A stem cell culture device

Abstract

The present invention provides a stem cell culture device, comprising a box, two rows of tube racks and a rack moving device. The box can be opened and closed, and has an upper space and a lower space therein. An observation window is provided on one side of the box. Two rows of tube racks are arranged up and down, respectively located in the upper space and the lower space. Multiple culture tubes can be placed horizontally on each tube rack. The tube racks in the upper space can be moved in sequence to approach the observation window. The rack moving device can vertically move the tube racks in the upper space close to the observation window to the lower space, and can vertically move the tube racks in the lower space away from the observation window to the upper space. The present invention moves the tube racks in the upper space toward the observation window in sequence, so that each tube rack can be moved to the observation window for observation, and then the tube racks that have been observed are moved to the lower space by the rack moving device and away from the observation window. In this cycle, the culture tubes on all tube racks can pass through the observation window for detailed observation.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture devices, and in particular to a stem cell culture device. Background Art

[0002] During the stem cell culture process, the state of the stem cells needs to be observed frequently. In order to prevent the incubator from opening and closing, and the outside air from entering the incubator and contaminating the stem cell culture, a transparent observation window is usually opened on the side wall of the incubator for observation. However, during large-scale stem cell culture, multiple containers containing stem cells are arranged together, and there is a blind spot when observing from one side of the observation window, making it impossible to observe the stem cells in all containers.

[0003] Patent CN116970487A discloses a neural stem cell culture device, the scheme of which is as follows: a placement rack is rotatably connected in an incubator, a plurality of placement holes are provided on the placement rack, a through hole is provided at the bottom of the incubator directly below any placement hole, a support bolt is threadedly connected to the through hole, and a rotating handle is rotatably and sealably connected to the incubator for driving the through hole and the culture tube to rotate relative to each other. The culture tubes in the placement holes on the placement rack are driven to pass through the observation window of the incubator in sequence by the rotation of the rotating handle. When a certain culture tube needs to be observed in detail, the support bolt in the through hole can be removed, and the culture tube slides downward from the through hole, which is convenient for real-time observation. However, on the one hand, multiple culture tubes are gathered together, and the culture tube in the middle is far away from the observation window, and is blocked by the culture tubes in the outer circle, making it difficult to observe; on the other hand, when multiple culture tubes need to be observed in detail, the support bolts at different positions need to be removed, which is inconvenient. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a stem cell culture device, which can solve or at least alleviate one or more of the above-mentioned problems and other problems in the prior art.

[0005] The present invention provides a stem cell culture device, comprising:

[0006] The box can be opened and closed, and has an upper space and a lower space therein, and an observation window is provided on one side thereof;

[0007] Two rows of tube racks are arranged in an upper and lower manner, respectively located in the upper space and the lower space, each of the tube racks can be horizontally placed with multiple culture tubes, the tube racks in the upper space can be moved successively closer to the observation window, and the tube racks in the lower space can be moved successively away from the observation window; and

[0008] The rack moving device can vertically move the pipe rack in the upper space close to the observation window to the lower space, and can vertically move the pipe rack in the lower space far from the observation window to the upper space.

[0009] Preferably, the housing comprises:

[0010] Two parallel side panels;

[0011] Two first strip-shaped guide blocks are parallel to each other, one end of which is close to the observation window and is tilted downward, and each is located on the inner side of the side plate on the same side; and

[0012] Two second strip guide blocks parallel to each other, with one end away from the observation window tilted downward, located below the first strip guide block, and each located on the inner side of the side plate on the same side;

[0013] The two ends of the tube placement rack can slide on the first strip guide block or the second strip guide block.

[0014] Preferably, the tube placement rack comprises:

[0015] Frame body;

[0016] Two coaxial first pulleys are rotatably connected to the upper parts of both ends of the frame body, respectively, and can fit with the second strip guide block on the same side; and

[0017] Two coaxial second pulleys are rotatably connected to the upper parts of both ends of the frame body respectively, are coaxial with the first pulley, and can fit with the first strip guide block on the same side.

[0018] Preferably, the rack moving device comprises two rack moving mechanisms parallel to each other, the two rack moving mechanisms are respectively located at two ends of the pipe placing rack, and the rack moving mechanisms comprise:

[0019] A downward moving member is disposed in the box body and can be moved vertically, and is located outside one end of the first strip guide block close to the observation window, and has a first guide portion disposed obliquely and a blocking portion connected to the lower end of the first guide portion;

[0020] An upward movement mechanism, capable of vertically moving the pipe placement rack on the second strip guide block away from the observation window to the first strip guide block;

[0021] A first limiting mechanism, capable of preventing the second pulley on the first strip guide block from being separated therefrom; and

[0022] A driving mechanism, used for driving the downward moving member and the upward moving mechanism;

[0023] The first guide portion is matched with the second pulley, the first guide portion can receive the second pulley from the lower end of the first strip guide block, and the blocking portion can prevent the second pulley from slipping off the first guide portion.

[0024] Preferably, the upward movement mechanism comprises:

[0025] A vertical block is vertically movably disposed in the box and is located outside an end of the first strip guide block away from the observation window;

[0026] A connecting block connected to the vertical block and extending toward the observation window;

[0027] a stopper connected to an end of the connecting block away from the vertical block;

[0028] A second guide rod, which is matched with the second pulley and can be connected to the first strip guide block, one end of which is rotatably connected to the lower end of the vertical block, and the other end of which can be rotated to be vertical and abut against the upper end of the vertical block, and the other end of which can also be rotated to be inclined and abut against the upper end of the stop block;

[0029] a torsion spring, arranged on the vertical block, for giving the end of the second guide rod away from the vertical block a tendency to approach the stop block; and

[0030] A second limiting mechanism, capable of preventing the first pulley on the second guide rod from slipping off;

[0031] The driving mechanism can drive the vertical block to move up and down.

[0032] Preferably, the rack moving mechanism further comprises a mounting plate connected to the inner wall of the box body, and the driving mechanism comprises:

[0033] A driving member, the two ends of which are respectively connected to the vertical block and the blocking portion, and a driving hole is formed on the driving member;

[0034] A motor, mounted on the mounting plate;

[0035] A screw rod has one end connected to the output end of the motor, and the other end passes through the driving hole and is threadedly connected to the driving hole.

[0036] Preferably, a mounting groove is provided on a side of the mounting plate facing the observation window;

[0037] The first limiting mechanism comprises:

[0038] A sliding block, slidably disposed in the mounting groove and capable of sliding along the length direction of the mounting groove;

[0039] A limit spring, arranged in the installation groove, with one end connected to the sliding block and the other end connected to the inner wall of the box body;

[0040] a limiting block connected to the sliding block, an end of which can be moved to the upper side of the lower end of the first strip guide block and form a block for the second pulley, and a limiting square hole vertically penetrating therethrough is provided on the limiting block; and

[0041] A trigger square rod, the lower end of which is connected to the drive, and the upper end of which is provided with a guiding inclined surface;

[0042] The guiding inclined surface may abut against an end portion of the limiting square hole away from the first strip-shaped guide block.

[0043] Preferably, the second limiting mechanism includes:

[0044] A strip-shaped anti-slip block, the upper end of which is connected to the mounting plate, the upper end of which is located between the end of the first strip-shaped guide block away from the observation window and the stopper, and the lower end of which is located above the second pulley in the lower space;

[0045] The strip-shaped anti-slip block can block the lifted second pulley.

[0046] Compared with the prior art, the present invention has the following beneficial effects:

[0047] In the technology of the present invention, the tube racks in the upper space are moved toward the observation window in sequence, so that each tube rack can be moved to the observation window for observation, and then the observed tube racks are moved to the lower space by the rack moving device and away from the observation window, and the cycle continues. No additional operation is required, and the culture tubes on all the tube racks can pass through the observation window for detailed observation. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for the specific embodiments or the description of the prior art. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn according to the actual scale.

[0049] Figure 1 is a three-dimensional diagram of a stem cell culture device in one embodiment of the present invention;

[0050] Figure 2 for Figure 1 Another stereoscopic view of (the top plate and a side plate are not shown);

[0051] Figure 3 for Figure 2 A top view of

[0052] Figure 4 for Figure 2 Another view of (without box);

[0053] Figure 5 for Figure 4 Another view of the device (only two tube racks are retained);

[0054] Figure 6 for Figure 5 A magnified image of point A;

[0055] Figure 7 for Figure 5 Enlarged view of point B (exploded view);

[0056] Figure 8 for Figure 7 Another view of .

[0057] Reference numerals:

[0058] 10. Box body; 11. Upper space; 12. Lower space; 13. Observation window; 14. Side panel; 15. First strip guide block; 16. Second strip guide block;

[0059] 20. tube rack; 21. culture tube; 22. rack body; 23. first pulley; 24. second pulley;

[0060] 30. Frame shifting mechanism; 31. Downward moving member; 311. First guide portion; 312. Blocking portion; 32. Upward moving mechanism; 321. Vertical block; 322. Connecting block; 323. Blocking block; 324. Second guide rod; 325. Torsion spring; 326. Strip anti-dropping block; 33. First limiting mechanism; 331. Sliding block; 332. Limiting spring; 333. Limiting block; 334. Triggering square rod; 335. Limiting square hole; 336. Guide slope; 34. Driving mechanism; 341. Driving member; 342. Motor; 343. Screw; 35. Mounting plate; 351. Mounting groove. DETAILED DESCRIPTION

[0061] The following embodiments of the technical solution of the present invention are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and are therefore only used as examples, and cannot be used to limit the protection scope of the present invention.

[0062] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0063] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0064] In addition, the terms "first", "second", etc. are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. In the description of the present invention, the meaning of "plurality" is more than two, unless otherwise clearly and specifically defined.

[0065] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0066] In the present application, unless otherwise clearly specified and limited, a first feature being “above” or “below” a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being “above”, “above”, and “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0067] See also Figures 1 to 8 This embodiment provides a stem cell culture device, including a box body 10, two rows of tube racks 20 and a rack moving device.

[0068] The box body 10 can be opened and closed, and has an upper space 11 and a lower space 12 therein. An observation window 13 is provided on one side thereof, and the observation window 13 corresponds to the upper space 11. The upper space 11 and the lower space 12 can be observed through the observation window 13. The box body 10 is sealed after being closed.

[0069] Two rows of tube racks 20 are arranged up and down, respectively located in the upper space 11 and the lower space 12. Multiple culture tubes 21 can be placed horizontally on each tube rack 20, and the tube racks 20 in the upper space 11 can be moved to approach the observation window 13 in sequence. When a tube rack 20 moves to the observation window 13, the multiple culture tubes 21 on the tube rack 20 are at the same distance from the observation window 13. The tube racks 20 in the lower space 12 can be moved away from the observation window 13 in sequence.

[0070] The rack moving device can vertically move the pipe rack 20 near the observation window 13 in the upper space 11 to the lower space 12, and can vertically move the pipe rack 20 far from the observation window 13 in the lower space 12 to the upper space 11. Figure 2 When the observation window 13 is on the right side, the rack moving device can make all the tube racks 20 move in a clockwise cycle.

[0071] In this embodiment, stem cells are cultured in the culture tube 21, and more than one culture tube 21 can be placed in the same tube rack 20. The tube racks 20 in the upper space 11 are moved toward the observation window 13 in sequence, so that each tube rack 20 can be moved to the observation window 13 for observation, and then the observed tube racks 20 are moved to the lower space 12 by the rack moving device and away from the observation window 13, and the cycle continues. No additional operation is required, and the culture tubes 21 on all tube racks 20 can pass through the observation window 13 for detailed observation.

[0072] In one embodiment, the box 10 includes two mutually parallel side panels 14, two mutually parallel first strip guide blocks 15 and two mutually parallel second strip guide blocks 16. In addition, the box 10 also includes a top panel and a front panel. The top panel can be opened and closed, and the culture tube 21 can be placed on the tube rack 20 after the top panel is opened. The observation window 13 is opened on the front panel, and the observation window 13 is sealed with glass material.

[0073] One end of the two first strip guide blocks 15 close to the observation window 13 is tilted downward, that is, the height of one end of the first strip guide block 15 close to the observation window 13 is lower than the other end thereof. The two first strip guide blocks 15 are respectively located on the inner side of the side plate 14 on the same side.

[0074] The ends of the two second strip guide blocks 16 away from the observation window 13 are tilted downward, that is, the height of the ends away from the observation window 13 is lower than the height of the other ends close to the observation window 13. The two second strip guide blocks 16 are both located below the first strip guide block 15, and are each located on the inner side of the side plate 14 on the same side. The two ends of the tube rack 20 can slide on the first strip guide block 15 or the second strip guide block 16. Specifically, the ends of the first strip guide block 15 and the second strip guide block 16 are each connected to the inner wall of the box body 10.

[0075] In this embodiment, the tube rack 20 can slide on the first strip guide block 15 and the second strip guide block 16, and the multiple tube racks 20 located on the first strip guide block 15 can slide along the first strip guide block 15 close to the observation window 13 under the action of gravity until the storage rack at the observation window 13 is moved to the second strip guide block 16 by the rack moving device.

[0076] In one embodiment, the tube placement rack 20 includes a rack body 22 , two coaxial first pulleys and two coaxial second pulleys 24 .

[0077] The two coaxial first pulleys 23 are respectively connected to the upper parts of the two ends of the frame body 22, and can fit with the second strip guide block 16 on the same side, that is, the second strip guide block 16 can be embedded in the groove along the circumferential direction of the first pulley 23 and maintain a gap therebetween. Therefore, the first pulley 23 can slide or roll on the second strip guide block 16.

[0078] Similarly, two coaxial second pulleys 24 are respectively connected to the upper parts of both ends of the frame body 22, coaxial with the first pulley 23, and can fit with the first strip guide block 15 on the same side. The second pulley 24 can slide or roll on the first strip guide block 15. Specifically, the first pulley 23 and the second pulley 24 are both rotatably connected to the frame body 22, and their rotation center lines coincide with their axis lines.

[0079] In addition, the frame body 22 includes a guide plate, a limit plate and a connecting piece.

[0080] The guide plate is provided with a plurality of guide holes arranged in a row for the culture tube 21 to slide through. The limiting plate is connected to the guide plate through a connecting piece, and the limiting plate is located below the guide plate. The limiting plate is provided with limiting grooves corresponding to the guide holes one by one. The limiting grooves can limit the lower end of the culture tube 21.

[0081] In this embodiment, the culture tube 21 is inserted from the guide hole on the guide plate and directly reaches the limiting groove on the limiting plate to be limited, thereby completing the installation of the culture tube 21. Since the first pulley 23 and the second pulley 24 are both located at the upper parts of the two ends of the rack body 22, the center of the entire tube rack 20 after the culture tube 21 is placed is lower than the axis of the first pulley 23 and the second pulley 24, so that the culture tube 21 together with the tube rack 20 can remain generally stable during movement to reduce shaking. In addition, the structure of the rack body 22 allows the culture tube 21 between the limiting plate and the guide plate to be directly exposed in front of the observation window 13, which is convenient for observation. The rack body 22 has a certain weight, so that it has a greater gravity to drive the first pulley 23 or the second pulley 24 to move.

[0082] In one embodiment, the rack moving device includes two rack moving mechanisms 30 which are parallel to each other. The two rack moving mechanisms 30 are respectively located at two ends of the pipe placement rack 20 .

[0083] The frame moving mechanism 30 includes a downward moving member 31 , an upward moving mechanism 32 , a first limiting mechanism 33 and a driving mechanism 34 .

[0084] The downward moving member 31 is vertically movable and arranged in the box body 10, and is located outside one end of the first strip guide block 15 close to the observation window 13, and has an inclined first guide portion 311 and a blocking portion 312 connected to the lower end of the first guide portion 311. The first guide portion 311 is matched with the second pulley 24, and the first guide portion 311 can receive the second pulley 24 from the lower end of the first strip guide block 15, and the blocking portion 312 can prevent the second pulley 24 from slipping off the first guide portion 311. Specifically, a slider is connected to one end of the blocking portion 312, and a vertical slide groove is provided on the inner wall of the box body 10, and the slider can slide up and down in the slide groove. The length direction of the first guide portion 311 of the downward moving member 31 is arranged parallel to the length direction of the first strip guide block 15.

[0085] The upward movement mechanism 32 can vertically move the tube rack 20 away from the observation window 13 on the second strip guide block 16 to the first strip guide block 15. The driving mechanism 34 is used to drive the downward movement member 31 and the upward movement mechanism 32. The first limiting mechanism 33 can prevent the second pulley 24 on the first strip guide block 15 from disengaging therefrom.

[0086] In this embodiment, after the downward moving member 31 moves upward, its first guide portion 311 docks with the first strip guide block 15, and the pipe rack 20 located on the first strip guide block 15 rolls from the first strip guide block 15 to the first guide portion 311 through the second pulley 24, and then the second pulley 24 is blocked by the blocking portion 312. At this time, the other second pulleys 24 on the first strip guide block 15 follow and move forward, and then are blocked by the first limiting mechanism 33.

[0087] Driven by the driving mechanism 34, the downward moving member 31 moves downward, and then the first guide portion 311 passes through the second strip guide block 16 during the downward movement. During this process, the first guide portion 311 is once at the same level as the second strip guide block 16. Then, during the further downward movement of the downward moving member 31, the first pulley 23 on the tube rack 20 contacts and is supported by the second strip guide block 16, and the first guide portion 311 is separated from the second pulley 24. Since the second strip guide block 16 is tilted, the first pulley 23 drives the tube rack 20 to move along the second strip guide block 16 away from the observation window 13.

[0088] In one embodiment, the upward movement mechanism 32 includes a vertical block 321, a connecting block 322, a stopper 323, a second guide rod 324, a torsion spring 325 and a second limiting mechanism.

[0089] The vertical block 321 is vertically movable in the box body 10, and is located outside the end of the first strip guide block 15 away from the observation window 13. Specifically, the vertical block 321 is installed in the box body 10 in the same manner as the blocking portion 312 is installed in the box body 10.

[0090] The connecting block 322 is connected to the vertical block 321 and extends toward the observation window 13. The connecting block 322 is generally in a horizontal state. The stopper 323 is connected to one end of the connecting block 322 away from the vertical block 321, and the stopper 323 extends along the axial direction of the first pulley 23.

[0091] The second guide rod 324 is matched with the second pulley 24 and can be connected with the first strip guide block 15. One end is rotatably connected with the lower end of the vertical block 321, and the other end can be rotated to be vertical and abut against the upper end of the vertical block 321. The other end can also be rotated to be inclined and abut against the upper end of the stopper 323. Specifically, the second guide rod 324 can rotate within a range of 90 degrees. When its free end is located on the stopper 323, it points to the observation window 13, and at this time, the second guide rod 324 is in an inclined state. The length direction of the second guide rod 324 coincides with the length direction of the first strip guide tube. The torsion spring 325 is arranged on the vertical block 321 to give the end of the second guide rod 324 away from the vertical block 321 a tendency to approach the stopper 323. Specifically, a mounting block is provided on the vertical block 321, and a mounting hole is provided on the mounting block. A rotating shaft is connected to the end of the second guide rod 324, and one end of the rotating shaft is inserted into the mounting hole. The rotating shaft rotates in the mounting hole. A torsion spring 325 is sleeved on the rotating shaft. The torsion spring 325 is arranged in the mounting hole, and one end of the torsion spring 325 is connected to the inner wall of the mounting hole, and the other end is connected to the rotating shaft. The driving mechanism 34 can drive the vertical block 321 to move up and down. The second limiting mechanism can prevent the first pulley 23 on the second guide rod 324 from slipping off

[0092] In this embodiment, the vertical block 321 can move up and down under the drive of the driving mechanism 34. As shown in the figure, the vertical block 321 is in the initial position, at which time the second guide rod 324 is inclined and docked with the first strip guide block 15. As the vertical block 321 moves downward, when passing the lower end of the second strip, the second guide rod 324 abuts against the upper end of the groove of the second pulley 24, and then the second guide rod 324 is supported by the second pulley 24 and begins to rotate counterclockwise on the vertical block 321 until the second guide rod 324 is in a vertical state after rotation. As the vertical block 321 moves further downward, after the second guide rod 324 completely passes the second pulley 24, under the action of the torsion spring 325, the end of the second guide rod 324 away from the vertical block 321 rotates clockwise again and approaches the stopper 323 and abuts against it, and at this time the second pulley 24 is above the second guide rod 324. Then the vertical block 321 moves upward, and the second guide rod 324 approaches from below and contacts and abuts against the lower end of the second pulley 24, thereby forming a support for the second pulley 24. As the vertical block 321 moves further upward, the second pulley 24 moves upward together with the pipe rack 20. At this time, under the obstruction of the second limiting mechanism, the second pulley 24 will not be separated from the second guide rod 324 until the second guide rod 324 is docked with the first strip guide block 15. The second limiting mechanism releases the obstruction, and the second pulley 24 can slide or roll from the second guide rod 324 to the first strip guide block 15.

[0093] In one embodiment, the rack moving mechanism 30 further includes a mounting plate 35 connected to the inner wall of the box body 10 .

[0094] The driving mechanism 34 includes a driving member 341 , a motor 342 and a screw 343 .

[0095] The driving member 341 has two ends connected to the vertical block 321 and the blocking portion 312, respectively, and a driving hole is provided thereon. The driving member 341 is in a strip shape and arranged horizontally. The motor 342 is fixedly mounted on the mounting plate 35. One end of the screw rod 343 is connected to the output end of the motor 342, and the other end of the screw rod 343 passes through the driving hole and is threadedly connected to the driving hole.

[0096] In this embodiment, Figure 2, the screw rod 343 rotates under the drive of the motor 342, thereby driving the driving member 341 to move up and down, thereby driving the vertical block 321 and the blocking part 312 to move up and down. When the driving member 341 moves down, there is no second pulley 24 on the second guide rod 324 on the left side, and there is a second pulley 24 on the first guide part 311 on the right side. As the driving member 341 moves down further, the tube rack 20 on the right side moves down, the first guide part 311 is separated from the second pulley 24, and the second strip guide block 16 contacts and supports the first pulley 23 at the same time. The driving member 341 moves down further, and after the second pulley 24 on the left side passes the second guide rod 324, the driving member 341 moves up, and the second guide rod 324 drives the second pulley 24 there to move up, until the second pulley 24 moves to the height of the first strip guide block 15, and the second slide moves rightward to the first strip guide block 15. The driving member 341 moves downward and upward once, so that the pipe rack 20 on the first strip guide block 15 on the right side is moved to the second strip guide block 16 below, and the storage rack on the second strip guide block 16 below the left side is moved to the first strip guide block 15 above.

[0097] In one embodiment, a mounting groove 351 is formed on one side of the mounting plate 35 facing the observation window 13, and the length direction of the mounting groove 351 is parallel to the axial direction of the first pulley 23. The cross section of the mounting groove 351 may be a convex shape.

[0098] The first limiting mechanism 33 includes a sliding block 331 , a limiting spring 332 , a limiting block 333 and a triggering square rod 334 .

[0099] The sliding block 331 is slidably disposed in the mounting groove 351 and can slide along the length direction of the mounting groove 351 .

[0100] The limiting spring 332 is disposed in the mounting groove 351 , one end of which is connected to the sliding block 331 , and the other end of which is connected to the inner wall of the box body 10 . The limiting spring 332 is a compression spring.

[0101] The limiting block 333 is connected to the sliding block 331 , and the end of the limiting block 333 can move to the upper side of the lower end of the first strip guide block 15 and block the second pulley 24 , and a limiting square hole 335 is vertically penetrated therethrough.

[0102] The lower end of the triggering square rod 334 is connected to the driver, and the upper end of the triggering square rod 334 is provided with a guiding inclined surface 336 .

[0103] The guiding inclined surface 336 can abut against an end portion of the limiting square hole 335 away from the first strip-shaped guide block 15 .

[0104] In this embodiment, Figure 5The upper end of the trigger square rod 334 is located in the limiting square hole 335. At this time, the limiting spring 332 is compressed. As the driving member 341 moves downward, the trigger square rod 334 moves downward, and the guide inclined surface 336 at the upper end of the trigger square rod 334 gradually disengages from the limiting square hole 335, so that the limiting block 333 gradually approaches the second pulley 24 under the drive of the limiting spring 332, until it moves in front of the second pulley 24 to form a block for the second pulley 24, preventing the second pulley 24 from slipping off the first strip guide block 15. At this time, part of the limiting block 333 is against the inclined surface of the first strip guide block 15.

[0105] When the driving member 341 moves upward, the triggering square rod 334 moves upward, and the guiding inclined surface 336 first abuts against the end of the mouth of the limiting square hole 335 away from the first strip guide block 15. The triggering square rod 334 moves upward further, and the upper end of the triggering square rod 334 is gradually inserted into the limiting square hole 335, and the limiting block 333 is driven to gradually move away from the second pulley 24, so that the second pulley 24 can slide normally to the first guide part 311 on the right side, so as to facilitate the observation of the culture tube 21 on the tube rack 20 on the first guide part 311 from the observation window 13, and also prepare for the next downward movement of the tube rack 20.

[0106] In one embodiment, the second limiting mechanism includes a bar-shaped anti-dropping block 326 .

[0107] The upper end of the strip-shaped anti-slipping block 326 is connected to the mounting plate 35 , and the upper end of the strip-shaped anti-slipping block 326 is located between the end of the first strip-shaped guide block 15 away from the observation window 13 and the stopper 323 . Figure 5 In the embodiment of the present invention, one end of the mounting plate 35 is connected to the first strip guide block 15 (i.e., the first strip guide block 15 is connected to the inner wall of the box body 10 through the mounting plate 35), and the upper end of the strip anti-dropping block 326 is also located between the mounting plate 35 and the stopper 323. One end of the second guide rod 324 away from the vertical block 321 is located on the side of the strip anti-dropping block 326. The lower end of the strip anti-dropping block 326 is located above the second pulley 24 of the lower space 12, so as not to hinder the second pulley 24. Until the second guide rod 324 lifts the second pulley 24, the strip anti-dropping block 326 can block the lifted second pulley 24 to prevent the second pulley 24 from slipping off the second guide rod 324.

[0108] In the description of the present invention, a large number of specific details are described. However, it is understood that embodiments of the present invention can be practiced without these specific details. In some instances, well-known methods, structures and techniques are not shown in detail so as not to obscure the understanding of this description.

[0109] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and specification of the present invention.

Claims

1. A stem cell culture device, characterized in that: include: An openable and closable box (10) has an upper space (11) and a lower space (12) therein, and an observation window (13) is provided on one side thereof; Two rows of tube racks (20) arranged in an upper and lower manner are respectively located in the upper space (11) and the lower space (12); a plurality of culture tubes (21) can be placed horizontally on each of the tube racks (20); the tube racks (20) in the upper space (11) can be moved successively closer to the observation window (13); and the tube racks (20) in the lower space (12) can be moved successively away from the observation window (13); and a rack moving device, which can vertically move the pipe rack (20) located in the upper space (11) close to the observation window (13) to the lower space (12), and can vertically move the pipe rack (20) located in the lower space (12) far from the observation window (13) to the upper space (11); The box (10) comprises: two side plates (14) parallel to each other; Two mutually parallel first strip guide blocks (15), one end of which is close to the observation window (13) and is inclined downward, and each is located on the inner side of the side plate (14) on the same side; and Two second strip guide blocks (16) parallel to each other, one end of which is away from the observation window (13) and is tilted downward, located below the first strip guide block (15), and each located on the inner side of the side plate (14) on the same side; Both ends of the tube placement rack (20) can slide on the first strip guide block (15) or the second strip guide block (16); The tube placement rack (20) comprises: Frame body (22); Two coaxial first pulleys (23) are rotatably connected to the upper parts of both ends of the frame body (22) and can fit with the second strip guide block (16) on the same side; and Two coaxial second pulleys (24) are rotatably connected to the upper parts of both ends of the frame body (22), are coaxial with the first pulley (23), and can fit with the first strip guide block (15) on the same side; The rack moving device comprises two rack moving mechanisms (30) which are parallel to each other. The two rack moving mechanisms (30) are respectively located at two ends of the pipe placement rack (20). The rack moving mechanisms (30) comprise: A downward moving member (31) is vertically movable and arranged in the box body (10), located outside one end of the first strip guide block (15) close to the observation window (13), and comprises an inclined first guide portion (311) and a blocking portion (312) connected to the lower end of the first guide portion (311); An upward movement mechanism (32) capable of vertically moving the tube placement rack (20) on the second strip guide block (16) away from the observation window (13) to the first strip guide block (15); a first limiting mechanism (33) capable of preventing the second pulley (24) on the first strip guide block (15) from being disengaged therefrom; and A driving mechanism (34) for driving the downward moving member (31) and the upward moving mechanism (32); The first guide portion (311) is matched with the second pulley (24); the first guide portion (311) can receive the second pulley (24) from the lower end of the first strip guide block (15); and the blocking portion (312) can prevent the second pulley (24) from slipping off the first guide portion (311); The upward movement mechanism (32) comprises: A vertical block (321) is vertically movably disposed in the box body (10) and is located outside an end of the first strip guide block (15) away from the observation window (13); A connecting block (322) connected to the vertical block (321) and extending toward the observation window (13); A stopper (323) connected to an end of the connecting block (322) away from the vertical block (321); A second guide rod (324) is matched with the second pulley (24) and can be connected to the first strip guide block (15). One end of the second guide rod (324) is rotatably connected to the lower end of the vertical block (321). The other end of the second guide rod (324) can be rotated to be vertical and abut against the upper end of the vertical block (321). The other end of the second guide rod (324) can also be rotated to be inclined and abut against the upper end of the stop block (323). a torsion spring (325) disposed on the vertical block (321) to give the end of the second guide rod (324) away from the vertical block (321) a tendency to approach the stop block (323); and a second limiting mechanism, capable of preventing the first pulley (23) on the second guide rod (324) from slipping off; The driving mechanism (34) can drive the vertical block (321) to move up and down; The rack moving mechanism (30) further comprises a mounting plate (35) connected to the inner wall of the box body (10), and the driving mechanism (34) comprises: A driving member (341), two ends of which are respectively connected to the vertical block (321) and the blocking portion (312), and a driving hole is provided on the driving member; A motor (342) mounted on the mounting plate (35); A screw rod (343), one end of which is connected to the output end of the motor (342), and the other end of which passes through the driving hole and is threadedly connected to the driving hole; A mounting groove (351) is formed on a side of the mounting plate (35) facing the observation window (13); The first limiting mechanism (33) comprises: A sliding block (331) is slidably disposed in the mounting groove (351) and can slide along the length direction of the mounting groove (351); A limit spring (332) is arranged in the installation groove (351), one end of which is connected to the sliding block (331) and the other end of which is connected to the inner wall of the box body (10); A limiting block (333) connected to the sliding block (331), an end of which can be moved to the upper side of the lower end of the first strip guide block (15) and form a block for the second pulley (24), and a limiting square hole (335) vertically penetrating therethrough is provided on the limiting block; and A trigger square rod (334), the lower end of which is connected to the drive, and the upper end of which is provided with a guide inclined surface (336); The guiding inclined surface (336) can abut against an end portion of the limiting square hole (335) away from the first strip-shaped guide block (15); The second limiting mechanism comprises: a strip-shaped anti-slip block (326), the upper end of which is connected to the mounting plate (35), the upper end of which is located between an end of the first strip-shaped guide block (15) away from the observation window (13) and the stopper (323), and the lower end of which is located above the second pulley (24) in the lower space (12); The strip-shaped anti-slip block (326) can block the lifted second pulley (24).

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