A Chinese medicine external acupoint patch for treating obesity and a preparation method and application thereof

Abstract

The application discloses a Chinese medicine external use acupoint patch for treating obesity, which comprises Chinese medicine acupoint hot patching and Chinese medicine acupoint cold patching. The Chinese medicine acupoint hot patching comprises the following raw medicinal materials: atractylodes, atractylodes macrocephala, poria cocos, fried coix seed, dried tangerine or orange peel, cassia twig, asarum and white mustard seed. The Chinese medicine acupoint cold patching comprises the following raw medicinal materials: rhubarb, golden cypress, rhizoma alocasia and borneol. The application adopts specific Chinese medicine compositions to make Chinese medicine acupoint hot patching and Chinese medicine acupoint cold patching, which are respectively applied to the Tian-shu acupoint and the Zu-san-li acupoint and the Qu-chi acupoint. Experimental results show that the patching has obvious regulation effect on the concentration of triglyceride, total cholesterol, free fatty acid and glycerol in the serum of mice, can obviously improve the abnormal blood lipid level of obese mice, and can obviously reduce the subcutaneous and internal fat of mice. The application is external patching, is convenient to use in clinic, and has low adverse reaction.

Description

Technical Field

[0001] This invention relates to a traditional Chinese medicine compound, specifically to a traditional Chinese medicine external acupoint patch for treating obesity, its preparation method, and its application. Background Technology

[0002] Lipid metabolism disorders include obesity, dyslipidemia, and atherosclerotic heart disease, among which the incidence of lipid metabolism disorders, represented by obesity, is increasing.

[0003] Traditional Chinese medicine has unique advantages in treating obesity. Among them, the combination of acupuncture and medicine is an effective clinical method for treating obesity. Current research has found that acupoint intervention at different locations has a specific regulatory effect on fat and is related to the activation of local sympathetic nerves in fat.

[0004] Therefore, it is necessary to develop specific Chinese herbal medicine compositions for application to different acupoints based on the existing foundation in order to achieve the goal of weight loss. Summary of the Invention

[0005] Purpose of the invention: The purpose of this invention is to overcome the shortcomings of the existing technology. This invention uses obese mice fed a high-fat diet as the research subject and intervenes with two different traditional Chinese medicine patches (simulating heat stimulation and cold stimulation respectively) on acupoints on the limbs and abdomen. This achieves the effect of increasing fat heat production through cold and heat stimulation to achieve weight loss, providing a scientific basis and effective target for the combined treatment of obesity with acupuncture and medicine.

[0006] Technical solution: To achieve the above objectives, the technical solution adopted by this invention is as follows:

[0007] A traditional Chinese medicine external acupoint patch for treating obesity, comprising hot application of traditional Chinese medicine acupoints and cold application of traditional Chinese medicine acupoints;

[0008] Traditional Chinese medicine acupoint heat patch includes the following raw materials: Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried Coix lacryma-jobi, Citrus reticulata peel, Cinnamomum cassia twig, Asarum heterotropoides and Sinapis alba seed;

[0009] The cold compress of traditional Chinese medicine acupoints includes the following raw materials: rhubarb, phellodendron bark, acorus tatarinowii, and borneol.

[0010] As a preferred option, the above-mentioned traditional Chinese medicine external acupoint patch for treating obesity includes the following ingredients in a weight ratio of 1-2:1-2:1-2:1-2:1-2:1-2:2-4:2-4:Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried Coix lacryma-jobi, Citrus reticulata peel, Cinnamomum cassia twig, Asarum heterotropoides and Sinapis alba.

[0011] The traditional Chinese medicine acupoint cold patch consists of rhubarb, phellodendron bark, acorus tatarinowii, and borneol in a weight ratio of 1-2:1-2:1-2:2-4.

[0012] As a preferred option, the above-mentioned traditional Chinese medicine external acupoint patch for treating obesity includes the following ingredients in a weight ratio of 1:1:1:1:1:1:2:2: Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried Coix lacryma-jobi, Citrus reticulata peel, Cinnamomum cassia twig, Asarum heterotropoides and Sinapis alba.

[0013] The traditional Chinese medicine acupoint cold patch consists of rhubarb, phellodendron bark, acorus tatarinowii, and borneol in a weight ratio of 1:1:1:2.

[0014] As a preferred option, the above-mentioned traditional Chinese medicine external acupoint patch for treating obesity is applied as follows: hot patch is applied to Tianshu acupoint; cold patch is applied to Zusanli and Quchi acupoints.

[0015] As a preferred embodiment, the preparation method of the above-described traditional Chinese medicine external acupoint patch for treating obesity includes the following steps:

[0016] Take Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried Coix lacryma-jobi, Citrus reticulata peel, Cinnamomum cassia twig, Asarum heterotropoides, and Sinapis alba seeds according to the weight ratio, grind each into powder, sift, mix thoroughly, add ginger juice and liquid petroleum jelly, mix well, make into round pills, and place in hypoallergenic adhesive tape.

[0017] The preparation method of cold compress of traditional Chinese medicine acupoints includes the following steps:

[0018] Take rhubarb, phellodendron bark, acorus tatarinowii and borneol according to the weight ratio, grind them into powder, sift them, mix them thoroughly, add liquid maltose to the mixed powder, mix well and make round pills, and place them in desensitizing adhesive tape.

[0019] As a preferred embodiment, the preparation method of the above-described traditional Chinese medicine external acupoint patch for treating obesity includes the following steps:

[0020] Take the following Chinese herbs according to the weight ratio: Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried coix seed, tangerine peel, cinnamon twig, Asarum heterotropoides, and white mustard seed. Grind each herb into powder, pass them through an 80-mesh sieve, and mix them thoroughly. Add 70mL of fresh ginger juice and 30mL of liquid petroleum jelly to every 110g of mixed herb powder. Mix well and form into round pills with a diameter of 5mm and a weight of about 0.2g. Place the pills in a 1cm×1cm hypoallergenic adhesive tape.

[0021] The preparation method of cold compress of traditional Chinese medicine acupoints includes the following steps:

[0022] Take the Chinese herbs rhubarb, phellodendron bark, acorus tatarinowii, and borneol according to the weight ratio, grind them into powder, pass them through an 80-mesh sieve, mix them thoroughly, add 100mL of liquid maltose to every 110g of mixed powder, mix well, and then make round pills with a diameter of 5mm and a weight of about 0.2g, and place them in a 1cm×1cm desensitizing adhesive tape.

[0023] Beneficial effects: The traditional Chinese medicine external acupoint patch for treating obesity provided by this invention has the following advantages compared with the prior art:

[0024] The formula analysis rules of the traditional Chinese medicine compound compositions screened in this invention are as follows: In the hot compress compound, Atractylodes lancea and Poria cocos are the principal herbs, which invigorate the spleen, dry dampness, and eliminate dampness and turbidity; Atractylodes macrocephala, Citrus reticulata peel, and Coix lacryma-jobi are the assistant herbs, which enhance the spleen-invigorating and dampness-drying effects of the principal herbs; Cinnamomum cassia is the adjuvant herb, which warms and unblocks the meridians and promotes the circulation of qi and blood; Asarum sieboldii and Sinapis alba are the adjuvant herbs, which warm the yang, transform phlegm, promote qi circulation, and disperse stagnation, and together with Cinnamomum cassia, warm and unblock the meridians and stimulate the acupoints. In the cold compress compound, Rheum palmatum is the principal herb, which clears heat from the yangming meridian and promotes the flow of qi and dampness; Phellodendron chinense is the principal herb, which clears heat and dries dampness; Acorus tatarinowii and Borneol are the adjuvant herbs, which transform dampness, harmonize the stomach, clear heat, and stimulate the meridians and acupoints with coldness.

[0025] This invention uses specific Chinese herbal compositions to prepare hot herbal patches for acupoints, which are applied to the Tianshu acupoint, and cold herbal patches for acupoints, which are applied to the Zusanli and Quchi acupoints. The heat stimulation of the Tianshu acupoint on the abdomen can promote the browning of white fat and generate heat, thereby increasing energy consumption. The cold stimulation of the Zusanli and Quchi acupoints can activate the fat sympathetic nerves, promote the decomposition of visceral fat and the heat generation of subcutaneous fat, and further increase energy consumption to achieve the effect of weight loss.

[0026] The experimental results of this invention show that the patch has a significant regulatory effect on the concentrations of triglycerides, total cholesterol, free fatty acids, and glycerol in mouse serum, and can significantly improve abnormal blood lipid levels in obese mice. It also has a significant effect on reducing subcutaneous and visceral fat in mice. This invention is a topical patch, convenient for clinical use, and has few adverse reactions. Attached Figure Description

[0027] Figure 1 Changes in body weight between normal and model mice.

[0028] Figure 2 Changes in body weight of mice in the model and patch application groups.

[0029] Figure 3 Differences in subcutaneous and visceral fat quality among different groups of mice.

[0030] Figure 4 Differences in blood lipids among the groups of mice. Detailed Implementation

[0031] The following examples further illustrate the above-mentioned content of the present invention in detail, but should not be construed as limiting the scope of the subject matter of the present invention to the following examples. All technologies implemented based on the above-mentioned content of the present invention fall within the scope of the present invention.

[0032] Example 1

[0033] 1. A traditional Chinese medicine external acupoint patch for treating obesity, comprising hot application of traditional Chinese medicine acupoints and cold application of traditional Chinese medicine acupoints;

[0034] The herbal acupoint heat patch consists of Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried Coix lacryma-jobi, Citrus reticulata peel, Cinnamomum cassia twig, Asarum heterotropoides and Sinapis alba seeds in a weight ratio of 1:1:1:1:1:1:1:2:2.

[0035] The traditional Chinese medicine acupoint cold patch consists of rhubarb, phellodendron bark, acorus tatarinowii, and borneol in a weight ratio of 1:1:1:2.

[0036] Hot herbal patches are applied to the Tianshu acupoint; cold herbal patches are applied to the Zusanli and Quchi acupoints.

[0037] 2. A method for preparing a traditional Chinese medicine external acupoint patch for treating obesity, the method comprising the following steps:

[0038] Take the following Chinese herbs according to the weight ratio: Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried coix seed, tangerine peel, cinnamon twig, Asarum heterotropoides, and white mustard seed. Grind each herb into powder, pass them through an 80-mesh sieve, and mix them thoroughly. Add 70mL of fresh ginger juice and 30mL of liquid petroleum jelly to every 110g of mixed herb powder. Mix well and form into round pills with a diameter of 5mm and a weight of about 0.2g. Place the pills in a 1cm×1cm hypoallergenic adhesive tape.

[0039] The preparation method of cold compress of traditional Chinese medicine acupoints includes the following steps:

[0040] Take the Chinese herbs rhubarb, phellodendron bark, acorus tatarinowii, and borneol according to the weight ratio, grind them into powder, pass them through an 80-mesh sieve, mix them thoroughly, add 100mL of liquid maltose to every 110g of mixed powder, mix well, and then make round pills with a diameter of 5mm and a weight of about 0.2g, and place them in a 1cm×1cm desensitizing adhesive tape.

[0041] Example 2

[0042] 1. A traditional Chinese medicine external acupoint patch for treating obesity, comprising hot application of traditional Chinese medicine acupoints and cold application of traditional Chinese medicine acupoints;

[0043] The herbal acupoint heat patch consists of Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried Coix lacryma-jobi, Citrus reticulata peel, Cinnamomum cassia twig, Asarum heterotropoides and Sinapis alba seeds in a weight ratio of 2:2:2:2:2:2:4:4.

[0044] The traditional Chinese medicine acupoint cold patch consists of rhubarb, phellodendron bark, acorus tatarinowii, and borneol in a weight ratio of 2:2:2:4.

[0045] Hot herbal patches are applied to the Tianshu acupoint; cold herbal patches are applied to the Zusanli and Quchi acupoints.

[0046] 2. A method for preparing a traditional Chinese medicine external acupoint patch for treating obesity, the method comprising the following steps:

[0047] Take the following Chinese herbs according to the weight ratio: Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried coix seed, tangerine peel, cinnamon twig, Asarum heterotropoides, and white mustard seed. Grind each herb into powder, pass them through an 80-mesh sieve, and mix them thoroughly. Add 70mL of fresh ginger juice and 30mL of liquid petroleum jelly to every 110g of mixed herb powder. Mix well and form into round pills with a diameter of 5mm and a weight of about 0.2g. Place the pills in a 1cm×1cm hypoallergenic adhesive tape.

[0048] The preparation method of cold compress of traditional Chinese medicine acupoints includes the following steps:

[0049] Take the Chinese herbs rhubarb, phellodendron bark, acorus tatarinowii, and borneol according to the weight ratio, grind them into powder, pass them through an 80-mesh sieve, mix them thoroughly, add 100mL of liquid maltose to every 110g of mixed powder, mix well, and then make round pills with a diameter of 5mm and a weight of about 0.2g, and place them in a 1cm×1cm desensitizing adhesive tape.

[0050] Example 3

[0051] 1. Materials and Methods

[0052] 1.1 Laboratory Animals

[0053] Thirty 6-week-old SPF-grade male C57BL / 6 mice, weighing 18-20g, were provided by Zhejiang Vitonlihua Experimental Animal Technology Co., Ltd., production license number: 202108A003. All mice were housed in an SPF-grade environment at the Experimental Animal Center of Nanjing University of Chinese Medicine, with a temperature of 20-24℃, humidity of 45%, and light from 8:00 to 20:00, with free access to food and water. The mice were randomly divided into a normal group (n=10) and a model group (n=20) using a random number table. The normal group was fed a standard diet, while the model group was fed a high-fat diet (HFD) containing 40% lard. All animal handling during the experiment followed the "Guiding Opinions on the Humane Treatment of Laboratory Animals" issued by the Ministry of Science and Technology of the People's Republic of China in 2006.

[0054] 1.2 Modeling Methods and Grouping

[0055] The model group was fed HFD for 10 weeks, and the rats whose body weight exceeded 20% of the body weight of rats fed with normal diet were considered to have successfully established the model. The successfully modeled rats were then randomly divided into a model group and an acupoint application group, with 10 rats in each group. Both groups continued to be fed HFD.

[0056] 1.3 Preparation of acupoint heat patch

[0057] The Chinese herbs Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried Coix lacryma-jobi, Citrus reticulata peel, Cinnamomum cassia, Asarum heterotropoides, and Sinapis alba are ground into powder separately in a ratio of 1:1:1:1:1:1:2:2. They are then passed through an 80-mesh sieve and thoroughly mixed in a clean container. 70 mL of fresh ginger juice and 30 mL of liquid petroleum jelly are added to every 110 g of mixed powder. After mixing well, round pills with a diameter of 5 mm and a weight of about 0.2 g are formed and placed in 1 cm × 1 cm hypoallergenic adhesive tape for later use.

[0058] 1.4 Preparation of cold acupoint application

[0059] Rhubarb, Phellodendron bark, Acorus tatarinowii, and Borneol were each ground into powder in a ratio of 1:1:1:2. The powders were then passed through an 80-mesh sieve and thoroughly mixed in a clean container. 100 mL of liquid maltose was added to every 110 g of the mixed powder. After mixing well, the powders were formed into round pills with a diameter of 5 mm and a weight of about 0.2 g. The pills were then placed in 1 cm × 1 cm hypoallergenic adhesive tape for later use.

[0060] 1.5 Intervention Methods

[0061] In the treatment group, the Tianshu acupoint (located 3 mm lateral to the 7 / 12 and 5 / 12 points below the line connecting the xiphoid process and pubic symphysis) was treated with heat therapy, while the Zusanli acupoint (5 mm lateral to the anterior tubercle of the tibia and 20 mm below the knee joint) and the Quchi acupoint (located at the midpoint between the elbow crease and the lateral epicondyle of the humerus) were treated with cold therapy. The interventions were performed once daily for one hour each time, six times a week, for two weeks.

[0062] 2 Experimental Results

[0063] 2.1 Patch therapy reduces the weight of obese mice

[0064] like Figure 1 As shown, after 10 weeks of high-fat diet feeding, the body weight of mice was significantly higher than that of mice in the normal diet group (P < 0.05), and the average body weight of obese mice exceeded that of normal mice by more than 20%, indicating successful model establishment. Figure 2 As shown, after several weeks of treatment with the patch, the body weight of the patch group decreased significantly, while the body weight of the model group mice continued to increase (P<0.05). The results suggest that the patch therapy significantly reduced the body weight of obese mice.

[0065] 2.2 Patch therapy reduces fat weight in obese mice

[0066] like Figure 3 The results showed that after high-fat feeding, the subcutaneous fat mass and visceral fat weight of the model group mice were significantly higher than those of the normal group (P<0.05). After two weeks of patch intervention, the subcutaneous and visceral fat weights of the mice were significantly reduced (P<0.05). The results suggest that the patch has a significant effect on reducing subcutaneous and visceral fat in mice.

[0067] 2.3 Patch therapy improves blood lipid levels in obese mice

[0068] like Figure 4 As shown: (1) Triglyceride concentration: The serum triglyceride level of the model group mice was significantly higher than that of the normal diet group mice (P<0.05), and it was significantly reduced after the patch treatment intervention (P<0.05). (2) Total cholesterol concentration: The serum total cholesterol concentration of the model group mice was significantly higher than that of the normal group mice (P<0.05), and it was significantly reduced after the patch treatment intervention (P<0.05). (3) Free fatty acid concentration: The serum free fatty acid concentration of the model group mice was significantly higher than that of the normal group (P<0.05), and it was significantly reduced after the patch treatment intervention (P<0.05). (4) Glycerol concentration: The serum glycerol concentration of the model group mice was significantly higher than that of the normal group mice (P<0.05), and it was significantly reduced after the patch treatment intervention (P<0.05).

[0069] In summary, the patch prepared in this invention has a significant regulatory effect on the concentrations of triglycerides, total cholesterol, free fatty acids, and glycerol in mouse serum. Therefore, the weight-loss patch can significantly improve abnormal blood lipid levels in obese mice.

Claims

1. A traditional Chinese medicine external acupoint patch for treating obesity, characterized in that, It consists of hot and cold applications of traditional Chinese medicine acupoint patches; The herbal acupoint heat patch is made from a herbal composition with a mass-to-volume ratio of 11:7 g / mL and ginger juice; The traditional Chinese medicine composition is made from Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried Coix lacryma-jobi, Citrus reticulata peel, Cinnamomum cassia twig, Asarum heterotropoides and Sinapis alba seeds in a weight ratio of 1~2:1~2:1~2:1~2:1~2:1~2:2~4:2~4. The traditional Chinese medicine acupoint cold patch is made from rhubarb, phellodendron bark, acorus tatarinowii and borneol in a weight ratio of 1~2:1~2:1~2:2~4. Traditional Chinese medicine acupoint heat patch is applied to the Tianshu acupoint. The herbal acupoint cold patch is applied to Zusanli and Quchi acupoints.

2. The traditional Chinese medicine external acupoint patch for treating obesity according to claim 1, characterized in that, The herbal composition for hot compress of acupoints is made from Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried Coix lacryma-jobi, Citrus reticulata peel, Cinnamomum cassia twig, Asarum heterotropoides and Sinapis alba seeds in a weight ratio of 1:1:1:1:1:1:1:2:

2. The traditional Chinese medicine acupoint cold patch is made from rhubarb, phellodendron bark, acorus tatarinowii and borneol in a weight ratio of 1:1:1:

2.

3. The preparation method of the traditional Chinese medicine external acupoint patch for treating obesity as described in claim 1 or 2, characterized in that, The preparation method of traditional Chinese medicine acupoint heat patch includes the following steps: Take Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried Coix lacryma-jobi, Citrus reticulata peel, Cinnamomum cassia twig, Asarum heterotropoides, and Sinapis alba seeds according to the weight ratio, grind each into powder, sift, mix thoroughly, add ginger juice and liquid petroleum jelly, mix well, make into round pills, and place in desensitizing adhesive tape. The preparation method of cold compress of traditional Chinese medicine acupoints includes the following steps: Take rhubarb, phellodendron bark, acorus tatarinowii and borneol according to the weight ratio, grind them into powder, sift them, mix them thoroughly, add liquid maltose to the mixed powder, mix well and make round pills, and place them in desensitizing adhesive tape.

4. The preparation method of the traditional Chinese medicine external acupoint patch for treating obesity according to claim 3, characterized in that, The preparation method of traditional Chinese medicine acupoint heat patch includes the following steps: Take the following Chinese herbs according to the weight ratio: Atractylodes lancea, Atractylodes macrocephala, Poria cocos, stir-fried Coix lacryma-jobi, Citrus reticulata peel, Cinnamomum cassia, Asarum heterotropoides, and Sinapis alba. Grind each herb into powder, pass them through an 80-mesh sieve, and mix them thoroughly. Add 70 mL of fresh ginger juice and 30 mL of liquid petroleum jelly to every 110 g of mixed herb powder. Mix well and form round pills with a diameter of 5 mm and a weight of about 0.2 g. Place the pills in a 1 cm × 1 cm hypoallergenic adhesive tape. The preparation method of cold compress of traditional Chinese medicine acupoints includes the following steps: Take the Chinese herbs rhubarb, phellodendron bark, acorus tatarinowii, and borneol according to the weight ratio, grind them into powder, pass them through an 80-mesh sieve, mix them thoroughly, add 100 mL of liquid maltose to every 110 g of mixed powder, mix well, and then make round pills with a diameter of 5 mm and a weight of about 0.2 g, and place them in a 1 cm × 1 cm desensitizing adhesive tape.

5. The use of the traditional Chinese medicine external acupoint patch as described in claim 1 or 2 in the preparation of external preparations for treating hyperlipidemia.

6. The use of the traditional Chinese medicine external acupoint patch as described in claim 1 or 2 in the preparation of external preparations for treating obesity.

Images