A device and method for cleaning a culture bag and substrate of a tissue culture seedling without damaging the seedling

Abstract

The application discloses a device and method for tearing a culture bag and cleaning a medium for aseptic in-vitro seedling, wherein the device for tearing the culture bag and cleaning the medium for the aseptic in-vitro seedling comprises a culture bag tearing device and an in-vitro seedling cleaning device, the culture bag tearing device is used for tearing the culture bag, and the in-vitro seedling cleaning device is used for cleaning the in-vitro seedling; after the culture bag is torn, the in-vitro seedling in the culture bag falls into the in-vitro seedling cleaning device. The technical scheme of the application can replace manual work to tear the plastic culture bag and clean the in-vitro seedling, only needs manual light participation in assistance in the working process, is high in work efficiency, low in labor cost, does not damage the in-vitro seedling, effectively reduces customer loss, and is good in practicability.

Description

Technical Field

[0001] This invention relates to the field of tissue culture seedlings, and in particular to an apparatus and method for removing tissue culture seedling culture bags and cleaning the substrate without causing damage. Background Technology

[0002] As is well known, tissue culture seedlings are sold in large quantities after they have matured. In order to facilitate transportation and ensure the safety of the seedlings during transportation and to prevent them from dying, they are usually simply packaged in plastic bags, sealed, and labeled with seedling information before being packed and transported.

[0003] Each plastic culture bag contains multiple tissue culture seedlings. Currently, after receiving the goods, customers manually tear open the plastic culture bag, remove the tissue culture seedlings, and clean them. After washing away the substrate on the seedlings, they plant them in the ground. The entire process requires a high degree of manual intervention, incurring significant labor costs for customers. Moreover, manual operation is inefficient. In addition, manually tearing open the plastic culture bag and cleaning the seedlings can easily damage them, causing considerable harm to customers. Summary of the Invention

[0004] The main objective of this invention is to provide a device and method for removing tissue culture bags and cleaning the substrate without causing damage, in order to solve the problems of low efficiency, high labor costs, and easy damage to tissue culture seedlings caused by the current practice of manually tearing open plastic culture bags and cleaning tissue culture seedlings.

[0005] To address the aforementioned problems, this invention proposes a device for non-invasively removing tissue culture seedling culture bags and cleaning the substrate, comprising a culture bag tearing device and a tissue culture seedling cleaning device. The culture bag tearing device is used to tear open the culture bag, and the tissue culture seedling cleaning device is used to clean the tissue culture seedlings. After the culture bag is torn open, the tissue culture seedlings inside the culture bag fall into the tissue culture seedling cleaning device.

[0006] The culture bag tearing device includes:

[0007] A chassis on which a tube is vertically mounted, and a motor connected to the tube is installed inside the chassis, which drives the tube to rotate.

[0008] A turntable is coaxially fixed to the upper end of the tube body. Multiple suction nozzles are evenly distributed around the lower surface of the turntable. The suction nozzles are connected to an air pump through a pipe. A control valve is installed on the pipe.

[0009] The tearing mechanism is located below the suction nozzle and is fixedly connected to the machine casing. The tearing mechanism has a cylinder and a pair of grippers on the cylinder. The pair of grippers move closer or further apart under the drive of the cylinder. The grippers can clamp a corner of the culture bag.

[0010] The tissue culture seedling cleaning device includes multiple cleaning boxes arranged in a straight line, with adjacent cleaning boxes connected together, and the multiple cleaning boxes can move along their arrangement direction.

[0011] Each cleaning tank is equipped with a partition 2, which divides the cleaning tank into a liquid storage area and a cleaning area. A drain pipe is installed at the bottom of the cleaning area, and a control valve 2 is installed on the drain pipe.

[0012] The cleaning zone is equipped with an elastic mesh plate that protrudes upwards in the middle. The elastic mesh plate is located below the water surface in the cleaning zone, and its projection on the bottom of the cleaning zone covers the bottom of the cleaning zone. Connecting plates are fixedly installed at opposite ends of the elastic mesh plate. The upper end of the connecting plate extends out of the cleaning tank and is hinged to the cleaning tank. At the same time, a lifting device is also connected to the upper end of the connecting plate. The lifting device is fixedly connected to the cleaning tank. The lifting device drives the connecting plate to rotate around its hinge position with the cleaning tank, thereby moving the elastic mesh plate up and down and changing the degree of protrusion of the elastic mesh plate.

[0013] Several flushing pipes are installed above and below the elastic mesh plate in the cleaning zone. Multiple nozzles are installed on the flushing pipes at intervals. The multiple nozzles on the multiple flushing pipes can spray and flush all areas of the upper and lower surfaces of the elastic mesh plate and the connecting plate located in the cleaning zone. The flushing pipes are sealed and penetrate the partition plate to extend into the liquid storage area and are connected to the water pump in the liquid storage area.

[0014] In one embodiment, a cover is detachably installed on the upper end of the chassis, a partition is provided inside the chassis, the lower end of the tube passes through the cover and the partition and is rotatably connected to the cover and the partition, and a retaining ring is provided on the lower surface of the cover and the upper surface of the partition. The retaining ring is coaxially fixedly connected to the tube and prevents the tube from moving axially.

[0015] A motor is installed below the partition plate inside the chassis. The motor is connected to a gear ring via gear transmission. The gear ring is coaxially and fixedly connected to the lower end of the tube body.

[0016] In one embodiment, the air pump is fixedly connected to the turntable, the pipeline includes an air intake pipe, an annular pipe and a connecting pipe, the air intake end of the air pump is connected to the annular pipe through the air intake pipe, the suction nozzle is connected to the annular pipe through the connecting pipe, and a control valve is installed on each connecting pipe.

[0017] In one embodiment, the culture bag tearing device further includes a bag holding box, which is located below the suction nozzle.

[0018] In one embodiment, the connecting plate is inverted L-shaped, with its lower end attached to the inner wall of the cleaning tank.

[0019] In one embodiment, the bottom of the cleaning tank is provided with wheels, and a track is provided below the wheels, so that the wheels roll along the track.

[0020] A drainage ditch is provided directly below the drainage pipe, and the drainage ditch extends along the length of the track.

[0021] In one embodiment, the tissue culture seedling cleaning device further includes a water inlet pipe located above the cleaning tank. The water inlet pipe is equipped with two control valves, which respectively control the water inlet pipe to add water to the storage area and the cleaning area.

[0022] Furthermore, this aspect also proposes a method for removing tissue culture seedling culture bags and cleaning the substrate without causing damage, wherein the following steps are performed using any of the aforementioned devices for removing tissue culture seedling culture bags and cleaning the substrate without causing damage:

[0023] S1. Control the motor to drive the turntable to rotate, so that the suction nozzle moves to the suction bag position;

[0024] S2. Add water to the liquid storage area and cleaning area of ​​the cleaning tank;

[0025] S3. Move the cleaning box directly below the suction nozzle in the suction bag position;

[0026] S4. Contact the culture bag with the suction nozzle in the suction position, and use the suction nozzle to hold and lift the culture bag.

[0027] S5. Control the cylinder to drive a pair of grippers closer together, then control the pair of grippers to clamp the lower ends of the culture bag respectively. Then control the cylinder to drive the pair of grippers away from each other to tear open the lower part of the culture bag. The tissue culture seedlings in the culture bag fall into the water in the cleaning area, while the upper part of the culture bag is kept sucked by the suction nozzle.

[0028] S6. Control the pair of grippers to release the clamping of the culture bag, and then repeat S1-S5 to move the next nozzle to the suction bag position and move the next cleaning box directly below the nozzle in the suction bag position.

[0029] S7. The tissue culture seedlings float in the water. Control the lifting device to move the elastic mesh plate up and increase the degree of protrusion of the elastic mesh plate so that the elastic mesh plate lifts the tissue culture seedlings and separates them from the water surface. Then control the elastic mesh plate to descend and reset, and the tissue culture seedlings fall back into the water. By controlling the elastic mesh plate to move up and down multiple times, the water in the cleaning area continuously and protectively beats the tissue culture seedlings until the multiple tissue culture seedlings that have gathered together are dispersed.

[0030] S8. Drain the water in the cleaning area and control the nozzles to spray water onto the tissue culture seedlings scattered on the elastic mesh plate to rinse the tissue culture seedlings a second time and wash away the substrate on the tissue culture seedlings.

[0031] S9. When the empty culture bag below the suction nozzle moves to the top of the bag holding box, control the suction nozzle to release the suction of the culture bag, and the culture bag falls into the bag holding box.

[0032] In one embodiment, the linkage control of S1-S3 and S9 is performed synchronously.

[0033] Beneficial effects: The technical solution of this application can replace manual labor in tearing open plastic culture bags and cleaning tissue culture seedlings. Only light manual assistance is required during the work process. It has high work efficiency, low labor costs, and will not damage the tissue culture seedlings, effectively reducing customer losses and has good practicality. Attached Figure Description

[0034] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0035] Figure 1 This is a top view of a device for removing tissue culture seedling culture bags and cleaning substrate without causing damage according to the present invention;

[0036] Figure 2 yes Figure 1 AA section view in the middle;

[0037] Figure 3 This is a schematic diagram showing the spray direction and angle range of the nozzle of the tissue culture seedling cleaning device of the present invention;

[0038] Figure 4 This is a top view of the cleaning box of the present invention after removing the connecting plate and the elastic mesh plate;

[0039] Figure 5 This is a schematic diagram of the tearing action of the culture bag of the present invention.

[0040] The annotations in the attached figures are explained as follows:

[0041] 1. Chassis; 2. Cover; 3. Partition 1; 4. Retaining ring; 5. Pipe body; 6. Wire; 7. Gear ring; 8. Gear; 9. Motor; 10. Base plate; 11. Turntable; 12. Suction nozzle; 13. Control valve 1; 14. Connecting pipe; 15. Circular pipe; 16. Machine cover; 17. Suction pipe; 18. Culture bag; 19. Bag holding box; 20. Cleaning box; 21. Partition 2; 22. Liquid storage area; 23. Cleaning area; 24. Drain pipe 25. Control Valve II; 26. Drainage Ditch; 27. Flushing Pipe; 28. Sprayer Nozzle; 29. ​​Connecting Pipe; 30. Water Pump; 31. Lifting Device; 32. Connecting Plate; 33. Elastic Mesh Plate; 34. Water Surface; 35. Water Inlet Pipe; 36. Control Valve III; 37. Wheel; 38. Track; 39. Foot Switch; 40. Connector; 41. Ground; 42. Sealing Line; 43. Rodless Cylinder; 44. Electric Gripper; 45. Mounting Rod. Detailed Implementation

[0042] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0043] It should be noted that if the embodiments of the present invention involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.

[0044] In this invention, unless otherwise explicitly specified and limited, the terms "connection," "fixed," etc., should be interpreted broadly. For example, "fixed" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0045] Furthermore, if the embodiments of this invention involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this invention.

[0046] This invention proposes a device for removing tissue culture bags 18 and cleaning the substrate without causing damage. This device can replace manual labor in tearing open plastic culture bags 18 and cleaning tissue culture seedlings. Only minor manual assistance is required during the operation. It has high efficiency, low labor costs, and does not damage the tissue culture seedlings, effectively reducing customer losses and has good practicality.

[0047] Specifically, the device for removing the tissue culture seedling culture bag 18 and cleaning the substrate without damage includes a culture bag 18 tearing device and a tissue culture seedling cleaning device. The culture bag 18 tearing device is used to tear open the culture bag 18, and the tissue culture seedling cleaning device is used to clean the tissue culture seedlings. After the culture bag 18 is torn open, the tissue culture seedlings inside the culture bag 18 fall into the tissue culture seedling cleaning device. Therefore, the culture bag 18 tearing device and the tissue culture seedling cleaning device work together to complete the preparation work before planting the tissue culture seedlings.

[0048] Specifically, such as Figure 1 and Figure 2 As shown, the culture bag 18 tearing device includes: a housing 1, a turntable 11, and a tearing mechanism. A tube 5 is vertically mounted on the housing 1. A cover 2 is detachably installed on the upper end of the housing 1. A partition 3 is provided inside the housing 1. The lower end of the tube 5 passes through the cover 2 and the partition 3 and is rotatably connected to the cover 2 and the partition 3. A retaining ring 4 is provided on the lower surface of the cover 2 and the upper surface of the partition 3. The retaining ring 4 is coaxially fixedly connected to the tube 5 to prevent the tube 5 from moving axially. A motor 9 is provided below the partition 3 inside the housing 1. The motor 9 is driven by a gear 8 and a gear ring 7. The gear ring 7 is coaxially fixedly connected to the lower end of the tube 5. With this design, the motor 9 can drive the tube 5 to rotate.

[0049] In this embodiment, as Figure 1 and Figure 2As shown, the turntable 11 is coaxially fixed to the upper end of the tube body 5. Multiple suction nozzles 12 are evenly distributed circumferentially on the lower surface of the turntable 11. The suction nozzles 12 are connected to a vacuum pump via pipes, and a control valve 13 is installed on the pipes. Specifically, the vacuum pump is fixedly connected to the turntable 11, and a cover 16 is provided to protect the vacuum pump. The pipes include a suction pipe 17, an annular pipe 15, and a connecting pipe 14. Figure 1 and Figure 2 As shown, the suction end of the air pump is connected to the annular pipe 15 through the suction pipe 17, and the suction nozzle 12 is connected to the annular pipe 15 through the connecting pipe 14. Each connecting pipe 14 is equipped with a control valve 13. After the air pump is started, the opening and closing of the control valve 13 is controlled to control whether the corresponding suction nozzle 12 has the ability to adsorb the culture bag 18.

[0050] In this embodiment, as Figure 1 and Figure 2 As shown, the tearing mechanism is located below the suction nozzle 12 and is fixedly connected to the housing 1 via the mounting rod 45. The tearing mechanism has a cylinder and a pair of grippers mounted on the cylinder. The pair of grippers move closer or further apart under the action of the cylinder, and the grippers can clamp a corner of the culture bag 18; specifically, as shown... Figure 2 and Figure 5 As shown, there is a portion of the bag body below the sealing line 42 of the culture bag 18. No tissue culture seedlings are embedded in this portion, so clamping this area with the grippers will not damage the seedlings. Figure 5 The dotted circle shown is the clamping area of ​​the grippers. After a pair of grippers clamp this area at both ends of the lower part of the culture bag 18, they move away from each other under the action of the cylinder, which can tear the lower part of the culture bag 18 apart.

[0051] Preferably, the cylinder is a rodless cylinder 43 and the gripper is an electric gripper 44. This design facilitates the control of the cylinder and the gripper.

[0052] In this embodiment, as Figure 1 and Figure 2 As shown, when the culture bag 18 tearing device is working, it first controls the motor 9 to drive the turntable 11 to rotate, causing the suction nozzle 12 to move to the suction bag position, and then opens the control valve 13 to start the air pump, so that the suction nozzle 12 has suction force. The suction bag position is... Figure 1 The suction nozzle 12 on the turntable 11 is located at the three o'clock position. This suction nozzle 12 is in the suction bag position, such as... Figure 2 As shown, hold the culture bag 18 and bring it close to the suction nozzle 12. The suction nozzle 12 will then hold and lift the culture bag 18. It should be noted that during the process of holding the culture bag 18 and bringing it close to the suction nozzle 12, the position of the culture bag 18 should be adjusted so that the pair of grippers can smoothly clamp the lower two ends of the culture bag 18 when they come close to each other.

[0053] After the suction nozzle 12 sucks up the culture bag 18, the control cylinder drives a pair of grippers to move closer together, and then controls the pair of grippers to clamp the area at both ends of the lower part of the culture bag 18. Then, the control cylinder drives the pair of grippers to move away from each other, tearing open the lower part of the culture bag 18. The tissue culture seedlings inside the culture bag 18 fall into the water in the cleaning area 23, while the upper part of the culture bag 18 remains sucked by the suction nozzle 12.

[0054] After all the tissue culture seedlings in the culture bag 18 have fallen into the water in the cleaning area 23, control a pair of grippers to loosen their clamping on the culture bag 18, and then control the motor 9 to continue to drive the turntable 11 to rotate so that the next suction nozzle 12 moves to the suction bag position.

[0055] When the empty culture bag 18 below the suction nozzle 12 moves directly above the bag holding box 19, the control valve 13 corresponding to the suction nozzle 12 is closed, the suction nozzle 12 releases its suction on the empty culture bag 18, and the culture bag 18 falls into the bag holding box 19.

[0056] In this embodiment, the control motor 9 drives the turntable 11 to rotate, thereby moving the suction nozzle 12 to the suction bag position, which can be controlled by the foot switch 39. Figure 1 The multiple suction nozzles 12 shown are centrally symmetrically distributed. Therefore, by controlling the motor 9 to rotate at a certain angle via the foot switch 39, each suction nozzle 12 can be moved to the suction bag position. Figure 1 For example, the figure shows eight suction nozzles 12 arranged symmetrically at the center. Therefore, by controlling the motor 9 to rotate 45° and then stop by using the foot switch 39, the suction nozzles 12 can be moved to the suction bag position one by one. The rotation direction of the turntable 11 can be as follows: Figure 1 As shown.

[0057] In this embodiment, the air pump can be set to be normally open, starting when people go to work in the morning and turning off when they leave work in the evening, remaining in the normally open state during daytime working hours.

[0058] In this embodiment, the culture bag 18 tearing device also includes a controller and an angle sensor. The angle sensor is used to detect the rotation angle of the tube body 5, and then transmits the detection data to the controller. The controller analyzes and determines the position of each suction nozzle 12, and promptly controls the control valve 13 corresponding to the suction nozzle 12 that has rotated to the suction position to open. This design enables the motor 9 to drive the turntable 11 to rotate and move the suction nozzle 12 to the suction position, at which point the corresponding control valve 13 will automatically open. Conversely, after the motor 9 drives the suction nozzle 12 to move directly above the bag holding box 19, the corresponding control valve 13 will automatically close, and the empty culture bag 18 will automatically fall into the bag holding box 19, which is located on the ground 41 below the suction nozzle 12.

[0059] In this embodiment, after the suction nozzle 12 picks up and lifts the culture bag 18, the action of the control cylinder driving a pair of grippers to move closer together can be manually controlled by another control switch. After the suction nozzle 12 picks up and lifts the culture bag 18, once it is visually determined that the culture bag 18 is in the correct lifting position and there is no risk of it falling, the control switch can be manually operated to control the cylinder to move the pair of grippers closer together. The movement distance and time of the grippers under the action of the cylinder can be designed as fixed values ​​and pre-embedded in the controller to form a control program. Of course, if the specifications and dimensions of the culture bag 18 change, the movement distance and time of the grippers under the action of the cylinder also need to be modified accordingly, and the control program needs to be re-embedded in the controller. With this design, after the manual operation of the control switch controls the cylinder to move, the controller controls the cylinder to move for a certain period of time according to the program. After the cylinder moves for the specified time, it automatically controls the pair of grippers to close and clamp the culture bag according to the program. At the lower ends of the bag 18, the controller automatically controls the cylinder to move a pair of grippers away from each other and reset according to the program, completing the action of tearing open the lower part of the culture bag 18. After the grippers reset for a period of time, the controller automatically controls the pair of grippers to release the clamping of the lower part of the culture bag 18 according to the program. During the period of time that the grippers reset, all the tissue culture seedlings in the culture bag 18 fall into the water in the cleaning area 23. It can be seen that the culture bag 18 tearing device is controlled by the controller according to the control program to automatically complete the work of tearing open the culture bag 18. During the work process, only the human hand needs to bring the culture bag 18 close to the suction nozzle 12 and operate the foot switch 39 and the control switch. These are all auxiliary tasks with low human intervention. Compared with the current manual tearing of the plastic culture bag 18 to remove the tissue culture seedlings for cleaning, the work efficiency is greatly improved, the labor cost is low, and the tissue culture seedlings are not damaged, effectively reducing customer losses and having good practicality.

[0060] In this embodiment, the controller automatically controls a pair of grippers to loosen their grip on the lower part of the culture bag 18 according to the program. Then, it is observed by the naked eye whether all the tissue culture seedlings in the culture bag 18 have fallen into the water in the cleaning area 23. Once it is observed that all the tissue culture seedlings in the culture bag 18 have fallen into the water in the cleaning area 23, the foot switch 39 can be pressed again to repeat the aforementioned action to perform the tearing operation of the next culture bag 18.

[0061] In this embodiment, a conductive ring and a wire 6 are provided inside the tube body 5. The wire 6 extends from the lower part of the tube body 5. The conventional technology of the conductive ring and the wire 6 is used to transmit electrical signals and electrical energy to the air pump, control valve 13 and other electrical components on the rotating turntable 11. With this design, even if the turntable 11 rotates, it will not affect the conductivity and control signal transmission of the air pump and control valve 13 on the turntable 11.

[0062] In this embodiment, as Figure 2 As shown, the chassis 1 is fixedly installed on the ground 41 via the base plate 10.

[0063] In this embodiment, as Figure 1 As shown, the tissue culture seedling cleaning device includes multiple cleaning boxes 20 arranged in a straight line. Adjacent cleaning boxes 20 are connected via connectors 40, and the multiple cleaning boxes 20 can move along their arrangement direction; preferably, as shown... Figure 2 As shown, the bottom of the cleaning tank 20 is provided with wheels 37, and a track 38 is provided below the wheels 37. The track 38 is fixedly installed on the ground 41, and the wheels 37 roll along the track 38. This design ensures that multiple cleaning tanks 20 always move along their arrangement direction. In this embodiment, the movement of the wheels 37 of multiple cleaning tanks 20 on the track 38 can be achieved by setting up a tractor to tow multiple sections of the cleaning tank 20, similar to a train. The position of the cleaning tank 20 on the track 38 is controlled by controlling the start and stop of the tractor. Preferably, the track 38 is connected end to end to form a closed loop. This design helps to improve cleaning efficiency.

[0064] In this embodiment, as Figure 1 and Figure 2 As shown, each cleaning tank 20 is equipped with a partition 21, which divides the cleaning tank 20 into a liquid storage area 22 and a cleaning area 23. A drain pipe 24 is provided at the bottom of the cleaning area 23, and a control valve 25 is installed on the drain pipe 24. When the control valve 25 is opened, the water in the cleaning area 23 flows out and is discharged through the drain pipe 24.

[0065] Preferred, such as Figure 1 and Figure 2 As shown, a drainage ditch 26 is provided on the ground 41. The drainage ditch 26 is located directly below the drain pipe 24 and extends along the length of the track 38. With this design, as the multiple cleaning boxes 20 move along their arrangement direction, the water discharged from the drain pipe 24 can always fall into the drainage ditch 26 and not flow around.

[0066] In this embodiment, as Figure 1 and Figure 2 As shown, the cleaning zone 23 is equipped with an elastic mesh plate 33 that protrudes upwards in the center. The elastic mesh plate 33 is elastic and can be bent, such as... Figure 2 As shown, the elastic mesh plate 33 is located below the water surface 34 in the cleaning zone 23. With this design, after the culture bag 18 is torn open, the tissue culture seedlings fall into the cleaning zone 23 and onto the water surface 34 without colliding with the elastic mesh plate 33, effectively protecting the safety of the tissue culture seedlings and preventing them from being injured.

[0067] In this embodiment, as Figure 1As shown, the projection of the elastic mesh plate 33 on the bottom of the cleaning area 23 covers the bottom of the cleaning area 23. With this design, when the water in the cleaning area 23 is drained, all the tissue culture seedlings fall on the upper surface of the elastic mesh plate 33, and there will be no tissue culture seedlings below the elastic mesh plate 33.

[0068] In this embodiment, as Figure 1 and Figure 2 As shown, connecting plates 32 are fixedly installed at opposite ends of the elastic mesh plate 33. Preferably, the connecting plates 32 are inverted L-shaped, with their lower ends attached to the inner wall of the cleaning tank 20, and their upper ends extending out of the cleaning tank 20 and hinged to it. A lifting device 31 is also connected to the upper end of the connecting plate 32, and the lifting device 31 is fixedly connected to the cleaning tank 20. This design allows the connecting plates 32 to rotate back and forth around their hinge point with the cleaning tank 20 by controlling the lifting device 31, thus moving the elastic mesh plate 33 up and down and changing the degree of protrusion of the elastic mesh plate 33. This achieves the separation of the tissue culture seedlings from the water surface 34 and controls the elastic mesh plate 33. The tissue culture seedlings are lowered and reset, falling back into the water. This cycle is repeated, with the elastic mesh plate 33 moving up and down multiple times to lift the seedlings off the water surface 34 and then back into the water. The water in the cleaning zone 23 continuously and protectively pats the seedlings, gradually separating the clustered seedlings into independent seedlings. This design facilitates the subsequent cleaning of the substrate from the seedlings and makes it easier to plant them in the ground after cleaning. It eliminates the hassle of manually separating the clustered seedlings, saving time and effort, greatly improving work efficiency, reducing labor costs, and preventing damage to the seedlings, effectively reducing customer losses and demonstrating good practicality.

[0069] In this embodiment, each time the tissue culture seedling falls into the water, it will have a soft collision with the water surface 34. This collision will not damage the tissue culture seedling. It is equivalent to the water in the cleaning zone 23 continuously and protectively patting the tissue culture seedling. At the same time, the multiple collisions cause the multiple tissue culture seedlings that are clustered together to gradually disperse and form independent tissue culture seedlings that are not connected to each other. During this process, the water in the cleaning zone 23 will also perform an initial cleaning of the tissue culture seedling, washing away some of the substrate.

[0070] In this embodiment, as Figures 1-4 As shown, several rinsing pipes 27 are provided above and below the elastic mesh plate 33 in the cleaning zone 23. Multiple nozzles 28 are spaced along the length of each rinsing pipe 27. These nozzles 28 can spray and rinse all areas of the upper and lower surfaces of the elastic mesh plate 33 and the connecting plate 32 located within the cleaning zone 23. Figure 3As shown, when the connecting plate 32 is inverted L-shape and its lower end is attached to the inner wall of the cleaning box 20, the multiple nozzles 28 on the multiple rinsing pipes 27 spray and rinse all areas of the upper and lower surfaces of the elastic mesh plate 33 and the lower end of the connecting plate 32. The design of multiple rinsing pipes 27 can ensure that the tissue culture seedlings on the upper surface of the elastic mesh plate 33 are rinsed in an all-round and thorough manner, completely washing away the substrate and thoroughly cleaning the tissue culture seedlings. The design of the tissue culture seedling cleaning device to clean the tissue culture seedlings twice ensures a good cleaning effect.

[0071] Preferred, such as Figures 1-4 As shown, several rinsing pipes 27 above the elastic mesh plate 33 are distributed at the edge of the cleaning area 23. This design does not affect the rotation of the connecting plate 32, and can prevent tissue culture seedlings falling into the cleaning area 23 from colliding with the rinsing pipes 27 and causing damage to the tissue culture seedlings, thus effectively protecting the safety of the tissue culture seedlings.

[0072] In this embodiment, as Figure 1 and Figure 4 As shown, the flushing pipe 27 extends into the liquid storage area 22 through the sealed partition 21, and is connected to the water pump 30 in the liquid storage area 22 through the connecting pipe 29. After the water pump 30 is started, the water pump 30 draws water from the liquid storage area 22 and sends it to the flushing pipe 27.

[0073] In this embodiment, as Figure 1 and Figure 2 As shown, the tissue culture seedling cleaning device also includes a water inlet pipe 35, which is located above the cleaning tank 20. The water inlet pipe 35 is equipped with two control valves 36, which control the water inlet pipe 35 to add water to the liquid storage area 22 and the cleaning area 23 respectively.

[0074] In this embodiment, as Figure 1 and Figure 2 As shown, when the tissue culture seedling cleaning device is working, firstly, control valve 36 is opened to add water to the liquid storage area 22 and cleaning area 23 of the cleaning tank 20. Then, the cleaning area 23 of the cleaning tank 20 is moved to directly below the suction nozzle 12 in the suction bag position. Here, the control motor 9 can drive the turntable 11 to rotate, so that the suction nozzle 12 moves to the suction bag position and the cleaning area 23 of the cleaning tank 20 moves to directly below the suction nozzle 12 in the suction bag position, so that the action is linked and synchronized. That is, the foot switch 39 controls the motor 9 to rotate at a certain angle to move the suction nozzle 12 to the suction bag position one by one and move the cleaning area 23 of the cleaning tank 20 to directly below the suction nozzle 12 in the suction bag position one by one. With this design, only one step of the foot switch 39 is needed to make the motor 9 drive the turntable 11 to rotate, so that the next suction nozzle 12 moves to the suction bag position and the next cleaning tank 20 moves to directly below the suction nozzle 12 in the suction bag position.

[0075] In addition, the opening of control valve 36 to add water to the liquid storage area 22 and cleaning area 23 of the cleaning tank 20 can also be synchronously controlled via foot switch 39, such as... Figure 1 As shown, stepping on the foot switch 39 once causes the motor 9 to drive the turntable 11 to rotate, moving the next suction nozzle 12 to the suction bag position and the next cleaning tank 20 to directly below the suction nozzle 12 in the suction bag position. At the same time, the control valve 36 opens to add water to the liquid storage area 22 and the cleaning area 23 of the next cleaning tank 20. The amount of water added can be controlled by the controller. Since the water flow rate and water addition time of the control valve 36 are constant, the controller controls the water addition by controlling the opening time of the control valve 36. When the water is added, the controller automatically controls the control valve 36 to close.

[0076] In this embodiment, when the controller detects the activation of the foot switch 39, it indicates that the motor 9 drives the turntable 11 to rotate, causing the next suction nozzle 12 to move to the suction bag position and the next cleaning tank 20 to move directly below the suction nozzle 12 in the suction bag position to perform the tearing operation of the next culture bag 18. At this time, the controller will control the cleaning tank 20, which has just left directly below the suction nozzle 12 in the suction bag position, according to the preset control program. That is, the lifting device 31 on the previous cleaning tank 20 will move, causing the elastic mesh plate 33 to move up and down, so that the tissue culture seedlings are repeatedly lifted off the water surface 34 and then fall back into the water. The water in the cleaning area 23 continuously and protectively beats the tissue culture seedlings, causing the multiple tissue culture seedlings that are clustered together to gradually disperse and form independent tissue culture seedlings that are not connected to each other. The duration of the lifting device 31 is set by the program. After the duration is reached, the controller controls the lifting device 31 to stop moving and simultaneously controls the control valve 25 to open, so that the water in the cleaning area 23... Water flows out through drain pipe 24 and is discharged. After a period of time, the water in the cleaning area 23 is drained. The controller continues to control the water pump 30 to start and draw water from the storage area 22 according to the control program and send it to the flushing pipe 27. The tissue culture seedlings on the upper surface of the elastic mesh plate 33 are thoroughly rinsed through the nozzle 28, removing the substrate and cleaning the tissue culture seedlings completely. The water sprayed from the nozzle 28 is also discharged from the drain pipe 24 and flows away into the drainage ditch 26. It can be seen that during the operation of the tissue culture seedling cleaning device, it is only necessary to manually control the next cleaning box 20 to move directly below the suction nozzle 12 in the suction bag position through the foot switch 39. It is an auxiliary operation and does not require manual cleaning of tissue culture seedlings. The degree of human intervention is low. Compared with the current method of manually tearing open the plastic culture bag 18 to take out the tissue culture seedlings for cleaning, the work efficiency is greatly improved, the labor cost is low, and the tissue culture seedlings are not damaged, which effectively reduces customer losses and has good practicality.

[0077] Furthermore, this aspect also proposes a method for removing the tissue culture seedling culture bag 18 and cleaning the substrate without causing damage, wherein the following steps are performed using any of the aforementioned apparatus for removing the tissue culture seedling culture bag 18 and cleaning the substrate without causing damage:

[0078] S1. Control motor 9 to drive turntable 11 to rotate, causing nozzle 12 to move to suction bag position;

[0079] S2. Add water to the liquid storage area 22 and the cleaning area 23 of the cleaning tank 20;

[0080] S3. Control the cleaning box 20 to move directly below the suction nozzle 12 in the suction bag position;

[0081] S4. Contact the culture bag 18 with the suction nozzle 12 in the suction bag position, and use the suction nozzle 12 to hold and lift the culture bag 18.

[0082] S5. Control the cylinder to drive a pair of grippers closer together, then control the pair of grippers to clamp the lower ends of the culture bag 18 respectively. Then control the cylinder to drive the pair of grippers to move away from each other and tear open the lower part of the culture bag 18. The tissue culture seedlings in the culture bag 18 fall into the water in the cleaning area 23, while the upper part of the culture bag 18 remains sucked by the suction nozzle 12.

[0083] S6. Control a pair of grippers to release the clamping of the culture bag 18, and then repeat S1-S5 to move the next suction nozzle 12 to the suction bag position and move the next water-filled washing tank 20 directly below the suction nozzle 12 in the suction bag position.

[0084] S7. The tissue culture seedlings float in the water. The lifting device 31 is controlled to move the elastic mesh plate 33 upward and increase the degree of protrusion of the elastic mesh plate 33, so that the elastic mesh plate 33 lifts the tissue culture seedlings and separates them from the water surface 34. Then the elastic mesh plate 33 is controlled to descend and reset, and the tissue culture seedlings fall into the water again and collide with the water surface 34. By controlling the elastic mesh plate 33 to move up and down multiple times, the water in the cleaning area 23 continuously and protectively beats the tissue culture seedlings until the multiple tissue culture seedlings that have gathered together are dispersed.

[0085] S8. Drain the water in the cleaning area 23, control the nozzle 28 to spray water onto the tissue culture seedlings scattered on the elastic mesh plate 33 to rinse the tissue culture seedlings a second time, wash away the substrate on the tissue culture seedlings, and drain the cleaning water away from the drain pipe 24 in real time.

[0086] S9. When the empty culture bag 18 below the suction nozzle 12 moves directly above the bag holding box 19, control the suction nozzle 12 to release the suction on the culture bag 18, and the culture bag 18 falls into the bag holding box 19.

[0087] Preferably, in this embodiment, S1-S3 and S9 are linked and controlled synchronously. For example, stepping on the foot switch 39 controls the motor 9 to rotate the turntable 11, moving the next suction nozzle 12 to the suction bag position, opening the control valve 36 to add water to the storage area 22 and cleaning area 23 of the next cleaning tank 20, moving the next cleaning tank 20 directly below the suction nozzle 12 in the suction bag position, and closing the control valve 13 of the suction nozzle 12 rotated to the top of the bag holding box 19. This design can further shorten the time for non-damaging removal of tissue culture seedling culture bags 18 and cleaning of the substrate, and further improve work efficiency. At the same time, S5-S8 can be automatically controlled by the set control program to control the action of the device for non-damaging removal of tissue culture seedling culture bags 18 and cleaning of the substrate in this embodiment. Only slight human intervention is required during the work process, resulting in high work efficiency, low labor costs, and no damage to the tissue culture seedlings, effectively reducing customer losses and making it highly practical.

[0088] In this embodiment, after S8, the scattered tissue culture seedlings on the elastic mesh plate 33 can be manually removed.

[0089] In this embodiment, as Figure 1 As shown, when the tracks 38 are connected end to end to form a closed loop, the tractor head can drive multiple cleaning boxes 20 back to the suction bag position. This design helps to improve cleaning efficiency.

[0090] The above description is merely a preferred embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformations made using the contents of the present invention's specification and drawings under the inventive concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A device for removing tissue culture seedling culture bags and cleaning the substrate without causing damage, characterized in that, The culture bag tearing device is used for tearing the culture bag, and the tissue culture seedling cleaning device is used for cleaning the tissue culture seedlings. The culture bag tearing device comprises: A machine box is vertically provided with a pipe body, and a motor is arranged in the machine box and is in transmission connection with the pipe body. A rotating disc is coaxially fixed to the upper end of the pipe body, and the lower surface of the rotating disc is uniformly provided with a plurality of suction nozzles. A tearing mechanism is arranged below the suction nozzles and is fixedly connected with the machine box. The tearing mechanism comprises a gas cylinder and a pair of clamping jaws arranged on the gas cylinder. The pair of clamping jaws are driven by the gas cylinder to move close to or away from each other. The clamping jaws can clamp a corner of the culture bag. The tissue culture seedling cleaning device comprises a plurality of cleaning boxes arranged in a linear form.

2. The apparatus for removing the tissue culture bag and washing the medium without damage according to claim 1, wherein, Each cleaning box is provided with a partition plate II. The partition plate II divides the cleaning box into a liquid storage area and a cleaning area.

3. The apparatus for removing the tissue culture bag and washing the medium without damage according to claim 1, wherein, The bottom of the cleaning area is provided with a drain pipe, and the drain pipe is provided with a control valve II.

4. The apparatus for removing the tissue culture bag and washing the medium without damage according to claim 1, wherein, The cleaning area is provided with an elastic mesh plate protruding upward in the middle.

5. The apparatus for removing the tissue culture bag and washing the medium without damage according to claim 1, wherein, The elastic mesh plate is located below the water surface in the cleaning area. The projection of the elastic mesh plate on the bottom of the cleaning area covers the bottom of the cleaning area. The opposite ends of the elastic mesh plate are fixedly provided with connecting plates. The upper end of the connecting plate extends out of the cleaning box and is hingedly connected with the cleaning box. The upper end of the connecting plate is also connected with a lifting device. The lifting device is fixedly connected with the cleaning box. The lifting device drives the connecting plate to rotate around the hinge position with the cleaning box to drive the elastic mesh plate to move up and down and change the protruding degree of the elastic mesh plate. The upper and lower parts of the elastic mesh plate in the cleaning area are provided with a plurality of washing pipes. The washing pipes are provided with a plurality of nozzles at intervals. The nozzles on the plurality of washing pipes spray and wash all areas of the upper and lower surfaces of the elastic mesh plate and the connecting plate located in the cleaning area. The washing pipes extend through the partition plate II into the liquid storage area and are in communication with the water pump in the liquid storage area. The upper end of the machine box is a detachable box cover. The machine box is provided with a partition plate I. The lower end of the pipe body extends through the box cover and the partition plate I and is rotatably connected with the box cover and the partition plate I. The lower surface of the box cover and the upper surface of the partition plate I are provided with stop rings. The stop rings are coaxially fixedly connected with the pipe body. The machine box is provided with a motor below the partition plate I. The motor is in transmission connection with a gear and a gear ring. The gear ring is coaxially fixedly connected with the lower end of the pipe body. The suction pump is fixedly connected with the rotating disc. The pipe comprises a suction pipe, a ring pipe and a connecting pipe. The suction end of the suction pump is connected with the ring pipe through the suction pipe. Each connecting pipe is provided with a control valve I. The culture bag tearing device further comprises a bag containing box. The bag containing box is arranged below the suction nozzles. The connecting plate is in inverted L shape. The lower end of the connecting plate is attached to the inner wall of the cleaning box.

6. The apparatus for removing the tissue culture bag and washing the medium without damage according to claim 1, wherein, The bottom of the cleaning box is provided with wheels, and the wheels are provided below a track along which the wheels roll and travel. A drain ditch is provided directly below the drain pipe and extends along the length direction of the track.

7. The apparatus for removing the tissue culture bag and washing the medium without damage according to claim 1, wherein, The tissue culture seedling cleaning device further comprises a water supply pipe arranged above the cleaning box, and two control valves three are arranged on the water supply pipe to control water supply to the liquid storage area and the cleaning area.

8. A method for removing a tissue culture bag and washing a medium without damage, characterized by, The following steps are performed by using the device for removing a tissue culture seedling culture bag and cleaning substrate without damage according to any one of claims 1-7: S1, control the motor to drive the rotating disc to rotate to move the suction nozzle to the bag suction position; S2, supply water to the liquid storage area and the cleaning area of the cleaning box; S3, control the cleaning box to move to the position directly below the suction nozzle at the bag suction position; S4, contact the culture bag with the suction nozzle at the bag suction position, and lift the culture bag by the suction nozzle; S5, control the cylinder to drive a pair of clamping jaws to approach each other, then control the pair of clamping jaws to clamp the lower two ends of the culture bag, then control the cylinder to drive the pair of clamping jaws to move away from each other to tear the lower part of the culture bag, and the tissue culture seedlings in the culture bag fall into the water in the cleaning area, and the upper part of the culture bag is kept being sucked by the suction nozzle; S6, control the pair of clamping jaws to loosen the clamping of the culture bag, and then repeat S1-S5 to move the next suction nozzle to the bag suction position and move the next cleaning box to the position directly below the suction nozzle at the bag suction position; S7, the tissue culture seedlings float in the water, control the lifting device to drive the elastic mesh plate to move upwards and increase the convexity of the elastic mesh plate, so that the elastic mesh plate lifts the tissue culture seedlings and separates them from the water surface, then control the elastic mesh plate to move downwards and reset, and the tissue culture seedlings fall into the water again, and the water in the cleaning area continuously protects and splashes the tissue culture seedlings by controlling the elastic mesh plate to move upwards and downwards multiple times, until the multiple tissue culture seedlings gathered together are dispersed; S8, empty the water in the cleaning area, control the spray head to spray water to the dispersed tissue culture seedlings on the elastic mesh plate to rinse the tissue culture seedlings twice and clean the substrate on the tissue culture seedlings; S9, when the empty culture bag below the suction nozzle moves to the position directly above the bag containing box, control the suction nozzle to loosen the suction of the culture bag, and the culture bag falls into the bag containing box.

9. The method for removing tissue culture seedling culture bags and cleaning the substrate without damage as described in claim 8, characterized in that, S1-S3 and S9 are controlled to be performed synchronously.

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