Agricultural composition containing hemiphyllin and brown algae oligosaccharide

Abstract

The present invention discloses an agricultural composition containing hemiphyllin and brown algae oligosaccharide, belonging to the technical field of agricultural plant growth regulators. The agricultural composition provided by the present invention comprises hemiphyllin and brown algae oligosaccharide as active ingredients, and the mass ratio of the effective ingredients of hemiphyllin and brown algae oligosaccharide is 4 to 14:10 to 35. After the hemiphyllin and brown algae oligosaccharide are compounded and applied to rice, the quality and yield of the rice are improved. Within the ratio range provided by the present invention, the number of grains per ear, the fruit set rate, the polished rice rate and the thousand-grain weight of the rice are all well improved, and the yield of the rice is increased by 15.65 to 19.90%. Within the ratio range provided by the present invention, the agricultural composition has a synergistic effect on the increase of rice yield.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators and relates to an agricultural composition containing hemichlorophyllin and brown algae oligosaccharide. Background Art

[0002] Semi-phyllin, also known as N-acetylthioproline, was originally a pharmaceutical intermediate with protective effects against myocardial ischemia. Recent studies have found that semi-phyllin has growth-regulating functions similar to cytokinins, primarily affecting the cell division and growth processes of plant growth, promoting the synthesis and accumulation of proteins, nucleic acids, and alkaloids within the plant body. It also influences hormonal balance, improving plant adaptability to the environment and enhancing resistance to drought, cold, and disease. Semi-phyllin can regulate crop growth, promote seed germination, increase fruit set and yield, and ultimately increase crop yield.

[0003] Fucoidan is a low-molecular-weight fragment obtained by degradation of fucoidan (algin) through acid, oxidants, or lyase. It is a linear block polymer composed of mannuronic acid and guluronic acid. Fucoidan can induce the formation of callus tissue, significantly increase the content of hormones (such as indoleacetic acid) in cells, and promote seed germination, seedling growth, and rooting. Fucoidan can also induce the secretion of plant growth hormones such as abscisic acid in plants to 5 to 7 times the normal level, significantly improving the plant's stress resistance. Fucoidan is an endogenous plant oligosaccharide that can participate in plant growth regulation and induce disease resistance. It acts as a signaling molecule in plant stress resistance and can form a protective film to effectively protect protein molecules from denaturation and inactivation, thereby maintaining the life processes and biological characteristics of living organisms and demonstrating resistance to harsh external environments.

[0004] Different plant growth regulators have different mechanisms of action, and mixing them can enhance their interactions. However, due to the different mechanisms of action of ramifolic acid and fucoidan oligosaccharides, there are no reports of combining ramifolic acid and fucoidan oligosaccharides to regulate crop growth in crop production. Therefore, developing a compound agricultural composition containing ramifolic acid and fucoidan oligosaccharides as active ingredients that can promote crop growth and development, and increase crop yield and quality is of great significance. Summary of the Invention

[0005] To develop an agricultural composition containing hemiphyllin and fucoidan oligosaccharides as active ingredients to promote crop growth and development, and improve crop yield and quality, the present invention provides an agricultural composition containing hemiphyllin and fucoidan oligosaccharides. The weight ratio of the active ingredients of hemiphyllin to fucoidan oligosaccharides in the agricultural composition is 4-14:10-35. Within the ratio range provided by the present invention, the agricultural composition significantly improves both rice yield and quality, and the agricultural composition provided by the present invention also has a synergistic effect on increasing rice yield.

[0006] To achieve the technical objectives of the present invention, in one aspect, the present invention provides an agricultural composition, wherein the active ingredients of the agricultural composition are composed of schizolin and fucoidan oligosaccharides. The weight ratio of the active ingredients of schizolin to fucoidan oligosaccharides in the agricultural composition is 4-14:10-35; preferably, the weight ratio of the active ingredients of schizolin to fucoidan oligosaccharides in the agricultural composition is 8-12:15-25; further preferably, the weight ratio of the active ingredients of schizolin to fucoidan oligosaccharides in the agricultural composition is 8:25.

[0007] It is easy for those skilled in the art to know that in order to give full play to the efficacy of the active ingredients of plant growth regulators (pesticides), they can be prepared into formulations that are easy to apply according to the needs of use. The agricultural composition is processed into a formulation that is accepted in agriculture, and contains adjuvants that assist the hemiphyllin and brown algae oligosaccharides in regulating the growth of crops. The agricultural composition can be formulated into various formulations such as aqueous solutions, soluble liquids, water-dispersible granules, wettable powders, dispersible oil suspensions, suspensions, microemulsions, granules, and microcapsules with a certain sustained-release effect. When preparing the above-mentioned different formulations, for those skilled in the art, in addition to using the plant growth regulator active ingredients to be selected, it is also necessary to select a variety of adjuvants, and different formulation auxiliary ingredients (auxiliaries) can be selected and used as needed. The auxiliary ingredients can be one or more of a dispersion medium, a dispersant, an emulsifier, a wetting agent, a thickener, a defoaming agent, an antifreeze, a disintegrant, a binder, a filler, a carrier, etc.

[0008] On the other hand, the present invention seeks to protect the use of the above-mentioned agricultural composition in improving the quality of rice, and improving the quality of rice includes increasing the number of grains per panicle, the fruit setting rate, the whole-headed rice rate and the thousand-grain weight of rice.

[0009] Furthermore, after applying the agricultural composition to rice, the present invention found that after compounding the effective ingredients of hemiphyllin and brown algae oligosaccharide according to a mass ratio of 4 to 14:10 to 35, the number of grains per ear, the fruit set rate, the whole polished rice rate and the thousand-grain weight of the rice were all greatly improved. The number of grains per ear of rice increased by 5.20 to 9.91%, the fruit set rate of rice increased by 4.30 to 5.26%, and the whole polished rice rate of rice increased by 9.88 to 13.34%, indicating that the application of the agricultural composition of the present invention can improve the quality of rice, and the effect of improving the quality of rice is best when the effective ingredients of hemiphyllin and brown algae oligosaccharide are compounded in a mass ratio of 8:25.

[0010] Furthermore, the present invention claims protection for the use of the above agricultural composition in increasing crop yield, wherein the crop is rice.

[0011] Furthermore, after applying the agricultural composition to rice, the present invention found that it also had a synergistic effect within the ratio range provided in the present application. When the active ingredients of hemiphyllin and brown algae oligosaccharide were compounded in the ratio range of 4-14:10-35, they had a yield-increasing effect on rice. The agricultural composition had a yield-increasing rate of 15.65-19.90% on rice, and the difference between the actual yield increase rate and the theoretical yield increase rate within this ratio range was greater than 10%, showing a synergistic effect. When the active ingredients of hemiphyllin and brown algae oligosaccharide were in the ratio range of 8-12:15-25, the agricultural composition had a better yield-increasing effect on rice, with the yield increase rate reaching more than 18%, and the difference between the actual yield increase rate and the theoretical yield increase rate was greater than 11%, indicating a better synergistic effect. When the effective ingredients of hemiphyllin and brown algae oligosaccharide were in the ratio of 8:25, the synergistic effect was optimal, with the difference between the actual yield increase rate and the theoretical yield increase rate being 12.21%.

[0012] The agricultural composition comprising hemiphyllin and fucoidan oligosaccharides is intended for use in crops requiring growth promotion or quality improvement, more preferably, rice. Based on the teachings of the present invention, the agricultural composition comprising hemiphyllin and fucoidan oligosaccharides can also be used in field crops.

[0013] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0014] The agricultural composition containing ramifolic acid and brown algae oligosaccharide of the present invention has the effect of improving the quality of rice. After ramifolic acid and brown algae oligosaccharide are compounded according to the effective ingredient mass ratio of 4-14:10-35, the number of grains per ear, the fruit setting rate, the whole polished rice rate and the thousand-grain weight of rice are all greatly improved. The number of grains per ear of rice increased by 5.20-9.91%, the fruit setting rate of rice increased by 4.30-5.26%, and the whole polished rice rate of rice increased by 9.88-13.34%, indicating that the application of the agricultural composition of the present invention can improve the quality of rice, and the effect of improving the quality of rice is best when the effective ingredient compounding mass ratio of ramifolic acid and brown algae oligosaccharide is 8:25. When the compounding ratio of ramifolic acid and brown algae oligosaccharide changes, the effect of improving the quality of rice also changes accordingly, indicating that although ramifolic acid and brown algae oligosaccharide are both plant growth regulators, there are differences in the effects they play. The application of hemiphyllin or brown algae oligosaccharide alone also has a promoting effect on improving rice quality, but the promoting effect is worse than that of the compounded agricultural composition of the present invention; when the ratio range provided by the present invention is exceeded, the effect of the agricultural composition on improving rice quality becomes worse; after replacing brown algae oligosaccharide with marine oligosaccharide chitosan, the effect of the agricultural composition on improving rice quality is reduced.

[0015] The agricultural composition containing hemiphyllin and fucoidan oligosaccharide of the present invention has the effect of increasing rice yield and also has a synergistic effect within the ratio range provided in the present application. When the effective components of hemiphyllin and fucoidan oligosaccharide are compounded in the ratio range of 4-14:10-35, they have a rice yield-increasing effect. The agricultural composition has a rice yield-increasing rate of 15.65-19.90%, and the difference between the actual yield increase rate and the theoretical yield increase rate within this ratio range is greater than 10%, showing a synergistic effect. When the effective components of hemiphyllin and fucoidan oligosaccharide are in the ratio range of 8-12:15-25, the agricultural composition has a better rice yield-increasing effect, with the yield increase rate reaching more than 18%, and the difference between the actual yield increase rate and the theoretical yield increase rate is greater than 11%, indicating a better synergistic effect. When the effective components of hemiphyllin and fucoidan oligosaccharide are in the ratio of 8:25, the synergistic effect is optimal, and the difference between the actual yield increase rate and the theoretical yield increase rate is 12.21%. When the ratio range provided by the present invention is exceeded or the components of the agricultural composition of the present invention are changed, the yield increase rate of rice is additive, and the effect is worse than that of the agricultural composition of the present invention. DETAILED DESCRIPTION

[0016] The technical solutions of the present invention are described below with reference to the following examples. However, the present invention is not limited to the following examples. The experimental methods and detection methods described in each example are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.

[0017] Hemiphyllin was purchased from Shandong Lifan Chemical Co., Ltd. with a purity of 99%.

[0018] Brown algae oligosaccharide was purchased from Wuhan Rongcan Biotechnology Co., Ltd. with a purity of 99%.

[0019] Chitosan was purchased from Shandong Haiyihua Biotechnology Co., Ltd. with a purity of 90%.

[0020] Example 1

[0021] This example provides a field test on the effect of an agricultural composition containing fusin and fucoidan on rice.

[0022] Semi-phyllin and brown algae oligosaccharide were prepared into solutions respectively, and were sprayed on the leaves of rice at the early tillering stage and the filling stage respectively using a backpack electric sprayer, with a spraying amount of 15L / mu. After spraying the agricultural composition, the management was the same as that of the field.

[0023] The test site was the Zuhai rice planting base in Zhongxiang City, Hubei Province. The rice variety tested was Shenliangyou 534. The paddy fields were well managed for fertilizer and water. The plot test method was adopted, and the test plot area was 40m 2 The plots were randomly arranged in blocks, separated by ditches, and repeated three times. The seedlings were transplanted in mid-to-late April with a planting density of 26.7 cm × 20 cm, 4 to 6 seedlings per clump.

[0024] The experiment involved a total of 26 treatments, namely, Experiments 1 to 8: only application of schizolin; Experiments 9 to 16: only application of brown algae oligosaccharides; Experiment 17: only application of chitosan; Experiment 18: the mass ratio of the effective ingredients of schizolin and brown algae oligosaccharides was 2:40; Experiment 19: the mass ratio of the effective ingredients of schizolin and brown algae oligosaccharides was 4:35; Experiment 20: the mass ratio of the effective ingredients of schizolin and brown algae oligosaccharides was 6:30; Experiment 21: the mass ratio of the effective ingredients of schizolin and brown algae oligosaccharides was 8:25; Experiment 2 2: The mass ratio of the effective ingredients of hemiphyllin and brown algae oligosaccharide was 10:20; Experiment 23: The mass ratio of the effective ingredients of hemiphyllin and brown algae oligosaccharide was 12:15; Experiment 24: The mass ratio of the effective ingredients of hemiphyllin and brown algae oligosaccharide was 14:10; Experiment 25: The mass ratio of the effective ingredients of hemiphyllin and brown algae oligosaccharide was 16:5; Experiment 26: Brown algae oligosaccharide was replaced by chitosan, and the mass ratio of the effective ingredients of hemiphyllin and chitosan was 8:25; Experiment 27: An equal amount of water was applied, which served as a blank control group. A random five-point sampling method was used in each plot, and 20 clusters were surveyed at each point. The number of rice grains per ear, fruit setting rate, head rice rate, and 1,000-grain weight were recorded. The statistical results are shown in Table 1; the total yield of rice in different experimental groups was statistically analyzed, and the statistical results are shown in Table 2. The yield increase calculation formula in Table 2 is as follows:

[0025] Actual yield increase rate E (%) = [(yield of the drug treatment group - yield of the blank control group) / yield of the blank control group] × 100

[0026] Theoretical yield increase of the combined application of two agents E0 (%) = X + Y - XY / 100

[0027] In the formula, X represents the actual yield increase of hemiphyllin when the dosage is A; Y represents the actual yield increase of brown algal oligosaccharide when the dosage is B; the combined action type of the mixture is evaluated according to the Gowing method in NY / T2061.5-2016, with E-E0>10% indicating a synergistic effect, E-E0<-10% indicating an antagonistic effect, and an E-E0 value within ±10% indicating an additive effect.

[0028] Table 1: Effects of agricultural compositions containing haloperidol (A) and fucoidan (B) on rice

[0029]

[0030]

[0031] As shown in Table 1, after compounding the effective ingredient mass ratio of 4-14:10-35 of hemiphyllin and brown algae oligosaccharide, the number of rice grains per ear, the fruit set rate, the polished rice rate and the thousand-grain weight were all significantly improved. The number of rice grains per ear increased by 5.20-9.91%, the fruit set rate of rice increased by 4.30-5.26%, and the polished rice rate of rice increased by 9.88-13.34%, indicating that the application of the agricultural composition of the present invention can improve the quality of rice, and the effect of improving rice quality is best when the effective ingredient compounding mass ratio of hemiphyllin and brown algae oligosaccharide is 8:25. When the compounding ratio of hemiphyllin and brown algae oligosaccharide changes, the effect of improving rice quality also changes accordingly, indicating that although hemiphyllin and brown algae oligosaccharide are both plant growth regulators, there are differences in the effects they play. The application of hemiphyllin or brown algae oligosaccharide alone also has a promoting effect on improving rice quality, but the promoting effect is worse than that of the compounded agricultural composition of the present invention; when the ratio range provided by the present invention is exceeded, the effect of the agricultural composition on improving rice quality becomes worse; after replacing brown algae oligosaccharide with marine oligosaccharide chitosan, the effect of the agricultural composition on improving rice quality is reduced.

[0032] Table 2: Effects of agricultural compositions containing haloperidol (A) and fucoidan (B) on rice

[0033]

[0034]

[0035] As shown in Table 2, the effective components of hemiphyllin and brown algae oligosaccharide have an increasing effect on rice yield when compounded in a ratio range of 4-14:10-35. The agricultural composition has an increasing yield rate of 15.65-19.90% on rice, and the difference between the actual yield increase rate and the theoretical yield increase rate within this ratio range is greater than 10%, showing a synergistic effect. When the effective components of hemiphyllin and brown algae oligosaccharide are in a ratio range of 8-12:15-25, the agricultural composition has a better increasing effect on rice yield, with the yield increase rate reaching more than 18%, and the difference between the actual yield increase rate and the theoretical yield increase rate is greater than 11%, showing a better synergistic effect. When the effective component ratio of hemiphyllin and brown algae oligosaccharide is 8:25, the synergistic effect is the best, with the difference between the actual yield increase rate and the theoretical yield increase rate being 12.21%. When the ratio range provided by the present invention is exceeded or the components of the agricultural composition of the present invention are changed, the yield increase rate of rice shows an additive effect, and the effect is worse than that of the agricultural composition of the present invention.

[0036] The embodiments described above are some of the embodiments of the present invention, rather than all of them. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but rather merely represents selected embodiments of the present invention. All other embodiments obtained without creative effort and through deduction and substitution by a person of ordinary skill in the art based on the concept of the present invention are within the scope of protection of the present invention.

Claims

1. An agricultural composition, characterized in that The effective components of the agricultural composition are composed of hemiphyllin and brown algae oligosaccharide, and the effective component mass ratio of hemiphyllin to brown algae oligosaccharide is 4-14:10-35.

2. The agricultural composition according to claim 1, characterized in that The mass ratio of the effective ingredients of hemiphyllin and fucoidan in the agricultural composition is 8-12:15-25.

3. The agricultural composition according to claim 1, characterized in that The mass ratio of the effective ingredients of hemiphyllin and fucoidan in the agricultural composition is 8:

25.

4. Use of the agricultural composition according to any one of claims 1 to 3 for improving rice quality, characterized in that: Improving the rice quality includes improving the number of grains per panicle, the fruit setting rate, the head rice rate and the thousand-grain weight of the rice.

5. Use of the agricultural composition according to any one of claims 1 to 3 in increasing crop yield.

6. The use according to claim 5, characterized in that The crop is rice.

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