Pathogen detection device and detection method combining isothermal amplification and fluorescence immunochromatography

Abstract

The present invention provides a pathogen detection device and detection method combining isothermal amplification and fluorescent immunochromatography, including an isothermal amplification nucleic acid chromatography chip and a chip detector; the isothermal amplification nucleic acid chromatography chip is used for isothermal amplification reaction and fluorescent immunochromatography of nucleic acid samples, and the chip detector is used to control the temperature of the chip, drive sample movement, and read chromatography results. The device of the present invention integrates isothermal amplification and nucleic acid chromatography to achieve fully automatic detection from sample processing to result output without the need for professional operators; the entire detection process can be completed within 30 minutes, meeting the needs of rapid detection; high-efficiency isothermal amplification technology is used, combined with high-sensitivity fluorescence detection, to ensure that the results are accurate and reliable. The device of the present invention can detect multiple nucleic acid viruses at one time by setting a pathogen isothermal amplification chamber, thereby improving detection efficiency and reducing detection costs.

Description

Technical Field

[0001] The present invention belongs to the field of POCT rapid detection, and particularly relates to a pathogen detection device and a detection method combining isothermal amplification and fluorescent immunochromatography. Background Art

[0002] The development direction of nucleic acid detection technology is informatization, convenience, simplification and automation. Multi-target, convenient, fast and easy-to-popularize detection technology has become a major demand for pathogen detection, epidemic prevention and control at home and abroad.

[0003] Immunochromatographic antigen detection technology has the advantages of low cost and convenience, but it lacks sensitivity and cannot be used for pathogen detection that lacks antibodies, let alone for typing mutant pathogens; PCR detection technology has high sensitivity but relies on expensive repeat instruments, consumes a lot of social resources and limits most usage scenarios; emerging temperature amplification technologies such as LAMP and RPA have greatly reduced the dependence of nucleic acid amplification on equipment, but still need to be used in conjunction with traditional PCR detectors, and the usage scenarios are limited.

[0004] The existing technology is a Chinese patent publication number CN218584658U, entitled "Intelligent Disposable Detection Device," which includes a housing with a spectral sensing chip; a test strip or test chamber provided within the housing, the test strip or test chamber having a sample loading area, and the housing having a sample loading hole corresponding to the sample loading area; the spectral sensing chip having a photosensitive receiving unit; the photosensitive receiving unit facing the interior of the housing; an excitation light source provided within the housing, the controlled end of the excitation light source connected to the control end of the spectral sensing chip; the data output end of the spectral sensing chip connected to an external control device via a connecting cable; and the power source for the spectral sensing chip and the excitation light source is the external control device. This intelligent disposable detection device lacks a chip reaction site, so a separate reaction site must be set up, making it incapable of portable detection. Furthermore, the intelligent disposable detection device can only detect one nucleic acid virus at a time, reducing detection efficiency and increasing the cost of nucleic acid virus detection.

[0005] Therefore, a highly sensitive, low-cost, fast and convenient nucleic acid detection technology is developed for screening and monitoring in multiple scenarios such as hospitals, communities, high-risk lockdown areas, customs and homes. Summary of the Invention

[0006] The technical problem to be solved by the present invention is how to reduce costs, conveniently and accurately perform full-automatic detection of multiple pathogens.

[0007] The present invention solves the above technical problems through the following technical means:

[0008] The first aspect of the present invention provides a pathogen detection device combining isothermal amplification and fluorescent immunochromatography, comprising an isothermal amplification nucleic acid chromatography chip and a chip detector;

[0009] The isothermal amplification nucleic acid chromatography chip is used for isothermal amplification reaction of nucleic acid samples and fluorescent immunochromatography, and the chip detector is used to control the temperature of the chip, drive the sample movement, and read the chromatography results;

[0010] The isothermal amplification nucleic acid chromatography chip comprises a power cavity, a pathogen isothermal amplification cavity, a premixing cavity and a fluorescent immunochromatographic test strip; the power cavity, pathogen isothermal amplification cavity, premixing cavity and fluorescent immunochromatographic test strip are connected in sequence;

[0011] The chip detector includes a temperature control module, a chip driving module, a fluorescence signal reading module, a microcomputer and operating software; the microcomputer is connected to the temperature control module, the fluorescence signal reading module and the chip driving module in sequence through a digital or analog interface.

[0012] Beneficial effects: The device of the present invention integrates isothermal amplification and nucleic acid chromatography to achieve fully automatic detection from sample processing to result output, without the need for professional operators; the entire detection process can be completed within 30 minutes, meeting the needs of rapid detection; the use of high-efficiency isothermal amplification technology combined with high-sensitivity fluorescence detection ensures accurate and reliable results. The device of the present invention can detect multiple nucleic acid viruses at one time by setting up a pathogen isothermal amplification chamber, thereby improving detection efficiency and reducing detection costs.

[0013] Preferably, the power chamber includes a cavity and a pressure rod, and the pressure rod is arranged on the cavity.

[0014] Preferably, the pathogen isothermal amplification chamber contains nucleic acid primers, isothermal amplification enzymes and isothermal amplification reagents.

[0015] Preferably, a test strip loading solution is provided inside the pre-mixing chamber, and the test strip loading solution is a mixed solution of PBS and Tween reagent.

[0016] Preferably, the fluorescent immunochromatographic test strip is arranged inside the chip, and the fluorescent immunochromatographic test strip comprises a sample pad, a conjugation pad, and a nitrocellulose membrane, and the conjugation pad is provided with a composite fluorescent quantum dot probe.

[0017] Preferably, the temperature control module comprises a semiconductor refrigeration chip, a temperature sensor, and a heat sink; the semiconductor refrigeration chip is arranged between the temperature sensor and the heat sink.

[0018] Preferably, the chip driving module includes a stepping motor and a pressing device, and the stepping motor is connected to the pressing device via a connecting rod and a slider.

[0019] Preferably, the fluorescence signal reading module includes an excitation light source, a narrow-band filter and a camera; the excitation light source is arranged on both sides of the camera, and the narrow-band filter is arranged below the camera lens.

[0020] Preferably, the microcomputer is used to carry the operating software; the operating software includes a temperature control unit, a motor drive control unit, a camera acquisition control unit, an operating interface, a data storage unit, a temperature control algorithm and a test strip image analysis algorithm. The operating software is used to control the chip detector and the chip to execute the isothermal amplification fluorescence chromatography detection process and quantitatively read the fluorescence value on the test strip.

[0021] Preferably, the pathogen isothermal amplification chamber comprises multiple reaction chambers for simultaneously detecting multiple different pathogens.

[0022] Beneficial effects: The present invention sets up multiple reaction chambers, and the nucleic acid primers in each reaction chamber are different, so that different pathogens can be detected simultaneously. Therefore, the isothermal amplification nucleic acid chromatography chip can detect multiple pathogens at the same time, realizing high-throughput, multi-target detection, thereby reducing detection costs.

[0023] Preferably, the nitrocellulose membrane of the fluorescent immunochromatographic test strip is marked with multiple T lines, one C line and one internal reference line, and can be used for the simultaneous detection of multiple pathogens.

[0024] Preferably, the excitation light source is a 365nm excitation light source, the narrow-band filter is a 650nm narrow-band filter, and the camera is a CMOS camera.

[0025] Preferably, the fluorescent signal is a 650nm fluorescent signal.

[0026] Preferably, the microcomputer is a Raspberry Pi5 development board.

[0027] A second aspect of the present invention provides a detection method for a pathogen detection device combining isothermal amplification and fluorescent immunochromatography, comprising the following steps:

[0028] (1) Connect the chip detector to the power supply and start the operating software;

[0029] (2) Load the pathogen nucleic acid into the pathogen isothermal amplification chamber using a pipette, affix a sealing film, and place it in the chip detector;

[0030] (3) Click the test button on the operating software interface, enter the test page, enter the test number, and click the start button to automatically start the temperature control module to complete the isothermal amplification reaction of the pathogen-specific sequence;

[0031] (4) The fluorescence signal reading module automatically detects the fluorescence signal of the fluorescent immunochromatographic test strip and displays the fluorescence intensity curve. The microcomputer will save the detection data.

[0032] Preferably, the detection method of the pathogen detection device combining isothermal amplification and fluorescent immunochromatography can detect multiple pathogens simultaneously.

[0033] The advantages of the present invention are:

[0034] The device of the present invention integrates isothermal amplification and nucleic acid chromatography to achieve fully automatic detection from sample processing to result output, without the need for professional operators; the entire detection process can be completed within 30 minutes, meeting the needs of rapid detection; the use of high-efficiency isothermal amplification technology combined with high-sensitivity fluorescence detection ensures accurate and reliable results. The device of the present invention can detect multiple nucleic acid viruses at one time by setting up a pathogen isothermal amplification chamber, thereby improving detection efficiency and reducing detection costs.

[0035] The present invention provides multiple reaction chambers, each containing different nucleic acid primers, so that different pathogens can be detected simultaneously. Therefore, the isothermal amplification nucleic acid chromatography chip can detect multiple pathogens simultaneously, achieving high-throughput, multi-target detection, thereby reducing detection costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 This is a diagram of the structure of an isothermal amplification fluorescence chromatography chip;

[0037] Figure 2 This is the chip detector structure diagram;

[0038] Among them, 10 isothermal amplification fluorescence chromatography chip; 11-power chamber; 111-pressure rod; 112-cavity; 12-pathogen isothermal amplification chamber; 121-reaction chamber; 13-premixing chamber; 131-test strip sample solution; 14-fluorescent immunochromatographic test strip; 20-chip detector; 21-temperature control module; 22-fluorescence signal reading module; 221-excitation light source; 222-camera; 23-chip drive module; 231-pressing device; 232-stepping motor; 24-microcomputer. DETAILED DESCRIPTION

[0039] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0040] Example 1

[0041] Please refer to the attached Figure 1-2 This embodiment provides a pathogen detection device that combines isothermal amplification and fluorescent immunochromatography, including an isothermal amplification nucleic acid chromatography chip 10 and a chip detector 20; the isothermal amplification nucleic acid chromatography chip 10 is used for isothermal amplification reaction and fluorescent immunochromatography of nucleic acid samples, and the chip detector 20 is used to control the temperature of the chip, drive sample movement, and read chromatography results.

[0042] The isothermal amplification nucleic acid chromatography chip 10 comprises a power chamber 11, a pathogen isothermal amplification chamber 12, a premixing chamber 13 and a fluorescent immunochromatographic test strip 14; the power chamber 11, the pathogen isothermal amplification chamber 12, the premixing chamber 13 and the fluorescent immunochromatographic test strip 14 are connected in sequence.

[0043] The power chamber 11 includes a cavity 112 and a pressure rod 111, and the pressure rod 111 is arranged on the cavity 112; the cavity 112 is cylindrical in shape, and the pressure rod 111 compresses the cylindrical cavity 112, so that the air inside the cavity 112 drives the sample to move; the power chamber 11 is a piston push-pull structure, and the pressure rod 111 is provided with a sealing rubber sleeve.

[0044] In this embodiment, the pathogen isothermal amplification chamber 12 is provided with four reaction chambers 121 , each reaction chamber 121 contains an isothermal amplification enzyme, an isothermal amplification reagent and different nucleic acid primers, and can detect four different pathogens at the same time.

[0045] The shape of the reaction chamber 121 can be circular or rectangular. The four reaction chambers 121 are arranged in two rows, with two chambers in each row, forming a rectangular shape. The four reaction chambers 121 are arranged on a heating device (not shown) of the isothermal amplification nucleic acid chromatography chip 10. When the isothermal amplification nucleic acid chromatography chip 10 is inserted into the chip detector 20, the chip detector 20 heats the reaction chamber 121, thereby realizing local constant temperature heating of the pathogen isothermal amplification chamber 12 by the isothermal amplification nucleic acid chromatography chip 10.

[0046] A test strip sample solution 131 is provided inside the premixing chamber 13 . The test strip sample solution 131 is a mixed solution of PBS and Tween reagent. The sample needs to be fully mixed with the test strip sample solution 131 in the premixing chamber 13 .

[0047] The fluorescent immunochromatographic test strip 14 is arranged inside the chip. The fluorescent immunochromatographic test strip 14 contains a sample pad (not shown in the figure), a binding pad (not shown in the figure), and a nitrocellulose membrane (not shown in the figure). The binding pad is provided with a composite fluorescent quantum dot probe (not shown in the figure). The fluorescent immunochromatographic test strip 14 is used to draw 4 T lines, 1 C line and 1 internal reference line through the nitrocellulose membrane for the simultaneous detection of 4 pathogens.

[0048] The chip detector 20 includes a temperature control module 21, a chip driver module 23, a fluorescence signal reading module 22, a microcomputer 24 and operating software; the microcomputer 24 is connected to the temperature control module 21, the fluorescence signal reading module 22, and the chip driver module 23 in sequence through a digital or analog interface, and coordinates and controls the operation of each module according to the detection program to collect, process, analyze, store and display the detection signals and data.

[0049] The temperature control module 21 and the fluorescence signal reading module 22 are connected through the isothermal amplification nucleic acid chromatography chip 10, and the temperature inside the chip is monitored in real time through a thermocouple or thermistor, and the heating device of the heating chip is accurately controlled according to the feedback temperature signal.

[0050] The temperature control module 21 includes a semiconductor refrigeration chip (not shown in the figure), a temperature sensor (not shown in the figure), and a heat sink (not shown in the figure); the semiconductor refrigeration chip is arranged between the temperature sensor and the heat sink, wherein the semiconductor refrigeration chip is adhered to the heat sink through thermal grease, and the semiconductor refrigeration chip is controlled by an H-bridge circuit. When the current passes through the semiconductor refrigeration chip in the forward direction, the semiconductor refrigeration chip is in a heating state, and when the current passes through the semiconductor refrigeration chip in the reverse direction, the semiconductor refrigeration chip is in a cooling state.

[0051] The chip drive module 23 includes a stepper motor 232 and a pressing device 231. The stepper motor 232 is connected to the pressing device 231 via a connecting rod (not shown) and a slider (not shown). The movement of the stepper motor 232 drives the slider and the connecting rod, thereby driving the pressing device 231 to move up and down, driving the pressing rod 111 of the power chamber 11 on the isothermal amplification nucleic acid chromatography chip 10.

[0052] The chip driving module 23 works by inputting stable compressed air into the pressure control port (not shown) of the isothermal amplification nucleic acid chromatography chip 10. The air pressure will act on the sample inside the chip. When the chip is connected to two different positions, two pressure control ports will be generated. When a pressure difference is established, the sample will flow unidirectionally from the high-pressure end to the low-pressure end.

[0053] The chip driving module 23 is used in the isothermal amplification fluorescence chromatography chip 10 and the chip detector 20 to drive the sample to flow in microchannels, pipes, etc., and the driving mechanical device composed of a high-precision stepper motor 232 presses the power cavity 11 of the chip to generate a pressure gradient in its air pressure source, thereby driving the sample to flow unidirectionally in the channel.

[0054] The fluorescence signal reading module 22 includes an excitation light source 221 , a narrowband filter (not shown) and a camera 222 ; the excitation light source 221 is disposed on both sides of the camera 222 , wherein the narrowband filter is disposed below the lens of the camera 222 .

[0055] The fluorescence signal reading module 22 is an excitation light source 221 that illuminates the detection area of ​​the isothermal amplification nucleic acid chromatography chip 10 to excite a fluorescence signal that passes through a narrow-band filter and is photographed by a camera 222 and then input into a microcomputer 24 for analysis.

[0056] The excitation light source 221 illuminates the composite quantum dot label captured on the T line of the fluorescent immunochromatographic test strip 14 to generate a fluorescent signal, which is captured by the camera 222 through a narrow-band filter; the fluorescent signal is a 650nm fluorescent signal, the excitation light source 221 is a 365nm UV light source, the narrow-band filter is a 650nm narrow-band filter, and the camera 222 is a CMOS camera.

[0057] The microcomputer 24 is used to carry the operating software; the operating software includes a temperature control unit, a motor drive control unit, a camera acquisition control unit, an operating interface, a data storage unit, a temperature control algorithm, and a test strip image analysis algorithm. The operating software is used to control the chip detector 20 and the isothermal amplification nucleic acid chromatography chip 10 to execute the isothermal amplification fluorescence chromatography detection process and quantify the fluorescence value on the fluorescent immunochromatography test strip 14. The microcomputer 24 is a Raspberry Pi5 development board.

[0058] The working principle of the pathogen detection device combining isothermal amplification and fluorescence immunochromatography in this embodiment is as follows:

[0059] 1) Sample pretreatment: Pre-treat various clinical samples to extract and purify pathogen nucleic acids;

[0060] 2) Chip loading: Use a pipette to sample and accurately load four nucleic acid samples into the pathogen isothermal amplification chamber 12, and seal it with sealing film;

[0061] 3) Insert the isothermal amplification nucleic acid chromatography chip 10 into the chip detector 20, click the detection button on the operating software interface to start the chip detector detection program;

[0062] 4) Isothermal Nucleic Acid Amplification: The temperature control module 21 of the chip detector 20 precisely controls the temperature of the pathogen isothermal amplification chamber 12 through a semiconductor refrigeration chip. The primers in the isothermal amplification chamber 12 specifically recognize the template and perform an amplification reaction at a constant temperature to obtain a large amount of circular DNA products modified with recognition groups. This reaction lasts for 10 minutes.

[0063] 5) Product drive: After the amplification reaction time is over, the chip drive module 23 quantitatively compresses the pressure rod 111 of the power chamber 11, and presses the circular DNA products after isothermal amplification in the four reaction chambers 121 into the pre-mixing chamber 13 to mix with the test strip sample solution 131, and then stands for 30 seconds;

[0064] 6) Nucleic Acid Chromatography: The chip drive module 23 continues to quantitatively compress the pressure rod 111 of the power chamber 11, delivering the mixed fluid in the premixing chamber 13 to the sample pad of the immunochromatographic test strip 14. The circular DNA product migrates along the chromatography direction, binds to the fluorescent nanotag as it passes through the binding pad, and is captured by the corresponding pre-marked antibodies as it passes through four different T lines. The chromatography process lasts for 8 minutes.

[0065] 7) Signal Acquisition: After chromatography is completed, the chip detector 20 activates the laser light source 221 and camera 222. The UV light source irradiates the composite quantum dot label captured by the T line of the immunochromatographic test strip 14, stimulating 650nm red light. The camera 222 captures the image and analyzes the fluorescence value of the T line to perform qualitative and quantitative analysis of the sample. The microcomputer 24 saves the analysis results.

[0066] Example 2

[0067] This embodiment provides a detection method for a pathogen detection device combining isothermal amplification and fluorescent immunochromatography, comprising the following steps:

[0068] (1) Connect the chip detector 20 to the power supply and start the operation software;

[0069] (2) The nucleic acid sample is loaded into the pathogen isothermal amplification chamber 12 using a pipette, and then sealed with a film and placed in the chip detector 20;

[0070] (3) Click the detection button on the operating software interface, enter the detection page, enter the detection sequence number, and click the start button to automatically perform the temperature control module 21 to complete the isothermal amplification reaction of the pathogen-specific sequence;

[0071] (4) The fluorescence signal reading module automatically detects the fluorescence signal of the fluorescence immunochromatographic test strip 14 and displays the fluorescence intensity curve. The microcomputer 24 saves the detection data.

[0072] The detection method of the pathogen detection device combined with isothermal amplification and fluorescence immunochromatography in this embodiment can detect four pathogens at the same time.

[0073] The device of the present invention integrates isothermal amplification and nucleic acid chromatography to achieve fully automatic detection from sample processing to result output, eliminating the need for professional operators and reducing the cost of training professional personnel; the entire detection process can be completed within 30 minutes, meeting the needs of rapid detection; the use of high-efficiency isothermal amplification technology combined with high-sensitivity fluorescence detection ensures accurate and reliable results. The device of the present invention can detect multiple nucleic acid viruses at one time by setting up a pathogen isothermal amplification chamber, thereby improving detection efficiency and reducing detection costs.

[0074] The present invention provides multiple reaction chambers, each containing different nucleic acid primers, so that different pathogens can be detected simultaneously. Therefore, the isothermal amplification nucleic acid chromatography chip can detect four pathogens simultaneously, achieving high-throughput, multi-target detection, thereby reducing detection costs.

[0075] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A pathogen detection device combining isothermal amplification and fluorescent immunochromatography, characterized in that: It includes an isothermal amplification nucleic acid chromatography chip and a chip detector; the isothermal amplification nucleic acid chromatography chip is used for isothermal amplification of pathogenic nucleic acids and fluorescent immunochromatography, and the chip detector is used to control the temperature of the chip, drive liquid movement, and read the chromatography results; The isothermal amplification nucleic acid chromatography chip comprises a power chamber, a pathogen isothermal amplification chamber, a premixing chamber, and a fluorescent immunochromatographic test strip; the power chamber, pathogen isothermal amplification chamber, premixing chamber, and fluorescent immunochromatographic test strip are sequentially connected; the chip detector comprises a temperature control module, a chip driver module, a fluorescent signal reading module, a microcomputer, and operating software; the microcomputer is sequentially connected to the temperature control module, the fluorescent signal reading module, and the chip driver module via a digital or analog interface; The power chamber includes a cavity and a pressure rod, which is arranged on the cavity; the pathogen isothermal amplification chamber, which contains nucleic acid primers, isothermal amplification enzymes and isothermal amplification reagents; the pre-mixing chamber is provided with a test strip sample solution, which is a mixed solution of PBS and Tween reagent; the fluorescent immunochromatographic test strip is arranged inside the chip, and the fluorescent immunochromatographic test strip contains a sample pad, a binding pad, and a nitrocellulose membrane, and the binding pad is provided with a composite fluorescent quantum dot probe; The temperature control module includes a semiconductor refrigeration chip, a temperature sensor, and a heat sink; the semiconductor refrigeration chip is arranged between the temperature sensor and the heat sink; the chip drive module includes a stepper motor and a pressing device, and the stepper motor is connected to the pressing device through a connecting rod and a slider; the fluorescence signal reading module includes an excitation light source, a narrow-band filter, and a camera; the excitation light source is arranged on both sides of the camera, and the narrow-band filter is arranged below the camera lens; The microcomputer is used to carry the operating software; the operating software includes a temperature control unit, a motor drive control unit, a camera acquisition control unit, an operating interface, a data storage unit, a temperature control algorithm and a test strip image analysis algorithm. The operating software is used to control the chip detector and the chip to execute the isothermal amplification fluorescence chromatography detection process and quantitatively read the fluorescence value on the test strip.

2. The pathogen detection device combining isothermal amplification and fluorescence immunochromatography according to claim 1, characterized in that: The pathogen isothermal amplification chamber contains multiple reaction chambers for detecting multiple different pathogens at the same time.

3. The pathogen detection device combining isothermal amplification and fluorescence immunochromatography according to claim 1, characterized in that: The nitrocellulose membrane of the fluorescent immunochromatographic test strip is marked with multiple T lines, one C line and one internal reference line for the simultaneous detection of multiple pathogens.

4. The pathogen detection device combining isothermal amplification and fluorescence immunochromatography according to claim 1, characterized in that: The excitation light source is a 365 nm UV light source, the narrow-band filter is a 650 nm narrow-band filter, and the camera is a CMOS camera.

5. The pathogen detection device combining isothermal amplification and fluorescence immunochromatography according to claim 1, characterized in that: The fluorescence signal is 650nm.

6. The pathogen detection device combining isothermal amplification and fluorescence immunochromatography according to claim 1, characterized in that: The microcomputer is a Raspberry Pi5 development board.

7. A detection method of the pathogen detection device combining isothermal amplification and fluorescence immunochromatography according to any one of claims 1 to 6, characterized in that: The following steps are involved: (1) Connect the chip detector to the power supply and start the operating software; (2) Use a pipette to load the pathogen nucleic acid into the pathogen isothermal amplification chamber, affix a sealing film, and place it in the chip detector; (3) Click the detection button on the operating software interface, enter the detection page, enter the detection sequence number, and click the start button to automatically start the temperature control module to complete the isothermal amplification reaction of the pathogen-specific sequence; (4) The fluorescence signal reading module automatically detects the fluorescence signal of the fluorescent immunochromatographic test strip and displays the fluorescence intensity curve. The microcomputer will save the detection data.

8. The detection method according to claim 7, characterized in that The pathogen detection device combining isothermal amplification and fluorescent immunochromatography can detect multiple pathogens at the same time.

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