Children's traditional Chinese medicine ointment for strengthening spleen and replenishing qi, promoting body fluid and stimulating appetite and preparation method thereof

Abstract

The present invention provides a Chinese medicine ointment for children that can invigorate the spleen and replenish qi, promote the production of body fluid and stimulate appetite, and a preparation method thereof, and relates to the technical field of traditional Chinese medicine. The Chinese medicine ointment for children that can invigorate the spleen and replenish qi, promote the production of body fluid and stimulate appetite of the present invention comprises raw materials such as Poria cocos, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried tangerine peel, Codonopsis pilosula, Chinese yam, probiotic microcapsules, and defines the preparation method of the probiotic microcapsules. The present invention adopts the principle of compatibility of monarch, minister, assistant and envoy, and adopts a method combining water extraction and ethanol extraction to optimize the extraction conditions, ensure the full release and stability of the effective ingredients, and the introduction of probiotic microcapsules can protect the probiotics from the influence of Chinese medicinal materials, avoid inactivation, and prevent the ointment from precipitating during placement. The Chinese medicine ointment finally obtained by the present invention has the comprehensive health care functions of invigorating the spleen and replenishing qi, promoting the production of body fluid and stimulate appetite, and is suitable for daily health care and conditioning of children.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine, and in particular to a traditional Chinese medicine ointment for children capable of strengthening the spleen and replenishing qi, promoting the production of body fluids and stimulating appetite, and a preparation method thereof. Background Art

[0002] Spleen and stomach qi deficiency refers to the weakening of spleen and stomach function, which leads to symptoms such as loss of appetite, reduced food intake, and indigestion. Generally speaking, this situation is more common in children, because children's spleen and stomach functions are not fully developed and are easily affected by external factors, such as improper diet, emotional distress, and climate changes. Spleen and stomach qi deficiency not only affects children's digestion and absorption function, but may also lead to malnutrition, physical weakness, and decreased immunity.

[0003] Therefore, in the field of traditional Chinese medicine, the treatment of spleen and stomach qi deficiency should focus on strengthening the spleen and replenishing qi, and treat or improve the above symptoms by replenishing qi and strengthening the spleen, aiding digestion, and strengthening the physical fitness. In addition, it can also be combined with appropriate dietary conditioning, such as eating more easily digestible foods, avoiding raw, cold, greasy, and spicy foods, maintaining a regular diet, and eating small meals frequently. At the same time, pay attention to emotional conditioning, avoid excessive fatigue and mood swings, and maintain a happy mood, which is conducive to the recovery of spleen and stomach function. Through comprehensive conditioning, the symptoms of spleen and stomach qi deficiency can be effectively improved and the healthy growth of children can be promoted.

[0004] The invention patent with the publication number CN101108244A discloses a Chinese medicine prescription for invigorating qi and strengthening the spleen, which comprises the following raw materials in parts by weight: 30 parts of Astragalus, 12 parts of Codonopsis, 10 parts of Dry Ginger, 15 parts of Atractylodes, 12 parts of Poria, 12 parts of Tangerine Peel, 12 parts of White Cardamom, and 9 parts of Licorice. The Chinese medicine prescription for invigorating qi and strengthening the spleen has the effects of invigorating the spleen and replenishing qi, warming the middle and relieving pain. In the prescription, Astragalus, Codonopsis, and Atractylodes can invigorate the spleen and replenish qi, strengthen the body and consolidate the foundation; Dry Ginger, White Cardamom, and Tangerine Peel can warm the middle and dispel cold, regulate qi and relieve pain; Poria can invigorate the spleen and eliminate dampness; Licorice can harmonize the middle and replenish qi, and reconcile all the medicines. The above medicines are combined to play the role of invigorating the spleen and replenishing qi, warming the middle and relieving pain. Indications: chronic gastritis, peptic ulcer, chronic enteritis, gastroptosis, malnutrition after gastrectomy, gastrointestinal dysfunction, malabsorption syndrome, etc., which are caused by spleen yang deficiency. It can be seen that currently in the field of traditional Chinese medicine, pure Chinese herbal preparations are still chosen to regulate or treat spleen and stomach qi deficiency in children.

[0005] Probiotics are a type of active microorganisms that are beneficial to the host and are widely found in the human intestines, mouth, skin and other parts. They maintain human health by inhibiting the growth of harmful bacteria, promoting the digestion and absorption of nutrients, producing important nutrients (such as B vitamins), and regulating the function of the immune system. Common probiotics include lactic acid bacteria, bifidobacteria, yeast, etc. Bifidobacterium is a type of probiotic that mainly exists in the human intestine. They can ferment to produce lactic acid and acetic acid, inhibit the growth of harmful bacteria, maintain the acidic environment of the intestine, promote intestinal peristalsis, and help digestion and absorption of nutrients. Bifidobacteria can relieve the symptoms of spleen and stomach qi deficiency to a certain extent by regulating intestinal flora and improving intestinal function.

[0006] As a type of traditional Chinese medicine preparation, Chinese medicine ointment is easy to use, convenient to carry, and has various dosage forms. It is widely used in clinical Chinese medicine. The development of Chinese medicine ointment can be traced back to the records in ancient medical works. Medical scientists of all generations have accumulated rich experience in long-term clinical practice and formed many classic ointment prescriptions.

[0007] Therefore, how to combine Chinese medicine formulas, probiotics, and Chinese medicine ointments to obtain a product that has good therapeutic and improving effects on spleen and stomach qi deficiency, so as to achieve comprehensive treatment of spleen and stomach qi deficiency, is of great significance to the healthy growth of children. Summary of the invention

[0008] To achieve the above objectives, the present invention provides a Chinese medicine ointment for children that can strengthen the spleen and replenish qi, promote the production of body fluids and stimulate appetite, and a preparation method thereof. By adjusting the raw material ratio and preparation process of the formula, the effect and stability of the Chinese medicine ointment are improved.

[0009] The Chinese medicine ointment for children for strengthening the spleen and replenishing qi, promoting the production of body fluid and stimulating appetite of the present invention comprises the following raw materials in parts by weight:

[0010] 15-30 parts of Poria cocos, 15-30 parts of Atractylodes macrocephala, 15-30 parts of stir-fried hawthorn, 20-30 parts of roasted licorice, 30-40 parts of Euryale ferox, 30-40 parts of dried tangerine peel, 30-40 parts of Codonopsis pilosula, 30-50 parts of Chinese yam, and 5-10 parts of probiotic microcapsules.

[0011] Furthermore, the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following raw materials in parts by weight:

[0012] 20 parts of Poria cocos, 20 parts of Atractylodes macrocephala, 30 parts of stir-fried hawthorn, 20 parts of roasted liquorice, 35 parts of Euryale ferox, 35 parts of dried tangerine peel, 35 parts of Codonopsis pilosula, 40 parts of Chinese yam, and 8 parts of probiotic microcapsules.

[0013] The preparation method of the Chinese medicine ointment for children for strengthening spleen and replenishing qi, promoting body fluid and stimulating appetite of the present invention comprises the following steps:

[0014] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0015] S2, extracting the Poria powder, Atractylodes powder, stir-fried Hawthorn powder, roasted Licorice powder, Euryale powder, and Tangerine peel powder with water and filtering, repeating the extraction 2 to 3 times, and combining the filtrate to obtain a water extract;

[0016] S3, extracting the powder of Codonopsis pilosula and the powder of Atractylodes macrocephala with ethanol solution and filtering, repeating 2 to 3 times, and combining the filtrate to obtain an alcohol extract;

[0017] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0018] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0019] Furthermore, the weight-to-volume ratio of the raw material powder to water is 1 g:(5-15) mL. Preferably, the weight-to-volume ratio of the raw material powder to water is 1 g:10 mL.

[0020] Further, the water extraction temperature is 80°C to 100°C, and the water extraction time is 1 to 3 hours. Preferably, the water extraction temperature is 90°C to 95°C, and the water extraction time is 1 to 2 hours. More preferably, the water extraction temperature is 95°C, and the water extraction time is 1.5 hours.

[0021] Furthermore, the mass concentration of the ethanol solution is 70% to 95%. Preferably, the mass concentration of the ethanol solution is 80%.

[0022] Furthermore, the weight-to-volume ratio of the raw material powder to the ethanol solution is 1 g:(2-15) mL. Preferably, the weight-to-volume ratio of the raw material powder to the ethanol solution is 1 g:8 mL.

[0023] Furthermore, the ethanol extraction temperature is 60°C to 80°C. Preferably, the ethanol extraction temperature is 65°C.

[0024] Furthermore, the ethanol extraction is immersion extraction, reflux extraction or microwave extraction. Preferably, the ethanol extraction is microwave extraction.

[0025] Furthermore, when the ethanol extraction is immersion extraction or reflux extraction, the extraction time is 1 to 3 hours. Preferably, the extraction time is 2 hours.

[0026] Further, when the ethanol extraction is microwave extraction, the microwave power is 200-1000W, and the extraction time is 5-30min. Preferably, when the ethanol extraction is microwave extraction, the microwave power is 400-500W, and the extraction time is 10-20min. More preferably, when the ethanol extraction is microwave extraction, the microwave power is 450W, and the extraction time is 15min.

[0027] Furthermore, the preparation method of the probiotic microcapsule is as follows:

[0028] Resistant starch, whey protein and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, and then the probiotic solution, resistant starch solution and whey protein solution are mixed, dropped into the calcium chloride solution and stirred thoroughly to form microcapsules, rinsed with deionized water until no calcium chloride remains, and dried to obtain probiotic microcapsules.

[0029] Further, the concentration of the resistant starch solution is 2wt.% to 5wt.%, the concentration of the whey protein solution is 8wt.% to 15wt.%, and the concentration of the calcium chloride solution is 1wt.% to 3wt.%. Preferably, the concentration of the resistant starch solution is 3wt.%, the concentration of the whey protein solution is 10wt.%, and the concentration of the calcium chloride solution is 2wt.%.

[0030] Furthermore, the probiotics are bifidobacteria.

[0031] Furthermore, the probiotic microcapsule contains 10 8 ~10 11 CFU / g.

[0032] Furthermore, the Chinese medicine ointment can be made into a honey ointment by adding honey, crystal sugar, white sugar or maltose when the ointment is collected.

[0033] Compared with the prior art, the beneficial technical effects of the present invention are:

[0034] The invention adopts the principle of monarch-minister-assistant-assistant compatibility, combines multiple Chinese medicinal materials including Poria cocos, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, tangerine peel, Codonopsis pilosula and Atractylodes macrocephala, adopts a method combining water extraction and ethanol extraction, optimizes extraction conditions, and ensures full release and stability of effective ingredients.

[0035] The introduction of the probiotic microcapsules of the present invention can protect the probiotics from the influence of the Chinese medicinal materials and avoid inactivation.

[0036] The invention improves the stability of the traditional Chinese medicine ointment by adjusting the wall material of the probiotic microcapsule, thereby preventing the ointment from being precipitated during storage.

[0037] The Chinese medicinal ointment of the invention has the comprehensive health care functions of strengthening the spleen and replenishing qi, promoting the production of body fluids and stimulating appetite, and is suitable for daily health care and conditioning of children. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] The present invention will be further described below in conjunction with the accompanying drawings.

[0039] Figure 1 The present invention is a flow chart of the preparation process of the Chinese medicine ointment. DETAILED DESCRIPTION

[0040] The present invention provides a Chinese medicine ointment for children that can invigorate the spleen and replenish qi, promote the production of body fluids and stimulate appetite, and specifically comprises the following raw materials in parts by weight:

[0041] 15-30 parts of Poria cocos, 15-30 parts of Atractylodes macrocephala, 15-30 parts of stir-fried hawthorn, 20-30 parts of roasted licorice, 30-40 parts of Euryale ferox, 30-40 parts of dried tangerine peel, 30-40 parts of Codonopsis pilosula, 30-50 parts of Chinese yam, and 5-10 parts of probiotic microcapsules.

[0042] Poria cocos is the dried sclerotium of the Polyporaceae fungus Poria cocos (Schw.) Wolf. It tastes sweet and light, is neutral in nature, and enters the heart, lung, spleen, and kidney meridians. It has the effects of diuresis, invigorating the spleen, and calming the mind. It is used for edema, oliguria, phlegm and fluid, dizziness and palpitations, spleen deficiency, poor appetite, loose stools and diarrhea, restlessness, palpitations and insomnia.

[0043] Atractylodes is the dried rhizome of Atractylodes macrocephala Koidz., a plant of the Asteraceae family. It is warm in nature, bitter and sweet in taste, and enters the spleen and stomach meridians. It has the effects of tonifying Qi and strengthening the spleen, drying dampness and promoting diuresis, and stopping sweating. It is used to treat spleen deficiency, spleen deficiency and stagnation, spleen deficiency and fluid stagnation, phlegm and fluid, loose skin surface and excessive sweating, etc.

[0044] Hawthorn is the dried mature fruit of the Rosaceae plant Crataeguspin, natiida Bge.var.majorN.E.Br. or Crataeguspin.natifidaBge., which is sour, sweet, slightly warm, and enters the spleen, stomach, and liver meridians. The commonly used processed products of hawthorn are raw, fried, coke, and charcoal, etc. Different processing methods cause the chemical composition of the raw materials to change, resulting in different "nature and taste meridians" and "functions and indications" of different processed products. Therefore, the present invention limits the raw material of hawthorn to fried hawthorn, rather than raw hawthorn or hawthorn obtained by other processing methods. Fried hawthorn is obtained by heating raw hawthorn with medium heat, frying until the color deepens, taking out and cooling. There is no obvious change in the flavonoid components in hawthorn during this processing process, the organic acid content is reduced, and the sour taste is weakened, which can alleviate the irritation to the stomach and enhance the digestion and accumulation effect, while other types of hawthorn raw materials do not have this effect.

[0045] Licorice is the dried root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of the Leguminosae family. It is sweet, neutral in nature, and enters the heart, lung, spleen, and stomach meridians. Roasted licorice, also known as honey licorice, is prepared by diluting the boiled honey with boiling water, mixing it with licorice leaves, stirring it evenly, and then frying it over low heat until the color turns dark yellow, and drying it. Raw licorice is sweet and slightly cool, good at clearing heat and resolving phlegm, and is often used to treat lung heat cough, yellow phlegm, sore throat, carbuncle poison, food poisoning, and drug poisoning. Roasted licorice is sweet and slightly warm, and it can nourish the spleen and stomach, replenish qi and restore the pulse. It has a good therapeutic effect on symptoms such as spleen and stomach deficiency and cold, exhaustion, palpitations, and pulse knots. Therefore, the present invention limits the licorice to roasted licorice, and does not use raw licorice as medicine, which is similar to hawthorn.

[0046] Gorgon fruit is the dried mature seed kernel of Euryale ferox Salis b., a plant of the Nymphaeaceae family. It tastes sweet and astringent, is neutral in nature, and enters the spleen and kidney meridians. It has the effects of benefiting the kidney and consolidating essence, tonifying the spleen and stopping diarrhea, removing dampness and stopping leukorrhea, and is used for chronic diarrhea due to spleen deficiency.

[0047] Tangerine peel is the dried mature peel of the citrus (Citrus reticulata Blanco) and its cultivated varieties of the Rutaceae family. It tastes bitter and spicy, is warm in nature, and enters the lung and spleen meridians. It has the effects of regulating qi and strengthening the spleen, drying dampness and resolving phlegm. It is used for abdominal distension, loss of appetite, vomiting and diarrhea, and cough with excessive sputum.

[0048] Codonopsis pilosula is the dried root of Codonopsis pilosula, Codonopsis pilosula, or Codonopsis pilosula of the Campanulaceae family. It tastes sweet and is neutral in nature. It has the effects of tonifying the middle and replenishing qi, quenching thirst, strengthening the spleen and lungs, nourishing blood and producing body fluid. It is used for spleen and lung qi deficiency, poor appetite and fatigue, cough and asthma, insufficient qi and blood, sallow complexion, palpitations and shortness of breath, thirst due to loss of body fluid, and internal heat and thirst.

[0049] Yam is the dried rhizome of Dioscorea zingiberensis, a plant of the Dioscoreaceae family. It is sweet, warm, and neutral in nature. It enters the lung, spleen, and kidney meridians. It has the effects of nourishing the spleen and stomach, promoting fluid production and benefiting the lungs. It is used for spleen deficiency, poor appetite, long-term diarrhea, and cough and wheezing due to lung deficiency.

[0050] The monarch drugs in the prescription of the present invention are codonopsis and yam, which mainly play the role of invigorating qi and strengthening the spleen, the ministerial drugs include tuckahoe, atractylodes and euryale, which assist the monarch drugs in invigorating the spleen and replenishing qi, the adjuvant drugs are fried hawthorn and dried orange peel, which help digestion and eliminate accumulation, regulate the spleen and stomach, and the agent drug is roasted liquorice, which harmonizes various drugs, relieves urgency and stops pain, enhances the overall effect of the prescription, and reduces the toxicity and side effects of the drug. Through this compatibility mode, the spleen is moved to stimulate the appetite, the spleen is invigorated and qi is invigorated, the spleen and stomach are regulated, the absorption and utilization of the drug are enhanced, and good effects of invigorating the spleen and replenishing qi, promoting the production of body fluid and stimulating the appetite are achieved.

[0051] The present invention also adds a certain amount of probiotic microcapsules outside the Chinese medicine prescription. Probiotic microcapsules can provide probiotics, realize the combined conditioning of Chinese medicine and probiotics, and can avoid the use of multiple drugs at the same time, increasing the medication burden of children. However, the compounding of Chinese medicine ointment and probiotics causes the survival rate of probiotics to decrease, and the components in the Chinese medicine extract may interact with the probiotics, affecting the efficacy and other problems. For this reason, the present invention uses microcapsules to embed probiotics to avoid mutual influence between probiotics and Chinese medicine extracts, resulting in loss of viable bacteria and impaired efficacy, so as to ensure the good spleen-strengthening and qi-invigorating, body fluid-generating and appetizing effects of the product. In addition, the Chinese medicine ointment has complex ingredients, so there are generally poor stability, easy precipitation and other problems. The microcapsules of the present invention can improve the stability of the Chinese medicine ointment and play an anti-precipitation effect.

[0052] The present invention also provides a method for preparing the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, which specifically comprises the following steps:

[0053] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0054] S2, extracting the Poria powder, Atractylodes powder, stir-fried Hawthorn powder, roasted Licorice powder, Euryale powder, and Tangerine peel powder with water and filtering, repeating the extraction 2 to 3 times, and combining the filtrate to obtain a water extract;

[0055] S3, extracting the powder of Codonopsis pilosula and the powder of Atractylodes macrocephala with ethanol solution and filtering, repeating 2 to 3 times, and combining the filtrate to obtain an alcohol extract;

[0056] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0057] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0058] In one embodiment, the weight-to-volume ratio of the raw material powder to water is 1 g:(5-15) mL.

[0059] In one embodiment, the weight volume ratio of the raw material powder and water is 1 g:10 mL.

[0060] In one embodiment, the water extraction temperature is 80° C. to 100° C., and the water extraction time is 1 to 3 hours.

[0061] In one embodiment, the water extraction temperature is 90° C. to 95° C., and the water extraction time is 1 to 2 hours.

[0062] In one embodiment, the water extraction temperature is 95° C. and the water extraction time is 1.5 h.

[0063] In one embodiment, the mass concentration of the ethanol solution is 70% to 95%.

[0064] In one embodiment, the mass concentration of the ethanol solution is 80%.

[0065] In one embodiment, the weight-to-volume ratio of the raw material powder to the ethanol solution is 1 g: (2-15) mL.

[0066] In one embodiment, the weight volume ratio of the raw material powder to the ethanol solution is 1 g:8 mL.

[0067] In one embodiment, the ethanol extraction temperature is 60°C to 80°C.

[0068] In one embodiment, the ethanol extraction temperature is 65°C.

[0069] In one embodiment, the ethanol extraction is immersion extraction, reflux extraction or microwave extraction.

[0070] In one embodiment, the ethanol extraction is microwave extraction.

[0071] In one embodiment, when the ethanol extraction is immersion extraction or reflux extraction, the extraction time is 1 to 3 hours.

[0072] In one embodiment, the extraction time is 2 hours.

[0073] In one embodiment, when the ethanol extraction is microwave extraction, the microwave power is 200-1000 W, and the extraction time is 5-30 min.

[0074] In one embodiment, when the ethanol extraction is microwave extraction, the microwave power is 400-500W, and the extraction time is 10-20min.

[0075] In one embodiment, when the ethanol extraction is microwave extraction, the microwave power is 450 W and the extraction time is 15 min.

[0076] The present invention does not strictly limit the specific method of alcohol extraction, and conventional alcohol extraction methods in the art can be used in the present invention. However, from the perspective of extraction efficiency and extract quality, it is recommended to use a microwave extraction process for alcohol extraction.

[0077] Microwave-Assisted Extraction (MAE) is an emerging extraction technology used for the extraction of some raw materials of the composition of the present invention. It can significantly improve the extraction efficiency, shorten the extraction time, and protect heat-sensitive components. At the same time, microwave heating is a "body heating" process that can evenly heat the sample and avoid temperature gradient problems, which helps to improve the extraction efficiency and the quality of the extract. Microwaves can also selectively heat polar molecules, making it easier to separate the target components expected by the present invention from the matrix, thereby improving the extraction efficiency and product purity of the target extract. Therefore, in accordance with actual needs and when conditions permit, the use of microwave extraction to perform alcohol extraction of raw material powder can help save raw materials and improve extraction efficiency and extract quality.

[0078] In addition, the components of Chinese medicine are complex, and there are significant differences in the effective ingredients obtained by different extraction methods. The present invention adopts different extraction processes for different raw medicinal materials to maximize the extraction and retention of the effective ingredients in the Chinese medicinal materials, thereby ensuring the overall efficacy and stability of the pharmaceutical composition. At the same time, the screening of the composition of the effective ingredients and the regulation of the content of each component in the composition are achieved, overcoming the defects of complex components and unclear efficacy of Chinese medicine. Finally, appropriate extraction methods are carried out for different raw materials, which can greatly reduce the complexity of the raw materials of Chinese medicine ointments, further improve the stability of Chinese medicine ointments, and prevent precipitation.

[0079] In one embodiment, the preparation method of the probiotic microcapsule is as follows:

[0080] Resistant starch, whey protein and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, and then the probiotic solution, resistant starch solution and whey protein solution are mixed, dropped into the calcium chloride solution and stirred thoroughly to form microcapsules, rinsed with deionized water until no calcium chloride remains, and dried to obtain probiotic microcapsules.

[0081] In one embodiment, the concentration of the resistant starch solution is 2wt.% to 5wt.%, the concentration of the whey protein solution is 8wt.% to 15wt.%, and the concentration of the calcium chloride solution is 1wt.% to 3wt.%.

[0082] In one embodiment, the concentration of the resistant starch solution is 3 wt.%, the concentration of the whey protein solution is 10 wt.%, and the concentration of the calcium chloride solution is 2 wt.%.

[0083] In one embodiment, the probiotic is Bifidobacterium.

[0084] In one embodiment, the probiotic microcapsule contains 10 8 ~10 11 CFU / g.

[0085] The present invention uses composite wall materials to embed probiotics, and strictly limits the types and proportions of wall materials. First, the whey protein in the microcapsule wall material of the present invention helps to maintain uniform distribution and prevent the precipitation of insoluble components, and it has a certain antioxidant effect, which can prevent the active ingredients in the Chinese medicine ointment from oxidative degradation, thereby improving its stability. Through these mechanisms, whey protein can effectively improve the stability of the Chinese medicine ointment and prevent the generation of precipitation. In addition, the microcapsule wall material of the present invention also contains a certain amount of resistant starch, which can improve the stability of the probiotics in the microcapsule and prevent it from inactivating during storage and transportation. Resistant starch can also promote the growth and metabolism of bifidobacteria. Specifically, resistant starch can increase the density of bifidobacteria and the amount of biofilm generated, while promoting the production of short-chain fatty acids and extracellular polysaccharides. Finally, resistant starch can also increase the abundance of intestinal bacteria Bifidobacterium adolescentis, improve intestinal barrier function, and then strengthen the effect of Chinese medicine ointment.

[0086] In one embodiment, the Chinese medicine ointment can be made into a honey ointment by adding honey, crystal sugar, white sugar or maltose when the ointment is collected.

[0087] The technical solution provided by the present invention is further described below in conjunction with embodiments.

[0088] Example 1

[0089] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials:

[0090] 20g Poria cocos, 20g Atractylodes macrocephala, 30g stir-fried hawthorn, 20g roasted licorice root, 35g Euryale ferox, 35g dried tangerine peel, 35g Codonopsis pilosula, 40g Chinese yam, 8g probiotic microcapsule.

[0091] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein, and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 3wt.%, and a whey protein solution with a concentration of 10wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 2wt.% and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 10 9 CFU / g of probiotic microcapsules.

[0092] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0093] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0094] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g:10 mL, extract at 95°C for 1.5 h, then filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0095] S3, add 80% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, extract by microwave at 65°C, 450 W for 15 min, filter, repeat 2 to 3 times, and combine the filtrate to obtain an ethanol extract;

[0096] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0097] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0098] Example 2

[0099] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials:

[0100] 15g Poria cocos, 15g Atractylodes macrocephala, 15g stir-fried hawthorn, 20g roasted licorice root, 30g Euryale ferox, 30g dried tangerine peel, 30g Codonopsis pilosula, 30g Chinese yam, 5g probiotic microcapsule.

[0101] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 2wt.%, and a whey protein solution with a concentration of 8wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 1wt.% and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 10 9 CFU / g of probiotic microcapsules.

[0102] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0103] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0104] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g:10 mL, extract at 95°C for 1.5 h, then filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0105] S3, add 80% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, extract by microwave at 65°C, 450 W for 15 min, filter, repeat 2 to 3 times, and combine the filtrate to obtain an ethanol extract;

[0106] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0107] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0108] Example 3

[0109] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials:

[0110] 30g Poria cocos, 30g Atractylodes macrocephala, 30g stir-fried hawthorn, 30g roasted licorice, 40g Euryale ferox, 40g dried tangerine peel, 40g Codonopsis pilosula, 50g Chinese yam, 10g probiotic microcapsule.

[0111] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 5wt.%, and a whey protein solution with a concentration of 15wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 3wt.% and stirred thoroughly to form microcapsules, and the microcapsules are rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 10 9 CFU / g of probiotic microcapsules.

[0112] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0113] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0114] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g:10 mL, extract at 95°C for 1.5 h, then filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0115] S3, add 80% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, extract by microwave at 65°C, 450 W for 15 min, filter, repeat 2 to 3 times, and combine the filtrate to obtain an ethanol extract;

[0116] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0117] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0118] Comparative Example 1

[0119] Same as Example 1, except that the fried hawthorn is replaced with an equal amount of raw hawthorn.

[0120] Comparative Example 2

[0121] Same as Example 1, except that the roasted licorice root is replaced with an equal amount of raw licorice root.

[0122] Comparative Example 3

[0123] Same as Example 1, except that:

[0124] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0125] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0126] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, dried orange peel powder, Codonopsis powder, and Atractylodes powder at a weight volume ratio of 1g:10mL, extract at 95°C for 1.5h, filter, repeat the extraction 3 times, and combine the filtrates to obtain a water extract;

[0127] S3, concentrating the water extract to obtain a thick paste for later use;

[0128] S4. Mixing the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0129] Comparative Example 4

[0130] Same as Example 1, except that no probiotic microcapsules were added.

[0131] Comparative Example 5

[0132] Same as Example 1, except that an equal amount of bacterial liquid is directly added to the thick paste to obtain a traditional Chinese medicine ointment.

[0133] Comparative Example 6

[0134] Same as Example 1, except that whey protein is replaced by an equal amount of sodium alginate.

[0135] Comparative Example 7

[0136] Same as Example 1, except that whey protein is replaced by an equal amount of resistant starch.

[0137] Comparative Example 8

[0138] Same as Example 1, except that the resistant starch is replaced by an equal amount of whey protein.

[0139] Test Example 1: Stability Test

[0140] 50 mL of the product obtained in Examples 1-3 and Comparative Examples 1-8 was placed in a transparent glass container and sealed, and stored at 25°C in the dark (normal temperature) and 35°C in the dark (high temperature), respectively. After a specific period of time, the appearance properties were examined, the viscosity was measured and scored, and the scoring criteria are shown in Table 1. The scoring results are shown in Table 2, and the results are expressed as the sum of the two scores.

[0141] Table 1 Evaluation criteria for stability indicators

[0142]

[0143]

[0144] Table 2 Stability evaluation results

[0145]

[0146] Test Example 2 Effect Test

[0147] Under the premise of excluding allergies or intolerance to the Chinese medicine ointment of the present invention; accompanied by digestive system diseases; malnutrition, anemia; the presence of liver, kidney, hematopoietic system, cardiovascular and cerebrovascular diseases, etc., 55 children with spleen and stomach qi deficiency, loss of appetite, and decreased food intake were screened. After the parents of the children signed the consent form with knowledge, and proved that the children had not undergone relevant treatment or conditioning, they were randomly divided into 11 groups as experimental groups, 5 people in each group, and 5 healthy children (parents of the children signed the consent form with knowledge) as blank groups. The serum test results of Example 1 that did not start taking the Chinese medicine ointment were used as controls.

[0148] The children were aged 3 to 8 years old, with an average age of 5.6 years ± 0.4 years. Their body weight ranged from 10 to 33 kg, with an average weight of 23.3 kg ± 2.1 kg. Symptom duration ranged from 6 to 12 months, with an average of 9.3 months ± 1.5 months.

[0149] The experimental group orally took the Chinese medicine ointment of the embodiment and the comparative example, once in the morning and evening, 10 g each time, which could be taken with warm water, for 8 weeks, and the control group did not receive any treatment.

[0150] Fasting venous blood was drawn before and after oral administration of Chinese herbal ointment, and leptin (LP), serum neuropeptide (NPY), motilin (MTL), and gastrin (GAS) were determined by radioimmunoassay. The results are shown in Table 3.

[0151] Table 3 Blood test results

[0152] NPY(ng / L) LP (μg / L) MTL(ng / L) GAS(ng / L) Blank group 79.56±2.86 3.82±0.15 325.41±15.52 68.63±2.69 Control group 33.24±1.29 2.01±0.56 178.55±10.08 46.64±2.97 Example 1 79.16±1.52 3.80±0.21 323.78±17.91 68.29±3.23 Example 2 78.92±2.44 3.79±0.19 322.81±21.30 68.08±2.12 Example 3 79.28±3.31 3.81±0.13 324.27±26.26 68.39±2.80 Comparative Example 1 71.60±2.96 3.44±0.17 292.87±11.17 61.77±3.01 Comparative Example 2 73.20±2.15 3.51±0.21 299.38±27.73 63.14±1.55 Comparative Example 3 57.28±1.51 2.75±0.16 234.30±18.01 49.41±3.77 Comparative Example 4 62.06±1.09 2.98±0.14 253.82±13.59 53.53±2.33 Comparative Example 5 63.65±3.70 3.06±0.28 260.33±21.92 54.90±2.24 Comparative Example 6 79.14±4.33 3.80±0.30 323.69±29.35 68.27±5.39 Comparative Example 7 79.20±3.85 3.80±0.12 323.95±28.74 68.32±4.82 Comparative Example 8 70.01±3.61 3.36±0.29 286.36±22.68 60.39±3.65

[0153] Example 4

[0154] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials by weight:

[0155] 20g Poria cocos, 20g Atractylodes macrocephala, 30g stir-fried hawthorn, 20g roasted licorice root, 35g Euryale ferox, 35g dried tangerine peel, 35g Codonopsis pilosula, 40g Chinese yam, 8g probiotic microcapsule.

[0156] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein, and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 3wt.%, and a whey protein solution with a concentration of 10wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 2wt.% and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 10 9 CFU / g of probiotic microcapsules.

[0157] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0158] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0159] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g:10 mL, extract at 95°C for 1.5 h, then filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0160] S3, add 80% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, extract by microwave at 65°C, 450 W for 15 min, filter, repeat 2 to 3 times, and combine the filtrate to obtain an ethanol extract;

[0161] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0162] S5. Mix the thick paste with probiotic microcapsules and 10 g of honey to obtain a traditional Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0163] Example 5

[0164] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials by weight:

[0165] 20g Poria cocos, 20g Atractylodes macrocephala, 30g stir-fried hawthorn, 20g roasted licorice root, 35g Euryale ferox, 35g dried tangerine peel, 35g Codonopsis pilosula, 40g Chinese yam, 8g probiotic microcapsule.

[0166] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein, and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 3wt.%, and a whey protein solution with a concentration of 10wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 2wt.% and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 10 9 CFU / g of probiotic microcapsules.

[0167] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0168] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0169] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g: 5 mL, extract at 95 ° C for 1 h, then filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0170] S3, add 80% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, extract by microwave at 65°C, 450 W for 15 min, filter, repeat 2 to 3 times, and combine the filtrate to obtain an ethanol extract;

[0171] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0172] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0173] Example 6

[0174] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials by weight:

[0175] 20g Poria cocos, 20g Atractylodes macrocephala, 30g stir-fried hawthorn, 20g roasted licorice root, 35g Euryale ferox, 35g dried tangerine peel, 35g Codonopsis pilosula, 40g Chinese yam, 8g probiotic microcapsule.

[0176] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein, and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 3wt.%, and a whey protein solution with a concentration of 10wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 2wt.% and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 10 9 CFU / g of probiotic microcapsules.

[0177] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0178] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0179] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g:15 mL, extract at 90°C for 2 h, filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0180] S3, add 80% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, extract by microwave at 65°C, 450 W for 15 min, filter, repeat 2 to 3 times, and combine the filtrate to obtain an ethanol extract;

[0181] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0182] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0183] Example 7

[0184] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials by weight:

[0185] 20g Poria cocos, 20g Atractylodes macrocephala, 30g stir-fried hawthorn, 20g roasted licorice root, 35g Euryale ferox, 35g dried tangerine peel, 35g Codonopsis pilosula, 40g Chinese yam, 8g probiotic microcapsule.

[0186] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein, and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 3wt.%, and a whey protein solution with a concentration of 10wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 2wt.% and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 10 9 CFU / g of probiotic microcapsules.

[0187] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0188] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0189] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g:10 mL, extract at 95°C for 1.5 h, then filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0190] S3, adding 70% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, extracting by microwave at 65°C, 450 W for 15 min, and filtering, repeating 2 to 3 times, and combining the filtrate to obtain an ethanol extract;

[0191] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0192] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0193] Example 8

[0194] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials by weight:

[0195] 20g Poria cocos, 20g Atractylodes macrocephala, 30g stir-fried hawthorn, 20g roasted licorice root, 35g Euryale ferox, 35g dried tangerine peel, 35g Codonopsis pilosula, 40g Chinese yam, 8g probiotic microcapsule.

[0196] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein, and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 3wt.%, and a whey protein solution with a concentration of 10wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 2wt.% and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 10 11 CFU / g of probiotic microcapsules.

[0197] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0198] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0199] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g:10 mL, extract at 95°C for 1.5 h, then filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0200] S3, adding 90% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, extracting by microwave at 65°C, 450 W for 15 min, filtering, repeating 2 to 3 times, and combining the filtrate to obtain an ethanol extract;

[0201] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0202] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0203] Example 9

[0204] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials by weight:

[0205] 20g Poria cocos, 20g Atractylodes macrocephala, 30g stir-fried hawthorn, 20g roasted licorice root, 35g Euryale ferox, 35g dried tangerine peel, 35g Codonopsis pilosula, 40g Chinese yam, 8g probiotic microcapsule.

[0206] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein, and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 3wt.%, and a whey protein solution with a concentration of 10wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 2wt.% and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 10 10 CFU / g of probiotic microcapsules.

[0207] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0208] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0209] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g:10 mL, extract at 95°C for 1.5 h, then filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0210] S3, add 80% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, extract by microwave at 60°C, 450 W for 15 min, filter, repeat 2 to 3 times, and combine the filtrate to obtain an ethanol extract;

[0211] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0212] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0213] Example 10

[0214] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials by weight:

[0215] 20g Poria cocos, 20g Atractylodes macrocephala, 30g stir-fried hawthorn, 20g roasted licorice root, 35g Euryale ferox, 35g dried tangerine peel, 35g Codonopsis pilosula, 40g Chinese yam, 8g probiotic microcapsule.

[0216] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein, and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 3wt.%, and a whey protein solution with a concentration of 10wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 2wt.% and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 10 9 CFU / g of probiotic microcapsules.

[0217] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0218] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0219] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g:10 mL, extract at 95°C for 1.5 h, then filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0220] S3, add 80% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, extract by microwave at 80°C, 450 W for 15 min, filter, repeat 2 to 3 times, and combine the filtrate to obtain an ethanol extract;

[0221] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0222] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0223] Embodiment 11

[0224] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials by weight:

[0225] 20g Poria cocos, 20g Atractylodes macrocephala, 30g stir-fried hawthorn, 20g roasted licorice root, 35g Euryale ferox, 35g dried tangerine peel, 35g Codonopsis pilosula, 40g Chinese yam, 8g probiotic microcapsule.

[0226] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein, and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 3wt.%, and a whey protein solution with a concentration of 10wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 2wt.% and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 10 9 CFU / g of probiotic microcapsules.

[0227] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0228] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0229] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g:10 mL, extract at 95°C for 1.5 h, then filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0230] S3, add 80% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, reflux extract at 65° C. for 2 h, filter, repeat 2 to 3 times, and combine the filtrate to obtain an ethanol extract;

[0231] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0232] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0233] Example 12

[0234] A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, and is composed of the following raw materials by weight:

[0235] 20g Poria cocos, 20g Atractylodes macrocephala, 30g stir-fried hawthorn, 20g roasted licorice root, 35g Euryale ferox, 35g dried tangerine peel, 35g Codonopsis pilosula, 40g Chinese yam, 8g probiotic microcapsule.

[0236] The preparation method of the probiotic microcapsules is as follows: resistant starch, whey protein, and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, then a bifidobacterium bacterial solution, a resistant starch solution with a concentration of 3wt.%, and a whey protein solution with a concentration of 10wt.% are mixed, and the mixture is dropped into a calcium chloride solution with a concentration of 2wt.% and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain a microcapsule with a bacterial content of 109 CFU / g of probiotic microcapsules.

[0237] The preparation method of the Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite comprises the following steps:

[0238] S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use;

[0239] S2, add water to Poria powder, Atractylodes powder, stir-fried hawthorn powder, roasted licorice powder, Euryale powder, and dried orange peel powder at a weight volume ratio of 1 g:10 mL, extract at 95°C for 1.5 h, then filter, repeat the extraction 3 times, and combine the filtrate to obtain a water extract;

[0240] S3, adding 80% ethanol solution to Codonopsis pilosula powder and Atractylodes macrocephala powder in a weight volume ratio of 1 g: 8 mL of ethanol solution, soaking and extracting at 65°C for 2 h and filtering, repeating 2 to 3 times, and combining the filtrate to obtain an ethanol extract;

[0241] S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use;

[0242] S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

[0243] The principles and implementation methods of the present invention are described in this article using specific examples. The description of the above embodiments is only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite, characterized in that: It is composed of the following raw materials in parts by weight: 15-30 parts of Poria cocos, 15-30 parts of Atractylodes macrocephala, 15-30 parts of stir-fried hawthorn, 20-30 parts of roasted licorice root, 30-40 parts of Euryale ferox, 30-40 parts of dried tangerine peel, 30-40 parts of Codonopsis pilosula, 30-50 parts of Chinese yam, 5-10 parts of probiotic microcapsules; The preparation method of the probiotic microcapsule is as follows: Resistant starch, whey protein and calcium chloride are respectively dissolved in deionized water and stirred evenly to obtain corresponding solutions, and then the probiotic solution, resistant starch solution and whey protein solution are mixed, and the mixture is dropped into the calcium chloride solution and stirred thoroughly to form microcapsules, and the mixture is rinsed with deionized water until no calcium chloride remains, and dried to obtain probiotic microcapsules; The preparation method of the Chinese medicine ointment comprises the following steps: S1, drying and crushing Poria, Atractylodes macrocephala, stir-fried hawthorn, roasted licorice, Euryale ferox, dried orange peel, Codonopsis pilosula, and Atractylodes macrocephala in turn to obtain powder for later use; S2, extracting Poria powder, Atractylodes macrocephala powder, stir-fried hawthorn powder, roasted liquorice powder, Euryale ferox powder, and dried orange peel powder with water, filtering, repeating the extraction 2 to 3 times, and combining the filtrate to obtain a water extract; S3, extracting the powder of Codonopsis pilosula and the powder of Atractylodes macrocephala with ethanol solution and filtering, repeating 2 to 3 times, and combining the filtrate to obtain an ethanol extract; S4, respectively concentrating the water extract and the alcohol extract to obtain an alcohol extract concentrate and a water extract concentrate, mixing the concentrates to obtain a thick paste for standby use; S5. Mix the thick paste with probiotic microcapsules to obtain a children's traditional Chinese medicine ointment that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite.

2. The Chinese medicine ointment for children for strengthening the spleen and replenishing qi, promoting body fluid and stimulating appetite according to claim 1, characterized in that: The concentration of the resistant starch solution is 2wt.%~5wt.%, the concentration of the whey protein solution is 8wt.%~15wt.%, and the concentration of the calcium chloride solution is 1wt.%~3wt.%.

3. The Chinese medicine ointment for children that strengthens the spleen and replenishes qi, promotes the production of body fluids and stimulates appetite according to claim 1, wherein the concentration of the resistant starch solution is 3wt.%, the concentration of the whey protein solution is 10wt.%, and the concentration of the calcium chloride solution is 2wt.%.

4. The Chinese medicine ointment for children for strengthening the spleen and replenishing qi, promoting body fluid and stimulating appetite according to claim 1, characterized in that: The water extraction temperature is 80°C to 100°C, and the water extraction time is 1 to 3 hours.

5. The Chinese medicine ointment for children for strengthening the spleen and replenishing qi, promoting body fluid and stimulating appetite according to claim 1, characterized in that: The ethanol extraction temperature is 60°C to 80°C.

6. The Chinese medicine ointment for children for strengthening the spleen and replenishing qi, promoting body fluid and stimulating appetite according to claim 1, characterized in that: The ethanol extraction is immersion extraction, reflux extraction or microwave extraction.

7. The Chinese medicine ointment for children for strengthening the spleen and replenishing qi, promoting body fluid and stimulating appetite according to claim 1, characterized in that: The probiotics are bifidobacteria.

8. The Chinese medicine ointment for children for strengthening the spleen and replenishing qi, promoting body fluid and stimulating appetite according to claim 1, characterized in that: The probiotic microcapsule contains 10 8 ~10 11 CFU / g.

9. The Chinese medicine ointment for children for strengthening the spleen and replenishing qi, promoting body fluid and stimulating appetite according to claim 1, characterized in that: Step S4: adding honey, crystal sugar, white sugar or maltose to obtain honey paste.

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