A traditional Chinese medicine composition for promoting wound healing, a coating agent and a preparation method and application thereof

Abstract

The application discloses a traditional Chinese medicine composition for promoting wound healing, a film coating agent, a preparation method and application thereof. The traditional Chinese medicine composition is prepared from raw medicinal materials and includes the following components in parts by weight: Verbena 3-7 parts, Eupatorium adenophorum 1-5 parts, and water nail 0.5-4 parts. The traditional Chinese medicine composition is prepared from the Verbena, the Eupatorium adenophorum and the water nail in a specific ratio, and the three medicines have a synergistic effect and have a bacteriostatic effect on Escherichia coli and Staphylococcus aureus. The 75% alcohol extract of the three medicines has a significant bacteriostatic effect on the Escherichia coli and the Staphylococcus aureus. The application has the effects of resisting bacteria, reducing inflammation, reducing swelling, stopping bleeding, relieving pain, and the like. The film coating agent has good film forming property and flexibility, appropriate viscosity, good stability, strong adhesion to the skin, no irritation, no compatibility contraindication with prescription drugs, safety in use, easy film forming of the medicine, short film forming time and no easy falling off.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pharmaceutical preparations, and particularly relates to a traditional Chinese medicine composition for promoting wound healing, a film coating agent and a preparation method and application thereof. BACKGROUND

[0002] The meat of the castrated cock is tender, and the cock is more docile and easier to fatten, so the economic value is higher. The castrated cock on the market is usually castrated by removing the testicles at the age of 25-30 days. However, the wound is usually not sutured after the simple operation, and the wound is often open, so the chick is easy to be infected with bacteria through the wound, and secondary peritonitis and subcutaneous emphysema occur, and then death is caused. In order to prevent this from happening, the current method is to give the chicken oral antibiotics for 3-4 days before castration and 3-4 days after castration to prevent infection, but there is no physical protection, local anti-inflammatory and wound healing effect on the wound, and the chicken with subcutaneous emphysema often has symptoms of respiratory difficulty, causing poor growth.

[0003] The film coating agent refers to a liquid external preparation for dissolving or dispersing drugs in a solvent containing a film-forming material, and forming a thin film after being applied to the affected area. The dosage form has the advantages of targeted drug delivery, accurate therapeutic effect, small amount of drug, long duration of drug effect and the like. However, there are few products and reports about traditional Chinese medicine film coating agents for external use in veterinary medicine. SUMMARY

[0004] The purpose of the present application is to provide a traditional Chinese medicine composition for promoting wound healing, a film coating agent and a preparation method and application thereof. The effective components of the traditional Chinese medicine composition and the film coating agent directly act on the skin wound, and can play the roles of antibacterial, anti-inflammatory, hemostasis, swelling relief and pain relief.

[0005] In order to solve the problems in the prior art, the technical scheme adopted by the present application is as follows:

[0006] In a first aspect, the present application provides a traditional Chinese medicine composition for promoting wound healing, which is prepared from the following raw materials by weight:

[0007] Verbena 3-7 parts, Eleusine indica 1-5 parts, and water nail 0.5-4 parts. Preferably, verbena 5 parts, Eleusine indica 3 parts, and water nail 2 parts.

[0008] In a second aspect, the present application provides a preparation method of the traditional Chinese medicine composition of the first aspect, which comprises the following steps:

[0009] The verbena, Eleusine indica and water nail are dried to constant weight, respectively, and then mixed uniformly after being crushed.

[0010] In a third aspect, the present application provides a preparation method of a traditional Chinese medicine film coating agent for promoting wound healing, which comprises the following steps:

[0011] (1) Preparation of flow extract: the traditional Chinese medicine composition prepared in the second aspect is soaked with ethanol for 24-36 h, extracted by water bath reflux for 1-3 h, and then filtered to remove the residue. The filtrate is fully recovered with ethanol at 50-55°C to obtain a flow extract;

[0012] (2) Preparation of matrix:

[0013] The PVA-1788 is soaked with ultrapure water and heated in a water bath at 90°C or above with stirring until completely dissolved; the PVP K-30 is added to ultrapure water and heated in a water bath at 50-55°C with stirring until completely dissolved; the PVA-1788 aqueous solution at 45-55°C is added with the PVP K-30 aqueous solution at 50-55°C, and stirred uniformly on a magnetic stirrer to obtain the matrix;

[0014] (3) The excipients glycerol, Tween-80 and defoaming agent are sequentially added to the matrix, and stirred uniformly on a magnetic stirrer at 45-55°C to obtain a drug carrier;

[0015] (4) The flow extract prepared in step (1) is dissolved with anhydrous ethanol to obtain a drug-containing anhydrous ethanol, which is added to the drug carrier prepared in step (3) and continuously stirred for 10-20 min during natural cooling to uniformly mix the components, thereby obtaining a traditional Chinese medicine film-coating agent. The film-coating agent is cooled to room temperature and sealed for storage at room temperature. In this step, the flow extract is first dissolved with anhydrous ethanol, which is beneficial to obtaining a more complete film-coating agent.

[0016] Further, the solid-liquid ratio of the mixture of Verbena officinalis, Eupatorium adenophorum and water to ethanol in step (1) is 100 g: 1000-2000 ml.

[0017] Further, the PVA-1788 in step (2) is soaked with 7-9 times the weight of ultrapure water for 24-36 h to fully swell in water; the PVP K-30 is added to 7-9 times the weight of ultrapure water.

[0018] Further, the final concentration of the flow extract in the traditional Chinese medicine film-coating agent is 75-125 mg / mL; preferably, the final concentration of the flow extract is 100 mg / mL.

[0019] Further, the mass ratio of the PVA-1788 to the PVP K-30 is 3-6: 1-2.

[0020] Further, the solid-liquid ratio of the PVA-1788 to glycerol, Tween-80, defoaming agent, anhydrous ethanol is 3-6 g: 2.75-5.5 mL: 0.2-0.4 mL: 0.05-0.1 mL: 4-8 mL.

[0021] Further, the defoaming agent is 2-amino-2-methyl-1-propanol.

[0022] In a fourth aspect, the present application provides a traditional Chinese medicine coating agent for promoting wound healing, which is prepared by the preparation method of the third aspect.

[0023] In a fifth aspect, the present application provides the use of the traditional Chinese medicine coating agent of the fourth aspect in the preparation of a medicine for treating surgical wounds of cockerels.

[0024] The present application has the following advantages and beneficial effects:

[0025] Verbena officinalis L. is used as a medicine, which is cool in nature and slightly bitter in taste, and has the effects of removing blood stasis, dredging channels, clearing heat and detoxifying. The flavonoid components of Verbena officinalis L. can inhibit Staphylococcus aureus, Bacillus subtilis, Escherichia coli and other bacteria, and can also effectively scavenge oxygen free radicals in the body, and has the functions of antifungal, antioxidant and anti-inflammatory. Modern pharmacological studies have shown that Verbena officinalis L. also has the effect of hemostasis. It has also been reported abroad that the extract of Verbena officinalis L. has the effects of antioxidant, antibacterial, anti-inflammatory, neuroprotective, anticancer, analgesic or anticonvulsant.

[0026] Eleusine indica (L.) Gaertn. is a kind of grass of the family Poaceae, and has the functions of clearing heat and dampness, cooling blood and detoxification, and is used externally to treat sore abscess, swelling and pain, and injury from falling and striking.

[0027] Rotala rotundifolia (Buch-Ham. ex Roxb.) Koehne is a kind of grass of the family Lythraceae, and has the functions of clearing heat and dampness, cooling blood and detoxification, and is used externally to treat sore abscess, swelling and pain, and scalding injury.

[0028] The traditional Chinese medicine composition of the present application is prepared by Verbena officinalis L., Eleusine indica (L.) Gaertn. and Rotala rotundifolia (Buch-Ham. ex Roxb.) Koehne in a specific ratio. Verbena officinalis L. has the effects of clearing heat and detoxification, removing blood stasis and antibacterial and anti-inflammatory. Eleusine indica (L.) Gaertn. and Rotala rotundifolia (Buch-Ham. ex Roxb.) Koehne both have the effects of cooling blood and detumescence. The synergistic effect of the three herbs enhances the effects of clearing heat and detoxification, removing blood stasis, antibacterial and anti-inflammatory, so that the present application has the effects of antibacterial and anti-inflammatory, hemostasis, detumescence and analgesia. Through experiments, 75% alcohol extract of the three herbs has a significant bacteriostatic effect on Escherichia coli and Staphylococcus aureus, and the MIC (minimum inhibitory concentration) is 12.5 mg / ml, and the MBC (minimum bactericidal concentration) is 25 mg / ml.

[0029] The prepared coating film agent has good film forming property and flexibility, suitable viscosity, good stability, strong adhesion to skin, no irritation, good biocompatibility, no compatibility contraindication with prescription drugs, safety in use, easy film forming of drugs, short film forming time and no easy falling off.

[0030] The 75% ethanol extract (flow extract) of the traditional Chinese medicine composition in the application is dissolved in anhydrous ethanol, and then added into a coating film agent drug carrier prepared from PVA-1788, PVP K-30, glycerol, Tween-80 and 2-amino-2-methyl-1-propanol, which has multiple effects of antibacterial, anti-inflammatory, hemostatic and analgesic, and can also play a mechanical protective role on skin wounds and has obvious effect of promoting healing of skin wounds, especially the skin wounds of cockerels. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 is the wound healing condition of the sixth day of each test group;

[0032] A is the wound healing condition of the sixth day of the blank group; B is the wound healing condition of the sixth day of the FN group;

[0033] C is the wound healing condition of the sixth day of example 4; D is the wound healing condition of the sixth day of example 5;

[0034] E is the wound healing condition of the sixth day of example 6; F is the wound healing condition of the sixth day of comparative example 3;

[0035] G is the wound healing condition of the sixth day of comparative example 4; H is the wound healing condition of the sixth day of comparative example 5. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the application will be clearly described below with reference to the drawings in the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art belong to the scope of protection of the application.

[0037] The experimental methods in the following examples are all conventional methods, and are carried out according to the techniques or conditions described in the literature in the art or according to the product instructions, unless otherwise specified. The materials, reagents and the like used in the following examples can be obtained from commercial channels, unless otherwise specified.

[0038] Example 1

[0039] The embodiment provides a traditional Chinese medicine composition for promoting wound healing, which is prepared from the following raw medicinal materials in parts by weight:

[0040] Verbena 5 parts, Eleusine indica 3 parts, and water nail 2 parts.

[0041] The preparation method of the traditional Chinese medicine composition comprises the following steps:

[0042] The verbena, the eleusine indica and the water nail are dried at 40 DEG C until constant weight, and then are ultra-micronized and passed through a 100-mesh sieve, and then are uniformly mixed according to the above-mentioned proportion to obtain the traditional Chinese medicine composition powder for promoting wound healing.

[0043] Embodiment 2

[0044] The embodiment provides a traditional Chinese medicine composition for promoting wound healing, which is prepared from the following raw medicinal materials in parts by weight:

[0045] Verbena 7 parts, Eleusine indica 1 part, and water nail 1 part.

[0046] Embodiment 3

[0047] The embodiment provides a traditional Chinese medicine composition for promoting wound healing, which is prepared from the following raw medicinal materials in parts by weight:

[0048] Verbena 3 parts, Eleusine indica 5 parts, and water nail 4 parts.

[0049] Embodiment 4

[0050] The embodiment provides a preparation method of a traditional Chinese medicine coating agent for promoting wound healing, which comprises the following steps:

[0051] (1) preparing a flow extract: 100 g of the traditional Chinese medicine composition powder prepared in Embodiment 1 is soaked in 1000 ml of 75% ethanol in volume concentration for 24 h, and is extracted by water bath reflux for 3 h, and then the residue is filtered, and the filtrate is fully recovered of ethanol by a rotary evaporator at 55 DEG C to obtain a flow extract.

[0052] (2) Preparation of the matrix: first weigh 3 g of PVA-1788, soak in 24 mL of ultrapure water for 24 h, let it swell in water, heat in water bath above 90°C, stir constantly while heating, until completely dissolved, the solution becomes a gel without particles and transparent. Add 1 g of PVP K-30 to 8 mL of ultrapure water, heat in water bath at 50-55°C and stir, stir constantly while heating, until completely dissolved, the solution becomes a gel without particles and transparent. When the PVA-1788 aqueous solution is about 50°C, add the 50°C PVP K-30 aqueous solution, stir evenly on a magnetic stirrer at 200 rpm, and obtain a viscous matrix;

[0053] (3) Add the following excipients to the viscous matrix in turn: glycerol 2.75 mL, 0.2 mL Tween-80, 0.05 mL 2-amino-2-methyl-1-propanol, stir evenly on a magnetic stirrer at 50°C and 200 rpm, and obtain a viscous drug carrier;

[0054] (4) Dissolve the flow extract prepared in step (1) with 4 mL of absolute ethanol to obtain a drug-containing absolute ethanol, and add the drug-containing absolute ethanol to the viscous drug carrier prepared in step (3) to make the final concentration of flow extract in the traditional Chinese medicine film coating agent 100 mg / mL. Continue stirring for 10-20 min during natural cooling to mix the ingredients evenly, and obtain a viscous traditional Chinese medicine film coating agent. After cooling to room temperature, seal and store at room temperature in the dark.

[0055] Example 5

[0056] This example provides a traditional Chinese medicine film coating agent for promoting wound healing. The difference between this example and Example 4 is only that the final concentration of flow extract in the traditional Chinese medicine composition film coating agent is 75 mg / mL. The rest is the same as Example 4.

[0057] Example 6

[0058] This example provides a traditional Chinese medicine film coating agent for promoting wound healing. The difference between this example and Example 4 is only that the final concentration of flow extract in the traditional Chinese medicine composition film coating agent is 125 mg / mL. The rest is the same as Example 4.

[0059] Comparative Example 1

[0060] This comparative example provides a traditional Chinese medicine composition for promoting wound healing. The difference between this comparative example and Example 1 is only that the raw materials for preparing the traditional Chinese medicine composition are different.

[0061] The traditional Chinese medicine composition in this comparative example is prepared from the following raw materials in parts by weight:

[0062] Verbena 5 parts, Eleusine indica 3 parts, Paris polyphylla 2 parts. The rest is the same as Example 1.

[0063] Comparative Example 2

[0064] The present comparative example provides a traditional Chinese medicine composition for promoting wound healing. The difference between the present comparative example and Example 1 is only that the ratio of the raw materials for preparing the traditional Chinese medicine composition is different.

[0065] The traditional Chinese medicine composition in the present comparative example is prepared from the following raw materials in parts by weight:

[0066] Verbena 12 parts, Chinese alpine rush 3 parts, water fingernail 2 parts. The rest is the same as Example 1.

[0067] Comparative Example 3

[0068] The present comparative example provides a preparation method of a traditional Chinese medicine film coating agent for promoting wound healing. The difference between the present comparative example and Example 4 is only that the amount of anhydrous ethanol added in step (4) is different. In the present comparative example, the amount of anhydrous ethanol added in step (4) is 15 mL. The rest is the same as Example 4.

[0069] Comparative Example 4

[0070] The present comparative example provides a preparation method of a traditional Chinese medicine film coating agent for promoting wound healing. The difference between the present comparative example and Example 4 is only that the final concentration of the flow extract in the traditional Chinese medicine composition film coating agent is 150 mg / mL. The rest is the same as Example 4.

[0071] Comparative Example 5

[0072] The present comparative example provides a preparation method of a traditional Chinese medicine film coating agent for promoting wound healing. The difference between the present comparative example and Example 4 is only that in step (2), the PVP K-30 aqueous solution at room temperature is added to the PVA-1788 aqueous solution which has reached 30°C, and stirred uniformly on a magnetic stirrer at 200 rpm. The rest is the same as Example 4.

[0073] Experimental Example 1

[0074] 1 Test materials

[0075] 1.1 Reagents and drugs

[0076] Polyvinyl alcohol 1788 (PVA-1788), polyvinylpyrrolidone K30 (PVP K-30), 2-amino-2-methyl-1-propanol, chemical pure) were purchased from Sigma Aldrich (Shanghai) Trading Co., Ltd.;

[0077] Polysorbate 80 (Tween-80, chemical pure), anhydrous ethanol, glycerol were purchased from Xilong Scientific Chemicals;

[0078] Ultrapure water was provided by the Department of Chemistry, Xichang University.

[0079] 1.2 Test instruments

[0080] Rotary evaporator, vacuum pump, pipette, glass rod, beaker, electric heating jacket, electronic balance, round bottom flask, condenser, conical flask, rubber bulb dropper, etc. provided by the laboratory of the Department of Chemistry, Sichuan University of Arts and Science.

[0081] 2 Method

[0082] 2.1 Preparation of flow extract: The traditional Chinese medicine composition flow extract 35 g was prepared according to the method of Example 4.

[0083] 2.2 Preparation of film coating agent matrix and drug carrier:

[0084] PVA-124, PVA-1788, and PVP K-30 were selected as the main film-forming materials. An appropriate amount of PVA-124 and PVA-1788 was placed in different beakers and soaked in 8 times the weight of ultrapure water for 24 h in advance. After the materials were fully swollen in water, they were heated and stirred in a water bath above 90°C until completely dissolved, and then stored for later use. An appropriate amount of PVP K-30 was placed in a beaker, 8 times the weight of ultrapure water was added, and then heated and stirred in a water bath above 50°C until completely dissolved to obtain the film coating agent matrix.

[0085] After adding glycerol, Tween-80, anhydrous ethanol, and 0.05 ml of defoaming agent as auxiliary materials to the above film materials and stirring uniformly, a viscous film coating agent drug carrier was obtained. The specific amount and compatibility were selected according to 2.4.

[0086] 2.3 Selection of film-forming materials for film coating agent and detection of film-forming effect

[0087] According to the composition and ratio of the film-forming materials in Table 1, the film coating agent drug carrier was prepared by the method of 2.2, and its film-forming effect was detected according to the film coating agent drug carrier scoring standard (Table 2).

[0088] The drug carrier was evenly coated on the surface of a 2.5 x 4 cm skin. Under the condition that other factors remained unchanged, the film-forming solution ratio was tested. The film-forming properties a, uniformity b, bubble amount c, and whether it was easy to peel off d were used as evaluation indexes to evaluate the drug carrier. The scores of each index were multiplied by a certain weight and then added together to obtain the score Y.

[0089] Since the drug carrier needs to be applied to the surface of the chicken's wound, in order to reduce the pecking or wiping off of the film coating agent by the chicken, the weight of film-forming time and film-forming property was set to 0.3, and the weights of uniformity b, bubble amount c, and whether it was easy to peel off d were set to 0.2, 0.2, and 0.1, respectively.

[0090] The score is calculated according to the following formula: Y = (a + e) x 0.3 + (b + c) x 0.2 + d x 0.1, the higher the score, the better the film forming effect.

[0091] Table 1 Different film forming matrix ratio

[0092]

[0093] Table 2 Coating agent drug carrier scoring details

[0094]

[0095] 2.4 Optimization of coating agent excipient ratio

[0096] According to the results of 2.3, the film forming matrix (A) with the best film forming effect was determined, and the amount and ratio of its excipients glycerol (B), Tween-80 (C) and anhydrous ethanol (D) were further explored.

[0097] After fixing A (film material mass + 8 times mass of ultrapure water preparation) and defoaming agent 50 μL, the excipients were added according to Table 3, and the prepared coating agent was comprehensively evaluated according to the evaluation method of Table 2 and the elongation at break (f), and the comprehensive score Y' = Y + f. The test method of elongation at break is to stretch the film agent at a constant speed in the horizontal direction, and the ratio of the size at break to the original size (1 cm) is the elongation at break. Repeat three times and take the average value as the final elongation at break (f).

[0098]

[0099] Table 3 Coating agent excipient ratio

[0100]

[0101] 2.5 Pharmacodynamic test

[0102] Select 130 healthy male chicks of 882 yellow chickens aged 30 days and weighing between 200-250g, the male chicks are purchased from Liangshan State Wu's Farming and Breeding Development Co., Ltd., the feathers on the right side of the last rib of each male chick are plucked, the skin is disinfected, and a 2-3 cm long skin incision is made on the rib with a surgical knife, the skin incision is moved 1 cm to the left and cut through the abdominal muscle down to the peritoneum, and then randomly divided into 13 groups, 10 chickens in each group. Divided into traditional Chinese medicine composition examples 1-3 groups; traditional Chinese medicine coating agent (FM) includes low dose group prepared in example 5, medium dose group prepared in example 4, and high dose group prepared in example 6; coating agent group without drug (FN group); blank group without drug; traditional Chinese medicine composition comparative examples 1-2 groups; traditional Chinese medicine coating agent comparative examples 3-5 groups.

[0103] The use method of each group is as follows:

[0104] The Chinese medicine composition examples 1-3 groups and Chinese medicine composition comparative examples 1-2 groups: after the skin wound is disinfected with alcohol, the skin wound and the skin around the skin wound about 1 cm are directly and evenly smeared, the usage amount is 1-3 ml, and only one time is used.

[0105] The wound sites of the chickens in the FN group, the example 4-6 groups, and the comparative example 3-5 groups are respectively smeared with the corresponding 1-3 ml of the film coating agent, and after one time of smearing, the wound healing conditions, the mental states, the wound inflammation conditions, and the survival rates of the chickens in each group are observed and recorded on the 2nd, 4th, and 6th days. The evaluation of the wounds is evaluated as follows:

[0106] On the 6th day after smearing, if the wound is not swollen, flat and closed, scabbed, the wound covering film is complete and dry, there is no exudate, no emphysema, and the mortality rate is less than or equal to 10%, it is excellent; if each layer of the wound is everted and swollen, not fully closed, there is a small amount of bleeding or exudate, a small amount of emphysema, and the mortality rate is more than 10% but less than or equal to 30%, it is good; if the wound is everted and red and swollen or black and necrotic, not closed, there is a large amount of blood or pus exudate, a large area of emphysema, and breathing difficulty, and the mortality rate is more than 30%, it is poor.

[0107] 2.6 Detection of film forming effect of the Chinese medicine film coating agent

[0108] The Chinese medicine film coating agent with the best effect obtained in 2.5 is detected for the film forming effect according to the method of 2.4, repeated for 3 times, and the film forming time is recorded.

[0109] 3 Results

[0110] 3.1 Optimization results of the film forming material ratio in the film forming base

[0111] It can be seen from Table 4 that PVA-124 and PVP-K30 cannot be used alone, PVA-1788 has strong viscosity and is not easy to remove, and bubbles are easily generated during dissolution; PVP K-30 forms a film quickly, but the film forming property is poor; the comprehensive score of PVA-124 used alone is also low. The comprehensive scores of PVA-124:PVP K-30=3:1 and PVA-124:PVP K-30=4:1 are the same. In order to save materials, the former is used to continue to explore the addition amount of other adjuvants (glycerol, Tween-80, anhydrous ethanol) and the addition amount of the extract of the Chinese medicine composition.

[0112] Table 4 Influence of different film forming material ratios on the film coating agent score

[0113]

[0114]

[0115] 3.2 Optimization results of the adjuvant addition amount of the film coating agent

[0116] The results show that when the film-forming material is PVA-1788 8g and PVP K-30 1g, the optimal content of glycerol is 2.75mL, the optimal content of Tween-80 is 0.2mL, and the optimal content of anhydrous ethanol is 4mL (the combination number corresponds to Table 3). The specific results are shown in Table 5.

[0117] Table 5 Optimization results of coating agent adjuvants

[0118]

[0119] 3.3 Effects of the traditional Chinese medicine composition and the coating agent of the present application on the healing of surgical wounds of male chicks

[0120] The results show that, in addition to the death of male chicks in the FM groups on the 6th day of using the coating agent, the mortality rates of the blank group and the FN group are 100%; the healing of the wounds in the FM groups is significantly better than that in the other groups, and the FM medium-dose group has the best effect.

[0121] The test results are shown in Table 6 and Figure 1 On the 6th day after application, the wounds of each layer are not swollen, flat and closed, scabbed, the covering film of the wounds is complete and dry, there is no exudate, no emphysema, and the mortality rate is ≤10%, which is excellent; the wounds of each layer are swollen and everted, not fully closed, with a small amount of bleeding or exudate, a small amount of emphysema, and the mortality rate is 10%<30%, which is good; the wounds are everted, red and swollen, or black and necrotic, not closed, with a large amount of blood or pus exudate, a large area of emphysema, and difficulty breathing, and the mortality rate is >30%, which is poor.

[0122] Table 6 Comparison of application effects

[0123]

[0124]

[0125]

[0126] As shown in Table 6, the traditional Chinese medicine composition prepared in Examples 1-3 has antibacterial, anti-inflammatory and detumescence effects on the external wounds of male chicks, and the traditional Chinese medicine composition prepared in Example 1 has a better effect than that prepared in Examples 2 and 3. Examples 4-6 are ethanol paste of ultra-fine powder of the traditional Chinese medicine composition prepared into a coating agent on the basis of Example 1. The coating agent prepared by the preparation method of Examples 4-6 not only has obvious antibacterial and anti-inflammatory effects on the external wounds of male chicks, but also covers the wounds to a certain extent, plays a mechanical protective role, and makes the healing of the wounds more obvious, especially the traditional Chinese medicine coating agent prepared in Example 4 has the best effect.

[0127] 3.4 The film-forming effect detection results of the traditional Chinese medicine composition coating agent prepared in Examples 4-6 and Comparative Examples 3-5 are shown in Table 8.

[0128] Y = (a + e) x 0.3 + (b + c) x 0.2 + d x 0.1

[0129] Table 7 Coating agent score details

[0130]

[0131] The comprehensive score Y' = Y + f. The test method of elongation at break (f) is to uniformly stretch the film agent in the horizontal direction, and the ratio of the size at break to the original size (1 cm) is the elongation at break.

[0132] Table 8 Coating agent film forming effect test

[0133]

[0134] 4Discussion

[0135] A large number of pre-tests were conducted before the experiment, and PVA-2699 and PVA-2488 were selected as film forming materials to be matched with starch for testing, but the film forming time was too long, and almost all of them needed more than 20 minutes to form a film. Subsequently, the addition of volatile agent anhydrous ethanol reduced the film forming time, but the film forming time was still about 15 minutes, so this two types of film forming materials were abandoned. PVA-124, PVA-1788 and PVP K-30 were selected as film forming materials for screening. In the screening process, the inventors found that PVA-124 and PVA-1788 were soaked in ultrapure water or distilled water for 24 hours in advance to allow them to fully absorb water and swell. In the pre-test stage, the ratio of PVA to ultrapure water was set to 1:5, and the results showed that this ratio could not allow PVA to fully absorb water and swell. Then the ratio of PVA to ultrapure water was set to 1:16, and the results showed that too much water caused the solution to be insufficiently viscous after the PVA was heated and melted, and the film forming time was prolonged due to the addition of too much ultrapure water. Then the ratio of PVA to ultrapure water was adjusted to 1:8, and at this time the PVA could fully absorb water and swell. After heating and melting, the solution had just the right viscosity, i.e. it had flowability and was sticky when touched by hand.

[0136] As can be seen from Table 6, the higher the final concentration of the decoction pieces, the better the treatment effect of the coating agent of the present application. The reason for this result may be that the in vitro penetration rate of the decoction pieces has a saturation value, and the higher the concentration, the more negative effect on film forming.

[0137] As can be seen from Table 8, the coating film agent prepared in Examples 4-6 is significantly superior to Comparative Examples 3-5 in film-forming property, uniformity, ease of peeling, film-forming time, elongation at break, etc. Among them, the coating film agent prepared in Example 4 has the best performance. Comparative Examples 3 and 5 have the worst film-forming property. The coating film agent prepared in Comparative Example 4 is superior to Comparative Examples 3 and 5, but still has a large gap compared with Examples 4-6. The coating film agent prepared in Comparative Example 5 is prepared by adding the PVP K-30 aqueous solution at room temperature into the PVA-1788 aqueous solution at 30°C. Since the two materials are not fully dissolved in water at a low temperature, the two film materials cannot be fully mixed, and the uniformity and film-forming property are poor.

[0138] The embodiments of the present application are described above with reference to the drawings; however, the present application is not limited to the specific embodiments described above, but the specific embodiments described above are merely illustrative and not restrictive, and a person of ordinary skill in the art can make many forms under the inspiration of the present application without departing from the scope of the present application and the scope protected by the claims.

Claims

1. A Chinese medicine composition for promoting wound healing, characterized in that: Prepared from the following raw materials in parts by weight: 3-7 parts of verbena, 1-5 parts of goosegrass, and 0.5-4 parts of water nail.

2. The method for preparing a Chinese medicine composition for promoting wound healing according to claim 1, wherein: The following steps are involved: The verbena, goosegrass and water nails in the aforementioned weight portions are dried to constant weight, crushed and then mixed evenly.

3. A method for preparing a traditional Chinese medicine film-coating agent for promoting wound healing, characterized in that: The following steps are involved: (1) Preparation of fluid extract: Soak the Chinese medicine composition prepared in claim 2 in ethanol for 24-36 hours, extract under reflux in a water bath for 1-3 hours, filter the residue, and fully recover ethanol from the filtrate at 50-55°C to obtain a fluid extract; (2) Preparation of matrix: Soak PVA-1788 in ultrapure water, heat and stir in a water bath above 90°C until completely dissolved and set aside; add PVP K-30 to ultrapure water, heat and stir in a water bath at 50-55°C until completely dissolved and set aside; add a 50-55°C PVP K-30 aqueous solution to a 45-55°C PVA-1788 aqueous solution, and stir evenly on a magnetic stirrer to obtain the matrix; (3) Add the excipients glycerol, Tween-80, and defoaming agent to the matrix in sequence, stir evenly on a magnetic stirrer at 45-55°C to obtain a drug carrier; (4) The fluid extract prepared in step (1) is dissolved in anhydrous ethanol to obtain drug-containing anhydrous ethanol, and the drug-containing anhydrous ethanol is added to the drug carrier prepared in step (3). The mixture is stirred for 10-20 minutes during natural cooling to uniformly mix the components to obtain a Chinese medicine film-coating agent. The film-coating agent is cooled to room temperature and then sealed and stored at room temperature.

4. The method for preparing a Chinese medicine film-coating agent for promoting wound healing according to claim 3, wherein: In the step (1), the solid-liquid ratio of the mixture obtained by uniformly mixing verbena, goosegrass and water nail to ethanol is 100g:1000ml-2000ml.

5. The method for preparing a traditional Chinese medicine film-coating agent for promoting wound healing according to claim 3, wherein: In step (2), the PVA-1788 is soaked in 7-9 times its weight of ultrapure water for 24-36 hours to allow it to fully swell in the water; the PVP K-30 is added in 7-9 times its weight of ultrapure water.

6. The method for preparing a traditional Chinese medicine film-coating agent for promoting wound healing according to claim 3, wherein: The final concentration of the fluid extract in the traditional Chinese medicine film-coating is 75-125 mg / mL.

7. The method for preparing a traditional Chinese medicine film-coating agent for promoting wound healing according to claim 3, wherein: The mass ratio of the PVA-1788 to the PVP K-30 is 3-6:1-2.

8. The method for preparing a traditional Chinese medicine film-coating agent for promoting wound healing according to claim 3, wherein: The solid-liquid ratio of the PVA-1788 to glycerol, Tween-80, defoaming agent, and anhydrous ethanol is 3-6 g: 2.75-5.5 mL: 0.2-0.4 mL: 0.05-0.1 ml: 4-8 mL.

9. A Chinese medicine film-coating agent for promoting wound healing, characterized in that The invention is prepared by the preparation method according to any one of claims 3 to 8.

10. Use of the traditional Chinese medicine film-coating agent according to claim 9 in preparing medicine for treating surgical wounds in male chickens.

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