Device and method for obtaining fetal trophoblast cells from cervical mucus samples of pregnant women

Abstract

The present invention discloses a device and method for obtaining fetal trophoblast cells from a cervical mucus sample of a pregnant woman in the technical field of in vitro diagnosis, comprising a protective cover, a lower fixing plate fixedly installed at the bottom of the protective cover, a circular ring groove is arranged in the middle position of the lower fixing plate and an upper gear ring is rotatably installed therein, a clamping frame is clamped in the upper gear ring, a plurality of groups of connecting rings are rotatably installed on the lower surface of the clamping frame, positioning tubes are clamped in the connecting rings, limiting rods are arranged at both ends of the lower fixing plate, a plurality of movable frames are slidably connected to the limiting rods, a positioning plate is fixedly installed at the upper end of the movable frame, and a plurality of groups of feeding tubes are arranged on the positioning plate. The present invention provides a device and method for obtaining fetal trophoblast cells from a cervical mucus sample of a pregnant woman, avoiding the trouble of manual mixing, improving the input efficiency, and ensuring the accuracy of the input amount of the immunomagnetic beads and the cell suspension, so that the cell suspension and the immunomagnetic beads are more fully mixed and contacted, thereby improving the capture success rate and the extraction amount.

Description

Technical Field

[0001] The present invention relates to the technical field of in vitro diagnosis, and specifically to a device and method for obtaining fetal trophoblastic cells from cervical mucus samples of pregnant women. Background Art

[0002] In the past three decades, people have gradually begun to be able to separate and purify these trace cells from the embryo from the large number of maternal background cells in cervical mucus samples, and then analyze and detect the biological information they carry. In 2002, Bussani et al. identified trophoblast cells from cervical mucus samples by cell morphology, and isolated trophoblast cells by single-cell micromanipulation. In 2005, Katz-Jaffe et al. used fluorescent immunohistochemistry and micromanipulation techniques to isolate fetal cells from cervical mucus samples of pregnant women. At the same time, Bulmer and other researchers used relatively specific antibodies for trophoblast cells, identified trophoblast cells by immunocytochemical staining technology, and used laser fiber cutting capture devices to capture trophoblast cells. They successfully used PCR methods to perform genotyping of fetal DNA. This method has certain limitations. Generally, a PAP cervical sampling contains at least 1 million cells. Due to the large number of background cells from the mother, the absolute and relative number of trophoblast cells are very small. Usually, there is one trophoblast cell for every 2,000 cells, and there are usually only 500-1,500 trophoblast cells in a single sampling. The number of trophoblast cells that can be collected in the end depends on the total number of cells to be tested. Traditional cell micromanipulation requires making a cell smear for staining first. Limited by the area of ​​the slide, generally only one twentieth of the cervical sampling volume can be taken at a time, containing about 25 trophoblast cells, which is a very small number. A slight fluctuation in the quality of clinical samples will result in extremely low or even no trophoblast cells in the cell smear. Moreover, this separation and capture method mainly relies on manual film reading. It is very difficult for pathologists to find a few positively stained cells among tens of thousands of cells under an optical microscope. It is easy to get tired and has a high rate of missed diagnosis. Manual operation to capture target cells is difficult, the process is cumbersome and time-consuming, and it is difficult to standardize and normalize, which limits its clinical application.

[0003] In the past decade, the development of immunomagnetic bead technology has brought hope to TRIC. Immunomagnetic bead technology is to modify the surface of nanoscale magnetic spheres with trophoblast-specific antibodies, which can specifically recognize and bind to antigens on the surface of trophoblast cells, thereby achieving the purpose of identifying and capturing cells. This method has certain standardized and programmed characteristics and has achieved certain success, but the manual operation process is still relatively complicated. In the past two years, some people have also done some repeated verification experiments and believed that TRIC is too few and unstable, with poor repeatability, and it is impossible to stably and reliably obtain trophoblast cells through standard immunomagnetic bead separation and capture technology. Therefore, it cannot replace the current invasive prenatal screening and diagnosis methods. Summary of the invention

[0004] The technical problem of the present invention is to provide a device and method for obtaining fetal trophoblastic cells from cervical mucus samples of pregnant women, so as to provide a device for automatically mixing cell suspension and immunomagnetic beads, avoiding the trouble of manual mixing and improving input efficiency. In addition, it ensures the accuracy of the input amount of immunomagnetic beads and cell suspension, so that the cell suspension and immunomagnetic beads are mixed and contacted more fully, thereby improving the capture success rate and extraction amount.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a device for obtaining fetal trophoblast cells from a cervical mucus sample of a pregnant woman, comprising a protective cover, a lower fixing plate fixedly installed at the bottom of the protective cover, a circular ring groove is provided in the middle of the lower fixing plate and an upper gear ring is rotatably installed therein, a clamping frame is clamped in the upper gear ring, a plurality of connecting rings are rotatably installed on the lower surface of the clamping frame, and positioning tubes are clamped in the connecting rings, limiting rods are provided at both ends of the lower fixing plate, a plurality of movable frames are slidably connected to the limiting rods, a positioning plate is fixedly installed at the upper end of the movable frame, a plurality of feeding tubes are provided on the positioning plate, a plurality of injection tubes are provided between the movable frames, a transmission assembly is provided between the injection tube and the feeding tube, and the transmission assembly can drive the injection tube to move between the feeding tube and the positioning tube and automatically adjust the angle during the movement;

[0006] The transmission assembly includes a side fixing frame, the side fixing frame is fixedly mounted on one side of the movable frame, a threaded rod is fixedly mounted on the side fixing frame, a movable part is arranged on the threaded rod, a connecting shaft is rotatably mounted on the movable part, an end of the connecting shaft away from the movable frame is clamped with a moving seat, the injection pipe is fixedly mounted on the moving seat, the injection pipe corresponds to the feeding pipe position one by one, an introduction part is fixedly mounted on the upper end of the injection pipe, a connecting plate is fixedly mounted at the middle position inside the movable frame, teeth are fixedly mounted on the connecting plate, a side gear is fixedly mounted on one end of the connecting shaft close to the movable frame, and the side gear can mesh with the teeth;

[0007] The positions of the movable parts corresponding to the threaded rods are all rotatably mounted with threaded parts, the upper ends of the threaded parts are all fixedly mounted with transmission wheels, the outer shells of the transmission wheels are connected with transmission belts, the upper surface of the transmission wheels on one side is fixedly mounted with a limit gear, the upper surface of the movable parts is rotatably mounted with a driving gear, and the driving gear is meshed with the limit gear;

[0008] The upper gear ring is rotatably mounted on the upper surface of the lower fixed plate, a limit plate is symmetrically mounted on the inner side of the upper gear ring, and a limit piece is fixedly mounted on the lower surface of the limit plate. Limit grooves are provided at both ends of the clamping frame corresponding to the position of the limit piece, a side mounting plate is fixedly mounted on one side of the protective cover, and a driving gear is rotatably mounted on the side mounting plate, and the driving gear is meshed with the upper gear ring;

[0009] The lower surface of the connecting ring is fixedly installed with a clamping tooth, the outer surface of the clamping tooth is connected with a transmission chain, the lower surface of the lower fixed plate is fixedly installed with a fixed tooth ring, and the lower surface of the edge of the clamping tooth is fixedly installed with a moving gear, and the moving gear is meshed with the fixed tooth ring;

[0010] A material guide is fixedly installed at the upper end of the feeding pipe, a lower connecting piece is fixedly installed at the lower end of the feeding pipe, a plurality of elastic pieces are fixedly installed in the lower connecting piece, a sealing plate is fixedly installed at the upper end of the elastic piece, the sealing plate can seal the lower end of the lower connecting piece, an introduction piece is fixedly installed at the upper end of the injection pipe, the upper end of the introduction piece can be introduced into the lower connecting piece, and a plurality of overflow grooves are opened on the outer side of the introduction piece;

[0011] An upper fixed cover is fixedly mounted on the upper surface of the protective cover, and a slide groove is provided on the upper fixed cover at a position corresponding to the feeding pipe. A driving motor is fixedly mounted on the side mounting plate, and an output end of the driving motor is fixedly connected to the driving gear.

[0012] A refrigeration device is arranged inside the lower fixed plate, a spring valve is arranged at the lower end of the injection pipe, and an elastic rod of the spring valve is led out from the lower end of the injection pipe in a natural state.

[0013] As a further solution of the present invention, a rotating motor is fixedly mounted on the movable member, an output end of the rotating motor is fixedly connected to the driving gear, and the movable frame and the limiting rod are dampingly connected.

[0014] A method for obtaining fetal trophoblastic cells from a cervical mucus sample of a pregnant woman, wherein the specific steps of the method for obtaining fetal trophoblastic cells from a cervical mucus sample of a pregnant woman are as follows:

[0015] Step 1: injecting the prepared cell suspension into the feeding tube;

[0016] Step 2: Move a group of the movable frames to drive the injection pipe to move below the feeding pipe;

[0017] Step 3: driving the injection tube to move upward so that the introduction piece enters into the lower connecting piece, so that the cell suspension enters into the injection tube;

[0018] Step 4: moving the movable frame to move the injection tube with the cell suspension to above the positioning tube;

[0019] Step 5: driving the injection pipe to move downward, and the injection pipe automatically tilts and is introduced into the positioning pipe after moving downward, and the lower end of the injection pipe is in contact with the inner wall of the positioning pipe;

[0020] Step 6: The positioning tube squeezes the spring valve at the bottom of the injection tube so that the cell suspension is injected into the positioning tube;

[0021] Step 7: Then adding immunomagnetic beads into the positioning tube;

[0022] Step 8: Then drive the upper gear ring to rotate so that the immunomagnetic beads and the cell suspension are fully contacted and mixed;

[0023] Step nine: then remove the card-joint frame from between the upper gear rings, and place the card-joint frame into the cell sorter for sorting.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1. In the present invention, cell suspension and immune magnetic beads are sequentially injected through an injection tube, and the spring valve is squeezed upward to open the lower end of the injection tube, so that the cell suspension can flow out from the lower end of the injection tube, and then flow into the bottom of the positioning tube along the inner wall of the positioning tube, ensuring that the injected cell suspension can be slowly injected into the interior of the positioning tube to maintain the stability of the cell suspension. After the cell suspension is injected, the immune magnetic beads are injected into the positioning tube again through the above steps, so that both the immune magnetic beads and the cell suspension do not need to be manually injected into the positioning tube. By controlling the opening angle of the spring valve, the amount of cell suspension and immune cells injected into the positioning tube can be accurately controlled, ensuring that the amount of each component injected into the positioning tube is more accurate, avoiding the problem of large errors in manual injection, and multiple positioning tube experimental groups can be operated at the same time to improve the extraction efficiency.

[0026] 2. In the present invention, the rotation of the upper gear ring drives the rotation of the clamping frame, the rotation of the clamping frame drives the rotation of the positioning tube, the rotation of the clamping frame drives the rotation of the mobile gear, the mobile gear interacts with the fixed gear ring to drive the mobile gear to rotate, the rotation of the mobile gear drives the edge clamping teeth to rotate, the clamping teeth interact with the transmission chain to drive multiple groups of connecting rings to rotate at the same time, the rotation of the connecting rings drives the multiple groups of positioning tubes to rotate in the clamping frame, so that the positioning tubes rotate while the upper gear ring rotates, so that the cell suspension in the positioning tubes and the immunomagnetic beads are fully in contact, thereby improving the capture success rate and extraction amount. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for describing the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0028] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0029] Figure 2 This is a schematic diagram of the structure of the present invention when viewed from the bottom up;

[0030] Figure 3 For the present invention Figure 2 A partial schematic diagram of the structure at A in the middle;

[0031] Figure 4 The internal structure of the present invention is shown in FIG. Figure 1 ;

[0032] Figure 5 For the present invention Figure 4 A partial schematic diagram of the structure at B in the middle;

[0033] Figure 6 For the present invention Figure 4 A partial schematic diagram of the structure at C in the middle;

[0034] Figure 7 The internal structure of the present invention is shown in FIG. Figure 2 ;

[0035] Figure 8 For the present invention Figure 7 A partial schematic diagram of the structure at D in the middle;

[0036] Fig. 9 It is a structural cross-sectional view of the present invention;

[0037] Fig.10 For the present invention Fig. 9 Partial schematic diagram of the structure at E in the middle.

[0038] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0039] 1. Protective cover; 2. Upper fixed cover; 3. Feeding pipe; 4. Material guide; 5. Side mounting plate; 6. Active gear; 7. Lower fixed plate; 8. Fixed gear ring; 9. Snap-on frame; 10. Positioning pipe; 11. Transmission chain; 12. Snap-on teeth; 13. Limiting piece; 14. Connecting ring; 15. Injection pipe; 16. Moving gear; 17. Upper gear ring; 18. Limiting rod; 19. Movable frame; 20. Positioning plate; 21. Side fixed frame; 22. Threaded rod; 23. Connecting plate; 24. Teeth; 25. Limiting plate; 26. Movable piece; 27. Moving seat; 28. Transmission belt; 29. ​​Driving gear; 30. Transmission wheel; 31. Limiting gear; 32. Threaded piece; 33. Connecting shaft; 34. Side gear; 35. Introducing piece; 36. Lower connecting piece; 37. Sealing plate; 38. Elastic piece. DETAILED DESCRIPTION

[0040] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] See also Figure 1-Figure 10 The present invention provides a technical solution: a device for obtaining fetal trophoblast cells from a cervical mucus sample of a pregnant woman, comprising a protective cover 1, a lower fixing plate 7 is fixedly installed at the bottom of the protective cover 1, a circular ring groove is arranged in the middle position of the lower fixing plate 7 and an upper gear ring 17 is rotatably installed therein, a clamping frame 9 is clamped in the upper gear ring 17, a plurality of connecting rings 14 are rotatably installed on the lower surface of the clamping frame 9, a positioning tube 10 is clamped in each of the connecting rings 14, limiting rods 18 are arranged at both ends of the lower fixing plate 7, a plurality of movable frames 19 are slidably connected to the limiting rods 18, a positioning plate 20 is fixedly installed at the upper end of the movable frame 19, a plurality of feeding tubes 3 are arranged on the positioning plate 20, a plurality of injection tubes 15 are arranged between the movable frames 19, a transmission assembly is arranged between the injection tube 15 and the feeding tube 3, and the transmission assembly can drive the injection tube 15 to move between the feeding tube 3 and the positioning tube 10 and automatically adjust the angle during the movement.

[0042] During operation, the prepared cell suspension is injected into the feeding tube 3 in the present invention, and then a group of movable frames 19 are moved to drive the injection tube 15 thereon to move to the bottom of the feeding tube 3, and the injection tube 15 is driven to move upward so that the introduction member 35 enters the lower connecting member 36, so that the cell suspension enters the injection tube 15 (the rotation motor drives the driving gear 29 to rotate, the driving gear 29 rotates to drive the limit gear 31 to rotate, the limit gear 31 rotates to drive the transmission wheel 30 to rotate, the transmission wheel 30 drives the threaded member 32 to rotate through the transmission belt 28, the threaded member 32 rotates and interacts with the threaded rod 22, thereby driving the movable member 26 to move upward, and the movable member 26 moves upward The injection tube 15 is driven to move upward, and the upper end guide piece 35 of the injection tube 15 enters into the lower connecting piece 36, so that the guide piece 35 squeezes the sealing plate 37, so that the elastic piece 38 stretches the sealing plate 37 and moves upward, thereby allowing the raw material in the feeding tube 3 to enter the injection tube 15 along the overflow groove of the guide piece 35). Then, the movable frame 19 is moved to move the injection tube 15 with the cell suspension to the top of the positioning tube 10, and the injection tube 15 is driven to move downward. After the injection tube 15 moves downward, it automatically tilts and is introduced into the positioning tube 10, and the lower end of the injection tube 15 is against the inner wall of the positioning tube 10 (the rotating motor rotates in the opposite direction to drive the movable piece 26 to move downward, and the movable piece 26 moves downward. The movement drives the connecting shaft 33 and the moving seat 27 to move downward, thereby driving the injection tube 15 to move downward. During the downward movement of the connecting shaft 33, the side gear 34 interacts with the teeth 24. The side gear 34 rotates under the action of the teeth 24, thereby causing the moving seat 27 to rotate. The rotation of the moving seat 27 causes the lower end of the injection tube 15 to tilt. After tilting, the injection tube 15 continues to move downward, so that the lower end of the injection tube 15 is introduced into the interior of the positioning tube 10). The positioning tube 10 squeezes the spring valve at the bottom of the injection tube 15 so that the cell suspension is injected into the positioning tube 10. The spring valve squeezes upward to open the lower end of the injection tube 15, so that the cell suspension can flow out from the lower end of the injection tube 15. Then it flows along the inner wall of the positioning tube 10 to the bottom of the positioning tube 10, ensuring that the injected cell suspension can be slowly injected into the interior of the positioning tube 10 to maintain the stability of the cell suspension. After the cell suspension is injected, the immune magnetic beads are injected into the positioning tube 10 again through the above steps, so that neither the immune magnetic beads nor the cell suspension need to be manually injected into the positioning tube 10. By controlling the opening angle of the spring valve, the amount of cell suspension and immune cells injected into the positioning tube 10 can be accurately controlled to ensure that the dosage of each component into the positioning tube 10 is more accurate, avoiding the problem of large errors in manual injection, and multiple positioning tube 10 experimental groups can be operated at the same time to improve the extraction efficiency.

[0043] In the present invention, cell suspension and immune magnetic beads are sequentially injected through the injection tube 15, and the spring valve causes the squeezing to move upward so that the lower end of the injection tube 15 is opened, so that the cell suspension can flow out from the lower end of the injection tube 15, and then flow into the bottom of the positioning tube 10 along the inner wall of the positioning tube 10, ensuring that the injected cell suspension can be slowly injected into the interior of the positioning tube 10 to maintain the stability of the cell suspension. After the cell suspension is injected, the immune magnetic beads are injected into the positioning tube 10 again through the above steps, so that both the immune magnetic beads and the cell suspension do not need to be manually injected into the positioning tube 10, and the amount of the cell suspension and immune cells injected into the positioning tube 10 can be accurately controlled by controlling the opening angle of the spring valve, ensuring that the dosage of each component injected into the positioning tube 10 is more accurate, avoiding the problem of large errors in manual injection, and multiple groups of positioning tubes 10 experimental groups can be operated simultaneously to improve the extraction efficiency.

[0044] As a further solution of the present invention, the transmission assembly includes a side fixed frame 21, which is fixedly installed on one side of the movable frame 19, a threaded rod 22 is fixedly installed on the side fixed frame 21, a movable part 26 is arranged on the threaded rod 22, a connecting shaft 33 is rotatably installed on the movable part 26, and a movable seat 27 is clamped at the end of the connecting shaft 33 away from the movable frame 19, the injection pipe 15 is fixedly installed on the movable seat 27, the injection pipe 15 corresponds to the feeding pipe 3 in position one by one, an introduction part 35 is fixedly installed on the upper end of the injection pipe 15, a connecting plate 23 is fixedly installed at the middle position on the inner side of the movable frame 19, teeth 24 are fixedly installed on the connecting plate 23, and a side gear 34 is fixedly installed on the end of the connecting shaft 33 close to the movable frame 19, and the side gear 34 can engage with the teeth 24.

[0045] During operation, the rotating motor rotates in the reverse direction to drive the movable part 26 to move downward, and the downward movement of the movable part 26 drives the connecting shaft 33 and the moving seat 27 to move downward, thereby driving the injection pipe 15 to move downward. During the downward movement of the connecting shaft 33, the side gear 34 interacts with the teeth 24, and the side gear 34 rotates under the action of the teeth 24, thereby causing the moving seat 27 to rotate. The rotation of the moving seat 27 causes the lower end of the injection pipe 15 to tilt. After tilting, the injection pipe 15 continues to move downward, so that the lower end of the injection pipe 15 is introduced into the interior of the positioning pipe 10.

[0046] As a further solution of the present invention, a threaded member 32 is rotatably installed at the position corresponding to the threaded rod 22 on the movable part 26, and a transmission wheel 30 is fixedly installed on the upper end of the threaded member 32. A transmission belt 28 is connected to the outer sleeve of the transmission wheel 30. A limiting gear 31 is fixedly installed on the upper surface of the transmission wheel 30 on one side, and a driving gear 29 is rotatably installed on the upper surface of the movable part 26, and the driving gear 29 is meshed with the limiting gear 31.

[0047] During operation, the rotating motor drives the driving gear 29 to rotate, the driving gear 29 drives the limit gear 31 to rotate, the limit gear 31 drives the transmission wheel 30 to rotate, the transmission wheel 30 drives the threaded member 32 to rotate through the transmission belt 28, the threaded member 32 rotates and interacts with the threaded rod 22, thereby driving the movable member 26 to move upward, the movable member 26 moves upward and drives the injection pipe 15 to move upward, the introduction member 35 at the upper end of the injection pipe 15 enters into the lower connecting member 36, so that the introduction member 35 squeezes the sealing plate 37, so that the elastic member 38 extends the sealing plate 37 to move upward, and then the raw material in the feeding pipe 3 enters into the injection pipe 15 along the overflow groove of the introduction member 35.

[0048] As a further solution of the present invention, the upper gear ring 17 is rotatably mounted on the upper surface of the lower fixed plate 7, and a limiting plate 25 is symmetrically mounted on the inner side of the upper gear ring 17. The limiting member 13 is fixedly mounted on the lower surface of the limiting plate 25. Limiting grooves are provided at both ends of the clamping frame 9 corresponding to the position of the limiting member 13. A side mounting plate 5 is fixedly mounted on one side of the protective cover 1, and a driving gear 6 is rotatably mounted on the side mounting plate 5, and the driving gear 6 is meshed with the upper gear ring 17.

[0049] During operation, the upper gear ring 17 rotates to drive the clamping frame 9 to rotate, the clamping frame 9 rotates to drive the positioning tube 10 to rotate, the clamping frame 9 rotates to drive the moving gear 16 to rotate, the moving gear 16 interacts with the fixed gear ring 8 to drive the moving gear 16 to rotate, the moving gear 16 rotates to drive the edge clamping teeth 12 to rotate, the clamping teeth 12 interact with the transmission chain 11 to drive multiple groups of connecting rings 14 to rotate at the same time, the connection rings 14 rotate to drive multiple groups of positioning tubes 10 to rotate in the clamping frame 9, so that the positioning tube 10 rotates while the upper gear ring 17 rotates, so that the cell suspension in the positioning tube 10 and the immunomagnetic beads are fully in contact, thereby improving the capture success rate and extraction amount.

[0050] As a further solution of the present invention, the lower surface of the connecting ring 14 is fixedly installed with a snap-fitting tooth 12, the outer outer surface of the snap-fitting tooth 12 is connected with a transmission chain 11, the lower surface of the lower fixed plate 7 is fixedly installed with a fixed gear ring 8, and the lower surface of the edge snap-fitting tooth 12 is fixedly installed with a moving gear 16, which is meshed with the fixed gear ring 8.

[0051] During operation, the engaging teeth 12 interact with the transmission chain 11 to drive the multiple sets of connecting rings 14 to rotate simultaneously. The rotation of the connecting rings 14 drives the multiple sets of positioning tubes 10 to rotate in the engaging frame 9, so that the positioning tubes 10 rotate while rotating with the upper gear ring 17.

[0052] As a further solution of the present invention, a material guide member 4 is fixedly installed on the upper end of the feeding pipe 3, a lower connecting member 36 is fixedly installed on the lower end of the feeding pipe 3, a plurality of elastic members 38 are fixedly installed in the lower connecting member 36, a sealing plate 37 is fixedly installed on the upper end of the elastic member 38, and the sealing plate 37 can seal the lower end of the lower connecting member 36, an introduction member 35 is fixedly installed on the upper end of the injection pipe 15, the upper end of the introduction member 35 can be introduced into the lower connecting member 36, and a plurality of overflow grooves are provided on the outer side of the introduction member 35.

[0053] During operation, the movable part 26 moves upward, driving the injection pipe 15 upward, and the introduction part 35 at the upper end of the injection pipe 15 enters into the lower connecting part 36, so that the introduction part 35 squeezes the sealing plate 37, and the elastic part 38 stretches the sealing plate 37 to move upward, thereby allowing the raw materials in the feeding pipe 3 to enter the injection pipe 15 along the overflow groove of the introduction part 35.

[0054] As a further solution of the present invention, an upper fixed cover 2 is fixedly installed on the upper surface of the protective cover 1, and slide grooves are provided on the upper fixed cover 2 at positions corresponding to the feeding pipe 3. A driving motor is fixedly installed on the side mounting plate 5, and the output end of the driving motor is fixedly connected to the driving gear 6.

[0055] As a further solution of the present invention, a refrigeration device is arranged inside the lower fixed plate 7, a spring valve is arranged at the lower end of the injection pipe 15, and the elastic rod of the spring valve is led out from the lower end of the injection pipe 15 in a natural state.

[0056] As a further solution of the present invention, a rotating motor is fixedly mounted on the movable member 26, the output end of the rotating motor is fixedly connected to the driving gear 29, and the movable frame 19 and the limiting rod 18 are connected in a damping manner.

[0057] A method for obtaining fetal trophoblastic cells from a cervical mucus sample of a pregnant woman, wherein the specific steps of the method for obtaining fetal trophoblastic cells from a cervical mucus sample of a pregnant woman are as follows:

[0058] Step 1: injecting the prepared cell suspension into the feeding tube 3;

[0059] Step 2: Move a set of movable frames 19 to drive the injection pipe 15 to move below the feeding pipe 3;

[0060] Step 3: driving the injection tube 15 to move upward so that the introduction member 35 enters the lower connecting member 36, so that the cell suspension enters the injection tube 15;

[0061] Step 4: Move the movable frame 19 to move the injection tube 15 with the cell suspension to above the positioning tube 10;

[0062] Step 5: driving the injection pipe 15 to move downward, the injection pipe 15 automatically tilts and is introduced into the positioning pipe 10 after moving downward, and the lower end of the injection pipe 15 is in contact with the inner wall of the positioning pipe 10;

[0063] Step 6: The positioning tube 10 squeezes the spring valve at the bottom of the injection tube 15 so that the cell suspension is injected into the positioning tube 10;

[0064] Step 7: Then add immunomagnetic beads into the positioning tube 10;

[0065] Step 8: Then drive the upper gear ring 17 to rotate so that the immunomagnetic beads and the cell suspension are fully contacted and mixed;

[0066] Step nine: then remove the clamping frame 9 from between the upper gear rings 17, and place the clamping frame 9 into the cell sorter for sorting.

Claims

1. A device for obtaining fetal trophoblastic cells from a sample of cervical mucus of a pregnant woman, comprising a protective cover (1), characterized in that: A lower fixing plate (7) is fixedly installed at the bottom of the protective cover (1), a circular ring groove is provided in the middle of the lower fixing plate (7) and an upper gear ring (17) is rotatably installed therein, a clamping frame (9) is clamped in the upper gear ring (17), a plurality of connecting rings (14) are rotatably installed on the lower surface of the clamping frame (9), and a positioning tube (10) is clamped in each of the connecting rings (14), and limiting rods (18) are provided at both ends of the lower fixing plate (7), and the upper limit rods (18) are provided with a plurality of connecting rings (14) and a plurality of connecting rings (14 ... A plurality of movable frames (19) are slidably connected, a positioning plate (20) is fixedly mounted on the upper end of the movable frame (19), a plurality of groups of feeding pipes (3) are arranged on the positioning plate (20), a plurality of groups of injection pipes (15) are arranged between the movable frames (19), a transmission assembly is arranged between the injection pipes (15) and the feeding pipes (3), and the transmission assembly can drive the injection pipes (15) to move between the feeding pipes (3) and the positioning pipes (10) and automatically adjust the angle during the movement; The transmission assembly comprises a side fixing frame (21), the side fixing frame (21) being fixedly mounted on one side of the movable frame (19), a threaded rod (22) being fixedly mounted on the side fixing frame (21), a movable member (26) being provided on the threaded rod (22), a connecting shaft (33) being rotatably mounted on each of the movable members (26), a movable seat (27) being clamped at one end of the connecting shaft (33) away from the movable frame (19), and the injection pipe (15) being fixedly mounted on the movable frame. On the seat (27), the injection pipe (15) and the feeding pipe (3) are in one-to-one correspondence, an introduction piece (35) is fixedly installed on the upper end of the injection pipe (15), a connecting plate (23) is fixedly installed at the middle position inside the movable frame (19), teeth (24) are fixedly installed on the connecting plate (23), and a side gear (34) is fixedly installed at one end of the connecting shaft (33) close to the movable frame (19), and the side gear (34) can mesh with the teeth (24); A threaded member (32) is rotatably mounted on the movable member (26) at a position corresponding to the threaded rod (22); a transmission wheel (30) is fixedly mounted on the upper end of the threaded member (32); a transmission belt (28) is connected to the outer surface of the transmission wheel (30); a limit gear (31) is fixedly mounted on the upper surface of one side of the transmission wheel (30); a driving gear (29) is rotatably mounted on the upper surface of the movable member (26); the driving gear (29) is meshed with the limit gear (31); The upper gear ring (17) is rotatably mounted on the upper surface of the lower fixed plate (7); a limiting plate (25) is symmetrically mounted on the inner side of the upper gear ring (17); a limiting member (13) is fixedly mounted on the lower surface of the limiting plate (25); limiting grooves are provided at both ends of the clamping frame (9) corresponding to the position of the limiting member (13); a side mounting plate (5) is fixedly mounted on one side of the protective cover (1); a driving gear (6) is rotatably mounted on the side mounting plate (5); and the driving gear (6) is meshed with the upper gear ring (17); The lower surface of the connecting ring (14) is fixedly mounted with a snap-fitting tooth (12), the outer surface of the snap-fitting tooth (12) is connected with a transmission chain (11), the lower surface of the lower fixed plate (7) is fixedly mounted with a fixed toothed ring (8), and the lower surface of the snap-fitting tooth (12) is fixedly mounted with a movable gear (16), and the movable gear (16) is meshed with the fixed toothed ring (8); A material guide member (4) is fixedly mounted on the upper end of the feeding pipe (3), a lower connecting member (36) is fixedly mounted on the lower end of the feeding pipe (3), a plurality of elastic members (38) are fixedly mounted inside the lower connecting member (36), a sealing plate (37) is fixedly mounted on the upper end of the elastic member (38), the sealing plate (37) can seal the lower end of the lower connecting member (36), an introduction member (35) is fixedly mounted on the upper end of the injection pipe (15), the upper end of the introduction member (35) can be introduced into the lower connecting member (36), and a plurality of overflow grooves are provided on the outer side of the introduction member (35); An upper fixed cover (2) is fixedly mounted on the upper surface of the protective cover (1), a slide groove is provided on the upper fixed cover (2) at a position corresponding to the feeding pipe (3), a driving motor is fixedly mounted on the side mounting plate (5), and an output end of the driving motor is fixedly connected to the driving gear (6); A refrigeration device is arranged inside the lower fixed plate (7), and a spring valve is arranged at the lower end of the injection pipe (15). The elastic rod of the spring valve is led out from the lower end of the injection pipe (15) in a natural state.

2. The device for obtaining fetal trophoblastic cells from a pregnant woman's cervical mucus sample according to claim 1, characterized in that: A rotating motor is fixedly mounted on the movable member (26), the output end of the rotating motor is fixedly connected to the driving gear (29), and the movable frame (19) and the limiting rod (18) are connected in a damping manner.

3. A method for obtaining fetal trophoblastic cells from a cervical mucus sample of a pregnant woman, applicable to the device for obtaining fetal trophoblastic cells from a cervical mucus sample of a pregnant woman according to any one of claims 1 to 2, characterized in that: The specific steps of the method for obtaining fetal trophoblastic cells from a pregnant woman's cervical mucus sample are as follows: Step 1: injecting the prepared cell suspension into the feeding tube (3); Step 2: Move a group of the movable frames (19) to drive the injection pipe (15) to move below the feeding pipe (3); Step 3: driving the injection tube (15) to move upward so that the introduction member (35) enters the lower connecting member (36), so that the cell suspension enters the injection tube (15); Step 4: moving the movable frame (19) to move the injection tube (15) with the cell suspension to above the positioning tube (10); Step 5: driving the injection pipe (15) to move downward, and the injection pipe (15) automatically tilts and is introduced into the positioning pipe (10) after moving downward, and the lower end of the injection pipe (15) is in contact with the inner wall of the positioning pipe (10); Step 6: The positioning tube (10) squeezes the spring valve at the bottom of the injection tube (15) so that the cell suspension is injected into the positioning tube (10); Step 7: Then, add immunomagnetic beads into the positioning tube (10); Step 8: Then drive the upper gear ring (17) to rotate so that the immunomagnetic beads and the cell suspension are fully contacted and mixed; Step nine: then remove the clamping frame (9) from between the upper gear rings (17), and place the clamping frame (9) into a cell sorter for sorting.

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