A kind of Jinju Wuhua Tea Granules and its preparation method

Abstract

The present invention relates to the technical field of traditional Chinese medicine, and discloses a Jinju Wuhua Tea Granule and a preparation method thereof. The Jinju Wuhua Tea is formed by mixing and granulating with clear paste, glycyrrhiza extract coating powder and sucrose as the main components. The clear paste is formed by extracting the medicinal components from honeysuckle flower, kapok flower, kudzu flower, wild chrysanthemum flower and sophora flower, and then concentrating. The addition of the glycyrrhiza extract coating powder can achieve the effect of releasing the glycyrrhiza extract at the target site of oral ulcers. Utilizing the high efficacy of the glycyrrhiza extract against oral ulcers, and cooperating with the balance and anti-inflammatory effect of the polymer outer coating agent on the oral microbial environment, it can effectively achieve the effective treatment of oral ulcers, shorten the illness time of patients with oral ulcers, and reduce the pain of patients.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and particularly relates to a Jinju Wuhua Tea Granule and a preparation method thereof. Background Art

[0002] With the acceleration of the pace of life and the increase in work pressure, many people gradually neglect the importance of daily diet balance and oral hygiene under long-term pressure, which provides a breeding ground for the occurrence of oral ulcers. Moreover, the relatively high work pressure will also cause great fluctuations in emotions, leading to excessive liver fire, commonly known as "getting angry", indirectly increasing the prevalence of oral ulcers. Therefore, in today's society, oral ulcers have gradually become a common problem.

[0003] The early manifestations of oral ulcers are mostly mucosal edema, followed by the formation of intraepithelial blisters, which then gradually dissolve, the mucosa breaks down, and ulcerative plaques are formed. Moreover, a depression will be formed at the ulcer site, and exudate will be produced on the surface of this depression, forming a white or yellow pseudomembrane, which generally heals on its own within 1-2 weeks. When an oral ulcer occurs, inflammation will also occur, accompanied by intense pain and a burning sensation. In severe cases, it will even affect speech and diet, causing great inconvenience to the daily life of the affected person. Currently, the general treatment for oral ulcers is to spray watermelon frost or take Dufemine buccal tablets, etc. However, the residence time of these drugs in the oral cavity is limited, and they gradually lose their efficacy as saliva is swallowed, making it difficult to maintain the drug effect for a long time. Therefore, the treatment effect is not satisfactory.

[0004] The patent application number of the invention patent CN202210598861.9 discloses a sustained-release oral ulcer hydrogel patch, which achieves a sustained-release effect by loading hyperbranched polylysine with acrylic acrylamide hydrogel and Schiff base hydrogel, and produces significant antibacterial, anti-inflammatory and analgesic effects. However, the retention of the gel in the oral cavity may cause discomfort and is not suitable for all affected populations.

[0005] Based on this, the present invention provides a Jinju Wuhua Tea Granule, which can be retained in the oral cavity for a long time by coating the active ingredients therein, and the active ingredients are slowly released, improving the drug utilization rate and having a good therapeutic effect on oral ulcers. Summary of the Invention

[0006] The purpose of the present invention is to provide a Jinju Wuhua Tea Granule and a preparation method thereof, which solve the problem that the conventional drugs for treating oral ulcers are difficult to retain in the oral cavity and have poor treatment effects.

[0007] The purpose of the present invention can be achieved by the following technical solutions:

[0008] A golden chrysanthemum five-flower tea granule, comprising the following components by weight: 10-20 parts of clear paste, 0.5-1.5 parts of liquorice extract coating powder, and 30-45 parts of sucrose;

[0009] The clear paste is prepared by the following method:

[0010] Step 1: Prepare the precursor solution

[0011] Mix honeysuckle, kapok, kudzu flower, wild chrysanthemum and sophora japonica, add to water, decoct for 1-2 hours, pour out the decoction, add water to the residue and decoct again for 0.5-1.5 hours. After the decoction is completed, pour out the decoction, combine the decoctions obtained from the two decoctions, filter, remove the filter residue, and concentrate the filtrate to a relative density of 1.14-1.16 g / mL to form a precursor solution;

[0012] Step 2: Make the clear paste

[0013] The precursor liquid is mixed with 70% ethanol by volume, stirred evenly, and allowed to stand for 12-24 hours, and the supernatant is taken and concentrated to a relative density of 1.28-1.30 g / mL to obtain a clear paste;

[0014] In the first step, the mass ratio of honeysuckle, kapok, kudzu flower, wild chrysanthemum and sophora japonica flower is 1-2:8-15:1.5-2.5:1-2:3.5-4.5;

[0015] The licorice extract coating powder is prepared by coating the licorice extract with a polymer outer coating agent.

[0016] In the above technical scheme, a clear paste is formed by extracting effective ingredients from honeysuckle, kapok, kudzu flower, wild chrysanthemum and sophora flower, which is used as the main ingredient of Jinju Wuhua Tea granules. Among them, honeysuckle enters the lung, stomach and large intestine meridians, and is good at dispersing wind-heat. Kapok has the effects of clearing away heat and detoxifying, relieving depression and relieving restlessness, clearing heat and removing dampness; kudzu flower is sweet and flat in nature, and has the effects of sobering up, invigorating the spleen, and relieving boredom; wild chrysanthemum is a perennial herb of the Asteraceae family, which has the effects of dispersing wind-heat, reducing swelling and detoxifying; sophora flower is the dried buds or opened flowers of the deciduous tree sophora japonica of the Leguminosae family, which has anti-inflammatory, antiviral and other effects; by combining the effective ingredients in honeysuckle, kapok, kudzu flower, wild chrysanthemum and sophora flower, the effects of reducing liver fire, removing lung fire, and relieving boredom are achieved, thereby reducing the incidence of oral ulcers from the inside and reducing the onset period of oral ulcers.

[0017] Furthermore, the polymer outer coating agent is prepared by the following method:

[0018] Step A: Preparation of Zein Pre-solution

[0019] Mix quaternized zein with ethanol, stir evenly, and prepare a zein pre-preparation with a volume fraction of 10-15%;

[0020] Step B. Prepare a sodium oleate pre-preparation

[0021] Add oleic acid to a sodium hydroxide solution with a volume fraction of 20-30%, stir and mix evenly to prepare a sodium oleate pre-preparation with a concentration of 1-3 mol / L;

[0022] Step C. Polymer outer coating agent

[0023] Under continuous stirring conditions, add the zein pre-preparation to the sodium oleate pre-preparation. After mixing evenly, adjust the pH of the solution to 7-8, and stir mechanically for 30-60 minutes to obtain the polymer outer coating agent.

[0024] In the above technical solution, the quaternized zein structure contains quaternary ammonium nitrogen cations, and the long fatty chain after the ionization of sodium oleate carries negative charges, which can attract each other through electrostatic interaction and undergo electrostatic assembly in the solution to form a polymer outer coating agent with an emulsified vesicular shape.

[0025] Furthermore, in step A, the quaternized zein is prepared by the following method:

[0026] Mix zein with dimethyl sulfoxide, stir for 20-40 minutes to form a uniform mixture, then add 2,3-epoxypropyltrimethylammonium chloride to the mixture, protect the whole process with nitrogen, and stir at 60-70 °C for 4-6 hours. Then separate the material, and after dialysis and freeze-drying treatment, quaternized zein can be prepared.

[0027] In the above technical solution, the zein structure contains amino groups, which can undergo ring-opening addition with the epoxy groups in the structure of 2,3-epoxypropyltrimethylammonium chloride, thereby introducing quaternary ammonium salt nitrogen cations into the zein structure to prepare quaternized zein.

[0028] Furthermore, the weight average molecular weight of the zein is 22KDa-26KDa.

[0029] Furthermore, in step A, the volume fraction of the ethanol is 70-80%.

[0030] Furthermore, in step C, the volume ratio of the zein pre-preparation to the sodium oleate pre-preparation is 1:1.

[0031] Furthermore, the licorice extract is prepared by the following method:

[0032] Grind licorice into powder, sieve it through a 80-100 mesh sieve, pour it into an ethanol solution with a volume fraction of 50%, soak it for 12-16h, then raise the temperature to 70-80°C, extract for 2-4h, pour out the extract, mix the residue with ethanol with a volume fraction of 50% again, extract at 70-80°C for 2-3h, filter out the filtrate, combine the filtrates obtained from the two extractions, concentrate under reduced pressure, cool down and discharge, and then freeze-dry to obtain licorice extract.

[0033] Furthermore, the coated powder of licorice extract is prepared by the following method:

[0034] Mix the licorice extract with a high molecular weight outer coating agent, stir and dissolve it, place it in a constant temperature shaking table, shake it at a rate of 60-80r / min, separate the material after 6-8h, and perform freeze-drying treatment to form the coated powder of licorice extract.

[0035] In the above technical solution, after the licorice extract is dissolved in the high molecular weight outer coating agent, it will be wrapped by the high molecular weight outer coating agent under continuous shaking conditions. After the high molecular weight outer coating agent is loaded with a large amount of licorice extract, through drying treatment, the high molecular weight outer coating agent will be wrapped on the surface of the licorice extract to form a coating film, and finally through freeze-drying treatment, the coated powder of licorice extract can be formed.

[0036] Furthermore, the solid-liquid ratio of the licorice extract to the high molecular weight outer coating agent is 1:35-40.

[0037] A preparation method of Jinju Wuhua tea granules includes the following steps:

[0038] Step S1: Weigh each raw material according to parts by weight and set aside;

[0039] Step S2: Mix the clear paste, the coated powder of licorice extract, and sucrose, set the stirring rate to 80-100r / min, stir and mix evenly, make granules, perform drying treatment, and sieve through a 40-60 mesh sieve to obtain Jinju Wuhua tea granules.

[0040] The beneficial effects of the present invention:

[0041] 1) First, the present invention quaternizes zein, which, on the one hand, endows it with good antibacterial properties. By balancing the oral environment, it can inhibit the growth of bacteria and promote the wound healing at the ulcer site in the oral cavity. After electrostatic interaction with the hydrophobic fatty chain of oleic acid, the prepared polymer outer coating agent shows the characteristics of hydrophilic inside and hydrophobic outside. Since there are lipid components in oral mucosal epithelial cells, after the Jinju Wuhua Tea Granules enter the mouth, the glycyrrhiza extract coating powder therein can adsorb on the surface of the oral mucosal wall, and then can directly act on the target site of oral ulcer. Due to the good bioadhesion of zein, the glycyrrhiza extract coating powder in the Jinju Wuhua Tea can adhere to the lesion target site for a long time. With the secretion of saliva, the hydrophilic inside characteristic enables the gradual release of the glycyrrhiza extract. First, components such as glycyrrhizic acid in the glycyrrhiza extract can inhibit the generation of inflammation and relieve pain. Second, the glycyrrhiza extract can inhibit lipid peroxidation, reduce cell damage, and promote cell proliferation, thus promoting the repair of oral mucosa and reducing the duration of the patient's oral ulcer. In addition, oleic acid also has good anti-inflammatory effects, which can further enhance the therapeutic effect of oral ulcer.

[0042] 2) By using a polymer outer coating agent to coat the dry extract, the present invention can, on the one hand, achieve the sustained-release effect of the glycyrrhiza extract and improve the utilization rate of the drug, and on the other hand, also realize the treatment effect on the target site of the oral ulcer lesion, shorten the duration of the disease, and reduce the pain of the patient. In addition, the components in the Jinju Wuhua Tea of the present invention have complementary effects and can prevent and treat oral ulcer from multiple angles from the inside out.

[0043] Of course, it is not necessary for any product implementing the present invention to achieve all the above-mentioned advantages simultaneously. Specific Embodiments

[0044] Next, the technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0045] In the following embodiments, the polymer outer coating agent is prepared by the following method:

[0046] Step A: Prepare quaternized zein

[0047] 2.5 g of zein with a weight average molecular weight of 22 KDa was mixed with dimethyl sulfoxide and stirred for 30 minutes to form a uniform mixed solution. Then 0.6 g of 2,3-epoxypropyltrimethylammonium chloride was added to the mixed solution. Nitrogen was used for protection throughout the process. After stirring at 65°C for 6 hours, the material was separated, dialyzed, and freeze-dried to obtain quaternary ammonium zein.

[0048] Step B: Preparation of Zein Pre-solution

[0049] The quaternized zein is mixed with 80% by volume ethanol, and stirred evenly to prepare a zein pre-liquid with a volume fraction of 15%;

[0050] Step C: Preparation of sodium oleate pre-solution

[0051] Add oleic acid to a 30% by volume sodium hydroxide solution, stir and mix evenly to prepare a sodium oleate pre-solution with a concentration of 2 mol / L;

[0052] Step D, polymer outer coating agent

[0053] Under constant stirring, 100 mL of zein pre-liquid was added to 100 mL of sodium oleate pre-liquid. After mixing evenly, the pH of the solution was adjusted to 8. After mechanical stirring for 40 minutes, a polymer outer coating agent was obtained.

[0054] Example 1

[0055] A golden chrysanthemum five-flower tea granule, comprising the following components by weight: 10 parts of clear paste, 0.5 parts of liquorice extract coating powder, and 30 parts of sucrose;

[0056] The preparation method of the golden chrysanthemum five-flower tea granules comprises the following steps:

[0057] Step S1, weighing each raw material according to weight and setting aside;

[0058] Step S2, mix the clear paste, licorice extract coating powder and sucrose, set the stirring rate to 80r / min, stir and mix evenly, make granules, dry them, and pass them through a 50-mesh sieve to obtain the golden chrysanthemum five-flower tea granules.

[0059] The preparation method of the clear paste comprises the following steps:

[0060] Step 1: Prepare the precursor solution

[0061] Mix 15 g of honeysuckle flower, 120 g of kapok flower, 22.5 g of kudzu flower, 15 g of wild chrysanthemum flower, and 55 g of sophora flower, add to water, and decoct. Control the decocting time to be 1.5 h, pour out the decoction liquid. Add water to the medicinal residues again for decocting, control the decocting time to be 1 h. After completion of decocting, pour out the decoction liquid. Combine the decoction liquids obtained from the two decoctions, filter to remove the filter residues, and concentrate the filtrate to a relative density of 1.14 g / mL to form a precursor liquid.

[0062] Step 2: Prepare clear paste

[0063] Mix 20 mL of the precursor liquid with 120 mL of ethanol with a volume fraction of 70%, stir evenly, let stand for 16 h, take the supernatant, and concentrate to a relative density of 1.30 g / mL to obtain the clear paste.

[0064] The glycyrrhiza extract coating powder is prepared by the following method:

[0065] Step 1: Grind and pulverize glycyrrhiza, pass through a 100-mesh sieve, pour into an ethanol solution with a volume fraction of 50%, soak for 12 h, raise the temperature to 70 °C, extract for 2 h, pour out the extract. Mix the filter residues with ethanol with a volume fraction of 50% again, extract at 70 °C for 2 h, filter out the filtrate, combine the filtrates obtained from the two extractions, concentrate under reduced pressure, cool and discharge, and freeze-dry to obtain the glycyrrhiza extract.

[0066] Step 2: Mix 5 g of the glycyrrhiza extract with 180 mL of a high-molecular outer coating agent, stir to dissolve, place in a constant-temperature shaking incubator, shake at a rate of 60 r / min, separate the material after 6 h, and perform freeze-drying treatment to form the glycyrrhiza extract coating powder.

[0067] Example 2

[0068] A kind of golden chrysanthemum and five-flower tea granules, by weight, comprises the following components: 15 parts of clear paste, 1.2 parts of glycyrrhiza extract coating powder, and 40 parts of sucrose;

[0069] The preparation method of the golden chrysanthemum and five-flower tea granules comprises the following steps:

[0070] Step S1: Weigh each raw material according to the weight parts and set aside;

[0071] Step S2: Mix the clear paste, glycyrrhiza extract coating powder, and sucrose, set the stirring rate to 100 r / min, stir and mix evenly, make into granules, and after drying treatment, pass through a 50-mesh sieve to obtain the golden chrysanthemum and five-flower tea granules.

[0072] The preparation method of the clear paste comprises the following steps:

[0073] Step 1: Prepare precursor liquid

[0074] Mix 15 g of honeysuckle flower, 135 g of kapok flower, 30 g of kudzu flower, 15 g of wild chrysanthemum flower, and 60 g of pagoda tree flower, add water, and decoct. Control the decocting time to be 1.5 h, pour out the decoction liquid, add water to the medicinal residues for decocting again, control the decocting time to be 1 h. After the decocting is completed, pour out the decoction liquid, combine the decoction liquids obtained from the two decoctions, filter to remove the filter residues, and concentrate the filtrate to a relative density of 1.14 g / mL to form a precursor solution.

[0075] Step 2: Prepare the clear extract

[0076] Mix 20 mL of the precursor solution with 120 mL of ethanol with a volume fraction of 70%, stir evenly, let stand for 16 h, take the supernatant, and concentrate it to a relative density of 1.30 g / mL to obtain the clear extract.

[0077] The glycyrrhiza extract coating powder is prepared by the following method:

[0078] Step 1: Grind and pulverize glycyrrhiza, pass through a 100-mesh sieve, pour it into an ethanol solution with a volume fraction of 50%, soak for 16 h, raise the temperature to 80 °C, extract for 3 h, pour out the extract, mix the filter residues with ethanol with a volume fraction of 50% again, extract at 80 °C for 3 h, filter out the filtrate, combine the filtrates obtained from the two extractions, concentrate under reduced pressure, cool and discharge, and freeze-dry to obtain the glycyrrhiza extract.

[0079] Step 2: Mix 5 g of the glycyrrhiza extract with 190 mL of a high-molecular outer coating agent, stir and dissolve, place it in a constant-temperature shaking incubator, shake at a rate of 60 r / min, separate the material after 8 h, and perform freeze-drying treatment to form the glycyrrhiza extract coating powder.

[0080] Example 3

[0081] A kind of golden chrysanthemum and five-flower tea granules, by weight, comprises the following components: 20 parts of clear extract, 1.5 parts of glycyrrhiza extract coating powder, and 45 parts of sucrose;

[0082] The preparation method of the golden chrysanthemum and five-flower tea granules comprises the following steps:

[0083] Step S1: Weigh each raw material according to the weight parts and set aside;

[0084] Step S2: Mix the clear extract, the glycyrrhiza extract coating powder, and sucrose, set the stirring rate to 100 r / min, stir and mix evenly, make into granules, and after drying treatment, pass through a 50-mesh sieve to obtain the golden chrysanthemum and five-flower tea granules.

[0085] The preparation method of the clear extract comprises the following steps:

[0086] Step 1: Prepare the precursor solution

[0087] Mix 15 g of honeysuckle flower, 220 g of kapok flower, 35 g of kudzu flower, 30 g of wild chrysanthemum flower, and 65 g of sophora flower, add to water, and decoct. Control the decocting time to be 2 h, pour out the decoction liquid, add water to the medicinal residues for decocting again, control the decocting time to be 1 h. After completion of decocting, pour out the decoction liquid, combine the decoction liquids obtained from the two decoctions, filter to remove the filter residues, and concentrate the filtrate to a relative density of 1.14 g / mL to form a precursor solution.

[0088] Step 2: Prepare clear paste

[0089] Mix 20 mL of the precursor solution with 120 mL of ethanol with a volume fraction of 70%, stir evenly, let stand for 16 h, take the supernatant, and concentrate it to a relative density of 1.30 g / mL to obtain the clear paste.

[0090] The glycyrrhiza extract coating powder is prepared by the following method:

[0091] Step 1: Grind and crush glycyrrhiza, pass through a 100-mesh sieve, pour into an ethanol solution with a volume fraction of 50%, soak for 16 h, raise the temperature to 80 °C, extract for 4 h, pour out the extract, mix the filter residues with ethanol with a volume fraction of 50% again, extract at 80 °C for 3 h, filter out the filtrate, combine the filtrates obtained from the two extractions, concentrate under reduced pressure, cool and discharge, and freeze-dry to obtain the glycyrrhiza extract.

[0092] Step 2: Mix 5 g of the glycyrrhiza extract with 200 mL of a high-molecular outer coating agent, stir and dissolve, place in a constant-temperature shaking table, shake at a rate of 80 r / min, separate the material after 8 h, and perform freeze-drying treatment to form the glycyrrhiza extract coating powder.

[0093] Comparative Example 1

[0094] A kind of golden chrysanthemum and five-flower tea granules, by weight, comprises the following components: 15 parts of clear paste, 1.2 parts of glycyrrhiza extract, and 40 parts of sucrose;

[0095] The preparation method of the golden chrysanthemum and five-flower tea granules comprises the following steps:

[0096] Step S1: Weigh each raw material according to the parts by weight and set aside;

[0097] Step S2: Mix the clear paste, glycyrrhiza extract, and sucrose, set the stirring rate to 100 r / min, stir and mix evenly, make into granules, and after drying treatment, pass through a 50-mesh sieve to obtain the golden chrysanthemum and five-flower tea granules.

[0098] The preparation method of the clear paste comprises the following steps:

[0099] Step 1: Prepare the precursor solution

[0100] Mix 15 g of honeysuckle, 135 g of kapok, 30 g of kudzu flower, 15 g of wild chrysanthemum, and 60 g of pagoda tree flower, add water, and decoct. Control the decocting time to 1.5 h, pour out the decoction liquid, add water to the medicinal residues for decocting again, control the decocting time to 1 h. After completion of decocting, pour out the decoction liquid, combine the decoction liquids obtained from the two decoctions, filter to remove the filter residues, and concentrate the filtrate to a relative density of 1.14 g / mL to form a precursor solution;

[0101] Step 2: Prepare clear extract

[0102] Mix 20 mL of the precursor solution with 120 mL of ethanol with a volume fraction of 70%, stir evenly, let stand for 16 h, take the supernatant, and concentrate it to a relative density of 1.30 g / mL to obtain the clear extract.

[0103] Among them, the licorice extract is prepared by the following method:

[0104] Step 1: Grind and crush licorice, pass through a 100-mesh sieve, pour it into an ethanol solution with a volume fraction of 50%, soak for 16 h, raise the temperature to 80 °C, extract for 3 h, pour out the extract, mix the filter residues with ethanol with a volume fraction of 50% again, extract at 80 °C for 3 h, filter out the filtrate, combine the filtrates obtained from the two extractions, concentrate under reduced pressure, cool and discharge, and freeze-dry to obtain the licorice extract.

[0105] Experimental example

[0106] Take 50 mice with a body weight of about 30 g, randomly divide them into 5 groups, with 5 males and 5 females in each group, namely the groups of Example 1 - Example 3, the group of Comparative Example 1, and the control group, with 10 mice in each group. Anesthetize all the mice, use a small syringe to suck 0.1 mL of glacial acetic acid with a volume fraction of 40% and inject it into the oral cavity of the mice. Then use a glass rod to evenly apply the glacial acetic acid on the inner wall of the oral cavity of the mice. The next day, it can be seen that obvious deep ulcers appear in the oral cavity of the mice, and the mucosa is congested and edematous. Soak the Jinyin Wuhua tea granules prepared in Example 1 - Example 3 and Comparative Example 1 in water, stir evenly, and prepare a medicinal granule with a volume fraction of 20%. Inject it into the oral cavity of all the mice in the corresponding groups, 0.1 mL each time. The control group is injected with 0.1 mL of distilled water. Raise them under conventional conditions, observe the healing of oral ulcers in each group of mice every day, record the complete healing time of each group of mice, and analyze the data by analysis of variance. The results are recorded in the following table;

[0107] Group Healing time / d Example 1 group <![CDATA[6.7±0.28 ▲ > Example 2 group <![CDATA[6.1±0.46 ▲ <!-- 6 -->]]> Example 3 group <![CDATA[6.6±0.14 ▲ > Comparative example 1 group <![CDATA[10.9±3.16 ▲ > Control group <![CDATA[12.4±2.41 ▲ >

[0108] Note: ▲ Indicates comparison with the blank control group, ▲ P < 0.05.

[0109] Analysis of the test results shows that the Jinju Wuhua Tea granules prepared by mixing the coated licorice extract powder formed by coating the licorice extract with clear paste and other substances can significantly promote the healing of oral ulcers in mice. However, the Jinju Wuhua Tea granules prepared in Comparative Example 1 without coating the licorice extract have a general effect on promoting the healing of oral ulcers. This is because the uncoated licorice extract cannot adhere to the surface of the oral mucosa, and most of the active ingredients directly flow into the stomach, making it difficult to act on the target site of oral ulcers. In addition, it is also impossible to improve the oral microbial environment using the quaternized zein in the polymer outer coating agent, and there may be situations such as bacterial infection, so the treatment effect is poor.

[0110] The above content is only an example and illustration of the concept of the present invention. Those skilled in the art of this technology can make various modifications or supplements to the described specific embodiments or use similar methods for substitution, as long as they do not deviate from the concept of the invention or exceed the scope defined by this claim book, they should all belong to the protection scope of the present invention.

Claims

1. A Jinju Wuhua Tea Granule, characterized in that, It is made of the following components by weight: 10-20 parts of clear extract, 0.5-1.5 parts of glycyrrhiza extract coating powder, and 30-45 parts of sucrose; The clear extract is prepared by the following method: Step 1: Prepare the precursor solution Mix honeysuckle, kapok, kudzu flower, wild chrysanthemum, and pagoda tree flower, add water, and decoct. Control the decocting time to be 1-2 h, pour out the decoction liquid, add water to the medicinal residues again for decocting, control the decocting time to be 0.5-1.5 h. After the decocting is completed, pour out the decoction liquid, combine the decoction liquids obtained from the two decoctions, filter to remove the filter residues, and concentrate the filtrate to a relative density of 1.14-1.16 g / mL to form the precursor solution; Step 2: Prepare the clear extract Mix the precursor solution with ethanol with a volume fraction of 70%, stir evenly, let it stand for 12-24 h, take the supernatant, and concentrate it to a relative density of 1.28-1.30 g / mL to obtain the clear extract; In Step 1, the mass ratio of honeysuckle, kapok, kudzu flower, wild chrysanthemum, and pagoda tree flower is 1-2:8-15:1.5-2.5:1-2:3.5-4.5; The glycyrrhiza extract coating powder is obtained by coating the glycyrrhiza extract with a high-molecular outer coating agent; The high-molecular outer coating agent is prepared by the following method: Step A: Prepare the zein prefabricated solution Mix quaternized zein with ethanol, stir evenly, and prepare a zein prefabricated solution with a volume fraction of 10-15%; Step B: Prepare the sodium oleate prefabricated solution Add oleic acid to a sodium hydroxide solution with a volume fraction of 20-30%, stir and mix evenly, and prepare a sodium oleate prefabricated solution with a concentration of 1-3 mol / L; Step C: High-molecular outer coating agent Under continuous stirring, add the zein prefabricated solution to the sodium oleate prefabricated solution, mix evenly, adjust the pH of the solution to 7-8, and stir mechanically for 30-60 min to obtain the high-molecular outer coating agent; The glycyrrhiza extract is prepared by the following method: Grind and crush licorice, pass through a 80-100 mesh sieve, pour it into an ethanol solution with a volume fraction of 50%, soak for 12-16 h, raise the temperature to 70-80 °C, extract for 2-4 h, pour out the extract, mix the filter residues with ethanol with a volume fraction of 50% again, extract at 70-80 °C for 2-3 h, filter out the filtrate, combine the filtrates obtained from the two extractions, concentrate under reduced pressure, cool and discharge, and freeze-dry to obtain the glycyrrhiza extract; The glycyrrhiza extract coating powder is prepared by the following method: Mix the glycyrrhiza extract with the high-molecular outer coating agent, stir and dissolve, place it in a constant-temperature shaking incubator, shake at a rate of 60-80 r / min, separate the material after 6-8 h, and perform freeze-drying treatment to form the glycyrrhiza extract coating powder.

2. The Herba Chrysanthemi Indici, Flos Lonicerae Japonicae, Folium Artemisiae Argyi, Radix Glycyrrhizae, Radix Scutellariae, and Radix Rehmanniae preparata granule according to claim 1, wherein, In Step A, the quaternized zein is prepared by the following method: Mix zein with dimethyl sulfoxide and stir for 20 - 40 min to form a homogeneous mixture. Then add 2,3-epoxypropyltrimethylammonium chloride to the mixture. Protect the whole process with nitrogen and stir at 60 - 70 °C for 4 - 6 h. After separating the material and performing dialysis and freeze-drying treatments, quaternized zein can be obtained.

3. A kind of Jinju Wuhua Tea Granules according to claim 1, characterized in that, The weight-average molecular weight of the zein is 22KDa - 26KDa.

4. A kind of Jinju Wuhua Tea Granules according to claim 1, characterized in that, In step A, the volume fraction of the ethanol is 70 - 80%.

5. A kind of Jinju Wuhua Tea Granules according to claim 1, characterized in that, In step C, the volume ratio of the zein prefabricated solution to the sodium oleate prefabricated solution is 1:

1.

6. A kind of Jinju Wuhuacha Granules according to claim 1, characterized in that, The solid-liquid ratio of the licorice extract to the high-molecular outer coating agent is 1:35 - 40.

7. A preparation method of the golden chrysanthemum and five-herb tea granules as described in claim 1, characterized in that, It includes the following steps: Step S1: Weigh each raw material according to the weight parts and set aside. Step S2: Mix the clear extract, the licorice extract coating powder, and sucrose, set the stirring rate at 80 - 100 r / min, stir and mix evenly, then make granules. After drying treatment and passing through a 40 - 60 mesh sieve, the Jinju Wuhua Tea granules can be obtained.