A kind of insecticidal composition containing tetrachlorantraniliprole and its use

Abstract

The present invention belongs to the field of insecticides, and relates to an insecticidal composition containing tetrachlorantraniliprole and its use. The composition is an active ingredient A and an active ingredient B in a weight ratio of 1:80-80:1, wherein the active ingredient A is selected from tetrachlorantraniliprole, and the active ingredient B is selected from bromofenac. The insecticidal composition of the present invention has an obvious synergistic effect, can reduce the application amount, and reduce the use cost. The two compounds of the composition of the present invention have different mechanisms of action, and the compounding of the two can alleviate the occurrence and development of insect resistance to a certain extent.

Description

[0001] This application is a divisional application of the Chinese invention patent application. The original application date is December 29, 2021, application number 202111633438.X, the name of the invention: An insecticide composition containing tetrachlorantraniliprole and its use, publication number: CN116406671A; due to the unity problem pointed out by the examiner in the original application, the applicant filed a divisional application. Technical Field

[0002] The invention belongs to the field of insecticides and relates to an insecticide composition containing tetrachlorantraniliprole and a use thereof. Background Art

[0003] Tetrachlorantraniliprole has high activity against lepidopteran pests, low toxicity to mammals, and is safe to non-target organisms such as bees and birds. It is a low-toxic insecticide. Tetrachlorantraniliprole is a nicotinic acid receptor inhibitor and is classified as Group 28 (IRAC Group 28).

[0004] Metadiamides have a broad insecticidal spectrum, good penetration, and are highly active against Lepidoptera, Thysanoptera, and Coleoptera. Current metadiamides include bromofenac, flufenac, isoxadiazole, and cyfluthrin, which are classified as Group 30 (IRAC Group 30) and have no cross-resistance with existing insecticides.

[0005] Although both of the above substances can have a control effect on certain pests, pesticides containing a single active ingredient can easily cause pests to develop resistance if used continuously in pest control. Summary of the invention

[0006] The present invention aims to provide an insecticidal composition containing tetrachlorantraniliprole, which has a synergistic effect, low use cost and good prevention effect, and application thereof.

[0007] The invention discloses an insecticidal composition containing tetrachlorantraniliprole, wherein the composition comprises an active ingredient A and an active ingredient B in a weight ratio of 1:80-80:1, wherein the active ingredient A is selected from tetrachlorantraniliprole, and the active ingredient B is selected from one of the meta-diamide compounds, wherein the meta-diamide compound is one or more of bromofenac, fluoxetine, isoxathiapiprole and cyfluthrin.

[0008] The weight ratio of the active ingredient A to the active ingredient B is 1:15-25:1.

[0009] In the composition,

[0010] The weight ratio of tetrachlorantraniliprole to bromofenac is 1:15-25:1;

[0011] The weight ratio of tetrachlorantraniliprole to fluoxathiapiprole is 1:15-10:1;

[0012] The weight ratio of tetrachlorvinamide to isoxathiapiprolin is 1:15-15:1;

[0013] The weight ratio of tetrachlorantraniliprole to cyfluthrin is 1:15-15:1.

[0014] An application of an insecticidal composition, wherein the composition is used in controlling agricultural and forestry pests, urban pests, and storage pests.

[0015] When the weight ratio of tetrachlorantraniliprole to cyfluthrin in the composition is 15:1-1:5, it is used in preventing and controlling rice borer, striped stem borer and yellow stem borer;

[0016] When the weight ratio of tetrachlorantraniliprole to cyfluthrin in the composition is 1:4-4:1, the composition is used in controlling fall armyworm;

[0017] Application of the composition in preventing and controlling corn borers when the weight ratio of tetrachlorantraniliprole to cyfluthrin is 4:13-15:4;

[0018] When the weight ratio of tetrachlorantraniliprole to cyfluthrin in the composition is 1:3-6:1, the composition can be used in preventing and controlling underground pests such as white grubs, cutworms, small cutworms and wireworms.

[0019] When the weight ratio of tetrachlorantraniliprole to bromofenac is 5:21-10:3, the composition is used for preventing and controlling rice borer, striped stem borer and yellow stem borer;

[0020] When the weight ratio of tetrachlorantraniliprole to bromofenac is 1:4-4:1, the composition is used in controlling fall armyworm;

[0021] When the weight ratio of tetrachlorantraniliprole to bromofenac is 4:15-4:1, the composition is used for preventing and controlling corn borers;

[0022] When the weight ratio of tetrachlorantraniliprole to bromofenac is 1:4-4:1, the composition is used for preventing and controlling cotton aphids, thrips, whiteflies and leafhoppers;

[0023] When the weight ratio of tetrachlorantraniliprole to bromofenac in the composition is 1:8-8:1, the composition can be used in preventing and controlling underground pests such as white grubs, cutworms, small cutworms and wireworms.

[0024] When the weight ratio of tetrachlorantraniliprole to isoxathiapyr in the composition is 5:16-13:4, it is used in preventing and controlling rice borer, striped stem borer and yellow stem borer;

[0025] When the weight ratio of tetrachlorantraniliprole to isoxathiapiprole in the composition is 1:4-4:1, the composition is used in controlling fall armyworm;

[0026] When the weight ratio of tetrachlorantraniliprole to isoxathiapiprole in the composition is 4:15-4:1, it is used for preventing and controlling corn borers;

[0027] When the weight ratio of tetrachlorantraniliprole to isoxathiapiprole in the composition is 1:4-4:1, the composition is used in preventing and controlling Plutella xylostella;

[0028] The composition can be used in preventing and controlling underground pests such as white grubs, cutworms, small cutworms and wireworms when the weight ratio of tetrachlorantraniliprole to isoxathiapiprole is 1:15-15:1.

[0029] The composition is applied to the pests to be controlled or their growth medium at an effective dosage of the composition; wherein the effective dosage is 10 grams to 10,000 grams per hectare.

[0030] The composition of the present invention has the advantages of obvious synergy and expanded control spectrum, and is suitable for controlling a variety of important agricultural and forestry pests, storage pests, and urban pests. The combination can be used for food crops, legume crops, fiber crops, sugar crops, melon crops, fruit crops, dry fruit crops, hobby crops, root crops, oil crops, flower crops, medicinal crops, raw material crops, and green manure and forage crops. The combination can be used to control pests of the order Thysanoptera, and non-exhaustive examples of specific types include but are not limited to rice thrips, tobacco thrips, rice pipe thrips, wheat skin thrips, terminal band thrips, tobacco thrips, flower thrips, yellow thrips, sugarcane thrips, and cucurbit thrips; the combination can be used to control pests of the order Homoptera, and non-exhaustive examples of specific types include but are not limited to greenhouse whitefly, Bemisia tabaci, black-tailed leafhopper, white-winged leafhopper, green leafhopper, cotton leafhopper, Elm leafhopper, small green leafhopper, Indian cotton leafhopper, spotted wax cicada; the combination can be used to control pests of the order Hemiptera, and non-exhaustive examples of specific species include but are not limited to pear web bugs, banana web bugs, fine-horned flower bugs, tiny flower bugs, needle-edged bugs, large rice-edged bugs, rice spider bugs, rice brown bugs, rice black bugs, rice green bugs, green blind bugs, alfalfa blind bugs, middle black blind bugs, wheat root blind bugs, three-spotted blind bugs, pasture blind bugs, litchi bugs; the combination can be used to control pests of the order Lepidoptera, and non-exhaustive examples of specific species include but are not limited to grain moths, clothes moths, yellow thorn moths, brown thorn moths, flat thorn moths, wheat moths, cotton pink bollworms, tobacco budworms, sweet potato wheat moths, diamondback moths, soybean borers, peach borers, hemp borers , pear borer, pear borer, codling moth, apple leaf roller, apple leaf roller, apple transparent wing moth, yellow spotted leaf roller, apple nest moth, brown banded leaf roller, yellow leaf roller, black spotted brown leaf roller, litchi butterfly, striped stem borer, bean pod borer, corn borer, yellow stem borer, Taiwan rice borer, cabbage borer, rice leaf roller, millet ash borer , ash borer, duckweed borer, striped borer, cotton leaf roller borer, sweet potato stem borer, one-spotted grain borer, peach borer, sunflower borer, yellow borer, two-spotted stem borer, white borer, white-banded stem borer, grass borer, armyworm, cutworm, silver-striped armyworm, pit-wing armyworm, quinoa armyworm, jute armyworm, ramie armyworm, mouth kettle armyworm, mango horizontal tail armyworm, cabbage armyworm, beet armyworm, show Noctuidae, loquat yellow caterpillar, ramie red admiral, ramie yellow admiral, rice borer, cotton small bridge borer, large borer, cotton bollworm, cotton leaf roller, soybean leaf roller, peach borer, Dingdian diamond, emerald diamond, Egyptian diamond, small cutworm, large cutworm, yellow cutworm, white-edged cutworm, warning cutworm, thief moth, gypsy moth, sweet potato hawkmoth, bean hawkmoth, straight-striped rice hesperid, hidden-striped valley hesperid, citrus swallowtail, jade-belt swallowtail, cabbage butterfly, double-banded night butterfly, rice leaf night butterfly, powdery moth, tea caterpillar, geometrid, tea moth, large moth, small moth, tea tip moth, tea branch sickle moth, wild silkworm, mulberry caterpillar, tent caterpillar, walnut moth, persimmon calyx worm, citrus leaf miner, banana hesperid;The combination can be used to control pests of the order Coleoptera, and non-exhaustive examples of specific species include, but are not limited to, bean lycopersicon, yellow mealworm, black mealworm, red flour beetle, mixed flour beetle, large flour beetle, saw-shaped flour beetle, long-horned flat flour beetle, black wrinkled gill beetle, clouded gill beetle, yellow-brown beautiful beetle, copper-green beautiful beetle, dark beetle, North China large black gill beetle, mulberry longhorn beetle, star longhorn beetle, orange brown longhorn beetle, peach red neck longhorn beetle, red neck longhorn beetle, sugarcane saw-shaped longhorn beetle, ramie longhorn beetle, hemp longhorn beetle, brown longhorn beetle, light shield green longhorn beetle, big monkey leafworm, small monkey leafworm, rice root leaf beetle, millet scale leaf beetle, hemp leaf beetle, malignant leaf beetle, orange leaf beetle, citrus leaf beetle, yellow melon, yellow curved striped flea beetle, beet flea beetle, grain flea beetle, mung bean weevil, pea weevil, broad bean weevil, corn weevil, rice weevil, root nodule weevil, tea beautiful pattern weevil, tea Seed weevils, beet weevils, rape stem weevils, pear tigers, apricot tigers, peach tigers, black velvet beetles, net-meshed ground beetles, Mongolian ground beetles, rice weevils, rice mudworms, corn iron beetles, sweet potato green beetles, spike-backed glycosidic turtles, light-backed glycosidic turtles, yellow leafworms, banana weevils, banana double black-banded weevils; the combination can be used to control pests of the order Diptera, and non-exhaustive examples of specific species include but are not limited to wheat red midges, wheat yellow midges, rice gall midges, rice chironomids, rice stalk flies, rice leaf miners, rice water flies, citrus fruit flies, citrus fruit flies, citrus fruit flies, melon fruit flies, wheat leaf ash miners, American leaf miners, bean stalk black miners, wheat stalk flies, seed flies, onion flies, radish flies, sorghum awn flies, millet stalk flies, astragalus leaf miners, duckweed chironomids, beet leaf miners, beet root maggots, and small radish flies. ;

[0031] The present invention has the following advantages:

[0032] The insecticide composition of the present invention has an obvious synergistic effect, can reduce the application amount, and reduce the use cost. The two compounds in the composition of the present invention have different action mechanisms, and the combination of the two can alleviate the occurrence and development of insecticide resistance to a certain extent; specifically:

[0033] 1. The composition of the present invention exhibits obvious synergistic effect within a certain ratio range, thereby improving the control effect of the compound on pests; thereby meeting the demand for increasing effectiveness and continuous renewal of insecticide varieties in the control of pests in the fields of agriculture, forestry and urban sanitation.

[0034] 2. The composition of the present invention reduces the dosage of the compound during application, thereby reducing the cost of use and reducing environmental pollution.

[0035] 3. The effective ingredients in the composition of the present invention have different mechanisms of action and do not have the problem of cross-resistance. Using it to control pests can delay the occurrence of pest resistance and improve the control effect on resistant populations. DETAILED DESCRIPTION

[0036] The following specific examples are used to further illustrate the present invention, but the present invention is by no means limited to these examples. The percentage or proportion of each component in the examples is by weight. The active component in each formula is calculated as the effective content, and each substance in the composition can be prepared in a conventional manner.

[0037] Example 1 Determination of the synergistic effect of the combination of tetrachlorantraniliprole and cyprodinil on Chilo suppressalis

[0038] The test was conducted with reference to “Guidelines for Indoor Bioassay Tests of Pesticides-Insecticides Part 7: Determination of Combined Action of Mixtures” (NY / T 1154.7-2006).

[0039] Test target: 3rd instar Chilo suppressalis (Walker), a sensitive strain reared indoors.

[0040] Preparation of drug solution: According to different test needs, the test samples were accurately weighed using an electronic analytical balance. The original drug was dissolved in acetone and then diluted with 0.1% Tween 80 aqueous solution to prepare single-dose stock solutions. More than 5 groups of ratios were designed based on the mixing purpose and the activity of the drug. Five series of mass concentrations were set for each single dose and each group of mixed ratios according to the equal ratio method.

[0041] Test method: Spray method was used. Rice stalks of uniform length and thickness were selected, cut to the same length, and sprayed evenly in the order from low dose to high dose according to the test design. 1.5 mL of the solution was placed in a 6 cm diameter culture dish with filter paper for each treatment. After natural shade drying, neat healthy test insects were inoculated. 15 insects were treated for each treatment, and 4 replicates were performed for each treatment. A blank control was also set.

[0042] The treated test materials were placed in an observation room, where the temperature, humidity and light intensity could be adjusted as needed. The number of dead and live insects was investigated after 72 hours. The corrected mortality rate was calculated using the Abbott formula, and statistical analysis was performed using the DPS data processing software to obtain the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect. The test results are shown in Table 1.

[0043] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0044]

[0045] Where: ATI-actual toxicity index of the mixture; S-LC of the standard agent 50 Value; LC of M-mixture 50 value.

[0046] TTI=TIA×PA+TIB×PB

[0047] Where: TTI-theoretical toxicity index of mixture; TIA-toxicity index of A agent; percentage of PA-A agent in the mixture; TIB-toxicity index of B agent; percentage of PB-B agent in the mixture.

[0048]

[0049] Where: CTC-co-toxicity coefficient; ATI-actual toxicity index of mixture; TTI-theoretical toxicity index of mixture.

[0050] A co-toxicity coefficient higher than 120 is a synergistic effect, CTC.

[0051] Table 1 The results of the combined toxicity test of tetrachlorantraniliprole and cyfluthrin against Chilo suppressalis

[0052] deal with Virulence regression curve (Y=a+bX) <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient (CTC) Tetrachlorantraniliprole (A) Y=3.0368+2.2409X 7.5173 — Ciprofloxacin (B) Y=5.1195+1.8798X 0.8639 — A:B=15:1 Y=4.0895+2.3741X 2.4183 209.8 A:B=11:1 Y=4.7228+2.6356X 1.2740 359.4 A:B=5:1 Y=4.7305+2.8230X 1.2458 264.2 A:B=2:1 Y=5.0176+2.4889X 0.9838 214.2 A:B=3:2 Y=4.8955+2.7434X 1.0917 168.7 A:B=1:1 Y=5.1653+2.6283X 0.8651 179.1 A:B=1:2 Y=5.1565+2.6748X 0.8739 140.2 A:B=1:5 Y=5.5952+2.1182X 0.5236 193.5 A:B=1:11 Y=5.5451+1.8849X 0.5138 181.5 A:B=1:15 Y=5.5541+1.9573X 0.5211 175.5

[0053] Example 2 Determination of the synergistic effect of the combination of tetrachlorantraniliprole and bromofenac on Chilo suppressalis

[0054] The test was conducted with reference to “Guidelines for Indoor Bioassay Tests of Pesticides-Insecticides Part 7: Determination of Combined Action of Mixtures” (NY / T 1154.7-2006).

[0055] The test method is the same as in Example 1. The test results are shown in Table 2.

[0056] Table 2 Results of the combined toxicity test of tetrachlorantraniliprole and bromofenac on Chilo suppressalis

[0057] deal with Virulence regression curve (Y=a+bX) <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient (CTC) Tetrachlorantraniliprole (A) Y=3.0368+2.2409X 7.5173 — Brofenac (B) Y=4.6610+2.0320X 1.4683 — A:B=10:3 Y=4.1161+2.6252X 2.1712 177.5 A:B=2:1 Y=3.9292+3.0685X 2.2333 141.8 A:B=1:1 Y=4.2533+2.6762X 1.9012 129.2 A:B=1:2 Y=4.9223+2.5347X 1.0732 187.0 A:B=1:3 Y=4.9871+2.5045X 1.0119 181.6 A:B=5:21 Y=4.6866+2.6368X 1.3148 132.1

[0058] Example 3 Determination of the synergistic effect of the combination of tetrachlorantraniliprole and cyprodinil on corn borer

[0059] The test was conducted with reference to “Guidelines for Indoor Bioassay Tests of Pesticides-Insecticides Part 7: Determination of Combined Action of Mixtures” (NY / T 1154.7-2006).

[0060] Test subjects: Corn borer Pyrausta nubilalis (Hubern), 3 years old, sensitive strain raised indoors.

[0061] Preparation of drug solution: According to different test needs, the test samples were accurately weighed with an electronic analytical balance, the original drug was dissolved in acetone, and then diluted with 0.1% Tween 80 aqueous solution to prepare single-dose stock solutions. More than 5 groups of ratios were designed according to the mixing purpose and the activity of the drug. Five series of mass concentrations were set for each single dose and each group of mixed ratios according to the equal ratio method.

[0062] Test method: drug film method. Add 1 mL of artificial feed to each hole. After the feed cools down, add the prepared drug solution in the order from low to high according to the test design, shake evenly to make a drug film, dry naturally in the shade, and then inoculate with standard test insects. Inoculate one insect in each hole, 15 insects as one treatment, repeat 4 times, and set a blank control.

[0063] The treated test materials were placed in an observation room, where the temperature, humidity and light intensity could be adjusted as needed. The number of dead and live insects was investigated after 72 hours. The corrected mortality rate was calculated using the Abbott formula, and statistical analysis was performed using the DPS data processing software to obtain the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect. The test results are shown in Table 3.

[0064] Table 3 Results of combined toxicity test of tetrachlorantraniliprole and cyfluthrin against corn borer

[0065] deal with Virulence regression curve (Y=a+bX) <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient (CTC) Tetrachlorantraniliprole (A) Y=5.4348+2.0071X 0.6072 — Ciprofloxacin (B) Y=5.8770+2.7413X 0.4787 — A:B=15:4 Y=5.9215+2.4072X 0.4142 138.8 A:B=3:1 Y=5.7787+1.9959X 0.4072 139.7 A:B=2:1 Y=6.0383+2.2663X 0.3482 160.1 A:B=3:2 Y=6.0955+1.8563X 0.2570 213.4 A:B=4:3 Y=6.0051+2.1964X 0.3487 156.2 A:B=1:1 Y=6.0379+2.2589X 0.3471 154.2 A:B=3:4 Y=5.9113+1.8439X 0.3205 164.3 A:B=2:3 Y=5.8594+2.0997X 0.3897 134.2 A:B=1:2 Y=5.9850+2.2216X 0.3603 142.9 A:B=1:3 Y=6.0974+2.3130X 0.3354 150.7 A:B=4:13 Y=6.0801+2.0463X 0.2966 169.9

[0066] Example 4 Determination of the synergistic effect of the combination of tetrachlorantraniliprole and cyprodinil on fall armyworm

[0067] Refer to "Guidelines for Indoor Bioassay Tests of Pesticides - Pesticides Part 7: Determination of Combined Action of Mixtures" (NY / T1154.7-2006).

[0068] Test subjects: Spodoptera frugiperda, 3rd instar, sensitive strain raised indoors.

[0069] Preparation of drug solution: According to different test needs, the test samples were accurately weighed using an electronic analytical balance. The original drug was dissolved in acetone and then diluted with 0.1% Tween 80 aqueous solution to prepare single-dose stock solutions. More than 5 groups of ratios were designed based on the mixing purpose and the activity of the drug. Five series of mass concentrations were set for each single dose and each group of mixed ratios according to the equal ratio method.

[0070] Test method: Spray method was used. Cut the middle leaves of fresh corn grown in the greenhouse into small pieces of the same length, and spray the prepared liquid medicine evenly on the front and back of the leaves with an airbrush manual sprayer in the order of low dose to high dose according to the test design. Place the leaves in a 6 cm diameter culture dish with filter paper, dry them naturally in the shade, and then inoculate them with neat healthy test insects. Each treatment had 15 heads, repeated 4 times, and a blank control.

[0071] The treated test materials were placed in an observation room, where the temperature, humidity and light intensity could be adjusted as needed. The number of dead and live insects was investigated after 72 hours. The corrected mortality rate was calculated using the Abbott formula, and statistical analysis was performed using the DPS data processing software to obtain the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect. The test results are shown in Table 4.

[0072] Table 4 The results of the combined toxicity test of tetrachlorantraniliprole and cyfluthrin against fall armyworm

[0073] deal with Virulence regression curve (Y=a+bX) <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient (CTC) Tetrachlorantraniliprole (A) Y=4.7643+2.0611X 1.3012 — Ciprofloxacin (B) Y=5.3617+1.8643X 0.6397 — A:B=4:1 Y=5.6230+2.1265X 0.5093 211.7 A:B=3:1 Y=5.6393+2.1762X 0.5084 203.4 A:B=2:1 Y=5.7383+2.2381X 0.4679 206.8 A:B=1:1 Y=5.6009+2.1102X 0.5191 165.2 A:B=1:2 Y=5.6010+2.1265X 0.5216 147.6 A:B=1:3 Y=5.5348+2.0688X 0.5515 132.9 A:B=1:4 Y=5.5064+1.8187X 0.5267 135.2

[0074] Example 5 Determination of the synergistic effect of the combination of tetrachlorantraniliprole and cyprodinil on cutworms

[0075] Refer to "Guidelines for Indoor Bioassay Tests of Pesticides - Pesticides Part 7: Determination of Combined Action of Mixtures" (NY / T1154.7-2006).

[0076] Test subjects: Cutworm (Agrotis ypsilon), 2nd instar larvae, sensitive strain reared indoors.

[0077] Preparation of drug solution: According to different test needs, the test samples were accurately weighed using an electronic analytical balance. The original drug was dissolved in acetone and then diluted with 0.1% Tween 80 aqueous solution to prepare single-dose stock solutions. More than 5 groups of ratios were designed based on the mixing purpose and the activity of the drug. Five series of mass concentrations were set for each single dose and each group of mixed ratios according to the equal ratio method.

[0078] Test method: Spray method was used. Fresh cabbage leaves grown in the greenhouse were punched into leaf discs with a diameter of 4 cm using a hole puncher. The prepared liquid was evenly sprayed on the front and back of the leaves using an airbrush manual sprayer in the order of low dose to high dose according to the test design. The leaves were placed in a 6 cm diameter culture dish with filter paper. After natural drying, neat healthy test insects were inoculated. 15 heads were treated each time, with 4 replicates, and a blank control was used.

[0079] The treated test materials were placed in an observation room, where the temperature, humidity and light intensity could be adjusted as needed. The number of dead and live insects was investigated after 48 hours. The corrected mortality rate was calculated using the Abbott formula, and statistical analysis was performed using the DPS data processing software to obtain the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect. The test results are shown in Table 5.

[0080] Table 5 The results of the combined toxicity test of tetrachlorantraniliprole and cyprodinil on cutworms

[0081] deal with Virulence regression curve (Y=a+bX) <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient (CTC) Tetrachlorantraniliprole (A) Y=6.0631+2.6021X 0.3903 — Ciprofloxacin (B) Y=5.7296+2.3031X 0.4822 — A:B=5:1 Y=6.8800+2.5651X 0.1850 217.9 A:B=4:1 Y=6.8271+2.6892X 0.2092 194.0 A:B=3:1 Y=6.5933+2.3860X 0.2149 190.7 A:B=2:1 Y=6.6426+2.5239X 0.2235 186.5 A:B=1:1 Y=6.5762+2.3435X 0.2125 203.0 A:B=1:2 Y=6.9208+2.7999X 0.2061 216.9 A:B=1:3 Y=6.5884+2.3946X 0.2171 209.8 A:B=1:4 Y=6.5911+2.5042X 0.2316 198.8

[0082] Example 6 Determination of the synergistic effect of the combination of tetrachlorantraniliprole and cyprodinil on grubs

[0083] Refer to "Guidelines for Indoor Bioassay Tests of Pesticides - Pesticides Part 7: Determination of Combined Action of Mixtures" (NY / T1154.7-2006).

[0084] Test subjects: Second-instar larvae of Holotrichia oblita Faldermann, a sensitive strain reared indoors.

[0085] Preparation of drug solution: According to different test needs, the test samples were accurately weighed with an electronic analytical balance, the original drug was dissolved in acetone, and then diluted with 0.1% Tween 80 aqueous solution to prepare single-dose stock solutions. More than 5 groups of ratios were designed according to the mixing purpose and the activity of the drug. Five series of mass concentrations were set for each single dose and each group of mixed ratios according to the equal ratio method.

[0086] Test method: Adopt the dipping method. Select neat and healthy test insects, put them into the dipping cage, immerse for 10 seconds, take out the test insects, dry them naturally in the shade, and place them in a culture dish with a diameter of 6 cm and filter paper. Add potato pieces with a diameter of 2 cm×2 cm×2 cm in the culture dish. Each treatment has 15 insects, repeated 4 times, and set up a blank control.

[0087] The treated test materials were placed in an observation room, where the temperature, humidity and light intensity could be adjusted as needed. The number of dead and live insects was investigated after 72 hours. The corrected mortality rate was calculated using the Abbott formula, and statistical analysis was performed using the DPS data processing software to obtain the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect. The test results are shown in Table 6.

[0088] Table 6 The results of the combined toxicity test of tetrachlorantraniliprole and cyprodinil on grubs

[0089]

[0090]

[0091] Example 7 Determination of the synergistic effect of the combination of tetrachlorantraniliprole and bromofenac on white grubs

[0092] Refer to "Guidelines for Indoor Bioassay Tests of Pesticides - Pesticides Part 7: Determination of Combined Action of Mixtures" (NY / T1154.7-2006).

[0093] The test method is the same as in Example 6. The test results are shown in Table 7.

[0094] Table 7 Results of the combined toxicity test of tetrachlorantraniliprole and bromofenac on grubs

[0095] deal with Virulence regression curve (Y=a+bX) <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient (CTC) Tetrachlorantraniliprole (A) Y=1.7259+2.0972X 36.4083 — Brofenac (B) Y=2.4780+1.9432X 19.8537 — A:B=8:1 Y=2.6158+1.8940X 18.1466 183.6 A:B=4:1 Y=2.6261+1.9787X 15.8407 197.0 A:B=2:1 Y=2.7534+1.9000X 15.2207 187.2 A:B=1:1 Y=2.6011+2.0313X 15.1691 169.4 A:B=1:2 Y=2.4859+2.1437X 14.8877 157.2 A:B=1:4 Y=2.3801+2.2430X 14.7238 148.3 A:B=1:8 Y=1.9888+2.5512X 15.1457 138.1

Claims

1. An insecticidal composition containing tetrachlorantraniliprole, characterized in that: The composition comprises active ingredient A and active ingredient B in a weight ratio of 1:8-8:1, wherein the active ingredient A is selected from tetrachlorantraniliprole, and the active ingredient B is selected from bromofenac.

2. An application of the insecticidal composition containing tetrachlorantraniliprole according to claim 1, characterized in that: The composition is used in controlling striped stem borers and white grubs.

3. The use according to claim 2, characterized in that: When the weight ratio of tetrachlorantraniliprole to bromofenac is 5:21-10:3, the composition is used for preventing and controlling striped stem borer; When the weight ratio of tetrachlorantraniliprole to bromofenac in the composition is 1:8-8:1, it is used in preventing and controlling underground pests such as white grubs.

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