Composition with efficacy of preventing hair loss and promoting hair growth and application thereof

Abstract

The present invention discloses a composition having the efficacy of preventing hair loss and promoting hair growth, and its application. By combining ginsenoside CK and biochanin A, the effects of preventing hair loss and promoting hair growth and their synergistic effects are achieved. The specific technical solution of the present invention is as follows: a composition having the efficacy of preventing hair loss and promoting hair growth, comprising ginsenoside CK and biochanin A; the weight ratio of ginsenoside CK to biochanin A is 1:0.5-3. The composition can be used in the preparation of anti-hair loss products. The present invention combines ginsenoside CK and biochanin A. The combination of the two promotes hair growth primarily by enhancing the antioxidant capacity of mouse skin tissue. Within a certain ratio range, the antioxidant capacity of mouse skin tissue can be synergistically improved. Within a certain ratio range, ginsenoside CK and biochanin A can synergistically promote hair growth.

Description

Technical Field

[0001] The present invention belongs to the field of biomedicine and relates to a composition having the effects of preventing hair loss and promoting hair growth and an application thereof. Background Art

[0002] Hair loss refers to the loss or cessation of hair growth in certain areas of the scalp. Due to factors such as accelerated work pace, increased mental stress, hormonal imbalances, and aging, the incidence of hair loss is increasing year by year, and the ageing population is experiencing hair loss. According to statistics, 63.1% of people under the age of 35 experience hair loss. Hair loss is highly likely to cause self-perception impairment and psychological conflict in young people. Therefore, healthy hair is crucial for maintaining a healthy mind. Currently, surgical and drug treatments are the primary medical options for treating and preventing hair loss. Surgical treatments have drawbacks such as being time-consuming, expensive, and requiring limited access to follicular units. Western medications such as finasteride and minoxidil are limited by their significant adverse reactions, high recurrence rates, and long treatment cycles. Furthermore, Chinese herbal medicines have also been used in recent years to prevent and treat hair loss and promote hair growth, but they still suffer from complex components and a long time to take effect. Consequently, the development of safe and effective compositions for the treatment and prevention of hair loss is gaining increasing attention.

[0003] Currently, methods for preventing or treating hair loss remain limited. Ginsenoside CK, a rare ginsenoside, has demonstrated some effectiveness in inhibiting androgenic alopecia. This substance, obtained by deglycosylation of naturally abundant ginsenosides such as Rb1, is a tetracyclic dammarane-type triterpenoid saponin. However, current research has largely focused on the use of ginsenoside CK alone, and its synergistic effects with other components have not been fully investigated or reported.

[0004] Meanwhile, biochanin A, a naturally active product found in high concentrations in Trifolium pratense, possesses a variety of biological properties, including anti-inflammatory, antioxidant, and endocrine regulation. More importantly, biochanin A can inhibit 5α-reductase activity, reducing dihydrotestosterone (DHT) production, thereby promoting hair growth in mice. Despite this, existing applications primarily focus on the use of biochanin A alone, and its synergistic effects with other compounds require further investigation. Summary of the Invention

[0005] There are currently no reports on the efficacy of the combined use of ginsenoside CK and biochanin A in preventing hair loss and promoting hair growth, as well as their synergistic effects. The present invention aims to provide a composition that prevents hair loss and promotes hair growth, specifically focusing on the efficacy of the combined use of ginsenoside CK and biochanin A in preventing hair loss and promoting hair growth, as well as their synergistic effects. Given the potential of these two compounds in preventing hair loss, studying their combined use may be of great significance and could potentially provide a more effective solution to the problem of hair loss.

[0006] The technical solution adopted in the present invention is:

[0007] A composition with the effects of preventing hair loss and promoting hair growth comprises ginsenoside CK and biochanin A.

[0008] The weight ratio of ginsenoside CK to biochanin A is 1:0.5-3.

[0009] The chemical formula of ginsenoside CK is: .

[0010] The chemical formula of the biochanin A is: .

[0011] The composition further comprises external cleaning product auxiliary materials, which are one or more combinations of foaming agents, surfactants, emulsifiers, thickeners, preservatives, and fragrances.

[0012] The application of the above composition with the efficacy of preventing hair loss and promoting hair growth in the preparation of anti-hair loss products.

[0013] The anti-hair loss product is one or more of a shampoo product, a hair care product, and an anti-hair loss and hair growth essence.

[0014] The beneficial effects of the present invention are:

[0015] The present invention provides a composition with the efficacy of preventing hair loss and promoting hair growth, specifically comprising ginsenoside CK and biochanin A. The present invention uses ginsenoside CK and biochanin A in combination, and the combination of the two mainly promotes hair growth by enhancing the antioxidant capacity of mouse skin tissue. Data show that ginsenoside CK and biochanin A can synergistically improve the antioxidant capacity of mouse skin tissue within a certain ratio range, and ginsenoside CK and biochanin A can synergistically promote hair growth within a certain ratio range. DETAILED DESCRIPTION

[0016] The present invention provides a composition and application for preventing hair loss and promoting hair growth. To clarify the objectives, technical solutions, and effects of the present invention, the present invention is described in further detail below. It should be understood that the specific embodiments described herein are intended only to illustrate the present invention and are not intended to limit the present invention.

[0017] The embodiment of the present invention provides a composition having the efficacy of preventing hair loss and promoting hair growth, comprising ginsenoside CK and biochanin A.

[0018] In one embodiment, based on weight, ginsenoside CK is 1 part and biochanin A is 0.5-3 parts.

[0019] In this specification, ginsenoside CK refers to the compound described in the following Chemical Formula 1.

[0020]

Chemical Formula 1

[0021]

[0022] The above-mentioned ginsenoside CK is a known compound and can be prepared by methods known in the art. For example, the diol ginsenosides can be separated and purified from Panax notoginseng, ginseng, etc., and then the diol ginsenosides can be converted into rare ginsenoside CK by snail enzyme enzymatic hydrolysis.

[0023] In this specification, biochanin A refers to the compound described in the following Chemical Formula 2.

[0024]

Chemical Formula 2

[0025]

[0026] The biochanin A is a known compound and can be prepared by methods known in the art. For example, biochanin A can be prepared by isolating and purifying isoflavones from clover, chickpea seeds, etc.

[0027] The present invention combines ginsenoside CK and biochanin A within the specified ratios, fully leveraging the hair loss and / or hair growth effects of each component. Furthermore, the combination of ginsenoside CK and biochanin A exhibits synergistic efficacy (CI value less than 1) in treating hair loss and / or hair growth, while also being highly safe. If the ginsenoside CK and biochanin A content of the composition is outside the specified ratios, the synergistic effect is insignificant.

[0028] The purity of ginsenoside CK and biochanin A can be determined by HPLC. The purity of ginsenoside CK can be above 98%, and the purity of biochanin A can be above 98%.

[0029] During application, the composition disclosed in the present invention may contain common auxiliary materials of external cleaning products, for example, auxiliary materials of cleaning products known in the art, such as foaming agents, surfactants, emulsifiers, thickeners, preservatives, fragrances, etc.

[0030] As foaming agents, for example, sodium laureth sulfate, cocoyl monoethanolamide, sodium tridecyl polyether sulfate, sodium lauroyl sarcosinate, cocoyl propyl betaine, etc. can be used; as surfactants, for example, laureth sulfate, lauryl alcohol ether sulfate, cocamidopropyl betaine, cocamide diethanolamine, cocoyl monoethanolamide, etc. can be used; as emulsifiers, PEG-40 hydrogenated castor oil, cetearyl olivate, sorbitan olivate, PEG-4 polyglyceryl-2 stearate, polysorbate 80, etc. can be used; as thickeners, carbomers, xanthan gum, gum arabic, hydroxyethyl cellulose, hydroxypropyl cellulose, and sodium hydroxypropyl cellulose, etc. can be used; as preservatives, potassium sorbate, sodium benzoate, ethylparaben, phenoxyethanol, benzyl alcohol, etc. can be used.

[0031] During the application of the composition disclosed in the present invention, there is no special limitation on the dosage form of the composition, for example, it can be in the form of shampoo, conditioner, anti-hair loss and hair growth essence, etc.

[0032] The following examples are provided to facilitate understanding of the present invention and are not intended to limit the scope of the claims of the present invention in any way.

[0033] Example

[0034] Hair Removal and Modeling: 250 healthy, SPF-free, 8-week-old C57BL / 6J male mice (weighing 18-23 g) were purchased from the Experimental Animal Center, School of Medicine, Xi'an Jiaotong University. After 7 days of adaptive feeding, the hair on the back of the mice was shaved short using a pet shaver over an area of ​​approximately 2 cm × 4 cm. An equal amount of depilatory cream was evenly applied. After approximately 5 minutes, the cream was wiped off with a wet cotton ball. The skin of the mice after depilation was pink, smooth, without ulceration or melanin. The pink color of the mouse skin indicates that the hair follicles are in the resting phase. The day of depilation was designated as day 0, and the day after depilation was designated as day 1.

[0035] Grouping and administration: Mice in the resting phase of hair follicles were randomly divided into a negative control group, a model group, a ginsenoside CK group, a biochanin A group (including 0.1%, 0.2%, 0.4%, and 0.6% biochanin A, respectively), and a ginsenoside CK & biochanin A combination group. The ginsenoside CK: biochanin A weight ratio of 1:0.5, 1:1, 1:2, and 1:3 was mixed uniformly to prepare a certain concentration of composition, and the solvent was 1,2-propylene glycol. They were referred to as composition 1 (0.2% ginsenoside CK + 0.1% biochanin A), composition 2 (0.2% ginsenoside CK + 0.2% biochanin A), composition 3 (0.2% ginsenoside CK + 0.4% biochanin A), composition 4 (0.2% ginsenoside CK + 0.6% Biochanin A) was administered to mice in each group of 10 mice. Starting from the first day of hair removal, all experimental and model groups, except the negative control group, were smeared with an equal volume of testosterone propionate solution on the depilated skin on the back of the mice to establish a model. The dose was 5 mg / kg·day for a total of 28 days. Simultaneously with model establishment, the biochanin A group and the experimental combination groups were topically administered with 5 mL / kg·day of the test substance. The negative control and model groups were smeared with the same volume of normal saline once daily for 28 days.

[0036] Instruments and reagents: Testosterone propionate injection (Ningbo Second Hormone Factory), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) detection kits were purchased from Nanjing Jiancheng Bioengineering Institute.

[0037] Observation and recording of indicators: The change from pink to gray in the skin of mice in each group was considered the time point of the transition from the resting phase to the anagen phase of the hair follicles. The time point when more than 50% of the mice in each group entered the anagen phase was considered the time point of the transition from the resting phase to the anagen phase of the hair follicles. The shortening rate of the telogen phase, the length of the newly grown hair, and the hair coverage rate were statistically analyzed.

[0038] The calculation method of hair follicle telogen shortening rate is as follows: (telogen days of negative control group - telogen days of animals in each group) / telogen days of negative control group × 100%.

[0039] Statistical analysis of new hair length: On day 28, hair was randomly plucked from three locations in each group of newly growing hair mice, and the lengths of 10 hairs were statistically analyzed.

[0040] Statistical analysis method for hair coverage: on day 28, the area of ​​newly grown hair in mice / the area of ​​hair loss area × 100%.

[0041] Index detection: After the completion of the hair growth experiment, mice were killed by cervical dislocation, and the skin of the depilated area on the back was homogenized using radioimmunoprecipitation buffer. The supernatant was centrifuged at 4°C and the supernatant was used for SOD, GSH-Px, and CAT detection according to the instructions provided by the kit, and calibrated with protein concentration.

[0042] The method for calculating the drug combination index CI of the above-mentioned ginsenoside CK / biochanin A combination is as follows:

[0043] The drug combination index (CI) for the ginsenoside CK / biochanin A combination was calculated as (A × B) / AB, where T represents the control group's test result and C represents the test results for each experimental group. For example, A and B represent the C / T values ​​for ginsenoside CK and different doses of biochanin A alone, and AB represents the C / T values ​​for the combined use of ginsenoside CK and biochanin A. A CI < 1 indicates a synergistic effect.

[0044] The effects of ginsenoside CK, biochanin A and compositions 1-4 on the shortening rate of the resting phase of hair follicles in mice are shown in Table 1.

[0045] Table 1 Effects of ginsenoside CK, biochanin A and their combination on the shortening rate of the resting phase of hair follicles in mice

[0046]

[0047] As shown in Table 1, compared with ginsenoside CK and biochanin A at corresponding doses, the CI values ​​for Compositions 2 and 3 were less than 1. However, the CI values ​​for Compositions 1 and 4 were both greater than 1, indicating that ginsenoside CK and biochanin A can synergistically shorten the resting phase of mouse hair follicles within a certain ratio. However, no synergistic effect was observed outside the present invention.

[0048] The effects of the above-mentioned ginsenoside CK, biochanin A and compositions 1-4 on the length of new hair and hair coverage in mice are shown in Table 2.

[0049] Table 2 Effects of various compositions on mouse hair coverage

[0050]

[0051] As shown in Table 2, in terms of hair coverage, the CI values ​​for Compositions 2 and 3 were less than 1 compared to the ginsenoside CK and biochanin A groups at corresponding doses acting alone. However, the CI values ​​for Compositions 1 and 4 were both greater than 1, indicating that ginsenoside CK and biochanin A can synergistically improve hair coverage within a certain ratio. However, no synergistic effect was observed outside the present invention.

[0052] As shown in Table 2, in terms of new hair length, the CI values ​​for Compositions 1, 2, and 3 were all less than 1 compared to ginsenoside CK and the corresponding dose of biochanin A alone. However, the CI value for Composition 4 was greater than 1, indicating that ginsenoside CK and biochanin A can synergistically increase hair coverage within a certain ratio. However, no synergistic effect was observed outside the present invention.

[0053] The effects of the above-mentioned ginsenoside CK, biochanin A and compositions 1-4 on the antioxidant capacity of mouse skin tissue are shown in Table 3.

[0054] Table 3 Effects of various compositions on the antioxidant capacity of mouse skin tissue

[0055]

[0056] SOD, CAT, and GSH-Px are the main components of the enzymatic antioxidant system in tissue cells. These enzymes coordinate with each other to maintain the dynamic balance of the body's oxidation-antioxidation effects, thereby ensuring the body's normal life activities. Enhancing the activity of these three antioxidant enzymes helps maintain redox homeostasis to prevent hair follicle miniaturization.

[0057] As the first line of defense in the body's antioxidant system, SOD specifically scavenges superoxide anion free radicals produced during oxidative metabolism. As shown in Table 3, compared to ginsenoside CK and corresponding doses of biochanin A alone, the SOD drug combination index (CI) values ​​for Compositions 2 and 3 were less than 1. However, the CI values ​​for Compositions 1 and 4 were both greater than 1. This suggests that ginsenoside CK and biochanin A, within a certain ratio range, can synergistically increase SOD activity in mouse skin tissue, thereby enhancing the antioxidant capacity of mouse skin tissue.

[0058] CAT is an enzyme based on an iron porphyrin as a cofactor. It promotes the decomposition of H₂O₂ into molecular oxygen and water, thereby protecting cells from H₂O₂ toxicity. It is a key enzyme in the biological defense system. As shown in Table 3, compared with ginsenoside CK and corresponding doses of biochanin A alone, the CAT drug combination index (CI) values ​​for Compositions 1, 2, and 3 were all less than 1. However, the CI value for Composition 4 was greater than 1. This indicates that ginsenoside CK and biochanin A, within a certain ratio range, can synergistically enhance CAT activity in mouse skin tissue, thereby promoting the decomposition of H₂O₂ and alleviating oxidative stress in tissue cells.

[0059] GSH-Px can specifically catalyze the redox reaction between reduced glutathione and peroxides in the body, while preventing macromolecules from undergoing oxidative stress reactions, converting reduced glutathione into oxidized glutathione, reducing peroxides that are harmful to the body into non-toxic hydroxyl compounds, and decomposing lipid hydroperoxides such as H2O2 into harmless H2O; at the same time, it catalyzes the reduction reaction of organic peroxides formed by unsaturated fatty acids, destroying all epoxides in the body and protecting tissue cells from damage.

[0060] As shown in Table 3, compared with ginsenoside CK and biochanin A at corresponding doses alone, Compositions 2 and 3 exhibited GSH-Px drug combination index (CI) values ​​less than 1. However, Compositions 1 and 4 exhibited CI values ​​greater than 1, indicating that ginsenoside CK and biochanin A, within a certain ratio range, synergistically enhanced GSH-Px activity in mouse skin tissue and alleviated oxidative stress in tissue cells. Overall, Compositions 2 and 3 exhibited a synergistic effect on the activities of SOD, CAT, and GSH-Px.

[0061] In summary, the combination of ginsenoside CK and biochanin A in the present invention promotes hair growth mainly by shortening the resting phase of mouse hair follicles and enhancing the antioxidant capacity of mouse skin tissue. The optimal ratio of ginsenoside CK and biochanin A is 1:1~2.

[0062] It should be noted that, provided that it is feasible and does not significantly violate the main purpose of the present invention, any technical feature or combination of technical features described in this specification as a component of a technical solution may also be applied to other technical solutions; and, provided that it is feasible and does not significantly violate the main purpose of the present invention, technical features described as components of different technical solutions may also be combined in any manner to form other technical solutions. The present invention also includes technical solutions obtained through the above-mentioned combinations, and these technical solutions are equivalent to those described in this specification.

[0063] The present invention has been described above through specific implementation methods and examples, but those skilled in the art should understand that these are not intended to limit the scope of the present invention, and the scope of the present invention should be determined by the claims.

Claims

1. A composition having the efficacy of preventing hair loss and promoting hair growth, characterized in that: Including ginsenoside CK and biochanin A; The weight ratio of ginsenoside CK to biochanin A is 1:1-2.

2. A composition having the efficacy of preventing hair loss and promoting hair growth according to claim 1, characterized in that: The composition further comprises external cleaning product auxiliary materials, which are one or more combinations of foaming agents, emulsifiers, thickeners, preservatives, and fragrances.

3. Use of the composition having the efficacy of preventing hair loss and promoting hair growth according to claim 1 in the preparation of an anti-hair loss product, characterized in that: The anti-hair loss product is a shampoo product or a hair care product.

Images