Penicillium oxalicum phfg1220 and use thereof

Abstract

The present application relates to the field of microbial technology, and particularly relates to a strain of penicillium oxalicum PHFG1220 and application thereof, the penicillium oxalicum PHFG1220 of the present application is preserved in the China General Microbiological Culture Collection Center on December 4, 2023, and the preservation number is CGMCC No.41001.The penicillium oxalicum PHFG1220 of the present application is isolated from the soil of rotten citrus waste fruits in Pinghe County, Zhangzhou City, Fujian Province, has strong growth adaptability, high acid and alkali tolerance, is not sensitive to the change of pH and temperature, is easy to grow, is suitable for large-scale production, and the strain forms round mycelium globules, is easy to adsorb on the surface of waste fruits, prevents the pollution of waste fruits by miscellaneous bacteria, and has good degradation effect.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microbial technology, in particular to a strain of Penicillium oxalicum PHFG1220 and its application, more particularly to a strain of Penicillium oxalicum PHFG1220 capable of degrading honey pomelo waste fruits and its application. BACKGROUND

[0002] In recent years, the fruit industry in China has developed rapidly, resulting in a large amount of fruit waste. Improper disposal of fruit waste has caused serious threats to the environment and human health, such as farmland, orchard, water body, etc. Honey pomelo is a famous fruit in Pinghe County, Fujian Province, which contains rich vitamin C and a large amount of nutrients. It is renowned at home and abroad for its large size, juicy, sweet and sour taste, and other excellent qualities. It is a unique and excellent citrus variety in China. However, a large amount of honey pomelo waste, such as diseased and insect-infested fruits, culls, fallen fruits, pomelo pomace and non-edible parts, is generated during the production, processing and consumption of honey pomelo, accounting for 20% of the total yield. Due to the high cost of processing waste fruits, the waste fruits are often randomly piled up in the wild and abandoned orchards, leading to rotting and producing a foul odor, which seriously affects the urban and rural environment. In addition, with the acceleration of social life pace, the proportion of fruit processing products in the overall fruit consumption structure is also increasing, resulting in more pomace as a byproduct of processing.

[0003] Honey pomelo waste and pomace contain rich nutrients, and honey pomelo pomace contains crude protein, crude fat, crude fiber and is rich in various amino acids, which can provide good substances for microorganisms. The main component of honey pomelo pomace is cellulose, which has a tight and orderly arrangement of glucose subunits, forming a water-impermeable structure similar to a crystal. Honey pomelo cellulose cannot be dissolved in water, which seriously limits the utilization of honey pomelo pomace. Using microorganisms to treat honey pomelo waste and pomace to reduce cellulose content has the advantages of low investment, simple operation and no environmental pollution, and has become a research hotspot in recent years.

[0004] Research has found that microorganisms that can produce cellulase in the environment mainly include bacteria, fungi and actinomycetes. Compared with bacteria and actinomycetes, fungi produce extracellular enzymes, which are easy to separate and extract, have high enzyme activity, and have a comprehensive composition of enzyme production system, can secrete a large amount of hemicellulase, pectinase, amylase and other extracellular enzymes at the same time, and have been widely used in industrial enzyme production, biological medicine and other industries. Penicillium oxalicum can secrete various extracellular enzymes, especially high-activity cellulase and xylosidase, which can efficiently degrade cellulose in waste fruits. In addition, some soil Penicillium oxalicum can secrete small molecule secondary metabolites, promote the absorption of zinc, copper and other nutrients by plant roots, and enhance the plant's disease resistance. However, there is no related research report on the degradation of honey pomelo waste by Penicillium oxalicum and the growth-promoting effect of the degradation of waste fruit fermentation broth on plants. SUMMARY

[0005] The present application aims at the deficiencies of the prior art, and provides a strain of Penicillium oxalicum PHFG1220 and an application thereof, the Penicillium oxalicum PHFG1220 being isolated from rotten citrus waste fruit soil in Pinghe County, Zhangzhou City, Fujian Province, having strong growth adaptability, high acid-base tolerance, being not sensitive to changes in pH and temperature, being easy to grow, being suitable for large-scale production, and forming round mycelium balls, being easy to adsorb on the surface of waste fruit, preventing contamination of waste fruit by miscellaneous bacteria, and having good degradation effect.

[0006] To achieve the above object, the present application provides a strain of Penicillium oxalicum PHFG1220 in a first aspect, the Penicillium oxalicum PHFG1220 being classified and named as Penicillium oxalicum, and being preserved in the China General Microbiological Culture Collection Center on December 4, 2023, with a preservation number of CGMCC No.41001 and a preservation address of No.3, Yitianxili, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences.

[0007] The Penicillium oxalicum PHFG1220 strain grows slowly on a PDA plate, the colony has a flat texture and a woolly shape, the mycelium is white in the early stage and turns into gray-green in the later stage, water droplets are exuded, a large number of conidial structures are produced and easily fall off, and no soluble pigment is produced. The conidiophore occurs in the substrate, the wall is smooth, the broom-shaped branches are double-wheel-shaped and relatively close; the conidium is oval, the wall is smooth, the size of the conidium is 3.6-5.3 μm x 2.7-3.6 μm; and the conidial chain is cylindrical.

[0008] Further, the Penicillium oxalicum PHFG1220 has a nucleotide sequence as shown in SEQ ID NO. 1.

[0009] In a second aspect, the present application provides a fermentation liquor of the Penicillium oxalicum PHFG1220, the fermentation liquor of the Penicillium oxalicum PHFG1220 being obtained after the above-mentioned Penicillium oxalicum PHFG1220 is cultured, and the specific culture method is as follows: the Penicillium oxalicum PHFG1220 strain is inoculated on a PDA agar culture medium to activate, and the mycelium of the Penicillium oxalicum PHFG1220 is obtained; then the mycelium of the Penicillium oxalicum PHFG1220 is picked and inoculated into a YPD liquid culture medium, and is cultured at a shaking speed of 150-180 rpm at 28℃ for 36-48 h.

[0010] Further, the PDA agar culture medium comprises 200 g of peeled potatoes, 15-25 g of glucose, 13 g of agar powder and 1000 mL of distilled water, and the pH is natural; and the YPD liquid culture medium comprises 15-25 g of glucose, 20 g of peptone and 10 g of yeast extract, and 1000 mL of distilled water, and the pH is natural.

[0011] In a third aspect of the present application, the application provides a use of the above-mentioned Penicillium oxalicum PHFG1220 or the fermentation liquor of the Penicillium oxalicum PHFG1220 in degrading pomelo waste fruits. The Penicillium oxalicum PHFG1220 can grow on a CMC-Congo red culture medium and produce a transparent circle, has good cellulase activity and cellulose degradation capacity, and can disintegrate filter paper and has strong filter paper enzyme activity.

[0012] In a fourth aspect of the present application, the application provides a Penicillium oxalicum fermentation liquor obtained by degrading pomelo waste fruits by the Penicillium oxalicum PHFG1220. The preparation method of the Penicillium oxalicum fermentation liquor is as follows: the Penicillium oxalicum PHFG1220 is cultured into a fermentation liquor first generation inoculum, and then is transferred into pomelo waste fruits for expansion culture, or the fermentation liquor of the Penicillium oxalicum PHFG1220 is directly transferred into pomelo waste fruits for expansion culture, to obtain a Penicillium oxalicum PHFG1220 second generation inoculum, and then the Penicillium oxalicum PHFG1220 second generation inoculum is covered on the surface of the broken pomelo waste fruits in a culture barrel for anaerobic fermentation, to obtain the Penicillium oxalicum fermentation liquor.

[0013] In a fifth aspect of the present application, the application provides a use of the Penicillium oxalicum PHFG1220 fermentation liquor in degrading pomelo waste fruits in preparing a plant growth promoting preparation. Preferably, the Penicillium oxalicum fermentation liquor is used after being diluted by 1 to 300 times.

[0014] Further, the application provides a use of the Penicillium oxalicum PHFG1220 fermentation liquor in degrading pomelo waste fruits in preparing a tea growth promoting preparation.

[0015] In a sixth aspect of the present application, the application provides a preparation for degrading pomelo waste fruits, which comprises the above-mentioned Penicillium oxalicum PHFG1220 or the above-mentioned Penicillium oxalicum PHFG1220 fermentation liquor.

[0016] In a seventh aspect of the present application, the application provides an organic fertilizer comprising the above-mentioned Penicillium oxalicum fermentation liquor.

[0017] The present application has the following advantages:

[0018] In the present application, a strain of Penicillium oxalicum PHFG1220 is obtained by microbial separation and transparent circle hydrolysis experiment on rotten citrus waste fruit soil. The strain is harmless to human and animals, has good degradation effect and good application prospect. The fermentation liquor of the Penicillium oxalicum PHFG1220 has good degradation effect on pomelo waste fruits, can quickly degrade cellulose in pomelo waste fruits, and can completely degrade cellulose in pomelo waste fruits after 26 days under natural environmental conditions. Meanwhile, the Penicillium oxalicum PHFG1220 fermentation liquor has the effect of promoting the growth of tea and other plants.

[0019] The oxalic acid penicillium PHFG1220 strain provided by the application can efficiently degrade honey pomelo waste fruits, the honey pomelo waste fruit fermentation liquor can promote the growth of plants, the cost is low, the environment polluted by the waste fruits is improved, an effective technology for recycling fruit waste is provided, the waste fruits are converted into organic fertilizer which can promote the growth of plants, the soil fertility and ecological environment can be improved. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 Hydrolysis diagram of transparent circle of the oxalic acid penicillium PHFG1220 of the application;

[0021] Figure 2 Colony morphology result diagram of the oxalic acid penicillium PHFG1220 of the application growing on PDA;

[0022] Figure 3 Microscopic irradiation result diagram of the oxalic acid penicillium PHFG1220 of the application;

[0023] Figure 4 Phylogenetic tree of the oxalic acid penicillium PHFG1220 of the application;

[0024] Figure 5 Filter paper degradation result diagram of the oxalic acid penicillium PHFG1220 of the application, wherein the left side is a control and the right side is the PHFG1220 fermentation liquor;

[0025] Figure 6 Honey pomelo waste fruit degradation process diagram of the application;

[0026] Figure 7 Honey pomelo waste fruit degradation result diagram of the oxalic acid penicillium PHFG1220 of the application;

[0027] Figure 8 Honey pomelo waste fruit degradation result diagram of the oxalic acid penicillium PHFG1220 of the application in secondary fermentation;

[0028] Figure 9 Promoting effect diagram of the honey pomelo waste fruit degradation fermentation liquor on tea.

[0029] The application will be further described below in combination with the drawings and examples. DETAILED DESCRIPTION

[0030] In order to better illustrate the technical solutions of the application, the solutions of the application will be explained below in combination with examples. Those skilled in the art will understand that the following examples are only used to illustrate the application and should not be regarded as limiting the scope of the application. If the specific technology or condition is not specified in the examples, the technology described in the literature or reference books or according to the product instruction is used. If the manufacturer of the reagent or instrument is not specified, it is a conventional product, and if the reagent is not specifically specified, it is prepared by a conventional method.

[0031] The above technical features of the present application and the technical features described in detail below (such as the embodiments) can be combined with each other to form new or preferred technical solutions.

[0032] Example 1: Screening of cellulose-degrading bacterial strains

[0033] Enrichment medium: 10 g of proteose peptone, 1 g of K2HPO4, 5 g of Na2CO3, 0.1 g of MgSO4·7H2O, 0.015 g of FeSO4·7H2O, 0.05 g of MnSO4 (0.056 g of MnSO4·H2O), 10 g of yeast extract, 1000 mL of distilled water, and pH 6.0.

[0034] Screening medium: 10 g of sodium carboxymethyl cellulose (CMC-Na), 1 g of KNO3, 0.5 g of K2HPO4, 1.5 g of NaCl, 0.5 g of MgSO4·7H2O, 13 g of agar powder, and 1000 mL of distilled water.

[0035] Seed medium (PDA agar medium): 200 g of peeled potatoes, 20 g of glucose, 13 g of agar powder, 1000 mL of distilled water, and natural pH.

[0036] Primary screening: 10 g of the sample was added to 90 mL of sterilized enrichment medium, and incubated at 200 r / min and 28°C for 10 d. Then, 10 mL of the liquid was centrifuged at 4000 r / min for 10 min, 1 mL of the supernatant was gradiently diluted with sterile water, and 0.1 mL of each diluted sample was inoculated on the screening medium, with 3 repeats for each gradient. After incubation at 28°C for 3-5 d, single colonies were picked and further isolated and purified on the screening medium.

[0037] Secondary screening: the purified colonies were inoculated on the screening medium, with 3 repeats, and incubated at 28°C for 4 d. Then, the colonies were dyed with 1 g / L of Congo red solution for 1 h, the dye solution was discarded, and 1 mol / L of NaCl solution was added for washing for 1 h. The presence of obvious transparent hydrolysis rings around the colonies was observed, and the diameters of the transparent rings (D, cm) and the colonies (d, cm) were measured and statistically analyzed. The degradation capacity was represented by Dp: Dp=(D / d) 2 .

[0038] The results showed that the strain PHFG1220 produced obvious transparent hydrolysis rings around the colonies, as shown in Figure 1 , and the Dp value was 2, indicating that the strain PHFG1220 had good cellulase activity and cellulose degradation capacity.

[0039] The PHFG1220 strain obtained by screening was inoculated into PDA agar medium and incubated at 28°C. The PHFG1220 strain was preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 4, 2023, with a preservation number of CGMCC No. 41001 and a preservation address of No. 3, Yikhina West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, with a postcode of 100101.

[0040] Example 2: Identification of a cellulose-degrading strain

[0041] Czapek agar medium: sucrose 30 g, NaNO3 3 g, K2HPO4 1 g, KCl 0.5 g, MgSO4·7H2O 0.5 g, FeSO4·7H2O 0.01 g, agar powder 15 g, distilled water 1000 mL.

[0042] The PHFG1220 strain was inoculated into PDA agar medium and incubated at 28°C, and the colony morphological characteristics were observed. The PHFG1220 strain was inoculated into Czapek agar medium, and after the strain produced spores, it was observed under a microscope. The colony morphological characteristics and microscopic irradiation results of the strain PHFG1220 are shown in Figure 2 and Figure 3 The colony grew slowly on the PDA plate, the colony texture was flat and villous, the mycelium was white in the early stage and turned to gray-green in the later stage, with water droplets exuding, a large number of conidial structures were produced and easily detached, and no soluble pigment was produced. Conidiophores occurred in the substrate, the wall was smooth, the broom-shaped branches were double-wheel, and relatively close; the conidia were oval, the wall was smooth, the size of the conidia was 3.6-5.3 μm x 2.7-3.6 μm; the conidial chain was cylindrical.

[0043] The PHFG1220 strain was inoculated into PDA agar medium and incubated at 28°C for 5-6 days, and then sent to Shengong Bioengineering (Shanghai) Co., Ltd. for strain identification.

[0044] The genomic DNA of the strain PHFG1220 was extracted by using the SK8259 kit, and was used as the template for PCR amplification. The ITS sequence was amplified by using the primers ITS1 (SEQ ID NO. 2): 5'-TCCGTAGGTGAACCTGCGG-3' / ITS4 (SEQ ID NO. 3): 5'-TCCTCCGCTTATTGATATGC-3'. The target DNA fragment was purified and recovered by using a gel purification kit (AXYGEN), and was sequenced after ligation, transformation and identification, to obtain a full-length sequence of 574 bp, as shown in SEQ ID NO. 1. The sequencing results were subjected to BLAST sequence alignment on the NCBI website, and a rDNA-ITS phylogenetic tree was constructed by using the software MEGA7.0 and the neighbor-joining method, as shown in FIG. 1. The strain PHFG1220 belongs to the same branch as Penicillium oxalicum (genetic accession number MT597864.1), with a similarity of 99.65%. Based on the morphological characteristics and culture characteristics, the strain PHFG1220 was identified as Penicillium oxalicum. Figure 4

[0045] Example 3: Filter paper degradation assay of the cellulose-degrading strain

[0046] Culture method of Penicillium oxalicum PHFG1220: The Penicillium oxalicum PHFG1220 strain preserved in the ultra-low temperature refrigerator was inoculated on PDA agar medium, and was cultured at 28°C for 5-7 days for activation, to obtain the mycelium of Penicillium oxalicum PHFG1220. The mycelium of Penicillium oxalicum PHFG1220 was inoculated into YPD medium, and was cultured at 28°C with shaking at a speed of 180 rpm for 36 h, to obtain the fermentation broth of Penicillium oxalicum PHFG1220.

[0047] The PDA agar medium comprises 200 g of peeled potatoes, 20 g of glucose, 13 g of agar powder and 1000 mL of distilled water, and has a natural pH. The YPD liquid medium comprises 20 g of glucose, 20 g of peptone and 10 g of yeast extract, and has a natural pH.

[0048] ​The filter paper strip culture medium is: NaNO3 2.5 g, K2HPO4 1 g, CaCl2·6H2O 0.1 g (CaCl2·2H2O 0.067 g), MgSO4 0.3 g, NaCl 0.1 g, FeCl3 0.01 g (FeCl3·6H2O 0.0166 g), add distilled water to 1000 mL, pH naturally, take 50 mL of the culture medium in a conical flask, add filter paper strip 0.5 g, 121 ℃ high pressure sterilization for 20 min. After sterilization, take 1 mL of the Penicillium oxalicum PHFG1220 fermentation liquor to inoculate in the conical flask, and place in a constant temperature incubator at 28 ℃.

[0049] The results are shown in Figure 5 As shown (left side is the control, right side is the PHFG1220 fermentation liquor), the PHFG1220 strain can also disintegrate filter paper, indicating that the cellulase activity is very high.

[0050] Example 4: Study on the degradation of pomelo waste by the cellulose-degrading strain

[0051] The pomelo waste is crushed, and 200 g of the crushed pomelo waste is respectively taken and placed in 42 500 mL glass bottles, which are sterilized at 121 ℃ for 20 min. The Penicillium oxalicum PHFG1220 fermentation liquor obtained in Example 3 is inoculated in the glass bottles containing pomelo waste, and is covered on the surface of the pomelo waste, which is placed in a constant temperature incubator at 28 ℃ until the pomelo waste is degraded into soup, and meanwhile, the sample is taken every 2 days for solid-liquid separation (three times of repetition), and the pomelo waste degradation rate is analyzed. The specific steps are as follows: the pomelo waste residue is weighed, and the pomelo waste degradation rate is calculated.

[0052] The degradation rate = (wet weight of pomelo waste before fermentation - wet weight of pomelo residue after fermentation) / wet weight of pomelo waste before fermentation × 100%

[0053] The results show that after the Penicillium oxalicum PHFG1220 is fermented to degrade the pomelo waste for 2 d, 4 d, 6 d, 8 d, 10 d, 12 d, 14 d, 16 d, 18 d, 20 d, 22 d, 24 d and 26 d, the wet weight of pomelo waste residue is 190.6 g, 173.4 g, 159.7 g, 147.3 g, 135.5 g, 124.6 g, 114.4 g, 105.5 g, 93.4 g, 70.5 g, 45.3 g, 22.7 g and 4.4 g, respectively, the pomelo waste degradation rate is 4.7%, 13.3%, 20.2%, 26.4%, 32.3%, 37.7%, 42.8%, 47.2%, 53.3%, 64.7%, 77.3%, 88.7% and 97.8%, respectively, and the pomelo waste degradation process is as shown in Figure 6The results show that Penicillium oxalicum PHFG1220 has strong degradation ability to pomelo waste fruits. The strain proliferates rapidly in the first 5 days, then the strain proliferates while the pomelo waste fruits gradually soften and degrade, and the pomelo waste fruits are completely degraded into soup liquid on the 26th day. With the extension of fermentation time, the color of the fermentation liquid gradually deepens, and the degradation results of pomelo waste fruits after 60 days of fermentation are shown in Table 2. Figure 7

[0054] The results show that Penicillium oxalicum PHFG1220 has strong degradation ability to pomelo waste fruits. The strain proliferates rapidly in the first 5 days, then the strain proliferates while the pomelo waste fruits gradually soften and degrade, and the pomelo waste fruits are completely degraded into soup liquid on the 26th day. With the extension of fermentation time, the color of the fermentation liquid gradually deepens, and the degradation results of pomelo waste fruits after 60 days of fermentation are shown in Table 2. Figure 8

[0055] The results show that Penicillium oxalicum PHFG1220 has strong degradation ability to pomelo waste fruits. The strain proliferates rapidly in the first 5 days, then the strain proliferates while the pomelo waste fruits gradually soften and degrade, and the pomelo waste fruits are completely degraded into soup liquid on the 26th day. With the extension of fermentation time, the color of the fermentation liquid gradually deepens, and the degradation results of pomelo waste fruits after 60 days of fermentation are shown in Table 2.

[0056] Example 5: Promoting effect of degradation pomelo waste fruit fermentation liquid on tea

[0057] A plot test was set up with three treatments, namely, degradation pomelo waste fruit fermentation liquid 150 times liquid experimental group, degradation pomelo waste fruit fermentation liquid 300 times liquid experimental group, and conventional chemical fertilizer control group CK. Healthy tea trees of five-year-old with good growth and uniform size were selected, and the test groups were sprayed with degradation pomelo waste fruit fermentation liquid 150 times liquid and 300 times liquid once when the tea buds were about 1 cm long. The control group was sprayed with seaweed fertilizer 100 times liquid once, and the spraying was carried out every 10 days. Regular management was carried out until 10 days before harvesting.

[0058] The length of the teeth, bud density, 100 bud weight, leaf length, leaf width, leaf thickness, and yield per mu of tea were measured, as well as the tea quality indicators such as water extract, caffeine, tea polyphenol, catechin, total free amino acid, and phenol-ammonia ratio.

[0059] ​​Bud length: 30 tea buds were collected according to the standard of one bud and three leaves, the length from the top of the bud to the base of the third leaf was measured, 3 replicates were performed for each treatment, and the average value was taken; bud density: a 33.3 cm x 33.3 cm quadrat was randomly selected, all the buds were picked and counted to calculate the total number, 3 replicates were performed for each treatment, and the average value was taken; 100-bud weight: tea buds were randomly picked according to the standard of one bud and three leaves, the weight of 100 tea buds was measured, 3 replicates were performed for each treatment, and the average value was taken; leaf length, leaf width, and leaf thickness: 30 samples were collected according to the standard of one bud and three leaves, the longitudinal distance from the base of the third leaf to the tip was measured as the leaf length, the transverse distance at the middle of the third leaf was measured as the leaf width, and the thickness at the middle of the third leaf was measured as the leaf thickness, 3 replicates were performed for each treatment, and the average value was taken; yield per mu: all the tea leaves in the plot were picked and weighed, and the yield per mu was estimated according to the area.

[0060] Determination of water extract: boiling water extraction and drying method (GB / T 8305-2013); determination of caffeine: high performance liquid chromatography (GB / T 8312-2013); determination of tea polyphenols: Folin phenol reagent colorimetric method (GB / T 8313-2018); determination of catechins: HPLC method (GB / T 8313-2018); total amount of free amino acids: indantrione colorimetric method (GB / T 8314-2013); phenol-ammonia ratio calculation formula: phenol-ammonia ratio = tea polyphenols / total amount of free amino acids.

[0061] The results showed that the fermented liquid of degraded honey pomelo waste fruits had different degrees of promoting effect on the bud length, bud density, leaf length, leaf width, leaf thickness, and tea yield of tea leaves, and the results are shown in Table 1. Figure 9 The tea yield of the tea leaves treated with 150 times of the fermented liquid was 894.63 kg / 667m 2 , which was 205.41 kg / 667m 2 higher than that of the control group, with an increase rate of 29.83%; the tea yield of the tea leaves treated with 300 times of the fermented liquid was 835.66 kg / 667m 2 , with an increase rate of 21.26% (as shown in Table 1). It was shown that the fermented liquid of degraded honey pomelo waste fruits could effectively promote the growth of tea leaves and increase the yield of tea leaves.

[0062] The degradation of honey pomelo waste fruit fermentation liquor has certain influence on the content of tea water extract, caffeine, catechin, tea polyphenol and free amino acid (as shown in Table 2). The tea water extract of 150 times diluted fermentation liquor and 300 times diluted fermentation liquor is increased by 2.81% and 1.00%, respectively; the caffeine is increased by 12.29% and 8.47%, respectively; the catechin is increased by 13.00% and 5.38%, respectively; the tea polyphenol is increased by 3.59% and 1.50%, respectively; the free amino acid is increased by 11.00% and 9.00%, respectively; and the tea phenol-ammonia ratio is reduced by 6.72% and 7.46%, respectively. The results show that the application of the degradation of honey pomelo waste fruit fermentation liquor can effectively promote the formation of tea fresh and beneficial substances, make the tea soup taste fresh and refreshing, and improve the quality of tea.

[0063] Table 1: Determination results of tea morphological indexes

[0064]

[0065] Note: The data in the table is the average ± standard deviation. The same column data with different lowercase letters indicates that the difference is significant at the P<0.05 level by Duncan's new multiple range test.

[0066] Table 2: Determination results of tea physicochemical indexes

[0067]

[0068] Note: The data in the table is the average ± standard deviation. The same column data with different lowercase letters indicates that the difference is significant at the P<0.05 level by Duncan's new multiple range test.

[0069] Finally, it needs to be emphasized that the above is only the preferred embodiment of the present application and is not used to limit the present application. For those skilled in the art, the present application can have various changes and modifications, and any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A strain of Penicillium oxalicum ( Penicillium oxalicum ) PHFG1220, characterized in that The deposit number of the Penicillium oxalicum PHFG1220 is CGMCC No.41001.

2. A fermentation broth of Penicillium oxalicum PHFG1220, characterized in that: The fermentation broth of Penicillium oxalicum PHFG1220 is obtained by culturing the Penicillium oxalicum PHFG1220 according to claim 1. The specific culturing method is: first, the Penicillium oxalicum PHFG1220 strain is inoculated onto a PDA agar medium for activation to obtain Penicillium oxalicum PHFG1220 mycelium; then, the Penicillium oxalicum PHFG1220 mycelium is picked and inoculated into a YPD liquid culture medium, and the fermentation broth is shaken and cultured at 28° C. for 36 to 48 hours on a shaker with a rotation speed of 150 to 180 rpm.

3. The fermentation broth of Penicillium oxalicum PHFG1220 according to claim 2, characterized in that The components of the PDA agar medium are: 200 g of peeled potatoes, 15-25 g of glucose, 13 g of agar powder, 1000 mL of distilled water, and a natural pH value; the components of the YPD liquid medium are: 15-25 g of glucose, 20 g of peptone, 10 g of yeast extract, 1000 mL of distilled water, and a natural pH value.

4. Use of the Penicillium oxalicum PHFG1220 according to claim 1 or the fermentation liquid of Penicillium oxalicum PHFG1220 according to claim 2 or 3 in degrading waste pomelo fruit.

5. A fermentation liquid for degradation of pomelo waste fruit by Penicillium oxalicum PHFG1220, characterized in that: The specific preparation method of the fermentation liquid of Penicillium oxalate PHFG1220 for degrading honey pomelo waste fruit is as follows: Penicillium oxalate PHFG1220 is cultured to form Penicillium oxalate PHFG1220 fermentation liquid, which is then transferred to honey pomelo waste fruit for expanded culture to obtain a secondary inoculant of Penicillium oxalate PHFG1220, and the secondary inoculant of Penicillium oxalate PHFG1220 is then covered on the surface of the crushed honey pomelo waste fruit in a culture barrel for anaerobic fermentation.

6. Use of the fermentation liquid of waste pomelo fruit degraded by Penicillium oxalicum PHFG1220 as claimed in claim 5 in the preparation of a preparation for promoting tea growth.

7. A preparation for degrading waste pomelo fruit, characterized in that: The method comprises the Penicillium oxalicum PHFG1220 according to claim 1 or the fermentation broth of Penicillium oxalicum PHFG1220 according to claim 2 or 3.

8. An organic fertilizer comprising the fermentation liquid of pomelo waste fruit degraded by Penicillium oxalicum PHFG1220 according to claim 5.

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