Dendrobium candidum substitute tea and preparation method thereof

Abstract

The application relates to the technical field of processing of substitute tea, in particular to a gancao and dendrobium substitute tea and a preparation method thereof, which is composed of the following components in parts by weight: gancao 30-40 parts, dendrobium 25-33 parts, papaya powder 20-23 parts, rehmannia 10-20 parts, angelica 10-20 parts, white peony root 10-20 parts, isatis root 4-9 parts, forsythia 3-7 parts and mulberry leaves 5-10 parts. The gancao and dendrobium substitute tea prepared by the application is rich in nutrition, has high amino acid content, contains various effective components such as minerals, flavones and polysaccharides, has good taste, can promote absorption of the body, improve cell activity, improve liver function and has good protection on acute liver injury.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of processing of substitute tea, in particular to a kind of Lithocarpus Polystachys Rehd Tea and its preparation method. BACKGROUND

[0002] Lithocarpus Polystachys Rehd Tea is a kind of pure natural beverage that Miao, Dong, Tujia and Gelao ethnic groups in Guizhou have long loved, with golden soup color, sweet and mellow fragrance, and sweet and delicious taste, which is used as the best beverage to treat distinguished guests. It is processed from the tender leaves of a kind of wild plant with sweet taste, so it is called sweet tea or Lithocarpus Polystachys Rehd Tea in common, the scientific name is Lithocarpus Polystachys Rehd Tea, the Latin name is Lithocarpus Polystachys Rehd Tea, and it belongs to Fagaceae and Lithocarpus, and is evergreen. It is widely distributed on Guizhou plateau, and the resource quantity is extremely large. Lithocarpus Polystachys Rehd Tea is planted on a large scale in Guizhou, and the yield and quality are excellent, and it has a good reputation in the market.

[0003] At present, how to use Lithocarpus Polystachys Rehd Tea and Dendrobium effectively to develop a beverage with better market prospect is one of the research hotspots. For example, a kind of preparation method of weight loss milk tea is disclosed in patent No. CN201810455078.0, in which, areca, astragalus and Dendrobium are crushed, heated with strong fire, taken out when they are crisp, dried, crushed into fine powder, sieved, and then medicinal powder is obtained. Lithocarpus Polystachys Rehd Tea, melastoma dodecandrum and mulberry bark are crushed respectively, mixed, boiled with water, cooled, then cellulase is added, extracted, enzyme is inactivated, filtered, and medicinal liquid is obtained. The medicinal powder is mixed uniformly with oolong tea powder, milk powder, celery powder, tremella powder, corn powder and red bean powder, then medicinal liquid and hot water are added, stirred, steamed to remove water, and the solid components are dried and ground, sieved, and then weight loss milk tea is obtained, which has the effect of detoxification and weight loss. As another example, a kind of health tea for benefiting intestines and stomach and its preparation method is disclosed in patent No. CN201810508886.9, which is made of 10-16 parts of osmanthus, 8-13 parts of Dendrobium, 15-25 parts of Lithocarpus Polystachys Rehd Tea, 12-16 parts of fat sea cucumber, 6-10 parts of mango peel, 10-14 parts of cassia seed, 16-20 parts of wormwood leaf, 4-8 parts of magnolia officinalis and 14-18 parts of radish seed, which has the effects of clearing heat and resolving toxicity, invigorating stomach and food digestion, moistening intestines and defecation and promoting metabolism. It can be seen that in the prior art, Lithocarpus Polystachys Rehd Tea and Dendrobium are mainly mixed and crushed with traditional Chinese medicine to make a beverage, and there is no deep processing on the components, which leads to that the beverage is not obvious in targeting, the health care effect is not outstanding, and the practical value needs to be further developed. SUMMARY

[0004] In order to solve the problems in the prior art, the present application provides a kind of Lithocarpus Polystachys Rehd Tea and its preparation method, which improves the nutritional components of the beverage by targeted processing of Lithocarpus Polystachys Rehd Tea and Dendrobium, and strengthens the health care effect of the beverage. The specific technical scheme is as follows:

[0005] A Dendrobium candidum substitute tea is prepared from the following components in parts by weight: Dendrobium candidum 30-40 parts, Dendrobium 25-33 parts, papaya powder 20-23 parts, prepared rehmannia 10-20 parts, angelica 10-20 parts, white peony root 10-20 parts, isatis root 4-9 parts, forsythia 3-7 parts, mulberry leaf 5-10 parts;

[0006] The papaya powder is prepared as follows: separating the skin, flesh and seeds of papaya, and beating the papaya flesh into pulp for later use; frying the papaya skin and seeds in an iron pan at 100-110 DEG C for 10-15 min, and grinding into powder with a mesh size of 800-1000; mixing the powder with 3-5 times its mass of purified water, and adjusting the pH of the mixture to 3-3.5 with acetic acid, and standing at 35-37 DEG C for 30-37 min; mixing the pulp with the mixture, and stirring until uniform; diluting with water to 4-6 times the volume, and heating to 45-48 DEG C with a microwave; filtering the mixture with a microfilter, and collecting the filtrate for later use; diluting the residue with edible alcohol to 8-10 times the volume, and heating to 53-55 DEG C with a microwave, and standing for 15-23 min; filtering again, and combining the two filtrates; adjusting the pH to 6.5-6.8 with a sodium carbonate solution, and spray drying to obtain the papaya powder.

[0007] Further, the microwave power is 800-900 W.

[0008] Further, the mass fraction of the sodium carbonate solution is 0.11-0.15%.

[0009] The preparation method of the Dendrobium candidum substitute tea is as follows:

[0010] (1) preparing an amino acid extract

[0011] Mixing the Dendrobium candidum and Dendrobium, and placing them in a pottery jar; spraying the mixture with 8-11% of its mass of vinegar, and heating to 40-45 DEG C for 4-5 min; after cooling to room temperature, stirring for 2-3 min, and heating to 38-43 DEG C for 10-13 min; taking out the Dendrobium candidum and Dendrobium, and airing for 8-19 min; and placing them in a grinder to process into powder with a mesh size of 80-100.

[0012] Mixing the powder with the papaya powder, and adjusting the water content to 67-78%; adding lactic acid bacteria for anaerobic fermentation; placing in a freezer at minus 20-25 DEG C for 8-9 h; thawing at room temperature, and then concentrating and drying under reduced pressure; adding a citric acid-sodium citrate buffer solution to the dried material, and adjusting the pH to 4.9-5.1; standing at 48-51 DEG C for 15-17 min; using a shaker at 65-70 times / min for 30-35 min; reducing the temperature to 43-45 DEG C; and treating with ultrasonic waves for 23-27 min, and standing until clear.

[0013] The clear liquid is subjected to cation exchange resin, eluted with neutral eluent, and eluted with alkaline eluent, and the column effluent, the neutral eluent, and the alkaline eluent are centrifuged to obtain supernatant, which is subjected to ultrafiltration, concentration, and drying to obtain an extract;

[0014] (2) Preparation of tea powder

[0015] The rehmannia, angelica, white peony root, isatis root, forsythia, and mulberry leaves are mixed to process into 200-300 mesh powder, 5-8 times the mass of water is added, and the mixture is soaked for 20-30 min, heated to 38-40 DEG C, and subjected to ultrasonic treatment for 21-26 min, soaked for 80-90 min, filtered, the filter residue is diluted with 3-5 times the mass of water, heated to boiling, soaked for 13-18 min, filtered, the obtained filtrate is cooled to room temperature, combined with the aforementioned filtrate, and concentrated and dried under reduced pressure to obtain the extract.

[0016] Further, the solution flow rate of the cation exchange resin is 0.6-0.9 BV / h, and the loading amount is 3-5 BV.

[0017] Further, the neutral eluent is pure water, and the alkaline eluent is prepared by adjusting the pH of 65-68% ethanol solution to 8-8.3 with ammonia water.

[0018] Further, the hollow fiber membrane is used in the ultrafiltration.

[0019] Further, the anaerobic fermentation conditions are temperature 28-31 DEG C and time 25-27 h, and the amount of citric acid-sodium citrate buffer solution is 11-14 times the mass of the dried material.

[0020] Further, the ultrasonic power is 600-700 W.

[0021] Further, the total acid content of the vinegar is 4-5 mg / 100 ml.

[0022] Compared with the prior art, the technical effects of the present application are embodied in:

[0023] The Dendrobium officinale substitute tea prepared by the present application is rich in nutrients, has high amino acid content, contains various effective components such as minerals, flavones, and polysaccharides, has good taste, can promote the absorption of the body, improve the cell activity, improve the liver function, and has good protective effect on acute liver injury.

[0024] The present application uses papaya to prepare high-activity powder fermentation to form a bio-enzyme active ferment extract containing amino acid mixture, and then uses rehmannia to nourish the liver and blood and tonify kidney yin, strengthens liver absorption and metabolism; uses angelica to invigorate the blood and regulate menstruation and relieve pain to slow down the effect of external stimulation on the liver; uses white peony root to promote the use of tea to improve liver immunity; uses isatis root to promote the body tissue around the liver to improve metabolism and create an efficient detoxification cell environment for the liver; uses forsythia to promote liver blood flow, strengthen toxin elimination, inhibit the spread of diseased cells, and ensure the normal operation of liver function; uses mulberry leaves to improve cell activity, promote liver meridians, strengthen liver nutrient absorption, and improve liver immunity; through the overall combination of traditional Chinese medicine, promote the diffusion and absorption of amino acids in the body, strengthen the immune effect of liver cells, and promote the activation of liver cell self-repair function under external stimulation. DETAILED DESCRIPTION

[0025] The technical solutions of the present application will be further limited in combination with specific embodiments, but the scope of protection is not limited to the description.

[0026] Example 1

[0027] A gan tea and dendrobium substitute tea is composed of the following components by weight: gan tea 30 parts, dendrobium 25 parts, papaya powder 20 parts, rehmannia 10 parts, angelica 10 parts, white peony root 10 parts, isatis root 4 parts, forsythia 3 parts, and mulberry leaves 5 parts.

[0028] The preparation method of the gan tea and dendrobium substitute tea is as follows:

[0029] (1) Preparation of amino acid extract

[0030] Mix gan tea and dendrobium, put them into a pottery jar, spray a mixture of 8% vinegar, heat to 40℃ for 4min, cool to room temperature, stir for 2min, heat to 38℃ for 10min; take out the gan tea and dendrobium, spread and air for 8min, put them into a pulverizer, process into 80 mesh powder;

[0031] Mix the above powder with papaya powder, adjust the water content to 67%, add lactic acid bacteria for anaerobic fermentation, freeze at minus 20℃ for 8h, thaw at room temperature, then reduce pressure and dry, add citric acid-sodium citrate buffer solution to the dried material, adjust the pH to 4.9, stand at 48℃ for 15min, use a shaker at 65 times / min for 30min, reduce the temperature to 43℃, treat with ultrasonic wave for 27min, and stand for clarification; the anaerobic fermentation conditions are temperature 28℃ and time 25h; the amount of citric acid-sodium citrate buffer solution is 11 times the mass of the dried material; the ultrasonic wave power is 600W; the amount of lactic acid bacteria is 7% of the mass of the mixed powder;

[0032] The supernatant is collected by passing the supernatant through a cation exchange resin, eluted with a neutral eluent, and eluted with an alkaline eluent, and the column effluent, the neutral eluent, and the alkaline eluent are centrifuged to obtain the supernatant, which is subjected to ultrafiltration, concentration, and drying to obtain the extract;

[0033] The solution flow rate in the cation exchange resin is 0.9 BV / h, and the loading amount is 5 BV; the neutral eluent is pure water; the alkaline eluent is prepared by adjusting the pH of a 68% ethanol solution to 8.3 with ammonia water; and a hollow fiber membrane is used in the ultrafiltration;

[0034] The total acid content of the vinegar is 4 mg / 100 ml;

[0035] The preparation method of the papaya powder is as follows:

[0036] The papaya peel, flesh, and seeds are separated, and the papaya flesh is pulped for later use; the papaya peel and seeds are fried in an iron pot at 100°C for 10 min, and then pulverized into a powder with a particle size of 800 mesh; the powder is mixed with 3 times its mass of purified water, and the pH of the mixture is adjusted to 3 with acetic acid; the mixture is then placed at 35°C for 30 min; the fruit pulp and the mixture are mixed and stirred uniformly, and then diluted with water to 4 times its volume; the mixture is heated to 45°C with a microwave; the mixture is micro-filtered, and the filtrate is collected for later use; the residue is diluted with edible ethanol to 8 times its volume, heated to 53°C with a microwave, and maintained at this temperature for 15 min; the residue is micro-filtered again, and the filtrates from the two times of filtration are combined; the pH of the combined filtrate is adjusted to 6.5 with a sodium carbonate solution; and the mixture is spray-dried to obtain the papaya powder; the microwave power is 800 W; and the mass fraction of the sodium carbonate solution is 0.11%;

[0037] (2) Preparation of tea powder

[0038] The rehmannia, angelica, white peony root, isatis root, forsythia, and mulberry leaves are mixed and processed into a powder with a particle size of 200 mesh; 5 times the mass of the powder is added with purified water, and the mixture is soaked for 20 min; the mixture is heated to 38°C and treated with ultrasonic waves for 21 min; the mixture is soaked for 80 min, filtered, and the residue is diluted with purified water to 3 times its volume; the mixture is heated to boiling, soaked for 13 min, filtered, and the filtrate is cooled to room temperature; the filtrate is combined with the previously obtained filtrate, and the combined filtrate is concentrated and dried under reduced pressure; and the dried filtrate is mixed uniformly with the extract obtained in step (1).

[0039] Example 2

[0040] A kind of Dendrobium candidum substitute tea, which is composed of the following components by weight: Dendrobium candidum 40 parts, Dendrobium 33 parts, papaya powder 23 parts, rehmannia 20 parts, angelica 20 parts, white peony root 20 parts, isatis root 9 parts, forsythia 7 parts, and mulberry leaves 10 parts;

[0041] The preparation method of the Dendrobium candidum substitute tea is as follows:

[0042] (1) Preparation of amino acid extract

[0043] Mixing the Gan tea and the Dendrobium, putting them into a pottery jar, spraying the mixture with vinegar of 11% of the mass of the mixture, heating to 45℃ for 5min, cooling to room temperature, stirring for 3min, heating to 43℃ for 13min, taking out the Gan tea and the Dendrobium, airing for 19min, putting them into a pulverizer to process into powder of 100 mesh;

[0044] Mixing the powder with the papaya powder, adding water to adjust the water content to 8%, adding lactic acid bacteria for anaerobic fermentation, freezing at minus 25℃ for 9h, thawing at room temperature, drying by vacuum concentration, adding citric acid-sodium citrate buffer solution to the dried substance to adjust the pH to 5.1, standing at 51℃ for 17min, using a shaker to shake at 70 times / min for 35min, reducing the temperature to 45℃, treating with ultrasonic wave for 27min, standing to clarify; the amount of the lactic acid bacteria is 8% of the mass of the mixed powder;

[0045] Taking the supernatant through cation exchange resin, eluting with neutral eluent, then eluting with alkaline eluent, collecting the column effluent, neutral eluent and alkaline eluent, centrifuging to take the supernatant, then ultrafiltering, concentrating and drying to obtain the extract;

[0046] The solution flow rate in the cation exchange resin is 0.9BV / h, and the loading amount is 5BV; the neutral eluent is pure water; the alkaline eluent is prepared by adjusting the pH of 68% ethanol solution to 8.3 with ammonia water; hollow fiber membrane is used in the ultrafiltration;

[0047] The anaerobic fermentation conditions are temperature of 31℃ and time of 27h; the amount of the citric acid-sodium citrate buffer solution is 14 times of the mass of the dried substance; the ultrasonic wave power is 700W;

[0048] The total acid content of the vinegar is 5mg / 100ml;

[0049] The preparation method of the papaya powder is as follows:

[0050] Separating the papaya peel, pulp and seeds, beating the pulp for standby; frying the papaya peel and seeds in an iron pot at 110℃ for 15min, pulverizing into powder of 1000 mesh, mixing the powder with 5 times of pure water, adjusting the pH of the mixture to 3.5 with acetic acid, standing at 37℃ for 37min; mixing the pulp slurry with the mixture, stirring uniformly, diluting with water by 6 times, heating to 48℃ with microwave, microfiltering the mixture, collecting the filtrate for standby; diluting the residue with edible ethanol by 10 times, heating to 55℃ with microwave, standing for 23min, microfiltering again, combining the filtrates, adjusting the pH to 6.8 with sodium carbonate solution, and spray drying to obtain the papaya powder; the microwave power is 900W; the mass fraction of the sodium carbonate solution is 0.15%;

[0051] (2) Preparation of tea powder

[0052] Mix the rehmannia, angelica, white peony root, isatis root, forsythia, mulberry leaves into 300-mesh powder, add 8 times the mass of water, soak for 30 min, heat to 40℃, ultrasonic treatment for 26 min, soak for 90 min, filter, dilute the residue with water 5 times, heat to boiling, soak for 18 min, filter, combine the obtained filtrate with the previous filtrate after cooling to room temperature, concentrate and dry under reduced pressure, mix with the extract obtained in step (1) to obtain the tea powder.

[0053] Example 3

[0054] A Dendrocalamopsis oldhami substitute tea, which is composed of the following components in parts by weight: Dendrocalamopsis oldhami 35 parts, Dendrobium 28 parts, papaya powder 21 parts, rehmannia 19 parts, angelica 20 parts, white peony root 10 parts, isatis root 9 parts, forsythia 3 parts, mulberry leaves 10 parts.

[0055] The preparation method of the Dendrocalamopsis oldhami substitute tea is as follows:

[0056] (1) Preparation of amino acid extract

[0057] Mix the Dendrocalamopsis oldhami and Dendrobium, put them into a pottery jar, spray 9% of the mass of the mixture with vinegar, heat to 45℃ and stew for 4 min, cool to room temperature, stir for 3 min, heat to 38℃ and stew for 13 min, take out the Dendrocalamopsis oldhami and Dendrobium, spread and dry for 19 min, put them into a pulverizer and process into 80-mesh powder.

[0058] Mix the above powder with papaya powder, adjust the water content to 67% by adding water, anaerobically ferment with lactic acid bacteria, freeze at minus 25℃ for 8 h, thaw at room temperature, and then concentrate and dry under reduced pressure, add citric acid-sodium citrate buffer to the dried material, adjust the pH to 5.1, stand at 48℃ for 17 min, use a shaker to shake at 65 times / min for 35 min, reduce the temperature to 43℃, treat with ultrasonic waves for 27 min, and stand until clear; the amount of lactic acid bacteria used is 6% of the mass of the mixed powder.

[0059] Take the above clear liquid through a cation exchange resin, elute with a neutral eluent, and then elute with an alkaline eluent, centrifuge the column effluent, the neutral eluent, and the alkaline eluent to obtain the supernatant, and then obtain the extract through ultrafiltration, concentration, and drying;

[0060] The solution flow rate in the cation exchange resin is 0.88 BV / h, and the loading amount is 5 BV; the neutral eluent is pure water; the alkaline eluent is prepared by adjusting the pH of a 65% ethanol solution to 8.3 with ammonia water; and a hollow fiber membrane is used in the ultrafiltration;

[0061] The anaerobic fermentation condition is temperature 31℃, time 25h; the amount of the citric acid-sodium citrate buffer solution is 14 times of the dry matter mass; the ultrasonic power is 600W;

[0062] The total acid content of the vinegar is 5mg / 100ml;

[0063] The preparation method of the papaya powder is:

[0064] Separate the papaya peel, flesh and seeds, and beat the papaya flesh into pulp for standby; fry the papaya peel and seeds in an iron pot at 110℃ for 10min, crush into 1000 mesh powder with a crusher, mix the powder with 5 times of pure water in mass, adjust the pH of the mixture to 3 with acetic acid, and stand still at 37℃ for 30min; mix the pulp and the mixture, dilute with water by 6 times, heat to 45℃ with microwave, filter the mixture with microfiltration, collect the filtrate for standby; dilute the residue with edible alcohol by 10 times, heat to 53℃ with microwave, stand still for 23min, filter again, combine the filtrates, adjust the pH to 6.5 with sodium carbonate solution, and spray dry to obtain the papaya powder; the microwave power is 900W; the mass fraction of the sodium carbonate solution is 0.11%;

[0065] (2) Preparation of tea powder

[0066] Mix rehmannia, angelica, white peony root, isatis root, forsythia, and mulberry leaf into 300 mesh powder, add 5 times of clear water in mass, soak for 20min, heat to 40℃, treat with ultrasonic wave for 26min, soak for 80min, filter, dilute the residue with clear water by 3 times, heat to boiling, soak for 18min, filter, cool the obtained filtrate to room temperature, combine with the aforementioned filtrate, reduce pressure to concentrate and dry, and mix with the extract obtained in step (1) uniformly.

[0067] Comparative example setting:

[0068] Comparative example setting:

[0069]

[0070] Test example 1

[0071] Prepare the substitute tea according to examples 1-3 and comparative examples 1-5 respectively, determine the total amino acid content in step (1) by the ninhydrin method, and calculate the extraction rate of total amino acid.

[0072]

[0073] As can be seen from the table, according to the method of the present application, more amino acids can be extracted from the tea and dendrobium, while the comparative example 1 does not use vinegar to treat the tea and dendrobium, resulting in that the structure of the raw materials is not destroyed initially, and the subsequent extraction agent soaking efficiency is low, which affects the final extraction effect; the comparative example 2 does not use lactic acid bacteria fermentation, because the direct acid production of lactic acid bacteria can promote the dissolution of amino acids, and the reproduction of the bacteria can produce a large amount of amino acids, and the secretions of lactic acid bacteria can also promote the decomposition of the raw material organization, the amino acid extraction rate of the comparative example 2 without lactic acid bacteria is significantly lower than that of other comparative examples; the comparative example 3 is not frozen at low temperature, and the fermentation product loses the effect of freezing and crushing, the release of amino acids in the raw materials is not complete, resulting in low final amino acid extraction efficiency; the comparative example 4 does not roast papaya seeds and papaya skin, and the nutritional components cannot be completely released, resulting in low lactic acid bacteria reproduction efficiency and affecting the formation of amino acids; the comparative example 5 does not use ultrasonic treatment when preparing papaya powder, and the soluble nutritional components in the final papaya powder are reduced, which does not obviously promote the reproduction of lactic acid bacteria, resulting in low overall amino acid extraction efficiency.

[0074] Test Example 2

[0075] In order to further verify the effect of the present application on liver damage, the following comparative examples are added,

[0076]

[0077] Take 50 SPF male Kunming mice, weighing 19-21 g, according to the conventional feeding for one week, randomly divided into 10 groups, the number is consistent, respectively blank control group, example 1 group, comparative examples 1-7 group, liver damage model group. The blank control group and the liver damage model group use 0.1 ml / 10g dosage by gavage, the example 1 group, the comparative examples 1-7 group use the corresponding example 1, the comparative examples 1-7 medicine by gavage according to 0.1ml / 10g, once a day, for 10 days; 80min after the last day of medication, in the example 1 group, the comparative examples 1-7 group, the liver damage model group, use the mixture containing carbon tetrachloride and peanut oil (carbon tetrachloride content 5%) according to 0.1ml / 10g injection into the abdominal cavity of each group of mice, the blank group is injected with the same amount of normal saline, after injection, stop the mouse food supply, normal water supply, 24h after the mouse study blood separation serum, detection ALT activity, the results are as follows:

[0078]

[0079] As can be seen from the table, the 1ALT value of Example 1 group 1 using the drug of the present application is the lowest, the values of Comparative Examples 1-7 groups are significantly higher than that of Example 1 group, at the same time, the values of Comparative Examples 2 and 4 are obviously high, and are closest to the liver injury group; and Comparative Examples 6 and 7 directly lack the main components of forsythia and mulberry leaves, resulting in that the overall drug has a low effect on the nutrition absorption and toxin exclusion of liver cells, and the overall detection value is high.

[0080] The liver slices were taken from the mice in each group, fixed with 10% formaldehyde solution for 24h, dehydrated, treated by wax embedding, sliced and baked, and the damage of liver tissue in each experimental group was observed. The liver cells in the blank control group slice were arranged clearly and completely; the cells in the liver injury model group were arranged in disorder, the intercellular structure was not obvious, and the damage effect was obvious; relatively, the cells in Comparative Examples 1-7 groups were more clear and complete than those in the liver injury model group, and had a smaller damage effect. The cell slice of Example 1 group was more complete and clear, and was closest to the slice of the blank control group.

[0081] It can be seen that the Dendrobium nobile Lindl. of the present application has obvious protective effect on liver in liver cell damage, can promote liver cells to exert self-repairing effect, and reduce the liver damage effect caused by external environmental stimulation.

Claims

1. A Dendrobium candidum substitute tea, characterized in that, The tea is prepared from the following components by weight: 30-40 parts of Gantian, 25-33 parts of dendrobium, 20-23 parts of papaya powder, 10-20 parts of radix rehmanniae preparatae, 10-20 parts of angelica, 10-20 parts of radix paeoniae alba, 4-9 parts of radix isatidis, 3-7 parts of forsythia, and 5-10 parts of mulberry leaves; The papaya powder is prepared by the following steps: separating the skin, pulp and seeds of papaya, beating the pulp into pulp slurry, frying the skin and seeds in an iron pot at 100-110 DEG C for 10-15 min, crushing the fried skin and seeds into powder with a mesh size of 800-1000, mixing the powder with 3-5 times of pure water, adjusting the pH of the mixture to 3-3.5 with acetic acid, and standing the mixture at 35-37 DEG C for 30-37 min; mixing the pulp slurry with the mixture, stirring uniformly, diluting with water by 4-6 times, heating to 45-48 DEG C with a microwave, micro-filtering the mixture, collecting the filtrate, diluting the residue with edible alcohol by 8-10 times, heating to 53-55 DEG C with a microwave, micro-filtering again, combining the filtrates, adjusting the pH of the combined filtrate to 6.5-6.8 with a sodium carbonate solution, and spray drying to obtain the papaya powder. The preparation method of the Gantian and dendrobium tea comprises the following steps: (1) preparing amino acid extract mixing Gantian and dendrobium, spraying the mixture with 8-11% of vinegar, heating to 40-45 DEG C and stewing for 4-5 min, cooling to room temperature, stirring for 2-3 min, heating to 38-43 DEG C and stewing for 10-13 min, taking out the Gantian and dendrobium, airing for 8-19 min, and processing into powder with a mesh size of 80-100; mixing the powder with papaya powder, adjusting the water content to 67-78%, adding lactic acid bacteria for anaerobic fermentation, freezing at minus 20-25 DEG C for 8-9 h, thawing at room temperature, vacuum concentration and drying, adding citric acid-sodium citrate buffer solution to the dried product, adjusting the pH to 4.9-5.1, standing at 48-51 DEG C for 15-17 min, oscillating at 65-70 times / min for 30-35 min with a shaker, reducing the temperature to 43-45 DEG C, and treating with ultrasonic waves for 23-27 min, and standing to clarify; collecting the column effluent, the neutral eluent and the alkaline eluent, centrifuging to obtain the supernatant, and obtaining the extract through ultrafiltration, concentration and drying; (2) preparing tea powder mixing radix rehmanniae preparatae, angelica, radix paeoniae alba, radix isatidis, forsythia and mulberry leaves to process into powder with a mesh size of 200-300, adding 5-8 times of water, soaking for 20-30 min, heating to 38-40 DEG C, treating with ultrasonic waves for 21-26 min, soaking for 80-90 min, filtering, diluting the residue with water by 3-5 times, heating to boiling, soaking for 13-18 min, filtering, combining the obtained filtrate with the filtrate obtained in the above step, and mixing the extract obtained in step (1) uniformly.

2. The Dendrobium candidum substitute tea according to claim 1, characterized in that, The microwave power is 800-900 W.

3. The Dendrobium candidum substitute tea according to claim 1, characterized in that, The mass fraction of the sodium carbonate solution is 0.11-0.15%.

4. The Dendrobium candidum substitute tea according to claim 1, characterized in that, The solution flow rate in the cation exchange resin is 0.6-0.9 BV / h, and the loading amount is 3-5 BV.

5. The Dendrobium candidum substitute tea according to claim 1, characterized in that, The neutral eluent is pure water, and the preparation method of the alkaline eluent is as follows: 65-68% ethanol solution is adjusted to pH 8-8.3 by using ammonia water. 6.The Dendrobium candidum substitute tea according to claim 1, characterized by, The hollow fiber membrane is used in the ultrafiltration. 7.The Dendrobium candidum substitute tea according to claim 1, characterized in that, The anaerobic fermentation conditions are temperature 28-31 DEG C and time 25-27 h, and the amount of the citric acid-sodium citrate buffer is 11-14 times the dry matter mass. 8.The Dendrobium candidum substitute tea according to claim 1, characterized in that, The ultrasonic power is 600-700 W.

9. The preparation method of Dendrobium candidum substitute tea according to claim 1, characterized in that, The total acid content of the vinegar is 4-5 mg / 100 ml.

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