A plant extract separation and post-extraction detection device and detection method

Abstract

The present invention discloses a plant extract post-extraction detection device and detection method, including the following operation steps, first of all, the surface of the plant raw material needs to be cleaned to remove the dirt, dust and other impurities on the surface, and rinsed with tap water. During the cleaning process, care should be taken not to damage the structure of the sample or introduce new pollutants. In the present invention, when the length of the detection cup is short, the electric telescopic rod is controlled to work, and the electric telescopic rod moves the lifting plate upward during operation, and at the same time, the rotating block rotates in the rotating frame, so that the adjustment plate rotates synchronously. At this time, when the adjustment plate rotates, the slider in the top support slide slot slides accordingly, which can provide support for the adjustment plate when the angle is adjusted. When the angle of the adjustment plate is adjusted to a larger value, the inclination angle is larger, and the separation effect can be better when centrifugal separation is performed, so that the device can be adjusted for the detection cups used for different samples when in use, so that the device has a wider range of uses.

Description

Technical Field

[0001] The invention relates to the technical field of plant detection equipment, and in particular to a plant extract post-extraction detection device and a detection method. Background Art

[0002] The testing equipment for plant extracts after separation and extraction is mainly used to perform quality analysis and component identification on plant extracts that have been separated and extracted. These equipment can determine the purity of the extracts, their specific composition, and possible impurities.

[0003] When testing plant extracts, it is necessary to test the pesticide residues inside them to ensure that the pesticide residues inside the plants after cleaning cannot cause harm to the human body. The testing involves the operation of a series of devices, including centrifuges for centrifugal separation. When the existing centrifugal force is used for centrifugal separation of plant extracts, due to the different sizes of samples, the lengths of the test cups used are different. Shorter test cups can generally be separated smoothly, while longer test cups cannot be accommodated and rotated during testing due to their limited length. Improvements are needed to address this problem. Summary of the invention

[0004] The object of the present invention is to provide a plant extract separation and post-extraction detection device and detection method to solve the problems raised in the above background technology.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions: a method for detecting plant extracts after separation and extraction, comprising the following steps:

[0006] Sample preparation

[0007] First, the surface of the plant material needs to be cleaned to remove dirt, dust and other impurities on the surface. This can be done by rinsing with tap water, gently brushing or ultrasonic cleaning. During the cleaning process, care should be taken not to damage the structure of the sample or introduce new contaminants.

[0008] Sample crushing

[0009] In order to increase the contact area between the sample and the extraction solvent and improve the extraction efficiency, it is usually necessary to crush the cleaned sample. A crusher can be used for crushing. The crushed sample should maintain uniformity for subsequent extraction and detection.

[0010] Sample preparation

[0011] Transfer the crushed plant sample to a beaker, and then add a certain volume of acetonitrile. The ratio of acetonitrile to sample is 10 mL acetonitrile corresponding to 10 g sample. Mix the acetonitrile and plant sample thoroughly to promote the dissolution of pesticide residues from plant tissues into acetonitrile.

[0012] Centrifugal separation

[0013] After the extraction is completed, the sample solution can be centrifuged using a centrifugal separation device to remove solid particles and impurities therein.

[0014] Purification

[0015] The clear liquid on the top of the extract was taken out, and then the clear liquid was purified and analyzed by solid phase extraction.

[0016] analyze

[0017] The purified clear solution was transferred to a chromatographic analyzer for analysis of pesticide residues.

[0018] Report Writing

[0019] Organize the test results into a report, including sample information, chromatographic conditions, test results, conclusions, etc. The report should describe in detail the operating procedures and results of steps such as sample pretreatment, chromatographic condition setting, sample injection, chromatographic analysis, and data processing.

[0020] A plant extract separation and post-extraction detection device comprises a crusher, a centrifugal separation device and a chromatographic analysis instrument arranged in sequence, wherein the crusher and the chromatographic analyzer are existing equipment and are not described in detail here, wherein the centrifugal separation device comprises a supporting base, a driving mechanism, an adaptive adjustment mechanism and a supporting mechanism, wherein a driving separation box is fixedly connected to the top of the supporting base, and a protective cover is hinged on the top hinge of the back of the driving separation box.

[0021] According to the above technical solution, the driving mechanism includes a driving motor, a rotating shaft, an electric telescopic rod and a lifting plate. The driving motor is fixedly connected to the middle of the bottom of the driving separation box. The driving motor has an output shaft. The rotating shaft is fixedly connected to the output shaft of the driving motor. The electric telescopic rod is fixedly connected to the top of the rotating shaft. The lifting plate is fixedly connected to the bottom of the electric telescopic rod.

[0022] According to the above technical solution, the adaptive adjustment mechanism includes a rotating frame 1, a rotating block, an adjustment plate, a gear shaft 1, a gear shaft 2, an angle adjustment disk, a positioning magnetic block and a fixed clamp. The rotating frame 1 is fixedly connected to the right side of the lifting disk, the rotating block is rotatably connected to the middle part of the rotating frame 1, the adjustment plate is fixedly connected to the right side of the rotating block, the gear shaft 2 is rotatably connected to the top inside the adjustment plate, the gear shaft 1 is rotatably connected to the top inside the adjustment plate and is located below the gear shaft 2, the angle adjustment disk is fixedly connected to the front face of the gear shaft 1, the fixed clamp is fixedly connected to the front and rear ends of the right side of the gear shaft 2, the positioning magnetic block is magnetically attracted to the top of the front face of the adjustment plate and is cross-arranged with the angle adjustment disk.

[0023] According to the above technical solution, the supporting mechanism includes a rotating frame 2, a supporting connecting plate, a rotating frame 3, a sliding block and a supporting sliding groove. The rotating frame 2 is fixedly connected to the right side of the rotating shaft, the supporting connecting plate is rotatably connected to the inner middle part of the rotating frame 2, the rotating frame 3 is rotatably connected to the side of the supporting connecting plate away from the rotating frame 2, the sliding block is fixedly connected to the right side of the rotating frame 3, and the supporting sliding groove is fixedly connected to the left side of the adjustment plate.

[0024] According to the above technical solution, the output shaft of the driving motor passes through the bottom of the driving separation box and extends into the driving separation box to be fixedly connected to the bottom of the rotating shaft. A sliding groove is provided in the lifting plate, and the lifting plate is slidingly connected to the rotating shaft through the sliding groove, so that the lifting plate can slide on the rotating shaft housing.

[0025] According to the above technical solution, the slider is located in the supporting slide groove, and the slider is slidably connected to the supporting slide groove, so that the slider can slide in the supporting slide groove, and the gear shaft one is meshedly connected with the outer side of the gear shaft two.

[0026] Compared with the prior art, the beneficial effects achieved by the present invention are as follows: in the present invention, when a relatively long detection cup needs to be centrifuged, the positioning magnetic block is pulled out so that the angle adjustment disk can be rotated, and then the angle adjustment disk is rotated to drive the gear shaft 1 to rotate, so that the gear shaft 1 drives the gear shaft 2 meshing at the top to rotate, and then the fixed clamp on the gear shaft 2 is adjusted in angle, and the angle of the detection cup in the fixed clamp can be adjusted, and then the driving motor is started, and the driving motor drives the rotating shaft to rotate when working, and then the detection cup in the fixed clamp is centrifuged;

[0027] When the length of the detection cup is shorter, the electric telescopic rod is controlled to work. The electric telescopic rod moves the lifting plate upward during operation. At the same time, the rotating block rotates in the rotating frame one, so that the adjustment plate rotates synchronously. At this time, when the adjustment plate rotates, the slider in the top supporting slide slot slides accordingly, so that the supporting connecting plate in the rotating frame three on the slider rotates. At this time, the other side of the supporting connecting plate rotates in the rotating frame two, which can provide support for the adjustment plate when the angle is adjusted. When the angle of the adjustment plate is adjusted to a large extent, the inclination angle is made larger, and the separation effect can be better during centrifugal separation. When the device is in use, it can be adjusted for detection cups used for different samples, making the device more widely used. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0029] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0030] Figure 2 It is a schematic diagram of the interior of the drive separation box of the present invention;

[0031] Figure 3 It is a schematic diagram of explosion at the lifting plate of the present invention;

[0032] Figure 4 It is a schematic diagram of a gear shaft of the present invention;

[0033] Figure 5 This is an exploded schematic diagram of the positioning magnetic block of the present invention;

[0034] Figure 6 It is a schematic diagram of three parts of the rotating frame of the present invention;

[0035] Figure 7 It is a schematic diagram of the supporting chute of the present invention.

[0036] In the figure: 1. support base; 2. drive separation box; 3. protective cover; 4. drive mechanism; 401. drive motor; 402. rotating shaft; 403. electric telescopic rod; 404. lifting plate; 5. adaptive adjustment mechanism; 501. rotating frame one; 502. rotating block; 503. adjustment plate; 504. gear shaft one; 505. gear shaft two; 506. angle adjustment plate; 507. positioning magnetic block; 508. fixing clamp; 6. support mechanism; 601. rotating frame two; 602. support connecting plate; 603. rotating frame three; 604. slider; 605. support slide groove. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] See also Figure 1-3 The present invention provides a technical solution: a method for detecting plant extracts after separation and extraction, comprising the following steps:

[0039] Sample preparation

[0040] First, the surface of the plant material needs to be cleaned to remove dirt, dust and other impurities on the surface. Then rinse with tap water, gently brush or ultrasonic cleaning, etc. During the cleaning process, care should be taken not to damage the structure of the sample or introduce new contaminants.

[0041] Sample crushing

[0042] In order to increase the contact area between the sample and the extraction solvent and improve the extraction efficiency, it is usually necessary to crush the cleaned sample using a crusher. The crushed sample should maintain uniformity for subsequent extraction and testing.

[0043] Sample preparation

[0044] Transfer the crushed plant sample to a beaker, and then add a certain volume of acetonitrile. The ratio of acetonitrile to sample is 10 mL acetonitrile corresponding to 10 g sample. Mix the acetonitrile and plant sample thoroughly to promote the dissolution of pesticide residues from plant tissues into acetonitrile.

[0045] Centrifugal separation

[0046] After the extraction is completed, the sample solution can be centrifuged using a centrifugal separation device to remove solid particles and impurities therein.

[0047] Purification

[0048] The clear liquid on the top of the extract was taken out, and then the clear liquid was purified and analyzed by solid phase extraction.

[0049] analyze

[0050] The purified supernatant was transferred to a chromatographic analyzer for analysis of pesticide residues.

[0051] Report Writing

[0052] Organize the test results into a report, including sample information, chromatographic conditions, test results, conclusions, etc. The report should describe in detail the operating procedures and results of steps such as sample pretreatment, chromatographic condition setting, sample injection, chromatographic analysis, and data processing.

[0053] A plant extract separation and post-extraction detection device comprises a crusher, a centrifugal separation device and a chromatographic analyzer arranged in sequence, wherein the crusher and the chromatographic analyzer are existing equipment and are not described in detail here; the centrifugal separation device comprises a support base 1, a driving mechanism 4, an adaptive adjustment mechanism 5 and a supporting mechanism 6; a driving separation box 2 is fixedly connected to the top of the support base 1, and a protective cover 3 is hinged on the top of the back of the driving separation box 2.

[0054] The driving mechanism 4 includes a driving motor 401, a rotating shaft 402, an electric telescopic rod 403 and a lifting plate 404. The driving motor 401 is fixedly connected to the middle of the bottom of the driving separation box 2. The driving motor 401 has an output shaft. The rotating shaft 402 is fixedly connected to the output shaft of the driving motor 401. The electric telescopic rod 403 is fixedly connected to the top of the rotating shaft 402. The lifting plate 404 is fixedly connected to the bottom of the electric telescopic rod 403.

[0055] The output shaft of the driving motor 401 passes through the bottom of the driving separation box 2 and extends into the driving separation box 2 to be fixedly connected to the bottom of the rotating shaft 402. A sliding groove is provided in the lifting plate 404. The lifting plate 404 is slidingly connected to the rotating shaft 402 through the sliding groove, so that the lifting plate 404 can slide on the shell of the rotating shaft 402.

[0056] See also Figure 4-7 The present invention provides a technical solution: the adaptive adjustment mechanism 5 includes a rotating frame 501, a rotating block 502, an adjustment plate 503, a gear shaft 1 504, a gear shaft 2 505, an angle adjustment disk 506, a positioning magnet 507 and a fixing clamp 508, the rotating frame 501 is fixedly connected to the outer side of the lifting disk 404, the rotating block 502 is rotatably connected to the middle part of the rotating frame 501, the adjustment plate 503 is fixedly connected to the right side of the rotating block 502, the gear shaft 2 505 is rotatably connected to the top inside the adjustment plate 503, the gear shaft 1 504 is rotatably connected to the top inside the adjustment plate 503 and is located below the gear shaft 2 505, the angle adjustment disk 506 is fixedly connected to the front side of the gear shaft 1 504, the fixing clamp 508 is fixedly connected to the front and rear ends of the right side of the gear shaft 2 505, the positioning magnet 507 is magnetically attracted to the top of the front side of the adjustment plate 503, and is cross-arranged with the angle adjustment disk 506.

[0057] The supporting mechanism 6 includes a rotating frame 2 601, a supporting connecting plate 602, a rotating frame 3 603, a slider 604 and a supporting slide groove 605. The rotating frame 2 601 is fixedly connected to the outer side of the rotating shaft 402, the supporting connecting plate 602 is rotatably connected to the middle part of the rotating frame 2 601, the rotating frame 3 603 is rotatably connected to the side of the supporting connecting plate 602 away from the rotating frame 2 601, the slider 604 is fixedly connected to the outer side of the rotating frame 3 603, and the supporting slide groove 605 is fixedly connected to the outer side of the adjustment plate 503.

[0058] The slider 604 is located in the supporting slide groove 605 , and the slider 604 is slidably connected to the supporting slide groove 605 , so that the slider 604 can slide in the supporting slide groove 605 .

[0059] The gear shaft 1 504 is meshedly connected with the outer side of the gear shaft 2 505 .

[0060] When it is necessary to centrifuge a longer detection cup, the positioning magnetic block 507 is unplugged to allow the angle adjustment disk 506 to rotate, and then the angle adjustment disk 506 is rotated to drive the gear shaft 1 504 to rotate, so that the gear shaft 1 504 drives the top meshing gear shaft 2 505 to rotate, and then the fixed clamp 508 on the gear shaft 2 505 is adjusted in angle, and the angle of the detection cup in the fixed clamp 508 can be adjusted, and then the drive motor 401 is started. When working, the drive motor 401 drives the rotating shaft 402 to rotate, and then the detection cup in the fixed clamp 508 is centrifuged.

[0061] When the length of the detection cup is shorter, the electric telescopic rod 403 is controlled to work. The electric telescopic rod 403 moves the lifting plate 404 upward during operation, and the rotating block 502 rotates in the rotating frame 1 501 at the same time, so that the adjusting plate 503 rotates synchronously. At this time, when the adjusting plate 503 rotates, the slider 604 in the top supporting slide groove 605 slides accordingly, so that the supporting connecting plate 602 in the rotating frame 3 603 on the slider 604 rotates. At this time, the other side of the supporting connecting plate 602 rotates in the rotating frame 2 601, which can provide support for the adjusting plate 503 when the angle is adjusted. When the angle of the adjusting plate 503 is adjusted to a large extent, the inclination angle is made larger, so that the separation effect can be better during centrifugal separation.

[0062] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0063] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A plant extract separation and post-extraction detection device, comprising a crusher, a centrifugal separation device and a chromatographic analysis instrument arranged in sequence, characterized in that: The centrifugal separation device comprises a support base (1), a driving mechanism (4), an adaptive adjustment mechanism (5) and a support mechanism (6); the top of the support base (1) is fixedly connected to a driving separation box (2); the top of the back of the driving separation box (2) is hingedly connected to a protective cover (3); The driving mechanism (4) comprises a driving motor (401), a rotating shaft (402), an electric telescopic rod (403) and a lifting plate (404); the driving motor (401) is fixedly connected to the middle of the bottom of the driving separation box (2); the driving motor (401) has an output shaft; the rotating shaft (402) is fixedly connected to the output shaft of the driving motor (401); the electric telescopic rod (403) is fixedly connected to the top of the rotating shaft (402); and the lifting plate (404) is fixedly connected to the bottom of the electric telescopic rod (403); The adaptive adjustment mechanism (5) comprises a rotating frame (501), a rotating block (502), an adjustment plate (503), a gear shaft (504), a gear shaft (505), an angle adjustment plate (506), a positioning magnetic block (507) and a fixing clamp (508), wherein the rotating frame (501) is fixedly connected to the right side of the lifting plate (404), the rotating block (502) is rotatably connected to the middle part of the rotating frame (501), the adjustment plate (503) is fixedly connected to the right side of the rotating block (502), and the gear shaft (505) is rotatably connected to the lifting plate (404). Connected to the top of the adjustment plate (503), the gear shaft one (504) is rotatably connected to the top of the adjustment plate (503) and is located below the gear shaft two (505), the angle adjustment disk (506) is fixedly connected to the front of the gear shaft one (504), the fixing clamp (508) is fixedly connected to the front and rear ends of the right side of the gear shaft two (505), the positioning magnetic block (507) is magnetically attracted to the top of the front of the adjustment plate (503) and is arranged crosswise with the angle adjustment disk (506), and the gear shaft one (504) is meshedly connected with the outer side of the gear shaft two (505); The support mechanism (6) comprises a rotating frame 2 (601), a supporting connecting plate (602), a rotating frame 3 (603), a sliding block (604) and a supporting sliding groove (605), wherein the rotating frame 2 (601) is fixedly connected to the right side of the rotating shaft (402), the supporting connecting plate (602) is rotatably connected to the middle part of the rotating frame 2 (601), the rotating frame 3 (603) is rotatably connected to the side of the supporting connecting plate (602) away from the rotating frame 2 (601), the sliding block (604) is fixedly connected to the right side of the rotating frame 3 (603), and the supporting sliding groove (605) is fixedly connected to the left side of the adjusting plate (503); The output shaft of the driving motor (401) passes through the bottom of the driving separation box (2) and extends into the driving separation box (2) to be fixedly connected to the bottom of the rotating shaft (402); a sliding groove is provided in the lifting plate (404); and the lifting plate (404) is slidably connected to the rotating shaft (402) via the sliding groove; The sliding block (604) is located in the supporting sliding groove (605), and the sliding block (604) is slidably connected to the supporting sliding groove (605).

2. The detection method of a plant extract separation and extraction detection device according to claim 1, characterized in that: The steps include: Sample preparation First, the surface of the plant material is cleaned to remove dirt and dust on the surface, and then rinsed with tap water to obtain a sample; Sample crushing The cleaned samples are crushed using a crusher; Sample preparation Transfer the crushed sample into a beaker, then add acetonitrile. The ratio of acetonitrile to sample is 10 mL acetonitrile to 10 g sample. Mix the acetonitrile and plant sample thoroughly to promote the dissolution of pesticide residues from plant tissues into acetonitrile to obtain a sample solution. Centrifugal separation The sample solution is centrifuged using a centrifugal separation device to remove solid particles and impurities therein to obtain an extract; Purification The clear liquid on the top of the extract is taken out, and then the clear liquid is purified and analyzed by solid phase extraction; analyze The purified clear liquid is transferred to a chromatographic analysis instrument for analysis of pesticide residues; Report Writing The test results are organized into a report that includes sample information, chromatographic conditions, test results, and conclusions.

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