A modified collagen anti-wrinkle facial mask and its preparation method

Abstract

The present invention relates to the technical field of formulations for medical, dental or cosmetic use, and specifically discloses a modified collagen anti-wrinkle facial mask and a preparation method thereof. The facial mask comprises the following raw materials in parts by weight: 5-10 parts of modified fish collagen, 0.1-2 parts of sodium hyaluronate, 3-5 parts of propylene glycol, 2-5 parts of seabuckthorn oil, 2-5 parts of aloe extract, 2-5 parts of palmitoyl tripeptide-5, 2-5 parts of gentian root extract, 4-6 parts of a moisturizer, 0.1-2 parts of a thickener, 0.1-1 part of a surfactant, 0.01-0.1 part of a preservative, 0.2-0.6 part of an emulsifier, and 50-65 parts of water. The present invention also provides a preparation method thereof. Compared with the prior art, the facial mask prepared by the present invention has the advantages of good stability, obvious anti-aging effect, etc.

Description

Technical Field

[0001] The present invention relates to the technical field of formulations for medical, dental or cosmetic use, and particularly to a modified collagen anti-wrinkle facial mask and a preparation method thereof. Background Art

[0002] As time goes by, the skin will gradually show signs of aging, such as the appearance of wrinkles and the relaxation of facial contours. The occurrence of these aging phenomena is mainly the result of the interaction of various internal and external factors. Intrinsic aging is mainly due to the accumulation of advanced glycation end products (AGEs) over time, which leads to an increase in cross-linking between collagen. This change not only reduces the elasticity and breathability of the skin, but also weakens the exchange efficiency of nutrients and metabolic wastes, and makes the solubility of collagen decrease and it difficult to be decomposed. This ultimately causes the skin to lose its original elasticity, and the wrinkles become more obvious and difficult to recover. On the other hand, extrinsic aging is mainly caused by photoaging due to long-term exposure to sunlight. The radiation of ultraviolet rays will accelerate the aging process of the skin, making the skin lose firmness and increasing the number of wrinkles. These changes not only affect the appearance of the skin, but also make people look older than their actual age. Therefore, anti-aging and wrinkle-reducing skin care has become a long-term and continuous focus of care. By taking appropriate skin care measures, such as using skin care products with antioxidant and repair functions, and protecting the skin from excessive ultraviolet radiation, the aging process of the skin can be effectively delayed and the young state of the skin can be maintained.

[0003] CN201110079013.9 provides a collagen facial mask and a preparation method thereof. The method first prepares a stock solution by stirring and reacting a collagen aqueous solution with a mass fraction of (1-20)% and a modifier aqueous solution with a mass fraction of (0.1-2)% at a temperature of 20-60°C for 15-45 minutes. Then, a plasticizer, a functional additive and an anti-microbial growth agent are added to the stock solution, and the mixture is continuously stirred at a temperature of 20-60°C for 30-60 minutes to obtain a mixed solution. After that, the mixed solution is poured into a mold for shaping and dried at a temperature of 30-60°C for 3-10 hours to obtain the collagen facial mask. The collagen facial mask prepared by this invention is mainly composed of high-molecular substances extracted from natural animals and plants. The product is safe, effective, and has no toxic and side effects; it has good thermal stability, toughness, ductility and adhesiveness, and can fit well with the skin; with the assistance of functional additives, it can achieve dual moisturizing, softening horny skin, anti-wrinkle, whitening, repairing the skin and other effects; the mask waste can be degraded and does not cause environmental pollution; it can be widely applied in the field of daily cosmetics and has good market application prospects.

[0004] CN202311241251.4 relates to the field of natural active collagen modification technology, specifically to an acylated collagen, an acylated collagen preparation and its application. The acylated collagen refers to the product obtained by acid anhydride modification. The natural active collagen is dissolved in an acid solution, the pH is adjusted, a certain amount of acid anhydride is added, stirring is required during the reaction, and the pH is adjusted and a certain temperature is maintained to ensure a stable environment for the reaction. After the reaction, dialysis or ultrafiltration is carried out, and after completion, it is freeze-dried and stored to obtain the acid anhydride-modified acylated collagen. The acylated collagen preparation contains acylated collagen, and the use of the acylated collagen preparation in the preparation of drugs or cosmetics for anti-inflammatory, antioxidant, whitening, and promoting collagen secretion. The acylated collagen prepared by the invention has good water solubility, and retains the triple helix structure and biological activity of natural active collagen, has certain potential for promoting cell proliferation and cell migration, and can be used for cell culture.

[0005] Collagen is the most widely existing animal protein in nature. It has excellent adsorption and compatibility with the skin and has the function of blocking the generation of brown pigment. At the same time, the polypeptide chain of collagen hydrolysate contains hydrophilic groups such as amino and hydroxyl groups, which have a moisturizing effect on the human skin. In addition, collagen also has the functions of accelerating cell replication and proliferation, greatly increasing the metabolic rate of the stratum corneum, repairing damaged cells, reducing wrinkles and increasing elasticity, and efficiently locking water. However, collagen itself has the disadvantages of poor mechanical properties and thermal stability, which limit its application. Summary of the Invention

[0006] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to provide a modified collagen anti-wrinkle facial mask and its preparation method.

[0007] Collagen has unique triple - helix structural characteristics, and has good biocompatibility, biodegradability, and biological activity. Therefore, it has been widely used in the fields of food, medicine, tissue engineering, cosmetics, etc. However, some physicochemical properties of collagen are poor, such as thermal stability, mechanical strength, and enzyme resistance, which easily lead to unstable collagen structure or even denaturation. After fish collagen is hydrolyzed, it will produce collagen hydrolysates with similar amino acid compositions, which are extremely easy to be absorbed by the skin. The collagen hydrolysates that enter the skin epithelial cells through transdermal absorption will promote the activity of hyaluronic acid synthase. At the same time, the collagen hydrolysates absorbed through the skin are also beneficial to the collagen metabolism of the subcutaneous loose connective tissue, thereby improving the skin's ability to absorb moisture and enhancing the skin's elasticity. However, due to the defects existing in collagen itself, it will greatly affect its application and efficacy. Therefore, in the present invention, fish collagen is modified to improve the problem of its poor thermal stability, and to avoid inactivation and denaturation in the facial mask liquid, thus being unable to exert its efficacy. The inventor oxidizes biphenyl - 3,4 - diol under alkaline conditions and then covalently binds it to amino groups and sulfhydryl groups on the side chain of collagen, thereby effectively cross - linking. In addition, biphenyl - 3,4 - diol itself can also polymerize, and the polymer can also carry out covalent cross - linking, further enhancing the structural stability after cross - linking. The biphenyl structure in biphenyl - 3,4 - diol has strong rigidity and thermal stability, thus improving the stability of the modified fish collagen.

[0008] The seabuckthorn oil in the facial mask contains rich active substances such as flavonoids, vitamins, and anthocyanins. Therefore, it has various physiological activities such as antioxidant, moisturizing, and promoting skin metabolism. Aloe vera extract contains various functional ingredients such as aloe polysaccharides, proteins, organic acids, amino acids, and trace elements, which can play a role in skin rejuvenation, anti - aging, promote cholesterol synthesis, help change the composition of sebum, reduce oiliness, soften the skin, and at the same time can also antibacterial, anti - inflammatory, and moisturize. Palmitoyl tripeptide - 5 can target anti - aging, activate collagen fibers and elastic fibers in the dermis, enhance skin elasticity, tighten the skin, and fade wrinkles; Gentiana root extract can repair damaged skin, reduce irritation to the skin, and play an anti - itching and anti - allergic effect. Preparing a facial mask with these components together with the modified fish collagen can not only play a good moisturizing and hydrating effect, but also effectively reduce wrinkles and enhance skin elasticity.

[0009] To achieve the above object, the present invention provides a modified collagen anti-wrinkle facial mask, which comprises the following raw materials in parts by weight: 5-10 parts of modified fish collagen, 0.1-2 parts of sodium hyaluronate, 3-5 parts of propylene glycol, 2-5 parts of seabuckthorn oil, 2-5 parts of aloe extract, 2-5 parts of palmitoyl tripeptide-5, 2-5 parts of gentian root extract, 4-6 parts of humectant, 0.1-2 parts of thickener, 0.1-1 part of surfactant, 0.01-0.1 part of preservative, 0.2-0.6 part of emulsifier and 50-65 parts of water.

[0010] The preparation method of the modified fish collagen comprises the following steps:

[0011] X1. Add fish collagen into acetic acid solution, dialyze with water for 2-3 d, then carry out vacuum degassing and centrifugation at 0-5 °C. The lower-layer clear liquid is freeze-dried and then added into PBS buffer solution with pH = 7 to obtain mixture A;

[0012] X2. Add biphenyl-3,4-diol into 30% aqueous ethanol solution to obtain solution B. Mix mixture A and solution B, crosslink at 20-30 °C for 20-30 h, filter, wash with water, 70% aqueous ethanol solution and PBS buffer solution, and then wash with water and freeze-dry to obtain modified fish collagen.

[0013] Furthermore, the humectant is glycerol.

[0014] Furthermore, the thickener is hydroxyethyl cellulose.

[0015] Furthermore, the surfactant is Tween-40.

[0016] Furthermore, the preservative is p-hydroxyacetophenone.

[0017] Furthermore, the emulsifier is PEG-40 hydrogenated castor oil.

[0018] Preferably, the preparation method of the modified fish collagen comprises the following steps:

[0019] X1. Add fish collagen into 0.5 mol / L acetic acid solution, the concentration of fish collagen is 5-10 mg / mL, dialyze with water for 2-3 d, then carry out vacuum degassing and centrifugation at 0-5 °C. The lower-layer clear liquid is freeze-dried and then added into PBS buffer solution with pH = 7 to obtain mixture A;

[0020] X2. Add biphenyl - 3,4 - diol to 30% aqueous ethanol solution to obtain solution B, where the content of biphenyl - 3,4 - diol is 0.5 - 1 wt%. Mix mixture A and solution B in a volume ratio of 1:1 and cross - link at 20 - 30 °C for 20 - 30 h. After filtration, wash with water, 70% aqueous ethanol solution, and PBS buffer solution, and then wash with water and freeze - dry to obtain modified fish collagen.

[0021] A preparation method of a modified collagen anti - wrinkle facial mask, comprising the following steps:

[0022] S1. Add propylene glycol, humectant, thickener, and surfactant to water, heat and stir to mix evenly to obtain mixture A;

[0023] S2. After cooling mixture A, add sodium hyaluronate, stir and mix evenly, then add seabuckthorn oil and emulsifier, and continue to stir and mix evenly to obtain mixture B;

[0024] S3. After further cooling mixture B, add modified fish collagen, aloe extract, palmitoyl tripeptide - 5, gentian root extract, and preservative, mix evenly, filter through a 200 - mesh sieve to obtain the facial mask liquid, and pour the facial mask liquid into a packaging material containing a facial mask base cloth and seal to obtain the product.

[0025] Further, the heating temperature range in step S1 is 50 - 80 °C.

[0026] Further, the cooling temperature range in step S2 is 40 - 50 °C.

[0027] Further, the cooling temperature range in step S3 is 30 - 40 °C.

[0028] Advantages of the present invention:

[0029] 1. In the present invention, biphenyl - 3,4 - diol is oxidized under alkaline conditions and then covalently binds to amino groups and sulfhydryl groups on the side chains of collagen, thereby effectively cross - linking. In addition, biphenyl - 3,4 - diol itself can also polymerize, and the polymer can also carry out covalent cross - linking, further improving the structural stability after cross - linking. The biphenyl structure in biphenyl - 3,4 - diol has strong rigidity and thermal stability, thus improving the stability of the modified fish collagen.

[0030] 2. Preparing a facial mask by combining multiple active components with modified fish collagen can not only achieve good moisturizing and water - replenishing effects, but also effectively reduce wrinkles and enhance skin elasticity. The obtained facial mask has excellent heat resistance and cold resistance. Specific embodiments

[0031] Fish collagen, Mw = 1000 Da, Suzhou Zhuoxin Biotechnology.

[0032] Aloe vera extract, material-liquid ratio 10:1, Shaanxi Qingya Biotechnology Co., Ltd.

[0033] Gentiana root extract, material-liquid ratio 10:1, Shaanxi Haochen Biotechnology Co., Ltd.

[0034] PEG-40 hydrogenated castor oil, model: CO40, BASF

[0035] Control Example 1

[0036] A preparation method of a collagen anti-wrinkle mask, comprising the following steps:

[0037] S1. Add 4 g of propylene glycol, 6 g of glycerol, 1 g of hydroxyethyl cellulose, and 0.5 g of Tween-40 to 60 mL of water, heat to 60 °C and stir to obtain a homogeneous mixture A;

[0038] S2. After cooling the mixture A to 40 °C, add 1 g of sodium hyaluronate, stir and mix evenly, then add 4 g of seabuckthorn oil and 0.5 g of PEG-40 hydrogenated castor oil, and continue to stir and mix evenly to obtain a mixture B;

[0039] S3. After further cooling the mixture B to 30 °C, add 8 g of fish collagen, 5 g of aloe vera extract, 4 g of palmitoyl tripeptide-5, 5 g of gentiana root extract, and 0.05 g of p-hydroxyacetophenone, mix evenly, filter through a 200-mesh sieve to obtain the mask liquid, and pour the mask liquid into a packaging material containing a mask base cloth, and seal to obtain.

[0040] Example 1

[0041] A preparation method of a modified collagen anti-wrinkle mask, comprising the following steps:

[0042] S1. Add 4 g of propylene glycol, 6 g of glycerol, 1 g of hydroxyethyl cellulose, and 0.5 g of Tween-40 to 60 mL of water, heat to 60 °C and stir to obtain a homogeneous mixture A;

[0043] S2. After cooling the mixture A to 40 °C, add 1 g of sodium hyaluronate, stir and mix evenly, then add 4 g of seabuckthorn oil and 0.5 g of PEG-40 hydrogenated castor oil, and continue to stir and mix evenly to obtain a mixture B;

[0044] S3. After further cooling the mixture B to 30 °C, add 8 g of modified fish collagen, 5 g of aloe vera extract, 4 g of palmitoyl tripeptide-5, 5 g of gentiana root extract, and 0.05 g of p-hydroxyacetophenone, mix evenly, filter through a 200-mesh sieve to obtain the mask liquid, and pour the mask liquid into a packaging material containing a mask base cloth, and seal to obtain.

[0045] The preparation method of the modified fish collagen comprises the following steps:

[0046] X1. Add fish collagen into 0.5 mol / L acetic acid solution with the concentration of fish collagen being 8 mg / mL. After dialysis with water for 3 days, perform vacuum degassing and centrifugation at 4 °C. Add the lower-layer supernatant after freeze-drying into 50 mmol / L PBS buffer solution with pH = 7 to obtain mixture A;

[0047] X2. Add biphenyl-3,4-diol into 30% aqueous ethanol solution to obtain solution B with the content of biphenyl-3,4-diol being 1 wt%. Mix mixture A and solution B at a volume ratio of 1:1 and crosslink at 25 °C for 24 h. After filtration, wash with water, 70% aqueous ethanol solution, and PBS buffer solution, and then wash with water and perform freeze-drying to obtain modified fish collagen.

[0048] Example 2

[0049] A preparation method of a modified collagen anti-wrinkle facial mask, comprising the following steps:

[0050] S1. Add 4 g of propylene glycol, 6 g of glycerol, 1 g of hydroxyethyl cellulose, and 0.5 g of Tween-40 into 60 mL of water, heat to 60 °C and stir to mix evenly to obtain mixture A;

[0051] S2. After cooling mixture A to 40 °C, add 1 g of sodium hyaluronate, stir and mix evenly, then add 4 g of seabuckthorn oil and 0.5 g of PEG-40 hydrogenated castor oil, and continue to stir and mix evenly to obtain mixture B;

[0052] S3. After further cooling mixture B to 30 °C, add 8 g of modified fish collagen, 5 g of aloe extract, 4 g of palmitoyl tripeptide-5, 5 g of gentian root extract, and 0.05 g of p-hydroxyacetophenone, mix evenly, filter through a 200-mesh sieve to obtain the facial mask liquid, and pour the facial mask liquid into the packaging material containing the facial mask base cloth and seal to obtain the product.

[0053] The preparation method of the modified fish collagen comprises the following steps:

[0054] X1. Add fish collagen into 0.5 mol / L acetic acid solution with the concentration of fish collagen being 8 mg / mL. After dialysis with water for 3 days, perform vacuum degassing and centrifugation at 4 °C. Add the lower-layer supernatant after freeze-drying into 50 mmol / L PBS buffer solution with pH = 7 to obtain mixture A;

[0055] X2. Add 3-phenylphenol to an aqueous solution of 30% ethanol to obtain solution B with a 3-phenylphenol content of 1 wt%. Mix mixture A and solution B in a volume ratio of 1:1 and crosslink at 25 °C for 24 h. After filtration, wash with water, 70% aqueous ethanol solution, and PBS buffer solution, and then wash with water and freeze-dry to obtain modified fish collagen.

[0056] Example 3

[0057] A preparation method of a modified collagen anti-wrinkle facial mask, comprising the following steps:

[0058] S1. Add 4 g of propylene glycol, 6 g of glycerol, 1 g of hydroxyethyl cellulose, and 0.5 g of Tween-40 to 60 mL of water, heat to 60 °C and stir to obtain a homogeneous mixture A;

[0059] S2. After cooling mixture A to 40 °C, add 1 g of sodium hyaluronate, stir and mix evenly, then add 4 g of seabuckthorn oil and 0.5 g of PEG-40 hydrogenated castor oil, and continue to stir and mix evenly to obtain mixture B;

[0060] S3. After further cooling mixture B to 30 °C, add 8 g of modified fish collagen, 5 g of aloe extract, 4 g of palmitoyl tripeptide-5, 5 g of gentian root extract, and 0.05 g of p-hydroxyacetophenone, mix evenly, filter through a 200-mesh sieve to obtain the facial mask liquid, and pour the facial mask liquid into a packaging material containing a facial mask base cloth and seal to obtain.

[0061] The preparation method of the modified fish collagen comprises the following steps:

[0062] X1. Add fish collagen to a 0.5 mol / L acetic acid solution with a fish collagen concentration of 8 mg / mL, dialyze with water for 3 d, perform vacuum degassing and centrifugation at 4 °C, add the lower-layer supernatant after freeze-drying to a 50 mmol / L PBS buffer solution with pH = 7 to obtain mixture A;

[0063] X2. Add 3,3'-biphenol to an aqueous solution of 30% ethanol to obtain solution B with a 3,3'-biphenol content of 1 wt%. Mix mixture A and solution B in a volume ratio of 1:1 and crosslink at 25 °C for 24 h. After filtration, wash with water, 70% aqueous ethanol solution, and PBS buffer solution, and then wash with water and freeze-dry to obtain modified fish collagen.

[0064] Example 4

[0065] Same as Example 1, the only difference is that the modified fish collagen is 6 g.

[0066] Example 5

[0067] Same as Example 1, with the only difference being that the modified fish collagen is 10 g.

[0068] Test Example 1

[0069] A total of 210 female volunteer subjects with symptoms of skin relaxation and wrinkles were selected, with the oldest being 50 years old and the youngest being 35 years old. They were randomly divided into 6 groups of 30 people each. The wrinkle imprints of the test sites around the eyes of the subjects were obtained using silicone resin. The subjects used one facial mask every 2 days for four consecutive weeks, and the usage effect was evaluated after 28 days of use. The wrinkle images and imprints of the test sites around the eyes of the subjects were obtained using an image acquisition device and silicone resin, and the total wrinkle volume value was obtained through software analysis. Among them, the wrinkle volume value at the wrinkle target analysis position before using the sample was used as V1, and the wrinkle volume value at the wrinkle target analysis position 28 days after use was used as V2. The wrinkle change rate was calculated as = (V2 - V1) / V1 * 100%.

[0070] Table 1 Anti-wrinkle test of the anti-wrinkle facial mask with modified fish collagen

[0071] Experimental scheme Wrinkle change rate after 28 days of use / % Control Example 1 ﹣20.31 Example 1 ﹣28.65 Example 2 ﹣23.52 Example 3 ﹣23.63 Example 4 ﹣19.96 Example 5 ﹣28.01

[0072] After fish collagen is hydrolyzed, it will produce collagen hydrolysis products with similar amino acid compositions, which are extremely easy to be absorbed by the skin. The fish collagen hydrolysis products that enter the skin epithelial cells through transdermal absorption will promote the activity of hyaluronic acid synthase. At the same time, the collagen hydrolysis products absorbed through the skin are also beneficial to the collagen metabolism of the loose connective tissue under the skin, thereby improving the skin's ability to absorb moisture and enhancing the elasticity of the skin. The sea buckthorn oil in the facial mask contains rich active substances such as flavonoids, vitamins, and anthocyanins, so it has various physiological activities such as antioxidant, moisturizing, and promoting skin metabolism. Aloe vera extract contains various functional components such as aloe polysaccharides, proteins, organic acids, amino acids, and trace elements, which can play a role in activating the skin, anti-aging, promoting cholesterol synthesis, helping to change the composition of sebum, reducing oiliness, softening the skin, and at the same time can also antibacterial, anti-inflammatory, and moisturizing. Palmitoyl tripeptide-5 can target anti-aging, activate collagen fibers and elastic fibers in the dermis, enhance skin elasticity, tighten the skin, and fade wrinkles; Gentiana root extract can repair damaged skin, reduce irritation to the skin, and achieve the effect of relieving itching and preventing allergies. Preparing a facial mask with these components and modified fish collagen can not only achieve good moisturizing and hydrating effects, but also effectively reduce wrinkles and enhance skin elasticity. Therefore, the facial masks prepared by the present invention all have good anti-wrinkle effects. However, compared with Comparative Example 1 and Example 1, there are still certain differences in anti-wrinkle performance, which may be due to the poor stability of fish collagen itself, so it may denature or even inactivate during storage. In the examples, modifying fish collagen can well improve this problem, thereby ensuring the stability of the components. Compared with Examples 3-4, the difference in Example 1 is that it has a catechol structure, so it can also polymerize itself, and the polymer can also carry out covalent cross-linking, further improving the structural stability after cross-linking, so the efficacy is better. Compared with Example 1, the addition amounts of modified fish collagen in Examples 4-5 are different. Collagen is not soluble in water at room temperature, and the facial mask liquid itself is also a viscous liquid. If the addition amount is too much, it will not only cause a sticky feeling on the skin, but also easily cause stratification. Therefore, considering the efficacy performance, Example 1 is the best.

[0073] Test Example 2

[0074] Put the facial mask liquids in the control examples and examples into a constant temperature incubator at 50 °C for 24 h, and observe whether there are phenomena such as thinning, discoloration, stratification, and hardness change after returning to room temperature. Put the samples in a refrigerator at -10 °C for 24 h, and also observe whether there are phenomena such as thinning, discoloration, stratification, and hardness change after returning to room temperature. See Table 2 for details.

[0075] Table 2 Stability test of modified collagen anti-wrinkle facial mask liquid

[0076] Experimental scheme Thermal stability Low-temperature stability Control Example 1 Color change and delamination occurred No change occurred Example 1 No change occurred No change occurred Example 2 No change occurred No change occurred Example 3 No change occurred No change occurred Example 4 No change occurred No change occurred Example 5 No change occurred No change occurred

[0077] After fish collagen is hydrolyzed, collagen hydrolysates with similar amino acid compositions will be produced, which are extremely easy to be absorbed by the skin. The fish collagen hydrolysates that enter the skin epithelial cells through transdermal absorption will promote the activity of hyaluronic acid synthase. At the same time, the collagen hydrolysates absorbed through the skin are also beneficial to the collagen metabolism of the loose connective tissue under the skin, thereby improving the skin's ability to absorb moisture and enhancing the elasticity of the skin. However, collagen has low durability to high temperatures. Therefore, from the stability test, it can be seen that when the fish collagen is not modified, it shows intolerance at high temperatures, resulting in color change and stratification of the mask liquid. This will greatly affect the efficacy of the mask, while the mask liquid in the embodiment can maintain stability. This is because different components are used in the embodiment to crosslink and modify the collagen to increase the covalent crosslinking points in the collagen material, thereby restricting the movement of the collagen chain segments, resulting in a more stable collagen triple helix structure. Therefore, the heat resistance is improved. In addition, in Example 1, biphenyl-3,4-diol itself can also polymerize, and the polymer can also carry out covalent crosslinking, further enhancing the structural stability after crosslinking. The biphenyl structure in biphenyl-3,4-diol has strong rigidity and thermal stability, thus further improving the stability of the modified fish collagen.

[0078] The preferred specific embodiments of the present invention have been described in detail above. It should be understood that those of ordinary skill in the art can make many modifications and variations based on the concept of the present invention without creative work. Therefore, all technical solutions that can be obtained by those skilled in the art in the technical field of the present invention through logical analysis, reasoning, or limited experiments based on the concept of the present invention on the basis of the prior art should be within the protection scope determined by the claims.

Claims

1. A modified collagen anti-wrinkle facial mask, characterized in that: The invention comprises the following raw materials in parts by weight: 5-10 parts of modified fish collagen, 0.1-2 parts of sodium hyaluronate, 3-5 parts of propylene glycol, 2-5 parts of sea buckthorn oil, 2-5 parts of aloe extract, 2-5 parts of palmitoyl tripeptide-52, 2-5 parts of gentian root extract, 4-6 parts of moisturizer, 0.1-2 parts of thickener, 0.1-1 parts of surfactant, 0.01-0.1 parts of preservative, 0.2-0.6 parts of emulsifier and 50-65 parts of water; The preparation method of the modified fish collagen comprises the following steps: X1. Add fish collagen to 0.5 mol / L acetic acid solution, the concentration of fish collagen is 5-10 mg / mL, dialyze with water for 2-3 days, perform vacuum degassing and centrifugation at 0-5°C, freeze-dry the lower clear liquid, and add it to PBS buffer at pH=7 to obtain mixture A; X2. Add biphenyl-3,4-diol to 30% ethanol aqueous solution to obtain solution B, the content of biphenyl-3,4-diol is 0.5~1wt%, mix the mixture A and solution B in a volume ratio of 1:1, cross-link at 20~30°C for 20~30h, filter, wash with water, 70% ethanol aqueous solution, and PBS buffer, then wash with water and freeze-dry to obtain modified fish collagen.

2. The modified collagen anti-wrinkle facial mask according to claim 1, characterized in that: The humectant is glycerin.

3. The modified collagen anti-wrinkle facial mask according to claim 1, characterized in that: The thickener is hydroxyethyl cellulose.

4. The modified collagen anti-wrinkle facial mask according to claim 1, characterized in that: The surfactant is Tween-40.

5. The modified collagen anti-wrinkle facial mask according to claim 1, characterized in that: The preservative is p-hydroxyacetophenone.

6. The modified collagen anti-wrinkle facial mask according to claim 1, characterized in that: The emulsifier is PEG-40 hydrogenated castor oil.

7. A method for preparing the modified collagen anti-wrinkle facial mask according to any one of claims 1 to 6, characterized in that: The steps include: S1. Add propylene glycol, humectant, thickener and surfactant into water, heat and stir, and mix well to obtain a mixed solution A; S2, cooling the mixed solution A, adding sodium hyaluronate, stirring and mixing evenly, adding sea buckthorn oil and emulsifier, and continuing to stir and mix evenly to obtain a mixed solution B; S3. After the mixture B continues to cool down, add modified fish collagen, aloe extract, palmitoyl tripeptide-5, gentian root extract and preservatives, mix well and filter through a 200-mesh filter to obtain a facial mask liquid. Pour the facial mask liquid into the packaging material containing the facial mask base cloth and seal it.

8. The method for preparing the modified collagen anti-wrinkle facial mask according to claim 7, characterized in that: The heating temperature in step S1 is in the range of 50-80°C.

9. The method for preparing the modified collagen anti-wrinkle facial mask according to claim 7, characterized in that: The temperature range of cooling in step S2 is 40-50°C; the temperature range of cooling in step S3 is 30-40°C.

10. The method for preparing the modified collagen anti-wrinkle facial mask according to claim 7, characterized in that: The steps include: S1. Add 4 g of propylene glycol, 6 g of glycerol, 1 g of hydroxyethyl cellulose and 0.5 g of Tween-40 into 60 mL of water, heat to 60° C., stir and mix well to obtain a mixed solution A; S2, cooling the mixed solution A to 40° C., adding 1 g of sodium hyaluronate, stirring and mixing evenly, adding 4 g of sea buckthorn oil and 0.5 g of PEG-40 hydrogenated castor oil, and continuing to stir and mix evenly to obtain a mixed solution B; S3, after the mixed solution B is cooled to 30°C, 8g of modified fish collagen, 5g of aloe extract, 54g of palmitoyl tripeptide-1, 5g of gentian root extract and 0.05g of p-hydroxyacetophenone are added, mixed evenly and filtered through a 200-mesh filter to obtain a facial mask liquid, and the facial mask liquid is poured into a packaging material containing a facial mask base cloth and sealed; The preparation method of the modified fish collagen comprises the following steps: X1. Add fish collagen to 0.5 mol / L acetic acid solution to obtain a fish collagen concentration of 8 mg / mL. After dialyzing with water for 3 days, vacuum degassing and centrifugation are performed at 4°C. The lower clear liquid is freeze-dried and added to a 50 mmol / L PBS buffer solution at pH=7 to obtain a mixture A. X2. Add biphenyl-3,4-diol to 30% ethanol aqueous solution to obtain solution B, the content of biphenyl-3,4-diol is 1wt%, mix mixture A and solution B in a volume ratio of 1:1, cross-link at 25°C for 24h, filter, wash with water, 70% ethanol aqueous solution, and PBS buffer, then wash with water and freeze-dry to obtain modified fish collagen.