Plant extract for reducing sebum secretion, and preparation method and application thereof

Abstract

The application discloses a plant extract for reducing oil secretion, which comprises 5-25 parts of citrus by weight, 3-15 parts of sophora, 3-15 parts of compositae, 3-15 parts of seaweed plant and 1-10 parts of camellia by weight; and the preparation raw material further comprises a solvent. The grapefruit, sophora and dandelion are combined to inhibit 5alpha-reductase activity from the source, inhibit sebaceous gland cell activity and reduce skin oil secretion. The large leaf seaweed and green tea are combined to have good antioxidant effect, can relieve the irritation of peroxide squalene to the skin caused by skin lipid oxidation, and avoid inflammation; meanwhile, the moisturizing component contained can reduce skin water loss, avoid excessive oil secretion caused by dry skin, and solve the problems that the existing oil control skin care and color cosmetic products cannot fundamentally solve oil secretion, have obvious side effects, have insufficient effect, and are prone to recurrence.

Description

TECHNICAL FIELD

[0001] The present application relates to a plant extract for reducing sebum secretion and its preparation method and application, and relates to the field of A61K, in particular to the field of dressing configuration. BACKGROUND

[0002] Oily skin is a common skin condition, especially during puberty, excessive secretion of skin oil can block pores, causing pores to enlarge, and excess sebum provides nutrition for bacteria on the surface of the skin, causing skin inflammation. The existing methods for inhibiting the secretion of skin oil include inorganic powder physical adsorption method, cooling agent inhibition method, and single action of plant extract. Using inorganic powder to physically adsorb the oil on the surface of the skin has a quick effect but can easily block pores and cannot fundamentally solve the problem of excessive secretion of skin oil. The cooling agent has a short-term effect on the inhibition of oil, and the existing plant extract generally controls oil from a single aspect and cannot achieve long-term and comprehensive oil control. Therefore, it is crucial to develop a method for inhibiting the secretion of skin oil from multiple aspects while reducing skin irritation.

[0003] Chinese invention patent CN201811049512.1 discloses a green tea mask powder with blackhead removal effect and its preparation method, which contains green tea powder and has the effects of oil control, astringency, and acne mark lightening, and contains trehalose for moisturizing and hydration, but the oil control effect is not good for skin with severe oiliness, and the skin inflammation problem caused by oil cannot be effectively improved. Chinese invention patent CN202210594122.2 discloses a microemulsion composition of oleanolic acid and its preparation method and application, which prepares essential oil of plant extract into a liposome carrier to achieve good transdermal absorption effect and has an antioxidant effect, but the effect of inhibiting oil secretion is not obvious. SUMMARY

[0004] In order to develop a method for inhibiting the secretion of skin oil from multiple aspects, the first aspect of the present application provides a plant extract for reducing sebum secretion, which includes 5-25 parts of citrus fruit, 3-15 parts of Sophora plant, 3-15 parts of chrysanthemum plant, 3-15 parts of seaweed plant, and 1-10 parts of Camellia plant by weight, and the preparation raw material further includes a solvent.

[0005] As a preferred embodiment, the plant raw material includes 10-20 parts of citrus fruit, 5-10 parts of Sophora plant, 5-10 parts of chrysanthemum plant, 5-10 parts of seaweed plant, and 3-8 parts of Camellia plant by weight.

[0006] As a preferred embodiment, the plant raw material includes 15 parts of citrus fruit, 6 parts of Sophora plant, 6 parts of chrysanthemum plant, 6 parts of seaweed plant, and 5 parts of Camellia plant by weight.

[0007] As a preferred embodiment, the plant raw material comprises 8 parts of Citrus fruit, 9 parts of Sophora fruit, 10 parts of Compositae plant, 5 parts of seaweed plant, and 6 parts of Camellia plant.

[0008] As a preferred embodiment, the Citrus fruit is selected from one or a combination of several of the following: grapefruit, small red orange, lemon, bergamot, lime, and citron.

[0009] As a preferred embodiment, the Citrus fruit is grapefruit (Citrus x aurantium Linnaeus).

[0010] As a preferred embodiment, the Compositae plant is selected from one or a combination of several of the following: Taraxacum mongolicum Hand.-Mazz, dandelion, daisy, false small beak chrysanthemum, hetero-rostrate chrysanthemum, and powder chrysanthemum.

[0011] As a preferred embodiment, the Compositae plant is Taraxacum mongolicum Hand.-Mazz.

[0012] As a preferred embodiment, the Sophora fruit is Sophora flavescens Aiton; the seaweed plant is Zostera marina; and the Camellia plant is Camellia sinensis.

[0013] The second aspect of the present application provides a method for preparing a plant extract for reducing oil secretion, comprising the following steps:

[0014] S1. Citrus fruit, Sophora fruit, Compositae plant, seaweed plant, and Camellia plant are mixed and weighed, pretreated, added with solvent, and subjected to temperature-controlled directional wall-breaking extraction to obtain a plant crude extract.

[0015] S2. The plant crude extract is sequentially subjected to centrifugation, purification, and concentration to obtain a plant extract.

[0016] As a preferred embodiment, the ratio of the plant raw material to the solvent is 1:(15-40); and the extraction temperature during the wall-breaking extraction is 15-40℃.

[0017] As a preferred embodiment, the solvent is selected from one or a combination of two of the following: pure water and low alcohol.

[0018] As a preferred embodiment, the solvent is a solution of low alcohol and pure water; preferably, the mass fraction of the solution of low alcohol and pure water is 20%-50%. Preferably, the low alcohol has less than 4 carbon atoms.

[0019] As a preferred embodiment, the low alcohol is selected from one or a combination of 1,2-propanediol, 1,4-butanediol, 1,3-butanediol, 1,3-propanediol, glycerol.

[0020] As a preferred embodiment, the feeding speed in the cell wall breaking and extraction in step S1 is 15-25 L / min, and the extraction time is 15-35 min.

[0021] As a preferred embodiment, the centrifugal speed in the centrifugation in step S2 is 3000-8000 rpm, and the centrifugation time is 20-40 min.

[0022] As a preferred embodiment, the purification in step S2 is ceramic membrane separation purification, and the pore size is 300-1000 nm.

[0023] As a preferred embodiment, the concentration in step S2 is reverse osmosis membrane concentration, and the pore size is 0.1-10 nm; the concentration is to a feed liquid ratio of 1:(1-3).

[0024] The third aspect of the present application provides an application of a plant extract for reducing oil secretion, which is applied in skin care products and color cosmetics.

[0025] Compared with the prior art, the present application has the following beneficial effects:

[0026] (1) The plant extract for reducing oil secretion of the present application can inhibit 5 alpha reductase activity, inhibit sebaceous gland cell activity, and reduce skin oil secretion by the combined action of grapefruit and sophora, and dandelion.

[0027] (2) The plant extract for reducing oil secretion of the present application has good antioxidant effect by the combined action of sargassum and green tea, can alleviate the irritation of peroxide squalene to the skin caused by oxidation of sebum, and avoid inflammation; at the same time, the moisturizing component contained can reduce the loss of skin moisture, avoid excessive secretion of oil caused by dry skin, and solve the problems that the existing oil control skin care and color cosmetic products cannot fundamentally solve oil secretion, have obvious side effects, and have insufficient effect and easy recurrence.

[0028] (3) The plant extract for reducing oil secretion of the present application can be applied in various skin care or color cosmetic products, has high stability when directly applied in the products, and is not easy to deteriorate and fail. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 The test chart for moisturizing effect is shown in the figure;

[0030] Figure 2A comparison chart of 5α-reductase content;

[0031] Figure 3 Microscopic oil red staining results of lipid droplets for the blank control group;

[0032] Figure 4 Microscopic oil red staining results of lipid droplets for the negative control group;

[0033] Figure 5 Microscopic oil red staining results of lipid droplets for Example 2 (from left to right, parallel experiments);

[0034] Figure 6 A lipid droplet synthesis difference analysis chart for the blank control group, the negative control group, and the sample group (Example 2).

[0035] Figure 7 A stability test appearance chart of a plant extract for slowing down oil secretion applied to toner (from left to right, 2℃, 25℃, 45℃, 60℃).

[0036] Figure 8 A stability test appearance chart of a plant extract for slowing down oil secretion applied to cream (from left to right, -16℃, 2℃, 25℃, 50℃).

[0037] Figure 9 A stability test appearance chart of a plant extract for slowing down oil secretion applied to foundation (from left to right, -16℃, 2℃, 25℃, 50℃). DETAILED DESCRIPTION

[0038] Example 1

[0039] A plant extract for slowing down oil secretion, the plant raw materials include, by weight, 25 parts of citrus fruit, 4 parts of Sophora plant, 4 parts of chrysanthemum plant, 3 parts of seaweed plant, and 2 parts of Camellia plant.

[0040] The citrus fruit is grapefruit. The chrysanthemum plant is dandelion. The Sophora plant is Sophora flavescens. The seaweed plant is Sargassum thunbergii. The Camellia plant is green tea.

[0041] A preparation method of a plant extract for slowing down oil secretion, comprising the following steps:

[0042] S1 mixing and weighing the citrus fruit, Sophora plant, chrysanthemum plant, seaweed plant, and Camellia plant, crushing, adding solvent, and performing temperature-controlled directional wall-breaking extraction to obtain plant crude extract;

[0043] S2 sequentially performing centrifugation, purification, and concentration on the plant crude extract to obtain the plant extract.

[0044] The ratio of the plant raw material to the solvent is 1:15; the extraction temperature during the wall breaking extraction is 40°C.

[0045] The solvent is pure water.

[0046] The feeding speed during the wall breaking extraction in the step S1 is 15 L / min, and the extraction time is 25 min.

[0047] The centrifugal speed during the centrifugation in the step S2 is 3000 rpm, and the centrifugation time is 30 min.

[0048] The purification in the step S2 is ceramic membrane separation purification, and the pore size is 300 nm.

[0049] The concentration in the step S2 is reverse osmosis membrane concentration, the pore size is 1 nm, and the concentration is to a ratio of 1:2.

[0050] Example 2

[0051] A plant extract for reducing oil secretion, the plant raw material includes, by weight, 15 parts of citrus fruit, 6 parts of Sophora plant, 6 parts of chrysanthemum plant, 6 parts of seaweed plant, and 5 parts of Camellia plant.

[0052] The citrus fruit is grapefruit fruit. The chrysanthemum plant is dandelion. The Sophora plant is Sophora flavescens; the seaweed plant is Sargassum thunbergii; and the Camellia plant is green tea.

[0053] A preparation method of a plant extract for reducing oil secretion, comprising the following steps:

[0054] S1 mixing and weighing the citrus fruit, Sophora plant, chrysanthemum plant, seaweed plant, and Camellia plant, crushing them, adding a solvent, and performing temperature-controlled directional wall breaking extraction to obtain a plant crude extract;

[0055] S2 sequentially performing centrifugation, purification, and concentration on the plant crude extract to obtain a plant extract.

[0056] The ratio of the plant raw material to the solvent is 1:30; the extraction temperature during the wall breaking extraction is 25°C.

[0057] The solvent is a solution of butanediol and pure water with a mass fraction of 50%.

[0058] The feeding speed during the wall breaking in the step S1 is 20 L / min, and the extraction time is 30 min.

[0059] The centrifugal speed during the centrifugation in the step S2 is 4000 rpm, and the centrifugation time is 30 min.

[0060] The purification in the step S2 is ceramic membrane separation purification, and the pore size is 800 nm.

[0061] The concentration in step S2 is reverse osmosis membrane concentration, the pore size is 0.5 nm, and the concentration is to a feed liquid ratio of 1:2.

[0062] Example 3

[0063] A plant extract for reducing oil secretion, the plant raw materials include, by weight parts, 10 parts of citrus fruit, 5 parts of Sophora plant, 10 parts of chrysanthemum plant, 10 parts of seaweed plant, and 3 parts of Camellia plant.

[0064] The citrus fruit is grapefruit fruit. The chrysanthemum plant is dandelion. The Sophora plant is Sophora flavescens; the seaweed plant is Sargassum thunbergii; and the Camellia plant is green tea.

[0065] A preparation method of a plant extract for reducing oil secretion, comprising the following steps:

[0066] S1: mixing, weighing, and crushing the citrus fruit, Sophora plant, chrysanthemum plant, seaweed plant, and Camellia plant, adding a solvent, and performing temperature-controlled directional wall-breaking extraction to obtain a plant crude extract;

[0067] S2: sequentially performing centrifugation, purification, and concentration on the plant crude extract to obtain the plant extract.

[0068] The feed liquid ratio of the plant raw materials to the solvent is 1:40; and the extraction temperature during the wall-breaking extraction is 15℃.

[0069] The solvent is a solution of 1,3-propanediol and pure water, and the mass fraction is 50%.

[0070] The feeding speed during the wall-breaking extraction in step S1 is 25 L / min, and the extraction time is 15 min.

[0071] The centrifugation speed during the centrifugation in step S2 is 4000 rpm, and the centrifugation time is 30 min.

[0072] The purification in step S2 is ceramic membrane separation and purification, and the pore size is 600 nm.

[0073] The concentration in step S2 is reverse osmosis membrane concentration, the pore size is 0.1 nm, and the concentration is to a feed liquid ratio of 1:2.

[0074] Example 4

[0075] A plant extract for reducing oil secretion, the plant raw materials include, by weight parts, 8 parts of citrus fruit, 9 parts of Sophora plant, 10 parts of chrysanthemum plant, 5 parts of seaweed plant, and 6 parts of Camellia plant.

[0076] The citrus fruit is a grapefruit fruit. The compositae plant is a dandelion. The sophora plant is sophora flavescens; the seaweed plant is sargassum; and the camellia plant is green tea.

[0077] A method for preparing a plant extract for reducing oil secretion, comprising the following steps:

[0078] S1. Citrus fruits, sophora plants, compositae plants, seaweed plants, and camellia plants are mixed, weighed, crushed, added with a solvent, and subjected to temperature-controlled directional wall-breaking extraction to obtain a plant crude extract;

[0079] S2. The plant crude extract is subjected to centrifugation, purification, and concentration in sequence to obtain a plant extract.

[0080] The ratio of the plant raw material to the solvent is 1:35; and the extraction temperature during the wall-breaking extraction is 35°C.

[0081] The solvent is a solution of 1,3-propanediol and pure water with a mass fraction of 20%.

[0082] The feeding speed during the wall-breaking in step S1 is 20 L / min, and the extraction time is 35 min.

[0083] The centrifugation speed in step S2 is 8000 rpm, and the centrifugation time is 30 min.

[0084] The purification in step S2 is ceramic membrane separation purification, and the pore size is 1000 nm.

[0085] The concentration in step S2 is reverse osmosis membrane concentration, and the pore size is 10 nm. The concentration is performed until the ratio of the plant raw material to the solvent is 1:2.

[0086] Comparative Example 1

[0087] A plant extract for reducing oil secretion, wherein the plant raw material includes sophora plants 10 parts, compositae plants 10 parts, seaweed plants 10 parts, and camellia plants 8 parts by weight. Other specific embodiments are the same as those in Example 1.

[0088] Comparative Example 2

[0089] A plant extract for reducing oil secretion, wherein the plant raw material includes citrus fruits 26 parts, seaweed plants 6 parts, and camellia plants 6 parts by weight. Other specific embodiments are the same as those in Example 1.

[0090] Comparative Example 3

[0091] A plant extract for reducing oil secretion, wherein the plant raw material includes citrus fruits 26 parts, sophora plants 6 parts, and compositae plants 6 parts by weight. Other specific embodiments are the same as those in Example 1.

[0092] Comparative Example 4

[0093] A plant extract for alleviating sebum secretion, the plant raw materials comprising 6 parts of citrus, 4 parts of Sophora, 4 parts of chrysanthemum, 4 parts of seaweed, and 20 parts of Camellia, by weight. Other embodiments are the same as in Example 1.

[0094] Comparative Example 5

[0095] A plant extract for alleviating sebum secretion, the plant raw materials comprising 25 parts of Hamamelis, 4 parts of Sophora, 4 parts of chrysanthemum, 3 parts of seaweed, and 2 parts of Camellia, by weight. Other embodiments are the same as in Example 1.

[0096] Performance test

[0097] 1. DPPH radical scavenging experiment

[0098] 1.1 Test principle

[0099] DPPH is a stable free radical in organic solvents, its alcohol solution is purple, and needs to be stored in low temperature and dark, has a single electron, so it can accept an electron or hydrogen ion, and has a maximum absorption at a wavelength of 517 nm. In the presence of a free radical scavenger, the single electron of DPPH is captured, the color becomes lighter, the absorbance at the maximum light absorption wavelength decreases, and the degree of decrease is linear. The decrease in absorbance level indicates an increase in antioxidant activity, thereby evaluating the antioxidant capacity of the test sample. The antioxidant capacity is expressed by the inhibition rate, and the greater the inhibition rate, the stronger the antioxidant activity.

[0100] 1.2 Preparation of DPPH reagent

[0101] 0.0100 g of DPPH powder (molecular weight about 394) was accurately weighed. It was dissolved in 95% ethanol to 250 mL, and the concentration of the DPPH reagent was 0.1 mmol / L.

[0102] 1.3 Preparation of test sample

[0103] The plant extract prepared in the examples and comparative examples was dissolved in 95 wt% aqueous ethanol to prepare a 1.0% test solution. A 1 mg / mL ethyl ether control solution of Vitamin C was also prepared in the same manner.

[0104] 1.4 Antioxidant capacity test

[0105] a. In a 10 mL test tube, 4.0 mL of DPPH solution and 1.0 mL of 95 wt% ethanol were added in turn, mixed and shaken, and reacted in the dark for 30 min. After stabilization, the absorbance was measured at 517 nm with 95 wt% ethanol as a reference, and recorded as A0.

[0106] b. In a 10 mL test tube, add 4.0 mL DPPH solution and 1.0 mL sample solution to be tested, mix well, and react in the dark for 30 min. After stabilization, measure the absorbance at 517 nm with 95 wt% ethanol as the reference, and record the value as Ar.

[0107] c. In a 10 mL test tube, add 4.0 mL 95 wt% ethanol solution and 1.0 mL sample solution to be tested, mix well, and react in the dark for 30 min. After stabilization, measure the absorbance at 517 nm with 95 wt% ethanol as the reference, and record the value as As.

[0108] d. Calculation formula: DPPH free radical scavenging rate of sample:

[0109]

[0110] 1.5 Test results

[0111] The test results are shown in the DPPH free radical inhibition effect. When the addition amount of the embodiments 1-4 of the present application is 1 wt%, it has an anti-free radical effect equivalent to 1 mg / mL ethyl ether of vitamin C. The test results are shown in Table 1.

[0112] 2, ABTS free radical scavenging capacity test

[0113] 2.1 Test principle: ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) aqueous free radical initiator is used as a color developing agent. After ABTS is oxidized by active oxygen, a stable blue-green cationic free radical ABTS+· is generated. If there is an antioxidant component in the measured substance, the substance will react with ABTS+· to cause the reaction system to fade, and the change in absorbance (A734) at 734 nm is detected after 6 min.

[0114] 2.2 Preparation of reagents

[0115] a. PBS solution: weigh 8 g of NaCl, 0.2 g of KCl, 1.44 g of Na2HPO4, and 0.24 g of KH2PO4, adjust the pH to 7.2, and dissolve to 1000 mL.

[0116] b. ABTS+·: 2.45 mmol / L potassium persulfate was prepared, and ABTS was dissolved in potassium persulfate to prepare 7 mmoL / L ABTS stock solution, which was placed at room temperature and in the dark for 12-16 h. The stock solution was stable for 3-4 d. c. 2.45 mmol / L potassium persulfate: 100 mL was prepared, and 0.0662 g of potassium persulfate was dissolved in water to make up to 100 mL. d. 7 mmoL / L ABTS stock solution: 0.0384 g of ABTS was dissolved in 2.45 mmol / L potassium persulfate to make up to 10 mL, and the solution was dark green.

[0117] e. Preparation of ABTS+· assay solution: the ABTS+· stock solution was diluted with phosphate buffer (pH = 7.2) to make the absorbance at 734 nm wavelength reach 0.700 ± 0.020. When about 0.12 mmol / L of ABTS+was prepared, the measured absorbance was 0.716A (4 mL of ABTS+was prepared in 1 mL of PBS) - about 1.7 mL of mmoL / L ABTS stock solution was diluted to 100 mL.

[0118] 2.3 Test method

[0119] a. 4 mL of ABTS+· working solution was taken → 1 mL of sample was mixed for 10 s → 25°C was placed in the dark for 6 min → the absorbance was measured at 734 nm. 4 mL of PBS + 1 mL of sample was used as a reference, and A sample was recorded

[0120] b. 4 mL of ABTS+· working solution was taken → 1 mL of PBS was mixed for 10 s → 25°C was placed in the dark for 6 min → the absorbance was measured at 734 nm. PBS was used as a reference, and A0was recorded

[0121] Inhibition rate = (A0-A sample) / A0× 100%

[0122] 2.4 Test results

[0123] The test results are shown in Table 1. The total in vitro anti-free radical effect of the application examples 1-4 is equivalent to that of 0.1 mg / mL ethyl ether of vitamin C when the addition amount is 0.05 wt%.

[0124] 3. Hydroxyl radical scavenging rate by methyl violet method

[0125] 3.1 Test principle

[0126] This experiment was carried out in the presence of Fe 2+The net reaction of the catalysis of Fe2+ on H2O2 to generate H2O and O2. The intermediate product ·OH is generated during the reaction. ·OH selectively attacks the high electron cloud density points of methyl violet such as-C=C- groups due to its extremely high electronegativity (Ep=2.80V), and an electrophilic addition reaction occurs, resulting in the fading of methyl violet. In acidic conditions, Fe 2+ mainly exists in the form of Fe(OH)2, which can more quickly catalyze the generation of ·OH by hydrogen peroxide. The amount of ·OH generated can be determined by measuring the change in the absorbance value of methyl violet in the reaction system.

[0127] 3.2 Reagent configuration

[0128] a. Ferrous sulfate solution

[0129] Weigh 0.2487g of ferrous sulfate heptahydrate into dilute sulfuric acid and dilute to 100mL.

[0130] b. Hydrogen peroxide solution

[0131] Measure 0.088mL of hydrogen peroxide into water and dilute to 100mL.

[0132] c. Na2HPO4·citric acid buffer

[0133] Weigh 3.516g of sodium phosphate dibasic into water and dilute to 100mL; weigh 2.101g of citric acid monohydrate into water and dilute to 100mL; mix 8.82mL of Na2HPO4 with 11.18mL of citric acid and adjust the pH to 4.5.

[0134] d. Methyl violet solution

[0135] Weigh 0.1008g of methyl violet into water and dilute to 100mL

[0136] 3.3 Test method

[0137] a. The total reaction system is 5mL

[0138] b. Precisely weigh 1mL of methyl violet solution, 1mL of buffer, 1mL of sample solution, 1mL of ferrous sulfate solution, and finally add 1mL of H2O2, mix well, and mark as A S

[0139] c. Precisely weigh 1mL of methyl violet solution, 1mL of buffer, 1mL of distilled water, 1mL of ferrous sulfate solution, and finally add 1mL of H2O2, mix well, and mark as A

[0140] d. Precisely weigh 1mL of methyl violet solution, 1mL of buffer, 3mL of distilled water, mix well, and mark as A0

[0141] e. After mixing, the mixture was left at room temperature for 5 minutes, and then the absorbance change value ΔA of each system was measured on a spectrophotometer at an absorption wavelength of 582 nm with a 1 cm cuvette, and the clearance rate CR% was calculated

[0142] Calculation formula:

[0143] 3.4 Test results

[0144] The test results, as shown in the hydroxyl radical scavenging effect, are that the addition amount of the examples 1-4 of the present application is 1%, which is equivalent to the hydroxyl radical scavenging effect of 0.1 mg / mL ethyl ether of vitamin C. The test results are shown in Table 1.

[0145] 4. Moisturizing efficacy test

[0146] 4.1. Verification basis

[0147] GDCTC / OT-L012 "Moisturizing Efficacy (Physical Method) Test Procedure" (Method 1)

[0148] 4.2. Test method

[0149] 4.2.1 Before testing, place a saturated potassium carbonate solution at the bottom of the desiccator, so that the liquid depth is greater than 1 cm, place a porous partition, cover the lid, and keep it sealed. After the air vapor pressure is constant, the relative humidity can be maintained at 40% to 50%, and the test environment is 20°C to 25°C.

[0150] 4.2.2 Precisely weigh 2 g (accurate to 0.0001 g) of the sample (m0) into a constant weight weighing dish, and place it in the desiccator. After n hours (n is generally 2, 4, 8, 24 hours, and the actual test time is arranged according to the needs), take it out and weigh it (m n ). The sample is a plant extract prepared by the examples and the comparative examples.

[0151] 4.2.3 Prepare a positive control aqueous solution of a certain concentration, and test 2 g (accurate to 0.0001 g) of the positive control (10 wt% glycerol) aqueous solution according to the test method of 4.2.2, with three parallel tests, take the average value, and calculate the moisturizing rate of the sample after n hours.

[0152] 4.2.4 Judgment criteria

[0153] a. If the test sample moisturizing rate curve is better than that of the positive control, it means that the sample has a stronger moisturizing effect than the positive control;

[0154] b. If the test sample moisturizing rate curve is worse than that of the positive control, it means that the sample has a weaker moisturizing effect than the positive control;

[0155] c. If the test sample's moisture retention rate curve is better than that of the positive control at a certain time point, and worse than that of the positive control at a certain time point, it means that the sample has a stronger moisturizing effect than the positive control before a certain time point, and a weaker moisturizing effect than the positive control after a certain time point.

[0156] 4.3. Test results The moisturizing efficacy of the test results is shown in the following table: Figure 1 As shown in the table, Examples 1-4 have a stronger moisturizing rate than the positive control group, indicating that Examples 1-4 have a moisturizing effect under the experimental conditions. The test results of the moisturizing rate are shown in Table 2.

[0157] 5. Oil control effect test

[0158] 5.1.5 5α-reductase inhibition test method

[0159] 5.1.1 Test principle

[0160] Studies have shown that the main reason for excessive oil secretion is the induction of sebaceous gland lipid secretion by androgens. 5α-reductase (5α-R) is an important androgen metabolic enzyme in the skin, which can irreversibly convert testosterone (T) into dihydrotestosterone (DHT). DHT is the strongest androgen that can induce excessive secretion of sebaceous gland lipids. Reducing DHT levels by inhibiting 5α-R is a way to alleviate excessive oil secretion.

[0161] In this test, the 5α-R inhibition rate of the sample was determined according to the detection method of laboratory method QTTZ-XZ-39 "Verification and detection of oil control efficacy of cosmetics". By comparing with the impression control group, it is determined whether the sample has oil control efficacy.

[0162] 5.1.2 Test steps

[0163] a. Accurately weigh the sample and dilute it to a 0.5wt% aqueous solution;

[0164] b. Pretreated sample and 5α-reductase solution were reacted, and negative control and blank control were set up at the same time;

[0165] c. Measure OD value according to the operation of the detection kit;

[0166] d. Calculate the content of 5α-reductase according to the standard curve;

[0167] e. Calculate the 5α-reductase inhibition rate.

[0168] Calculation formula: Inhibition rate (%) = (1-T / C) x 100

[0169] T - 5α-reductase content of sample solution

[0170] C - 5α-reductase content of negative control

[0171] 5.1.3 Test results

[0172] Test results inhibition rate significance analysis table see Table 3, 5α-reductase content comparison chart see Figure 2 , the 5α-reductase inhibition rate of Example 2 is 19.15%, which indicates that the enzyme content of the plant formula extract prepared in Example 2 is significantly lower than the negative control. After SPSS software analysis, a two-tailed test is adopted, and the significance factor P < 0.05, indicating that the plant formula extract prepared in Example 2 has oil control effect.

[0173] 5.2. Sebaceous gland cell method

[0174] 5.2.1 Test principle

[0175] Based on human immortalized sebaceous gland cells SZ95, an in vitro oil secretion model is established by stimulating with hormone substances, and after drug treatment, the oil control effect of the test sample is evaluated by evaluating the trend of lipid droplet synthesis.

[0176] 5.2.2 Test method

[0177] a. Cell inoculation: dilute the cells to the inoculation density with the cell culture medium, inoculate into a 96-well plate, and the liquid volume per well is 200 μL. After inoculation, place it in a CO2 incubator for incubation for 24 h ± 2 h.

[0178] b. Drug treatment: after the cells in the 96-well plate reach the appropriate fusion degree, perform drug operation according to the following table experimental grouping. Discard the culture medium in the 96-well plate, add the culture medium containing the test substance to the test substance well; add normal cell culture medium to the blank control well, and the liquid volume per well is 200 μL. After drug administration, place the 96-well plate in a CO2 incubator for incubation for 24 h ± 2 h.

[0179] c. Staining: discard the old liquid in the 96-well plate, rinse the plate twice with buffer solution, add 100 μL of fixing solution per well to fix the cells, discard the fixing solution, add oil red solution 37°C for 30 min, then discard the oil red solution, and wash with appropriate ultrapure water for 5 min, repeat 2-3 times.

[0180] d. Photographing observation: take pictures under a microscope and record the staining results, and calculate the staining area ratio "%Area" using image analysis software.

[0181] e.Result analysis: SPSS analysis software was used, independent sample t-test was used for data analysis and statistics between groups, P<0.05 indicates statistical difference, represented by "*"; P>0.05 indicates no statistical difference.

[0182] Experimental grouping

[0183]

[0184] 5.2.3 Test results

[0185] a. Compared with the blank group, the lipid droplet synthesis of the negative control group increased significantly, indicating that the induction agent in this experiment was effective.

[0186] b. When the sample group was administered at a concentration of 0.5% (v / v), the lipid droplet synthesis of the test sample group decreased compared with the negative control group, with statistical difference (P<0.05), indicating that the test sample had the ability to inhibit lipid droplet synthesis at this concentration, which could be one of the evidences supporting the claim that the sample had oil control efficacy.

[0187] Figure 3 The results of the microscopic staining of lipid droplets with oil red for the blank control, Figure 4 The results of the microscopic staining of lipid droplets with oil red for the negative control, Figure 5 The results of the microscopic staining of lipid droplets with oil red for Example 2 (from left to right are parallel experiments). Figure 6 The difference analysis chart of lipid droplet synthesis for the blank control group, negative control group, and sample group (Example 2).

[0188] Table 1

[0189]

[0190]

[0191] Table 2

[0192] 2h 4h 8h 24h Positive control (10% glycerin) moisture retention (%) 97.6 95.3 93.2 80.7 Example 1 (1%) moisture retention (%) 97.7 95.2 93.2 82.4 Example 2 (1%) moisture retention (%) 97.7 95.4 93.3 82.9 Example 3 (1%) moisture retention (%) 98.4 95.4 93.1 82.7 Example 4 (1%) moisture retention (%) 97.8 95.3 93.2 82.4 Comparative Example 1 (1%) moisture retention (%) 97.3 95.1 92.4 80.1 Comparative Example 2 (1%) moisture retention (%) 95.7 92.5 88.4 76.5 Comparative Example 3 (1%) moisture retention (%) 92.2 89.7 83.1 70.4 Comparative Example 4 (1%) moisture retention (%) 97.5 93.2 89.4 79.8 Comparative Example 5 (1%) moisture retention (%) 94.4 90.7 86.9 77.4

[0193] Table 3

[0194]

[0195] 6. Application of a plant extract for reducing oil secretion in a toner, the preparation raw materials are as shown in Table 4,

[0196] Table 4

[0197] Serial number Raw material name Mass fraction % 1 Water To 100 2 Sodium hyaluronate 120W 0.02 3 Bentonite gel 1 4 Trehalose 0.5 5 EDTA-2Na 0.02 6 Nipagin methyl ester 0.1 7 Antimicro 9010 0.5 8 Butylene glycol 2 9 Example 2 2

[0198] The preparation method steps of a plant extract for reducing oil secretion in a toner are as follows:

[0199] (1) Sodium hyaluronate was added to butanediol for pre-dispersion;

[0200] (2) Mix items 1-8 together and stir to dissolve at 60℃;

[0201] (3) Cool down to 45℃, add item 9 and stir to restore to room temperature.

[0202] like Figure 7 As shown, in the stability test experiments at different temperatures of 2℃, 25℃, 45℃, and 60℃, the color change of the toner was not significant within 3 months, there was no obvious sedimentation, and the performance was stable (from left to right: 2℃, 25℃, 45℃, 60℃).

[0203] 7. Application of a plant extract that reduces sebum secretion in a face cream; the raw materials for preparation are shown in Table 5.

[0204] Table 5

[0205]

[0206]

[0207] The steps for preparing a face cream using a plant extract that reduces sebum secretion are as follows:

[0208] (1) Add each raw material in phase A into container 1, heat to 80°C, and stir until completely homogeneous;

[0209] (2) Add each raw material in phase B to container 2, heat to 80°C (sodium hyaluronate and xanthan gum are pre-dispersed with glycerol and butylene glycol), and stir until completely homogeneous;

[0210] (3) Add phase B to phase A and homogenize for 5 min;

[0211] (4) Add component C and stir for 30 minutes;

[0212] (5) Stir and cool down. After the product has cooled to 60°C, add phase D and stir for 5 minutes.

[0213] (6) Cool down to 45℃, add phase E and phase F, and stir for 10 min;

[0214] (7) Continue stirring and cool to room temperature.

[0215] like Figure 8As shown, in the stability test experiment at different temperatures of-16℃, 2℃, 25℃, 50℃, the color change of the cream within 3 months is not significant, there is no obvious precipitation, no emulsion separation phenomenon, and the performance is stable (from left to right, -16℃, 2℃, 25℃, 50℃).

[0216] 8. Use of a plant extract for reducing sebum secretion in the preparation of a foundation liquid, raw materials such as Table 6,

[0217] Table 6

[0218]

[0219]

[0220] A method for applying a plant extract for reducing sebum secretion in the preparation of a foundation liquid, the steps are as follows:

[0221] (1) Put phase A into container 1, heat to 80-85℃, dissolve completely;

[0222] (2) The temperature of the material in container 1 is lowered to 45℃, and phase B is added, stirred and dissolved uniformly, ready for use;

[0223] (3) Put phase B into container 2, mix and stir until the powder is fully immersed, then pass through a three-roll mill to disperse the color powder uniformly, and then heat to 80-85℃;

[0224] (4) After the temperature of the material in container 2 is raised, add phase D and dissolve completely;

[0225] (5) The temperature of the material in container 2 is lowered to 50℃, and the following phase E is added, stirred uniformly, and ready for use;

[0226] (6) Slowly pour the material in container 1 into container 2, and start stirring at the same time, until it is uniform. After the addition is complete, continue stirring for 10 minutes, homogenize at medium speed for 2 minutes, and then continue stirring for 10 minutes.

[0227] As Figure 9 shown, in the stability test experiment at different temperatures of-16℃, 2℃, 25℃, 50℃, the color change of the foundation liquid within 3 months is not significant, there is no obvious precipitation, no emulsion separation phenomenon, and the performance is stable (from left to right, -16℃, 2℃, 25℃, 50℃).

Claims

1. A plant extract that slows down sebum secretion, characterized in that, The plant raw material includes 5-25 parts of citrus fruit, 3-15 parts of Sophora plant, 3-15 parts of chrysanthemum plant, 3-15 parts of seaweed plant, and 1-10 parts of Camellia plant by weight; the preparation raw material further includes a solvent; The citrus fruit is grapefruit; the chrysanthemum plant is dandelion; the Sophora plant is Sophora flavescens; the seaweed plant is Sargassum macrophyllum; and the Camellia plant is green tea; The preparation method of the plant extract for slowing down secretion of oil and fat comprises the following steps: S1. Citrus fruit, Sophora plant, chrysanthemum plant, seaweed plant, and Camellia plant are mixed and weighed, pretreated, added with a solvent, and subjected to temperature-controlled directional wall-breaking extraction to obtain a plant crude extract; S2. The plant crude extract is sequentially subjected to centrifugation, purification, and concentration to obtain a plant extract; The solvent is selected from one or a combination of two of pure water and low alcohol.

2. A method for preparing a plant extract for reducing sebum excretion according to claim 1, characterized by, The method comprises the following steps: S1. Citrus fruit, Sophora plant, chrysanthemum plant, seaweed plant, and Camellia plant are mixed and weighed, pretreated, added with a solvent, and subjected to temperature-controlled directional wall-breaking extraction to obtain a plant crude extract; S2. The plant crude extract is sequentially subjected to centrifugation, purification, and concentration to obtain a plant extract.

3. The method of claim 2, wherein the plant extract is prepared by the steps of: (a) extracting a plant material with a solvent; (b) removing the solvent from the extract; (c) drying the extract; and (d) pulverizing the dried extract. The ratio of the plant raw material to the solvent is 1: (15-40); and the extraction temperature during the wall-breaking extraction is 15-40℃.

4. The method of claim 2, wherein the plant extract is prepared by the steps of: (a) extracting a plant material with a solvent; (b) removing the solvent from the extract; (c) drying the extract; and (d) pulverizing the dried extract. The feeding speed during the wall-breaking extraction in step S1 is 15-25 L / min, and the extraction time is 15-35 min.

5. The method of claim 2, wherein the plant extract is prepared by the steps of: (a) extracting a plant material with a solvent; (b) removing the solvent from the extract; (c) drying the extract; and (d) pulverizing the dried extract. The centrifugation speed during the centrifugation in step S2 is 3000-8000 rpm, and the centrifugation time is 20-40 min.

6. The method of claim 2, wherein the plant extract for reducing sebum secretion is prepared by the steps of: (a) extracting a plant material with a solvent; (b) removing the solvent from the extract; (c) drying the extract; and (d) pulverizing the dried extract. The purification in step S2 is ceramic membrane separation purification, and the pore size is 300-1000 nm.

7. The method of claim 2, wherein the plant extract is prepared by the steps of: a) extracting a plant material with a solvent; b) removing the solvent from the extract; c) drying the extract; and d) pulverizing the dried extract. The concentration in step S2 is reverse osmosis membrane concentration, and the pore size is 0.1-10 nm.

8. Use of a plant extract according to claim 1 for reducing the secretion of oil and fat, characterized in that, The plant extract is applied to the preparation of skin care products and color cosmetic products.

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