Drainage fluid amylase uninterrupted detection device and detection method thereof

Abstract

The application discloses a drainage liquid amylase uninterrupted detection device and a detection method thereof. The drainage liquid amylase uninterrupted detection device comprises a micro-fluidic chip, the micro-fluidic chip comprises a transparent base, a reaction area and a detection area are arranged on the transparent base, a first hollow steel needle and a second hollow steel needle are connected to the reaction area, the bottom of the first hollow steel needle and the bottom of the second hollow steel needle are respectively connected to a reaction channel through a leading branch, a detection channel is arranged on the detection area, one end of the detection channel is connected to the reaction channel, the connection position of the detection channel and the reaction channel is connected to a third hollow steel needle, the other end of the detection channel is connected to a fourth hollow steel needle, the fourth hollow steel needle is connected to the inlet of a liquid pumping pump, the outlet of the liquid pumping pump is connected to a waste liquid bag, a heating plate is located directly below the reaction area, a light source is located directly below the detection area, and a photosensitive sensor is arranged above or below the light source. The application realizes uninterrupted detection of drainage liquid.

Description

Technical Field

[0001] The present invention relates to the field of medical auxiliary equipment, and in particular to a detection device for amylase. Background Art

[0002] After abdominal surgery, patients typically require an indwelling drainage tube to remove residual or ongoing exudate from the abdominal cavity and alleviate peritoneal infection. The properties and composition of the drainage fluid are important indicators of the condition. The presence of pancreatic fluid in the drainage fluid indicates pancreatic fistula, which is typically determined by measuring amylase in the drainage fluid. Amylase is a hydrolase and the most widely used enzyme in the fermentation industry.

[0003] There are numerous methods for measuring amylase, with the iodine-starch colorimetric method being a common clinical method. Publication No. CN220099019U discloses a device for rapidly detecting pancreatic amylase in drainage fluid. In this patent, a starch solution and iodine solution in a test kit mix to turn blue. A drainage tube is inserted into the catheter, and the drainage fluid flows through the catheter into the test kit and mixes with the blue solution. If the drainage fluid contains pancreatic amylase, the amylase hydrolyzes the starch, and the mixed solution becomes lighter blue or colorless. If the drainage fluid does not contain pancreatic amylase, the mixed solution remains unchanged.

[0004] The disadvantage is that it is impossible to achieve uninterrupted detection of drainage fluid, which is not conducive to improving detection efficiency. Summary of the Invention

[0005] In response to the problems existing in the prior art, the present invention provides a drainage fluid amylase uninterrupted detection device, which has solved at least one of the above technical problems.

[0006] The present invention provides a method for continuous detection of amylase in drainage fluid, which solves at least one of the above technical problems.

[0007] The technical solution of the present invention is: a drainage fluid amylase uninterrupted detection device, characterized in that it includes a microfluidic chip, the microfluidic chip includes a transparent base, the transparent base is provided with a reaction area and a detection area arranged adjacent to each other, the reaction area is provided with a reaction channel for mixing drainage fluid and starch solution, the reaction area is connected to a first hollow steel needle arranged vertically and used for introducing drainage fluid and a second hollow steel needle arranged vertically and used for introducing starch solution, the bottom of the first hollow steel needle and the bottom of the second hollow steel needle are respectively connected to the reaction channel through an introduction branch;

[0008] The top of the first hollow steel needle is connected to a drainage tube for conducting drainage fluid through a flexible tube;

[0009] The top of the second hollow steel needle is connected to a starch solution bag storing starch solution through a hose;

[0010] The detection area is provided with a detection channel, one end of the detection channel is connected to the reaction channel, the docking point of the detection channel and the reaction channel is connected to a vertically arranged third hollow steel needle, the third hollow steel needle is used to introduce iodine solution, the other end of the detection channel is connected to a vertically arranged fourth hollow steel needle, the fourth hollow steel needle is connected to the inlet of the liquid pump, and the outlet of the liquid pump is connected to a waste liquid bag;

[0011] The top of the third hollow steel needle is connected to an iodine liquid bag storing iodine liquid through a hose;

[0012] The microfluidic chip is further provided with an intermediate support, wherein a support plate is provided on the intermediate support, the support plate is detachably connected to the microfluidic chip, and an opening is provided in the center of the support plate for exposing the reaction area and the detection area;

[0013] The device further comprises a shell, the shell being detachably connected to the intermediate bracket and enclosing an upper cavity and a lower cavity separated by the support plate; a heating plate and a light source being detachably connected to the shell, the heating plate and the light source being located in the lower cavity, the heating plate being located directly below the reaction zone, and the light source being located directly below the detection zone; and a liquid pump being detachably connected to the shell, the liquid pump being located on the periphery of the lower cavity.

[0014] It also includes a flip cover, which is hinged to the shell and used to block the top of the upper cavity. A photosensor is installed on the flip cover, and the photosensor and the light source are arranged up and down.

[0015] The present invention realizes uninterrupted detection of drainage fluid. The present invention facilitates uninterrupted extraction of liquid through a liquid pump, and realizes that drainage fluid, starch and iodine solution are driven by the liquid pump to pass through the reaction area and the detection area and then enter the waste liquid bag for collection.

[0016] Further preferably, the bag openings of the starch solution bag, the iodine solution bag and the waste liquid bag are all installed with one-way valves.

[0017] Further preferably, an interface is provided on the shell, the interface is higher than the top of the first hollow steel needle, the drainage tube is connected to the interface, and the interface is connected to the first hollow steel needle through a hose.

[0018] It is convenient to switch different drainage tubes.

[0019] Further preferably, the interface switch is connected to the drainage tube and the flushing tube.

[0020] It is convenient to clean the inside of the microfluidic chip when replacing different drainage tubes to avoid interference.

[0021] Further preferably, the intermediate bracket includes a horizontally arranged top plate, adjacent corners of the top plate are respectively provided with downwardly extending support baffles and detection ports, and the other two corners of the top plate are overlapped on the shell;

[0022] The outer edge of the detection port is connected to the support plate through a connecting portion.

[0023] This facilitates the assembly of the intermediate bracket and the shell.

[0024] Further preferably, the connecting portion includes a first enclosure and a second enclosure fixed on adjacent sides of the support plate, and the first enclosure, the second enclosure and adjacent side walls of the housing form an enclosure structure surrounding the periphery of the microfluidic chip;

[0025] The first enclosure or the second enclosure is provided with a placement slot for placing starch solution bags, iodine solution bags and waste liquid bags.

[0026] Further preferably, a front placement slot for placing a starch solution bag and an iodine solution bag is provided on a side of the first enclosure adjacent to the microfluidic chip, and a back placement slot for placing a waste liquid bag is provided on a side of the first enclosure away from the microfluidic chip;

[0027] The front placement slot and the back placement slot are arranged with their openings facing each other;

[0028] The notch of the back placement groove faces the liquid pump.

[0029] Further preferably, the shell is provided with a concave boss, the top plate is overlapped on the boss, a notch is provided on the top plate, a positioning protrusion is provided on the boss and is embedded in the notch, and the top plate and the boss are abutted against each other by a locking screw passing through the shell.

[0030] Further preferably, a lower groove with an opening facing downward is formed at the bottom of the housing, the lower groove is detachably connected to a bottom cover plate for sealing the notch of the lower groove, and the lower groove and the bottom cover plate are detachably connected to form an accommodating cavity for accommodating the controller;

[0031] The controller is connected to the light source, the photosensor, the heating plate, the display screen and the DC motor driver through wires, and the DC motor driver controls the DC motor connected to the liquid pump;

[0032] The display screen is installed on the flip cover.

[0033] Further preferably, the side wall of the housing is provided with a side groove opening outward, the side groove is located at the boss, and the notch of the side groove is slidably connected to a sliding cover for sealing the notch of the side groove;

[0034] The side groove and the sliding cover are detachably connected to form a receiving cavity for receiving a battery;

[0035] The battery is connected to the power input terminal of the controller.

[0036] The method for continuous detection of amylase in drainage fluid is characterized in that the detection is performed using the device for continuous detection of amylase in drainage fluid;

[0037] After the heating plate is started and heated to the set temperature, the liquid pump is started, which drives the drainage liquid and starch solution in the drainage tube to converge and mix into the reaction channel, and the iodine solution enters the detection channel;

[0038] After the liquid pump stops for a set time, it continues to work and transports the liquid in the reaction channel to the detection channel, and the iodine solution also enters the detection channel simultaneously;

[0039] The sensing information of the photosensor is compared with the information stored in the database. The database stores the sensing information of the photosensor corresponding to the amylase content in different drainage fluids. The controller analyzes and obtains the amylase content information in the database that best matches the sensing information of the current photosensor, and takes the amylase content information that best matches as the amylase content information in the current drainage fluid.

[0040] Further preferably, when the sensing information of the photosensor exceeds a set time and the variation deviation of the sensing information is lower than a set value, the controller compares the sensing information of the photosensor with the information stored in the database.

[0041] Further preferably, the light source is an LED light source with variable color temperature and chromaticity;

[0042] The database stores calibration sensing information of the photosensor at different color temperatures and different chromaticities under the same amylase content;

[0043] When the controller obtains the amylase content information, the controller controls the liquid pump to pause and switches the LED light source;

[0044] If the sensing signal obtained by the photosensor matches the photosensor calibration sensing information under the current amylase content information in the database, it means that the detection is successful;

[0045] If there is a mismatch, the controller reports an error.

[0046] Beneficial effects:

[0047] The present invention realizes detection during the uninterrupted delivery of drainage fluid. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 This is a structural diagram of a specific embodiment 1 of the present invention;

[0049] Figure 2 This is a schematic diagram of the partial structure of a specific embodiment 1 of the present invention;

[0050] Figure 3 This is an exploded view of a specific embodiment 1 of the present invention;

[0051] Figure 4 A cross-sectional view of a specific embodiment 1 of the present invention;

[0052] Figure 5 This is a structural schematic diagram of a housing according to a specific embodiment 1 of the present invention;

[0053] Figure 6 This is a structural schematic diagram of the intermediate bracket of the specific embodiment 1 of the present invention;

[0054] Figure 7 This is a schematic diagram of the partial structure of specific embodiment 1 of the present invention.

[0055] In the figure: 1 is the shell, 2 is the middle bracket, 3 is the flip cover, 4 is the microfluidic chip, 5 is the liquid pump, 6 is the heating plate, 7 is the light source, 8 is the sliding cover, 9 is the photosensor, 10 is the button, 11 is the interface, 12 is the boss, 13 is the controller, 14 is the bottom cover, 21 is the front placement groove, 22 is the back placement groove, 23 is the top plate, 24 is the notch, 41 is the first hollow steel needle, 42 is the second hollow steel needle, 43 is the third hollow steel needle, 44 is the fourth hollow steel needle, 45 is the reaction channel, and 46 is the detection channel. DETAILED DESCRIPTION

[0056] See also Figures 1 to 7Specific embodiment 1, drainage fluid amylase uninterrupted detection device, including a microfluidic chip 4, the microfluidic chip 4 includes a transparent base, the transparent base is provided with a reaction zone and a detection zone arranged adjacent to each other, the reaction zone is provided with a reaction channel 45 for mixing drainage fluid and starch solution, the reaction zone is connected to a first hollow steel needle 41 vertically arranged and used for introducing drainage fluid and a second hollow steel needle 42 vertically arranged and used for introducing starch solution, the bottom of the first hollow steel needle 41 and the bottom of the second hollow steel needle 42 are respectively connected to each other. The top of the first hollow steel needle 41 is connected to the drainage tube for conducting drainage fluid through a hose; the top of the second hollow steel needle 42 is connected to the starch solution bag storing starch solution through a hose; a detection channel 46 is provided on the detection area, one end of the detection channel 46 is connected to the reaction channel 45, and the docking point of the detection channel 46 and the reaction channel 45 is connected to the vertically arranged third hollow steel needle 43, the third hollow steel needle 43 is used to introduce iodine solution, and the other end of the detection channel 46 is connected The fourth hollow steel needle 44 is connected to the inlet of the liquid pump 5, and the outlet of the liquid pump 5 is connected to a waste liquid bag; the top of the third hollow steel needle 43 is connected to the iodine liquid bag storing iodine liquid through a hose; the middle bracket 2 is provided with a support plate, the support plate is detachably connected to the microfluidic chip 4, and the center of the support plate is provided with an opening for exposing the reaction area and the detection area; the shell 1 is also included, the shell 1 is detachably connected to the middle bracket 2, and forms a through hole. The housing 1 comprises an upper cavity and a lower cavity separated by a support plate. Adjacent heating plates and a light source 7 are detachably connected to the housing 1. The heating plates and light source 7 are located in the lower cavity, with the heating plates located directly below the reaction zone and the light source 7 located directly below the detection zone. A liquid extraction pump 5 is detachably connected to the housing 1 and positioned peripherally around the lower cavity. The housing 1 also includes a flip cover 3 hingedly connected to the housing 1 and used to seal the top of the upper cavity. A photosensor 9 is mounted on the flip cover 3, and the photosensor 9 and light source 7 are positioned above each other. The present invention facilitates uninterrupted liquid extraction through the liquid extraction pump 5, allowing drainage fluid, starch, and iodine solution to flow through the reaction zone and detection zone and into a waste liquid bag for collection. An opening is provided in the center of the support plate to expose the reaction zone and detection zone. This opening exposes both the upper and lower sides of the reaction zone and detection zone. Light is transmitted to the photosensor via the opening below the detection zone, the microfluidic chip, and the opening above the detection zone.

[0057] One-way valves are installed at the bag openings of starch solution bags, iodine solution bags and waste liquid bags.

[0058] The housing 1 is provided with an interface 11, which is higher than the top of the first hollow steel needle 41. The drainage tube is docked with the interface 11, and the interface is connected to the first hollow steel needle 41 through a hose, so as to facilitate switching between different drainage tubes.

[0059] The interface switches and connects the drainage tube and the flushing tube, making it easy to clean the inside of the microfluidic chip 4 when replacing different drainage tubes, avoiding interference.

[0060] The intermediate bracket 2 includes a horizontally arranged top plate. Adjacent corners of the top plate are provided with downwardly extending support baffles and a detection port. The other two corners of the top plate overlap the housing 1. The outer edge of the detection port is connected to the support plate via a connector, facilitating assembly of the intermediate bracket 2 and the housing 1.

[0061] The connecting part includes a first enclosure and a second enclosure fixed on adjacent sides of the support plate. The first enclosure, the second enclosure and the adjacent two side walls of the shell 1 form an enclosure structure arranged around the periphery of the microfluidic chip 4; the first enclosure or the second enclosure is equipped with a placement slot for placing starch solution bags, iodine solution bags and waste liquid bags.

[0062] A front placement slot 21 for placing starch solution bags and iodine solution bags is provided on the side of the first enclosure adjacent to the microfluidic chip 4, and a back placement slot 22 for placing waste liquid bags is provided on the side of the first enclosure away from the microfluidic chip 4; the notches of the front placement slot 21 and the back placement slot 22 are arranged opposite to each other; the notch of the back placement slot 22 is directly opposite to the liquid pump 5.

[0063] The housing 1 is provided with a concave boss 12, and the top plate is overlapped on the boss 12. A notch 24 is provided on the top plate 23, and a positioning protrusion is provided on the boss to fit into the notch. The top plate 23 and the boss 12 are abutted against each other by locking screws passing through the housing 1. The detection port exposes the notch of the front placement slot. An opening is provided on the top plate for exposing the notch of the back placement slot. This facilitates visual observation of the contents of each starch solution bag, iodine solution bag, and waste liquid bag. The notch of the back placement slot faces forward. The notch of the front placement slot faces backward. The rear of the housing is hinged to the flip cover. The rear side panel of the housing is provided with an interface.

[0064] A lower groove with an opening facing downward is provided at the bottom of the shell 1, and the lower groove is detachably connected to a bottom cover plate 14 for sealing the notch of the lower groove. The lower groove and the bottom cover plate 14 are detachably connected to a receiving cavity for accommodating a controller 13; the controller 13 is connected to the light source 7, the photosensor 9, the heating plate 6, the display screen and the DC motor driver through wires, and the DC motor driver controls the DC motor connected to the liquid pump 5; a display screen is installed on the flip cover 3.

[0065] The sidewall of the housing 1 is formed with an outwardly opening side groove located at the boss. A sliding cover 8 is slidably connected to the side groove to seal the notch. The side groove and the sliding cover 8 are detachably connected to form a chamber for accommodating a battery. The battery is connected to the power input terminal of the controller. The light source is an LED.

[0066] A light source and a heating plate are installed at the left rear of the housing. A liquid pump is installed at the left front of the housing, a battery is installed on the right side of the housing, and a controller is installed at the bottom of the housing.

[0067] The housing 1 is rotatably connected to a button 10 via a shaft. A torsion spring is mounted on the outer periphery of the shaft. The button and the flip cover are provided with matching buckles and slots for easy interlocking.

[0068] The present invention relates to a method for continuously detecting amylase in drainage fluid, using a continuous detection device for detecting amylase in drainage fluid. After a heating plate is started and heated to a set temperature, a pump is started. The pump drives the drainage fluid and starch solution in the drainage tube to converge and mix into a reaction channel, and iodine solution enters the detection channel. After the pump stops for a set time, the pump resumes operation, transporting the liquid in the reaction channel to the detection channel, and iodine solution also enters the detection channel simultaneously. The sensing information of the photosensor is compared with information stored in a database. The database stores sensing information of the photosensor corresponding to different amylase levels in the drainage fluid. A controller analyzes and obtains the amylase content information in the database that best matches the sensing information of the current photosensor, and the best matching amylase content information is used as the amylase content information in the current drainage fluid. The set temperature is preferably 60°.

[0069] When the sensing information of the photosensor exceeds the set time and the change deviation of the sensing information is lower than the set value, the controller compares the sensing information of the photosensor with the information stored in the database.

[0070] The light source is an LED light source with variable color temperature and chromaticity; the database stores the calibrated sensing information of the photosensor at different color temperatures and different chromaticities under the same amylase content; when the controller obtains the amylase content information, the controller controls the liquid pump to pause and switch the LED light source; if the sensing signal obtained by the photosensor matches the calibrated sensing information of the photosensor under the current amylase content information in the database, it means that the detection is successful; if it does not match, the controller will report an error.

[0071] Beneficial effects:

[0072] The present invention realizes detection during the uninterrupted delivery of drainage fluid.

[0073] The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Drainage fluid amylase uninterrupted detection device, characterized in that: The invention comprises a microfluidic chip, wherein the microfluidic chip comprises a transparent base, wherein a reaction zone and a detection zone are adjacently arranged on the transparent base, wherein a reaction channel for mixing drainage fluid and starch solution is provided on the reaction zone, wherein a first hollow steel needle arranged vertically and used for introducing drainage fluid and a second hollow steel needle arranged vertically and used for introducing starch solution are connected to the reaction zone, wherein the bottom of the first hollow steel needle and the bottom of the second hollow steel needle are respectively connected to the reaction channel via an introduction branch; The top of the first hollow steel needle is connected to a drainage tube for conducting drainage fluid through a flexible tube; The top of the second hollow steel needle is connected to a starch solution bag storing starch solution through a hose; The detection area is provided with a detection channel, one end of the detection channel is connected to the reaction channel, the docking point of the detection channel and the reaction channel is connected to a vertically arranged third hollow steel needle, the third hollow steel needle is used to introduce iodine solution, the other end of the detection channel is connected to a vertically arranged fourth hollow steel needle, the fourth hollow steel needle is connected to the inlet of the liquid pump, and the outlet of the liquid pump is connected to a waste liquid bag; The top of the third hollow steel needle is connected to an iodine liquid bag storing iodine liquid through a hose; The microfluidic chip is further provided with an intermediate support, wherein a support plate is provided on the intermediate support, the support plate is detachably connected to the microfluidic chip, and an opening is provided in the center of the support plate for exposing the reaction area and the detection area; The device further comprises a shell, the shell being detachably connected to the intermediate bracket and enclosing an upper cavity and a lower cavity separated by the support plate; a heating plate and a light source being detachably connected to the shell, the heating plate and the light source being located in the lower cavity, the heating plate being located directly below the reaction zone, and the light source being located directly below the detection zone; and a liquid pump being detachably connected to the shell, the liquid pump being located on the periphery of the lower cavity. It also includes a flip cover, which is hinged to the shell and used to block the top of the upper cavity. A photosensor is installed on the flip cover, and the photosensor and the light source are arranged up and down.

2. The drainage fluid amylase uninterrupted detection device according to claim 1, characterized in that: The shell is provided with an interface, which is higher than the top of the first hollow steel needle. The drainage tube is connected to the interface, and the interface is connected to the first hollow steel needle through a hose.

3. The drainage fluid amylase uninterrupted detection device according to claim 2, characterized in that: The interface switch connects the drainage tube and the flushing tube.

4. The drainage fluid amylase uninterrupted detection device according to claim 1, characterized in that: The intermediate bracket includes a horizontally arranged top plate, adjacent corners of the top plate are respectively provided with downwardly extending support baffles and detection ports, and the other two corners of the top plate are overlapped on the shell; The outer edge of the detection port is connected to the support plate through a connecting portion.

5. The drainage fluid amylase uninterrupted detection device according to claim 4, characterized in that: The connecting portion includes a first enclosure and a second enclosure fixed on two adjacent sides of the support plate, and the first enclosure, the second enclosure and the adjacent two side walls of the housing form an enclosure structure surrounding the periphery of the microfluidic chip; The first enclosure or the second enclosure is provided with a placement slot for placing starch solution bags, iodine solution bags and waste liquid bags.

6. The drainage fluid amylase uninterrupted detection device according to claim 5, characterized in that: A front placement slot for placing a starch solution bag and an iodine solution bag is provided on a side of the first enclosure adjacent to the microfluidic chip, and a back placement slot for placing a waste liquid bag is provided on a side of the first enclosure away from the microfluidic chip; The front placement slot and the back placement slot are arranged with their openings facing each other; The notch of the back placement groove faces the liquid pump.

7. The drainage fluid amylase uninterrupted detection device according to claim 4, characterized in that: The shell is provided with a concave boss, the top plate is overlapped on the boss, a notch is provided on the top plate, a positioning protrusion is provided on the boss and is embedded in the notch, and the top plate and the boss are abutted against each other by a locking screw passing through the shell.

8. The drainage fluid amylase uninterrupted detection device according to claim 7, characterized in that: The bottom of the housing is provided with a lower groove with an opening facing downward, the lower groove is detachably connected to a bottom cover plate for blocking the notch of the lower groove, and the lower groove and the bottom cover plate are detachably connected to form an accommodating cavity for accommodating the controller; The controller is connected to the light source, the photosensor, the heating plate, the display screen and the DC motor driver through wires, and the DC motor driver controls the DC motor connected to the liquid pump; The display screen is installed on the flip cover; The side wall of the housing is provided with a side groove with an outward opening, the side groove is located at the boss, and a sliding cover is slidably connected to the notch of the side groove for sealing the notch of the side groove; The side groove and the sliding cover are detachably connected to form a receiving cavity for receiving a battery; The battery is connected to the power input terminal of the controller.

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