Preparation method of Moringa cutting material and cutting method thereof

Abstract

The present invention discloses a method for preparing Moringa cutting material and a cutting method thereof, relating to the technical field of Moringa cutting propagation. The present invention utilizes micro-cuttings to culture tender shoots from mature, high-quality individual trees with excellent field yields, thereby preparing rooting tender stem bud material for repeated cutting propagation. This method utilizes asexual propagation of high-quality individual trees, enabling better preservation of the excellent traits of the mother plant while simultaneously producing large quantities of seedlings. This method solves the problem of direct cuttings of tender shoots from Moringa mother plants failing to root and form seedlings, enabling rapid production of large quantities of cutting material and the establishment of a large-scale cutting system.

Description

Technical Field

[0001] The invention relates to the technical field of Moringa cutting propagation, and more particularly to a preparation method of Moringa cutting material and a cutting method thereof. Background Art

[0002] Moringa (Moringa oleifera Lam.) is a tree in the genus Moringa, Moringaceae. It boasts numerous applications and is considered the world's most nutritious tree, a treasure trove from head to toe. Native to India, it has been used for thousands of years as a food and medicine, making it one of humanity's oldest trees used for both medicine and food. Known as the "miracle tree," "a mother's best friend," and "a treasure chest of medicine," Moringa is a treasure trove. Its green fruits, resembling long beans, hang naturally from the tree, creating an ornamental sight and making it particularly suitable for sightseeing orchards and leisure farms. The young shoots, leaves, and fruits can be eaten as vegetables, boasting spleen-strengthening and dampness-reducing properties. The seeds can be used to extract oil, a lubricant. They are highly absorbent and stable, making them a fragrance fixative.

[0003] Moringa is mainly propagated by sowing, but the price of seeds is high, and the seedlings propagated by seeds vary greatly from plant to plant, resulting in unstable yields, which to a certain extent limits the healthy development of the moringa industry. As for the cutting propagation method, the tender shoots cut from the mother plant cannot take root and grow into seedlings when directly cut. Corky or semi-corky mature branches are required as cutting materials for survival. However, this method requires a large amount of material and cannot be propagated in large quantities at the same time. In addition, the survival rate is low and cannot meet production needs.

[0004] Therefore, providing a high-efficiency Moringa propagation method is a problem that those skilled in the art are in urgent need of solving. Summary of the Invention

[0005] In view of this, the present invention provides a preparation method of Moringa oleifera cutting material and a cutting method thereof.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A method for preparing Moringa cutting materials, wherein Moringa oleifera shoots are subjected to a micro-cutting asexual propagation method using a culture medium to establish a mother garden to prepare the cutting materials, comprising the following steps:

[0008] S1: Cut the main trunk of the mother plant and wait for the new shoots to grow to 20-30 cm. Cut the tender shoots at the tip as explants.

[0009] S2 explant disinfection pretreatment;

[0010] S3 induction culture: Place the sterilized explants flat in sterile bud induction medium and culture them at a temperature of 24-26°C, a light intensity of 1500-2000 lx, a light intensity of 9 hours / day, and a humidity of 50-70%. Culture them aseptically for 35-40 days to obtain 5-7 cm sterile axillary buds.

[0011] S4 rooting culture: cut the sterile axillary buds into 3 cm long axillary bud segments and insert them into the rooting medium for culture. Control the temperature at 24-26°C, the light intensity at 1500-2000 lx, the light intensity at 9 h / d, and the humidity at 50-70%. Culture the sterile axillary buds for 35-38 days to obtain rooted seedlings.

[0012] S5 seedling hardening and transplanting: Build a moisturizing shed in the greenhouse and transplant the rooted seedlings into the rooting medium in the moisturizing shed. On the 1st to 5th day after transplanting, water once in the morning and evening and control the humidity to 95%. On the 6th day, open the moisturizing shed 1 / 3; on the 6th to 10th day, water once in the evening and control the humidity to 85%; open the moisturizing shed fully for 15 days after transplanting, and then control the humidity to 70%; during this period, control the temperature of the greenhouse at 25-36°C and the shade degree at 70%;

[0013] S6 mother garden is established to obtain cutting materials: 45-50 days after the rooted seedlings are transplanted, the Moringa seedlings grow to a height of 25-35 cm, and the stems are cut and planted in a greenhouse to obtain the mother garden; the new tender shoots of the plants in the mother garden are cut to obtain cutting materials.

[0014] The present invention uses micro-cuttings to culture tender shoots from mature, high-yield trees to produce rooting tender stem buds for repeated cutting propagation. This method, which involves asexual propagation of high-yield individual plants, can better preserve the excellent traits of the mother plant while simultaneously producing large quantities of seedlings. This method solves the problem of direct cuttings of tender shoots from Moringa mother plants failing to root and form seedlings, allowing for rapid production of large quantities of cutting material and the establishment of a large-scale cutting system.

[0015] As a preferred technical solution, the S1 mother plant is a single plant of improved quality that is more than 3 years old. The trunk is cut at a height of 1.2 meters from the ground and is sawn at a 20-degree angle. The saw cut is smooth and wrapped to prevent moisture loss. More preferably, the saw cut is wrapped with three layers of plastic wrap.

[0016] As an optimal technical solution, when S1 cuts the tender buds as explants, 2-3 axillary bud points are retained near the main trunk. New buds can be grown on the reserved bud points of the mother plant 15-20 days after cutting and reused. The cutting time is noon on a sunny day.

[0017] As an optimal technical solution, the S2 explant disinfection pretreatment method is to cut the explant into 4 cm single bud segments, first rinse with tap water for 20 minutes, then wash with a diluted detergent solution (Libai brand, 10g of stock solution to 700ml of water) by hand for 5 minutes, and finally rinse with tap water for 50 minutes, then cut off 0.5 cm of the upper and lower incisions of the single bud segment and the position of the bud at the bifurcation of the stem and leaves, retaining a 3 cm long tender stem segment, and disinfect in the order of alcohol disinfection for 30 seconds - sterile water washing for 3 minutes, repeat once - 2 drops of Tween + 0.1% mercuric chloride for 9 minutes - sterile water washing for 3 minutes, repeat twice.

[0018] As a preferred technical solution, the composition of the induction medium in S3 is MS + IBA 0.6 mg / L + NAA 0.2 mg / L + sucrose 15 g / L + agar 5 g / L, pH 6.5;

[0019] The composition of the rooting medium in S4 is MS + IBA 0.2 mg / L + NAA 0.1 mg / L + sucrose 15 g / L + agar 6 g / L + ripe fresh banana 9 g / L + activated carbon 0.6 g / L, pH 5.7;

[0020] S5 The rooting medium comprises garden soil, river sand and peat soil in a weight ratio of 5:3:2; more preferably, the rooting medium is mixed evenly and then exposed to the sun for 2-3 days.

[0021] As a preferred technical solution, the S5 seedling hardening and transplanting process begins with establishing a seedling bed: select a sunny, well-ventilated location within the greenhouse, avoiding dark and damp conditions. Build the four sides of the seedling bed with materials such as bricks, river sand, and cement. Design the dimensions based on needs and actual conditions. The bed width should not exceed 1.2 meters, and the bed length can be adjusted based on actual conditions. Prepare the bottom of the seedling bed by deep-plowing the soil in this area to 20 cm. This area should be deeply plowed and leveled, and then covered with a 10 cm layer of loose coconut coir to enhance moisture retention and air permeability at the bottom of the nutrient bag.

[0022] As a preferred technical solution, the rooting medium described in S5 is contained in a nutrient bag, and the nutrient bag is placed on a seedbed covered with coconut chaff. In the seedbed, coconut chaff is irrigated with water 2 days before use. The nutrient bag is placed and the seedbed is sprayed with 500 times of wettable powder mancozeb solution on the same day for disinfection. Preferably, the nutrient bag is placed in the seedbed and the upper port is lower than the upper mouth 1-2cm of the seedbed. Watering, transplanting, and management can be better carried out like this, and the later stage bag seedlings are moisturized, and the effect of promoting root growth is improved. After the seedling bag branches are placed on the seedbed, watering with a spray pipe is standby.

[0023] As a preferred technical solution, before S5 seedling hardening and transplanting, the rooting bottle is moved to the indoor natural light hardening rack and the cover is loosened for one day, then the bottle mouth is opened 1 / 4 and kept for 2 days before transplanting into the rooting medium.

[0024] As a preferred technical solution, when cutting and planting as described in S6, the stem is cut at a height of 15 cm, and the resulting tender shoots can also be used as cutting materials; and the cutting materials obtained in S6 are cut into 8 to 10 cm tender stem buds as cuttings according to the standards of long internodes with one bud, short internodes with two buds, and segments with terminal buds.

[0025] As a preferred technical solution, after cutting the stem, select the vigorously growing plants and plant them in rows with a spacing of 20×20 cm to obtain tender shoot cutting materials from the mother garden. Before cutting the stem, the cutting seedlings are relatively slender. After cutting the stem and then planting, the seedlings become thicker and stronger, which is conducive to the survival of the cutting materials.

[0026] Another object of the present invention is to provide a cutting method for propagating Moringa oleifera using the above-mentioned cutting material, comprising the following steps:

[0027] (1) Every time before cutting the cutting material, spray "Amaisu" amino acid water-soluble fertilizer once (spray evenly on the front and back of the stems and leaves), the concentration is 20 ml to 15 liters of water;

[0028] (2) Cuttings were carried out in a greenhouse with a shade of 70%. The cutting medium was watered thoroughly on the second day before cutting. The cutting medium was turned over 7 cm in the afternoon of the day before cutting (using a 25 cm long bamboo stick to turn the soil). Cuttings were carried out in the evening, with a cutting depth of 3-4 cm. Within 30 minutes after cutting, the cuttings were irrigated with 500 times the wettable powder "Mancozeb". The plants were covered with transparent moisturizing bottles (specifications: bottle mouth diameter 5.5 cm × bottle bottom diameter 9 cm × height 13.5 cm, volume 750 ml). After all the plants were planted, a small amount of water was poured to moisten the roots.

[0029] (3) Water and fertilizer management: After cutting, control the temperature at 25-36 degrees. Spray water once in the morning and evening 5 days before cutting, water once in the evening on the 6th to 10th day, and water once every 2-3 days in the later period according to weather conditions. Ensure that the coconut coir in the seedbed and the bottom of the nutrient bag are moist, and control the humidity in the moisturizing bottle to be above 95%; 15-17 days after cutting, remove the moisturizing bottle in the evening (the moisturizing bottle can be recycled continuously), and water it thoroughly after opening the bottle. Control the humidity at 85%-95% on the day, and keep the humidity above 70% in the later period. 60 days after cutting, it can be transplanted to the field for planting.

[0030] As a preferred technical solution, when the cuttings grow to 30-50 cm, you can also take the stem buds with 1 to 2 axillary buds above 20 cm as a bud segment and use it as cutting material again; 5 to 7 days before taking the tender stem buds for cuttings, cut off the petioles and leaves in the middle of each leaf canopy in the stem segment.

[0031] It can be seen from the above technical solutions that compared with the prior art, the present invention has the following beneficial effects:

[0032] 1. Through asexual propagation (micro-cutting) of the tender shoots of the improved mother plants, a mother garden of stem bud cuttings that meets production needs can be quickly established, which provides a guarantee for the direct cutting of stem buds into seedlings in the later stage, and solves the problem that the tender shoots of the Moringa mother plants cannot take root and form seedlings by direct cuttings.

[0033] 2. The cutting material obtained by the method of the present invention has low requirements for the cutting medium and can obtain a good rooting rate in a variety of media.

[0034] 3. It is convenient to use young shoots from the mother garden for cuttings, the seedlings grow quickly, it saves costs, and the seedlings are neatly and uniformly planted.

[0035] 4. After cutting the tender stems and buds of the seedlings in the mother garden, cutting materials can be produced again within 20 days, and cutting propagation can be carried out by cutting stems and buds throughout the year.

[0036] 5. The stem buds that have grown into seedlings through cuttings can be cut into seedlings again. With the mother garden of cuttings as the source of cutting propagation materials, a large cutting system can be quickly established. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are merely embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying any creative work.

[0038] Figure 1-Figure 3 Schematic diagram of tender stem bud cutting material provided by an embodiment of the present invention, wherein: Figure 1 For terminal bud cuttings, Figure 2 For single bud cuttings, Figure 3 It is a two-bud cutting.

[0039] Figure 4 A schematic diagram of moisturizing in a moisturizing bottle after cutting provided by an embodiment of the present invention.

[0040] Figure 5 Schematic diagram of the rooted seedlings of tender stem bud cuttings (22 days after cutting) provided in an embodiment of the present invention.

[0041] Figure 6 A schematic diagram showing that the cutting seedlings provided in the comparative example of the present invention cannot take root (22 days after cutting). DETAILED DESCRIPTION

[0042] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0043] The embodiment of the invention discloses a preparation method of Moringa oleifera cutting material and a cutting method thereof.

[0044] The method of the present invention is applicable to Moringa polyoleifera.

[0045] The cutting tools, fertilizers, etc. involved are all commercially available, such as Yanhua Blue Brand Mancozeb (active ingredient content 80%, dosage form: wettable powder), manufactured by Beijing Yanhua Yongle Biotechnology Co., Ltd., and the "Amaisu" amino acid-containing water-soluble fertilizer for spraying can be purchased from Shenzhen Runkang Ecological Environment Co., Ltd., and no specific requirements are made on its source.

[0046] Example 1

[0047] Step 1, a method for preparing Moringa cutting materials, wherein Moringa oleifera shoots are subjected to a culture medium micro-cutting asexual propagation method to establish a mother garden to prepare cutting materials, comprising the following steps:

[0048] S1 Mother Plant Pruning to Promote Buds: Select a single plant of Moringa oleifera that is more than 3 years old, prune the main stem, wait for the new shoots to grow to 20-30 cm, retain 2-3 axillary buds near the main stem, and then cut the tender shoots at the tip as explants;

[0049] In this step, the main trunk of the mother plant is cut at a height of 1.2 meters from the ground, and is sawed at a 20-degree angle. The saw cut is smooth and wrapped with three layers of plastic wrap to prevent moisture loss.

[0050] In this step, the time for cutting the tender shoots as explants is at noon on a sunny day; 15-20 days after cutting, new shoots can be grown on the reserved bud points of the mother plant and reused.

[0051] S2 explant disinfection pretreatment;

[0052] Cut the explants into 4 cm single bud segments, rinse with tap water for 20 minutes, then wash with hand for 5 minutes with a detergent solution (Libi brand, 10 g stock solution diluted with 700 ml of water), and finally rinse with tap water for 50 minutes. Take out and use a sharp knife to cut off 0.5 cm from the upper and lower incisions of the single bud segment and the position of the bud at the bifurcation of the stem and leaves, leaving a 3 cm long tender stem segment, and disinfect it in the following order: alcohol disinfection for 30 seconds - sterile water washing for 3 minutes, repeat once - 2 drops of Tween + 0.1% mercuric chloride for 9 minutes - sterile water washing for 3 minutes, repeat twice.

[0053] S3 induction culture: Place the sterilized explants flat in sterile bud induction medium at a temperature of 24-26°C, a light intensity of 1500-2000 lx, a light intensity of 9 hours / day, and a humidity of 50-70%. Culture aseptically for 35-40 days to obtain 5-7 cm sterile axillary buds.

[0054] The composition of the induction medium is MS + IBA 0.6 mg / L + NAA 0.2 mg / L + sucrose 15 g / L + agar 5 g / L, pH 6.5;

[0055] S4 rooting culture: Cut the sterile axillary buds into 3 cm long axillary bud segments and insert them into the rooting medium. Control the temperature at 24-26°C, light intensity at 1500-2000 lx, light intensity at 9 h / d, and humidity at 50-70%. Culture aseptically for 35-38 days to obtain rooted seedlings.

[0056] The composition of the rooting medium is MS + IBA 0.2 mg / L + NAA 0.1 mg / L + sucrose 15 g / L + agar 6 g / L + ripe fresh banana 9 g / L + activated carbon 0.6 g / L, pH 5.7;

[0057] S5 seedling hardening and transplanting: Build a moisturizing shed in the greenhouse, transplant the rooted seedlings into the rooting medium in the moisturizing shed, water once in the morning and evening on the 1st to 5th day after transplanting, 50 ml per bag per time, and control the humidity at 95%. On the 6th day, open the moisturizing shed 1 / 3; on the 6th to 10th day, water once in the evening, 50 ml per bag per time; control the humidity at 85%. Open the moisturizing shed fully 15 days after transplanting, and then control the humidity at 70%; during this period, control the temperature of the greenhouse at 25-36℃ and the shade degree at 70%;

[0058] The rooting medium comprises garden soil, river sand and peat soil in a weight ratio of 5:3:2. The rooting medium is mixed evenly and then exposed to the sun for 2-3 days before use.

[0059] In this step of seedling hardening and transplanting, first establish a nursery bed: select a sunny and well-ventilated location in the greenhouse to avoid a dark and humid environment. Use materials such as bricks, river sand, and cement, and select and size them according to needs and actual conditions. The width of the brick bed should not exceed 1.2 meters, and the bed length can be adjusted according to actual conditions. Treat the bottom of the nursery bed and level the bottom of the area selected for the nursery bed. Deeply plow the soil in this area by 20 cm. Deeply plow and loosen the bottom of the nursery bed and then level it. Spread a 10 cm layer of loose coconut husks on it to increase the moisture-retaining and breathable properties of the bottom of the nutrient bag. Build a moisturizing shed on the nursery bed.

[0060] In this step of seedling hardening and transplanting, rooting medium is contained in a nutrient bag, and the nutrient bag is placed on a seedbed covered with coconut husk. In the seedbed, coconut husk is irrigated with water 2 days before use. The nutrient bag is placed and sprayed with 500 times of mancozeb solution on the same day for disinfection. The upper port of the nutrient bag is placed in the seedbed and is 1-2 cm lower than the upper port on the seedbed. This can better water, transplant, and manage the seedlings, and the bag seedlings in the later stage are kept moist, which improves the effect of root growth promotion. After the seedling bags are placed in rows on the seedbed, they are irrigated with a spray pipe for standby use.

[0061] In this step of seedling hardening and transplanting, before transplanting, first move the rooting bottle to the indoor natural light hardening rack and loosen the lid for one day, then open the bottle mouth 1 / 4 and keep it for 2 days before transplanting it into the rooting medium.

[0062] S6 mother garden was established to obtain cutting materials: 45-60 days after the rooted seedlings were transplanted, the Moringa seedlings grew to a height of 25-40 cm, and the stems were cut at a height of 15 cm to obtain the cutting materials;

[0063] The cutting material was further divided into 8 to 10 cm tender stem bud cuttings according to the standard of long internode one bud section, short internode two buds section, and bud section with terminal bud (see attached). Figure 1-3 ).

[0064] The stem-cut plants were planted in a greenhouse with a spacing of 20 × 20 cm to obtain a mother garden.

[0065] Step 2, utilizing the cutting material of step 1 above to propagate the cutting method of Moringa oleifera, comprises the following steps:

[0066] (1) Seven days before cutting the cutting material, spray the plant growth hormone "Amaisu" containing amino acid water-soluble fertilizer once, with a concentration of 20 ml to 15 liters of water, and spray evenly on the front and back of the leaves;

[0067] (2) Cuttings are carried out in a greenhouse with a shade of 70%. The nutrient bag substrate is watered thoroughly on the second day before cutting. The nutrient soil in the bag is turned over with a 25cm long bamboo stick in the afternoon of the day before cutting to a depth of about 7cm. In the evening of the first day after turning over the soil, the materials are cut as required for cuttings. The cutting depth is 3-4cm. Within 30 minutes after cutting, 500 times diluted "Mancozeb" disinfectant solution 100ml / bag is promptly poured. Cover with a transparent moisturizing bottle (specifications: bottle mouth diameter 5.5cm×bottle bottom diameter 9cm×height 13.5cm, volume 750ml, see attached) Figure 4 ) After all the plants are planted, water them to moisten the roots.

[0068] (3) Water and fertilizer management: After cutting, control the temperature at 25-36 degrees. Spray water once in the morning and evening 5 days before cutting, water once in the evening on the 6th to 10th day, and water once every 2-3 days in the later period according to weather conditions. Ensure that the coconut coir in the seedbed and the bottom of the nutrient bag are moist, and control the humidity in the moisturizing bottle to be above 95%; 15-17 days after cutting, remove the moisturizing bottle in the evening (the moisturizing bottle can be recycled continuously), and water it thoroughly after opening the bottle. Control the humidity at 85%-95% on the day, and keep the humidity above 70% in the later period. 60 days after cutting, it can be transplanted to the field for planting.

[0069] In the present invention, when the cutting seedlings grow to 30-50 cm, the stem buds with 1 to 2 axillary buds above 20 cm can be used as a bud segment again as cutting material; 5 to 7 days before the tender stem buds are cut, the petioles and leaves in the middle of the leaf buds in the stem segment are cut off.

[0070] Comparative Example 1

[0071] Test Materials: Select single Moringa trees aged three years or older. Cut the trunk 1.2 meters above the ground and saw it off at a 20-degree angle. Smooth the cut and wrap it with three layers of plastic wrap to prevent moisture loss. Once new shoots emerge from the cut mother plant, select 15-20 cm long shoots for cuttings.

[0072] Cutting method: same as step 2 of Example 1.

[0073] Effect Example 1

[0074] Cutting materials were obtained and cutting management was performed according to the methods of Implementation 1 and Comparative Example 1. Rooting was counted 22 days after cutting. Specifically, the cutting date was April 3, 2024; the bottle opening date was April 20, 2024; and the investigation date was April 25, 2024.

[0075] The results showed that the cutting materials obtained by the micro-cutting asexual propagation technology of the present invention can take root and survive in a variety of cutting media, and have good rooting effect (see Appendix Figure 5 As shown in Tables 1-5 below. However, the cutting materials obtained from Comparative Example 1 without micro-cutting asexual propagation could not take root and form seedlings in various cutting media (see Appendix Figure 6 ), the rooting rate was 0, indicating that the stem segments directly obtained from the mother plant could not take root.

[0076] Table 1 Matrix - coconut bran

[0077]

[0078]

[0079] Table 2 Matrix - Garden Soil

[0080]

[0081]

[0082] Table 3 Matrix-peaty soil

[0083]

[0084]

[0085] Table 4 Matrix - River Sand

[0086]

[0087]

[0088] Table 5 Matrix - garden soil, river sand and peat soil, weight ratio 5:3:2

[0089]

[0090]

[0091] After the tender shoots of the mother plant of the present invention are transplanted into seedlings through tissue culture micro-cutting technology, the stem buds can be repeatedly cut and propagated into seedlings. After the cuttings have formed seedlings, the stem buds can also be repeatedly cut and propagated into seedlings again. By continuously circulating the cutting seedling method, a large cutting system can be established to meet the production needs of large quantities of high-quality seedlings with uniformity.

[0092] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0093] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for preparing Moringa cutting material, characterized in that, The method of using a micro-cutting asexual propagation method of Moringa oleifera shoots to establish a mother garden to prepare cutting materials includes the following steps: S1: Cut the main trunk of the mother plant and wait for the new shoots to grow to 20-30 cm. Cut the tender shoots at the tip as explants. S2 explant disinfection pretreatment; S3 induction culture: Place the sterilized explants flat in sterile bud induction medium and culture them at a temperature of 24-26°C, a light intensity of 1500-2000 lx, a light intensity of 9 hours / day, and a humidity of 50%-70%. Culture them aseptically for 35-40 days to obtain 5-7 cm sterile axillary buds. S4 rooting culture: Cut the sterile axillary buds into 3 cm long axillary bud segments and insert them into the rooting medium for culture. Control the temperature at 24-26°C, the light intensity at 1500-2000 lx, the light intensity at 9 h / d, and the humidity at 50%-70%. Culture the sterile axillary buds for 35-38 days to obtain rooted seedlings. S5 seedling hardening and transplanting: Build a moisturizing shed in the greenhouse and transplant the rooted seedlings into the rooting medium in the moisturizing shed. On the 1st to 5th day after transplanting, water once in the morning and evening and control the humidity to 95%. On the 6th day, open the moisturizing shed 1 / 3; on the 6th to 10th day, water once in the evening and control the humidity to 85%; open the moisturizing shed fully for 15 days after transplanting, and then control the humidity to 70%; during this period, control the temperature of the greenhouse at 25-36°C and the shade degree at 70%; S6 mother garden is established to obtain cutting materials: 45-50 days after the rooted seedlings are transplanted, the Moringa seedlings grow to a height of 25-35 cm, and the stems are cut and planted in a greenhouse to obtain the mother garden; the new tender shoots of the plants in the mother garden are cut to obtain cutting materials.

2. The method for preparing Moringa cutting material according to claim 1, wherein The main trunk of the S1 mother plant was cut at a height of 1.2 meters from the ground and sawed at a 20-degree angle. The sawn edge was smooth and wrapped to prevent water loss. When cutting the tender shoots as explants, 2-3 axillary buds were retained close to the main trunk. The S2 explant disinfection pretreatment method is to cut the explant into 4 cm single bud segments, first rinse with tap water for 20 minutes, then wash with diluted detergent solution by hand for 5 minutes, and finally rinse with tap water for 50 minutes, then cut off 0.5 cm of the upper and lower incisions of the single bud segment and the position of the bud at the bifurcation of the stem and leaves, leaving a 3 cm long tender stem segment, and disinfect in the order of alcohol disinfection for 30 seconds - sterile water washing for 3 minutes, repeat once - 2 drops of Tween + 0.1% mercuric chloride for 9 minutes - sterile water washing for 3 minutes, repeat twice.

3. A method for preparing Moringa cutting material according to claim 1, wherein The composition of the induction medium in S3 is MS + IBA 0.6 mg / L + NAA 0.2 mg / L + sucrose 15 g / L + agar 5 g / L, pH 6.5; The composition of the rooting medium in S4 is MS + IBA 0.2 mg / L + NAA 0.1 mg / L + sucrose 15 g / L + agar 6 g / L + ripe fresh banana 9 g / L + activated carbon 0.6 g / L, pH 5.7; The rooting medium described in S5 is composed of garden soil, river sand and peat soil in a weight ratio of 5:3:

2.

4. The method for preparing a Moringa cutting material according to claim 1, wherein The rooting medium described in S5 is placed in a nutrient bag, and the nutrient bag is placed on a seedbed covered with coconut husks. The coconut husks are watered thoroughly 2 days before the seedbed is used. On the day the nutrient bag is placed, the seedbed is sprayed and disinfected with a 500-fold diluted wettable powder mancozeb solution.

5. A method for preparing Moringa cutting material according to claim 1, wherein When the stem is cut and planted in S6, the stem is cut at a height of 15 cm, and the resulting tender shoots can also be used as cutting materials; Moreover, the cutting materials obtained in S6 were cut into tender stem buds of 8 to 10 cm as cuttings according to the standards of one bud segment for long internodes, two bud segments for short internodes, and segments with terminal buds.

6. A cutting method for propagating Moringa oleifera using the cutting material according to any one of claims 1 to 5, characterized in that: The following steps are involved: (1) Spray "Amaisu" amino acid water-soluble fertilizer once every 7 days before cutting the cutting material, with a concentration of 20 ml to 15 liters of water; (2) Cuttings are carried out in a greenhouse with a shade degree of 70%. The cutting medium is watered thoroughly on the second day before cutting. The cutting medium is stirred 7 cm in the afternoon of the day before cutting. Cuttings are carried out in the evening. The cutting depth is 3-4 cm. Within 30 minutes after cutting, irrigate with 500 times the wettable powder "Mancozeb" solution, cover with a transparent moisturizing bottle, and water to moisten the roots; (3) Water and fertilizer management: After cutting, control the temperature at 25-36 degrees. Spray water once in the morning and evening 5 days before cutting, water once in the evening on the 6th to 10th day, and water once every 2-3 days in the later period according to weather conditions. Ensure that the coconut coir in the seedbed and the bottom of the nutrient bag are moist, and control the humidity in the moisturizing bottle to above 95%; 15-17 days after cutting, remove the moisturizing bottle in the evening, and water it thoroughly after opening the bottle. Control the humidity at 85%-95% on the day, and keep the humidity above 70% in the later period. 60 days after cutting, it can be transplanted to the field for planting.

7. The method of propagating Moringa oleifera by using any of the cutting materials described in claims 1 to 5 according to claim 6, wherein: When the cuttings grow to 30-50cm, you can take the stem buds with 1 to 2 axillary buds above 20cm as a bud segment and use it as cutting material again; 5 to 7 days before taking the tender stem buds for cuttings, cut off the petioles and leaves in the middle of each leaf canopy in the stem segment.

Images