A cell sorting device for flow cytometry

Abstract

The present invention provides a cell sorting device for flow cytometry detection, which relates to the field of cell sorting technology, including a sorting box and a sorting test tube, wherein the sorting box is provided with a detection mechanism for liquid level detection of the sorting test tube, the sorting box is provided with a fixing mechanism for fixing the sorting test tube, the sorting box is provided with a driving mechanism for replacing the sorting test tube, the detection mechanism includes a fluid device, a laser irradiation device, a charge coupled device and an ultrasonic detector, the fixing mechanism includes a test tube rack and a pull rod, and the driving mechanism includes an adjusting rod and a circular plate. The present invention cooperates with an ultrasonic detector, a circular plate and a valve plate, and the ultrasonic detector detects the liquid level in the sorting test tube in real time. When the liquid level threshold exceeds the set value, the valve plate is driven to rotate and close, so as to prevent the liquid from continuing to fall and causing the liquid level in the sorting test tube to overflow, resulting in waste of cell suspension.

Description

Technical Field

[0001] The present invention belongs to the technical field of cell sorting, and more specifically, particularly relates to a cell sorting device for flow cytometry detection. Background Art

[0002] The cell sorting device for flow cytometry is an efficient cell analysis and sorting tool. It is a method of detecting and analyzing a cell suspension as a single cell by passing it through a laser beam. The cell sample is made into a single-cell suspension and specific cell components are labeled with fluorescent dyes. The cell suspension passes through a laser beam as a single cell through a fluid system. Usually, a charge-coupled device or acoustic sorting technology is used to guide specific cells into different collection containers by electric charge or sound waves.

[0003] The Chinese patent publication number is: CN115791576A, a cell flow sorting device, which realizes the detection, sorting and distribution of circulating tumor cells in whole blood, and finally obtains circulating tumor cells wrapped in a single droplet. While realizing the counting of circulating tumor cells, cells with higher activity can be obtained for subsequent biological detection; and the target single cell can be directly obtained by whole blood sampling, and the manual operation is simple.

[0004] Existing cell flow sorting devices have the following disadvantages:

[0005] 1. In the sorting of cell suspension, too fast flow rate and long sorting time may cause the liquid level in the test tube to be too high and liquid overflow. Some existing sorting devices are not equipped with alarm devices, and staff are required to observe the liquid level of the test tube in real time, which is more labor-intensive;

[0006] 2. Generally, an alarm device is used to remind the staff to change the liquid level when it reaches a certain threshold. However, the staff may not respond in time or fail to pay attention to the alarm, causing the device to continue to sort the cell suspension, which in turn causes overflow and waste;

[0007] 3. When replacing test tubes, the existing sorting device usually needs to take out the entire test tube rack and then replace the test tube with a higher liquid level, which may contaminate the suspension in other test tubes, thereby affecting the accuracy of detection and sorting.

[0008] Therefore, in view of this, the existing structure and defects are studied and improved, and a cell sorting device for flow cytometry is provided, in order to achieve a more practical purpose. Summary of the invention

[0009] In order to solve the above technical problems, the present invention provides a cell sorting device for flow cytometry detection to solve the above problems.

[0010] A cell sorting device for flow cytometry detection comprises a sorting box and a sorting test tube, wherein the sorting box is provided with a detection mechanism for conveniently detecting the liquid level of the sorting test tube, the sorting box is provided with a fixing mechanism for conveniently fixing the sorting test tube, the sorting box is provided with a driving mechanism for conveniently replacing the sorting test tube, the detection mechanism comprises a fluid device, a laser irradiation device, a charge coupled device and an ultrasonic detector, the fixing mechanism comprises a test tube rack and a pull rod, the driving mechanism comprises an adjusting rod and a circular plate, the fluid device is fixedly mounted on the inner side wall of the sorting box, the laser irradiation device The emitting device is fixedly mounted on the two side ends of the inner wall of the sorting box, the charge coupled device is fixedly mounted on the two side ends of the inner wall of the sorting box, two first telescopic rods are fixedly mounted between the adjusting rod and the inner wall of the sorting box, the test tube rack is slidably mounted on the inner wall of the sorting box, a plurality of placement slots are opened on the upper end of the test tube rack, the number of the pull rods is multiple, each of the pull rods is respectively located on the inner wall of the placement slot, each of the ultrasonic detectors is fixedly mounted on the inner wall of the placement slot, the lower end of the inner wall of the sorting box is provided with two rectangular slide slots, the lower end of the test tube rack The inner wall of the fluid device is rotatably mounted with a valve plate, and the side end of the valve plate is fixedly mounted with a rotating shaft, and the rotating shaft penetrates and is rotatably mounted on the side end of the fluid device, and a gear is fixedly mounted on the circumferential end of the rotating shaft, and a rack is fixedly mounted on the upper end of the adjusting rod, and the rack and the gear are meshed, and a C-shaped rod is fixedly mounted on the side end of the test tube rack, and a T-shaped slot is provided on the side end of each pull rod, and rubber pads are fixedly mounted on both side ends of each pull rod, A first spring is fixedly installed between each pull rod and the C-shaped rod, two clamping plates are provided on one side of each pull rod, a T-shaped slider is fixedly installed on the side end of each clamping plate, two second springs are fixedly installed between every two T-shaped sliders, a connecting rod is fixedly installed on the side end of each clamping plate, a clamping rod is fixedly installed on the side end of each connecting rod, the same control device is fixedly installed on the upper end of each ultrasonic detector, a plurality of motors are fixedly installed on the upper end of the control device, and a threaded rod is fixedly installed on the output end of each motor.

[0011] Preferably, there are multiple circular plates, a thread groove is penetrated through the upper end of each circular plate, each threaded rod is threadedly installed on the inner wall of each thread groove, and a side push rod is fixedly installed on the side end of each circular plate.

[0012] Preferably, a second telescopic rod is fixedly installed between each of the side push rods and the inner wall of the sorting box, an L-shaped connecting rod is fixedly installed on the circumferential end of each of the circular plates, and a clamping plate is fixedly installed on the lower end of each of the L-shaped connecting rods.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] In the present invention, an ultrasonic detector, a circular plate and a valve plate are arranged in coordination, and the ultrasonic detector is used to detect the liquid level in the sorting test tube in real time. When the liquid level threshold exceeds the set value, the valve plate is driven to rotate and close with the coordination of the circular plate, the rack and the gear, so as to prevent the liquid from continuing to fall and causing the liquid level in the sorting test tube to overflow, resulting in waste of cell suspension and affecting the sorting efficiency.

[0015] In the present invention, an ultrasonic detector and a motor are arranged in cooperation. When the existing device is used, after the liquid level position exceeds the threshold, the staff is generally reminded to replace the sorting test tube through an alarm device. However, when the liquid level position of the present device exceeds the threshold, the valve plate can be directly driven to rotate and close, thereby avoiding the problem of liquid overflow caused by untimely operation of the device due to untimely reminders or untimely response of the staff.

[0016] In the present invention, by arranging the coupling rod, the coupling plate and the L-shaped connecting rod to cooperate with each other, after the liquid level of the corresponding sorting test tube exceeds the threshold value, while the side push rod drives the valve plate upward to close, it can drive the coupling plate to move upward so that the coupling plate no longer limits the coupling rod, which makes it convenient for the first spring to pull the pull rod to move, and drive the sorting test tube to leave the placement slot on the test tube rack, so that the user can replace the sorting test tube in time.

[0017] In the present invention, by arranging the pull rod, the clamping plate and the first spring to cooperate with each other, when the liquid level in a certain sorting test tube is too high and the corresponding sorting test tube needs to be replaced, the corresponding first spring will pull the clamping plate to drive the sorting test tube with too high liquid level to leave the placement slot, and then the user can take out the corresponding sorting test tube for replacement without taking out the entire test tube rack for replacement, avoiding taking out the entire test tube rack, which may contaminate the cell suspension in other sorting test tubes.

[0018] In the present invention, rubber pads are provided at both end portions of the pull rod. When the first spring pulls the pull rod to move, a certain resistance can be generated by the friction between the rubber pads and the inner wall of the placement groove, so that the valve plate can smoothly pull out the sorting test tube in the clamping plate when it rebounds, avoiding the situation where the liquid in the sorting test tube overflows due to excessive rebound pulling force of the first spring, thereby ensuring the stability of the sorting test tube when the sorting test tube is replaced. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1It is a schematic diagram of the overall structure of the sorting box of the present invention;

[0020] Figure 2 It is a schematic diagram of the overall structure of the fluid device of the present invention;

[0021] Figure 3 It is a schematic diagram of the overall structure of the valve plate of the present invention;

[0022] Figure 4 It is a schematic diagram of the overall structure of the test tube rack of the present invention;

[0023] Figure 5 It is a schematic diagram of the overall structure of the pull rod of the present invention;

[0024] Figure 6 It is a schematic diagram of the overall structure of the clamping plate of the present invention;

[0025] Figure 7 It is a schematic diagram of the overall structure of the ultrasonic detector of the present invention;

[0026] Figure 8 It is a schematic structural diagram of the entire clamping plate of the present invention.

[0027] In the figure, the corresponding relationship between the component names and the figure numbers is: 1. sorting box; 11. laser irradiation device; 12. charge coupled device; 13. rectangular slide; 2. fluid device; 21. valve plate; 22. rotating shaft; 23. gear; 3. adjusting rod; 31. rack; 32. first telescopic rod; 4. test tube rack; 41. rectangular slide bar; 42. C-shaped rod; 43. placement groove; 5. pull rod; 51. rubber pad; 52. first spring; 53. clamping plate; 54. T-shaped slider; 55. second spring; 56. connecting rod; 57. clamping rod; 58. T-shaped slide; 6. ultrasonic detector; 61. control device; 62. motor; 63. threaded rod; 7. round plate; 71. threaded groove; 72. second telescopic rod; 73. side push rod; 74. L-shaped connecting rod; 75. clamping plate; 8. sorting test tube. DETAILED DESCRIPTION

[0028] The following embodiments of the present invention are described in further detail in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0029] See also Figure 1-Figure 8The present invention provides a cell sorting device for flow cytometry detection, comprising a sorting box 1 and a sorting test tube 8, wherein the sorting box 1 is provided with a detection mechanism for conveniently detecting the liquid level of the sorting test tube 8, the sorting box 1 is provided with a fixing mechanism for conveniently fixing the sorting test tube 8, the sorting box 1 is provided with a driving mechanism for conveniently replacing the sorting test tube 8, the detection mechanism comprises a fluid device 2, a laser irradiation device 11, a charge coupled device 12 and an ultrasonic detector 6, the fixing mechanism comprises a test tube rack 4 and a pull rod 5, the driving mechanism comprises an adjusting rod 3 and a circular plate 7, the fluid device 2 is fixedly mounted on the inner side wall of the sorting box 1, the laser irradiation device 11 is provided with a fixing mechanism for conveniently fixing the sorting test tube 8, the driving mechanism comprises ... The device 11 is fixedly mounted on both ends of the inner wall of the sorting box 1, and the charge coupled device 12 is fixedly mounted on both ends of the inner wall of the sorting box 1. When sorting cells, the cell sample is made into a single cell suspension, and specific cell components are marked with fluorescent dyes. The cell suspension drips through the fluid device 2. Ideally, each drop of liquid contains a single cell. The cell suspension passes through the laser irradiation device 11 in the form of a single cell. The laser beam emitted by the laser irradiation device 11 irradiates each cell. After irradiation, when the cell suspension passes through the charge coupled device 12, the specific cells are guided into different sorting test tubes 8 by electric charges or sound waves.

[0030] Two first telescopic rods 32 are fixedly installed between the adjusting rod 3 and the inner wall of the sorting box 1, the test tube rack 4 is slidably installed on the inner wall of the sorting box 1, and a plurality of placement slots 43 are provided at the upper end of the test tube rack 4. There are multiple pull rods 5, and each pull rod 5 is respectively located on the inner wall of the placement slot 43. Each ultrasonic detector 6 is fixedly installed on the inner wall of the placement slot 43. Two rectangular slides 13 are provided at the lower end of the inner wall of the sorting box 1. Two rectangular slides 41 are fixedly installed at the lower end of the test tube rack 4. The two rectangular slides 41 are respectively slidably installed on the inner walls of the two rectangular slides 13. A valve plate 21 is rotatably installed on the inner wall of the fluid device 2. A rotating shaft 22 is fixedly installed on the side end of the valve plate 21. The rotating shaft 22 passes through and is rotatably installed on the side end of the fluid device 2. A gear 23 is fixedly installed on the circumferential end of the rotating shaft 22. The upper end of the adjusting rod 3 A rack 31 is fixedly installed on the part, and the rack 31 is meshed with the gear 23. Each ultrasonic detector 6 will monitor the liquid level of the corresponding sorting test tube 8 in real time. When the liquid level of one of the sorting test tubes 8 exceeds a certain threshold, the ultrasonic detector 6 will detect it and feedback an electrical signal to the control device 61. The control device 61 will drive the corresponding motor 62 to start. The start of the motor 62 will drive the threaded rod 63 to rotate. The rotation of the threaded rod 63 will drive the circular plate 7 to move upward through the thread groove 71. The upward movement of the circular plate 7 drives the side push rod 73 to move upward. When the side push rod 73 moves upward and contacts the adjusting rod 3, it will drive the adjusting rod 3 to move upward. The adjusting rod 3 moves upward and the rack 31 moves upward. The upward movement of the rack 31 will drive the gear 23 to rotate. The rotation of the gear 23 drives the valve plate 21 to rotate through the rotating shaft 22. The rotation of the valve plate 21 gradually closes the fluid device 2.

[0031] A C-shaped rod 42 is fixedly installed on the side end of the test tube rack 4, and a T-shaped slide groove 58 is opened on the side end of each pull rod 5. Rubber pads 51 are fixedly installed on both side ends of each pull rod 5. By arranging rubber pads 51 on both side ends of the pull rod 5, when the first spring 52 pulls the pull rod 5 to move, a certain resistance can be generated by the friction between the rubber pad 51 and the inner wall of the placement groove 43, so that when the valve plate 21 rebounds, the sorting test tube 8 in the clamping plate 53 can be smoothly pulled out, avoiding the situation where the liquid in the sorting test tube 8 overflows due to excessive rebound tension of the first spring 52, thereby ensuring the stability of the sorting test tube 8 when the sorting test tube 8 is replaced;

[0032] A first spring 52 is fixedly installed between each pull rod 5 and the C-shaped rod 42, two clamping plates 53 are provided on one side of each pull rod 5, a T-shaped slider 54 is fixedly installed on the side end of each clamping plate 53, two second springs 55 are fixedly installed between every two T-shaped sliders 54, a connecting rod 56 is fixedly installed on the side end of each clamping plate 53, a clamping rod 57 is fixedly installed on the side end of each connecting rod 56, the same control device 61 is fixedly installed on the upper end of each ultrasonic detector 6, a plurality of motors 62 are fixedly installed on the upper end of the control device 61, a threaded rod 63 is fixedly installed on the output end of each motor 62, there are multiple circular plates 7, a threaded groove 71 is penetrated through the upper end of each circular plate 7, each threaded rod 63 is respectively threadedly installed on the inner side wall of each threaded groove 71, a side push rod 73 is fixedly installed on the side end of each circular plate 7, and each side push rod 73 and the sorting The second telescopic rod 72 is fixedly installed between the inner side walls of the box body 1, and the circumferential end of each circular plate 7 is fixedly installed with an L-shaped connecting rod 74, and the lower end of each L-shaped connecting rod 74 is fixedly installed with a clamping plate 75. When the circular plate 7 on the corresponding sorting test tube 8 moves upward, it will also drive the L-shaped connecting rod 74 to move upward. The upward movement of the L-shaped connecting rod 74 will gradually drive the clamping plate 75 to move upward. After the circular plate 7 moves upward and drives the valve plate 21 to rotate and close completely, the clamping plate 75 moves upward and completely leaves the side end of the clamping rod 57, that is, the clamping rod 57 is no longer limited. At this time, the first spring 52 is no longer subjected to tension and will rebound. The first spring 52 rebounds and drives the pull rod 5 to move. The pull rod 5 moves and drives the clamping plate 53 to move through the T-shaped slider 54. The movement of the clamping plate 53 will drive the sorting test tube 8 whose liquid level exceeds the threshold to move, and then drive the sorting test tube 8 to leave the test tube rack 4, which is convenient for the user to replace the sorting test tube 8.

[0033] Working principle:

[0034] In the first step, when sorting cells, the cell sample is made into a single cell suspension, and specific cell components are marked with fluorescent dyes. The cell suspension drips through the fluid device 2. Ideally, each drop of liquid contains a single cell. The cell suspension passes through the laser irradiation device 11 in the form of a single cell. The laser beam emitted by the laser irradiation device 11 irradiates each cell. After irradiation, when the cell suspension passes through the charge coupled device 12, the specific cells are guided into different sorting test tubes 8 by electric charges or sound waves. When the liquid level of a sorting test tube 8 in the test tube rack 4 is high, it is detected by the ultrasonic detector 6. The ultrasonic detector 6 drives the corresponding motor 62 through the control device 61 to start and drive the circular plate 7 to move upward. Under the cooperation of the rack 31 and the gear 23, the valve plate 21 is driven to rotate and close the fluid device 2. At this time, the cell suspension no longer drips from the fluid device 2. At the same time, the clamping plate 75 moves away from one side of the clamping rod 57. The first spring 52 pulls the two clamping plates 53 to move, driving the corresponding sorting test tube 8 to move out of the test tube rack 4, and the user can replace it.

[0035] In the second step, during the sorting process of the cell suspension, the cell suspension containing different single cells gradually falls into different sorting test tubes 8, and the cell suspension is sorted and collected. The speed of the liquid level rising in each sorting test tube 8 is different. Each ultrasonic detector 6 will monitor the liquid level of the corresponding sorting test tube 8 in real time. When the liquid level of one of the sorting test tubes 8 exceeds a certain threshold, the ultrasonic detector 6 will detect it and feedback an electrical signal to the control device 61, and the control device 61 will drive the corresponding motor 62 to start, and the start of the motor 62 will drive the thread The rod 63 rotates, and the rotation of the threaded rod 63 drives the circular plate 7 to move upward through the threaded groove 71. The upward movement of the circular plate 7 drives the side push rod 73 to move upward. When the side push rod 73 moves upward and contacts the adjusting rod 3, it drives the adjusting rod 3 to move upward. The adjusting rod 3 moves upward and the rack 31 moves upward. The upward movement of the rack 31 drives the gear 23 to rotate. The rotation of the gear 23 drives the valve plate 21 to rotate through the rotating shaft 22. The rotation of the valve plate 21 gradually closes the fluid device 2, so that the cell suspension stops dripping, and the situation of liquid overflow caused by continued dripping is avoided;

[0036] The device is provided with an ultrasonic detector 6, a circular plate 7 and a valve plate 21, and the ultrasonic detector 6 detects the liquid level in the sorting test tube 8 in real time. When the liquid level threshold exceeds the set value, the circular plate 7, the rack 31 and the gear 23 cooperate to drive the valve plate 21 to rotate and close, so as to prevent the liquid from continuing to fall and causing the liquid level in the sorting test tube 8 to overflow, causing a waste of cell suspension, and avoiding affecting the efficiency of sorting.

[0037] The device is provided with an ultrasonic detector 6 and a motor 62. When the existing device is used, after the liquid level exceeds the threshold, the alarm device is generally used to remind the staff to replace the sorting test tube 8. However, after the liquid level exceeds the threshold, the valve plate 21 can be directly driven to rotate and close, thereby avoiding the problem of liquid overflow caused by untimely reminder or untimely response of the staff, resulting in untimely operation of the device.

[0038] In the third step, when the circular plate 7 on the corresponding sorting test tube 8 moves upward, it will also drive the L-shaped connecting rod 74 to move upward. The upward movement of the L-shaped connecting rod 74 will gradually drive the clamping plate 75 to move upward. After the circular plate 7 moves upward to drive the valve plate 21 to rotate and close completely, the clamping plate 75 moves upward and completely leaves the side end of the clamping rod 57, that is, the clamping rod 57 is no longer limited. At this time, the first spring 52 is no longer subjected to tension and will rebound. The rebound of the first spring 52 drives the pull rod 5 to move. The movement of the pull rod 5 drives the clamping plate 53 to move through the T-shaped slider 54. The movement of the clamping plate 53 will drive the sorting test tube 8 whose liquid level exceeds the threshold to move, and then drive the sorting test tube 8 to leave the test tube rack 4, so that the user can replace the sorting test tube 8.

[0039] The device cooperates with the clamping rod 57, the clamping plate 75 and the L-shaped connecting rod 74. After the liquid level of the corresponding sorting test tube 8 exceeds the threshold value, the side push rod 73 drives the valve plate 21 upward to close, and at the same time, the clamping plate 75 can be driven to move upward so that the clamping plate 75 no longer limits the clamping rod 57, so that the first spring 52 pulls the pull rod 5 to move, and drives the sorting test tube 8 to leave the placement slot 43 on the test tube rack 4, so that the user can replace the sorting test tube 8 in time.

[0040] The device is provided with a pull rod 5, a clamping plate 53 and a first spring 52, and when the liquid level in a certain sorting test tube 8 is too high and the corresponding sorting test tube 8 needs to be replaced, the corresponding first spring 52 will pull the clamping plate 53 to drive the sorting test tube 8 with the excessively high liquid level to leave the placement slot 43, and then the user can take out the corresponding sorting test tube 8 for replacement, without taking out the entire test tube rack 4 for replacement, so as to avoid taking out the entire test tube rack 4, which may contaminate the cell suspension in other sorting test tubes 8;

[0041] In this device, rubber pads 51 are arranged on both side ends of the pull rod 5. When the first spring 52 pulls the pull rod 5 to move, a certain resistance can be generated by the friction between the rubber pads 51 and the inner wall of the placement groove 43, so that when the valve plate 21 rebounds, the sorting test tube 8 in the clamping plate 53 can be pulled out smoothly, avoiding the situation where the liquid in the sorting test tube 8 overflows due to excessive rebound tension of the first spring 52, thereby ensuring the stability of the sorting test tube 8 when the sorting test tube 8 is replaced.

[0042] The embodiments of the present invention are given for the purpose of illustration and description, and are not intended to be exhaustive or to limit the invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.

Claims

1. A cell sorting device for flow cytometry, comprising a sorting box (1) and a sorting test tube (8), characterized in that: The sorting box (1) is provided with a detection mechanism for facilitating liquid level detection of the sorting test tube (8), the sorting box (1) is provided with a fixing mechanism for facilitating fixing the sorting test tube (8), and the sorting box (1) is provided with a driving mechanism for facilitating replacement of the sorting test tube (8); The detection mechanism comprises a fluid device (2), a laser irradiation device (11), a charge coupled device (12) and an ultrasonic detector (6); the fixing mechanism comprises a test tube rack (4) and a pull rod (5); the driving mechanism comprises an adjustment rod (3) and a circular plate (7); the fluid device (2) is fixedly mounted on the inner wall of the sorting box (1); the laser irradiation device (11) is fixedly mounted on both side ends of the inner wall of the sorting box (1); the charge coupled device (12) is fixedly mounted on the inner wall of the sorting box (1); Two first telescopic rods (32) are fixedly installed between the adjusting rod (3) and the inner wall of the sorting box (1) at the two side ends of the inner wall of the box (1), the test tube rack (4) is slidably installed on the inner wall of the sorting box (1), and a plurality of placement grooves (43) are opened at the upper end of the test tube rack (4). There are a plurality of pull rods (5), each of which is located on the inner wall of the placement groove (43), and each of the ultrasonic detectors (6) is fixedly installed on the inner wall of the placement groove (43); A valve plate (21) is rotatably mounted on the inner side wall of the fluid device (2), a rotating shaft (22) is fixedly mounted on the side end of the valve plate (21), the rotating shaft (22) penetrates and is rotatably mounted on the side end of the fluid device (2), a gear (23) is fixedly mounted on the circumferential end of the rotating shaft (22), a rack (31) is fixedly mounted on the upper end of the adjusting rod (3), the rack (31) and the gear (23) are meshed, a C-shaped rod (42) is fixedly mounted on the side end of the test tube rack (4), a T-shaped groove (58) is provided on the side end of each pull rod (5), and a rubber pad (51) is fixedly mounted on both side ends of each pull rod (5); A first spring (52) is fixedly installed between each of the pull rods (5) and the C-shaped rod (42), two clamping plates (53) are provided on one side of each of the pull rods (5), a T-shaped slider (54) is fixedly installed on the side end of each of the clamping plates (53), two second springs (55) are fixedly installed between each two of the T-shaped sliders (54), a connecting rod (56) is fixedly installed on the side end of each of the clamping plates (53), and a connecting rod (56) is fixedly installed on the side end of each of the connecting rods (56). A clamping rod (57) is provided, the upper end of each ultrasonic detector (6) is fixedly mounted with a same control device (61), the upper end of the control device (61) is fixedly mounted with a plurality of motors (62), the output end of each motor (62) is fixedly mounted with a threaded rod (63), the number of the circular plates (7) is multiple, the upper end of each circular plate (7) is penetrated by a threaded groove (71), and each threaded rod (63) is respectively threadedly mounted on the inner side wall of each threaded groove (71); A side push rod (73) is fixedly mounted on the side end of each circular plate (7), a second telescopic rod (72) is fixedly mounted between each side push rod (73) and the inner wall of the sorting box (1), an L-shaped connecting rod (74) is fixedly mounted on the circumferential end of each circular plate (7), and a clamping plate (75) is fixedly mounted on the lower end of each L-shaped connecting rod (74).

2. A cell sorting device for flow cytometry as claimed in claim 1, characterized in that: The lower end of the inner wall of the sorting box (1) is provided with two rectangular slide grooves (13), and the lower end of the test tube rack (4) is fixedly mounted with two rectangular slide bars (41), and the two rectangular slide bars (41) are respectively slidably mounted on the inner walls of the two rectangular slide grooves (13).

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