Multi-throughput fully automatic nucleic acid detection all-in-one machine and operation steps

Abstract

The present invention relates to the technical field of detection equipment, specifically to a multi-throughput fully automatic nucleic acid detection integrated machine and operating steps, including a detection host and a horizontally movable arm arranged inside the detection host and capable of horizontal movement and positioning, a lifting main arm and a lifting auxiliary arm are arranged below the horizontally movable arm, the lifting main arm and the lifting auxiliary arm are both capable of lifting and lowering, and a frame plate is fixedly arranged on the lower surface of the lifting main arm; when the multi-throughput fully automatic nucleic acid detection integrated machine of the present invention samples a sample tube, the horizontally movable arm only needs to perform one positioning movement, and the process of unscrewing and screwing on the sample tube cap is omitted, so that the overall sampling speed is greatly improved, and the detection speed can be improved in emergency large-scale detection situations.

Description

Technical Field

[0001] The present invention relates to the technical field of detection equipment, and specifically to a multi-throughput fully automatic nucleic acid detection all-in-one machine and operating steps. Background Art

[0002] The nucleic acid detection all-in-one machine is an integrated and automated device designed to complete the entire process of nucleic acid detection efficiently and conveniently. It integrates multiple links such as sample collection, processing, nucleic acid extraction, amplification and detection into one device, reducing the tedious steps of transferring samples between different instruments and reducing the risk of cross-contamination. Regarding the sample collection structure, the nucleic acid detection all-in-one machine in the prior art usually integrates two modules on the horizontal movable arm, one module is a mechanical claw for unscrewing the cap of the sample tube, and the other module is a lifting needle tube for sampling. When working, the mechanical claw will first be positioned with the sample tube, and then the cap will be unscrewed. Then, another lifting needle tube module will be moved over and positioned with the sample tube. After sampling is completed, it needs to be positioned again and the cap of the sample tube will be screwed on by the mechanical claw. The purpose of screwing on the cap of the sample tube is to retain the sample so that it can be retested in the event of a positive test or other circumstances. During this period, the horizontal movable arm needs to be controlled and positioned multiple times, which increases the entire action time. Unscrewing and screwing on the cap of the sample tube also requires a long delay, resulting in a long working time for the entire sampling system. In the case of a more urgent epidemic, the detection speed is too low. Summary of the Invention

[0003] The purpose of the present invention is to provide a multi-throughput fully automatic nucleic acid detection all-in-one machine and operating steps to solve the problems raised in the above background technology.

[0004] To achieve the above-mentioned objectives, the present invention provides the following technical solutions: a multi-throughput fully automatic nucleic acid detection integrated machine, comprising a detection host and a horizontally movable arm arranged inside the detection host and capable of horizontal movement and positioning, a lifting main arm and a lifting auxiliary arm are arranged below the horizontally movable arm, the lifting main arm and the lifting auxiliary arm are both capable of lifting and lowering, a frame plate is fixedly provided on the lower surface of the lifting main arm, a cover-breaking thorn tube is fixedly provided on the lower surface of the frame plate, a sampling needle tube is provided on the lifting auxiliary arm, the sampling needle tube is coaxial with the cover-breaking thorn tube, the inner diameter of the pipe of the cover-breaking thorn tube is larger than the outer diameter of the pipe of the sampling needle tube, a sealing swing arm is swingably installed on one side of the frame plate, a glue coating control module is provided on the frame plate, and the glue coating control module cooperates with the sealing swing arm to seal the perforation left by the cover-breaking thorn tube.

[0005] The glue coating control module includes a platform support plate, a swing arm drive box and a swing arm shaft. The platform support plate is fixedly installed with the frame plate body. The swing arm drive box is fixedly provided with a swing arm drive box. The swing arm drive box is provided with a swing arm shaft. The other end of the swing arm shaft is fixedly installed with the sealing hole swing arm. The swing arm drive box drives the sealing hole swing arm to swing through the swing arm shaft. A glue ball conveying cavity is opened in the sealing hole swing arm. When the sealing hole swing arm swings to the bottom, the lower end opening of the glue ball conveying cavity is coaxial with the cover-breaking puncture tube.

[0006] A fixed ball tube is embedded and fixed on the platform support plate, and a storage box is connected to the upper end of the fixed ball tube. Both the fixed ball tube and the storage box are provided with hole-blocking balls. A ball rotating baffle and a matching baffle are inserted through the side wall of the fixed ball tube. The matching baffle is located above the ball rotating baffle. A backrest plate is fixedly provided on the surface of the fixed ball tube, and the backrest plate limits the matching baffle.

[0007] A lower rack is provided on the upper surface of the ball rotating baffle, and an upper rack is provided on the lower surface of the mating baffle. A switching gear is meshed between the lower rack and the upper rack. A baffle control cylinder is provided at the end of the ball rotating baffle away from the fixed ball tube, and the baffle control cylinder is used to drive the ball rotating baffle to move along the length direction of the ball rotating baffle.

[0008] A friction rotor is embedded in the upper surface of the ball rotating baffle, a transmission cavity is opened inside the ball rotating baffle, a first pulley and a second pulley are arranged in the transmission cavity, the first pulley is coaxially connected and fixed to the friction rotor, a transmission belt is sleeved on the outside of the first pulley and the second pulley, a baffle motor is fixedly arranged on the outside of the ball rotating baffle, and the baffle motor is connected to the second pulley for transmission.

[0009] A glue supply cavity is provided between the matching baffle and the rotating ball baffle, a vertical glue slit is provided on one side of the glue supply cavity, the glue supply cavity is connected with the interior of the fixed ball tube through the vertical glue slit, and a positive pressure glue delivery hole is provided on the outside of the glue supply cavity.

[0010] An intermediate fork groove is provided in the mating baffle, and a fixed spring piece is provided in the intermediate fork groove. The fixed spring piece protrudes from the end plane of the mating baffle. A side wall shift groove is provided through the side wall of the fixed ball tube, and a lifting shift piece is provided in the side wall shift groove. A shift cylinder is provided below the lifting shift piece, and the shift cylinder controls the lifting and lowering movement of the lifting shift piece. When the mating baffle moves toward the side wall shift groove, the fixed spring piece will be inserted into the side wall shift groove.

[0011] A limited moving block is provided on one side of the swing arm drive box, and an air hood cylinder is provided between the limited moving block and the swing arm drive box. The air hood cylinder is used to control the limited moving block to move along the axial direction of the swing arm shaft. An air hood is fixedly provided on the limited moving block, and an air hood connecting pipe is connected to the air hood. A swing arm plane is provided at the end of the sealing swing arm. When the air hood moves above the swing arm plane, the lower surface of the air hood will be in sealing contact with the swing arm plane.

[0012] The detection host is provided with a sample holder, the sample holder is provided with a sample tube, and the outside of the detection host is provided with a machine compartment sliding cover and a data display screen.

[0013] Operating steps of the multi-throughput fully automatic nucleic acid detection machine:

[0014] Step 1: The cap-breaking and puncturing tube is moved by the horizontally movable arm so that the cap-breaking and puncturing tube is moved above the sample tube to be sampled;

[0015] Step 2: Control the sealing swing arm to swing 90 degrees so that the sealing swing arm moves away from the bottom of the cap-breaking and piercing tube, and then control the cap-breaking and piercing tube and the sampling needle to descend simultaneously until the cap-breaking and piercing tube pierces the screw cap of the sample tube;

[0016] Step 3: After the cap-breaking puncture tube pierces the screw cap of the sample tube, the cap-breaking puncture tube stops descending, and the sampling needle tube continues to descend, so that the sampling needle tube passes through the interior of the cap-breaking puncture tube and extends into the sample tube to complete the sampling;

[0017] Step 4: The cap-breaking puncture tube and the sampling needle tube rise and reset, the sealing swing arm is controlled to rotate 90 degrees and reset, and the glue control module seals the perforation left by the cap-breaking puncture tube through the sealing swing arm.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The multi-throughput fully automatic nucleic acid detection all-in-one machine of the present invention is designed with a single module, compared with the two-module design in traditional technology. When sampling the sample tube, the horizontal moving arm only needs to perform one positioning movement, and the process of unscrewing and screwing on the sample tube cap is omitted, so that the overall sampling speed is greatly improved, and the detection speed can be improved in emergency large-scale detection situations; and the cap is pierced through the tube by breaking the cap to take the sample, and then immediately closed, which reduces the opening degree of the sample tube and reduces the probability of sample contamination.

[0020] The glue coating control module of the present invention cooperates with the sealing swing arm, which can efficiently seal the perforation left by the broken cap puncture tube on the screw cap of the sample tube; through the cooperation of the baffle and the rotating ball baffle, the hole-blocking ball in the fixed ball tube can be released individually; and through the cooperation of structures such as the friction rotor, the transmission belt and the glue coating vertical seam, the hole-blocking ball can be evenly coated with glue, ensuring that the hole-blocking ball can be adhered and sealed after moving to the perforation of the screw cap of the sample tube; and through the cooperation of structures such as the set fixed spring piece and the lifting paddle, the hole-blocking ball after glue coating can be bounced downward, ensuring that the hole-blocking ball after glue coating will be knocked into the glue ball conveying cavity, thereby improving the working stability of the module. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0022] Figure 2 It is a front view of the overall structure of the present invention.

[0023] Figure 3 for Figure 2 Enlarged schematic diagram of area A in the middle.

[0024] Figure 4 This is a partially cutaway schematic diagram of the detection host of the present invention.

[0025] Figure 5 for Figure 4 Enlarged schematic diagram of area B in the middle.

[0026] Figure 6 This is a schematic diagram of a partial cutaway view of the detection host of the present invention from another angle.

[0027] Figure 7 for Figure 6 Enlarged schematic diagram of area C in the middle.

[0028] Figure 8 for Figure 7 Enlarged schematic diagram of area D in the middle.

[0029] Figure 9 It is a three-dimensional half-section schematic diagram of the cap-breaking and tube-piercing portion of the present invention.

[0030] Figure 10 for Figure 9 Enlarged schematic diagram of area E in the middle.

[0031] Figure 11 for Figure 10 Enlarged schematic diagram of area F in the middle.

[0032] Figure 12 for Figure 10 Enlarged schematic diagram of the G area in the middle.

[0033] Figure 13 To match the baffle structure diagram.

[0034] Figure 14 Schematic diagram of the ball-turning baffle structure.

[0035] Figure: 1. Detection host; 2. Horizontal moving arm; 3. Main lifting arm; 4. Secondary lifting arm; 5. Frame plate; 6. Capping tube; 7. Sampling needle; 8. Sealing swing arm; 801. Platform support plate; 802. Swing arm drive box; 803. Swing arm shaft; 804. Rubber ball conveying chamber; 805. Fixed ball tube; 806. Storage box; 807. Rotating ball baffle; 808. Matching baffle; 809. Backrest plate; 810. Lower rack; 811. Upper rack; 812. Switching gear; 813. Baffle control cylinder; 814. Friction rotor; 815. Transmission chamber. 816. First pulley; 817. Second pulley; 818. Drive belt; 819. Baffle motor; 820. Glue supply chamber; 821. Vertical glue slit; 822. Positive pressure glue delivery hole; 823. Middle fork groove; 824. Fixed spring; 825. Side wall pulley groove; 826. Lifting pulley; 827. Puller cylinder; 828. Limiting moving block; 829. Air hood cylinder; 830. Air hood; 831. Air hood connecting pipe; 832. Swing arm plane; 833. Hole-blocking ball; 101. Sample holder; 102. Sample tube; 103. Cabin slide cover; 104. Data display screen. DETAILED DESCRIPTION

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0037] See also Figures 1 to 14 The present invention provides a technical solution: a multi-throughput fully automatic nucleic acid detection integrated machine, such as Figure 4 As shown in the figure, it includes a detection host 1 and a horizontally movable arm 2 that is arranged inside the detection host 1 and can be horizontally moved and positioned. The detection host 1 integrates processing, nucleic acid extraction, amplification and detection modules. The horizontally movable arm 2 is driven by a movable track coordinated by the X-axis and the Y-axis, so that the horizontally movable arm 2 can be moved and positioned in the horizontal plane. The above-mentioned movable track is a common mobile control device in the prior art, such as Figure 5As shown in the figure, a lifting main arm 3 and a lifting auxiliary arm 4 are provided below the horizontally movable arm 2. Both the lifting main arm 3 and the lifting auxiliary arm 4 can be lifted and lowered. A frame plate 5 is fixedly provided on the lower surface of the lifting main arm 3. A cover-breaking thorn tube 6 is fixedly provided on the lower surface of the frame plate 5. A sampling needle tube 7 is provided on the lifting auxiliary arm 4. The sampling needle tube 7 is coaxial with the cover-breaking thorn tube 6. The inner diameter of the pipe of the cover-breaking thorn tube 6 is larger than the outer diameter of the pipe of the sampling needle tube 7. A sealing swing arm 8 is swingably installed on one side of the frame plate 5. A glue coating control module is provided on the frame plate 5. The glue coating control module cooperates with the sealing swing arm 8 to seal the perforation left by the cover-breaking thorn tube 6.

[0038] like Figure 8 As shown in the figure, the glue coating control module includes a platform support plate 801, a swing arm drive box 802 and a swing arm shaft 803. The platform support plate 801 is fixedly installed with the frame plate 5. The swing arm drive box 802 is fixedly provided on the platform support plate 801. The swing arm drive box 802 is provided with a swing arm shaft 803. The other end of the swing arm shaft 803 is fixedly installed with the sealing hole swing arm 8. The swing arm drive box 802 drives the sealing hole swing arm 8 to swing through the swing arm shaft 803. A glue ball conveying chamber 804 is opened in the sealing hole swing arm 8; when the sealing hole swing arm 8 swings to the bottom, the lower end opening of the glue ball conveying chamber 804 is coaxial with the cover-breaking piercing tube 6.

[0039] A fixed ball tube 805 is embedded and fixed on the platform support plate 801, and a storage box 806 is connected to the upper end of the fixed ball tube 805. Both the fixed ball tube 805 and the storage box 806 are provided with a hole-blocking ball 833. A rotating ball baffle 807 and a matching baffle 808 are inserted into the side wall of the fixed ball tube 805. The matching baffle 808 is located above the rotating ball baffle 807. A backrest plate 809 is fixedly provided on the surface of the fixed ball tube 805, and the backrest plate 809 limits the matching baffle 808.

[0040] A lower rack 810 is provided on the upper surface of the ball rotating baffle 807, and an upper rack 811 is provided on the lower surface of the baffle 808. A switching gear 812 is meshed between the lower rack 810 and the upper rack 811. A baffle control cylinder 813 is provided at the end of the ball rotating baffle 807 away from the fixed ball tube 805. The baffle control cylinder 813 is used to drive the ball rotating baffle 807 to move along the length direction of the ball rotating baffle 807.

[0041] A friction rotor 814 is embedded in the upper surface of the ball rotating baffle 807, and a transmission cavity 815 is opened inside the ball rotating baffle 807. A first pulley 816 and a second pulley 817 are provided in the transmission cavity 815. The first pulley 816 is coaxially connected and fixed with the friction rotor 814. A transmission belt 818 is provided on the outside of the first pulley 816 and the second pulley 817. A baffle motor 819 is fixed on the outside of the ball rotating baffle 807, and the baffle motor 819 is connected to the second pulley 817 for transmission.

[0042] A glue supply cavity 820 is provided between the matching baffle 808 and the rotating ball baffle 807, and a vertical glue slit 821 is provided on one side of the glue supply cavity 820. The glue supply cavity 820 is connected to the interior of the fixed ball tube 805 through the vertical glue slit 821, and a positive pressure glue delivery hole 822 is provided on the outside of the glue supply cavity 820; when in use, the positive pressure glue delivery hole 822 is connected to the glue liquid through a gear pump, and the glue liquid is positively and quantitatively input into the glue supply cavity 820 through the gear pump; the glue liquid can be UV light-cured glue, which can only be cured under UV light irradiation; the UV light source can be set inside the detection host 1, but the sample and other detection agents and solutions need to be shielded from light.

[0043] An intermediate fork groove 823 is provided in the mating baffle 808, and a fixed spring piece 824 is provided in the intermediate fork groove 823. The fixed spring piece 824 protrudes from the end plane of the mating baffle 808. A side wall shift groove 825 is provided through the side wall of the fixed ball tube 805. A lifting shift piece 826 is provided in the side wall shift groove 825. A shift cylinder 827 is provided below the lifting shift piece 826. The shift cylinder 827 controls the lifting and lowering movement of the lifting shift piece 826. When the mating baffle 808 moves toward the side wall shift groove 825, the fixed spring piece 824 will be inserted into the side wall shift groove 825.

[0044] A limit moving block 828 is provided on one side of the swing arm drive box 802, and an air hood cylinder 829 is provided between the limit moving block 828 and the swing arm drive box 802. The air hood cylinder 829 is used to control the limit moving block 828 to move along the axial direction of the swing arm shaft 803. An air hood 830 is fixedly provided on the limit moving block 828, and an air hood connecting pipe 831 is connected to the air hood 830. A swing arm plane 832 is provided at the end of the sealing swing arm 8. When the air hood 830 moves above the swing arm plane 832, the lower surface of the air hood 830 will be in sealing contact with the swing arm plane 832.

[0045] A sample holder 101 is provided in the detection host 1, and a sample tube 102 is provided in the sample holder 101. A chamber sliding cover 103 and a data display screen 104 are provided on the outside of the detection host 1; the sample tube 102 and the sample holder 101 are both clamped and fixed in the detection host 1 to prevent the sample tube 102 from being lifted up when the cover-breaking puncture tube 6 rises.

[0046] Operating steps of the multi-throughput fully automatic nucleic acid detection machine:

[0047] Step 1: The cap-breaking puncture tube 6 is moved by the horizontally movable arm 2 so as to be positioned above the sample tube 102 to be sampled.

[0048] Step 2: Control the sealing swing arm 8 to swing 90 degrees so that the sealing swing arm 8 moves away from the bottom of the cap-breaking puncture tube 6, and then control the cap-breaking puncture tube 6 and the sampling needle tube 7 to descend simultaneously until the cap-breaking puncture tube 6 pierces the screw cap of the sample tube 102;

[0049] Step 3: After the cap-breaking puncture tube 6 pierces the screw cap of the sample tube 102, the cap-breaking puncture tube 6 stops descending, and the sampling needle 7 continues to descend, so that the sampling needle 7 passes through the interior of the cap-breaking puncture tube 6 and extends into the sample tube 102 to complete the sampling;

[0050] Step 4: The cap-breaking puncture tube 6 and the sampling needle tube 7 rise and reset, and the sealing swing arm 8 is controlled to rotate 90 degrees and reset. The glue coating control module seals the perforation left by the cap-breaking puncture tube 6 through the sealing swing arm 8.

[0051] The multi-flux fully automatic nucleic acid detection integrated machine of the present invention, when in use, Figure 1 As shown in FIG, the sample holder 101 and the sample tube 102 are placed inside the detection host 1. When the fully automatic nucleic acid detection all-in-one machine is working, the samples in the multiple sample tubes 102 are first sampled, as shown in FIG. Figure 4 and Figure 5 As shown in the figure, the horizontal movable arm 2 can drive the lifting main arm 3 and the lifting auxiliary arm 4 to move in a horizontal plane, and the cap-breaking and piercing tube 6 moves synchronously, so that the cap-breaking and piercing tube 6 corresponds to the sample tube 102 to be sampled up and down.

[0052] First, the swing arm driving box 802 controls the sealing swing arm 8 to rotate ninety degrees, so that the sealing swing arm 8 moves away from under the cap-breaking puncture tube 6, and then the lifting main arm 3 and the lifting auxiliary arm 4 descend synchronously, driving the cap-breaking puncture tube 6 and the sampling needle tube 7 to descend synchronously. The cap-breaking puncture tube 6 first pierces the screw cap of the sample tube 102. When the cap-breaking puncture tube 6 penetrates the screw cap of the sample tube 102, the lifting main arm 3 stops descending, so that the cap-breaking puncture tube 6 stops descending. At this time, the sampling needle tube 7 continues to move downward, and the sampling needle tube 7 will pass through the cap-breaking puncture tube 6 and extend into the sample tube 102 to complete the sampling.

[0053] After the sampling is completed, the capping puncture tube 6 and the sampling needle tube 7 rise and reset; then the sealing swing arm 8 is controlled to reverse 90 degrees and reset again, so that the sealing swing arm 8 is as shown in FIG. Figure 5 and Figure 10 As shown in FIG, the lower end opening of the rubber ball delivery cavity 804 is coaxial with the axis of the cap-breaking piercing tube 6.

[0054] like Figure 11As shown in FIG, the baffle motor 819 drives the second pulley 817 to rotate, which in turn rotates the first pulley 816 through the transmission belt 818. The first pulley 816 then rotates the friction rotor 814, which in turn rotates the plugging ball 833 through friction. At this time, positive pressure glue is fed through the positive pressure glue delivery port 822, and the glue overflows from the vertical glue slit 821. Combined with the rotation of the plugging ball 833, the surface of the plugging ball 833 above the friction rotor 814 is evenly coated with glue. The process described in this section can be completed in advance during the sampling process of the sampling needle 7.

[0055] like Figure 10 and Figure 11 As shown in the figure, the baffle control cylinder 813 drives the rotating ball baffle 807 to move in the direction of the baffle control cylinder 813, so that the rotating ball baffle 807 moves away from under the hole-blocking ball 833; at the same time, the lower rack 810 drives the switching gear 812 to rotate clockwise by meshing, and engages with the upper rack 811 to drive the matching baffle 808 to be inserted into the fixed ball tube 805. At this time, the matching baffle 808 separates and blocks the remaining hole-blocking balls 833, so that only the hole-blocking ball 833 above the friction rotating head 814 can fall; after the matching baffle 808 moves into place, the fixed spring piece 824 will be inserted into the side wall slot 825. At this time, the lifting paddle 826 is driven to rise by the paddle cylinder 827, so that the lifting paddle 826 paddles the fixed spring piece 824, and the fixed spring piece 824 will bounce downward to ensure that the glue-coated hole-blocking ball 833 is discharged into the glue ball conveying chamber 804.

[0056] like Figure 12 As shown in the figure, the air hood cylinder 829 drives the limit moving block 828 and the air hood 830 to move, so that the air hood 830 moves to the top of the swing arm plane 832. At this time, the air hood 830 is connected with the rubber ball conveying chamber 804, and the positive pressure airflow is input through the air hood connecting pipe 831. The plugging ball 833 coated with glue in the rubber ball conveying chamber 804 is driven by the air pressure to shoot downward and fall on the perforation of the screw cap of the sample tube 102. The perforation is sealed by the plugging ball 833 and the glue attached to the surface. At this time, it can be cured by UV lamp irradiation; by controlling the air pressure, the force of the plugging ball 833 spraying downward is controlled to ensure that the rebound force is within the viscosity range of the glue to avoid bouncing.

[0057] The fully automatic nucleic acid detection integrated machine of the present invention can achieve faster sampling and quickly seal and store the sample tubes 102. However, when some sample tubes 102 need to be re-tested, the caps of the sample tubes 102 to be re-tested must be replaced or manually tested separately.

[0058] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A multi-throughput fully automatic nucleic acid detection integrated machine, comprising a detection host (1) and a horizontally movable arm (2) arranged inside the detection host (1) and capable of horizontal movement and positioning, characterized in that: A lifting main arm (3) and a lifting auxiliary arm (4) are arranged below the horizontal movable arm (2). Both the lifting main arm (3) and the lifting auxiliary arm (4) are capable of lifting and lowering. A frame plate (5) is fixedly arranged on the lower surface of the lifting main arm (3). A cover-breaking piercing tube (6) is fixedly arranged on the lower surface of the frame plate (5). A sampling needle tube (7) is arranged on the lifting auxiliary arm (4). The sampling needle tube (7) is coaxially corresponding to the cover-breaking piercing tube (6). The inner diameter of the pipe of the cover-breaking piercing tube (6) is larger than the outer diameter of the pipe of the sampling needle tube (7). A sealing hole swing arm (8) is swingably installed on one side of the frame plate (5). A glue coating control device is arranged on the frame plate (5). The glue coating control module cooperates with the sealing hole swing arm (8) to seal the perforation left by the cap-breaking piercing tube (6); the glue coating control module comprises a platform support plate (801), a swing arm drive box (802) and a swing arm shaft (803); the platform support plate (801) is fixedly mounted on the frame plate body (5); a swing arm drive box (802) is fixedly arranged on the platform support plate (801); a swing arm shaft (803) is arranged on the swing arm drive box (802); the other end of the swing arm shaft (803) is fixedly mounted on the sealing hole swing arm (8); the swing arm drive box (802) drives the sealing hole swing arm (8) to swing via the swing arm shaft (803); the sealing hole swing arm (8) is provided with a rubber ball delivery chamber (804); when the sealing hole swing arm (8) swings to the lowest position, the lower end opening of the rubber ball delivery chamber (804) is coaxially corresponding to the cap-breaking piercing tube (6); a fixed ball tube (805) is embedded and fixed on the platform support plate (801), and a storage box (806) is provided at the upper end of the fixed ball tube (805), and a hole-blocking ball (833) is provided in both the fixed ball tube (805) and the storage box (806), and a rotating ball baffle (807) and a matching baffle (808) are interspersed on the side wall of the fixed ball tube (805), and the matching baffle (808) is located above the rotating ball baffle (807). 05) is fixedly provided with a backrest plate (809), and the backrest plate (809) limits the matching baffle plate (808); the upper surface of the ball-turning baffle plate (807) is provided with a lower rack (810), and the lower surface of the matching baffle plate (808) is provided with an upper rack (811), and a switching gear (812) is meshed between the lower rack (810) and the upper rack (811); the end of the ball-turning baffle plate (807) away from the fixed ball tube (805) is provided with a baffle control cylinder (813), and the baffle control cylinder (813) is used to drive the ball-turning baffle plate (807) to move along the length direction of the ball-turning baffle plate (807);A friction rotating head (814) is embedded in the upper surface of the rotating ball baffle (807), a transmission cavity (815) is provided inside the rotating ball baffle (807), a first pulley (816) and a second pulley (817) are provided in the transmission cavity (815), the first pulley (816) is coaxially connected and fixed to the friction rotating head (814), a transmission belt (818) is sleeved on the outside of the first pulley (816) and the second pulley (817), a baffle motor (819) is fixedly provided on the outside of the rotating ball baffle (807), the baffle motor (819) and the second pulley (817) are connected to each other. 17) transmission connection; a glue supply cavity (820) is provided between the matching baffle (808) and the rotating ball baffle (807); a glue supply cavity (820) is provided with a glue coating vertical slit (821) on one side of the glue supply cavity (820); the glue supply cavity (820) is connected to the inside of the fixed ball tube (805) through the glue coating vertical slit (821); the outside of the glue supply cavity (820) is connected with a positive pressure glue delivery hole (822); an intermediate fork groove (823) is provided in the matching baffle (808); a fixing spring sheet (824) is provided in the intermediate fork groove (823); the fixing spring sheet (824) protrudes out of the matching baffle (80 8), a side wall of the fixed ball tube (805) is penetrated by a side wall groove (825), a lifting paddle (826) is arranged in the side wall groove (825), a paddle cylinder (827) is arranged below the lifting paddle (826), the paddle cylinder (827) controls the lifting and lowering movement of the lifting paddle (826), when the matching baffle (808) moves toward the side wall groove (825), the fixed spring piece (824) will be inserted into the side wall groove (825); a limited moving block (828) is arranged on one side of the swing arm drive box (802), and the limited moving block (828 ) and the swing arm drive box (802), an air hood cylinder (829) is provided, the air hood cylinder (829) is used to control the limit moving block (828) to move along the axial direction of the swing arm shaft (803), an air supply hood (830) is fixedly provided on the limit moving block (828), an air supply hood connecting pipe (831) is connected to the air supply hood (830), and an end of the sealing swing arm (8) is provided with a swing arm plane (832), when the air supply hood (830) moves to above the swing arm plane (832), the lower surface of the air supply hood (830) will be in sealing contact with the swing arm plane (832). ; 2. The multi-throughput fully automatic nucleic acid detection integrated machine according to claim 1, characterized in that: The detection host (1) is provided with a sample holder (101), the sample holder (101) is provided with a sample tube (102), and the outside of the detection host (1) is provided with a chamber sliding cover (103) and a data display screen (104).

3. The operating steps of the multi-throughput fully automatic nucleic acid detection integrated machine according to claim 1 or 2 are characterized in that: Step 1: The cap-breaking piercing tube (6) is driven to move by the horizontally movable arm (2), so that the cap-breaking piercing tube (6) is moved above the sample tube (102) to be sampled; Step 2: Control the sealing swing arm (8) to swing 90 degrees so that the sealing swing arm (8) moves away from below the cap-breaking piercing tube (6), and then control the cap-breaking piercing tube (6) and the sampling needle tube (7) to descend simultaneously until the cap-breaking piercing tube (6) pierces the screw cap of the sample tube (102); Step 3: After the cap-breaking piercing tube (6) pierces the screw cap of the sample tube (102), the cap-breaking piercing tube (6) stops descending, and the sampling needle tube (7) continues to descend, so that the sampling needle tube (7) passes through the interior of the cap-breaking piercing tube (6) and extends into the sample tube (102) to complete the sampling; Step 4: the cap-breaking piercing tube (6) and the sampling needle tube (7) are raised and reset, the sealing swing arm (8) is controlled to rotate 90 degrees and reset, and the glue coating control module seals the perforation left by the cap-breaking piercing tube (6) through the sealing swing arm (8).

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