Laminate and method for producing the same

Abstract

A method for producing a laminate, comprising: a first application step of applying a first adhesive to a first surface of a first member made of silicone rubber, the first adhesive including a silane coupling agent having an epoxy group; a second application step of applying a second adhesive to a second surface of a second member made of polystyrene, the second adhesive including a silane coupling agent having an amino group; and a joining step of joining the first surface of the first member and the second surface of the second member to each other with the first adhesive and the second adhesive.

Description

Technical Field

[0001] This invention relates to a technique for joining multiple components together. Background Technology

[0002] Traditionally, a technique has been proposed for bonding multiple components made of different materials using silane coupling agents. For example, Patent Document 1 discloses a technique in which a sheet made of silicone rubber and a sheet made of resin material or metal material are bonded together by a single type of silane coupling agent.

[0003] Related technical documents

[0004] Patent documents

[0005] Patent Document 1: Japanese Patent Application Early Publication No. 2014-62224 Summary of the Invention

[0006] The problem to be solved by the present invention

[0007] However, when using a single type of silane coupling agent, it is difficult to adequately guarantee the bonding durability between components, and problems often arise (e.g., one component detaches from the other), especially in high humidity environments. In view of the above, one aspect of this disclosure aims to adequately ensure the bonding durability of the first and second components.

[0008] Problem Solving

[0009] According to one aspect of the invention, a laminate includes: a first component made of silicone rubber; a second component made of polystyrene; and an adhesive layer that bonds a first surface of the first component and a second surface of the second component to each other, wherein the adhesive layer includes: a first layer disposed on the first surface, the first layer being made of a silane coupling agent having epoxy groups; and a second layer disposed on the second surface, the second layer being made of a silane coupling agent having amino groups.

[0010] According to another aspect of the invention, a method for producing a laminate includes: a first application step: applying a first adhesive to a first surface of a first component, the first component being made of silicone rubber, the first adhesive comprising a silane coupling agent having epoxy groups; a second application step: applying a second adhesive to a second surface of a second component, the second component being made of polystyrene, the second adhesive comprising a silane coupling agent having amino groups; and a bonding step: bonding the first surface of the first component and the second surface of the second component together with the first adhesive and the second adhesive. The order of the first application step and the second application step can be freely chosen. For example, the above aspects include a mode in which the second application step is performed after the first application step, a mode in which the first application step is performed after the second application step, or a mode in which the first application step and the second application step are performed in parallel, either partially or entirely.

[0011] Invention Effects

[0012] According to the present invention, the durability of the engagement between the first component and the second component can be fully ensured. Attached Figure Description

[0013] Figure 1 This is a cross-section of the laminate according to the embodiment.

[0014] Figure 2 It is an illustrative diagram showing the mutual engagement of the first and second components.

[0015] Figure 3 It is a flowchart showing the production process of laminates. Detailed Implementation

[0016] A: Laminate

[0017] Figure 1 This is a cross-section of the laminate 100 according to an embodiment of the present invention. The laminate 100 according to an embodiment of the present invention is, for example, a well plate for culturing various types of cells (more specifically, spherical cells), and includes a first component 10, a second component 20, and an adhesive layer 30.

[0018] The first component 10 is a molded body in the shape of a thin sheet made of silicone rubber. In other words, the first component 10 is a component comprising silicone rubber material. Silicone rubber is, for example, polydimethylsiloxane (PDMS). The first component 10 includes a first surface 11. The first surface 11 is provided with a plurality of small grooves (not shown). For example, each of these grooves is a hemispherical groove with a diameter of about 1 mm.

[0019] The second component 20 is a molded body in the shape of a sheet made of polystyrene (PS). In other words, the second component 20 is a component comprising polystyrene material. The first component 10 is made of a resin material, while the second component 20 is made of a different resin material. The external dimensions of the first component 10 and the second component 20 are substantially the same. The second component 20 includes a second surface 21. The first surface 11 of the first component 10 and the second surface 21 of the second component 20 face each other. The second component 20 has a plurality of through holes 22. Each of the plurality of through holes 22 is a circular opening through the second component 20. The plurality of through holes 22 are arranged in a matrix. The diameter of each of the plurality of through holes 22 is significantly larger than the diameter of each groove on the first surface 11. Each of the plurality of through holes 22 has an inner surface that defines a space for accommodating cells to be cultured, the space being further defined by the first surface 11 of the first component 10, which forms the bottom plane of the space.

[0020] An adhesive layer 30 is located between the first component 10 and the second component 20. The adhesive layer 30 is a bonding material used to bond the first surface 11 and the second surface 21 together. The adhesive layer 30 is constructed of a laminate consisting of a first layer 31 and a second layer 32. The first layer 31 is made of a first adhesive applied to the first surface 11. The second layer 32 is made of a second adhesive applied to the second surface 21. The first component 10 and the second component 20 are bonded together by bonding the first adhesive and the second adhesive together. The first adhesive is made of one material, while the second adhesive is made of another material. Specifically, the first adhesive includes a silane coupling agent with functional groups, while the second adhesive includes another silane coupling agent with functional groups, and the functional groups of the silane coupling agent included in the first adhesive are different from those of the silane coupling agent included in the second adhesive.

[0021] The first adhesive comprises a silane coupling agent (hereinafter referred to as "epoxysilane coupling agent") having an epoxy group as a functional group. Examples of epoxysilane coupling agents include 3-glycidoxypropyltrimethoxysilane (having a glycidyl group, including an epoxy group) and 2-(3,4-epoxycyclohexyl)ethyltrimethoxysilane (having an epoxy group). As epoxysilane coupling agents having a glycidyl group, 3-glycidoxypropylmethyldimethoxysilane, 3-glycidoxypropylmethyldiethoxysilane, or 3-glycidoxypropyltriethoxysilane may be used.

[0022] The second adhesive comprises a silane coupling agent having an amino group as a functional group (hereinafter referred to as "aminosilane coupling agent"). Examples of aminosilane coupling agents include 3-aminopropyltrimethoxysilane. For example, 3-aminopropyltriethoxysilane, N-2-(aminoethyl)-3-aminopropylmethyldimethoxysilane, or N-2-(aminoethyl)-3-aminopropyltrimethoxysilane can be used as aminosilane coupling agents.

[0023] like Figure 2 As shown, the first component 10 and the second component 20 are bonded together by chemical bonds between the epoxy groups of the epoxy silane coupling agent (first adhesive) included in the first layer 31 of the adhesive layer 30 and the amino groups of the amino silane coupling agent (second adhesive) included in the second layer 32. Specifically, a chemical bond is formed by the reaction of two epoxy groups of the first layer 31 with one amino group of the second layer 32.

[0024] As described above, in this embodiment, the first component 10 and the second component 20 are bonded to each other by an adhesive layer 30, which includes a first layer 31 and a second layer 32. The first layer 31 is formed on a first surface 11 of the first component 10, which is made of silicone rubber. The second layer 32 is formed on a second surface 21 of the second component 20, which is made of polystyrene. The first layer 31 is made of a silane coupling agent having epoxy groups, while the second layer 32 is made of a silane coupling agent having amino groups. Therefore, compared to a configuration where the first component 10 and the second component 20 are bonded by a single type of silane coupling agent, the bond between the first component 10 and the second component 20 maintains higher durability. Furthermore, the adhesive strength of the adhesive layer 30 is improved; therefore, moisture penetration can be reduced, thereby improving water resistance.

[0025] B: Method for producing laminate 100

[0026] Figure 3 This is a flowchart showing the production process of laminate 100. For example... Figure 3 As shown, the production process of the laminate 100 includes: a first step Sa related to the first component 10 and the first layer 31, a second step Sb related to the second component 20 and the second layer 32, and a joining step Sc that joins the first component 10 and the second component 20 together.

[0027] B1: Step 1 Sa

[0028] The first step Sa includes a first activation step Sa1, a first preparation step Sa2, and a first application step Sa3. In Figure 3 While the example conveniently shows the case where the first activation step Sa1 is executed followed by the first preparation step Sa2, the timing relationship between the first activation step Sa1 and the first preparation step Sa2 can be freely chosen. In other words, the first activation step Sa1 can be executed after the first preparation step Sa2, or alternatively, the first activation step Sa1 and the first preparation step Sa2 can be executed in parallel.

[0029] In the first activation step Sa1, a surface treatment is performed in which the first surface 11 of the first component 10 is activated by a light beam. Specifically, the surface treatment in the first activation step Sa1 is a process of projecting an excimer laser beam (vacuum ultraviolet: VUV) onto the first surface 11. For example, specifically, the excimer beam is projected onto the first surface 11 within 15 seconds.

[0030] In the first preparation step Sa2, a first adhesive (including an epoxy silane coupling agent) is prepared. Specifically, the first adhesive is produced by mixing the epoxy silane coupling agent and a solvent. The solvent is, for example, a mixture of ethanol and water in a 9:1 ratio, or a mixture of isopropanol (IPA) and water in a 9:1 ratio. However, the type of solvent is not limited to the examples above. In the first preparation step Sa2, the first adhesive is prepared by mixing the epoxy silane coupling agent into the solvent, and the concentration of the epoxy silane coupling agent in the first adhesive is 1% by weight (wt%) or more and 10% by weight or less.

[0031] After performing the first activation step Sa1 and the first preparation step Sa2, the first application step Sa3 is performed. In the first application step Sa3, the first adhesive is applied to the first surface 11 of the first component 10. For example, the first adhesive can be applied using a brush, sprayer, or roller. The first surface 11 of the first component 10 is immersed in the first adhesive, and the first adhesive can be applied to the first surface 11. A specific example of the first step Sa is as described above.

[0032] B2: Step Two Sb

[0033] The second step Sb includes a second activation step Sb1, a second preparation step Sb2, and a second application step Sb3. Figure 3 Although the example conveniently shows the second preparation step Sb2 being executed after the second activation step Sb1, the timing relationship between the second activation step Sb1 and the second preparation step Sb2 can be freely chosen. In other words, the second activation step Sb1 can be executed after the second preparation step Sb2, or alternatively, the second activation step Sb1 and the second preparation step Sb2 can be executed in parallel.

[0034] In the second activation step Sb1, a surface treatment is performed in which the second surface 21 of the second component 20 is activated by a light beam. Specifically, similar to the first activation step Sa1, the surface treatment in the second activation step Sb1 is the process of projecting an excimer beam onto the second surface 21. For example, specifically, the excimer beam is projected onto the second surface 21 for 30 seconds. In other words, the time for the excimer beam to be projected onto the second surface 21 in the second activation step Sb1 is longer than the time for the excimer beam to be projected onto the first surface 11 in the first activation step Sa1. However, the specific time periods of the first activation step Sa1, the specific time periods of the second activation step Sb1, and the difference between the time periods of the first activation step Sa1 and the second activation step Sb1 are not limited to the examples described above.

[0035] In the second preparation step Sb2, a second adhesive comprising an aminosilane coupling agent is prepared. Specifically, the second adhesive is prepared by mixing the aminosilane coupling agent and a solvent. The solvent is, for example, a mixture of ethanol and water in a 9:1 ratio, or a mixture of isopropanol (IPA) and water in a 9:1 ratio. However, the type of solvent is not limited to the examples above. In the second preparation step Sb2, the second adhesive is prepared by mixing the aminosilane coupling agent into the solvent, and the concentration of the aminosilane coupling agent in the second adhesive is 1% by weight or more and 10% by weight or less.

[0036] After performing the second activation step Sb1 and the second preparation step Sb2, the second application step Sb3 is performed. In the second application step Sb3, the second adhesive is applied to the second surface 21 of the second component 20. For example, similar to the first coating step Sa3 described above, a brush, sprayer, or roller can be used to apply the second adhesive. The second adhesive can be applied to the second surface 21 by immersing it in the second adhesive. A specific example of the second step Sb is as described above.

[0037] B3: Joining Steps Sc

[0038] The joining step Sc is performed after the first step Sa and the second step Sb. In the joining step Sc, the first surface 11 of the first component 10 and the second surface 21 of the second component 20 are joined together by a first adhesive and a second adhesive. The joining step Sc begins approximately 5 minutes after the completion of the first step Sa and the second step Sb. The joining step Sc includes a placement step Sc1 and a pressure joining step Sc2.

[0039] In placement step Sc1, the first component 10 and the second component 20 are positioned such that the first adhesive on the first surface 11 and the second adhesive on the second surface 21 come into contact with each other. In placement step Sc1, excess solution is removed by blowing air onto the first adhesive on the first surface 11 and the second adhesive on the second surface 21. Furthermore, in placement step Sc1, by removing foam between the first adhesive and the second adhesive, the first adhesive and the second adhesive are fully bonded without any gaps.

[0040] In the pressure bonding step Sc2, the first component 10 and the second component 20 are bonded together by pressing them against each other while heating them. Specifically, the first component 10 and the second component 20 are heated to a predetermined temperature in the range of 80 degrees Celsius or higher and 90 degrees Celsius or lower, and then pressed together under a pressure in the range of, for example, 0.002 MPa or higher and 1.2 MPa or lower. In the pressure bonding step Sc2, the epoxy groups (glycidyl groups) of the epoxy silane coupling agent in the first adhesive and the amino groups of the amino silane coupling agent in the second adhesive bond together, thereby curing the first adhesive and the second adhesive to form the bonding layer 30.

[0041] As described above, in this embodiment, a first adhesive is applied to a first surface 11 of a first component 10 made of silicone rubber, while a second adhesive is applied to a second surface 21 of a second component 20 made of polystyrene. The first adhesive comprises a silane coupling agent having epoxy groups (glycidyl groups), while the second adhesive comprises a silane coupling agent having amino groups. Therefore, compared to a configuration where the first component 10 and the second component 20 are bonded to each other using a single type of silane coupling agent, high durability of the bond between the first component 10 and the second component 20 can be maintained.

[0042] In the technology described in Patent Document 1, corona discharge treatment, ultraviolet irradiation treatment, or plasma treatment need to be performed not only before applying the adhesive including the silane coupling agent, but also after application. In contrast to the technology described in Patent Document 1, in the embodiments of this application, the first component 10 and the second component 20 can be firmly bonded together by activating the first surface 11 and the second surface 21 with an excimer beam before applying the adhesive. In other words, it is not necessary to activate the first surface 11 and the second surface 21 after applying the adhesive. Therefore, the manufacturing process of the laminate 100 is simplified compared to the technology described in Patent Document 1.

[0043] Example

[0044] Examples of the invention are described below. Each of the following examples is a corresponding aspect of the invention. Therefore, the scope of the invention is not limited to the examples described below.

[0045] Table 1 is a schematic diagram illustrating the production conditions and evaluation test results of the first to fifth examples according to the present invention. Table 2 is a schematic diagram illustrating the production conditions and evaluation test results of the first to fifth control examples for comparison with each example. In each example and each control example, a PDMS sheet is used as the first component 10, the PDMS sheet being rectangular in shape and having a predetermined size (110 mm × 74 mm), and a polystyrene culture plate is used as the second component 20, the polystyrene culture plate having the same size as the first component 10 (110 mm × 74 mm). In Tables 1 and 2, "PDMS" refers to the first component 10, and "polystyrene" refers to the second component 20.

[0046] Table 1

[0047]

[0048] Table 2

[0049]

[0050]

[0051] In Tables 1 and 2, "glycidyl group" refers to epoxy silane coupling agents containing a glycidyl group. Specifically, 3-glycidoxypropyltrimethoxysilane (product number: DOWSILZ-6040) manufactured by DOW Inc. is used as this epoxy silane coupling agent. In Table 1, "epoxy group" refers to epoxy silane coupling agents containing an epoxy group. Specifically, 2-(3,4-epoxycyclohexyl)ethyltrimethoxysilane (product number: E0327) manufactured by Tokyo Chemical Industry (TCI) Co., Ltd. is used as this epoxy silane coupling agent.

[0052] In Tables 1 and 2, "amino group" refers to aminosilane coupling agents containing an amino group. Specifically, 3-aminopropyltrimethoxysilane (product number: A0439) manufactured by Tokyo Chemical Industries, Ltd. (TCI) is used as the aminosilane coupling agent. In Table 2, "thiol group" refers to silane coupling agents containing a thiol group as a functional group. Specifically, (3-mercaptopropyl)trimethoxysilane (product number: M0928) manufactured by Tokyo Chemical Industries, Ltd. (TCI) is used as the "thiol group" in Table 2.

[0053] Details of each example are as follows.

[0054] First Example

[0055] In the first example 1, an epoxy silane coupling agent (3-glycidoxypropyltrimethoxysilane) having a glycidyl group is applied to the first surface 11 of the first component 10, and a second adhesive comprising an aminosilane coupling agent (3-aminopropyltrimethoxysilane) is applied to the second surface 21 of the second component 20. In each of the first and second adhesives, a mixed solution of ethanol and water in a 9:1 ratio is used as the solvent, and the concentration of the silane coupling agent is set to 1% by weight. Furthermore, with the first component 10 to which the first adhesive is applied and the second component 20 to which the second adhesive is applied heated to 80 degrees Celsius, the first component 10 and the second component 20 are pressed together at a pressure of 1.2 MPa (1 ton).

[0056] Second example

[0057] In the second example, the concentrations of the silane coupling agent in each of the first and second adhesives are changed from 1 wt% in the first example to 5 wt%. In the second example, all conditions except for the silane coupling agent concentration are the same as in the first example.

[0058] Third Example

[0059] In the third example, the concentrations of the silane coupling agent in each of the first and second adhesives are changed from 1 wt% in the first example to 10 wt%. In the third example, all conditions except for the silane coupling agent concentration are the same as in the first example.

[0060] Fourth example

[0061] In the fourth example, the silane coupling agent in the first adhesive is replaced with 2-(3,4-epoxycyclohexyl)ethyltrimethoxysilane, which has an epoxy group. In the fourth example, all conditions are the same as in the first example, except for the silane coupling agent in the first adhesive.

[0062] Fifth example

[0063] In the fifth example, the solvents for the first and second adhesives are replaced with a mixture of isopropanol and water in a 9:1 ratio. In the fifth example, the conditions are the same as in the first example, except for the solvents.

[0064] Details of each comparative example are as follows.

[0065] First comparison example

[0066] In the first comparative example, an adhesive (hereinafter referred to as the "mixed adhesive") using a mixture of an epoxy silane coupling agent (3-glycidoxypropyltrimethoxysilane) and an aminosilane coupling agent (3-aminopropyltrimethoxysilane) was used to bond the first component 10 and the second component 20 together. Specifically, the mixed adhesive was applied to the first surface 11 of the first component 10 and the second surface 21 of the second component 20 to bond the first component 10 and the second component 20 together. In the first comparative example, the conditions other than the adhesive were the same as those in the first example.

[0067] Second comparison example

[0068] In the second control example, the first component 10 and the second component 20 were bonded together using only an aminosilane coupling agent (3-aminopropyltrimethoxysilane). Specifically, the first component 10 and the second component 20 were bonded to each other by an aminosilane coupling agent applied to the second surface 21 of the second component 20. No adhesive was applied to the first surface 11 of the first component 10. In the second control example, all conditions except for the adhesive were the same as in the first example.

[0069] Third comparison example

[0070] In the third control example, the first component 10 and the second component 20 were bonded together using only an epoxy silane coupling agent (3-glycidoxypropyltrimethoxysilane). Specifically, the first component 10 and the second component 20 were bonded together by applying an epoxy silane coupling agent to the first surface 11 of the first component 10. No adhesive was applied to the second surface 21 of the second component 20. In the third control example, the conditions were the same as in the first example, except for the adhesive.

[0071] Fourth comparison example

[0072] In the fourth comparative example, a second adhesive comprising an aminosilane coupling agent (3-aminopropyltrimethoxysilane) is applied to the first surface 11 of the first component 10, and an epoxysilane coupling agent having a glycidyl group (3-glycidoxypropyltrimethoxysilane) is applied to the second surface 21 of the second component 20. In other words, the silane coupling agent applied to the first component 10 in the fourth comparative example is applied to the second component 20 in the first example, and the silane coupling agent applied to the second component 20 in the fourth comparative example is applied to the first component 10 in the first example. In the fourth comparative example, the conditions other than the adhesive are the same as in the first example.

[0073] Fifth comparison example

[0074] In the fifth comparative example, an epoxy silane coupling agent (3-glycidoxypropyltrimethoxysilane) having a glycidyl group was applied to the first surface 11 of the first component 10, and a silane coupling agent ((3-mercaptopropyl)trimethoxysilane) having a thiol group was applied to the second surface 21 of the second component 20. In other words, the type of silane coupling agent applied to the second surface 21 is different from that in the first example. In the fifth comparative example, the conditions are the same as in the first example, except for the adhesive.

[0075] For the examples and control examples described above, evaluation tests were performed to assess durability. These evaluation tests were conducted to assess the durability of the bonding between the first component 10 and the second component 20, and to assess the water resistance of the adhesive. Each evaluation test was performed with the samples according to the examples and control examples described above immersed in water at 50 degrees Celsius. Specifically, an external force (e.g., tensile force) was applied to the sample every 24 hours for 7 days, and it was then determined whether the first component 10 detached from the second component 20. In the table below, "result" indicates whether detachment occurred during the evaluation test.

[0076] As can be seen from Tables 1 and 2, the first component 10 and the second component 20 were separated in the first to fifth comparative examples, while the first component 10 and the second component 20 were not separated in the first to fifth examples.

[0077] By comparing the first control example with the first to fifth examples, it was confirmed that the adhesive, which mixes epoxy silane coupling agents and amino silane coupling agents, cannot adequately guarantee the durability of the bond between the first component 10 and the second component 20. In other words, it was confirmed that the durability of the bond between the first component 10 and the second component 20 can be adequately guaranteed when the epoxy silane coupling agent is applied to only one of the components (the first component 10 and the second component 20, each with a material different from the other component) and when the amino silane coupling agent is applied to only the other component, as in the first to fifth examples.

[0078] By comparing the second and third comparative examples with the first to fifth examples, it was confirmed that the bonding durability between the first component 10 and the second component 20 could not be adequately guaranteed when using either an epoxy silane coupling agent or an amino silane coupling agent alone. In other words, it was confirmed that the bonding durability between the first component 10 and the second component 20 could be adequately guaranteed when using both an epoxy silane coupling agent and an amino silane coupling agent, as shown in the first to fifth examples.

[0079] By comparing the fourth comparative example with the first to fifth examples, it was confirmed that in the configuration where an aminosilane coupling agent is applied to the first surface 11 of the first component 10 made of silicone rubber and an epoxysilane coupling agent is applied to the second surface 21 of the second component 20 made of polystyrene, the durability of the bond between the first component 10 and the second component 20 cannot be sufficiently guaranteed. In other words, it was confirmed that the combination of a first adhesive made of epoxysilane coupling agent applied to the first component 10 made of silicone rubber and a second adhesive made of aminosilane coupling agent applied to the second component 20 made of polystyrene can sufficiently guarantee the bond durability between the first component 10 and the second component 20, as in the first to fifth examples.

[0080] By comparing the fifth comparative example with the first to fifth examples, it was confirmed that in a configuration using a silane coupling agent other than an aminosilane coupling agent as the silane coupling agent applied to the second component 20, the durability of the bond between the first component 10 and the second component 20 cannot be sufficiently guaranteed. In other words, it was confirmed that in a configuration using an aminosilane coupling agent as a second adhesive applied to the second component 20, the durability of the bond between the first component 10 and the second component 20 can be sufficiently guaranteed, as in the first to fifth examples.

[0081] According to the first to third examples, it has been confirmed that in configurations where the concentrations of the silane coupling agents in the first adhesive and the second adhesive are in the range of 1% by weight or more and 10% by weight or less, the durability of the bond between the first component 10 and the second component 20 can be adequately guaranteed.

[0082] According to the fourth example, it has been confirmed that the durability of the bond between the first component 10 and the second component 20 can be adequately guaranteed not only in the configuration in which an epoxy silane coupling agent having a glycidyl group is used as the first adhesive, but also in the configuration in which an epoxy silane coupling agent without a glycidyl group is used as the first adhesive.

[0083] According to the fifth embodiment, it has been confirmed that the durability of the bonding between the first component 10 and the second component 20 can be adequately guaranteed not only in the configuration using a mixed solution of ethanol and water as the solvent for the first adhesive and the second adhesive, but also in the configuration using a mixed solution of isopropanol and water as the solvent for the first adhesive and the second adhesive.

[0084] D: Modify

[0085] The following describes the specific modification patterns that can be applied to each of the above modes. As long as there are no conflicts, you may freely choose two or more modification patterns from the following list and combine them.

[0086] (1) In the above embodiments, the timing relationship between the first step Sa and the second step Sb can be freely chosen. In other words, the second step Sb can be executed after the first step Sa is completed, or alternatively, the first step Sa can be executed after the second step Sb is completed. Some or all of the first step Sa and some or all of the second step Sb can be executed in parallel.

[0087] As can be seen from the above examples, the terms "first" and "second" in this application are merely convenient formal labels used to distinguish elements from each other and have no substantive meaning. Specifically, the terms "first" and "second" do not imply the position of the elements or the order in which the elements are produced. Therefore, they cannot be narrowly interpreted as meaning that the step corresponding to "first" is performed before the step corresponding to "second".

[0088] (2) In the above embodiments, the culture plate used for culturing cells is represented as laminate 100, but by Figure 3 The use of the laminate 100 produced by the manufacturing process shown is not limited to the example above. The shape or size of the first component 10 and the second component 20 can be freely changed according to the intended use of the laminate 100. For example, the through hole 22 described above may not be necessary.

[0089] Explanation of reference numerals in the attached figures

[0090] 100...Laminated sheet, 10...First component, 11...First surface, 20...Second component, 21...Second surface, 22...Through hole, 30...Adhesive layer, 31...First layer, 32...Second layer.

Claims

1. A laminate, comprising: The first component is made of silicone rubber; The second component is made of polystyrene; as well as An adhesive layer that bonds the first surface of the first component and the second surface of the second component together. The adhesive layer includes: A first layer, disposed on the first surface, the first layer being made of a silane coupling agent having epoxy groups; and The second layer is disposed on the second surface and is made of a silane coupling agent having amino groups.

2. A method for producing laminates, the method comprising: First application step: Applying a first adhesive to a first surface of a first component, the first component being made of silicone rubber, the first adhesive being produced by mixing a silane coupling agent having epoxy groups in a solvent; Second application step: Applying a second adhesive to the second surface of a second component made of polystyrene, the second adhesive being produced by mixing a silane coupling agent having amino groups into the solvent; as well as Bonding step: The first surface of the first component and the second surface of the second component are bonded together by a chemical reaction between the epoxy groups in the first adhesive and the amino groups in the second adhesive.

3. The method for producing laminates according to claim 2, in, The first adhesive comprises the silane coupling agent having the epoxy groups, wherein the concentration of the silane coupling agent having the epoxy groups is in the range of more than 1% by weight and less than 10% by weight, and The second adhesive comprises the silane coupling agent having the amino group, wherein the concentration of the silane coupling agent having the amino group is in the range of more than 1% by weight and less than 10% by weight.

4. The method for producing laminates according to claim 2 or 3, further comprising: First activation step: Before the first application step is performed, an excimer beam is projected onto the first surface; as well as Second activation step: Before the second application step is performed, an excimer beam is projected onto the second surface.

5. The method for producing laminates according to claim 2 or 3, wherein, The first adhesive and the second adhesive each comprise a mixed solution of ethanol and water as a solvent.

6. The method for producing laminates according to claim 2 or 3, wherein, The silane coupling agent having the epoxy group is a silane coupling agent having a glycidyl group.

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