Vitamin B 6 Application in improving tomato's resistance to flooding stress

Abstract

The invention belongs to the technical field of crop planting, and specifically relates to the application of vitamin B6 in improving the ability of tomatoes to resist flooding stress. Spraying VB6 on leaves of tomato plants in the seedling stage can improve the ability of tomatoes to resist flooding, and can be used as a regulator for tomato seedlings to resist flooding environment, but VB6 has no effect on lettuce seedlings.

Description

Technical Field

[0001] The invention relates to the technical field of crop planting, and in particular to application of vitamin B6 in improving the ability of tomatoes to resist flooding stress. Background Art

[0002] With the change of global climate, waterlogging stress has become one of the main abiotic stresses. Waterlogging stress is a kind of abiotic stress. Heavy rainfall and flooding coupled with poor drainage often cause the soil to be flooded. Since seeds and seedlings are immersed in water, the plants are in a low-oxygen environment and the submerged soil also produces many harmful substances. These problems will cause the growth of plant seedlings to be damaged or even die, and finally lead to uneven emergence and poor seedling quality, seriously affecting the normal growth and development of plants.

[0003] Tomato is an annual or perennial herb of the genus Solanaceae. Tomatoes need more water, but do not need frequent and large-scale irrigation. Generally, soil moisture of 60-80% and air humidity of 45-50% are appropriate. If the air humidity is high, it will not only hinder normal pollination, but also cause serious diseases under high temperature and high humidity conditions, which will have a huge impact on the survival and yield of tomatoes, causing irreparable economic losses. At present, the main way to resist flooding stress is to improve the drainage system and the plant's own waterlogging resistance, but there is a lack of effective plant regulator pretreatment methods to resist flooding environments. Therefore, trying to quickly improve the waterlogging resistance of tomatoes under waterlogging stress has a certain positive effect on the growth and development of tomatoes. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides the use of vitamin B6 in improving the resistance of tomato seedlings to flooding stress.

[0005] Application of vitamin B6 in improving tomato's resistance to waterlogging stress.

[0006] Preferably, the vitamin B6 is used at a concentration of 1.0-2.5 mM. Specifically, water is used as a solvent to dissolve the vitamin B6, so that the vitamin B6 is used at a concentration of 1.0-2.5 mM.

[0007] Preferably, the method of improving the resistance of tomatoes to waterlogging stress is to improve the resistance of tomato roots, stems and leaves to waterlogging stress.

[0008] Preferably, the amount of vitamin B6 used is 1-3 mL / tomato plant.

[0009] A method for improving tomato's resistance to waterlogging stress, comprising applying the vitamin B6 to the tomato plants.

[0010] Preferably, the concentration of vitamin B6 is 1.0-2.5 mM.

[0011] Preferably, 1-3 mL of vitamin B6 is applied to each tomato plant.

[0012] Preferably, the application site is the leaves of tomato plants.

[0013] Preferably, the tomato plants are tomato plants in the seedling stage.

[0014] Preferably, the tomato variety is Moneymaker.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. 1.5mM VB6 pretreatment can improve the flooding tolerance of tomato seedlings; however, 1.5mM VB6 pretreatment cannot improve the survival rate of spinach and yellow mushroom seedlings; under flooding stress, 1.5mM VB6 pretreatment has no effect on lettuce seedlings.

[0017] 2. VB6 can be used as a regulator to help tomato seedlings resist flooding environments. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The concentration screening of VB6 to improve the flooding stress of tomato seedlings, wherein A: phenotype of tomato seedlings after 1-3 days of flooding after pretreatment with different concentrations of VB6; B: DAB staining of tomato leaves after 1-3 days of flooding after pretreatment with different concentrations of VB6;

[0019] Figure 2 The changes of H2O2 content in the leaves of tomato seedlings after 1-3 days of flooding after pretreatment with different concentrations of VB6;

[0020] Figure 3 The phenotypes of tomato seedlings after 1.5 mM VB6 pretreatment, 3 days of flooding, and 3 days of recovery;

[0021] Figure 4 The tomato leaves were pretreated with 1.5 mM VB6 and then DAB stained after being flooded for 3 days;

[0022] Figure 5 It is the statistics of daily changes in tomato plant height increased by 1.5mM VB6 pretreatment;

[0023] Figure 6 It is the statistics of survival rate of tomato seedlings after pretreatment with 1.5mM VB6;

[0024] Figure 7 The phenotypes of spinach seedlings after 1.5 mM VB6 pretreatment, 3 days of flooding, and 3 days of recovery;

[0025] Figure 8 The spinach leaves were pretreated with 1.5 mM VB6 and then DAB stained after being flooded for 3 days;

[0026] Fig. 9 This is the statistics of spinach seedling survival rate after 1.5mM VB6 pretreatment;

[0027] Fig.10 The phenotype of yellow gooseberry seedlings after 1.5mM VB6 pretreatment, 3 days of flooding and 3 days of recovery;

[0028] Fig.11 The leaves of the yellow gooseberry were DAB stained after 1.5 mM VB6 pretreatment and flooding for 3 days;

[0029] Fig.12 The survival rate of yellow mushroom seedlings was calculated after 1.5mM VB6 pretreatment;

[0030] Fig.13 The phenotype of lettuce seedlings after 1.5 mM VB6 pretreatment, 3 days of flooding and 3 days of recovery;

[0031] Fig.14 The DAB staining of lettuce leaves was performed after 1.5 mM VB6 pretreatment and flooding for 3 days;

[0032] Fig.15 The survival rate of lettuce seedlings was statistically analyzed after pretreatment with 1.5mM VB6. DETAILED DESCRIPTION

[0033] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention. The experimental methods described in the embodiments of the present invention are conventional methods unless otherwise specified.

[0034] Preparation of VB6 solution: Weigh the corresponding mass of VB6, use water as solvent, and dissolve the weighed VB6 in water to make the concentration of the VB6 solution be 1.0-2.5mM.

[0035] The tomato variety is called Moneymaker.

[0036] Example 1

[0037] A method for improving the flooding tolerance of tomatoes, comprising the following steps:

[0038] Sow a single tomato seed in a pot containing 1:1 nutrient soil and vermiculite; select 5-week-old tomato seedlings with strong growth and basically uniform growth, and start supplementing with VB6, that is, spray 1.0mM VB6 solution on the leaves of the seedlings, 50mL for every 32 plants, until water droplets form on the leaves.

[0039] Example 2

[0040] A method for improving the flooding tolerance of tomatoes, comprising the following steps:

[0041] Sow tomato seeds individually in a pot containing 1:1 nutrient soil and vermiculite; select 5-week-old tomato seedlings with strong growth and basically uniform growth, and start supplementing with VB6, that is, spray 1.5mM VB6 solution on the leaves of the seedlings, 50mL for every 32 plants, until water droplets form on the leaves.

[0042] Example 3

[0043] A method for improving the flooding tolerance of tomatoes, comprising the following steps:

[0044] Sow a single tomato seed in a pot containing 1:1 nutrient soil and vermiculite; select 5-week-old tomato seedlings with strong growth and basically uniform growth, and start supplementing with VB6, that is, spray 2.0mM VB6 solution on the leaves of the seedlings, 50mL for every 32 plants, until water droplets form on the leaves.

[0045] Example 4

[0046] A method for improving the flooding tolerance of tomatoes, comprising the following steps:

[0047] Sow tomato seeds individually in a pot containing 1:1 nutrient soil and vermiculite; select 5-week-old tomato seedlings with strong growth and basically uniform growth, and start supplementing with VB6, that is, spray 2.5mM VB6 solution on the leaves of the seedlings, 50mL for every 32 plants, until water droplets form on the leaves.

[0048] Comparative Example 1

[0049] The difference between Comparative Example 1 and Example 2 is that tomatoes are replaced with spinach, specifically:

[0050] Spinach seeds were sown individually in pots containing 1:1 nutrient soil and vermiculite; 5-week-old spinach seedlings with strong growth and basically uniform growth were selected, and VB6 supplementation was started, i.e., 1.5 mM VB6 solution was sprayed on the leaves of the seedlings, 50 mL was sprayed for every 32 plants, until water droplets formed on the leaves.

[0051] Comparative Example 2

[0052] The difference between Comparative Example 2 and Example 2 is that tomatoes are replaced with yellow mushrooms, specifically:

[0053] Sow a single yellow goji berry seed in a pot containing 1:1 nutrient soil and vermiculite; select 5-week-old yellow goji berry seedlings with strong growth and basically uniform growth, and start supplementing with VB6, that is, spray 1.5mM VB6 solution on the leaves of the seedlings, spray 50mL for every 32 plants, until water droplets form on the leaves.

[0054] Comparative Example 3

[0055] The difference between Comparative Example 3 and Example 2 is that tomatoes are replaced with lettuce, specifically:

[0056] Lettuce seeds were sown individually in pots containing 1:1 nutrient soil and vermiculite; 5-week-old lettuce seedlings with strong growth and basically uniform growth were selected, and VB6 was supplemented, that is, 1.5mM VB6 solution was sprayed on the leaves of the seedlings, 50mL was sprayed for every 32 plants, until water droplets formed on the leaves.

[0057] Verification test

[0058] 1. Set 5 spraying concentrations: 0.0mM VB6, 1.0mM VB6, 1.5mM VB6, 2.0mM VB6, 2.5mM VB6; 10 groups in total, 5 control groups, and 5 flooding treatment groups; after three consecutive days of supplementation, start the full flooding experiment of tomato plants to observe the phenotype of tomato plants, that is, whether the plants fall over and whether the leaves wilt; at the same time, DAB (diaminobenzidine) staining of tomato leaves: first, take 1g DAB and dissolve it in 2mL 0.5M hydrochloric acid (HCl), and adjust the pH to 5.8 with sodium hydroxide (NaOH) to prepare 1% DAB dye solution; secondly, completely immerse the leaves to be tested in the dye solution, vacuum the centrifuge tube and shake it every ten minutes, repeat three times until the leaves show water stains. Incubate overnight at room temperature; finally, wash with water 3-5 times, add 95% ethanol and immerse the leaves, place the centrifuge tube in an 80℃ water bath for decolorization, change the 95% ethanol every 10 minutes, and take out the sample after the green color of the sample has completely faded, take out the sample and take a photo to record the staining results.

[0059] Phenotypes of tomato plants after flooding Figure 1 As shown in A, as the flooding time increases, the tomato plants begin to fall over, which is most obvious on the third day and the leaves wilt. Figure 1 As shown in B, the DAB staining of tomato leaves became darker with the increase of flooding time, but tomato plants treated with different concentrations of VB6 showed different degrees of color change. Compared with the 0mM and 2.5mM VB6 treatments, the tomato seedlings treated with 1.0mM, 1.5Mm and 2.0Mm VB6 were lighter in color, indicating that the accumulated H2O2 content was less and the tomato seedlings were less damaged. Further, the H2O2 content in the leaves was detected, as shown in Figure 2 As shown, it is consistent with the DAB staining results, and 1.5mM VB6 has a better effect. Finally, 1.5mM VB6 is determined to be the optimal concentration.

[0060] 2. In order to further verify the beneficial effects of 1.5mM VB6, 1.5mM / L VB6 was sprayed on normally growing tomato seedlings. After three days of flooding, the plants resumed normal growth for three days, and the phenotypes of the tomatoes after flooding and recovery were compared. DAB staining experiments were performed on the leaves of the control and flooded tomatoes for three days to observe the color changes. Finally, the daily changes in plant height of tomatoes after flooding and recovery, as well as the survival rates of tomatoes after flooding and recovery were counted.

[0061] The results are as follows Figure 3 and Figure 4 , sprayed with 1.5mM / L VB6 pretreatment, the phenotypic state and DAB staining results of tomato seedlings after 3 days of recovery were better than those of the control 0.0mM; Figure 5 The results showed that the daily change of plant height of tomato seedlings pretreated with 1.5mM / L VB6 during the recovery period after flooding was maintained at 0.1-0.3cm, which was significantly higher than the 0.0-0.1cm of the control. Figure 6 The results showed that as the recovery time prolonged, the survival rate of tomato plants pretreated with 1.5mM / LVB6 remained at 44.4%-58.3%, significantly higher than 11.1%-13.9% of the control group, indicating that 1.5mM / LVB6 pretreatment can significantly improve the tomato seedlings' resistance to flooding.

[0062] 3. Finally, the seedlings of spinach, yellow mushroom and lettuce were used as materials, and 1.5mM / L VB6 was sprayed to conduct a flooding experiment. After three days of flooding, the plants resumed normal growth for three days, and the phenotypes of spinach, yellow mushroom and lettuce plants after flooding and recovery were observed; after three days of flooding, the leaves of spinach, yellow mushroom and lettuce were subjected to DAB staining experiments to observe the depth of leaf color and judge the damage of the leaves; finally, the survival rates of spinach, yellow mushroom and lettuce after flooding and recovery were counted.

[0063] The results show that Figure 7 , Figure 8 and Fig. 9 As shown, spinach seedlings pretreated with 1.5mM VB6 showed obvious wilting after three days of flooding recovery; compared with the control group, the spinach seedlings pretreated with 1.5mM VB6 after 3 days of flooding showed no obvious change in hydrogen peroxide staining color, all showing dark brown; after 5 days of recovery, the survival rate of spinach seedlings pretreated with 1.5mM VB6 was 10%, lower than the 30% of the control group.

[0064] like Fig.10 , Fig.11 and Fig.12As shown, the yellow gooseberry seedlings pretreated with 1.5mM VB6 showed obvious wilting after three days of flooding recovery; compared with the control group, the yellow gooseberry seedlings pretreated with 1.5mM VB6 for 3 days showed no obvious change in hydrogen peroxide staining color, all showing dark brown; after 5 days of recovery, the survival rate of the yellow gooseberry seedlings pretreated with 1.5mM VB6 was consistent with that of the control group, both being 0.

[0065] like Fig.13 , Fig.14 and Fig.15 As shown, the lettuce seedlings pretreated with 1.5mM VB6 showed no obvious wilting after three days of flooding recovery; compared with the control group, the hydrogen peroxide staining color of the lettuce seedlings pretreated with 1.5mM VB6 after 3 days of flooding showed no obvious change, and all were yellow; after 5 days of recovery, the survival rate of the lettuce seedlings pretreated with 1.5mM VB6 was consistent with that of the control group, both 100%.

[0066] In summary, under flooding stress, 1.5mM VB6 pretreatment could not improve the flooding resistance of spinach and yellow mushroom; at the same time, 1.5mM VB6 pretreatment had no effect on the growth of lettuce before and after flooding.

[0067] It should be noted that when the claims of the present invention involve numerical ranges, it should be understood that the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. In order to avoid redundancy, the present invention describes a preferred embodiment.

[0068] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0069] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. Application of vitamin B6 in improving tomato's resistance to waterlogging stress.

2. The use according to claim 1, characterized in that: The vitamin B6 is used at a concentration of 1.0-2.5 mM.

3. The use according to claim 2, characterized in that: The improving the ability of tomatoes to resist waterlogging stress is to improve the ability of tomato roots, stems and leaves to resist waterlogging stress.

4. The use according to claim 3, characterized in that: The usage of vitamin B6 is 1-3 mL / tomato plant.

5. A method for improving tomato resistance to flooding stress, characterized in that: Applying the vitamin B6 of claim 1 to tomato plants.

6. The method according to claim 5, characterized in that The concentration of vitamin B6 is 1.0-2.5 mM.

7. The method according to claim 6, characterized in that Apply 1-3 mL of vitamin B6 to each tomato plant.

8. The method according to claim 5, characterized in that The application site is the leaves of tomato plants.

9. The method according to claim 5, characterized in that The tomato plants are tomato plants in the seedling stage.

10. The method according to claim 5, characterized in that The tomato variety is Moneymaker.

Images