A composition for improving oily skin and its use in skin care

Abstract

The application belongs to the technical field of cosmetics, and particularly relates to a composition for improving oily skin and application thereof in skin care. The composition for improving oily skin comprises 0.1%-2% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 1%-3% evening primrose oil, 1%-3% isononyl isononanoate, 1%-3% dimethicone, 0.05%-0.2% sodium hyaluronate, 0.5%-1% D-mannitol, 1%-3% fucoidan, 0.01%-0.03% oligoisomaltose, and the rest is water. The application firstly studies the physiological characteristics and skin flora characteristics of the oily skin population, and on this basis, the raw materials are screened based on the proliferation law of the raw materials for the large abundance of bacteria of the oily skin, the safety of the formula is further verified, and the skin improvement after the population uses the formula, so that the application has a wide application prospect.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a composition for improving oily skin and application thereof in skin care. BACKGROUND

[0002] The information disclosed in this Background section is only for the purpose of increasing an understanding of the general background of the application and does not necessarily constitute an admission or a recognition that the information forms part of the prior art already known to a person of ordinary skill in the art.

[0003] Skin oiliness has been a problem that plagues many young people. According to statistics, the prevalence rate of oily skin in the Chinese population is 25.6%. The clinical characteristics of oily skin are excessive sebum secretion, often accompanied by acne, irregular menstruation, and high skin sensitivity. Many factors affect the occurrence of oily skin. In addition to genetic factors, sebum secretion is also related to behaviors such as staying up late, poor dietary structure, and living environment. Although the number of sebaceous glands in the human body remains basically unchanged with age, the sebum secretion rate reaches the highest in the young population aged 15-35 years old, and then gradually decreases. Therefore, the young population is a high-risk group for oily skin and may face a series of problems caused by excessive sebum secretion. This difference in sebum secretion at different stages of life may be related to endogenous hormonal changes. The living environment also has a great impact on the amount of sebum secretion.

[0004] Generally speaking, oily skin has good tolerance to sunlight and environmental bad stimuli, wrinkles appear late and are coarse wrinkles, but oily skin is easily invaded by microorganisms (such as Propionibacterium acnes, Staphylococcus, and Pityrosporum) and thus suffers from skin diseases such as acne, folliculitis, and seborrheic dermatitis that affect appearance. The microbial barrier is the first biological barrier for the skin to resist external adverse invasion. Excessive production of oil can easily lead to excessive proliferation of lipophilic microorganisms, which compete for limited living space and nutritional resources on the skin, resulting in changes in species richness and disorder of species composition, and thus triggering various skin problems. Therefore, maintaining a stable skin microbiota is a prerequisite for skin health.

[0005] Oily skin population has always been widely concerned by the skin care industry, and some species targeting oily skin population have been studied. However, the inventors found that no one has systematically studied the correlation between the composition of the microbiota of oily skin population and skin physiological parameters, and more specifically, no one has targetedly solved the problems of oily population from the perspective of maintaining the balance of the microecological balance of skin care products. SUMMARY

[0006] In view of the deficiencies in the prior art, the present invention aims to provide a composition for improving oily skin and its application in skin care. The present invention adopts a method combining modern sequencing technology means and traditional microbial culture to find the flora differences of oily skin crowds, and uses three strains of bacteria with higher abundance on the skin (Staphylococcus epidermidis, Propionibacterium acnes and Staphylococcus hominis) as indicator bacteria to screen the common raw materials of cosmetics (oils and sugar raw materials), and finally screens out a group of compositions that can effectively improve the troubles of oily skin crowds and are friendly to microecology. Based on the above research results, the present invention is completed.

[0007] In order to achieve the above technical objectives, the technical solutions provided by the present invention are as follows:

[0008] The first aspect of the present invention provides a composition for improving oily skin, wherein the composition for improving oily skin comprises the following components in weight fractions:

[0009] 0.1%-2% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 1%-3% evening primrose oil, 1%-3% isononyl isononanoate, 1%-3% dimethicone, 0.05%-0.2% sodium hyaluronate, 0.5%-1% D-mannitol, 1%-3% fucoidan, 0.01%-0.03% isomaltooligosaccharide, the balance is water;

[0010] Wherein, the molecular weight of the sodium hyaluronate is 1-2 million Da.

[0011] A second aspect of the present invention provides the use of the above-mentioned composition for improving oily skin in the preparation of cosmetics. Experiments have demonstrated that the composition can significantly improve excessive oiliness and sebum secretion, is friendly to skin flora, and reduces potential inflammatory reactions in the skin.

[0012] The third aspect of the present invention provides a cosmetic comprising at least the above composition for improving oily skin.

[0013] The cosmetics may be skin care products.

[0014] A fourth aspect of the present invention provides a method for preparing the above-mentioned cosmetic, comprising mixing the raw material components of the composition for improving oily skin; or mixing the composition for improving oily skin with other raw material components.

[0015] A fifth aspect of the present invention provides use of the above composition and / or cosmetic for improving oily skin in any one or more of the following:

[0016] (a) Effectively improve the composition of skin flora, increase skin species diversity and richness, and balance the skin microecology;

[0017] (b) Improve sebum secretion, increase skin moisture content, control transepidermal water loss rate and reduce a value;

[0018] (c) Reduce hemoglobin content, reduce the possibility of skin inflammation, and thus reduce the risk of skin further deteriorating into oily and acne-prone skin.

[0019] Beneficial technical effects of one or more of the above technical solutions:

[0020] The above technical solution analyzes the changes in bacterial flora characteristics. This patent screens three bacteria with higher abundance from oily skin as indicator bacteria to screen different types of commonly used cosmetic raw materials. The sugar and oil raw materials that can inhibit the growth of Staphylococcus epidermidis and Propionibacterium acnes and promote the growth of human Staphylococcus are combined to make a simple emulsion for human trial.

[0021] The above technical solution is the first to study the physiological characteristics of the skin and the characteristics of the skin flora of people with oily skin. On this basis, the raw materials are screened and formulated based on the proliferation patterns of bacteria with higher abundance in oily skin. The safety of the formula and the skin improvement after use by the population are further verified. A composition suitable for the skin characteristics of people with oily skin is obtained and applied to products, which has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are merely embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without any creative work.

[0023] Figure 1 : Changes in skin physiological parameters among people with different sebum secretion levels;

[0024] Figure 2 : Changes in bacterial diversity among people with different sebum secretion levels;

[0025] Figure 3 : Composition of skin flora in people with different sebum secretion levels;

[0026] Figure 4 : Correlation analysis between skin physiological parameters and skin flora in oily people;

[0027] Figure 5 : Effects of different concentrations of suspension thickener raw materials on the growth of typical skin bacteria;

[0028] Figure 6 :The effects of oil and fat raw materials on the growth of typical skin bacteria;

[0029] Figure 7 : Effects of different concentrations of sodium hyaluronate on the growth of typical skin bacteria;

[0030] Figure 8 :Effects of carbohydrate raw materials on the growth of typical skin bacteria;

[0031] Figure 9 : Changes in skin flora characteristics of volunteers before and after using the product. DETAILED DESCRIPTION

[0032] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention belongs.

[0033] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0034] As mentioned earlier, although people with oily skin have always received widespread attention in the skincare industry and research has been conducted on species related to oily skin, no one has systematically studied the microbial composition and skin physiological parameters of people with oily skin, and the correlation between them. Even less has anyone specifically addressed the problems of oily skin from the perspective of maintaining microecological balance through skincare products.

[0035] In view of this, the present invention adopts a method that combines modern sequencing technology and traditional microbial culture to find the differences in the bacterial flora of people with oily skin, and uses three strains of bacteria with higher abundance on the skin as indicator bacteria to screen commonly used raw materials for cosmetics.

[0036] The research subjects selected for this invention are young female volunteers aged 18 to 35 years old. The research content complies with the approval of the Ethics Review Committee of Shanghai Fengxian District Central Hospital (Ethics Review No.: 2021-KY-15), the Declaration of Helsinki and the Good Clinical Practice of the People's Republic of China (GCP) and other relevant Chinese laws and regulations. The classification standard for oily skin people refers to the definition of oily people in the cosmetics oil control efficacy test method issued by the Zhejiang Health Products and Cosmetics Industry Association. It stipulates that the sebum content is greater than 120mg / cm within eight hours. 2 The population with oily skin is the oily skin population, so this study uses sebum secretion of 120mg / cm 2To establish the classification boundary, young female volunteers aged 18 to 35 were divided into two groups to study the physiological parameter levels and skin flora distribution of the two groups as well as the correlation between the two.

[0037] The study found that as the amount of sebum secretion increased, the skin water content of the two groups of people decreased, the transepidermal water loss increased, the melanin, hemoglobin and the a value reflecting the yellowness of the skin increased, and the skin became greasy, dull and dull overall; the study of the collected skin microorganisms found that as the amount of sebum secretion increased, the diversity and richness of microorganisms decreased significantly, the Propionibacterium and Staphylococcus genera increased, and the Pseudomonas, Neisseria, Rhodococcus, Streptococcus, Corynebacterium, Stenotrophomonas and Burkholderia decreased; by analyzing the skin physiological parameters and skin flora of people with oily skin Mann correlation analysis found that Propionibacterium was significantly negatively correlated with skin firmness, and significantly positively correlated with sebum content, hemoglobin and a value; Staphylococcus was significantly negatively correlated with gloss; Pseudomonas was significantly positively correlated with skin firmness and elasticity, and significantly negatively correlated with TEWL, sebum content, melanin and hemoglobin; Neisseria was significantly positively correlated with b value; Rhodococcus was significantly negatively correlated with sebum content; Streptococcus was significantly negatively correlated with TEWL value, sebum content and hemoglobin; Stenotrophomonas was significantly positively correlated with moisture, and significantly negatively correlated with hemoglobin and firmness. The above analysis shows that with the increase of sebum content, the skin lipophilic microorganisms Propionibacterium genus increased significantly, and Staphylococcus genus also showed an increasing trend. The massive proliferation of the two bacteria with higher abundance on the skin occupied the limited space and resources on the skin, resulting in a decrease in the richness of other species and a decrease in overall species diversity. Combined with the correlation analysis between the microbiome and skin physiological parameters, it can be found that the change in microbiome diversity fundamentally affects the change in skin condition, resulting in a decrease in skin water content, firmness, elasticity and glossiness, an increase in TEWL value, hemoglobin, melanin and a value, and the skin becomes dull, greasy and prone to allergies.

[0038] By analyzing changes in bacterial flora characteristics, the present invention screened three bacteria with high abundance from oily skin (Staphylococcus epidermidis CCSM0289, Propionibacterium acnes CCSM0333, and Staphylococcus hominis CCSM0283) as indicator bacteria to screen different types of commonly used cosmetic raw materials. Sugar and oil raw materials that can inhibit the growth of Staphylococcus epidermidis and Propionibacterium acnes while promoting the growth of Staphylococcus hominis were combined to make a simple emulsion for human trial.

[0039] Specifically, in a typical embodiment of the present invention, a composition for improving oily skin is provided. The present invention uses Staphylococcus epidermidis, Propionibacterium acnes, and Staphylococcus hominis as indicator bacteria, screens oil and sugar raw materials commonly used in cosmetics, combines the screened raw materials into a simple emulsion, recruits volunteers with excessive oil production for trial use, and analyzes the improvement in oily skin from the perspective of skin physiological parameters and skin flora characteristics. Ultimately, a group of cosmetic raw material formulas that can significantly improve excessive oil production and are friendly to skin flora are obtained. Specifically, the composition for improving oily skin comprises the following components by mass fraction:

[0040] 0.1%-2% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 1%-3% evening primrose oil, 1%-3% isononyl isononanoate, 1%-3% polydimethylsiloxane, 0.05%-0.2% sodium hyaluronate, 0.5%-1% D-mannitol, 1%-3% brown algae oligosaccharides, 0.01%-0.03% isomaltooligosaccharide, and the balance is water.

[0041] In another specific embodiment of the present invention, the molecular weight of the sodium hyaluronate is 1-2 million Da.

[0042] Furthermore, the composition for improving oily skin comprises the following components in mass fractions:

[0043] 0.5% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 2% evening primrose oil, 2% isononyl isononanoate, 1%-3% polydimethylsiloxane, 0.1% sodium hyaluronate, 1% D-mannitol, 2% algal oligosaccharides, 0.018% isomaltooligosaccharide, the balance is water.

[0044] The molecular weight of the sodium hyaluronate is 1.3 million Da.

[0045] In another embodiment of the present invention, the use of the above-mentioned composition for improving oily skin in the preparation of cosmetics is provided. Experiments have shown that the above-mentioned composition can significantly improve excessive oiliness and sebum secretion on the skin, is friendly to skin flora, and reduces potential inflammatory reactions on the skin.

[0046] Therefore, the cosmetic has at least one or more of the following effects:

[0047] (a) Effectively improve the composition of skin flora, increase skin species diversity and richness, and balance the skin microecology;

[0048] (b) Improve sebum secretion, increase skin moisture content, control transepidermal water loss rate and reduce a value;

[0049] (c) Reduce hemoglobin content, reduce the possibility of skin inflammation, and thus reduce the risk of skin further deteriorating into oily and acne-prone skin.

[0050] In another embodiment of the present invention, a cosmetic is provided, which comprises at least the above composition for improving oily skin.

[0051] The cosmetics may also contain any other raw material ingredients permitted in the cosmetics field, such as antioxidants, emulsifiers, thickeners, dispersants, pH regulators, preservatives, etc., which are not specifically limited here.

[0052] At the same time, the present invention can also be used to prepare different cosmetic dosage forms, such as water-based solutions, emulsions, gels, (creams), etc., by reasonably adding the above-mentioned raw materials. At the same time, other cosmetic categories can be further derived and prepared based on the above-mentioned basic cosmetic categories, which are also within the scope of protection of this application.

[0053] In another embodiment of the present invention, a method for preparing the above-mentioned cosmetic is provided, comprising: mixing the raw material components of the composition for improving oily skin; or, mixing the composition for improving oily skin with other raw material components.

[0054] In another embodiment of the present invention, there is provided use of the above composition and / or cosmetic for improving oily skin in any one or more of the following:

[0055] (a) Effectively improve the composition of skin flora, increase skin species diversity and richness, and balance the skin microecology;

[0056] (b) Improve sebum secretion, increase skin moisture content, control transepidermal water loss rate and reduce a value;

[0057] (c) Reduce hemoglobin content, reduce the possibility of skin inflammation, and thus reduce the risk of skin further deteriorating into oily and acne-prone skin.

[0058] The present invention is further described below with reference to the examples. The present invention is further described below by way of examples, but the present invention is not limited to the scope of the embodiments described. Based on the embodiments in the present invention, any changes to the present invention by those skilled in the art without making any creative ideas fall within the scope of protection of the present invention. At the same time, in the examples of the present invention, unless otherwise specified, all the raw materials for preparation are commercially available products well known to those skilled in the art. The three indicator bacteria selected in the examples, Staphylococcus epidermidis CCSM0289, Propionibacterium acnes CCSM0333 and Staphylococcus hominis CCSM0283, were all obtained by screening and separation from human skin by Shandong Freda Biological Co., Ltd.

[0059] The culture medium selected in the embodiment

[0060] TSA medium (g / L): 15.0 g of trypticase, 5.0 g of soy papain hydrolysate, 5.0 g of sodium chloride, and 15.0 g of agar. Sterilize by high pressure at 121°C for 15 min and set aside.

[0061] TSB medium (g / L): 17 g of tryptone, 3 g of soy peptone, 5 g of sodium chloride, 2.5 g of dipotassium hydrogen phosphate, and 2.5 g of glucose. Sterilize by autoclaving at 121°C for 15 min and set aside.

[0062] Basic culture medium (g / L): Tryptone 17, Soybean Peptone 3, Sodium Chloride 5,

[0063] K2HPO4 2.5, autoclave at 121℃ for 15 min and set aside.

[0064] Example 1 Analysis of skin physiological parameters and skin flora characteristics of people with different sebum secretion levels

[0065] (1) Volunteer recruitment and grouping

[0066] 180 female volunteers aged 18 to 35 were recruited. The exclusion criteria were as follows: 1. Those with a history of skin diseases such as psoriasis, eczema, atopic dermatitis, and allergies, as well as other systemic diseases; 2. Those who had taken oral or topical antibiotics, corticosteroids and other anti-inflammatory drugs in the past three months; 3. Pregnant or breastfeeding women, or those who have plans to get pregnant in the near future; 4. Those who are currently or have participated in other clinical trials in the past month; 5. Those who cannot complete the required content on time; 6. Other people who are deemed unsuitable for participation in the experiment by clinical assessment. All volunteers were informed of the purpose of the study, experimental procedures and potential risks, and signed informed consent forms. The sebum secretion of the recruited volunteers was measured, with a sebum secretion of 120 mg / cm 2 Categorize people based on boundaries.

[0067] (2) Physiological parameter index measurement

[0068] Before the test began, the subjects were asked to sit quietly in a constant temperature and humidity environment (temperature: (20±1)°C, relative humidity: 50%±5%) for 30 minutes. Skin physiological parameters (water content, transepidermal water loss rate, sebum content, melanin content, hemoglobin content, and skin Lab value) on the subjects' right cheek were then measured and recorded using a multifunctional skin tester from German company CK.

[0069] (3) Microbial sample collection and DNA extraction

[0070] Subjects were instructed to refrain from using any skincare products after washing their face the night before testing. Prior to testing, they were required to sit quietly for 30 minutes in a constant temperature and humidity environment (temperature: (20 ± 1)°C, relative humidity: 50% ± 5%). During testing, a sterile cotton swab was moistened with a moistening solution (0.9% NaCl + 0.1% Tween-20) and repeatedly scraped a 3 cm × 3 cm area on the subject's left cheek (at least 30 times), rotating the swab carefully during wiping. The swab was then placed into a collection tube. Microbial DNA was extracted from the skin swab using the FastDNA Spin Kit; detailed procedures are described in the kit's instructions. PCR amplification of the V3-V4 variable region of the bacterial 16S rRNA gene was performed using the upstream primer 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and the downstream primer 806R (5′-GGACTACHVGGGTWTCTAAT-3′). PCR products were purified using the AxyPrep DNA Gel Extraction Kit, libraries were constructed using the NEXTFLEX Rapid DNA-Seq Kit, and sequencing was performed using the MiSeq Reagent Kit. For specific steps, refer to the kit instructions.

[0071] (4) Data processing

[0072] Skin physiological parameters were analyzed using SPSS 25.0 software, and differences were considered statistically significant when P < 0.05. The composition of the skin microbial community was analyzed using the bio-cloud platform provided by Shanghai Meiji Biotechnology Co., Ltd.

[0073] The results are as follows Figure 1 、 2 As shown in Figures 3, 4, and 5, people with oily skin are characterized by low skin hydration, high transepidermal water loss, high melanin content, high hemoglobin content, and dull skin. In terms of microbial composition, the diversity of the oily skin microbiome is reduced, while the abundance of the more abundant Staphylococcus and Propionibacterium genera increases, leading to a decrease in the richness and diversity of other species and an imbalance in the skin microbiome. Correlation analysis between the microbiome and physiological parameters of people with oily skin revealed that Propionibacterium was significantly positively correlated with sebum content, hemoglobin, and a-value, and significantly negatively correlated with firmness; Staphylococcus was significantly negatively correlated with gloss. This suggests that the skin condition of people with high oil production is significantly correlated with changes in these two more abundant bacterial species.

[0074] Example 2 Screening of different concentrations of suspension thickeners in the composition

[0075] (1) Strain activation

[0076] The S. epidermidis CCSM0289 and S. hominis CCSM0283 strains were streaked onto TSA plates and cultured aerobically in a 37°C constant temperature incubator for 16 to 20 hours; the C. acnes CCSM0333 strain was streaked onto TSA plates and cultured anaerobically in a 37°C constant temperature incubator for 48 to 72 hours, activated twice, and set aside.

[0077] (2) Seed liquid culture

[0078] Single colonies of the activated two-generation S. epidermidis CCSM0289 and S. hominis CCSM0283 strains were picked and inoculated into TSB liquid culture medium, and cultured in a 37°C shaker (170 r / min) for 16 to 20 hours; two-generation activated C. acnes CCSM0333 strains were picked and inoculated into TSB liquid culture medium, and cultured in a 37°C constant temperature incubator for anaerobic culture for 48 to 72 hours, and the OD value of the fermentation broth was measured. 600 , adjust OD 600 The OD value of the culture medium is 0.5 to 0.7, which is used as seed solution. Different cosmetic raw materials are added to the basic culture medium (tryptone 17g / L, soy peptone 3g / L, sodium chloride 5g / L, K2HPO4 2.5g / L) according to the table below to culture the bacteria. The OD value after a certain period of time is 600 The colony proliferation rate was calculated by using the following formula:

[0079] {[(DC)-(BA)] / (BA)}*100%--------------------------------Formula 1

[0080] Table 1 Different culture medium addition conditions

[0081]

[0082] Different concentrations of sodium acrylate / sodium acryloyldimethyl taurate copolymer suspension thickener raw materials were screened, with screening concentrations of 0.125%, 0.25%, 0.5%, 1%, 2%, and 4%. Different concentrations of sodium acrylate / sodium acryloyldimethyl taurate copolymer were added to a basal culture medium. Seed liquids of three bacterial strains were inoculated at a 2% (v / v) inoculum into liquid culture medium containing different concentrations of sodium acrylate / sodium acryloyldimethyl taurate copolymer. The culture medium containing Staphylococcus epidermidis and Staphylococcus hominis was aerobically cultured for 16 hours and then removed, and the culture medium containing Propionibacterium acnes was cultured for 56 hours and then removed. The OD of the bacterial suspension was measured. 600 The proliferation of three bacteria in different concentrations of suspension thickeners was calculated.

[0083] Calculation results show that the growth of Staphylococcus epidermidis increases with increasing concentrations of sodium acrylate / sodium acryloyldimethyl taurate copolymer, showing an inhibitory trend at concentrations between 0.125% and 1%, and a growth trend at concentrations above 1%. Propionibacterium acnes is inhibited at concentrations between 0.125% and 4%, and the inhibitory trend becomes more pronounced with increasing concentrations. Staphylococcus hominis also increases with increasing concentrations of sodium acrylate / sodium acryloyldimethyl taurate copolymer, and shows a continuous positive growth trend. Comprehensively analyzing the growth of the three bacteria, sodium acrylate / sodium acryloyldimethyl taurate copolymer at concentrations of 0.5% and 1% exhibits inhibition against Staphylococcus epidermidis and P. acnes, but inhibits the growth of Staphylococcus hominis.

[0084] Example 3 Screening of oil and fat raw materials in the composition

[0085] Different types of oils and fats: peony seed oil, evening primrose oil, squalane, isononyl isononanoate, polydimethylsiloxane, tocopheryl acetate, organic flaxseed oil, and sweet almond oil were added to the basal culture medium at different concentrations for sterilization. The seed solution prepared above was inoculated into the culture medium containing different oils at a 2% (v / v) inoculum. The culture medium containing Staphylococcus epidermidis and Staphylococcus hominis was aerobically cultured for 16 hours and then removed. The culture medium containing Propionibacterium acnes was cultured for 56 hours and then removed. The OD value of the bacterial suspension was measured. 600 The proliferation of the two bacteria in different oil raw materials was calculated.

[0086] The calculation results show that peony seed oil and organic linseed oil exhibit different degrees of inhibitory effects on Staphylococcus epidermidis, while evening primrose oil and sweet almond oil exhibit different degrees of growth effects; for Propionibacterium acnes, evening primrose oil exhibits extremely strong inhibitory effects, isononyl isononanoate and polydimethylsiloxane also exhibit a certain degree of inhibitory effects, while peony seed oil, tocopheryl acetate, organic linseed oil and sweet almond oil exhibit a certain degree of growth effects; Staphylococcus hominis exhibits inhibitory effects on peony seed oil and organic linseed oil, and exhibits a certain degree of growth effects on evening primrose oil, isononyl isononanoate, polydimethylsiloxane and sweet almond oil.

[0087] Example 4 Screening of carbohydrate raw materials in the composition

[0088] Different types of sugar raw materials: xylitol, D-sorbitol, D-mannitol, galacto-oligosaccharides, isomaltooligosaccharides, inulin, brown algal oligosaccharides, and fucoidan, as well as different concentrations of 1.3 million molecular weight sodium hyaluronate were added to the basal culture medium for sterilization. The seed solution prepared above was inoculated into culture media containing different oils at a 2% (v / v) inoculation rate. The culture media containing Staphylococcus epidermidis and Staphylococcus hominis were aerobically cultured for 16 hours and then removed. The culture media containing Propionibacterium acnes were cultured for 56 hours and then removed. The OD value of the bacterial suspension was measured. 600The value calculation of the proliferation of the two bacteria in different sugar raw materials.

[0089] The calculation results show that for Staphylococcus epidermidis, xylitol, D-mannose, fucoidan and fucoidin show a certain degree of inhibition, galactose and inulin show a strong growth; for Propionibacterium acnes, xylitol, D-sorbitol, D-mannitol, isomaltulose and inulin show a certain degree of inhibition, and fucoidan shows a strong inhibition; for Staphylococcus hominis, mannitol, galactose, isomaltulose and fucoidan show different degrees of growth, and fucoidin shows inhibition. The utilization of 1.3 million molecular weight sodium hyaluronate of different concentrations by the three strains shows completely opposite results. With the increase of the concentration of sodium hyaluronate, Staphylococcus epidermidis shows growth at first and then inhibition, Propionibacterium acnes shows inhibition at first and then growth, and Staphylococcus hominis shows growth at first and then inhibition at 0.2% concentration. 1.3 million molecular weight sodium hyaluronate shows inhibition to Staphylococcus epidermidis and Propionibacterium acnes at a concentration of 0.1%, and still shows growth to Staphylococcus hominis.

[0090] Example 5 Formulation of a composition capable of effectively improving sebum secretion

[0091] According to the above screening results of different concentrations of suspending thickening agent, oil and sugar alcohol raw materials using Staphylococcus epidermidis 0289, Propionibacterium acnes 0333 and Staphylococcus hominis CCSM0283, three strains with high abundance, the composition is combined. The composition is as follows:

[0092] Table 2 Composition of different raw materials

[0093]

[0094] The above composition of Example 5 and Comparative Examples 1, 2 and 3 is used in emulsion type cosmetics. Four emulsions are prepared by the same preparation process except that different raw materials are used. The preparation process of the emulsion is as follows: dissolve the water-soluble raw materials (1.3 million molecular weight sodium hyaluronate, xylitol, D-mannitol, isomaltulose, fucoidan, sodium acrylate / sodium acryloyldimethyltaurate copolymer) in water and mix well, and keep at 85°C; dissolve and mix the oil-soluble raw materials (evening primrose oil, isononyl isononanoate, dimethicone, squalane), and keep at 85°C, then add the oil phase to the water phase, and homogenize at 4500 rpm for 4 minutes to obtain four emulsions. The emulsions added with the composition of Example 5 and Comparative Examples 1, 2 and 3 are named emulsion 1 (R1), emulsion 2 (R2), emulsion 3 (R3) and emulsion 4 (R4) respectively, and R0 represents the oily skin population before using the product.

[0095] Example 6: Recruiting volunteers to evaluate four lotions

[0096] Lotion testing was conducted on the oily skin population recruited in Example 1. The volunteers with oily skin were randomly divided into four groups and used Lotion 1, Lotion 2, Lotion 3, and Lotion 4, respectively. The volunteers replaced their usual lotion with this lotion twice daily, morning and evening. After 14 and 28 days of continuous use, the changes in facial sebum content were measured using a Sebumeter SM 815 sebum meter, changes in skin moisture content were measured using a CK skin moisture meter, and changes in facial transepidermal water loss rate were monitored using a Tewamater™ 300 DNA transepidermal water loss meter. Changes in facial Lab values ​​were measured using a Colorimeter CL 400 skin Lab value meter, and changes in facial hemoglobin content were monitored using a CK red-melanin probe. Microbial samples were collected from the volunteers' faces using sterile cotton swabs using the method described in Example 1. Changes in facial microbial flora after using the four lotions were compared.

[0097] Figure 9 Table 3 shows the changes in skin flora before and after using the four lotions. Table 3 shows the changes in sebum secretion, skin water content, transepidermal water loss rate, a value and hemoglobin content of the four groups of volunteers before and after using the four lotions.

[0098] The results showed that the four lotions not only improved sebum secretion, increased skin hydration, controlled transepidermal water loss, and lowered the a-value to a certain extent, but also reduced hemoglobin content to a certain extent, reducing the possibility of skin inflammation and the risk of further deterioration into oily and acne-prone skin. Lotion 1 was the most effective. The changes in the bacterial flora after using the four lotions showed that Lotion 1 effectively improved the bacterial composition, increased species diversity and richness, reduced the abundance of Propionibacterium and Staphylococcus, and balanced the skin microbiome. This shows that Formula 1 can effectively improve sebum secretion and reduce potential skin inflammation.

[0099] Table 3 Changes in skin physiological parameters after using four lotions for different periods of time

[0100]

[0101] It should be noted that the above examples are intended only to illustrate the technical solutions of the present invention and are not intended to limit the same. Although the present invention has been described in detail with reference to the examples given, those skilled in the art may modify or substitute equivalents for the technical solutions of the present invention as needed without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A composition for improving oily skin, characterized in that: The composition for improving oily skin comprises the following components by mass fraction: 0.5%-1% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 1%-3% evening primrose oil, 1%-3% isononyl isononanoate, 1%-3% dimethicone, 0.1%-0.2% sodium hyaluronate, 0.5%-1% D-mannitol, 1%-3% fucoidan, 0.01%-0.03% isomaltooligosaccharide, the balance is water; The molecular weight of the sodium hyaluronate is 1.3 million Da.

2. The composition according to claim 1, wherein The composition for improving oily skin comprises the following components by mass fraction: 0.5% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 2% evening primrose oil, 2% isononyl isononanoate, 1%-3% polydimethylsiloxane, 0.1% sodium hyaluronate, 1% D-mannitol, 2% brown algae oligosaccharides, 0.018% isomaltooligosaccharide, the balance is water.

3. Use of the composition for improving oily skin according to any one of claims 1 to 2 in the preparation of cosmetics.

4. The use according to claim 3, characterized in that The cosmetics have at least one or more of the following functions: (a) Effectively improve the composition of skin flora, increase skin species diversity and richness, and balance the skin microecology; (b) Improve sebum secretion, increase skin moisture content, control transepidermal water loss rate and reduce a value; (c) Reduce hemoglobin content, reduce the possibility of skin inflammation, and thus reduce the risk of skin further deteriorating into oily and acne-prone skin.

5. A cosmetic, characterized in that: The cosmetic comprises at least the composition for improving oily skin according to any one of claims 1 to 2.

6. The cosmetic according to claim 5, characterized in that The cosmetics are skin care products.

7. The method for preparing the cosmetic according to any one of claims 5 to 6, characterized in that: The preparation method comprises: mixing the raw material components of the composition for improving oily skin; or mixing the composition for improving oily skin with other raw material components.

Images