A bactericide for preventing and treating bacterial wilt of solanaceous vegetables containing oil tea shell

Abstract

The present application belongs to the technical field of prevention and treatment of Ralstonia solanacearum of Solanaceae vegetables, and particularly relates to a fungicide containing oil tea shells for preventing and treating Ralstonia solanacearum of Solanaceae vegetables. A fungicide composition is formed by dual-compounding of berberine and aureobasidin or triforine. The present application performs dual-compounding of berberine and aureobasidin or triforine, determines the activity of the dual-compound formula on Ralstonia solanacearum by using the filter paper disc diffusion method in a room, and evaluates the action type of the dual-compound formula by using the Sun Yunpei method. It is found that, in a certain mass ratio range, the co-toxicity coefficient of the dual-compound formula on Ralstonia solanacearum is greater than 120, and the dual-compound formula shows a synergistic effect. Based on this, the prevention and treatment effect on Ralstonia solanacearum of Solanaceae vegetables can be improved, the use dose of the pesticide can be reduced, the generation and accumulation of the drug resistance of the pathogenic bacteria can be delayed, and the development of a new type of high-efficiency and safe pesticide can be facilitated.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of prevention and treatment of Ralstonia solancearum species complex (RSSC) in Solanaceae vegetables, and particularly relates to a bactericide containing oil-tea camellia shell for preventing and treating Ralstonia solancearum species complex (RSSC) in Solanaceae vegetables. BACKGROUND

[0002] Ralstonia solancearum species complex (RSSC) is a kind of soil-borne bacterial disease, which can harm more than 450 crops of 54 families, especially Solanaceae vegetables, including peppers, tomatoes and eggplants. The pathogenic mechanism and pathogenic conditions of Ralstonia solancearum species complex (RSSC) are complex and diverse, and it is difficult to prevent and control.

[0003] At present, chemical control is still the main means to prevent and control Ralstonia solancearum species complex (RSSC) in Solanaceae vegetables. The conventional chemical agents on the market for preventing and treating Ralstonia solancearum species complex (RSSC) in Solanaceae vegetables mainly include organic copper, inorganic copper, organochlorine, inorganic sulfur and organosulfur bactericides. Although chemical agents have the advantages of convenient application and quick effect, long-term application of single-component agents can easily lead to pathogen resistance, resulting in reduced control effect of the agents. Although increasing the dosage of the agents per unit area can improve the control effect, such control effect is only short-term, and such application method can easily aggravate the accumulation and development of pathogen resistance, leading to further reduction of the control effect of the agents, even failure, and also causing environmental pollution and pesticide residues. Therefore, it is of great significance to develop high-efficiency and safe agents.

[0004] Berberine, also known as berberine, is a common isoquinoline alkaloid, which exists in many plants of four families and ten genera of Berberidaceae and other families. It was first obtained from Xanthoxylon clava bark by M.-E. Chavayere and G. Pertan in 1826. Camellia oleifera is a unique woody oil plant in southern China, and is known as one of the world's four major woody oil plants together with olive, oil palm and coconut. Camellia shell is the pericarp of Camellia fruit and is the main by-product of Camellia processing, accounting for 50-60% of the weight of the entire Camellia fruit. The Camellia shell produced every year is as high as 3 million tons, but most of it is usually discarded or burned as fuel and has not been fully utilized. Camellia shell is rich in various active ingredients, containing 4-8% tea saponin, which is a natural non-ionic surfactant with foaming, emulsifying, anti-inflammatory and antibacterial effects, and is also a good pesticide adjuvant, widely used in the fields of pesticides, cleaning and care products, etc. Compounding different pesticides is an effective and convenient way to develop new agents. There are related reports that berberine can be used to prevent and control bacterial diseases, but there is no related report on the compounding of berberine with aureofungin or flutriafol.

[0005] The information disclosed in this Background section is only for the purpose of increasing an understanding of the general context of the present application and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art that is already known in any country in the world. SUMMARY

[0006] The present application aims to provide an oil-tea camellia shell-containing fungicide for preventing and treating bacterial wilt of solanaceous vegetables to solve the problems existing in long-term application of single-component fungicides.

[0007] To achieve the above-mentioned purpose, the present application provides the following technical solutions.

[0008] The first object of the present application is to provide a fungicidal composition formed by binary compounding of berberine and aureobasidin or hexaconazole.

[0009] Preferably, the mass ratio of berberine to aureobasidin is 1-5:7-1.

[0010] Preferably, the mass ratio of berberine to hexaconazole is 1-12:12-1.

[0011] The second object of the present application is to provide an oil-tea camellia shell-containing fungicide for preventing and treating bacterial wilt of solanaceous vegetables, which is prepared by taking the fungicidal composition as an active ingredient and adding oil-tea camellia shell and an auxiliary ingredient allowed in pesticide science.

[0012] Preferably, the pesticide preparation is prepared by taking the fungicidal composition 0.5-30%, oil-tea camellia shell 10-15%, dispersing agent 3-8%, wetting agent 1-6% and filler supplementing to 100% by mass percentage.

[0013] Preferably, the dispersing agent is sodium lignosulfonate; the wetting agent is calcium dodecylbenzenesulfonate; and the filler is formed by mixing attapulgite and sepiolite at a mass ratio of 1:3.

[0014] The third object of the present application is to provide the use of the fungicidal composition or the oil-tea camellia shell-containing fungicide for preventing and treating bacterial wilt of solanaceous vegetables in preventing and treating bacterial wilt of solanaceous vegetables.

[0015] Compared with the prior art, the present application has the following beneficial effects:

[0016] The berberine is compounded with the aureobasidin or the dicarboximide, the activity of the compound formula on the solanaceae ralstonia solancearum species complex is determined by using the filter paper disc diffusion method in the room, and the action type of the compound formula is evaluated by using the Sun Yunpei method. The results show that in a certain mass ratio range, the synergistic coefficient of the compound formula on the solanaceae ralstonia solancearum species complex is greater than 120, and the synergistic effect is shown. Based on this, the prevention and treatment effect on the solanaceous vegetable bacterial wilt can be improved, the use dose of the pesticide is reduced, the accumulation of the drug resistance of the pathogenic bacteria is delayed, and the development of the new type of efficient and safe pesticide is helpful. DETAILED DESCRIPTION

[0017] The technical solutions of the patent of the application will be described below clearly and completely. Obviously, the described embodiments are part of the embodiments of the application, rather than all the embodiments. Based on the embodiments in the application, all other embodiments obtained by the person skilled in the art without creative labor belong to the protection scope of the application.

[0018] Example : Indoor activity test

[0019] 1. Test pathogen

[0020] Solanaceae ralstonia solancearum species complex (RSSC)

[0021] 2. Culture medium

[0022] NA culture medium: 10 g of proteose peptone, 3 g of beef extract, 5 g of sodium chloride, 20 g of agar, and distilled water is added to 1 L, pH 7.0.

[0023] NB culture medium: 10 g of proteose peptone, 3 g of beef extract, 5 g of sodium chloride, and distilled water is added to 1 L, pH 7.0.

[0024] 3. Test agent

[0025] 98% berberine technical, 94% aureobasidin technical, 96.5% dicarboximide technical, all the above agents are commercially available.

[0026] After the test agent is dissolved, the mass fraction 0.1% of the Tween-80 aqueous solution is diluted into 1000 mg / L of a single agent stock solution, and then a plurality of groups of ratios are set, and 5 series of mass concentration gradients of each single agent and each group of mixed agents are set.

[0027] 4. Test method

[0028] Filter paper diffusion method was adopted. Ralstonia solancearum species complex (RSSC) was activated and cultured on NA medium, and then was expanded. Then, the RSSC suspension with a concentration of 1.0 x 10 8 cfu / mL was prepared by using sterile water. When the temperature of the NA medium to be sterilized was reduced to about 50 DEG C, 10 mL was poured into a culture dish (9 cm in diameter), shaken, cooled, and then flat-plate was prepared. 50 uL of the RSSC suspension was taken and uniformly coated on the NA flat-plate. A puncher was used to punch a filter paper piece with a diameter of 6 mm, the filter paper piece was sterilized, soaked in the drug solution for 1 h, taken out, and the excess drug solution was drained, and then placed in the center of the bacterial flat-plate, 1 filter paper piece was placed on each NA flat-plate, and the filter paper piece soaked in sterile water was used as a blank control, and each treatment was repeated 4 times. Then, the culture was continued in a bacterial incubator at 28 DEG C. After 48 h, the diameter of the inhibition zone was measured, and the inhibition rate was calculated.

[0029]

[0030] 4. Data analysis

[0031] The concentration of the drug was converted into a logarithmic value, the corresponding inhibition rate was converted into a probability value, a regression equation of virulence, EC 50 value and correlation coefficient were calculated with the logarithmic value of the concentration as the independent variable and the probability value of the inhibition rate as the dependent variable. The synergistic coefficient (CTC value) of the mixed agent was calculated according to the method of Sun Yunpei, and the synergistic effect of the drug was evaluated according to the calculated synergistic coefficient (CTC value): CTC≤80 is antagonistic effect, 80<CTC<120 is additive effect, and CTC≥120 is synergistic effect. The results are shown in Tables 1-2.

[0032] Table 1 Indoor bioactivity determination results of berberine and gennimycin complex on Ralstonia solancearum species complex

[0033] Medicament name and ratio EC 50 (mg / L) ATI TTI CTC Berberine 2.3271 100.0000 -- -- Goldnucleomycin 9.3614 24.8585 -- -- Berberine 1 : Goldnucleomycin 7 5.2346 44.4561 34.2512 129.7945 Berberine 1 : Goldnucleomycin 5 3.4445 67.5599 37.3821 180.7281 Berberine 1 : Goldnucleomycin 3 3.9739 58.5596 43.6438 134.1761 Berberine 1 : Goldnucleomycin 1 3.0535 76.2109 62.4292 122.0757 Berberine 3 : Goldnucleomycin 1 2.0612 112.9003 81.2146 139.0147 Berberine 5 : Goldnucleomycin 1 1.5984 145.5893 87.4764 166.4327

[0034] As shown in Table 1, after the berberine and gennimycin were complexed, the synergistic coefficients of the complex on Ralstonia solancearum species complex were all greater than 120 in the mass ratio range of 1-5:7-1, which showed synergistic effect. It is indicated that the bactericidal composition of the complex of the berberine and gennimycin can improve the prevention and treatment effect on Ralstonia solancearum species complex.

[0035] Table 2 Indoor bioactivity determination results of berberine and tridemorph complex on Ralstonia solancearum species complex

[0036] Medicament name and ratio EC 50 (mg / L) ATI TTI CTC Berberine 2.3271 100.0000 -- -- Cetragi 24.0469 9.6773 -- -- Berberine 1 : Cetragi 12 10.8414 21.4649 16.6252 129.1106 Berberine 1 : Cetragi 8 7.7786 29.9167 19.7132 151.7598 Berberine 1 : Cetragi 4 5.8747 39.6122 27.7419 142.7886 Berberine 1 : Cetragi 1 3.4819 66.8342 54.8387 121.8742 Berberine 4 : Cetragi 1 1.4927 155.8987 81.9355 190.2701 Berberine 8 : Cetragi 1 2.0276 114.7712 89.9641 127.5743 Berberine 12 : Cetragi 1 1.9914 116.8575 93.0521 125.5829

[0037] As shown in Table 2, the co-toxicity coefficients of berberine and thiabendazole in the mass ratio range of 1-12:12-1 to Ralstonia solanacearum are all greater than 120, which shows synergistic effect. It is indicated that the fungicidal composition prepared by compounding berberine and thiabendazole can improve the control effect on the bacterial wilt of Solanaceae.

[0038] In summary, the present application compounds berberine with aureofungin or thiabendazole, determines the activity of the compound formula to Ralstonia solanacearum by using filter paper disc diffusion method in the laboratory, and evaluates the action type of the compound formula by Sun Yunpei method. It is found that the co-toxicity coefficient of the compound formula to Ralstonia solanacearum is greater than 120 in a certain mass ratio range, which shows synergistic effect. Based on this, the control effect on the bacterial wilt of Solanaceae vegetables can be improved, the use dose of pesticide can be reduced, the accumulation of pathogenic bacteria resistance can be delayed, which is helpful for developing new high-efficiency and safe pesticides.

[0039] The foregoing description of specific exemplary embodiments of the application is intended to be illustrative only and is not intended to limit the application to the precise forms described. Many modifications and variations are possible in light of the above teachings without departing from the spirit or essential characteristics of the present application. The exemplary embodiments were chosen and described in order to explain the principles of the application and its practical application and to allow others skilled in the art to understand the application for various exemplary embodiments with various modifications being applicable. The scope of the application is intended to be defined by the claims and their equivalents.

Claims

1. A bactericidal composition, characterized in that, The bactericidal composition is a binary compound of berberine and aureomycin; the mass ratio of berberine to aureomycin is 1-5:7-1.

2. A fungicide containing camellia oleifera shell for controlling bacterial wilt of solanaceous vegetables, characterized in that, It is prepared using the bactericidal composition of claim 1 as the active ingredient, supplemented with camellia oleifera shell and auxiliary ingredients permitted to be added in pesticide science; It is prepared from the following components by weight percentage: 0.5-30% of the bactericidal composition, 10-15% of camellia oleifera shell, 3-8% of dispersant, 1-6% of wetting agent, and filler to 100%; The dispersant is sodium lignosulfonate; the wetting agent is calcium dodecylbenzenesulfonate; and the filler is a mixture of attapulgite and sepiolite in a mass ratio of 1:

3.

3. The use of the bactericidal composition of claim 1 or the fungicide containing camellia oleifera shell for controlling bacterial wilt of solanaceous vegetables of claim 2 in the control of bacterial wilt of solanaceous vegetables, characterized in that, The pathogen causing bacterial wilt in solanaceous vegetables is *Ralstonia solanaceae* (Solanaceae). Ralstonia solanacearum (species complex).

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