Device and method for automatically fine-tuning coaxial arrangement of capillaries in a microfluidic chip

Abstract

The present invention belongs to the field of microfluidic chip technology, and discloses an automated fine-tuning microfluidic chip capillary coaxial arrangement device and method, comprising a base, a moving system, a fine-tuning system, an image system, and a control system, wherein the moving system, the fine-tuning system, and the image system are arranged on the base, the moving system and the fine-tuning system are at the same horizontal position, and the image system is arranged at the rear of the moving system. The present invention realizes the precise three-dimensional coaxial arrangement of the injection capillary and the collection capillary, and realizes low-cost, non-manual assembly of glass capillary microfluidic chips, ensuring the precise positioning and stable operation of the microfluidic chip during the assembly process, thereby improving the reliability and production efficiency of the microfluidic chip and reducing production costs. In addition, the microfluidic chip can be customized and assembled according to actual applications, further expanding the application range of the microfluidic chip.

Description

Technical Field

[0001] The present invention belongs to the technical field of microfluidic chips, and in particular relates to a device and method for automatically fine-tuning the coaxial arrangement of capillaries in a microfluidic chip. Background Art

[0002] Droplet microfluidics uses microchannels to manipulate immiscible fluids at the micrometer scale to form micron- or nanometer-sized droplets, with excellent controllability. Microchannels capable of precisely manipulating tiny fluids are called microfluidic chips or microchannel devices. Droplet microfluidics exploits the interfacial tension of fluids to form emulsions. The size of the droplets depends on the flow rate and properties of the fluids, as well as the size and structure of the microchannel device. Droplet microfluidics simplifies and efficiently prepares monodisperse and structurally controllable emulsions and microcapsules, and is widely used in drug delivery, biotemplating, cell culture, and microreactors.

[0003] Glass, with its excellent mechanical strength, light transmittance, and resistance to organic solvent corrosion, is also inexpensive and is currently the most widely used material for microfluidic chips. Microfluidic chips utilize glass capillaries as the functional units for droplet generation and collection. Hollow glass capillaries of varying thicknesses are typically coaxially nested. This coaxially nested microchannel structure enables the preparation of multiple emulsion microdroplets with a core-shell structure. However, manual assembly of microfluidic chips poses challenges, such as the inability to precisely coaxially align the injection and collection capillaries and the fragility of the circular capillary tips.

[0004] During the manual assembly of microfluidic chips, the tiny size of the glass capillaries makes it difficult to ensure the precise positioning and coaxial alignment of the sample and collection capillaries, resulting in inconsistent capillary alignment or deviation from their original path. Furthermore, manual assembly is significantly affected by environmental conditions, such as operator hand tremors and ambient vibrations, which can affect the accuracy of capillary assembly, leading to variations in the quality and performance of each microfluidic chip. If the coaxial positioning of the sample and collection capillaries on a microfluidic chip deviates, the core material in the sample capillary cannot be successfully encapsulated by the shell material, thus affecting the production efficiency and quality of the microcapsules. Summary of the Invention

[0005] The present invention aims to provide an apparatus and method for automatically fine-tuning the coaxial arrangement of capillaries in a microfluidic chip to solve the above-mentioned technical problems.

[0006] To solve the above technical problems, the specific technical solutions of the present invention for an automated fine-tuning microfluidic chip capillary coaxial arrangement device and method are as follows:

[0007] An automated fine-tuning microfluidic chip capillary coaxial arrangement device comprises a base, a moving system, a fine-tuning system, an imaging system, and a control system. The moving system and the fine-tuning system are mounted on the base and are on the same horizontal line. The imaging system is located on the back and above the moving system. The control system is electrically connected to the moving system, the fine-tuning system, and the imaging system. The moving system is used to place and move the microfluidic chip; the fine-tuning system is used to fix and move the injection capillary; the imaging system is used to observe the coaxial arrangement of the injection capillary and the collection capillary in the X, Y, and Z directions; and the control system is used to control the operating status of each system and to control the opening and closing of each component of the system.

[0008] Furthermore, the mobile system includes a mobile system support seat, a mobile platform, a device platform and a clip. The mobile system support seat is fixedly mounted on the base, and the mobile platform is fixedly mounted on the upper end of the mobile system support seat to adjust the position of the microfluidic chip. The mobile platform includes an X-direction mobile differential head, an X-direction mobile panel, a Y-direction mobile differential head, a Y-direction mobile panel, a Z-direction mobile differential head, and a Z-direction mobile panel. The X-direction mobile panel, the Y-direction mobile panel, and the Z-direction mobile panel are horizontally overlapped and connected by a threaded structure. The X-direction mobile differential head is arranged on one side of the X-direction mobile panel. The X-direction mobile differential head knob is adjusted to drive the mobile platform to move horizontally in the X direction through a single guide rail structure to adjust the movement of the microfluidic chip in the X direction. The Y-direction mobile panel is placed horizontally and the ... The mobile differential head is arranged on one side of the Y-direction moving panel. By turning the Y-direction moving differential head knob, the mobile platform is driven to move horizontally in the Y direction through the single guide rail structure to adjust the movement of the microfluidic chip in the Y direction. The Z-direction moving differential head is arranged on one side of the Z-direction moving panel. By adjusting the Z-direction moving differential head knob, the mobile platform is driven to move horizontally in the Z direction through the single guide rail structure to adjust the movement of the microfluidic chip in the Z direction. The X-direction moving differential head, Y-direction moving differential head, and Z-direction moving differential head are all electric differential heads. A driving device is provided inside the X-direction moving panel, Y-direction moving panel, and Z-direction moving panel, which is connected to the control system. The device platform is fixed on the upper side of the Z-direction moving panel for placing the microfluidic chip, and the clip is fixed on the upper side of the device platform for fixing the microfluidic chip.

[0009] Furthermore, the fine-tuning system includes a fine-tuning system support seat, a fine-tuning platform and a clamping device, the fine-tuning system support seat is fixedly mounted on the base, the fine-tuning platform is fixed on the fine-tuning system support seat, and is used to fine-tune the displacement of the sampling glass capillary, the fine-tuning platform includes an X-direction fine-tuning head, an X-direction fine-tuning panel, a Y-direction fine-tuning head, a Y-direction fine-tuning panel, a Z-direction fine-tuning head, a Z-direction fine-tuning panel, and a Z-direction panel support seat, the X-direction fine-tuning panel and the Y-direction fine-tuning panel are horizontally overlapped and connected by a threaded structure, the Z-direction panel support seat is an inverted T-shaped structure, the bottom of which is fixed above the Y-direction fine-tuning panel, the Z-direction fine-tuning panel is fixed to one side of the Z-direction panel support seat, the X-direction fine-tuning head is arranged on one side of the X-direction fine-tuning panel, and the X-direction fine-tuning head knob is adjusted to drive the fine-tuning platform along the Y-direction panel through the single guide rail structure. The X-direction fine-tuning micrometer head is arranged on one side of the Y-direction fine-tuning panel. The Y-direction fine-tuning micrometer head knob is adjusted to drive the fine-tuning platform to move horizontally in the Y direction through the single guide rail structure to adjust the movement of the injection capillary in the Y direction. The Z-direction fine-tuning micrometer head is arranged on one side of the Z-direction fine-tuning panel. The Z-direction fine-tuning micrometer head knob is adjusted to drive the fine-tuning platform to move horizontally in the Z direction through the single guide rail structure to adjust the movement of the injection capillary in the Z direction. The X-direction fine-tuning micrometer head, the Y-direction fine-tuning micrometer head, and the Z-direction fine-tuning micrometer head are all electric fine-tuning micrometer heads. A driving device is provided inside the X-direction fine-tuning panel, the Y-direction fine-tuning panel, and the Z-direction fine-tuning panel, which is connected to the control system. The clamping device is fixed to one side of the Z-direction fine-tuning panel by screws. The clamping device is provided with a clamping device for fixing the injection capillary.

[0010] Furthermore, the image system includes an image system rear plate, an image system upper plate, camera 1, and camera 2. The image system rear plate is vertically connected to the base, and a small hole is provided in the middle for fixing camera 1. Camera 1 is used to observe the coaxial arrangement of the injection capillary and the collection capillary in the Z direction. The image system upper plate is vertically connected to the image system rear plate, and a small hole is provided at one end for fixing camera 2. Camera 2 is used to observe the coaxial arrangement of the injection capillary and the collection capillary in the X and Y directions.

[0011] Furthermore, the control system includes a center console, a touch screen, an indicator light, a display screen, an operating keyboard and a switch. The touch screen is arranged on the left side of the center console for turning on and off the system operation status; the indicator light is arranged on the middle platform of the center console for issuing signal prompts; the display screen is arranged on the upper part of the center console for observing the three-dimensional coaxial arrangement position of the injection capillary and the collection capillary; the operating keyboard is arranged on the middle platform of the center console for inputting control instructions; the switch is arranged on the right side of the center console for turning on and off the center console; the control system drives and controls the displacement of the moving system through the moving module, and drives and controls the displacement of the fine-tuning system through the fine-tuning module.

[0012] The present invention also discloses a method for preparing a microfluidic chip using an automated fine-tuning microfluidic chip capillary coaxial arrangement device, comprising the following steps:

[0013] Step 1: Make the injection capillary and the collection capillary;

[0014] Step 2: Place a glass slide on the platform of the device and fix it with a clip. Insert the collection capillary from one side of the connecting capillary. Both are fixed to the surface of the glass slide with adhesive. The interface between the collection capillary and the connecting capillary is connected and fixed with an external phase dispensing needle. The collection capillary constitutes an external phase microchannel. The connecting capillary is a circular capillary. The obtained injection capillary is placed in a fixed position of the clamping device and the knob of the clamping device is turned to fix it.

[0015] Step 3: Turn on the center console and enter the motion system program on the center console to set the control program. The operator turns on the system start button through the touch screen. The motion control module receives the motion command and drives the motion system to adjust the X-direction motion micrometer head, Y-direction motion micrometer head, and Z-direction motion micrometer head to the initial position. Then, the fine-tuning control module receives the fine-tuning command and drives the fine-tuning system to adjust the X-direction fine-tuning micrometer head, Y-direction fine-tuning micrometer head, and Z-direction fine-tuning micrometer head to the initial position.

[0016] Step 4: Cameras 1 and 2 capture images of the microfluidic chip interior. Built-in displacement sensors determine the positional parameters (x, y, z) of the injection and collection capillaries and transmit these parameters to the control system. An image processing algorithm identifies the positional parameters of the injection and collection capillaries and calculates the offset.

[0017] Step 5: Based on the position parameter information provided by the imaging system, the control system generates a fine-tuning instruction, which is sent to the fine-tuning system through the fine-tuning control module. The X-direction fine-tuning micrometer head, the Y-direction fine-tuning micrometer head, and the Z-direction fine-tuning micrometer head respectively execute the instruction to adjust the injection capillary in the X, Y, and Z directions, so that the injection capillary enters from the other side of the connecting capillary and the injection capillary and the collection capillary are both within the image capture range of the imaging system;

[0018] Step 6: The imaging system captures real-time images of the adjusted positions of the injection and collection capillaries and performs position verification. If the injection and collection capillaries are coaxially aligned, the control system sends a completion signal and saves the configuration data. If deviations still exist, the control system repeats the fine-tuning control steps until the injection and collection capillaries achieve three-dimensional coaxial alignment.

[0019] Step 7: When the injection capillary and the collection capillary are in a three-dimensional coaxial arrangement, the interface between the injection capillary and the connecting capillary is fixed with an adhesive through an intermediate phase dispensing needle, and the injection capillary forms an inner phase microchannel.

[0020] Furthermore, step 1 includes: using a needle puller to process a circular glass capillary into two capillaries with a length of 5 cm and a tapered end, as an injection capillary and a collection capillary, the inner diameter of the tapered tip of the injection capillary is 30 μm, and the inner diameter of the tapered tip of the collection capillary is 100 μm, the obtained injection capillary and collection capillary are cleaned and dried to remove residual fine glass fragments, and the injection capillary and collection capillary are hydrophobic treated with octadecyltrichlorosilane, and then cleaned with ethanol and dried for standby use.

[0021] The present invention provides an automated, fine-tuned, coaxial capillary arrangement device and method for microfluidic chips. The device achieves precise three-dimensional coaxial alignment of sample and collection capillaries, enabling low-cost, manual assembly of glass capillary microfluidic chips. This ensures precise positioning and stable operation of the microfluidic chip during assembly, thereby improving the reliability and production efficiency of the microfluidic chip and reducing production costs. Furthermore, the microfluidic chip can be customized for specific applications, further expanding its application range. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the main structure of an automated fine-tuning microfluidic chip capillary coaxial arrangement device according to the present invention;

[0023] Figure 2 This is a schematic diagram of the main structure of the control system of the present invention;

[0024] Explanation of the symbols in the figure: 1. Base; 2. Moving system; 21. Moving system support base; 22. Moving platform; 221. X-direction moving micrometer head; 222. X-direction moving panel; 223. Y-direction moving micrometer head; 224. Y-direction moving panel; 225. Z-direction moving micrometer head; 226. Z-direction moving panel; 23. Device platform; 24. Clip; 3. Fine-tuning system; 31. Fine-tuning system support base; 32. Fine-tuning platform; 321. X-direction fine-tuning micrometer head; 322. X-direction fine-tuning surface Board; 323, Y-direction fine-tuning micrometer head; 324, Y-direction fine-tuning panel; 325, Z-direction fine-tuning micrometer head; 326, Z-direction fine-tuning panel; 327, Z-direction panel support seat; 33, clamping device; 331, pressing device; 4, image system; 41, image system rear panel; 42, image system upper panel; 43, camera 1; 44, camera 2; 5, control system; 51, center console; 52, touch screen; 53, indicator light; 54, display screen; 55, operation keyboard; 56, switch. DETAILED DESCRIPTION

[0025] In order to better understand the purpose, structure and function of the present invention, the following is a further detailed description of an automatic fine-tuning microfluidic chip capillary coaxial arrangement device and method of the present invention in conjunction with the accompanying drawings.

[0026] like Figure 1 Figure 2 As shown, an automated fine-tuning microfluidic chip capillary coaxial arrangement device of the present invention includes a base 1, a moving system 2, a fine-tuning system 3, an imaging system 4, and a control system 5. The moving system 2 and the fine-tuning system 3 are on the same horizontal line, and the imaging system 4 is located on the back and above the moving system 2. The control system 5 is independently arranged outside the other components of the microfluidic chip capillary coaxial arrangement device and is electrically connected to the moving system 2, the fine-tuning system 3, and the imaging system 4. The moving system 2 is used to place and move the microfluidic chip; the fine-tuning system 3 is used to fix and move the injection capillary; the imaging system 4 is used to observe the coaxial arrangement of the injection capillary and the collection capillary in the X, Y, and Z directions; the control system 5 is used to control the operating status of each system and control the opening and closing of each component of the system.

[0027] The mobile system 2 includes a mobile system support base 21, a mobile platform 22, a device platform 23, and a clip 24. The mobile system support base 21 is fixedly installed on the base 1, and the mobile platform 22 is fixed to the upper end of the mobile system support base 21 by screw connection, and is used to adjust the position of the microfluidic chip. It includes an X-direction mobile differential head 221, an X-direction mobile panel 222, a Y-direction mobile differential head 223, a Y-direction mobile panel 224, a Z-direction mobile differential head 225, and a Z-direction mobile panel 226. The X-direction mobile panel 222, the Y-direction mobile panel 224, and the Z-direction mobile panel 226 are placed horizontally and overlapped, and are all connected by a threaded structure. The X-direction mobile differential head 221 is set on one side of the X-direction mobile panel 222, and the X-direction mobile differential head 223 is adjusted. The 1 knob drives the mobile platform 22 to move in the X direction via a single guide rail structure (a single-track moving structure of the prior art, not described in detail here), thereby adjusting the movement of the microfluidic chip in the X direction. The Y-direction moving differential head 223 is located on the side of the Y-direction moving panel 224. Turning the Y-direction moving differential head 223 knob drives the mobile platform 22 in the Y direction via the single guide rail structure, thereby adjusting the movement of the microfluidic chip in the Y direction. The Z-direction moving differential head 225 is located on the side of the Z-direction moving panel 226. Adjusting the Z-direction moving differential head 225 knob drives the mobile platform 22 in the Z direction via the single guide rail structure, thereby adjusting the movement of the microfluidic chip in the Z direction. The X-direction moving differential head 221, the Y-direction moving differential head 223, and the Z-direction moving differential head 225 are all motorized differential heads. The X-direction moving panel 222, the Y-direction moving panel 224, and the Z-direction moving panel 226 are internally equipped with drive devices and are connected to the control system 5. The device platform 23 is fixed to the upper side of the Z-direction movable panel 226 by screw connection for placing the microfluidic chip, and the clip 24 is fixed to the upper side of the device platform 23 for fixing the microfluidic chip.

[0028] The fine-tuning system 3 includes a fine-tuning system support seat 31, a fine-tuning platform 32, and a clamping device 33. The fine-tuning system support seat 31 is fixedly mounted on the base 1, and the fine-tuning platform 32 is fixed to the fine-tuning system support seat 31 by screw connection, and is used to fine-tune the displacement of the sampling glass capillary. The fine-tuning platform 32 includes an X-direction fine-tuning head 321, an X-direction fine-tuning panel 322, a Y-direction fine-tuning head 323, a Y-direction fine-tuning panel 324, a Z-direction fine-tuning head 325, a Z-direction fine-tuning panel 326, and a Z-direction panel support seat 327. The X-direction fine-tuning panel 322 and the Y-direction fine-tuning panel 324 are placed horizontally and overlapped, and the two are connected by a threaded structure. The Z-direction panel support seat 327 is an inverted T-shaped structure, with the bottom fixed above the Y-direction fine-tuning panel 324, and the Z-direction fine-tuning panel 326 is fixed to the Z-direction panel support seat. On one side of the seat 327, the X-direction fine-tuning micrometer head 321 is set on one side of the X-direction fine-tuning panel 322. By adjusting the knob of the X-direction fine-tuning micrometer head 321, the fine-tuning platform 32 is driven to move horizontally along the X direction through the single guide rail structure to adjust the movement of the injection capillary in the X direction. The Y-direction fine-tuning micrometer head 323 is set on one side of the Y-direction fine-tuning panel 324. By adjusting the knob of the Y-direction fine-tuning micrometer head 323, the fine-tuning platform 32 is driven to move horizontally along the Y direction through the single guide rail structure to adjust the movement of the injection capillary in the Y direction. The Z-direction fine-tuning micrometer head 325 is set on one side of the Z-direction fine-tuning panel 326. By adjusting the knob of the Z-direction fine-tuning micrometer head 325, the fine-tuning platform 32 is driven to move horizontally along the Z direction through the single guide rail structure to adjust the movement of the injection capillary in the Z direction. The X-, Y-, and Z-direction fine-tuning heads 321, 323, and 325 are all motorized. The X-, Y-, and Z-direction fine-tuning panels 322, 324, and 326 are equipped with internal drive mechanisms and connected to the control system 7. The clamping device 33 is screwed to one side of the Z-direction fine-tuning panel 326 and includes a clamping device 331 for securing the sample injection capillary.

[0029] The imaging system 4 includes an imaging system rear panel 41, an imaging system upper panel 42, a first camera 43, and a second camera 44. The imaging system rear panel 41 is vertically connected to the base 1 and has a small hole in the middle for securing the first camera 43. The first camera 43 is used to observe the coaxial arrangement of the injection capillary and the collection capillary in the Z direction. The imaging system upper panel 42 is vertically connected to the imaging system rear panel 41 and has a small hole at one end for securing the second camera 44. The second camera 44 is used to observe the coaxial arrangement of the injection capillary and the collection capillary in the X and Y directions.

[0030] The control system 5 includes a central console 51, a touch screen 52, an indicator light 53, a display screen 54, an operating keyboard 55, and a switch 56. The touch screen 52 is arranged on the left side of the central console 51 and is used to turn the system operation status on and off. A manual operating system is further provided, which can be used to manually adjust the displacement of the mobile system 2 and the fine-tuning system. The indicator light 53 is arranged on the middle platform of the central console 51 and is used to send signal prompts. The display screen 54 is arranged on the upper part of the central console and is used to observe the three-dimensional coaxial arrangement position of the injection capillary and the collection capillary. The operating keyboard 55 is arranged on the middle platform of the central console 51 and is used to input control instructions. The switch 56 is arranged on the right side of the central console 51 and is used to turn the central console 51 on and off. The control system drives and controls the displacement of the mobile system 2 through the mobile module, and drives and controls the displacement of the fine-tuning system through the fine-tuning module.

[0031] The method of using an automated fine-tuning microfluidic chip capillary coaxial arrangement device for preparing a microfluidic chip of the present invention comprises the following steps:

[0032] Step 1: Use a needle puller to process a circular glass capillary into two 5 cm long capillaries with a tapered end. These serve as the injection and collection capillaries. The inner diameter of the tapered tip of the injection capillary is 30 μm, and the inner diameter of the tapered tip of the collection capillary is 100 μm. The resulting injection and collection capillaries are cleaned and dried to remove any remaining glass fragments. They are then treated with octadecyltrichlorosilane for hydrophobicity, rinsed with ethanol, and air-dried for later use.

[0033] Step 2: Place a glass slide on the device platform 23 and secure it with a clip 24. Insert the collection capillary from one side of the connecting capillary. Both are fixed to the glass slide surface with adhesive. The interface between the collection capillary and the connecting capillary is connected and fixed with an external phase dispensing needle. The collection capillary forms the external phase microchannel. The connecting capillary is a circular capillary. Place the resulting injection capillary in a fixed position on the clamping device 33 and secure it by turning the knob of the clamping device 331.

[0034] Step 3: Turn on switch 56 on the central console 51 and enter the motion system program on the central console 51 to set the control program. The operator activates the system start button via touch screen 52. The motion control module receives the motion command and drives the motion system 2 to adjust the X-direction micrometer head 221, the Y-direction micrometer head 223, and the Z-direction micrometer head 225 to their initial positions. The fine-tuning control module then receives the fine-tuning command and drives the fine-tuning system 3 to adjust the X-direction micrometer head 321, the Y-direction micrometer head 332, and the Z-direction micrometer head 325 to their initial positions.

[0035] Step 4: Camera 1 43 and Camera 2 44 capture images of the microfluidic chip interior. Built-in displacement sensors are used to obtain the position parameters (x, y, z) of the injection and collection capillaries. These position parameters are then transmitted to the control system 5. An image processing algorithm identifies the position parameters of the injection and collection capillaries and calculates the offset.

[0036] Step 5: Based on the position parameter information provided by the imaging system, the control system generates fine-tuning instructions and sends them to the fine-tuning system 3 via the fine-tuning control module. The X-direction fine-tuning micrometer head 221, the Y-direction fine-tuning micrometer head 223, and the Z-direction fine-tuning micrometer head 225 respectively execute the instructions to adjust the injection capillary in the X, Y, and Z directions, allowing the injection capillary to enter from the other side of the connecting capillary, so that the injection capillary and the collection capillary are both within the image capture range of the imaging system.

[0037] Step 6: Imaging system 4 captures real-time images of the adjusted positions of the injection and collection capillaries and verifies their alignment. If the injection and collection capillaries are coaxially aligned, control system 5 signals completion and saves the configuration data. If deviations persist, control system 5 repeats the fine-tuning control steps until the injection and collection capillaries achieve three-dimensional coaxial alignment.

[0038] Step 7: When the injection capillary and the collection capillary are in a three-dimensional coaxial arrangement, the interface between the injection capillary and the connecting capillary is fixed with an adhesive through an intermediate phase dispensing needle, and the injection capillary forms an inner phase microchannel.

[0039] It will be understood that the present invention is described by way of some embodiments, and it will be appreciated by those skilled in the art that various changes or equivalent substitutions may be made to these features and embodiments without departing from the spirit and scope of the present invention. In addition, under the teachings of the present invention, these features and embodiments may be modified to adapt to specific circumstances and materials without departing from the spirit and scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are intended to be protected by the present invention.

Claims

1. An automated fine-tuning microfluidic chip capillary coaxial arrangement device, comprising a base (1), a moving system (2), a fine-tuning system (3), an imaging system (4) and a control system (5), characterized in that: The moving system (2) and the fine-tuning system (3) are installed on the base (1) and are on the same horizontal line. The image system (4) is located on the back and above the moving system (2). The control system (5) is electrically connected to the moving system (2), the fine-tuning system (3), and the image system (4). The moving system (2) is used to place and move the microfluidic chip; the fine-tuning system (3) is used to fix and move the injection capillary; the image system (4) is used to observe the coaxial arrangement of the injection capillary and the collection capillary in the X, Y, and Z directions; the control system (5) is used to control the operating status of each system and control the opening and closing of each component of the system (5); the moving system (2) includes a moving system support base (21), a moving platform (22), a device platform (23), and a clamp (24). The moving system support base (21) is fixedly installed on the base The base (1) is provided with a movable platform (22) fixedly mounted on the upper end of the movable system support base (21), including a three-axis movable micrometer head for adjusting the position of the microfluidic chip, the device platform (23) is fixed on the upper side of the movable platform (22) for placing the microfluidic chip, and the clamp (24) is fixed on the upper side of the device platform (23) for fixing the microfluidic chip; the fine-tuning system (3) includes a fine-tuning system support base (31), a fine-tuning platform (32) and a clamping device (33), the fine-tuning system support base (31) is fixedly mounted on the base (1), the fine-tuning platform (32) is fixed on the fine-tuning system support base (31), including a three-axis fine-tuning micrometer head for fine-tuning the displacement of the injection capillary, the clamping device (33) is fixed on one side of the fine-tuning platform (32), and the clamping device (33) is provided with a pressing device (331) for fixing the injection capillary.

2. The automated fine-tuning microfluidic chip capillary coaxial arrangement device according to claim 1, characterized in that: The movable platform (22) includes an X-direction movable differential head (221), an X-direction movable panel (222), a Y-direction movable differential head (223), a Y-direction movable panel (224), a Z-direction movable differential head (225), and a Z-direction movable panel (226). The X-direction movable panel (222), the Y-direction movable panel (224), and the Z-direction movable panel (226) are horizontally overlapped and connected to each other through a threaded structure. The X-direction movable differential head (221) is arranged on one side of the X-direction movable panel (222). The knob of the X-direction movable differential head (221) is adjusted to drive the movable platform (22) to move horizontally in the X direction through the single guide rail structure to adjust the movement of the microfluidic chip in the X direction. The Y-direction movable differential head (223) is arranged on one side of the Y-direction movable panel (224). The Y-direction movable differential head (223) is rotated to rotate the Y-direction movable panel (224). The Z-direction moving differential head (223) knob is turned to drive the moving platform (22) to move in the Y direction through a single guide rail structure to adjust the movement of the microfluidic chip in the Y direction. The Z-direction moving differential head (225) is arranged on one side of the Z-direction moving panel (226). The Z-direction moving differential head (225) knob is adjusted to drive the moving platform (22) to move in the Z direction through a single guide rail structure to adjust the movement of the microfluidic chip in the Z direction. The X-direction moving differential head (221), the Y-direction moving differential head (223), and the Z-direction moving differential head (225) are all electric differential heads. A driving device is provided inside the X-direction moving panel (222), the Y-direction moving panel (224), and the Z-direction moving panel (226), which are connected to the control system (5). The device platform (23) is fixed on the upper side of the Z-direction moving panel (226).

3. The automated fine-tuning microfluidic chip capillary coaxial arrangement device according to claim 1, characterized in that: The fine-tuning platform (32) includes an X-direction fine-tuning head (321), an X-direction fine-tuning panel (322), a Y-direction fine-tuning head (323), a Y-direction fine-tuning panel (324), a Z-direction fine-tuning head (325), a Z-direction fine-tuning panel (326), and a Z-direction panel support seat (327). The X-direction fine-tuning panel (322) and the Y-direction fine-tuning panel (324) are placed horizontally and overlapped, and the two are connected by a threaded structure. The Z-direction panel support seat The support seat (327) is an inverted T-shaped structure, with the bottom fixed above the Y-direction fine-tuning panel (324), the Z-direction fine-tuning panel (326) is fixed to one side of the Z-direction panel support seat (327), and the X-direction fine-tuning micrometer head (321) is set on one side of the X-direction fine-tuning panel (322). By adjusting the X-direction fine-tuning micrometer head (321) knob, the fine-tuning platform (32) is driven to move horizontally in the X direction through the single guide rail structure to adjust the movement of the injection capillary in the X direction. The Y-direction fine-tuning micrometer head (323) is set on one side of the Y-direction fine-tuning panel (324), and the Y-direction fine-tuning micrometer head (323) knob is adjusted to drive the fine-tuning platform (32) to move horizontally in the Y direction through the single guide rail structure, thereby adjusting the movement of the injection capillary in the Y direction. The Z-direction fine-tuning micrometer head (325) is set on one side of the Z-direction fine-tuning panel (326), and the Z-direction fine-tuning micrometer head (325) knob is adjusted to drive the fine-tuning platform (32) to move horizontally in the Z direction through the single guide rail structure. , adjusting the movement of the injection capillary in the Z direction, the X-direction fine-tuning micrometer head (321), the Y-direction fine-tuning micrometer head (323), and the Z-direction fine-tuning micrometer head (325) are all electric micrometer heads, and the X-direction fine-tuning panel (322), the Y-direction fine-tuning panel (324), and the Z-direction fine-tuning panel (326) are internally provided with a driving device and are connected to the control system (5), and the clamping device (33) is fixed to one side of the Z-direction fine-tuning panel (326) by screw connection.

4. The automated fine-tuning microfluidic chip capillary coaxial arrangement device according to claim 1, characterized in that: The image system (4) includes an image system rear plate (41), an image system upper plate (42), a camera 1 (43), and a camera 2 (44). The image system rear plate (41) is vertically connected to the base (1), and a small hole is provided in the middle for fixing the camera 1 (43). The camera 1 (43) is used to observe the coaxial arrangement of the injection capillary and the collection capillary in the Z direction. The image system upper plate (42) is vertically connected to the image system rear plate (41), and a small hole is provided at one end for fixing the camera 2 (44). The camera 2 (44) is used to observe the coaxial arrangement of the injection capillary and the collection capillary in the X direction and the Y direction.

5. The automated fine-tuning microfluidic chip capillary coaxial arrangement device according to claim 1, characterized in that: The control system (5) includes a central console (51), a touch screen (52), an indicator light (53), a display screen (54), an operation keyboard (55) and a switch (56), wherein the touch screen (52) is arranged on the left side of the central console (51) and is used to turn on and off the system operation status; the indicator light (53) is arranged on the middle platform of the central console (51) and is used to send a signal prompt; the display screen (54) is arranged on the upper part of the central console (51) and is used to observe the three-dimensional coaxial arrangement position of the injection capillary and the collection capillary; the operation keyboard (55) is arranged on the middle platform of the central console (51) and is used to input control instructions; the switch (56) is arranged on the right side of the central console (51) and is used to turn on and off the central console (51); the control system (5) controls the displacement of the moving system (2) by driving the moving module, and controls the displacement of the fine adjustment system (3) by driving the fine adjustment module.

6. A method for preparing a microfluidic chip using the automated fine-tuning microfluidic chip capillary coaxial arrangement device according to any one of claims 1 to 5, characterized in that: The steps include: Step 1: Make the injection capillary and the collection capillary; Step 2: Place a glass slide on the device platform (23), fix it with a clip (24), insert the collecting capillary from one side of the connecting capillary, and fix both of them on the surface of the glass slide with an adhesive. The interface between the collecting capillary and the connecting capillary is connected and fixed by an external phase dispensing needle. The collecting capillary constitutes an external phase microchannel. The connecting capillary is a circular capillary. Place the obtained injection capillary at a fixed position of the clamping device (33), and turn the knob of the pressing device (331) to fix it. Step 3: Turn on the switch (56) of the central console (51), and input the program of the mobile system (2) on the central console (51) to set the control program; the operator turns on the system start button through the touch screen (52), and the mobile control module receives the movement instruction to drive the mobile system (2) to adjust the X-direction moving micrometer head (221), the Y-direction moving micrometer head (223), and the Z-direction moving micrometer head (225) to the initial position; then the fine-tuning control module receives the fine-tuning instruction to drive the fine-tuning system (3) to adjust the X-direction fine-tuning micrometer head (321), the Y-direction fine-tuning micrometer head (323), and the Z-direction fine-tuning micrometer head (325) to the initial position; Step 4: Camera 1 and Camera 2 capture the internal image of the microfluidic chip, obtain the position parameters (x, y, z) of the injection capillary and the collection capillary through the built-in displacement sensor, and transmit the position parameters to the control system (5). The image processing algorithm identifies the position parameters of the injection capillary and the collection capillary respectively and calculates the offset; Step 5: Based on the position parameter information provided by the image system (4), the control system (5) generates a fine-tuning instruction, which is sent to the fine-tuning system (3) through the fine-tuning control module. The X-direction fine-tuning micrometer head (321), the Y-direction fine-tuning micrometer head (323), and the Z-direction fine-tuning micrometer head (325) respectively execute the instruction to adjust the injection capillary in the X, Y, and Z directions, so that the injection capillary enters from the other side of the connecting capillary, so that the injection capillary and the collection capillary are both within the image capture range of the image system (4); Step 6: The image system (4) collects images of the adjusted positions of the injection capillary and the collection capillary in real time and performs position verification operations. If the injection capillary and the collection capillary are already coaxially arranged, the control system (5) sends a completion signal and saves the configuration data; if there is still a deviation, the control system (5) repeats the fine-tuning control steps until the injection capillary and the collection capillary reach a three-dimensional coaxial arrangement; Step 7: When the injection capillary and the collection capillary are in a three-dimensional coaxial arrangement, the interface between the injection capillary and the connecting capillary is fixed with an adhesive through an intermediate phase dispensing needle, and the injection capillary forms an inner phase microchannel.

7. The method according to claim 6, characterized in that The step 1 comprises: using a needle puller to process a circular glass capillary into two capillaries with a length of 5 cm and a tapered end, serving as an injection capillary and a collection capillary, wherein the inner diameter of the tapered tip of the injection capillary is 30 μm, and the inner diameter of the tapered tip of the collection capillary is 100 μm; cleaning and drying the obtained injection capillary and collection capillary to remove residual fine glass fragments; using octadecyltrichlorosilane to hydrophobically treat the injection capillary and collection capillary, and then cleaning them with ethanol and drying them for later use.

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