concentrate under reduced pressure at 50-60° C. to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 80% (V / V), let it stand at 4-7° C. for 12 hours, pour out the supernatant, and dry the lower precipitate under reduced pressure to obtain extract A, recover ethanol from the supernatant under reduced pressure, concentrate to an extract with a specific gravity of 1.11-1.21, spray dry, and obtain extract B;

[0095] (2) Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds were extracted twice with deionized water at 100°C for 2 extractions, with the amount of water added each time being 10-15 times the weight of Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds. The extraction time was 2 hours. The extracts were filtered, the filtrates were combined, and the extracts were concentrated under reduced pressure to a specific gravity of 1.12-1.21. Ethanol was added to a concentration of 40% (V / V) of alcohol. The extracts were allowed to stand at 4-7°C for 12 hours. The supernatant was poured out, and the lower precipitate was discarded. The supernatant was decompressed to recover ethanol and concentrated to a specific gravity of 1.11-1.21. The extracts were spray-dried to obtain Extract C.

[0096] (3) Extracting Momordica grosvenori and Morus alba with deionized water twice at 100°C for 2 times, with the amount of water added each time being 10-15 times the total weight of Momordica grosvenori and Morus alba for 2 hours. Filtering, combining the filtrates, concentrating to an extract with a specific gravity of 1.11-1.21, and spray drying to obtain Extract D.

[0097] (4) Take extract A, add deionized water 10 times the weight of extract A to dissolve it, and use it as solution a for later use; take extract B, extract C, and extract D, mix them evenly, and evenly spray solution a and bamboo juice to prepare granules, and add conventional excipients for dosage form preparation to prepare compound reed root lung-clearing granules.

[0098] Example 5

[0099] In this example, compound reed root lung-clearing granules ② were prepared using compound reed root lung-clearing composition II as a raw material.

[0100] The compound reed root lung-clearing composition II is composed of 45g reed root, 11g momordica grosvenori, 26g bamboo juice, 20g fried bitter almond, 20g cornus officinalis, 20g platycodon, 22g mulberry, 36g white fungus, 18g raw rehmannia root, and 18g wolfberry.

[0101] The preparation method of compound reed root lung-clearing granules ② is as follows:

[0102] (1) Take reed root, white fungus, raw rehmannia root, and wolfberry fruit, add deionized water to extract twice, the extraction temperature is 100° C., the extraction number is 2 times, the amount of water added each time is 10-15 times the total weight of reed root, white fungus, raw rehmannia root, and wolfberry fruit, the two extraction times are 2.5 hours and 2 hours respectively, filter, combine the filtrate, concentrate under reduced pressure at 50-60° C. to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 80% (V / V), let it stand at 4-7° C. for 12 hours, pour out the supernatant, and dry the lower precipitate under reduced pressure to obtain extract A, recover ethanol from the supernatant under reduced pressure, concentrate to an extract with a specific gravity of 1.11-1.21, spray dry, and obtain extract B;

[0103] (2) Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds were extracted twice with deionized water at 100°C for 2 extractions, with the amount of water added each time being 10-15 times the weight of Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds. The extraction time was 2 hours. The extracts were filtered, the filtrates were combined, and the extracts were concentrated under reduced pressure to a specific gravity of 1.12-1.21. Ethanol was added to a concentration of 40% (V / V) of alcohol. The extracts were allowed to stand at 4-7°C for 12 hours. The supernatant was poured out, and the lower precipitate was discarded. The supernatant was decompressed to recover ethanol and concentrated to a specific gravity of 1.11-1.21. The extracts were spray-dried to obtain Extract C.

[0104] (3) Extracting Momordica grosvenori and Morus alba with deionized water twice at 100°C for 2 times, with the amount of water added each time being 10-15 times the total weight of Momordica grosvenori and Morus alba for 2 hours. Filtering, combining the filtrates, concentrating to an extract with a specific gravity of 1.11-1.21, and spray drying to obtain Extract D.

[0105] (4) Take extract A, add 10 times the weight of extract A in deionized water to dissolve, and prepare solution a for later use; take extract B, extract C, and extract D, mix them evenly, and evenly spray solution a and bamboo juice to prepare granules, and add conventional excipients for dosage form preparation to prepare compound reed root lung-clearing granules ②.

[0106] Example 6

[0107] In this example, compound reed root lung-clearing granules ③ were prepared using compound reed root lung-clearing composition III as a raw material.

[0108] The compound reed root lung-clearing composition III is composed of 45g reed root, 11g momordica grosvenori, 20g fried bitter almond, 20g cornus officinalis, 20g platycodon, 22g mulberry, 36g tremella, 18g polygonatum, 18g raw rehmannia root, and 18g wolfberry.

[0109] The preparation method of compound reed root lung-clearing granules ③ is as follows:

[0110] (1) Take reed root, white fungus, polygonatum, raw rehmannia root, and wolfberry fruit, add deionized water to extract twice, the extraction temperature is 100°C, the extraction number is 2 times, the amount of water added each time is 10-15 times the total weight of reed root, white fungus, polygonatum, raw rehmannia root, and wolfberry fruit, the two extraction times are 2.5h and 2h respectively, filter, combine the filtrate, concentrate under reduced pressure at 50-60°C to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 80% (V / V), let it stand at 4-7°C for 12h, pour out the supernatant, and dry the lower precipitate under reduced pressure to obtain extract A, recover ethanol from the supernatant under reduced pressure, concentrate to an extract with a specific gravity of 1.11-1.21, spray dry, and obtain extract B;

[0111] (2) Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds were extracted twice with deionized water at 100°C for 2 extractions, with the amount of water added each time being 10-15 times the weight of Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds. The extraction time was 2 hours. The extracts were filtered, the filtrates were combined, and the extracts were concentrated under reduced pressure to a specific gravity of 1.12-1.21. Ethanol was added to a concentration of 40% (V / V) of alcohol. The extracts were allowed to stand at 4-7°C for 12 hours. The supernatant was poured out, and the lower precipitate was discarded. The supernatant was decompressed to recover ethanol and concentrated to a specific gravity of 1.11-1.21. The extracts were spray-dried to obtain Extract C.

[0112] (3) Extracting Momordica grosvenori and Morus alba with deionized water twice at 100°C for 2 times, with the amount of water added each time being 10-15 times the total weight of Momordica grosvenori and Morus alba for 2 hours. Filtering, combining the filtrates, concentrating to an extract with a specific gravity of 1.11-1.21, and spray drying to obtain Extract D.

[0113] (4) Take extract A, add 10 times the weight of extract A in deionized water to dissolve, and prepare solution a for later use; take extract B, extract C, and extract D, mix them evenly, and evenly spray solution a to prepare granules, and add conventional excipients for dosage form preparation to prepare compound reed root lung-clearing granules ③.

[0114] Example 7

[0115] In this example, compound reed root lung-clearing granules ④ were prepared using compound reed root lung-clearing composition IV as a raw material.

[0116] The compound reed root lung-clearing composition IV is composed of 45g reed root, 11g momordica grosvenori, 26g bamboo juice, 20g fried bitter almond, 20g cornus officinalis, 20g platycodon, 22g mulberry, 36g white fungus, 18g polygonatum, 18g wolfberry, 18g raw rehmannia root, and 18g thunbergia bulb.

[0117] The preparation method of compound reed root lung-clearing granules ④ is as follows:

[0118] (1) Take reed root, white fungus, polygonatum, raw rehmannia root, and wolfberry fruit, add deionized water to extract twice, the extraction temperature is 100°C, the extraction number is 2 times, the amount of water added each time is 10-15 times the total weight of reed root, white fungus, polygonatum, raw rehmannia root, and wolfberry fruit, the two extraction times are 2.5h and 2h respectively, filter, combine the filtrate, concentrate under reduced pressure at 50-60°C to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 80% (V / V), let it stand at 4-7°C for 12h, pour out the supernatant, and dry the lower precipitate under reduced pressure to obtain extract A, recover ethanol from the supernatant under reduced pressure, concentrate to an extract with a specific gravity of 1.11-1.21, spray dry, and obtain extract B;

[0119] (2) Platycodon grandiflorum, Cornus officinalis, stir-fried bitter almonds, and Fritillaria thunbergii were extracted twice with deionized water at a temperature of 100°C and a number of extractions of 2 times. The amount of water added each time was 10-15 times the weight of Platycodon grandiflorum, Cornus officinalis, stir-fried bitter almonds, and Fritillaria thunbergii. The extraction time was 2 hours. The extracts were filtered, the filtrates were combined, and the extracts were concentrated under reduced pressure to an extract with a specific gravity of 1.12-1.21. Ethanol was added to an alcohol content of 40% (V / V), and the extracts were allowed to stand at 4-7°C for 12 hours. The supernatant was poured out, and the lower precipitate was discarded. The supernatant was recovered under reduced pressure from the ethanol, and concentrated to an extract with a specific gravity of 1.11-1.21. The extracts were spray-dried to obtain Extract C.

[0120] (3) Extracting Momordica grosvenori and Morus alba with deionized water twice at 100°C for 2 times, with the amount of water added each time being 10-15 times the total weight of Momordica grosvenori and Morus alba for 2 hours. Filtering, combining the filtrates, concentrating to an extract with a specific gravity of 1.11-1.21, and spray drying to obtain Extract D.

[0121] (4) Take extract A, add 10 times the weight of extract A in deionized water to dissolve, and prepare solution a for later use; take extract B, extract C, and extract D, mix them evenly, and evenly spray solution a and bamboo juice to prepare granules, and add conventional excipients for dosage form preparation to prepare compound reed root lung-clearing granules ④.

[0122] Example 8

[0123] In the example, the compound reed root lung-clearing composition I-2 in Example 2 was used as a raw material, and after extraction by a traditional decoction method, a compound reed root lung-clearing prescription preparation, namely, the compound reed root lung-clearing decoction granules ⑤, was prepared.

[0124] The preparation method of the compound reed root lung-clearing decoction granules ⑤ is as follows:

[0125] Take reed rhizome, momordica grosvenori, bamboo juice, stir-fried bitter apricot kernels, cornelian cherry fruit, platycodon root, mulberry, tremella, polygonatum sibiricum, and wolfberry fruit, add deionized water and extract twice. The extraction temperature is 100°C, the extraction times are 2 times, and the water addition amount each time is 10 - 15 times the total weight of reed rhizome, momordica grosvenori, bamboo juice, stir-fried bitter apricot kernels, cornelian cherry fruit, platycodon root, mulberry, tremella, polygonatum sibiricum, and wolfberry fruit. The extraction times are 2.5 h and 2 h in sequence. Filter, combine the filtrates, concentrate under reduced pressure at 50 - 60°C to an extract with a specific gravity of 1.12 - 1.21, spray-dry to obtain the extract of Compound Reed Rhizome and Lung-Clearing Composition I-2, prepare granules, add conventional excipients for dosage form preparation, and make Compound Reed Rhizome and Lung-Clearing Decocted Granules⑤.

[0126] Example 9

[0127] Using the Compound Reed Rhizome and Lung-Clearing Composition I-4 in Example 4 as the raw material in the example, after extraction by the decocting method of traditional decoction, prepare the preparation of Compound Reed Rhizome and Lung-Clearing Prescription, that is, Compound Reed Rhizome and Lung-Clearing Decocted Granules⑥.

[0128] The preparation method of Compound Reed Rhizome and Lung-Clearing Decocted Granules⑥ is as follows: Take reed rhizome, momordica grosvenori, bamboo juice, stir-fried bitter apricot kernels, cornelian cherry fruit, platycodon root, mulberry, tremella, polygonatum sibiricum, and wolfberry fruit, add deionized water and extract twice. The extraction temperature is 100°C, the extraction times are 2 times, and the water addition amount each time is 10 - 15 times the total weight of reed rhizome, momordica grosvenori, bamboo juice, stir-fried bitter apricot kernels, cornelian cherry fruit, platycodon root, mulberry, tremella, polygonatum sibiricum, and wolfberry fruit. The extraction times are 2.5 h and 2 h in sequence. Filter, combine the filtrates, concentrate under reduced pressure at 50 - 60°C to an extract with a specific gravity of 1.12 - 1.21, spray-dry to obtain the extract of Compound Reed Rhizome and Lung-Clearing Composition I-4, prepare granules, add conventional excipients for dosage form preparation, and make Compound Reed Rhizome and Lung-Clearing Decocted Granules⑥.

[0129] Test Example 1

[0130] The present invention has repeatedly studied the curative effects of the preparations obtained in the above-mentioned various examples on rats with chronic obstructive pulmonary disease, and all have achieved satisfactory treatment effects. The relevant research data are as follows:

[0131] 1. Experimental materials

[0132] 1.1 Animals: 108 SPF-grade SD rats, half male and half female, 2 months old, with a body weight of 200 ± 20 g (animal certificate number: NO.110011211105823815, Beijing Vital River Laboratory Animal Technology Co., Ltd.). The license number for the experimental unit's use is: SYXK(Yu)2020 - 0004.

[0133] 1.2 Bacteria: Klebsiella pneumoniae (KP) (strain number: 46114) was purchased from the China Food and Drug Administration (formerly the China Food and Drug Administration) and the concentration of the bacteria was adjusted to 6 × 10 8 CFU (Colony Forming Unit) / ml suspension.

[0134] 1.3 Cigarettes: Hongqiqu brand filter cigarettes provided by Henan China Tobacco Industry Co., Ltd. (flue-cured tobacco type, tar content 11mg, smoke nicotine content 0.9mg, smoke carbon monoxide content 11mg)

[0135] 1.4 Drugs: (1) Doxofylline Tablets (Heilongjiang Fuhe Pharmaceutical Group Co., Ltd., 0.2 g / tablet). Before use, grind the drug into powder and add 5% CMC-Na to prepare a 1.0 mg / ml suspension; (2) Compound Phragmites australis Lung-Clearing Preparations: (i) Compound Phragmites australis Lung-Clearing Granules ① prepared in Example 2 of the present invention (containing 1 g of the decoction piece / g); (ii) Granules ② prepared in Example 5 of the present invention (containing 1 g of the decoction piece / g); (iii) Granules ③ prepared in Example 6 of the present invention (containing 1 g of the decoction piece / g); (iv) Granules ④ prepared in Example 7 of the present invention (containing 1 g of the decoction piece / g); (v) Combined Decoction Granules ⑤ prepared in Example 8 of the present invention (containing 1 g of the decoction piece / g); (vi) Combined Decoction Granules ⑥ prepared in Example 9 of the present invention (containing 1 g of the decoction piece / g).

[0136] 1.5 Reagents: The rat TNF-α enzyme-linked immunosorbent assay kit (E-EL-R2856c) was purchased from Wuhan Elaruite Biotechnology Co., Ltd., and the rat IL-6 enzyme-linked immunosorbent assay kit (550319) was provided by BD Biosciences, Inc., USA. The total superoxide dismutase (T-SOD) assay kit (hydroxylamine method, A001-1-2) and malondialdehyde (MDA) assay kit (TBA method, A003-1-2) were provided by Nanjing Jiancheng Bioengineering Institute. PE anti-rat CD4AntibodV (201507), APC anti-rat CD8a Antibody (200610), FITC anti-rat CD3Antibody (201403), FITC Mouse IgM, K Isotype Ctrl Antibody (401605), APC Mouse IgG2a, K Isotype Ctrl Antibody (400219), and PE Mouse IgG1, K Isotype Ctrl Antibody (400111) were all purchased from Biolegend, USA.

[0137] 1.6 Instruments: IVC-II animal cages (Fengshi Laboratory Animal Equipment Co., Ltd., Suzhou); fumigation chamber (Buxco, USA); WBP unrestrained whole-body plethysmography system (noninvasive respiratory function measurement system) (Buxco, USA); OLYMPUS-DP70 microscope and microphotography system (Olympus, Japan), microplate reader (Thermo, USA), and flow cytometer (BD, USA).

[0138] 2. Experimental Methods

[0139] 2.1 Model preparation

[0140] Except for the normal group, the rat models of stable COPD were established in the other groups using cigarette smoking combined with repeated Klebsiella pneumoniae infection. Smoke exposure was performed using a smoke exposure system at a concentration of 3000 ± 500 ppm. Cigarettes were inhaled twice daily, 40 minutes per inhalation, with at least 3 hours between inhalations, for a total of 8 weeks. At the same time, 6×10 Klebsiella pneumoniae solution was instilled into the nasal cavity. 8 CFU / ml, 0.1ml, once every 5 days.

[0141] 2.2 Grouping and Dosing

[0142] Rats were randomly divided into a normal group, a model group, a compound Lugen Qingfei preparation group (including compound Lugen Qingfei granules ① to ④ and compound Lugen Qingfei combined decoction granules ⑤ to ⑥), and a doxofylline group, with 12 rats in each group. The model was established for 8 weeks. From the 9th week onwards, each group was given 2 ml of normal saline (blank group, model group), 54 mg·kg aminophylline, and 10 mg·kg -1 ·d -1 (aminophylline group), compound reed root lung-clearing granules, compound reed root lung-clearing granules ① to ④ or compound reed root lung-clearing combined decoction granules ⑤ to ⑥ (10.5 g slices·kg -1 ·d -1 , respectively for the corresponding preparation groups) gavage, 2 times d -1 The mice were gavaged at 8:00-9:00 and 17:00-18:00. Body weight was measured weekly to adjust the dosage. The drug was administered for a total of 8 weeks, and samples were collected on the 17th week.

[0143] 2.3 Sampling and index determination

[0144] 2.3.1 General condition: Observe the rat's mental state, activity, fur, secretions, changes in food and water intake, urination and defecation, as well as changes in symptoms and signs such as coughing, wheezing, and weight.

[0145] 2.3.2 Pulmonary function: After modeling (at the end of the 8th week) and before sampling (at the end of the 16th week), the rat unrestrained whole body plethysmography system (WBP) was used to detect the peak expiratory flow (PEF), expiratory flow at 50% tidal volume (EF50), expiratory time (Te), and inspiratory time (Tr) of the rats.

[0146] 2.3.3 Collection of bronchoalveolar lavage fluid (BALF): After the 16th week, anesthesia was performed under 1% sodium pentobarbital 50 mg / kg, the chest cavity was opened, the lungs were removed, the right main bronchus was ligated, the left lung was separated, and the left lung was perfused with 4°C pre-cooled saline. 3 ml of saline was drawn each time and injected into the left lung for lavage. The lavage fluid after withdrawal was filtered through sterile gauze and injected into a centrifuge tube. This process was repeated 3 times until the withdrawal rate reached more than 80%. The samples were centrifuged at 3000 rpm for 10 min, and the supernatant was frozen for later use.

[0147] 2.3.4 Homogenize lung tissue: Take 50 mg of lung tissue, add pre-cooled PBS at a ratio of 1:9, homogenize in a high-speed homogenizer, collect the supernatant, and freeze at -80℃ for later use.

[0148] 2.3.5 Lung tissue pathology: After alveolar lavage fluid was collected from the left lung, it was perfused with 10% neutral formaldehyde for 30 minutes, then placed in formaldehyde solution for perfusion fixation for 72 hours, embedded in paraffin, and sectioned at 4 μm. Conventional HE staining was performed. Alveolar pathological changes were observed under an optical microscope. Eight slides were randomly selected from each group, and six fields of view were randomly observed under a 200x microscope on each slide. A perpendicular "cross" was drawn on each field of view. The size of the "cross" in each field of view was fixed. The total length of the "cross" was measured as L, and the total area of ​​the field of view was S. The number of alveolar septa passing through the "cross" was counted (Ns). The number of alveoli in each field of view was counted (Na). The mean alveolar intercept MLI (μm) = L / Ns and the mean alveolar number per unit area MAN ( / mm) were calculated. 2 )=Na / S. The typical HE staining images of rat lung tissues in each group are shown in Figure 1 .

[0149] 2.3.6 Changes of IL-6 and TNF-a in bronchoalveolar lavage fluid in each group

[0150] Enzyme-linked immunosorbent assay (ELISA) kits were used to detect changes in IL-6 and TNF-α levels in bronchoalveolar lavage fluid: a preliminary test was first performed to select the appropriate dilution ratio of the sample, and then a formal test was performed according to the kit instructions.

[0151] 2.3.7 Changes of T-SOD and MDA in lung homogenates of each group

[0152] T-SOD was detected using a total superoxide dismutase (T-SOD) test kit (hydroxylamine method), and MDA was detected using a malondialdehyde (MDA) assay kit (TBA method), according to the kit instructions.

[0153] 2.3.8 Changes in T lymphocyte subsets in each group

[0154] CD3+CD4+, CD3+CD8+, and CD4+ / CD8+ were measured using flow cytometry. To 100μl of peripheral blood, 1.25μl of PE-labeled CD4, 0.5μl of FICT CD3, and 1.25μl of APC CD8 were added, respectively. Red blood cell lysis buffer was added at a 1:20 ratio, the cells were mixed and centrifuged, and the cells were resuspended and washed in PBS. The cells were then resuspended in 300μl of pre-chilled PBS and analyzed.

[0155] 3. Experimental Results

[0156] 3.1 General

[0157] During the 16-week observation period, the fur of the rats in the blank group was white and shiny, and they were lively and active, with normal food and water intake. The model rats (except the blank group) gradually showed symptoms of dull fur, fatigue and lack of activity from the 4th week. From the 8th week, the model rats generally showed symptoms of mental depression, poor appetite and laziness, and some rats showed symptoms of asthma and phlegm sounds in the throat. The spirits of the rats in the compound reed root lung-clearing preparation group and the doxofylline group improved after medication, and their activity level and food and water intake increased.

[0158] 3.2 Weight changes

[0159] At the end of the 8th week of modeling, the body weight of each modeling group was significantly lower than that of the normal group (P < 0.01). After 8 weeks of treatment, the body weight of the model group rats was still lower than that of the normal group (P < 0.05). The body weight of the doxofylline group and the compound reed root lung-clearing granules groups ①, ③, ④, and ⑤ increased significantly (P < 0.05, P < 0.01). The other groups also showed an upward trend. In order to compare the difference between the traditional decoction method and the step-by-step extraction method used in Example 2, the inventors set up a statistical analysis between the compound reed root lung-clearing granules group ① and the compound reed root lung-clearing granules groups ⑤ and ⑥. The results showed that after treatment, the body weight of the compound reed root lung-clearing granules groups ⑤ and ⑥ was significantly lower than that of the group ①, indicating that the step-by-step extraction process is superior to the traditional decoction process. See Table 1.

[0160] Table 1 Body weight changes of rats in each group (g, )

[0161]

[0162] Note: n=12, n is the number of rats. Compared with the normal group, **P<0.01, * P<0.05; compared with the model group, ## P < 0.01, # P < 0.05;

[0163] Compared with group ①, ▲ P<0.05.

[0164] 3.3 Lung function

[0165] Pulmonary function is a key indicator of airflow limitation, and decreased lung function indicates worsening airflow limitation. The results of this study showed that both the model group and the control group experienced a decline in lung function at week 8, with significant decreases in PEF and EF50 (P < 0.05). After 8 weeks of treatment, the PEF and EF50 in the model group were still lower than those in the normal group (P < 0.01); the PEF in the Compound Lugen Qingfei Granules groups ①, ②, and ③ were significantly higher than those in the model group (P < 0.01, P < 0.05); the EF50 in the Compound Lugen Qingfei Granules groups ①, ②, and ④ were significantly higher than those in the model group (P < 0.05, P < 0.01); the PEF and EF50 in the doxofylline group were also significantly higher than those in the model group (P < 0.05, P < 0.01), and the PEF and EF50 in the Compound Lugen Qingfei Granules group ① were significantly higher than those in the doxofylline group (P < 0.01); there were significant differences in the PEF and EF50 between the Compound Lugen Qingfei Granules group ① and the Compound Lugen Qingfei Hejian Granules groups ⑤ and ⑥ (P < 0.05), as shown in Table 2.

[0166] The results showed that Compound Lugen Qingfei Granules ①, ②, ③, and ④ significantly improved the degree of airflow limitation in COPD rats. The statistical difference between Compound Lugen Qingfei Granules group ① and Compound Lugen Qingfei Decoction Granules groups ⑤ and ⑥ also showed that the step-by-step extraction process was superior to the traditional decoction process.

[0167] Table 2 Changes of PEF and EF50 of lung function in rats in each group

[0168]

[0169] Note: n=12. Compared with the normal group, ** P < 0.01, * P<0.05; compared with the model group, ## P < 0.01, # P<0.05; compared with the doxofylline group, Δ P<0.05; compared with group ①, ▲ P<0.05.

[0170] 3.4 Lung Tissue Pathology

[0171] The main pathological changes of COPD are alveolar wall rupture and fusion, and alveolar cavity enlargement. The results of this study showed that the lung tissue structure of rats in the normal group was basically normal, with clear respiratory bronchioles and alveolar sac structures, normal airway structure, intact alveolar wall structure, and no obvious inflammatory cell infiltration in the alveolar cavity (see Figure 1 In the model group, the alveolar walls of the rat lungs were partially ruptured and fused, the alveolar cavity was enlarged, inflammatory cells infiltrated, and the small blood vessel walls were thickened (see Figure 1In the doxofylline group, no obvious mucus secretion was observed in the airway cavity, but obvious inflammatory cell infiltration was observed. The alveolar wall was partially broken and fused, the alveolar cavity was expanded, and obvious inflammatory cell infiltration was observed in the alveolar cavity (see Figure 1 In the Fufang Lugen Qingfei Preparation group, there was no obvious rupture and fusion of the alveolar wall, a small amount of inflammatory cell infiltration in the alveolar cavity, or a small amount of inflammatory cell infiltration was seen, and the airway wall thickening was not obvious (see Figure 1 (DI).

[0172] Compared with the normal group, the mean alveolar number per unit area (MAN) in the model group was significantly decreased (P < 0.01), and the mean alveolar intercept (MLI) was significantly increased (P < 0.01); compared with the model group, MAN in the Compound Lugen Qingfei Granules ①, ③, ④ groups and the doxofylline group were significantly increased (P < 0.05, P < 0.01), and MLI in the Compound Lugen Qingfei Granules ①, ③ and doxofylline groups were significantly decreased (P < 0.05, P < 0.01), and MAN in the Compound Lugen Qingfei Granules ① group was significantly decreased compared with the doxofylline group (P < 0.05); MAN and MLI in the Compound Lugen Qingfei Granules ① group were significantly different from those in the Compound Lugen Qingfei Hejian Granules ⑤ and ⑥ groups (P < 0.05), as shown in Table 3.

[0173] The results showed that Compound Lugen Qingfei Granules ①, ③, and ④, as well as doxofylline, significantly improved lung tissue damage in COPD rats, with Compound Lugen Qingfei Granule ① being superior to doxofylline. The statistically significant differences between Compound Lugen Qingfei Granule ① and Compound Lugen Qingfei Combined Decoction Granules ⑤ and ⑥ also demonstrated that the step-by-step extraction process was superior to the traditional combined decoction process.

[0174] Table 3 Average number of alveoli and average alveolar intercept of rats in each group

[0175]

[0176] Note: n=12. Compared with the normal group, ** P<0.01; compared with the model group, ## P < 0.01, # P<0.05; compared with the doxofylline group, Δ P<0.05; compared with group ①, ▲ P<0.05.

[0177] 3.5 Changes of T-SOD and MDA in lung homogenates of rats in each group

[0178] Oxidation / antioxidation imbalance is one of the main pathological mechanisms of COPD. Malondialdehyde (MDA) is a typical oxidant that aggravates the progression of COPD. Total superoxide dismutase (T-SOD) is an important antioxidant enzyme that can inhibit oxidative stress. The results of this study showed that compared with the normal group, the MDA level of lung homogenate in the model group rats was increased (P < 0.01), and the T-SOD activity was decreased (P < 0.01); compared with the model group, the MDA levels of the Compound Lugen Qingfei Granules ① and doxofylline groups were decreased (P < 0.05, P < 0.01), and the T-SOD activity of the Compound Lugen Qingfei Granules ①, ② and doxofylline groups were increased (P < 0.05, P < 0.01), and the T-SOD activity of the doxofylline group was significantly increased (P < 0.01); there were significant differences in MDA and T-SOD between the Compound Lugen Qingfei Granules ① group and the Compound Lugen Qingfei Hejian Granules ⑤ and ⑥ groups (P < 0.05), see Table 4.

[0179] The results showed that Compound Lugen Qingfei Granules ① and ② had good antioxidant effects. The statistical difference between Compound Lugen Qingfei Granules group ① and Compound Lugen Qingfei Decoction Granules groups ⑤ and ⑥ also showed that the step-by-step extraction process was superior to the traditional decoction process.

[0180] Table 4 Changes of T-SOD and MDA in lung homogenates of rats in each group

[0181]

[0182] Note: n=12. Compared with the normal group, ** P < 0.01, * P<0.05; compared with the model group, # P<0.05; compared with group ①, ▲ P<0.05.

[0183] 3.6 Changes of IL-6 and TNF-α in BALF of rats in each group

[0184] Inflammatory response is another major pathological mechanism of COPD, and interleukin (IL) factors are important mediators of inflammatory response. Compared with the normal group, the IL-6 and TNF-α levels in the BALF of the model group were significantly increased (P < 0.01). Compared with the model group, the IL-6 and TNF-α levels in the BALF of the Compound Lugen Qingfei Granules groups ①, ②, and ③ were significantly decreased (P < 0.01, P < 0.05), and the IL-6 level in the BALF of the Compound Lugen Qingfei Granules group ④ was significantly decreased (P < 0.01, P < 0.05). The IL-6 and TNF-α levels in the doxofylline group were decreased (P < 0.01). There were significant differences in the IL-6 and TNF-α levels between the Compound Lugen Qingfei Granules group ① and the Compound Lugen Qingfei Hejian Granules groups ⑤ and ⑥ (P < 0.05), as shown in Table 5.

[0185] The results showed that Compound Lugen Qingfei Granules ①, ②, ③, and ④ had significant anti-inflammatory effects. The statistical difference between Compound Lugen Qingfei Granules Group ① and Compound Lugen Qingfei Combined Decoction Granules Groups ⑤ and ⑥ also showed that the step-by-step extraction process was superior to the traditional combined decoction process.

[0186] Table 5 Changes of IL-6 and TNF-a levels in BALF of rats in each group

[0187]

[0188]

[0189] Note: n=12, compared with the normal group, ** P < 0.01, * P<0.05; compared with the model group, ## P < 0.01, # P<0.05; compared with group ①, ▲ P<0.05.

[0190] 3.7 Changes in T lymphocyte typing in rats in each group

[0191] Decreased immune function plays an important role in the development of COPD and promotes the progression of the disease. T lymphocyte subsets are the main indicators of the body's immune function. Compared with the normal group, the model group had a significantly decreased CD3 + CD4 + Decreased expression of CD3 + CD8 + Increased, CD4 + / CD8 + Compared with the model group, the CD3 + CD4 + The expression of CD3 + CD8 + There was a downward trend (P<0.05, P<0.01), the CD4 + / CD8 + There was an increasing trend (P<0.01); CD3 + CD4 + 、CD3 + CD8 + 、CD4 + / CD8 + The expression levels were significantly different from those in the ⑤ and ⑥ groups of the compound reed root clearing lung decoction granules (P < 0.05), as shown in Table 6.

[0192] The results showed that Compound Lugen Qingfei Granules ①, ②, ③, ④, and ⑤ had certain immunomodulatory effects and could improve the body's immune function to a certain extent. The statistical difference between Compound Lugen Qingfei Granules Group ① and Compound Lugen Qingfei Decoction Granules Groups ⑤ and ⑥ also showed that the step-by-step extraction process was superior to the traditional decoction process.

[0193] Table 6 Changes of T lymphocyte typing in rats in each group

[0194]

[0195] Note: n=12, compared with the normal group, ** P<0.01; compared with the model group, ## P < 0.01, # P<0.05; compared with group ①, ▲ P<0.05.

[0196] 4. Conclusion

[0197] The compound reed root lung-clearing composition preparation and doxofylline of the present invention can significantly improve the lung function and lung tissue pathology of COPD rats, and the compound reed root lung-clearing granules ① are particularly significant in improving the lung function and alveolar structure; the compound reed root lung-clearing granules ① can reduce the MDA level in the lung homogenate and the IL-6 level in the BALF of COPD rats, and have good anti-inflammatory and antioxidant effects; the compound reed root lung-clearing granules ① can increase the expression of CD4+T lymphocytes in the blood of COPD rats, and have good immunoregulatory effects; doxofylline is a commonly used clinical drug that can increase the activity of the antioxidant enzyme SOD and reduce the IL-6 level in the BALF. The compound reed root lung-clearing granules ① have comparable anti-inflammatory and antioxidant effects and are even more significant than doxofylline in improving the alveolar structure.

[0198] In summary, Compound Lugen Qingfei Granules① exhibited potent anti-inflammatory and antioxidant effects, improving lung function and lung tissue pathological damage in COPD rats. It is effective for treating COPD and improving airflow limitation, alleviating lung tissue damage, inhibiting pulmonary inflammation and oxidative stress, and enhancing immune function. Furthermore, it offers advantages such as a small dosage form, convenient portability, clearly defined components, and reasonable price. This represents an innovation in Traditional Chinese Medicine treatment for stable COPD and possesses high practical value.

[0199] Compound Lugen Qingfei Granules ② to ④ and Compound Lugen Qingfei Decoction Granules ⑤ to ⑥ can also improve the lung function and lung tissue pathology of COPD rats to a certain extent, reduce the MDA level in lung homogenate and IL-6 level in BALF of COPD rats, increase the expression of CD4+T lymphocytes in the blood of COPD rats, increase the activity of antioxidant enzyme SOD, and reduce the IL-6 level in BALF, but their improvement effect on COPD symptoms is significantly weaker than that of Compound Lugen Qingfei Granule ①.

[0200] Test Example 2

[0201] This test example provides a compound reed root lung-clearing composition for the treatment of patients with chronic obstructive pulmonary disease (COPD) with lung and kidney yin deficiency syndrome. The following are typical cases.

[0202] Case 1

[0203] The patient, Mr. Xia, male, 56 years old, from Xinyang, Henan Province, came to the hospital for treatment on June 26, 2022.

[0204] Chief complaint: intermittent chest tightness, shortness of breath, cough, and sputum for more than 5 years.

[0205] Current symptoms: The patient is conscious but slightly less energetic, with chest tightness, shortness of breath, cough, and sputum. The sputum is yellow-white and sticky and difficult to cough up. The patient has a good appetite, poor sleep, slightly dry stool, and frequent urination, mainly at night (an average of 7-8 times per night), which is slightly yellowish in color.

[0206] The patient was treated with 7 doses of the compound reed root lung-clearing composition I-2 in Example 2, 1 dose per day.

[0207] Second visit: The patient reported significant improvement in nocturia (3-4 times per night on average), chest tightness, and wheezing, with occasional cough and sputum, which was white, thin, and easy to cough up. He continued to take 7 doses, one dose per day.

[0208] Third visit: All symptoms have improved compared to before. The patient is required to take this prescription in the form of pills (water pills) for treatment, one dose per day.

[0209] During the 3-month follow-up, the patient's cough and sputum disappeared, chest tightness and shortness of breath occurred occasionally after activities, and nocturia occurred 1-2 times per night.

[0210] Case 2

[0211] The patient, Mr. Tao, male, 50 years old, from Fuyang, Anhui, came to the hospital for treatment on April 20, 2022.

[0212] Chief complaint: intermittent chest tightness, shortness of breath, cough, and sputum for more than 2 years, diagnosed as COPD at a local hospital.

[0213] Current symptoms: clear consciousness, poor spirits, intermittent chest tightness and shortness of breath, aggravated by activity, cough and sputum, the sputum is yellow and sticky and difficult to cough up, general fatigue, obvious sweating, good appetite and sleep, thick stools, and yellow urine.

[0214] The patients were treated with the compound reed root lung-clearing composition II in Example 5 for one week, one dose per day.

[0215] Second visit: The patient's cough and sputum symptoms improved compared to the previous period, but chest tightness and shortness of breath remained severe after exercise, and general fatigue and sweating did not improve. The stool was loose and frequent, with 2-3 bowel movements per day. The patient was treated with 5 doses of the compound reed root lung-clearing composition I-2 in Example 2, 1 dose per day.

[0216] After taking the medicine, the patient was re-examined and the symptoms of chest tightness and asthma were significantly improved compared with before, the activity endurance was significantly increased, the bowel movements returned to normal, and the urination was regular.

[0217] The patient took 45 doses of the compound reed root lung-clearing composition I-2 in Example 2, and the patient's symptoms improved. During the 3-month follow-up, no recurrence of the symptoms was observed.

[0218] Case 3

[0219] The patient, Mr. Li, male, 59 years old, from Zhengzhou, Henan Province, came to the hospital for treatment on April 5, 2022.

[0220] Chief complaint: intermittent chest tightness and shortness of breath for more than 3 years, accompanied by cough and sputum, the sputum is yellow-white and sticky and difficult to cough up, poor appetite, shallow sleep, dry stool, and yellow urine.

[0221] Current symptoms: clear consciousness, poor spirits, chest tightness, shortness of breath aggravated by activity and lying down.

[0222] After one week of treatment with the compound reed root lung-clearing composition III in Example 6, one dose per day, the chest tightness and shortness of breath symptoms improved, but coughing up yellow sputum, chest tightness, shortness of breath, loose stools, and frequent urination were still present. The compound reed root lung-clearing composition I-2 in Example 2 was given for 7 doses, one dose per day.

[0223] Second diagnosis: After taking combination I-2 for 7 days, the amount of sputum was significantly reduced, and it was thin and easy to cough up. The chest tightness and shortness of breath after activities disappeared, and the sleep was significantly improved.

[0224] The patient continued to take 30 doses of the compound reed root lung-clearing combination 1-2 and was followed up for 3 months. The symptoms were stable and there was no recurrence.

[0225] Case 4

[0226] The patient, Mr. Li, male, 56 years old, from Zhumadian, Henan Province, first visited the hospital on March 2, 2022.

[0227] Chief complaint: Intermittent cough and sputum for more than 5 years, aggravated by chest tightness and shortness of breath for half a year.

[0228] Current symptoms: conscious but poor spirits, cough and expectoration of yellow, sticky, and difficult-to-cough sputum, chest tightness and shortness of breath aggravated by activity and lying down, accompanied by general fatigue, sweating, poor appetite, poor sleep, dry stools, and frequent urination. Diagnosed with COPD 2 years ago.

[0229] The first visit was to give the compound reed root lung-clearing composition IV of Example 7 for 7 doses, 1 dose per day. After taking it, the cough and sputum symptoms improved significantly, and the amount of sputum was significantly reduced, but the stool was watery, 4-5 times a day, accompanied by abdominal pain. After taking 3 doses and stopping the drug, the stool gradually returned to normal, and the chest tightness and shortness of breath remained the same.

[0230] Second visit: Administer 5 doses of Compound Reed Root Qingfei Composition I-2, 1 dose per day. After taking the medicine, all symptoms improved compared to before, but chest tightness and shortness of breath still occurred occasionally after exercise. After that, Compound Reed Root Qingfei Composition I-2 was continued for 27 doses.

[0231] During the 3-month follow-up, all symptoms disappeared and no recurrence was observed.

[0232] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment, it is not intended to limit the present invention. Any technician familiar with the present profession can make some changes or modifications to equivalent embodiments of equivalent changes using the technical contents disclosed above without departing from the scope of the technical solution of the present invention. However, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are within the scope of the technical solution of the present invention.

Claims

1. A compound reed root lung-clearing preparation for treating chronic obstructive pulmonary disease, which is prepared using the following compound reed root lung-clearing composition I as raw materials, wherein: The compound reed root lung-clearing composition I is composed of the following components in parts by weight: 20-45 parts of reed rhizome, 5-11 parts of momordica grosvenori, 7-26 parts of bamboo juice, 4-20 parts of stir-fried bitter almonds, 4-20 parts of cornus officinalis, 6-20 parts of platycodon grandiflorum, 10-22 parts of mulberry, 18-36 parts of white fungus, 5-18 parts of polygonatum, and 5-18 parts of wolfberry; The preparation method of the compound reed root lung-clearing preparation comprises the following steps: (1) Take the prescribed amount of Phragmites australis, Tremella fuciformis, Polygonatum sibiricum, and Lycium barbarum, add deionized water for extraction, the extraction temperature is 80-120° C., the amount of water added is 10-15 times the total weight of the medicinal materials, the extraction time is 1-4 hours, filter, and concentrate the filtrate under reduced pressure at 50-60° C. to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 70-90% (V / V), and let it stand at 4-7° C. for 6-18 hours, pour out the supernatant, and dry the lower precipitate under reduced pressure to obtain Extract A, recover ethanol from the supernatant under reduced pressure, concentrate to an extract with a specific gravity of 1.11-1.21, and spray dry to obtain Extract B; (2) Take the prescribed amount of Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds, add deionized water for extraction, the extraction temperature is 80-120°C, the amount of water added is 10-15 times the total weight of the medicinal materials, the extraction time is 1-4 hours, filter, and concentrate the filtrate under reduced pressure to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 30-50%, V / V, and let it stand at 4-7°C for 6-18 hours, pour out the supernatant, discard the lower precipitate, and recover ethanol from the supernatant under reduced pressure, concentrate it to an extract with a specific gravity of 1.11-1.21, and spray dry to obtain Extract C; (3) Extract the prescribed amount of Momordica grosvenori and Morus alba with deionized water at 80-120°C, add 10-15 times the total weight of the medicinal materials, and extract for 1-4 hours. Filter and concentrate the filtrate to an extract with a specific gravity of 1.11-1.21, spray dry, and obtain Extract D. (4) Take extract A, add 8 to 12 times the weight of extract A in deionized water to dissolve it, and use it as solution a for later use; take extract B, extract C, and extract D, mix them evenly, and evenly spray solution a and the prescribed amount of bamboo juice to prepare granules.

2. The compound reed root lung-clearing preparation according to claim 1, characterized in that Compound reed root lung-clearing composition I is composed of the following components, in parts by weight: 45 parts of reed root, 11 parts of momordica grosvenori, 26 parts of bamboo sap, 20 parts of stir-fried bitter almonds, 20 parts of cornus officinalis, 20 parts of platycodon, 22 parts of mulberry, 36 parts of tremella, 18 parts of polygonatum, and 18 parts of wolfberry.

3. The compound reed root lung-clearing preparation according to claim 1, further comprising an auxiliary material.

4. The compound reed root lung-clearing preparation according to claim 3, characterized in that The compound reed root lung-clearing preparation is selected from granules, tablets, capsules, pills, powders, syrups, mixtures, and lozenges.

5. The compound reed root lung-clearing preparation according to claim 3, characterized in that The compound reed root lung-clearing preparation is selected from oral solution, oral suspension and oral emulsion.

6. A method for preparing a compound reed rhizome lung-clearing preparation, which uses the compound reed rhizome lung-clearing composition I described in claim 1 or 2 as a raw material, comprising the following steps: (1) Take the prescribed amount of Phragmites australis, Tremella fuciformis, Polygonatum sibiricum, and Lycium barbarum, add deionized water for extraction, the extraction temperature is 80-120° C., the amount of water added is 10-15 times the total weight of the medicinal materials, the extraction time is 1-4 hours, filter, and concentrate the filtrate under reduced pressure at 50-60° C. to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 70-90% (V / V), and let it stand at 4-7° C. for 6-18 hours, pour out the supernatant, and dry the lower precipitate under reduced pressure to obtain Extract A, recover ethanol from the supernatant under reduced pressure, concentrate to an extract with a specific gravity of 1.11-1.21, and spray dry to obtain Extract B; (2) Take the prescribed amount of Platycodon grandiflorum, Cornus officinalis, and stir-fried bitter almonds, add deionized water for extraction, the extraction temperature is 80-120°C, the amount of water added is 10-15 times the total weight of the medicinal materials, the extraction time is 1-4 hours, filter, and concentrate the filtrate under reduced pressure to an extract with a specific gravity of 1.12-1.21, add ethanol to an alcohol content of 30-50%, V / V, and let it stand at 4-7°C for 6-18 hours, pour out the supernatant, discard the lower precipitate, and recover ethanol from the supernatant under reduced pressure, concentrate it to an extract with a specific gravity of 1.11-1.21, and spray dry to obtain Extract C; (3) Extract the prescribed amount of Momordica grosvenori and Morus alba with deionized water at 80-120°C, add 10-15 times the total weight of the medicinal materials, and extract for 1-4 hours. Filter and concentrate the filtrate to an extract with a specific gravity of 1.11-1.21, spray dry, and obtain Extract D. (4) Take extract A, add 8 to 12 times the weight of extract A in deionized water to dissolve it, and use it as solution a for later use; take extract B, extract C, and extract D, mix them evenly, and evenly spray solution a and the prescribed amount of bamboo juice to prepare granules.

7. The preparation method according to claim 6, characterized in that In step (1): The extraction temperature was 100°C; The extraction time is 2 to 2.5 hours; Repeat the extraction with deionized water for a total of 2 extractions; Add ethanol to 80% alcohol content (V / V); let stand at 4-7°C for 12 hours; and / or In step (2): The extraction temperature was 100°C and the extraction time was 2 h; Repeat the extraction with deionized water for a total of 2 extractions; Add ethanol to a concentration of 40% (V / V); Incubate at 4-7°C for 12 hours; and / or In step (3): The extraction temperature was 100°C and the extraction time was 2 h; Repeat the extraction with deionized water for a total of 2 extractions; and / or In step (4): The amount of deionized water added to dissolve the extract A is 10 times the weight of the extract A.

8. Use of the compound reed root lung-clearing preparation according to any one of claims 1 to 5 in the preparation of a medicament for treating chronic obstructive pulmonary disease.

9. The use according to claim 8, characterized in that The chronic obstructive pulmonary disease is chronic obstructive pulmonary disease with lung and kidney yin deficiency syndrome.

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