A digital holographic microscope

Abstract

The application discloses a kind of digital holographic microscopes, including laser light source and CCD sensor, reference light optical path and object light optical path are arranged between laser light source and CCD sensor, object light optical path is provided with stage in the described stage includes pedestal, pedestal is connected with top plate by lifting adjustment mechanism, lifting adjustment mechanism includes the first servo motor fixed on pedestal, the output of first servo motor is connected with first rack, second servo motor is fixed on first rack, the output of second servo motor is connected with second rack, second rack is fixedly connected with top plate, first servo motor drives first rack to move up and down, second servo motor drives second rack to move up and down, cavity is provided in top plate, glass slide rack is installed in cavity, glass slide is inserted in electromagnetic telescopic clamp.The application can improve the deficiencies of prior art, realize the fast holographic imaging of multiple samples.

Description

TECHNICAL FIELD

[0001] The present application relates to a kind of microscopic imaging equipment, especially a kind of digital holographic microscope. BACKGROUND

[0002] Digital holographic microscope is a kind of microscopic imaging equipment using the interference of laser to generate holographic image. Since the optical path parameters of generating holographic image require high accuracy, it needs to adjust the parameters of each element in optical path for a long time after each time replacing imaging sample, which leads to long adjustment time, directly affecting the efficiency of sample imaging. SUMMARY

[0003] The technical problem to be solved by the present application is to provide a kind of digital holographic microscope, which can solve the deficiencies of prior art and realize the rapid holographic imaging of multiple samples.

[0004] To solve the above technical problems, the technical solutions adopted by the present application are as follows.

[0005] A kind of digital holographic microscope, including laser light source and CCD sensor, reference light path and object light path are arranged between laser light source and CCD sensor, object light path is provided with stage, laser is emitted by laser light source and passes through reference light path and object light path respectively, and CCD sensor collects the interference image generated by two laser beams, the stage includes base, the top plate is connected to the base by lifting adjustment mechanism, the lifting adjustment mechanism includes the first servo motor fixed on the base, the output end of the first servo motor is connected with the first rack, the second servo motor is fixed on the first rack, the output end of the second servo motor is connected with the second rack, the second rack is fixedly connected with the top plate, the first servo motor drives the first rack to move up and down, the second servo motor drives the second rack to move up and down, the cavity is provided in the top plate, the slide rack is installed in the cavity, the spring is connected with the inner wall of the cavity, the adjusting cylinder is installed at the bottom of the cavity, the piston end of the adjusting cylinder is connected with the slide rack through the universal joint, a plurality of parallel insertion slots are arranged in the slide rack, an electromagnetic telescopic clamp is arranged at the edge of each insertion slot, the slide is inserted into the electromagnetic telescopic clamp, the electromagnetic telescopic clamp drives the slide to move left and right in the insertion slot, and the through hole and the sample placement area are arranged on the slide respectively.

[0006] Preferably, the slide rack is fixed with a seat corresponding to the spring, the spring is fixedly connected with the seat, the center of the seat is provided with a rubber column, and the rubber column is inserted into the spring and in sliding contact with the inner wall of the spring.

[0007] Preferably, a rubber sliding groove is arranged in the insertion slot, the edge of the slide is provided with a plug which is slidingly inserted into the rubber sliding groove, and high-resistance friction zones are arranged at both ends of the rubber sliding groove.

[0008] As preferred, the electromagnetic telescopic clamp comprises a telescopic driving body, a first connecting rod and a second connecting rod are installed in series on the telescopic driving body, the first connecting rod and the telescopic driving body and the first connecting rod and the second connecting rod are connected through torsional springs, and the free end of the second connecting rod is provided with a clamping body.

[0009] As preferred, the clamping body comprises a bottom clamp and a top clamp, the top clamp comprises a rubber inclined surface part and a rubber flat surface part connected in head-to-tail mode, the top surface of the slide glass is provided with a clamping groove matched with the rubber flat surface part, and the outer side end of the clamping groove is provided with an inclined slope matched with the rubber inclined surface part in pressure connection.

[0010] The beneficial effects brought by the above technical scheme are that: by using the newly designed object table, the present application can realize the one-time installation of multiple samples and the sequential imaging of different samples. Since the installation positions of the samples are known, the object table only needs to be adjusted, and other components in the optical path do not need to be adjusted, the focusing speed is fast, and thus the imaging efficiency of the samples is greatly improved. BRIEF DESCRIPTION OF DRAWINGS

[0011] Figure 1 is the optical path diagram of one specific embodiment of the present application.

[0012] Figure 2 is the structure diagram of the object table in one specific embodiment of the present application.

[0013] Figure 3 is the structure diagram of the inside of the cavity in one specific embodiment of the present application.

[0014] Figure 4 is the structure diagram of the rubber sliding groove in one specific embodiment of the present application.

[0015] Figure 5 is the structure diagram of the electromagnetic telescopic clamp in one specific embodiment of the present application.

[0016] Figure 6 is the structure diagram of the clamping body in one specific embodiment of the present application. DETAILED DESCRIPTION

[0017] REFERENCE Figures 1-6One specific embodiment of the present application comprises a laser light source 1 and a CCD sensor 2, a reference light path 3 and an object light path 4 are arranged between the laser light source 1 and the CCD sensor 2, and an object table 5 is arranged in the object light path 4, the laser light emitted by the laser light source 1 passes through the reference light path 3 and the object light path 4 respectively, and the CCD sensor 2 collects the interference image generated by the two beams of laser light, the object table 5 comprises a base 6, the base 6 is connected with a top plate 7 through a lifting adjustment mechanism, the lifting adjustment mechanism comprises a first servo motor 8 fixed on the base 6, a first rack 9 connected with the output end of the first servo motor 8, a second servo motor 10 fixed on the first rack 9, a second rack 11 connected with the output end of the second servo motor 10, and the second rack 11 is fixedly connected with the top plate 7, the first servo motor 8 drives the first rack 9 to move up and down, the second servo motor 10 drives the second rack 11 to move up and down, the top plate 7 is provided with a cavity 12, a glass slide holder 13 is installed in the cavity 12, the glass slide holder 13 is connected with the inner wall of the cavity 12 through a spring 14, an adjusting cylinder 15 is installed at the bottom of the cavity 12, the piston end of the adjusting cylinder 15 is connected with the glass slide holder 13 through a universal joint 16, a plurality of parallel insertion slots 17 are arranged in the glass slide holder 13, an electromagnetic telescopic clamp 18 is arranged at the edge of each insertion slot 17, a glass slide 19 is inserted into the electromagnetic telescopic clamp 18, the electromagnetic telescopic clamp 18 drives the glass slide 19 to move left and right in the insertion slot 17, and a through hole 20 and a sample placement area 21 are arranged on the glass slide 19.

[0018] The method for using the microscope of the present application is as follows: according to the initial setting requirements, the light path is adjusted, then the sample to be measured is placed on the glass slide 19, the glass slide 19 is inserted into the insertion slot 17, and the glass slide 19 is fixed by using the electromagnetic telescopic clamp 18. By controlling the electromagnetic telescopic clamp 18, the sample placement area 21 on the target glass slide is aligned with the laser light path, the through holes 20 of other glass slides are aligned with the laser light path, the first servo motor 8 and the second servo motor 10 are started to adjust the height and position of the target glass slide, and the driving directions of the first servo motor 8 and the second servo motor 10 are opposite, so that high-precision position adjustment can be realized. Then, the inclination angle of the target glass slide is adjusted by the adjusting cylinder 15, so that the CCD sensor 2 can collect a clear interference image. After the interference image is collected, the sample placement area 21 on the next target glass slide is aligned with the laser light path by controlling the electromagnetic telescopic clamp 18, the through hole 20 on the previous target glass slide is aligned with the laser light path, and then the height and inclination angle are adjusted in the above-mentioned manner, so that the next sample imaging can be performed. The present application can realize continuous imaging operation of the sample, and has high efficiency.

[0019] The slide rack 13 is fixed with the card seat 22 corresponding to the spring 14, the spring 14 is fixedly connected with the card seat 22, the center of the card seat 22 is provided with the rubber column 23, the rubber column 23 is inserted into the spring 14 and is in sliding contact with the inner wall of the spring 14. The rubber column 23 not only provides compression limit and expansion damping for the spring 14, but also effectively enhances the radial support stability of the spring 14.

[0020] The slot 17 is provided with the rubber sliding groove 24, the edge of the slide 19 is provided with the insertion piece 25 in sliding insertion with the rubber sliding groove 24, and the two ends of the rubber sliding groove 24 are provided with the high-resistance friction area 26. The electromagnetic telescopic clamp 18 comprises the telescopic driving body 27, the first connecting rod 28 and the second connecting rod 29 are connected in series on the telescopic driving body 27, the first connecting rod 28 and the telescopic driving body 27 and the first connecting rod 28 and the second connecting rod 29 are connected through the torsion spring 30, and the free end of the second connecting rod 29 is provided with the clamp body 31. The clamp body 31 comprises the bottom clamp 32 and the top clamp 33, the top clamp 33 comprises the rubber inclined surface part 34 and the rubber plane part 35 connected in head-to-tail mode, the top surface of the slide 19 is provided with the clamping groove 36 in clamping cooperation with the rubber plane part 35, and the outer side end of the clamping groove 36 is provided with the inclined slope 37 in pressing cooperation with the rubber inclined surface part 34. The slide 19 is in sliding insertion in the rubber sliding groove 24, the friction force provided by the rubber sliding groove 24 can be used to improve the moving stability of the slide 19, the high-resistance friction area 26 at the two ends is used to reduce the shaking of the slide in the acceleration and deceleration initial stage, and the connecting rods connected through the torsion springs can make the movement of the slide more stable. The top clamp 33 with the optimized structure cooperates with the top surface of the slide 19, can provide flexible buffering for the slide 19 while ensuring clamping stability, and further reduces the vibration of the slide 19. The slide driving structure of the application can realize accurate and stable slide driving, and avoid the problem that the imaging adjustment time is prolonged due to the position deviation of the slide.

[0021] The basic principle and main features of the application and the advantages of the application are shown and described above. It should be understood by those skilled in the art that the application is not limited by the above examples, and the above examples and descriptions in the specification are only to illustrate the principle of the application, and various changes and improvements can be made to the application without departing from the spirit and scope of the application, and these changes and improvements all fall within the scope of the application. The scope of protection of the application is defined by the appended claims and their equivalents.

Claims

1. A digital holographic microscope, comprising a laser light source (1) and a CCD sensor (2), a reference light path (3) and an object light path (4) are arranged between the laser light source and the CCD sensor, an object table (5) is arranged in the object light path, the laser light source emits laser light through the reference light path and the object light path respectively, the CCD sensor collects the interference image generated by the two beams of laser light, the object table comprises a base (6), the base is connected with a top plate (7) through a lifting adjusting mechanism, the lifting adjusting mechanism comprises a first servo motor (8) fixed on the base, the output end of the first servo motor is connected with a first rack (9), a second servo motor (10) is fixed on the first rack, the output end of the second servo motor is connected with a second rack (11), and the second rack is fixedly connected with the top plate; characterized in that: a cavity (12) is arranged in the top plate, a slide rack (13) is mounted in the cavity, the slide rack is connected with the inner wall of the cavity through a spring (14), an adjusting cylinder (15) is mounted at the bottom of the cavity, and the piston end of the adjusting cylinder is connected with the slide rack through a universal joint (16); a clamping seat (22) corresponding to the spring is fixed on the slide rack, the spring is clamped and fixed with the clamping seat, a rubber column (23) is arranged at the center of the clamping seat and is inserted into the spring and in sliding contact with the inner wall of the spring; a plurality of parallel insertion grooves (17) are arranged in the slide rack, an electromagnetic telescopic clamp (18) is arranged at the edge of each insertion groove, a slide glass (19) is inserted into the electromagnetic telescopic clamp, the electromagnetic telescopic clamp drives the slide glass to move left and right in the insertion groove, and a through hole (20) and a sample placement area (21) are arranged on the slide glass respectively; the sample placement area on the target slide glass is aligned with the laser path and the through holes (20) of other slide glasses are aligned with the laser path by controlling the electromagnetic telescopic clamp; the electromagnetic telescopic clamp comprises a telescopic driving body (27), a first connecting rod (28) and a second connecting rod (29) are connected in series on the telescopic driving body (27), the first connecting rod and the telescopic driving body and the first connecting rod and the second connecting rod are connected through torsional springs (30), and a clamping body (31) is mounted at the free end of the second connecting rod (29); the clamping body comprises a bottom clamp (32) and a top clamp (33), the top clamp comprises a rubber inclined surface part (34) and a rubber flat surface part (35) connected in series, a clamping groove (36) matched with the rubber flat surface part (35) is arranged on the top surface of the slide glass, and a slope (37) matched with the rubber inclined surface part (34) is arranged at the outer side end of the clamping groove. A rubber sliding groove (24) is arranged in the insertion groove (17), an insertion piece (25) is arranged at the edge of the slide glass (19) and is slidably inserted into the rubber sliding groove (24), and high-resistance friction areas (26) are arranged at the two ends of the rubber sliding groove (24).

2. The digital holographic microscope according to claim 1, characterized in that: ​

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