Preparation process of hydrolyzed keratin and centrifugal filtration device thereof

Abstract

The present invention relates to the technical field of bioengineering, specifically to a preparation process for hydrolyzed keratin and a centrifugal filter device thereof. The preparation process comprises the following steps: S1: pretreatment; S2: oxidation treatment; S3: enzymatic hydrolysis; and S4: purification. The centrifugal filter device is prepared, comprising an organism, a centrifugal tank being provided on the organism, an organic top cover being provided above the centrifugal tank, and a drive motor being installed on the top cover. The preparation process for hydrolyzed keratin and the centrifugal filter device thereof proposed by the present invention are safe, simple, convenient, and economical in production process, and are easy to industrialize and mass-produce. The molecular weight range of the hydrolyzed keratin produced by the process is relatively small, at about 500-3000, and the molecular weight is relatively small. The hydrolyzed keratin produced by the process can regulate its solid content, meet the requirements of regulating its pH value and regulating its relative molecular weight, etc. During preparation, the device can simultaneously perform centrifugal filtration, is convenient to operate, and improves production efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of bioengineering, specifically to the preparation process of hydrolyzed keratin and its centrifugal filtration device. Background Art

[0002] Keratin is one of the key factors for hair health. If hair lacks keratin, problems such as dryness, fragility, frizz, and stubbornness will occur. Therefore, maintaining appropriate keratin intake and correct care methods can help hair stay healthy and shiny. Among them, keratin hair care is relatively common in life. Hair care products containing keratin can repair curls and improve the gloss of hair. Hair keratin is generally prepared from wool and feathers as raw materials to prepare keratin solutions. When preparing keratin, a hydrolyzed keratin concentrate needs to be obtained. However, the existing production process is relatively troublesome, costly, and the range of protein molecular weights produced is relatively large, and improvements are needed. Summary of the Invention

[0003] The purpose of the present invention is to provide a preparation process of hydrolyzed keratin and its centrifugal filtration device to solve the problems raised in the above background art.

[0004] To achieve the above purpose, the present invention provides the following technical solution: A centrifugal filtration device for the preparation of hydrolyzed keratin, including a body. A centrifugal tank is provided on the body. An organic top cover is provided above the centrifugal tank. A driving motor is installed on the top cover. The output end of the driving motor is connected to a mounting plate. Centrifugal mechanisms capable of self-filtering are provided at both ends of the mounting plate. A discharging mechanism is provided at the bottom of the centrifugal tank.

[0005] Preferably, the centrifugal mechanism includes a side frame installed on the side wall of the mounting plate. A connecting ring is provided inside the side frame. A centrifugal tube is installed inside the connecting ring. A filter tube is provided inside the centrifugal tube. An inner tube is movably inserted inside the filter tube. The bottom of the inner tube is connected to a bottom ring. Two mounting blocks are provided on both sides of the bottom ring. An inner cavity is provided inside the mounting block. A self-locking mechanism is provided inside the inner cavity.

[0006] Preferably, the end of the side frame is of a "C" - shaped structure. The connecting ring is rotatably installed at the end of the side frame. A card slot is provided inside the connecting ring. A clamping column is provided on the side wall of the centrifugal tube. The clamping column is clamped in the card slot.

[0007] Preferably, filter holes are provided on the side wall of the filter tube.

[0008] Preferably, the discharging mechanism includes a first telescopic rod, the top of the first telescopic rod is connected to a center disk, support arms are connected on both sides of the center disk, the ends of the support arms are connected to a fixed sleeve, a movable ring is movably inserted in the fixed sleeve, a docking joint is provided in the middle of the movable ring, a base frame is connected to the bottom of the fixed sleeve, a second telescopic rod is installed in the base frame, the top of the second telescopic rod is connected to a pump, the pump and the bottom of the movable ring are fixedly connected, and the water inlet end of the pump is connected to the docking joint, the water outlet end of the pump is connected to a drainage hose, and the drainage hose is connected to the drainage port on the outer wall of the body.

[0009] Preferably, the movable ring electromagnet.

[0010] Preferably, the self-locking mechanism includes a gear shaft rotatably installed inside the inner cavity, and a first movable plate and a second movable plate are slidably arranged inside the inner cavity, the first movable plate and the second movable plate are vertically arranged, and a first tooth plate and a second tooth plate are respectively provided on the side walls of the first movable plate and the second movable plate, and the first tooth plate and the second movable plate are both meshed with the gear shaft, and a positioning hole is opened on the side wall of the bottom ring, one end of the first movable plate is inserted into the positioning hole, and the bottom end of the second movable plate passes through the bottom surface of the mounting block, and the top of the second movable plate is connected to a push spring.

[0011] Preferably, a sliding track is provided inside the inner cavity, and the first movable plate and the second movable plate slide in the corresponding sliding track.

[0012] A preparation process for hydrolyzed keratin comprises the following steps:

[0013] S1: Pretreatment: The wool containing keratin is washed and dried, and then soaked in a 0.2% sodium hydroxide solution at 50°C for 1 hour. The wool soaked in the solution is then washed to remove the residual alkali solution, and then dried and crushed into wool powder using a grinder;

[0014] S2: Oxidation treatment: add 5%-10% hydrogen peroxide to 100 kg of raw material powder treated in the previous step at room temperature, adjust the solution pH to 7-12, quickly heat to 60-100°C, keep warm for 0.5-3 hours, then cool to 30-40°C, and filter to obtain a pretreated solution;

[0015] S3: Enzymatic hydrolysis: adjust the pH of the pre-treated solution in the previous step to 8-11, add 0.1%-10% alkaline protease and treat at 70-90°C for 4-8 hours to obtain a preliminary wool keratin hydrolyzate;

[0016] S4: Purification: After the enzymatic hydrolysis is completed, the temperature is raised to 95°C for 30-40 minutes to inactivate the enzyme. After the enzyme inactivation is completed, activated carbon decolorization is performed, and finally centrifugation or filtration is performed to obtain a clear solution;

[0017] S5: The collected supernatant is ultrafiltered through an ultrafilter with a molecular weight of 500 to 3000 Da, and the ultrafiltration filtrate and ultrafiltration retentate are collected respectively;

[0018] S6: The filtrate is concentrated until the solid content reaches 30% or more, thereby obtaining a hydrolyzed keratin concentrate.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. The present invention proposes a process for preparing hydrolyzed keratin and a centrifugal filtration device thereof. The production process of this process is safe, simple, convenient, and economical, and is amenable to industrial large-scale production. The hydrolyzed keratin produced by this process has a relatively small molecular weight range of approximately 500-3000, and the molecular weight is relatively low. The hydrolyzed keratin produced by this process can be regulated in terms of solid content, pH value, and relative molecular weight. The device can simultaneously perform centrifugal filtration during preparation, making operation convenient and improving production efficiency.

[0021] 2. When the wool keratin hydrolyzate is centrifugally filtered, the hydrolyzate is first placed in the centrifuge tube 8, and then the centrifuge tube is mounted on the connecting ring. During installation, the corresponding card column is connected to the card slot, and then the drive motor is started and aligned to perform the centrifugal operation. After the centrifugation is completed, the wool keratin hydrolyzate inside will produce stratification, and it is necessary to filter it to take out the upper clear liquid. Because a self-filtering centrifuge mechanism is provided, the filtering and liquid collection operation can be performed automatically, which is more convenient.

[0022] 3. When filtering and taking liquid, adjust the position of the centrifuge tube so that it is above the fixed sleeve, control the extension of the first telescopic rod to push up the center disk and the connected structure, then the movable ring moves up and is adsorbed together with the bottom ring, and the joint and the bottom of the inner tube are connected to each other, and then the pump is started to absorb the upper clear liquid filtered by the filter tube inside the centrifuge tube through the top of the inner tube, and then discharged and collected through the drain hose and the drain port, while the second telescopic rod is contracted to pull down the pump, so that the movable ring pulls the push spring down, and the upper clear liquid can be absorbed. There is no need to take out the centrifuge tube for additional operations, which is more convenient.

[0023] 4. Due to the self-locking structure, the first movable plate is inserted into the positioning hole to clamp the bottom ring, and the inner tube will not drop. The opening on the top of the inner tube is located above the hydrolyzed liquid to prevent leakage. When the fixed sleeve is above, the fixed sleeve pushes the second movable plate up, and the second toothed plate drives the gear shaft to rotate and then drives the first movable plate to move through the first toothed plate. The end of the first movable plate is removed from the positioning hole. When the movable ring and the bottom ring are sucked together, the inner tube can be pulled down. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the appearance structure of the device of the present invention.

[0025] Figure 2 It is a schematic diagram of the internal structure of the present invention.

[0026] Figure 3 It is a schematic diagram of the internal structure of the centrifugal tank of the present invention.

[0027] Figure 4 It is a schematic diagram of the connecting ring structure of the present invention.

[0028] Figure 5 It is a schematic diagram of the centrifuge tube structure of the present invention.

[0029] Figure 6 This is a cross-sectional view of the centrifuge tube structure of the present invention.

[0030] Figure 7 for Figure 6 Enlarged view of point A in the middle.

[0031] Figure 8 It is a structural schematic diagram of the fixing sleeve of the present invention.

[0032] In the figure: body 1, centrifugal tank 2, top cover 3, drive motor 4, mounting plate 5, side frame 6, connecting ring 7, slot 701, centrifugal tube 8, clamping column 801, filter tube 9, inner tube 10, bottom ring 11, positioning hole 12, mounting block 13, inner cavity 14, gear shaft 15, first movable plate 16, first tooth plate 17, second movable plate 18, second tooth plate 19, push spring 20, first telescopic rod 21, center plate 22, support arm 23, fixed sleeve 24, movable ring 25, docking joint 26, pump 27, base frame 28, second telescopic rod 29, drain hose 30, drain port 31. DETAILED DESCRIPTION

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0034] See also Figures 1 to 8, the present invention provides a technical solution: a centrifugal filtration device for the preparation of hydrolyzed keratin, including a body 1, on which a centrifugal tank 2 is provided, above the centrifugal tank 2 there is a machine top cover 3, on the machine top cover 3 a driving motor 4 is installed, the output end of the driving motor 4 is connected to a mounting disc 5, at both ends of the mounting disc 5 there are centrifugal mechanisms that can self-filter. The centrifugal mechanism includes a side frame 6 installed on the side wall of the mounting disc 5, inside the side frame 6 there is a connecting ring 7, inside the connecting ring 7 a centrifugal tube 8 is installed. The end of the side frame 6 is of a "C" shape structure, the connecting ring 7 is rotatably installed at the end of the side frame 6, a card slot 701 is opened inside the connecting ring 7, on the side wall of the centrifugal tube 8 there is a clamping column 801, and the clamping column 801 is clamped in the card slot 701. During installation, align the clamping column 801 and insert it into the card slot, then rotate the centrifugal tube 8 to complete the installation.

[0035] Inside the centrifugal tube 8 there is a filter tube 9, on the side wall of the filter tube 9 there are filter holes, inside the filter tube 9 there is an inner tube 10 inserted movably. At the top of the inner tube 10 there is a liquid inlet. Before the solution in the centrifugal tube 8 enters the liquid inlet, it needs to be filtered by the filter tube 9 once. The bottom of the inner tube 10 is connected to a bottom ring 11, on both sides of the bottom ring 11 there are two mounting blocks 13, inside the mounting block 13 there is an inner cavity 14, and inside the inner cavity 14 there is a self-locking mechanism. The self-locking mechanism includes a gear shaft 15 rotatably installed inside the inner cavity 14. Inside the inner cavity 14 there is a first movable plate 16 and a second movable plate 18 slidingly arranged. Inside the inner cavity 14 there are sliding tracks, and the first movable plate 16 and the second movable plate 18 slide in the corresponding sliding tracks, which can make the movement of the first movable plate 16 and the second movable plate 18 more smooth and stable. The first movable plate 16 and the second movable plate 18 are vertically arranged. On the side walls of the first movable plate 16 and the second movable plate 18 there are respectively a first toothed plate 17 and a second toothed plate 19, both the first toothed plate 17 and the second movable plate 18 are meshed with the gear shaft 15. On the side wall of the bottom ring 11 there is a positioning hole 12, one end of the first movable plate 16 is inserted into the positioning hole 12, the bottom end of the second movable plate 18 passes through the bottom surface of the mounting block 13, and the top end of the second movable plate 18 is connected to a top push spring 20.

[0036] A discharging mechanism is provided at the bottom of the centrifugal trough 2, which includes a first telescopic rod 21, the top of the first telescopic rod 21 is connected to a center disk 22, support arms 23 are connected on both sides of the center disk 22, the ends of the support arms 23 are connected to a fixed sleeve 24, a movable ring 25 is movably inserted in the fixed sleeve 24, the movable ring 25 is an electromagnet, which can absorb the iron bottom ring 11 when energized, a docking joint 26 is provided in the middle of the movable ring 25, a base frame 28 is connected to the bottom of the fixed sleeve 24, a second telescopic rod 29 is installed in the base frame 28, a pump 27 is connected to the top of the second telescopic rod 29, the pump 27 is fixedly connected to the bottom of the movable ring 25, and the water inlet end of the pump 27 is connected to the docking joint 26, the water outlet end of the pump 27 is connected to a drainage hose 30, and the drainage hose 30 is connected to the drainage port 31 on the outer wall of the body 1.

[0037] Because of the self-locking structure, the first movable plate 16 is inserted into the positioning hole 12 to clamp the bottom ring 11, and the inner tube 10 will not drop. The opening at the top of the inner tube 10 is located above the hydrolyzed liquid to prevent leakage. When it is above the fixed sleeve 24, the fixed sleeve 24 pushes the second movable plate 18 up, and the second tooth plate 19 drives the gear shaft 15 to rotate and then drives the first movable plate 16 to move through the first tooth plate 17. The end of the first movable plate 16 is removed from the positioning hole 12. When the movable ring 25 and the bottom ring 11 are sucked together, the inner tube 10 can be pulled down.

[0038] A preparation process for hydrolyzed keratin comprises the following steps:

[0039] S1: Pretreatment: The wool containing keratin is washed and dried, and then soaked in a 0.2% sodium hydroxide solution at 50°C for 1 hour. The wool soaked in the solution is then washed to remove the residual alkali solution, and then dried and crushed into wool powder using a grinder;

[0040] S2: Oxidation treatment: add 5%-10% hydrogen peroxide to 100 kg of raw material powder treated in the previous step at room temperature, adjust the solution pH to 7-12, quickly heat to 60-100°C, keep warm for 0.5-3 hours, then cool to 30-40°C, and filter to obtain a pretreated solution;

[0041] S3: Enzymatic hydrolysis: adjust the pH of the pre-treated solution in the previous step to 8-11, add 0.1%-10% alkaline protease and treat at 70-90°C for 4-8 hours to obtain a preliminary wool keratin hydrolyzate;

[0042] S4: Purification: After the enzymatic hydrolysis is completed, the temperature is raised to 95°C for 30-40 minutes to inactivate the enzyme. After the enzyme inactivation is completed, activated carbon decolorization is performed, and finally centrifugation or filtration is performed to obtain a clear solution;

[0043] S5: The collected supernatant is ultrafiltered through an ultrafilter with a molecular weight of 500 to 3000 Da, and the ultrafiltration filtrate and ultrafiltration retentate are collected respectively;

[0044] S6: The filtrate is concentrated until the solid content reaches 30% or more, thereby obtaining a hydrolyzed keratin concentrate.

[0045] The preparation process of hydrolyzed keratin and the centrifugal filtration device proposed by the present invention are safe, simple, convenient, economical, and easy to industrialize and mass-produce. The hydrolyzed keratin produced by the process has a relatively small molecular weight range of about 500-3000, and the molecular weight is relatively small. The hydrolyzed keratin produced by the process can be regulated in terms of solid content, pH value, and relative molecular weight. The device can simultaneously perform centrifugal filtration during preparation, is easy to operate, and improves production efficiency.

[0046] During actual use, the hydrolyzate is first placed in the centrifuge tube 8, and then the centrifuge tube 8 is mounted on the connecting ring 7. During installation, the clamping column 801 is correspondingly connected to the clamping groove 701, and then the drive motor 4 is started to align and perform the centrifugal operation. After the centrifugation is completed, the wool keratin hydrolyzate inside will produce stratification, and it is necessary to filter it to take out the upper clear liquid. Because a self-filtering centrifuge mechanism is provided, the operation of filtering and taking out the liquid can be performed automatically, which is more convenient. When filtering and taking liquid, adjust the position of the centrifuge tube 8 so that it is above the fixed sleeve 24, control the first telescopic rod 21 to extend to push up the center disk 22 and the structure connected to it, at this time the movable ring 25 moves up and is adsorbed together with the bottom ring 11, and the docking joint 26 is connected to the bottom of the inner tube 10, and then the pump 27 is started to absorb the upper clear liquid filtered by the filter tube 9 inside the centrifuge tube 8 through the top of the inner tube 10, and then discharged and collected through the drain hose 30 and the drain port 31, and at the same time the second telescopic rod 29 contracts to pull the pump 27 down, so that the movable ring 25 pulls the push spring 20 down, and the upper clear liquid can be absorbed, and there is no need to take out the centrifuge tube 8 for additional operation, which is more convenient.

[0047] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. Preparation of hydrolyzed keratin A centrifugal filter device comprising an organism (1), characterized in that: A centrifugal groove (2) is provided on the body (1). An engine top cover (3) is provided above the centrifugal groove (2). A drive motor (4) is installed on the engine top cover (3). The output end of the drive motor (4) is connected to a mounting disc (5). Centrifugal mechanisms that can self-filter are provided at both ends of the mounting disc (5). The centrifugal mechanisms include side frames (6) installed on the side walls of the mounting disc (5). A connecting ring (7) is provided inside the side frame (6). A centrifuge tube (8) is installed inside the connecting ring (7). A filter tube (9) is provided inside the centrifuge tube (8). An inner tube (10) is movably inserted into the filter tube (9). The bottom of the inner tube (10) is connected to a bottom ring (11). Two mounting blocks (13) are provided on both sides of the bottom ring (11). An inner cavity (14) is provided inside the mounting block (13). A self-locking mechanism is provided inside the inner cavity (14). The self-locking mechanism includes a gear shaft (15) rotatably installed inside the inner cavity (14). A first movable plate (16) and a second movable plate (18) are slidably provided inside the inner cavity (14). The first movable plate (16) and the second movable plate (18) are perpendicularly arranged. First tooth plates (17) and second tooth plates (19) are respectively provided on the side walls of the first movable plate (16) and the second movable plate (18). The first tooth plate (17) and the second movable plate (18) are both meshed and connected to the gear shaft (15). A positioning hole (12) is opened on the side wall of the bottom ring (11). One end of the first movable plate (16) is inserted into the positioning hole (12). The bottom end of the second movable plate (18) passes through the bottom surface of the mounting block (13). The top end of the second movable plate (18) is connected to a top push spring (20). A discharge mechanism is provided at the bottom of the centrifugal groove (2). The discharge mechanism includes a first telescopic rod (21). The top end of the first telescopic rod (21) is connected to a central disc (22). Support arms (23) are connected to both sides of the central disc (22). The end of the support arm (23) is connected to a fixed sleeve (24). A movable ring (25) is movably inserted into the fixed sleeve (24). A docking head (26) is provided in the middle of the movable ring (25). A bottom frame (28) is connected to the bottom of the fixed sleeve (24). A second telescopic rod (29) is installed in the bottom frame (28). The top end of the second telescopic rod (29) is connected to a pump (27). The pump (27) and the bottom of the movable ring (25) are fixedly connected. The water inlet end of the pump (27) is connected to the docking head (26). The water outlet end of the pump (27) is connected to a liquid discharge hose (30). The liquid discharge hose (30) is connected to a liquid discharge port (31) on the outer wall of the body (1).

2. The centrifugal filter device for preparing hydrolyzed keratin according to claim 1, characterized in that: The end of the side frame (6) is of a "C" type structure. The connecting ring (7) is rotatably installed at the end of the side frame (6). A card slot (701) is opened inside the connecting ring (7). A clamping column (801) is provided on the side wall of the centrifuge tube (8). The clamping column (801) is clamped in the card slot (701).

3. The centrifugal filter device for preparing hydrolyzed keratin according to claim 1, characterized in that: Filter holes are provided on the side wall of the filter tube (9).

4. The centrifugal filter device for preparing hydrolyzed keratin according to claim 1, characterized in that: The movable ring (25) is an electromagnet.

5. The centrifugal filter device for preparing hydrolyzed keratin according to claim 1, characterized in that: A sliding track is provided inside the inner cavity (14), and the first movable plate (16) and the second movable plate (18) slide in the corresponding sliding tracks.

6. A preparation process for the centrifugal filter device for preparing hydrolyzed keratin according to any one of claims 1 to 5, characterized in that: The following steps are included: S1: Pretreatment: The wool containing keratin is washed and dried, and then soaked in a 0.2% sodium hydroxide solution at 50°C for 1 hour. The wool soaked in the solution is then washed to remove the residual alkali solution, and then dried and crushed into wool powder using a grinder; S2: Oxidation treatment: add 5%-10% hydrogen peroxide to 100 kg of raw material powder treated in the previous step at room temperature, adjust the solution pH to 7-12, quickly heat to 60-100°C, keep warm for 0.5-3 hours, then cool to 30-40°C, and filter to obtain a pretreated solution; S3: Enzymatic hydrolysis: adjust the pH of the pre-treated solution in the previous step to 8-11, add 0.1%-10% alkaline protease and treat at 70-90°C for 4-8 hours to obtain a preliminary wool keratin hydrolyzate; S4: Purification: After the enzymatic hydrolysis is completed, the temperature is raised to 95°C for 30-40 minutes to inactivate the enzyme. After the enzyme inactivation is completed, activated carbon decolorization is performed, and finally centrifugation or filtration is performed to obtain a clear solution; S5: The collected supernatant is ultrafiltered through an ultrafilter with a molecular weight of 500 to 3000 Da, and the ultrafiltration filtrate and ultrafiltration retentate are collected respectively; S6: The filtrate is concentrated until the solid content reaches 30% or more, thereby obtaining a hydrolyzed keratin concentrate.

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