Seedling cultivation method of Nanyang Ying

Abstract

The invention discloses a method for raising seedlings of cyperus rotundus, which comprises first evenly sowing cyperus rotundus seeds after germination in a culture matrix, then injecting a rhizobium strain GZMP‑08 bacterial solution into the culture matrix, keeping the culture matrix moist, and inoculating and culturing for 7d to 10d; transplanting the bud seedlings after inoculation and culturing into a container bag containing a seedling culture matrix; managing light and water during the seedling stage; fertilizing when the seedlings are transplanted for 3 months; performing root cutting treatment when the seedlings are transplanted for 4 months; and the cyperus rotundus seedlings can be planted out when the seedlings are transplanted for 5 months. The method for raising seedlings of the invention can obtain fast-growing high-quality seedlings with neat seedling emergence, thick seedling diameter, and developed taproots, and is resource-saving, environmentally friendly, and has simple and easy processes, and is a sustainable development approach that breaks through the traditional method for raising seedlings of cyperus rotundus.

Description

Technical Field

[0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a high-efficiency seedling raising method for Orchidaceae. Background Art

[0002] For a long time, rapid growth and high yield in forestry have had to rely on chemical nitrogen fertilizers. However, the production of chemical fertilizers consumes a lot of energy, which will increase air pollution and greenhouse effect; the application of chemical fertilizers not only increases the cost of tree cultivation, but also leads to water and soil pollution, soil compaction, soil fertility decline, and destroys the ecological balance, which is the biggest obstacle to achieving sustainable development of forestry.

[0003] In nature, plants that can restore soil fertility are mainly nitrogen-fixing plants of the Leguminosae family. The symbiosis they form with rhizobia (Rh) can convert nitrogen in the atmosphere into ammonium and nitrates that can be directly used by plants. This biological nitrogen fixation process greatly reduces the dependence on chemical nitrogen fertilizers. The natural ability of the leguminous plant-rhizobium symbiosis is regarded as a key strategy to reduce the use of chemical fertilizers, reduce carbon footprints, enhance soil carbon and nitrogen storage capacity, and improve the adaptability of ecosystems to climate change. Therefore, exploring and utilizing the biological nitrogen fixation capacity of nitrogen-fixing tree species in forestry and establishing a sustainable, efficient and environmentally friendly modern forestry is an important topic in the current development of forestry and a strategic measure for the construction of ecological civilization.

[0004] Scholars at home and abroad generally believe that continued research in four areas, including improving host plants, screening excellent nitrogen-fixing strains, efficient inoculation methods, and planting management measures, will help to better leverage the nitrogen-fixing advantages of leguminous species and have far-reaching significance that cannot be matched by other forestry cultivation techniques and measures.

[0005] Falcatariafalcata is an evergreen tall tree of the genus Falcataria in the Fabaceae family. Its wood is light and tough, with a wood fiber mass fraction of up to 61.8%, making it an excellent raw material for artificial boards and papermaking. Its well-developed root system can form nodules, fix nitrogen, and improve soil. It can also be used in the construction of ecological forests and greening of areas around tropical and subtropical regions.

[0006] At present, the seedling cultivation method of Nanyang Ying is still based on the traditional high-dose fertilization (fertilization once every 15 days) and meticulous seedling management. It is unable to overcome the problems of uneven seedling emergence, thin seedling stems, and insufficient growth stamina during the seedling cultivation process. There are also few reports on methods of inoculating seedlings that fully utilize their own nitrogen-fixing advantages. Summary of the invention

[0007] Based on this, the purpose of the present invention is to provide a seedling raising method for Orchidaceae. The Orchidaceae seedlings cultivated by the seedling raising method of the present invention have uniform seedlings, thick seedling stems, and well-developed main roots. The process is simple, cost-saving and environmentally friendly.

[0008] The specific technical solutions for achieving the above-mentioned invention objectives include the following.

[0009] The present invention provides a method for raising seedlings of cycads, comprising the following steps:

[0010] (1) Evenly sowing the germinated Nanyang Ying seeds in the culture matrix, and then covering the Nanyang Ying seeds with the culture matrix, injecting the rhizobium strain GZMP-08 bacterial solution into the culture matrix, keeping the culture matrix moist, placing it under the conditions of temperature of 28° C. to 30° C., humidity of 70% to 80%, and natural light, and inoculating and culturing for 7 days to 10 days; the deposit number of the rhizobium strain GZMP-08 is CCTCC No: M 20232371;

[0011] (2) Transplanting the inoculated seedlings into a container bag containing a seedling medium; the seedling medium is loess, peat and vermiculite in a volume ratio of 7±0.1:2±0.1:1±0.1, or loess, peat and vermiculite in a volume ratio of 5±0.1:2±0.1:3±0.1;

[0012] (3) Light and water management during the seedling stage;

[0013] (4) Apply fertilizer 3 months after transplanting the seedlings; prune the roots 4 months after transplanting; and the Nanyangying seedlings can be planted out 5 months after transplanting.

[0014] The method for raising seedlings of the cycads of the present invention establishes an effective host-rhizobium symbiotic nitrogen fixation system by inoculating the cycads of the cycads after germination with the high-efficiency rhizobium GZMP-08, gives full play to the unique evolutionary advantage of the synergistic enhancement of the cycads of the cycads and the high-efficiency rhizobium, provides sufficient nutrition for the early growth of the cycads of the cycads, reduces the fertilization by 2 to 3 times, saves money and protects the environment; then the inoculated and cultured seedlings are transplanted into bags, and by exploring the formula of the seedling raising substrate and optimizing the placement of the seedling raising bags, fast-growing high-quality seedlings with uniform seedling emergence, thick seedling diameters and well-developed taproots are finally obtained.

[0015] The method for raising seedlings of the present invention reduces the number of fertilization times, simplifies maintenance procedures such as regular transplanting and grading, saves money and protects the environment, and simplifies the procedures. It is a sustainable development approach that breaks through the traditional method for raising seedlings of the present invention, and has great practical significance for promoting the sustainable development of forestry. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 These are pictures of the seedlings on the 1st day (A), 3rd day (B), and 7th day (C) after inoculation of rhizobia in Example 1 of the present invention.

[0017] Figure 2The figures are growth trends of 1-month-old (A), 3-month-old (B), and 5-month-old (C) seedlings in Example 1 of the present invention.

[0018] Figure 3 It is a comparison chart of the root nodule growth of 1-month-old (A), 3-month-old (B), and 5-month-old (C) seedlings of Example 1 of the present invention and Comparative Example 1.

[0019] Figure 4 This is a comparison chart of the seedling heights of 5-month-old seedlings in Test Example 2 of the present invention.

[0020] Figure 5 This is a comparison chart of the ground diameter of 5-month-old seedlings in Test Example 2 of the present invention.

[0021] Figure 6 This is a comparison chart of the average root length of 5-month-old seedlings in Test Example 2 of the present invention.

[0022] Figure 7 This is a comparison chart of the nodule weight of 5-month-old seedlings in Test Example 2 of the present invention.

[0023] Figure 8 This is a comparison chart of the above-ground fresh weight of 5-month-old seedlings in Test Example 2 of the present invention.

[0024] Fig. 9 This is a comparison chart of the fresh weight of roots of 5-month-old seedlings in Test Example 2 of the present invention.

[0025] Fig.10 This is a comparison chart of the above-ground dry weight of 5-month-old seedlings in Test Example 2 of the present invention.

[0026] Fig.11 This is a comparison chart of the dry weight of roots of 5-month-old seedlings in Test Example 2 of the present invention. DETAILED DESCRIPTION

[0027] In order to facilitate the understanding of the present invention, the present invention will be described more fully below. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0028] Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as those commonly understood by those skilled in the art to which the present invention belongs. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used in the present invention includes any and all combinations of one or more of the related listed items.

[0029] Unless otherwise specified, the examples are all based on conventional experimental conditions or the conditions recommended by the manufacturer's instructions. The raw materials and reagents used in the present invention are all commercially available, and any biological germplasm material can be provided to the outside for scientific research.

[0030] In the seedling raising method of the South China Sea jasmine of the present invention, the seeds after germination are first evenly sown in a culture matrix that has been sterilized at high temperature, and the cultured high-efficiency rhizobium strain GZMP-08 bacterial solution is injected into the culture matrix for 7-10 days of inoculation and cultivation, so that the South China Sea jasmine seedlings can be accurately, efficiently and at low cost inoculated with rhizobia, an effective host-rhizobium symbiotic nitrogen fixation system is established, sufficient nutrition is provided for the early growth of the South China Sea jasmine seedlings, fertilization is reduced by 2-3 times, and resources are saved and the environment is protected; then, the inoculated and cultivated seedlings are transplanted Compared with the traditional method of spot sowing when the seeds just emerge from the radicle (whitening) (uneven emergence of seedlings, unclear or no main root, and insufficient growth after afforestation), transplanting the seedlings into bags after inoculating rhizobia for 7 to 10 days can ensure that the seedlings emerge evenly, with obvious and stretched main roots. After a lot of exploration of the raw materials of the seedling medium and the volume ratio between the raw materials, it was found that only the loess heart soil with a volume ratio of 7±0.1:2±0.1:1±0.1 or 5±0.1:2±0.1:3±0.1 , peat soil, and vermiculite as the seedling medium can significantly promote the growth of the seedlings of the southern jacaranda and the nodulation of rhizobia (while the traditional seedling medium is loess + compound fertilizer, which is not conducive to the nodulation and nitrogen fixation of rhizobia); in addition, the present invention also optimizes the placement method of the seedling container bag (in the traditional seedling cultivation process of the southern jacaranda, it is generally necessary to transplant the seedlings 2 to 3 times to break the roots, that is, by moving each bagged seedling to prevent the roots from breaking through the seedling bag and piercing into the seedbed, which consumes a lot of man-hours, and the matrix in the seedling bag is easily loosened when transplanting the seedlings, affecting the development of the root system). Placing multiple seedling container bags in a seedling frame, and using objects with a volume similar to that of the seedling container bags to separate the rows of seedling container bags, can provide reasonable growth space and sufficient light for the seedlings, and promote the seedlings to be strong and neat; at the same time, it simplifies the procedure of transplanting and root cutting during the vigorous growth period of the seedlings, and only needs to move the seedling frame left and right to complete the root cutting process, the process is simple, and the original matrix in the seedling bag will not be damaged; and because the cultivated seedlings grow neatly, the work of grading and placing the seedlings before leaving the nursery is eliminated, simplifying the seedling management process. With the cooperation of the above key steps and key processes, the Nanyang Ying seedlings cultivated according to the method of the present invention have fully reached the specifications for leaving the nursery for afforestation at 5 months old, with an average seedling height of more than 30 cm and a ground diameter of more than 3 mm. The seedlings are strong and neat, with upright taproots and abundant efficient rhizobia.

[0031] In some embodiments of the present invention, a method for raising seedlings of Orchidaceae is disclosed, comprising the following steps:

[0032] (1) Evenly sowing the germinated Nanyang Ying seeds in the culture matrix, and then covering the Nanyang Ying seeds with the culture matrix, injecting the bacterial solution of the rhizobium strain GZMP-08 into the culture matrix, and then spraying clean water to moisten the culture matrix, placing it under the conditions of temperature of 28° C. to 30° C., humidity of 70% to 80%, and natural light, and inoculating and culturing for 7 days to 10 days; the deposit number of the rhizobium strain GZMP-08 is CCTCC No: M20232371;

[0033] (2) Transplanting the inoculated seedlings into a container bag containing a seedling medium; the seedling medium is loess, peat and vermiculite in a volume ratio of 7±0.1:2±0.1:1±0.1, or loess, peat and vermiculite in a volume ratio of 5±0.1:2±0.1:3±0.1;

[0034] (3) Light and water management during the seedling stage;

[0035] (4) Apply fertilizer 3 months after transplanting the seedlings; prune the roots 4 months after transplanting; and the Nanyangying seedlings can be planted out 5 months after transplanting.

[0036] In some embodiments, the OD of the bacterial solution of the rhizobium strain GZMP-08 in step (1) is 600 The value is 0.8~1.2; every 1cm 2 In the culture matrix, the injection volume of the bacterial solution of the rhizobium strain GZMP-08 is 0.08 mL to 0.12 mL.

[0037] In some embodiments, the OD of the bacterial solution of the rhizobium strain GZMP-08 is 600 The value is 0.9~1.1; every 1cm 2 In the culture medium, the injection volume of the rhizobium strain GZMP-08 bacterial solution is 0.09 mL to 0.11 mL; more preferably, the OD of the rhizobium strain GZMP-08 bacterial solution is 600 The value is 0.95~1.05; every 1cm 2 In the culture matrix, the injection volume of the bacterial solution of the rhizobium strain GZMP-08 is 0.095 mL to 0.105 mL.

[0038] In some embodiments, the inoculation and culturing time in step (1) is 7 to 8 days.

[0039] In some of the embodiments, the volume ratio of loess soil, peat soil and vermiculite in step (2) is 7±0.05:2±0.05:1±0.05, or the volume ratio of loess soil, peat soil and vermiculite is 5±0.05:2±0.05:3±0.05.

[0040] In some embodiments, the container bags in step (2) are placed in a seedling raising frame, and the rows of the container bags are separated by PVC tubes, plastic tubes or foam rods with a volume similar to that of the container bags.

[0041] In some embodiments, in step (4), the seedling raising frame is moved left and right in situ to perform the root cutting treatment.

[0042] In some embodiments, the container bag in step (2) is a plastic container bag, the diameter of the plastic container bag is 5.5 cm to 6.5 cm, and the height of the plastic container bag is 8.5 cm to 9.5 cm.

[0043] In some of the embodiments, in step (2), one seedling is transplanted into each of the container bags, and during transplanting, the root system of the seedling is stretched and the seedling is upright.

[0044] In some of the embodiments, in step (2), before transplanting the seedlings, 0.08% to 0.12% potassium permanganate solution is used to spray the seedling medium until it is completely moistened, and then the medium is placed in the sun for 2 to 3 days.

[0045] In some of the embodiments, the fertilization formula in step (4) is a 0.08% to 0.12% compound fertilizer solution (the total content of N, P2O5, and K2O is greater than 25%).

[0046] In some of the embodiments, step (4) also includes a step of fertilizing 6 to 7 days before transplanting, wherein the fertilizer formula is a 0.08% to 0.12% compound fertilizer solution (total content of N, P2O5, and K2O > 25%), so as to provide sufficient nutrients for the seedlings to adapt to the afforestation environment in the early stage of afforestation.

[0047] In some embodiments, the culture matrix in step (1) is quartz sand, vermiculite, and peat soil in a volume ratio of 0.8-1.2:0.8-1.2:0.8-1.2.

[0048] The present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0049] Embodiment 1 A method for raising seedlings of Nanyang Ying

[0050] In this embodiment, the mixed-line seeds of the Nanyang Ying seed garden produced by the Nanyang Ying improved seed base in Guangdong Province are raised, and the following steps are included:

[0051] 1. Seed germination

[0052] Soak the mixed seeds of Nanyang Ying in 5% potassium permanganate solution for 15 minutes, rinse with clean water, and then pour in 85℃ hot water to soak the seeds. After the water cools naturally to room temperature, continue soaking the seeds for 24 hours, remove the waste seeds floating on the surface, and rinse with clean water twice. Place the moistened filter paper in a clean culture container, evenly place the seeds in it, cover it, and culture it in a 28℃ incubator in the dark. After 2-4 days, the seeds will germinate white radicles (whitening).

[0053] 2. Inoculate seedlings with rhizobia

[0054] Mix quartz sand, vermiculite and peat soil (culture matrix) in a volume ratio of 1:1:1 evenly, sterilize at 121℃ for 1.5h and put them into a container with a filling thickness of 3cm. Sow the germinated seeds evenly in the culture matrix, and then sprinkle a 0.2cm thick layer of the same matrix to cover the seeds. Inject the cultured high-efficiency rhizobium strain GZMP-08 bacterial solution into the matrix, and the bacterial solution injection volume is 1cm 2 About 0.1mL, then spray clean water to moisten the substrate, place it at a temperature of 28℃~30℃, humidity of 75%, and natural light, and inoculate and culture for 7 days. Figure 1 As shown (respectively as Figure 1 A, B and C in the figure).

[0055] The preparation of the bacterial solution of the rhizobium strain GZMP-08 is as follows: the purified high-efficiency rhizobium strain GZMP-08 (classified as Rhizobium sp., with a deposit number of CCTCC No: M 20232371, and the applicant has submitted a deposit certificate of the strain in the prior application: 2024102163064 submitted on February 27, 2024) stored at -20°C is transferred to a YMA liquid culture medium, and placed on a shaker at 28°C and 120 rpm for culture until the bacterial solution is uniformly enriched and appears off-white or milky white. The OD 600 The value of 1.0 can be used for inoculation. The YMA formula is: mannitol 10.0 g / L, sodium glutamate 0.5 g / L, K2HPO4 0.5 g / L, MgSO4·7H2O 0.2 g / L, NaCl 0.2 g / L, yeast powder 0.5 g / L, agar powder 17.5 g / L.

[0056] 3. Transplanting and bagging

[0057] Mix loess soil, peat soil and vermiculite (seedling medium) in a volume ratio of 7:2:1 evenly, use a plastic container bag with a specification of 6cm (diameter) × 9cm (height) to hold the seedling medium, and place it in the seedling frame in alternate rows with foam rods of similar volume, spray the seedling medium with 1% potassium permanganate solution to make the medium in the container completely moist, and then place it in the sun for 2 days.

[0058] Use a bamboo stick to make a hole in the center of the container with a depth of 5 cm and a diameter of 1 cm. Carefully dig out the inoculated seedlings and transplant them, one bag per plant. Try to allow the roots to stretch and the seedlings to stand upright. Fill with seedling medium and compact it lightly, then water thoroughly.

[0059] 4. Light and water management during the seedling stage

[0060] After transplanting, use a PVC support to make a semi-arch with a height of 70cm, cover with film, and maintain humidity at 85% to 95%; cover with a sunshade net (transmittance 80% to 90%) when the sun is strong. After the seedlings have grown for 20 days, open both ends of the film for convection and ventilation, and then gradually remove the film. Within 30 days after removing the film, cover with a sunshade net on sunny days, and water every morning and evening to keep the seedling medium moist. The growth of one-month-old seedlings is as follows: Figure 2 As shown in A.

[0061] 5. Fertilization and root cutting management during the seedling stage

[0062] Three months after transplanting, the seedlings begin to grow rapidly and need to be fertilized for the first time. The fertilizer formula is 0.1% compound fertilizer solution (total content of N, P2O5, K2O> 25%, purchased from the market) for watering. Figure 2 As shown in B.

[0063] Four months after the seedlings are transplanted, they enter a peak growth period, especially when their height grows vigorously and tender shoots sprout rapidly. The seedling frames are moved left and right in situ to perform root cutting to control their height growth and promote lignification of the seedlings.

[0064] At 5 months old, the average seedling height is 31.53cm and the ground diameter is 3.56mm. The seedlings are strong and neat, with upright taproots and abundant high-efficiency rhizobia (the growth trend of 5-month-old seedlings that can be planted in the nursery is shown in the figure below). Figure 2 As shown in C), it meets the requirements for afforestation (according to the Guangdong Provincial Local Standard "Technical Regulations for Seedling Cultivation of Nanyang Ying" (DB44 / T1112-2013), the seedling age of Nanyang Ying seedlings is 6 months old, and the seedling height is 20cm~35cm and the ground diameter is ≥0.25mm to be qualified).

[0065] Embodiment 2 A method for raising seedlings of Nanyang Ying

[0066] In this embodiment, seedlings are grown on mixed-line seeds of the Nanyang Ying seed garden produced by the Nanyang Ying seed base in Guangdong Province. Except that the seedling medium formula in step 3 is loess, peat soil, and vermiculite in a volume ratio of 5:2:3, other steps are the same as in Example 1.

[0067] After 5 months of seedling raising by the seedling raising method of the present invention, the average seedling height is 30.68 cm and the ground diameter is 3.45 mm. The seedlings are strong and neat, the main roots are upright, and the high-efficiency rhizobia are abundant, which meets the requirements for afforestation.

[0068] Comparative Example 1: A method for raising seedlings of Psoralea corylifolia

[0069] The seedling raising method of the Nanyang Ying in this comparative example is a traditional seedling raising method, which is implemented according to the Guangdong Provincial Local Standard "Technical Regulations for Seedling Raising of Nanyang Ying" (DB44 / T 1112-2013), and specifically includes the following steps:

[0070] 1. Seed germination

[0071] Soak the mixed seeds of Nanyang Ying in 5% potassium permanganate solution for 15 minutes, rinse with clean water, and then pour in 85℃ hot water to soak the seeds. After the water cools naturally to room temperature, continue soaking the seeds for 24 hours, remove the waste seeds floating on the surface, and rinse with clean water twice. Put clean and moist gauze in a clean culture container (after germination, the radicles are easy to penetrate the gauze and grow, and the roots are easy to break when planting and taking), evenly place the seeds in it, cover it, and culture it in a 28℃ incubator in the dark. After 2d to 4d, the seeds will germinate white radicles (whitening).

[0072] 2. Seeding of sprouts

[0073] Use a 6cm×9cm plastic container bag, mix 98% loess soil and 2% compound fertilizer in a volume ratio as the matrix, and sow the seeds and seedlings in the center of the matrix, one plant per bag.

[0074] 3. Management during the initial stage of transplanting

[0075] Same as step 4 of Example 1.

[0076] 4. Fertilization and root management

[0077] Fertilize once every 15 days, for a total of 3 to 4 times, preferably with 1% decomposed human feces and urine, or 0.1% high-nitrogen compound fertilizer solution. After each fertilization, drench thoroughly with water to prevent burning of the seedling roots. Loosen the roots of the seedlings in time (move bags) and place them in grades. Apply another fertilizer 7 days before planting.

[0078] Comparative Example 2: A method for raising seedlings of Acacia chinensis

[0079] In the seedling raising method of the Nanyang Jacaranda of this comparative example, except that the method of inoculating rhizobia in step 2 "inoculating seedlings with rhizobia" is seed soaking inoculation, the other steps are the same as those in Example 1.

[0080] 2. Inoculate seedlings with rhizobia

[0081] After germination, the seeds are immersed in the cultured rhizobium GZMP-08 bacterial solution for 2 hours to 3 hours, and then the sprouts are sown in the center of the substrate, one plant per bag.

[0082] Comparative Example 3: A method for raising seedlings of Acacia chinensis

[0083] In the seedling raising method of the Nanyangying of this comparative example, the method of inoculating rhizobia is watering inoculation, which specifically includes the following steps:

[0084] 1. Seed germination

[0085] Same as step 1 in Example 1.

[0086] 2. Seeding of sprouts

[0087] The seedling raising container and substrate are the same as those in Example 1. The germinated seedlings are sown in the center of the substrate, one bag per plant.

[0088] 3. Bacterial culture

[0089] Same as step 2 of Example 1.

[0090] 4. Watering and inoculation

[0091] About 7 days after the seedlings are transplanted into the bags, when the seedlings are upright, the compound pinnate leaves unfold, and the leaf color turns green, with 2 to 4 leaves in number, it indicates that the underground root system has begun to develop. The healthy and surviving seedlings that have been transplanted can be inoculated by watering. Use a pipette to absorb the cultured rhizobium solution and evenly pour 1 ml of it into the matrix around the seedlings for each plant.

[0092] 5. Seedling management

[0093] Same as steps 4 and 5 of Example 1.

[0094] Comparative Example 4: A method for inoculating and raising seedlings of Acacia chinensis

[0095] In this embodiment, seedlings are grown on mixed seeds of the Nanyang Ying seed garden produced by the Nanyang Ying breeding base in Guangdong Province. Except that the seedling medium formula in step 3 is loess, peat soil, and vermiculite in a volume ratio of 4:5:1, other steps are the same as in embodiment 1.

[0096] Comparative Example 5: A method for inoculating and raising seedlings of Acacia chinensis

[0097] In this embodiment, seedlings are grown on mixed seeds of the Nanyang Ying seed garden produced by the Nanyang Ying breeding base in Guangdong Province. Except that the seedling medium formula in step 3 is loess, peat soil, and vermiculite in a volume ratio of 3:3:4, other steps are the same as in embodiment 1.

[0098] Comparative Example 6: A method for inoculating and raising seedlings of Azalea scabra

[0099] In this embodiment, seedlings are grown on mixed seeds of the Nanyang Ying seed garden produced by the Nanyang Ying breeding base in Guangdong Province. Except that the seedling medium formula in step 3 is loess, peat soil, and vermiculite in a volume ratio of 5:4:1, other steps are the same as in Example 1.

[0100] Comparative Example 7: A method for inoculating and raising seedlings of Acacia chinensis

[0101] In this embodiment, seedlings are grown on mixed seeds of the Nanyang Ying seed garden produced by the Nanyang Ying breeding base in Guangdong Province. Except that the seedling medium formula in step 3 is loess, peat soil, and vermiculite in a volume ratio of 3:4:3, other steps are the same as in embodiment 1.

[0102] Comparative Example 8: A method for inoculating and raising seedlings of Azalea scabra

[0103] In this embodiment, seedlings are grown on mixed seeds of the Nanyang Ying seed garden produced by the Nanyang Ying breeding base in Guangdong Province. Except that the seedling medium formula in step 3 is loess, peat soil, and vermiculite in a volume ratio of 4:3:3, other steps are the same as in embodiment 1.

[0104] Comparative Example 9: A method for inoculating and raising seedlings of Nanyang Ying

[0105] In this embodiment, seedlings are grown on mixed-line seeds of Nanyang Ying seed garden produced by Nanyang Ying seed base in Guangdong Province. Except that the seedling medium formula in step 3 is 98% loess + 2% compound fertilizer, other steps are the same as those in embodiment 1.

[0106] Test Example 1 Seedling cultivation results using different rhizobium inoculation methods

[0107] The results of seedling raising methods of Example 1 and Comparative Examples 1 to 3 (5 months) were statistically analyzed, and the seedling height, ground diameter and relative chlorophyll content of each seedling in the nursery were measured. Ten average seedlings with normal growth were selected for each, and the average root length and fresh weight of nodules were measured. The results are shown in Table 1.

[0108] Table 1

[0109]

[0110] From the results in Table 1, it can be seen that compared with the traditional seedling raising method (Comparative Example 1), the seedling height, ground diameter and root length of the seedling raising method of the present invention (Example 1) increased by 56.63%, 39.06% and 29.26% respectively, the fresh weight of the nodules was more than twice that of the traditional seedling raising method, the relative chlorophyll content in the leaves was also greatly increased, the leaf color was dark green, and photosynthesis could be better exerted. The nodulation of the seedlings cultivated by the seedling raising method of the present invention and the seedlings cultivated by the traditional method at 1 month, 3 months and 5 months were compared as follows: Figure 3 As shown in A, B and C, from Figure 3 As can be seen from B, the seedlings cultivated for 3 months by the seedling raising method of the present invention have grown mature nodules inoculated with the efficient rhizobia, which are pink in color, rich in leghemoglobin, and can play a role in efficient nitrogen fixation, while the seedlings cultivated for 3 months by the traditional method have only been inoculated with 3 to 5 very tiny nodules, which do not yet have the nitrogen fixation function.

[0111] The seedling height, ground diameter, average root length and nodule fresh weight of the seedlings in Comparative Example 2 are significantly lower than those in Example 1. The reason may be that the inoculation route of rhizobia is that the rhizobia first secrete NodD protein to recognize the flavonoids secreted by the roots of leguminous plants, inducing the outer cells of the roots to form a pre-infection line, while the inner cortical cells form nodule primordia through mitosis; then, the plant root hairs begin to curl and swell, the tips of the root hairs bend inward to form an infection line and continue to grow toward the cortical tissue, so that the rhizobia enter the nodule primordium to form nodules, thereby establishing a symbiotic relationship. The method of inoculating rhizobia in Comparative Example 2 is the seed soaking inoculation method, that is, the seeds after germination are immersed in the rhizobium solution for 2h to 3h. The disadvantage of this method is that the seeds have just completed germination, the radicle has just become white, and the root system has not yet developed, so the success rate of inoculation is relatively low.

[0112] The method of inoculating rhizobia in Comparative Example 3 is the irrigation inoculation method, that is, after the seedlings are transplanted and bagged, the healthy and surviving seedlings are inoculated by irrigation. The disadvantage of this method is that it requires a large amount of rhizobium solution, and the inoculated solution is easily contaminated and inactivated in an outdoor nursery environment, and the raw materials and labor consumption are high. At the same time, the roots of the seedlings have already grown in the seedling culture medium, and the native inefficient rhizobia in the medium will inevitably compete with the applied target rhizobia for nodulation, thereby affecting the nodulation effect of the target rhizobia. Therefore, the fresh weight of nodules of the seedlings in Comparative Example 3 is also significantly lower than the fresh weight of nodules of the seedlings in Example 1.

[0113] Experimental Example 2: Seedling growth and rhizobium nodulation results using different seedling substrates

[0114] After 5 months of transplanting the seedlings, 3 normal average seedlings were selected in different treatments (Examples 1, 2 and Comparative Examples 4-9) as the plants to be tested. Carefully dig the seedlings out of the matrix, wash them, and then use absorbent paper to control the drying and test them. Use a ruler to measure the height of each seedling and the length of the root system at which the density reaches more than 80% after the root system is naturally stretched (accurate to 0.1 cm), and use an electronic digital display vernier caliper to measure the ground diameter (accurate to 0.01 mm). Pick each nodule and count the number of nodules per plant, and weigh the aboveground part, underground part and total nodule fresh weight of each plant with a 1 / 1000 electronic balance. Then place the aboveground part and underground part of the seedlings in a 105°C oven for 30 minutes, dry them at 85°C to constant weight, and after cooling in a dryer, use an electronic balance to weigh the dry weight of the aboveground part and underground part.

[0115] The results are as follows Figures 4 to 11As shown. There are extremely significant differences (p<0.01) in the growth indicators such as seedling height, ground diameter, average root length, nodule amount and the biomass accumulation indicators such as aboveground fresh weight, root fresh weight, aboveground dry weight and root dry weight of different treatments. Among them, the seedling height, ground diameter and nodule weight of the seedlings grown using the seedling medium of Example 1 and Example 2 are better or significantly better than those of Comparative Examples 4 to 9, and the four biomass accumulation indicators also perform well. Therefore, the seedling medium is loess, peat soil and vermiculite with a volume ratio of 7:2:1 or 5:2:3, which can significantly promote the growth of Nanyangying seedlings and rhizobium nodulation.

[0116] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0117] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for raising seedlings of Acacia chinensis, characterized in that: The following steps are involved: (1) Evenly sowing the germinated seeds of Nanyang jacaranda in a culture medium, injecting a bacterial solution of the rhizobium strain GZMP-08 into the culture medium, keeping the culture medium moist, placing the culture medium at a temperature of 28° C. to 30° C., a humidity of 70% to 80%, and natural light, and inoculating and culturing for 7 days to 10 days; the deposit number of the rhizobium strain GZMP-08 is CCTCC No: M 20232371; The OD of the bacterial solution of the rhizobium strain GZMP-08 600 The value is 0.8~1.2; every 1cm 2 In the culture matrix, the injection amount of the rhizobium strain GZMP-08 bacterial solution is 0.08mL-0.12mL; the culture matrix is ​​quartz sand, vermiculite, and peat soil in a volume ratio of 0.8-1.2:0.8-1.2:0.8-1.2; (2) Transplanting the inoculated seedlings into a container bag containing a seedling medium; the seedling medium is loess, peat and vermiculite in a volume ratio of 7±0.1:2±0.1:1±0.1, or loess, peat and vermiculite in a volume ratio of 5±0.1:2±0.1:3±0.1; (3) Light and water management during the seedling stage; (4) Apply fertilizer 3 months after transplanting the seedlings; prune the roots 4 months after transplanting; and the Nanyangying seedlings can be planted out 5 months after transplanting.

2. The method for raising seedlings of Orchidaceae according to claim 1, characterized in that: The OD of the bacterial solution of the rhizobium strain GZMP-08 in step (1) 600 The value is 0.9~1.1; every 1cm 2 In the culture matrix, the injection volume of the bacterial solution of the rhizobium strain GZMP-08 is 0.09 mL to 0.11 mL.

3. The method for raising seedlings of Orchidaceae according to claim 1, characterized in that: In step (2), the volume ratio of loess soil, peat soil and vermiculite is 7±0.05:2±0.05:1±0.05, or the volume ratio of loess soil, peat soil and vermiculite is 5±0.05:2±0.05:3±0.

05.

4. The method for raising seedlings of Orchidaceae according to any one of claims 1 to 3, characterized in that: The container bags in step (2) are placed in a seedling raising frame, and two adjacent rows of container bags are separated by PVC tubes, plastic tubes or foam rods with a volume similar to that of the container bags.

5. The method for raising seedlings of Orchidaceae according to claim 4, characterized in that: In step (4), the seedling raising frame is moved left and right in situ to perform the root cutting process.

6. The method for raising seedlings of Orchidaceae according to any one of claims 1 to 3, characterized in that: The container bag in step (2) is a plastic container bag, the diameter of the plastic container bag is 5.5 cm to 6.5 cm, and the height of the plastic container bag is 8.5 cm to 9.5 cm.

7. The method for raising seedlings of Orchidaceae according to any one of claims 1 to 3, characterized in that: In step (2), one seedling is transplanted into each container bag, and during transplanting, the root system of the seedling is stretched and the seedling is upright.

8. The method for raising seedlings of Orchidaceae according to any one of claims 1 to 3, characterized in that: In step (2), before transplanting the seedlings, 0.08% to 0.12% potassium permanganate solution is used to spray the seedling medium until it is completely moistened, and then the medium is placed in the sun for 2 to 3 days.

9. The method for raising seedlings of Orchidaceae according to any one of claims 1 to 3, characterized in that: The fertilization formula in step (4) is a 0.08% to 0.12% compound fertilizer solution.

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