Method for extracting protein multi-level structure by periodate oxidation

The use of potassium iodate for mild oxidation at room temperature allows for the precise extraction of multi-level protein structures, addressing the challenges of existing methods by preserving protein integrity and enabling the production of high-performance materials.

CN118598974BActive Publication Date: 2025-03-18HUAZHONG AGRI UNIV
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Patent Information

Application Number
CN202410934204.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-07-12
Publication Date
2025-03-18
Estimated Expiration
2044-07-12

AI Technical Summary

Technical Problem

Current methods for extracting proteins from biological materials, such as keratin-based structures, often damage the protein structures due to harsh conditions, leading to incomplete or unstable protein recovery, particularly for complex multi-level structures like keratin, which are challenging to dissolve and process without breaking chemical bonds.

Method used

A method using mild and selective oxidation with potassium iodate (KIO3) at room temperature under light protection to oxidize proteins, controlling reaction time to achieve precise extraction of multi-level protein structures, avoiding the destruction of protein bonds and maintaining structural integrity.

Benefits of technology

This method effectively preserves the natural micro- and nano-structure of proteins, enabling the production of high-performance protein-based functional materials with improved efficiency, control, and environmental friendliness, suitable for various protein types including keratin, collagen, and others.

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Abstract

The present invention discloses a method for extracting a multi-level structure of a protein by periodate oxidation, and relates to the field of protein separation and extraction. The steps of the method include: mixing a bio-based raw material and periodic acid; adjusting the pH of the reaction solution with an alkali solution; performing an oxidation reaction at room temperature in the dark; and dialyzing, filtering, settling, and centrifuging the reaction solution to obtain a multi-level structure protein product. The method provided by the present invention does not require complex pretreatment and defatting treatment of the bio-based raw material, has mild reaction conditions, and is simple to operate. By regulating the oxidation reaction time, different levels of protein biological structures can be obtained, and the method is highly efficient, controllable, and environmentally friendly.
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