An eye drop and a method for preparing the same

Abstract

The application provides an eye drop and a preparation method thereof, the eye drop comprising an antibacterial agent, the antibacterial agent being composed of polyhexamethylene biguanide, taurine and extract of semen cassiae; the content of chrysophanol in the extract of semen cassiae is not less than 0.5%. The polyhexamethylene biguanide, taurine and extract of semen cassiae are matched to play a synergistic effect, so that the eye drop with better bactericidal effect is obtained.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of health care, in particular to an eye moistening liquid and a preparation method thereof. BACKGROUND

[0002] Nowadays, we face computers and mobile phones every day, and children read and do homework for a long time every day, so the eyes will have symptoms of dryness and roughness. At this time, many people will think of using eye moistening liquid to relieve eye fatigue, and the eye moistening liquid has won the favor of more and more people with eye problems due to its unique effect and convenience.

[0003] At present, the eye moistening liquid commonly purchased by people includes eye fatigue relieving eye drops and artificial tears, and the common components of the eye fatigue relieving eye drops are sodium hyaluronate (sodium glass acid), various vitamins (A, B, E), taurine, borneol, peppermint and the like, which can be used to lubricate eyeballs to relieve visual fatigue, relieve dry eyes, reduce red blood and the like. The common components of the artificial tears are sodium hyaluronate (sodium glass acid), chondroitin sulfate, methyl cellulose, polyethylene glycol and the like, and some also add components similar to oil to simulate the components of tears. The components of the artificial tears are relatively simple, and are specially used for dry eyes, stinging, and relieving dry eye.

[0004] The above-mentioned eye moistening liquid pays more attention to the moisturizing effect and ignores the problem that the eyeball surface is easy to breed bacteria due to improper protection of the eyes, not washing hands and kneading the eyes and the like. SUMMARY

[0005] In view of the problems in the prior art, the present application provides an eye moistening liquid and a preparation method thereof.

[0006] The present application provides an eye moistening liquid, which comprises an antibacterial agent, and the antibacterial agent is composed of polyhexamethylene biguanide, taurine and semen cassiae extract; the content of chrysophanol in the semen cassiae extract is not less than 0.5%.

[0007] Polyhexamethylene biguanide is a high molecular polymer bactericidal disinfectant, and the methyl guanidine therein has high activity and is easy to be adsorbed by bacteria and viruses, thereby inhibiting the reproduction ability of bacteria and viruses. It has the excellent properties of broad-spectrum bactericidal, low effective concentration, fast action speed, stable property, easy to dissolve in water, can be used at room temperature, long-term bacteriostasis and the like.

[0008] Taurine, chemical name 2-aminoethanesulfonic acid, is an organic compound with chemical formula C2H7NO3S. Taurine has a variety of important physiological functions in the human body. For example, taurine can strengthen the liver and gallbladder, reduce the cholesterol content of the liver, reduce the formation of cholesterol stones, and reduce alanine aminotransferase. Taurine has antipyretic and anti-inflammatory effects, can lower body temperature, lower blood pressure, strengthen the heart and prevent arrhythmia, inhibit platelet coagulation, help lower blood lipid levels, maintain normal blood pressure, and prevent arteriosclerosis. Taurine can also improve visual function and enhance nerve conduction. Therefore, the existing eye drops also often contain taurine, but basically for the purpose of improving visual function, and cannot pay attention to its antibacterial effect.

[0009] Semen Cassiae extract is a Chinese herbal medicine extract extracted from the dried mature seeds of Cassia bean or small Cassia. It contains a variety of active ingredients, such as rheochrysidin, emodin, aloe emodin and other anthraquinone derivatives, and semen cassiae, new moon spore rose pigment, semen cassiae, semen cassiae lactone and other components. Semen Cassiae extract has a variety of effects and functions, mainly including clearing the liver and eyesight, lowering blood pressure and blood lipids, and moistening the intestines and defecation. Semen Cassiae extract also has a good antibacterial effect. In the prior art, Semen Cassiae extract is used with Agarwood extract, Honeysuckle extract and Mulberry leaf extract to make eye drops.

[0010] The present application researches and finds that the combination of polyhexamethylene biguanide, taurine and semen cassiae extract can play a synergistic effect and achieve better sterilization effect.

[0011] In some embodiments of the present application, the mass percentage of polyhexamethylene biguanide is 0.1-1%, the mass percentage of taurine is 0.1-1%, and the mass percentage of semen cassiae extract is 3-5%, based on the total mass of the eye drops.

[0012] In preferred embodiments of the present application, the mass percentage of polyhexamethylene biguanide is 0.2%, the mass percentage of taurine is 0.3%, and the mass percentage of semen cassiae extract is 4%, based on the total mass of the eye drops.

[0013] In some embodiments of the present application, the eye drops further comprise a moisturizing factor, an osmotic pressure regulating factor, a chelating factor, a preservative, a lubricant and a pH regulator.

[0014] The osmotic pressure regulating factor acts as an osmotic pressure regulator to regulate osmotic pressure. Sodium chloride is usually selected.

[0015] The moisturizing factor, i.e. moisturizing agent, has good lubricating properties and can keep the surface of the eyeball moist. In some embodiments of the present application, the moisturizing factor is selected from one or more of hyaluronic acid, sodium hyaluronate, glycerol and propylene glycol.

[0016] Hyaluronic acid, also known as hyaluronic acid, is an acidic mucopolysaccharide. Its chemical formula is (CHNO). n Hyaluronic acid is a glycosaminoglycan composed of D-glucuronic acid and N-acetylglucosamine. It is widely distributed throughout the human body, especially in connective tissue, epithelial tissue, and nerve tissue. As a natural biomolecule in the skin, it has strong hydrophilicity, improving skin nutrition and metabolism, making the skin soft, smooth, wrinkle-free, more elastic, and preventing aging. Hyaluronic acid is also an ideal natural moisturizing factor; its affinity for water in solution is approximately 500 to 1000 times its own weight, exhibiting excellent moisturizing properties. Sodium hyaluronate, also known as sodium hyaluronate, can treat dry eye syndrome and repair corneal and conjunctival epithelial damage.

[0017] Chelating factors, also known as chelating agents, can bind to calcium deposits in the eye to improve vision and alleviate symptoms such as eye discomfort. In some embodiments of the present invention, the chelating factor is ethylenediaminetetraacetic acid (EDTA).

[0018] Preservatives prevent product contamination after opening, thus serving a preservative function. In some embodiments of the present invention, sodium perborate is selected as the preservative.

[0019] The lubricant has a lubricating effect. In some embodiments of the present invention, the lubricant is one or more of borax, polyvinylpyrrolidone, and glycerin, which also has anti-corrosion properties and helps to relieve eye fatigue.

[0020] In some embodiments of the present invention, the eye drops further include active ingredients, including borneol. Borneol, due to its spicy and cooling properties, provides a cooling and antipruritic effect, and is effective in relieving dryness and soreness in the eyes.

[0021] In some embodiments of the present invention, the eye drops comprise, by weight percentage, the following components:

[0022] Polyhexamethylene biguanide 0.1-1%, taurine 0.1-1%, cassia seed extract 3-5%, sodium chloride 0.1-1.5%, ethylenediaminetetraacetic acid 0.01-0.1%, sodium hyaluronate 0.5-2%, borneol 1-3%, borax 0.1-1%, pH adjuster 1-3%, preservative 0.1-1%, and the balance being water.

[0023] It is understood that the water in the eye drops is sterile water that meets the requirements.

[0024] The eye drops prepared using the above-mentioned formula can not only effectively inhibit the growth of microorganisms in the eyes, but also improve the daily care of dry eye syndrome, relieve eye fatigue, and improve vision to a certain extent.

[0025] Further preferably, the eye drops comprise the following components in percentage by mass:

[0026] Polyhexamethylene biguanide 0.2%, taurine 0.3%, Cassia extract 4%, sodium chloride 1%, ethylenediaminetetraacetic acid 0.02%, sodium hyaluronate 0.8%, borneol 1.4%, borax 0.5%, sodium dihydrogen phosphate 1.2%, sodium perborate 0.3%, and the balance of water.

[0027] The application also provides a preparation method of the above-mentioned eye drops, comprising the following steps:

[0028] S1, weigh the borneol, add an appropriate amount of ethanol, and grind until completely dissolved, ready for use;

[0029] S2, divide the water into two parts, weigh the sodium chloride, sodium hyaluronate, Cassia extract and taurine into a sterilized container, add one part of water, stir and dissolve at 40±5℃, and prepare A liquid;

[0030] S3, weigh the borax, preservative, polyhexamethylene biguanide and ethylenediaminetetraacetic acid into a sterile container, and add the other part of water, heat in a water bath at 75±5℃, and prepare B liquid;

[0031] S4, mix the dissolved borneol with A liquid and B liquid to prepare C liquid, and cool to 25℃;

[0032] S5, add a pH adjuster to the C liquid to adjust the pH of the product to between 6.5 and 7.2;

[0033] S6, filter the above-mentioned solution through a 0.1-2 micron filter, sterilize and package, and the eye drops are obtained.

[0034] Further, the preparation method of the Cassia extract comprises: weighing Cassia powder, adding an ethanol and ethyl acetate mixed solution with a volume ratio of 2:1 at a solid-liquid ratio of 1:5, refluxing for 3 times, combining the extraction liquids, concentrating to a certain volume under reduced pressure, washing out with 60% ethanol, removing the solvent, and vacuum drying to obtain the Cassia extract.

[0035] The application provides an eye drop and a preparation method thereof, which has better bactericidal effect by the synergistic effect of polyhexamethylene biguanide, taurine and Cassia extract. DETAILED DESCRIPTION

[0036] In order to make the objects, technical solutions and advantages of the present application clearer, the technical solutions in the present application will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0037] If not specifically indicated, the technical means used in the embodiments of the present application are all conventional means known by those skilled in the art. If not specifically indicated, the materials and reagents used in the embodiments of the present application can be obtained through regular commercial channels.

[0038] In the following embodiments, the preparation method of the Cassia extract is as follows:

[0039] The required Cassia powder (passed through a No. 3 sieve) is weighed and accurately weighed, and is placed in a 250 mL round-bottom flask. A solution of a certain concentration of ethanol and ethyl acetate mixed at a ratio of 2:1 (mL:mL) is added at a liquid-to-solid ratio of 1:5. The extraction is performed by water bath reflux for 3 times, each time for a specified time. The filtrate is combined and concentrated under reduced pressure to a certain volume. The 60% ethanol is used to wash out, and the solvent is dried on a water bath. Vacuum drying (pressure-0.1 MPa, temperature 70°C, same below) is performed for 48 h to obtain the Cassia extract.

[0040] Embodiment 1

[0041] The present embodiment provides an eye drop, and the components are as follows in mass percentage:

[0042] Polyhexamethylene biguanide 0.2%, taurine 0.3%, Cassia extract 4%, sodium chloride 1%, ethylenediaminetetraacetic acid 0.02%, sodium hyaluronate 0.8%, borneol 1.4%, borax 0.5%, sodium dihydrogen phosphate 1.2%, sodium perborate 0.3%, and the balance of water.

[0043] The present embodiment also provides a preparation method of the above-mentioned eye drop, and the steps are as follows:

[0044] S1, borneol is weighed and added with an appropriate amount of ethanol, and is ground until completely dissolved, and is used as needed;

[0045] S2, water is divided into two parts, and sodium chloride, sodium hyaluronate, Cassia extract and taurine are weighed into a sterilized container, and one part of water is added, and is stirred and dissolved at 40°C to prepare A liquid;

[0046] S3, borax, sodium perborate, polyhexamethylene biguanide and ethylenediaminetetraacetic acid are weighed into a sterile container, and the other part of water is added, and is heated in a water bath at 75°C to prepare B liquid;

[0047] S4, the dissolved borneol is mixed with A liquid and B liquid to prepare C liquid, and is cooled to 25°C;

[0048] S5, adding sodium dihydrogen phosphate in the C solution, adjusting the pH of the product to 6.5-7.2;

[0049] S6, filtering the above obtained solution through a filter of 0.1-2 microns, and then sterilizing and packaging to obtain the eye drops.

[0050] Example 2

[0051] The present example provides an eye drop, the components of which are as follows in mass percentage:

[0052] Polyhexamethylene biguanide 1%, taurine 1%, cassia extract 5%, sodium chloride 1%, ethylenediaminetetraacetic acid 0.02%, sodium hyaluronate 0.8%, borneol 1.4%, borax 0.5%, sodium dihydrogen phosphate 1.2%, sodium perborate 0.3%, and the balance being water.

[0053] The present example also provides a preparation method of the above eye drop, the steps of which are as follows:

[0054] S1, weighing borneol, adding an appropriate amount of ethanol, and grinding until completely dissolved, for later use;

[0055] S2, dividing the water into two parts, weighing sodium chloride, sodium hyaluronate, cassia extract, and taurine into a sterilized container, adding one part of the water, and stirring to dissolve at 40°C to prepare A solution;

[0056] S3, weighing borax, sodium perborate, polyhexamethylene biguanide, and ethylenediaminetetraacetic acid into a sterile container, adding the other part of the water, and heating in a water bath at 75°C to prepare B solution;

[0057] S4, mixing the dissolved borneol with A solution and B solution to prepare C solution, and cooling to 25°C;

[0058] S5, adding sodium dihydrogen phosphate in the C solution, adjusting the pH of the product to 6.5-7.2;

[0059] S6, filtering the above obtained solution through a filter of 0.1-2 microns, and then sterilizing and packaging to obtain the eye drops.

[0060] Example 3

[0061] The present example provides an eye drop, the components of which are as follows in mass percentage:

[0062] Polyhexamethylene biguanide 0.5%, taurine 0.5%, cassia extract 4%, sodium chloride 1%, ethylenediaminetetraacetic acid 0.02%, sodium hyaluronate 2%, propyl alcohol 1%, borneol 3%, borax 0.5%, sodium dihydrogen phosphate 1.2%, sodium perborate 0.2%, and the balance being water.

[0063] The embodiment also provides a preparation method of the eye drops.

[0064] S1, weigh borneol, add an appropriate amount of ethanol, grind until completely dissolved, and wait for use;

[0065] S2, divide the water into two parts, weigh sodium chloride, sodium hyaluronate, cassia seed extract and taurine into a sterilized container, add one part of water, stir and dissolve at 40°C, and prepare A liquid;

[0066] S3, weigh borax, sodium perborate, polyhexamethylene biguanide and ethylenediaminetetraacetic acid into a sterile container, add the other part of water, heat in a water bath at 75°C, and prepare B liquid;

[0067] S4, mix the dissolved borneol with A liquid and B liquid to prepare C liquid, and cool to 25°C;

[0068] S5, add sodium dihydrogen phosphate to the C liquid to adjust the pH of the product to 6.5-7.2;

[0069] S6, filter the resulting solution through a 0.1-2 micron filter, sterilize and package to obtain the eye drops.

[0070] Comparative Example 1

[0071] The comparative example provides an eye drop, the components of which are as follows in mass percentage:

[0072] Polyhexamethylene biguanide 4.5%, sodium chloride 1%, ethylenediaminetetraacetic acid 0.02%, sodium hyaluronate 0.8%, borneol 1.4%, borax 0.5%, sodium dihydrogen phosphate 1.2%, sodium perborate 0.3%, and the balance of water. The preparation method is the same as that of Example 1.

[0073] Comparative Example 2

[0074] The comparative example provides an eye drop, the components of which are as follows in mass percentage:

[0075] Taurine 4.5%, sodium chloride 1%, ethylenediaminetetraacetic acid 0.02%, sodium hyaluronate 0.8%, borneol 1.4%, borax 0.5%, sodium dihydrogen phosphate 1.2%, sodium perborate 0.3%, and the balance of water. The preparation method is the same as that of Example 1.

[0076] Comparative Example 3

[0077] The comparative example provides an eye drop, the components of which are as follows in mass percentage:

[0078] Cassia extract 4.5%, sodium chloride 1%, ethylenediaminetetraacetic acid 0.02%, sodium hyaluronate 0.8%, borneol 1.4%, borax 0.5%, sodium dihydrogen phosphate 1.2%, sodium perborate 0.3%, and the balance of water. The preparation method is the same as that of Example 1.

[0079] Comparative Example 4

[0080] The present comparative example provides an eye drop, the components of which are as follows in mass percentage:

[0081] Triclosan 0.5%, Cassia extract 4%, sodium chloride 1%, ethylenediaminetetraacetic acid 0.02%, sodium hyaluronate 0.8%, borneol 1.4%, borax 0.5%, sodium dihydrogen phosphate 1.2%, sodium perborate 0.3%, and the balance of water. The preparation method is the same as that of Example 1.

[0082] Comparative Example 5

[0083] The present comparative example provides an eye drop, the components of which are as follows in mass percentage:

[0084] Polyhexamethylene biguanide 0.2%, polyhexamethylene monoguanide 0.3%, Cassia extract 4%, sodium chloride 1%, ethylenediaminetetraacetic acid 0.02%, sodium hyaluronate 0.8%, borneol 1.4%, borax 0.5%, sodium dihydrogen phosphate 1.2%, sodium perborate 0.3%, and the balance of water. The relative molecular weight of the polyhexamethylene monoguanide is 1000 kDa-5000 kDa.

[0085] The preparation method is the same as that of Example 1.

[0086] Comparative Example 6

[0087] The present comparative example provides an eye drop, the components of which are as follows in mass percentage:

[0088] Compound eaglewood traditional Chinese medicine composition (eaglewood extract 20 parts, Cassia extract 1 part, honeysuckle extract 15 parts, and mulberry leaf extract 1 part) 30 parts, hyaluronic acid 10 parts, antioxidant 10 parts, phosphate buffer 10 parts.

[0089] The preparation method is as follows:

[0090] First, the above traditional Chinese medicine composition and hyaluronic acid are mixed, and then the antioxidant and phosphate buffer are added and fully mixed and stirred to uniformity. The mixed solution is then left to stand for 6 hours, filtered with a microporous filter membrane, and an eye drop is obtained.

[0091] Performance test

[0092] The eye drops obtained in the above examples and comparative examples are subjected to performance tests.

[0093] 1. Bacteriostatic test

[0094] The test method is QB / T 2738 antibacterial test method. The test results are shown in Table 1, wherein the antibacterial rate > 50%, indicating that the product has antibacterial effect, and the antibacterial rate > 90%, indicating that the product has strong antibacterial effect.

[0095] Table 1

[0096]

[0097] 2. Alleviating fatigue and improving eye dryness test

[0098] Recruit 20 people with eye problems, use the product of the application and other two certain marketed eye drops products for comparative test. Add eye drops at 9:30-11:30, 12:30-14:30 and 15:30-17:30, score the above effects, and collect data to calculate the average value. The results are shown in Table 2.

[0099] Table 2

[0100] Table 2

[0101]

[0102]

[0103] Note: The product of the application in the table is a mixture of products of Examples 1-3.

[0104] The above results show that the product of the application has obvious advantages over the marketed product in relieving eye dryness and relieving eye pain; and has performance comparable to the marketed product in relieving fatigue and improving eyesight and sleep.

[0105] It should be noted that the endpoints of the ranges and any values disclosed herein are not limited to the precise values or the exact range, and these ranges and values should be interpreted as approximately the values. For numerical ranges, the endpoints of each range, the endpoints of each range and individual point values, and individual point values can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed herein.

[0106] In the description of the specification, the description of the terms "one embodiment", "some embodiments", "specific embodiments", or "some specific embodiments" etc. means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are contained in at least one embodiment or example of the embodiments of the present application. In the specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the person skilled in the art can combine and combine the different embodiments or examples described in the specification and the features of the different embodiments or examples without contradiction.

[0107] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solution deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. An eye drop solution, characterized by, The eye drops consist of the following components in mass percentage: Polyhexamethylene biguanide 0.1-1%, taurine 0.1-1%, cassia extract 3-5%, sodium chloride 0.1-1.5%, ethylenediaminetetraacetic acid 0.01-0.1%, sodium hyaluronate 0.5-2%, borneol 1-3%, borax 0.1-1%, pH adjuster 1-3%, preservative 0.1-1%, and the balance of water.

2. The eye drops according to claim 1, characterized in that, The eye drops consist of the following components in mass percentage: Polyhexamethylene biguanide 0.2%, taurine 0.3%, cassia extract 4%, sodium chloride 1%, ethylenediaminetetraacetic acid 0.02%, sodium hyaluronate 0.8%, borneol 1.4%, borax 0.5%, sodium dihydrogen phosphate 1.2%, sodium perborate 0.3%, and the balance of water.

3. The method of preparing the eye drops as claimed in claim 1 or 2, characterized in that, The method comprises the following steps: S1, weigh the borneol, add an appropriate amount of ethanol, grind until completely dissolved, and wait for use; S2, divide the water into two parts, weigh the sodium chloride, sodium hyaluronate, cassia extract, and taurine into a sterilized container, add one part of water, stir and dissolve at 40±5℃, and prepare A liquid; S3, weigh the borax, preservative, polyhexamethylene biguanide, and ethylenediaminetetraacetic acid into a sterile container, add the other part of water, heat in a water bath at 75±5℃, and prepare B liquid; S4, mix the dissolved borneol with A liquid and B liquid to prepare C liquid, and cool to 25℃; S5, add the pH adjuster to the C liquid to adjust the product pH to 6.5~ 7.2; S6, filter the product obtained above through a 0.1-2 micron filter, sterilize and package, and obtain the eye drops.

4. The method of claim 3, wherein the eye drops are prepared by, The preparation method of the cassia extract is: Weigh the cassia powder, add a mixed solution of ethanol and ethyl acetate with a volume ratio of 2:1 at a solid-liquid ratio of 1:5, reflux extract 3 times, combine the extract, concentrate to a certain volume under reduced pressure, wash with 60% ethanol, remove the solvent, and vacuum dry to obtain the cassia extract.

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